detection of a nuclear, ebna-type antigen in apparently ebna-negative herpesvirus papio (hvp)-transformed lymphoid lines by the acid-fixed nuclear binding agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in herpesvirus papio (hvp)-transformed baboon lymphoid lines by anticomplementary staining in situ, as for ebna. however, by means of our recently developed acid-fixed nuclear binding technique an ebna-like antigen could be readily demonstrated, after extraction from both producer and non-producer lines. we propose to designate the antigen as hupna. it can be detected by a human anti-ebna ...197773523
selective stimulation and differentiation of early antigens in lymphoblastoid cell lines producing epstein-barr-like viruses.comparisons of early antigens (ea) of ebv-related lymphotropic herpes virus of old world primates have proved difficult to accomplish because antigen levels are low in infected cells and the ratio of virus capsid antigen to ea is generally high. to overcome these difficulties, we have developed a procedure which combines antigen stimulation with inhibition of late virus functions and results in the selective stimulation of ea. using this procedure, we have examined the ea of ebv, herpesvirus pap ...197882609
evidence for antigenic distinctness of the epstein--barr virus-determined nuclear antigen and the herpesvirus papio-determined nuclear antigen.previous serological evidence indicated that epstein--barr virus-determined nuclear antigen (ebna) has cross reactive components with the herpesvirus papio-determined nuclear antigen (hupna) and, in addition, it has distinct components of its own, not present in hupna. in the present study, this hypothesis was studied by absorption experiments. absorption of anti-ebna positive human sera with ebna abolished both anti-ebna and anti-hupna reactivity. absorption with hupna removed all anti-hupna re ...197988257
herpesvirus papio: state and properties of intracellular viral dna in baboon lymphoblastoid cell lines.herpesvirus papio (hvp) is an indigenous b-lymphotropic virus of baboons (papio sp.) present in latent form in baboon lymphoblastoid cell lines. it shares cross-reacting viral capsid and early antigens with the epstein-barr virus (ebv), and hvp dna and ebv dna show partial sequence homology. ebv-specific complementary rna was employed here as a probe to investigate the physical state of the hvp dna component in baboon lymphoblastoid cells after fractionation of cellular dna by density gradient c ...1979113352
surface adhesive properties of baboon lymphocyte and derived lymphoblastoid cell lines.cell surface adhesiveness of lymphocytes isolated from the peripheral blood of papio hamadryas monkeys was compared to the adhesiveness of lymphoblastoid cell lines derived by transformation of these lymphocytes with herpesvirus papio. for the quantitative comparison of the cell surface adhesive properties, a recently developed latex particle adherence assay was utilized. it was found that cell surface adhesiveness of the suspension lines was substantially lower than that of their precursor lymp ...1979119650
transformation of lymphocytes by herpesvirus papio.cotton-topped (ct) or white-lipped (wl) marmoset lymphocytes were transformed in vitro with herpesvirus papio (hvp) into permanently growing lymphoblastoid cell lines (lcl). five of 9 hvp-transformed ct cell lines contained cells with antigens reacting with antibodies to epstein-barr virus (ebv) capsid antigen (vca) and/or to ebv-induced early antigens (ea). none of 12 wl lcl revealed such antigen-producing cells. cells from both groups of cultures failed to react with antibodies to the ebv-spec ...1977197025
susceptibility of marmosets to epstein-barr virus-like baboon herpesviruses.epstein-barr virus (ebv)-like herpeviruses, strains of herpesvirus papio (hvp), have recently been isolated from lymphomatous (hvp-l) and from normal baboons (hvp-n). both hvp isolates infect some species of marmosets, hvp-l inoculated adult animals develop a mild to severe and sometimes fatal lymphoproliferative disease whereas newborn marmosets of the same species do not develop a measureable disease after inoculation with hvp-l. hvp-n infects adult marmosets but does not cause disease.1978205854
antigen-inducing ability of herpesvirus papio in human and baboon lymphoma lines, compared to epstein-barr virus.herpesvirus papio(hvp)-carrying baboon lymphoblastoid lines do not express a nuclear antigen like the epstein-barr virus(ebv)-determined nuclear antigen (ebna), as judged by in situ anticomplement fluorescence staining, although the carry multiple viral genomes and, in the case of producerlines, early antigen (ea) and viral capsid antigen (vca) that cross-react with the corresponding human ebv-determined antigens. to test whether the lack of in situ nuclear antigen expression is a property innat ...1978210135
serological reactivities of human and baboon sera against ebna and herpesvirus papio-determined nuclear antigen (hupna). 1978213288
antibody responses to herpesvirus papio antigens in baboons with epstein-barr virus-related herpesvirus, termed herpesvirus papio (hvp), was isolated from baboons (papio hamadryas) at the institute of experimental pathology and therapy, sukhumi, ussr, where there is a continuing outbreak of lymphoma. in the present study sera from diseased baboons and from age- and sex-matched control animals were examined for antibodies to hvp antigens. results showed that animals with lymphoid disease had antibodies to hvp virus capsid, early, soluble, and nuclear antige ...1979216640
herpesvirus papio: state of viral dna in baboon lymphoblastoid cell lines.baboon lymphoblastoid cell lines (lcl) transformed with herpesvirus papio were evaluated for the state of hvp dna in the cells by caesium chloride density-gradient centrifugation and dna-crna filter hybridization. five hvp producer cultures and one non-producer culture were studied and non-integrated and integrated hvp dna were detected in all producer cultures whereas only integrated hvp dna could be demonstrated in the non-producer lcl. non-integrated hvp dna had a density of 1.715--1.716 g/cm ...1978221325
detection of a nuclear antigen in herpesvirus ateles-carrying marmoset lines by the acid-fixed nuclear binding vitro binding of a herpesvirus ateles (hva)-associated soluble antigen to amphibian erythrocyte nuclei was demonstrated by the acid-fixed nuclear binding technique in combination with anticomplement immunofluorescence. incubation of concentrated salt-extracted soluble antigens derived from hva-carrying marmoset lines with methanol/acetic acid-fixed erythrocytes of frogs and salamanders resulted in a brilliant nuclear fluorescence after exposure to a live virus-boostered, anti-hvs-positive squ ...1979221913
sites of sequence variability in epstein-barr virus dna from different sources.the intracellular epstein-barr virus (ebv) dna present in virus-transformed cells was partly purified from 23 cell lines or biopsies of burkitt lymphoma, nasopharyngeal carcinoma, infectious mononucleosis, or healthy carrier origin. such dna was cleaved in fragments (a-k) of molecular weights between 1 x 10(6) and 30 x 10(6) with restriction enzyme ecori, and these fragments were analyzed by standard methods involving agarose gel electrophoresis, transfer to nitrocellulose filters, and hybridiza ...1979223159
a review of herpesvirus papio, a b-lymphotropic virus of baboons related to ebv. 1979228899
prevalence of antibodies to soluble antigen of epstein-barr virus-producing cells (p3hr-1) in the sera of hamadryas baboons of the high lymphoma incidence stock.soluble antigen of p3hr-1 cells (sa-p3hr-1) was identified in indirect double immunodiffusion enhanced with tannic acid using serum of a nasopharyngeal carcinoma patient containing high-titer antibodies to epstein-barr virus (ebv) antigens. sa-p3hr-1 was nonidentical to soluble antigen of raji cells. human and baboon sera containing antibodies to all the known antigen of ebv and hvp respectively were anti-sa-3hr-1-positive. human and baboon sera containing antibodies to all the known antigens of ...1979231520
generation of macaque b lymphoblastoid cell lines with simian epstein-barr-like viruses: transformation procedure, characterization of the cell lines and occurrence of simian foamy virus.two simian epstein-barr-like viruses, a rhesus epstein-barr virus and herpesvirus papio, were used to transform b cells from rhesus or cynomolgus macaques. the resulting cell lines exhibited predominantly a b lymphocyte phenotype and expressed epstein-barr virus antigens. the majority of b lymphoblastoid cell lines from macaques, which were seropositive for simian foamy virus, developed giant cells in culture. the cytopathic agent was identified as a foamy virus and was transmissible to human em ...19921331148
baboon t cell lymphomas expressing the b cell-associated surface proteins cd40 and bgp95.papio hamadryas baboons in the sukhumi colony develop enzootic outbreaks of malignant lymphomas with an incidence of about 1.5% per year among adults of the high-risk stock. we investigated the surface phenotypes of cells from normal and lymphomatous animals using antibodies against human lymphocyte antigens. we found that more than 80% of the lymphomas that developed during the last 3 years were characterized histologically to be of the peripheral t cell type. generally, the lymphomatous cells ...19921383259
establishment and characterization of macaca fascicularis lymphoblastoid cell lines.a panel of cynomolgus macaque lymphoblastoid cell lines (lcl) was established by transforming peripheral blood mononuclear cells (pbmc) with herpesvirus papio (hvp), and selected lines were examined by flow cytometry. results indicate that hvp-transformed macaque lcl are phenotypically heterogeneous and resemble human epstein-barr virus (ebv)-transformed lcl in the abundant expression of major histocompatibility complex (mhc) class i and class ii molecules. at least some lines are of b cell orig ...19921602455
eap, a highly conserved cellular protein associated with epstein-barr virus small rnas (ebers).human b lymphocytes latently infected with epstein-barr virus (ebv) synthesize very large amounts (5 x 10(6)/cell) of two small nuclear rnas called ebers (epstein-barr encoded rnas). these rnas are of unknown function and, like many rna polymerase iii (pol iii) transcripts, bind the la autoantigen. we have discovered that the ebers also associate with a second highly abundant host-encoded protein designated eap (eber associated protein). human eap is a small (14,777 dalton, 128 amino acid) polyp ...19911846807
analysis of the epstein-barr virus origin of plasmid replication (orip) reveals an area of nucleosome sparing that spans the 3' dyad.the epstein-barr virus orip, when examined with the use of in vitro nucleosome reconstitution, was found to exhibit a partial sparing of nucleosome assembly within the 3' dyad. analysis of the homologous region containing the origin of replication of herpesvirus papio disclosed that sparing of nucleosome assembly was conserved between these two related viruses.19892555570
seroepizootiology of the herpesvirus papio (hvp) infection in healthy baboons (papio hamadryas) of high- and low-lymphoma risk populations.seroepizootiology of herpesvirus papio (hvp) infection was studied in three groups of healthy hamadryas baboons (papio hamadryas): the main sukhumi (high-lymphoma) stock, forest sukhumi (lymphoma-free) stock and newly imported wild animals. the prevalence to hvp infection, as judged by anti-vca-hvp positivity, was approximately the same in both sukhumi stocks (86% and 90% respectively) and it was significantly lower in the pooled group of newly imported baboons. it is interesting that prevalence ...19852988993
geographical prevalence of two types of epstein-barr virus.the jijoye ebv strain is characterized by a substitution of 1.8 kb in the c-terminal part of the ebna 2 gene compared to b95-8 or m-aba virus. this made it possible to construct hybridization probes specific for m-aba (type a) and jijoye viruses (type b), which have been used to type the ebv genomes in 38 spontaneously established cell lines. type a is more prevalent being found in 31 of 38 cases; type b virus was found in five cell lines (jijoye, ly 67, qimr-gor, bl 16, and bl 29); and two cell ...19863019008
herpesvirus papio contains a plasmid origin of replication that acts in cis interspecies with an epstein-barr virus trans-acting function.herpesvirus papio (hvp) and epstein-barr virus (ebv) are closely related biologically and biochemically; lymphoblastoid cells infected with either virus contain episomal viral dna. the putative origin of replication for ebv plasmids (orip) has been assigned to a 1,790-base-pair fragment (cis) in the short unique region of the genome which requires a viral function supplied in trans from elsewhere in the genome (j. yates, n. warren, d. reisman, and b. sugden, proc. natl. acad. sci. usa 81:3806-38 ...19863023667
leukocyte migration inhibition detects cross-reacting antigens between cells transformed by epstein-barr virus (ebv) and ebv-like simian viruses.the leukocyte migration inhibition (lmi) technique was used to measure the t cell-mediated immune response of epstein-barr-virus (ebv)-seropositive human donors to antigens associated with b cell lines of simian origin, transformed by simian ebv-like viruses, herpesvirus papio (hvp), h. pan, h. gorilla and h. pongo. extracts of cell lines carrying three of the four simian viruses (from gorilla, chimpanzee and orangutan) induced a positive lmi response, whereas lines carrying baboon-derived hvp w ...19863034820
collinear relationships of herpesvirus papio dna to epstein--barr virus dna. 19816259833
herpesvirus papio dna is similar in organization to epstein-barr virus dna.ecori, hindii, sali, nd xbai restriction endonuclease maps of herpesvirus papio (hvpapio) dna were derived by determining the fragment sizes and the linkage relationships between fragments generated by the different enzymes. the data indicate that hvpapio dna has a single molecular arrangement which is similar to that of epstein-barr virus dna. the size of the dna was 110 x 10(6) to 114 x 10(6) daltons. restriction fragments from both ends varied in the number of repeats of a 4 x 10(5)-dalton se ...19816261014
linkage map of the fragments of herpesvirus papio dna.herpesvirus papio (hvp), an epstein-barr-like virus, causes lymphoblastoid disease in baboons. the physical map of hvp dna was constructed for the fragments produced by cleavage of hvp dna with restriction endonucleases ecori, hindiii, sali, and pvui, which produced 12, 12, 10, and 4 fragments, respectively. the total molecular size of hvp dna was calculated as close to 110 megadaltons. the following methods were used for construction of the map; (i) fragments near the ends of hvp dna were ident ...19816261015
colinearity between the dnas of epstein-barr virus and herpesvirus papio.epstein barr virus (ebv) and herpesvirus papio (hvpapio) dnas share a common format and 40% homology. labeled cloned fragments of ebv dna were hybridized to blots of xbai, ecori, hindiii, and sali fragments of hvpapio dna. ebv fragments which mapped from 2 x 10(6) to 54 x 10(6) and from 59 x 10(6) to 106 x 10(6) daltons hybridized to fragments at identical map positions in hvpapio dna. regions of nonhomology were demonstrated at 0 x 10(6) to 2 x 10(6), 54 x 10(6) to 59 10(6), and 106 x 10(6) to ...19816261021
comparative studies of herpesvirus papio (baboon herpesvirus) dna and epstein-barr virus epstein-barr virus (ebv)-like herpesvirus has been isolated from a baboon cell line (594s/f9) by induction with the tumour promoter 12-o-tetradecanoyl-phorbol-13-acetate (tpa). the herpesvirus papio (hvp) dna was mixed with b95 ebv dna and was characterized by sedimentation in neutral glycerol gradient as 55s dna, with a buoyant density of approx. 1.718 g/ml after equilibrium centrifugation in caesium chloride. dna-dna reassociation kinetics between b95 ebv and hvp dna showed that hvp dna sha ...19806262436
immunofluorescent staining of nuclear antigen in lymphoid cells transformed by herpesvirus papio (hvp).an improved fixation method for antigen detection in lymphoblastoid cells is described. herpesvirus papio nuclear antigen (hupna) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (acif). antibody to hupna was detected in many human sera containing antibodies to epstein-barr virus capsid and nuclear antigen (ebna). rheumatoid arthritis sera showed a high incidence of both anti-ebna and anti-hupna antibodies.19816270210
dna-polymerase induced by herpesvirus papio (hvp) in cells of lymphoblastoid cultures derived from lymphomatous baboons. report v.a new dna-polymerase was found in the cells of suspension lymphoblastoid cultures which produce lymphotropic baboon herpesvirus (hvp). this enzyme was isolated in a partially purified form. some of its properties vary from those of other cellular dna-polymerases. hvp-induced dna-polymerase has a molecule weight of 160,000 and sedimentation coefficient of about 8 s. the enzyme is resistant to high salt concentration and n-ethylmaleimide, but it is very sensitive to phosphonoacetate. it effectivel ...19816276214
simple repeat sequence in epstein-barr virus dna is transcribed in latent and productive infections.the bamhi k region of epstein-barr virus dna is transcribed in latently infected cells from burkitt tumors and in growth-transformed b-lymphocytes latently infected with epstein-barr virus. we determined the nucleotide sequence of a 1,153-base pair hinfi fragment in bamhi fragment k from the b95-8 epstein-barr virus isolate. the fragment contains a remarkable 708-base pair simple sequence repeat array, designated ir3, which is composed of only three nucleotide triplet elements: ggg, gca, and gga ...19826292493
functional complementation between epstein-barr virus and herpesvirus papio.the possibility of complementation between herpesvirus papio (hvp) and epstein-barr virus (ebv) was investigated. strain 594s-f9 of hvp, unlike strain p3hr-1 of ebv, is not capable of inducing virus antigen synthesis in the ebv genome-carrying, nonproducer lymphoma cell line raji. the effects of dual infection with these viruses were studied. with untreated viruses, the percentage of cells positive for viral antigens was equal to or slightly less than that in cultures infected with p3hr-1 virus ...19836298145
enzyme-linked immunosorbent assay for detection of antibodies to epstein-barr virus antigens.enzyme-linked immunosorbent assay (elisa) was standardized for measurement of antibody activity of reference human and baboon (papio hamadryas) sera to soluble epstein-barr virus (ebv) antigens. a comparison with the immunofluorescent (if) method showed that elisa detects antibody specifically and sensitivity. in elisa, herpesvirus papio (hvp) nuclear antigen (hupna) positive baboon serum reacted with ebv nuclear antigen (ebna), as a further indication of the antigenic similarity between hvp and ...19836318497
5' coding and regulatory region sequence divergence with conserved function of the epstein-barr virus lmp2a homolog in herpesvirus papio.b-lymphotropic herpesviruses naturally infecting old world primates share biologic, epidemiologic, pathogenic, and molecular features with the human pathogen epstein-barr virus (ebv). these related gammaherpesviruses have colinear genomes with considerable nucleotide homology. the replicative cycle genes share a high degree of homology across species, whereas the transformation-associated ebv latent genes appear to be much more divergent. for example, the ebv bamhi nhet fragment, which encodes a ...19957494314
domains of the epstein-barr virus nuclear antigen 2 (ebna2) involved in the transactivation of the latent membrane protein 1 and the ebna cp promoters.the epstein-barr virus (ebv) nuclear antigen 2 (ebna2) is one of the first ebv-encoded gene products expressed after infection of primary b lymphocytes. ebna2 is essential for the growth-transforming potential of the virus and it functions as a transcriptional activator of a set of viral and cellular genes. sequence-specific dna-binding by ebna2 has not been demonstrated but the molecule is targeted to specific dna regions by a cellular protein, rbp-j kappa, which recognizes the gtgggaa sequence ...19957595374
herpesvirus papio 2, an sa8-like alpha-herpesvirus of baboons.several sa8 isolates obtained from baboons were compared to the prototype sa8 herpesvirus of african green monkeys. sds-page and restriction enzyme analyses revealed definite differences between green monkey and baboon isolates. dna and amino acid sequences of the gb, gd and gj glycoprotein genes exhibited substantial differences in variable regions. for the gb and gd, the amount of amino acid substitutions between sa8 and the baboon viruses was comparable to levels observed between analogous ge ...19957733825
contribution of conserved amino acids in mediating the interaction between ebna2 and cbf1/rbpjk.the epstein-barr virus ebna2 protein is a transcriptional activator that achieves promoter specificity through interaction with the cellular dna-binding protein cbf1/rbpjk. within the amino acid 252-to-425 ebna2 domain that targets cbf1/rbpjk lie three amino acid clusters, conserved regions (cr) 5, 6, and 7, that are retained in the epstein-barr virus type a and type b and herpesvirus papio proteins. to further define the important features of the targeting domain, we constructed ebna2 polypepti ...19957853539
the lytic origin of herpesvirus papio is highly homologous to epstein-barr virus ori-lyt: evolutionary conservation of transcriptional activation and replication signals.herpesvirus papio (hvp) is a b-lymphotropic baboon virus with an estimated 40% homology to epstein-barr virus (ebv). we have cloned and sequenced ori-lyt of herpesvirus papio and found a striking degree of nucleotide homology (89%) with ori-lyt of ebv. transcriptional elements form an integral part of ebv ori-lyt. the promoter and enhancer domains of ebv ori-lyt are conserved in herpesvirus papio. the ebv ori-lyt promoter contains four binding sites for the ebv lytic cycle transactivator zta, an ...19938389916
comparison of the ebna1 proteins of epstein-barr virus and herpesvirus papio in sequence and function.the ebna1 protein of epstein-barr virus (ebv) supports replication and maintenance of the circularized viral chromosome in cells that are latently infected. we have isolated, sequenced, and functionally characterized the ebna1 gene of herpesvirus papio (hvp), an ebv-like virus that infects baboons. the amino acid sequences of ebna1 of hvp and ebv are 56% identical, if the difference in the length of the glycine and alanine containing repetitive region, which is much shorter for hvp ebna1, is omi ...19968806482
comparative analysis identifies conserved tumor necrosis factor receptor-associated factor 3 binding sites in the human and simian epstein-barr virus oncogene lmp1.nonhuman primates are naturally infected with a b-lymphotropic herpesvirus closely related to epstein-barr virus (ebv). these simian ebv share considerable genetic, biologic, and epidemiologic features with human ebv, including virus-induced tumorigenesis. however, latent, transformation-associated viral genes demonstrate marked sequence divergence among species despite the conserved functions. we have cloned the latent membrane protein 1 (lmp1) homologs from the simian ebv naturally infecting b ...19968892903
anti-major histocompatibility complex antibody responses to simian b cells do not protect macaques against sivmac infection.macaques have been protected against infection with human cell-grown sivmac by immunization with antigens encoded by the human major histocompatibility complex (mhc). here, we investigated the efficacy of alloimmunization with simian b cells expressing high levels of mhc class i and class ii molecules to confer protection against systemic challenge with simian-grown sivmac. eight rhesus macaques were vaccinated with glutaraldehyde-fixed and beta-propiolactone-inactivated herpesvirus papio-transf ...19979223408
prevalence of herpesvirus papio 2 in baboons and identification of immunogenic viral polypeptides.the prevalence of herpesvirus papio 2 (hvp2) in several groups of captive and wild-caught baboons was determined by detection of anti-hvp2 antibodies in 133 sera of adult baboons. over 90% of newly imported (wild-caught) adult olive baboons (papio anubis) from kenya and chacma baboons (p. ursinus) from south africa were found to have anti-hvp2 titers. similarly, approximately 85% of captive breeding colony baboons (p. anubis and p. cynocephalus) were seropositive for hvp2. infected animals were ...19979241626
normal immunologic response to a neoantigen, bacteriophage phix-174, in baboons with long-term lymphohematopoietic reconstitution from highly purified cd34+ lin- allogeneic marrow cells.the cd34 antigen is thought to be expressed by hematopoietic stem cells in adult humans and nonhuman primates. we present data that baboons transplanted with highly purified allogeneic cd34+ marrow cells devoid of detectable mature and immature t and b lymphocytes and myeloid cells, isolated from sex-mismatched mixed lymphocyte culture (mlc) nonreactive siblings, have maintained stable lymphohematopoietic engraftment with donor cells for greater than 4.9, greater than 6.0, and 5.0 years. cytogen ...19979269791
shedding and transmission of baboon herpesvirus papio 2 (hvp2) in a breeding colony.baboons in a captive breeding colony were monitored twice a year, and new additions were screened on arrival for shedding of herpesvirus papio 2 (hvp2) and serologic reactivity to the agent. for 128 individual animals tested over a period of 1.5 years, shedding of infective virus was detected in 13 of 342 swab specimens (3.8%), each of these incidents representing shedding by a different animal. among long-term colony animals, infective virus was recovered on only two occasions (5 of 236 swab sp ...19989517885
transcriptional activation signals found in the epstein-barr virus (ebv) latency c promoter are conserved in the latency c promoter sequences from baboon and rhesus monkey ebv-like lymphocryptoviruses (cercopithicine herpesviruses 12 and 15).the epstein-barr virus (ebv) ebna2 protein is a transcriptional activator that controls viral latent gene expression and is essential for ebv-driven b-cell immortalization. ebna2 is expressed from the viral c promoter (cp) and regulates its own expression by activating cp through interaction with the cellular dna binding protein cbf1. through regulation of cp and ebna2 expression, ebv controls the pattern of latent protein expression and the type of latency established. to gain further insight i ...19999847397
mechanisms that regulate epstein-barr virus ebna-1 gene transcription during restricted latency are conserved among lymphocryptoviruses of old world primates.epstein-barr virus (ebv), the only known human lymphocryptovirus (lcv), displays a remarkable degree of genetic and biologic identity to lcvs that infect old world primates. within their natural hosts, infection by these viruses recapitulates many key aspects of ebv infection, including the establishment of long-term latency within b lymphocytes, and is therefore a potentially valuable animal model of ebv infection. however, it is unclear whether these lcvs have adopted or maintained the same me ...19999971778
development of a competitive elisa for detection of primates infected with monkey b virus (herpesvirus simiae).two competitive elisas (c-elisas) are described that allow detection of antibodies against monkey b virus (bv, cercopithecine herpesvirus 1). the assays utilize monoclonal antibodies (mabs) directed against the bv glycoprotein b (gb). two of these mabs specifically recognize bv gb while a third mab also reacts with the gb homologues of other primate alpha-herpesviruses (herpes simplexvirus-1, hsv-1: hsv-2; simian agent-8, sa8; and herpesvirus papio-2, hvp2). a c-elisa using the single cross-reac ...199910029325
herpesvirus papio 2: alternative antigen for use in monkey b virus diagnostic assays.serologic testing for antibody to monkey b virus (bv) in macaque sera is problematic due to the biohazardous nature of bv and bv antigens. herpesvirus papio 2 (hvp2), a herpesvirus of baboons, is more closely related genetically and antigenically to bv than is human herpes simplex virus 1 (hsv1). the potential for use of hvp2 relative to hsv1 as an alternative test antigen for detection of anti-bv antibody in macaque sera was assessed.199910638495
epidemiology of herpesvirus papio infection in a large captive baboon colony: similarities to epstein-barr virus infection in humans.the epidemiology of herpesvirus papio, a lymphocryptovirus similar to epstein-barr virus (ebv), was studied in a captive colony of >1900 baboons. herpesvirus papio igg antibody titers were measured by ifa. in total, 438 specimens from 296 baboons were assessed, including 116 serial specimens from 52 juveniles and 6 infants studied monthly for 1 year following birth and at age 18 months. maternally derived antibody reached a nadir at 4 months of age. about 75% of animals at 12 months of age and > ...200010762578
herpesvirus papio encodes a functional homologue of the epstein-barr virus apoptosis suppressor, bhrf1.the human tumour virus epstein-barr virus (ebv) encodes a 17 kda protein, bhrf1, which is a member of the bcl:-2 family and has been shown to suppress apoptosis. the role of this gene in the life-cycle of ebv has not been fully elucidated. in order to identify motifs conserved in herpesviruses and possibly shed light on its function we isolated a bhrf1 homologue from herpesvirus papio (cercopithecine herpesvirus-12) a closely related gammaherpesvirus of baboons. the gene, hvpbhrf1, also encodes ...200010859386
fatal lymphoproliferative disease associated with a novel gammaherpesvirus in a captive population of common marmosets.callitrichids (marmosets and tamarins) are extremely susceptible to experimental tumor induction by herpesviruses native to other primate species. a colony of common marmosets developed a syndrome of weight loss, inappetence, diarrhea, and in several animals, palpable abdominal masses.200010987671
amino acids of epstein-barr virus nuclear antigen 3a essential for repression of jkappa-mediated transcription and their evolutionary conservation.epstein-barr virus (ebv) nuclear antigen 3a (ebna-3a) is essential for virus-mediated immortalization of b lymphocytes in vitro and is believed to regulate transcription of cellular and/or viral genes. one known mechanism of regulation is through its interaction with the cellular transcription factor jkappa. this interaction downregulates transcription mediated by ebna-2 and jkappa. to identify the amino acids that play a role in this interaction, we have generated mutant ebna-3a proteins. a mut ...200111119577
bhrf1 is highly conserved in primate virus analogues of epstein-barr virus.the epstein-barr virus (ebv) protein bhrf1 (bamhi rightward reading frame 1) was the first viral member of the bcl-2 family of apoptosis-regulating proteins described. in vitro studies imply that bhrf1 is dispensable for virus-induced cellular transformation and virus replication. however, in contrast to several essential viral genes that show divergence outwith their functional domains, sequence data from a wide range of ebv isolates show there is striking conservation of the bhrf1 gene. contra ...200111223721
close but distinct regions of human herpesvirus 8 latency-associated nuclear antigen 1 are responsible for nuclear targeting and binding to human mitotic chromosomes.human herpesvirus 8 is associated with all forms of kaposi's sarcoma, aids-associated body cavity-based lymphomas, and some forms of multicentric castleman's disease. herpesvirus 8, like other gammaherpesviruses, can establish a latent infection in which viral genomes are stably maintained as multiple episomes. the latent nuclear antigen (lana or lnai) may play an essential role in the stable maintenance of latent episomes, notably by interacting concomitantly with the viral genomes and the meta ...200111264383
an animal model for human ebv-associated hemophagocytic syndrome: herpesvirus papio frequently induces fatal lymphoproliferative disorders with hemophagocytic syndrome in rabbits.epstein-barr virus-associated hemophagocytic syndrome (ebv-ahs) is often associated with fatal infectious mononucleosis. however, the animal model for ebv-ahs has not been developed. we reported the first animal model for ebv-ahs using rabbits infected with ebv-related herpesvirus of baboon (hvp). eleven of 13 (85%) rabbits inoculated intravenously with hvp-producing cells developed fatal lymphoproliferative disorders (lpd) between 22 and 105 days after inoculation. lpd was also accompanied by h ...200111290571
simian homologues of human herpesvirus 8.gamma-herpesviruses can be found in most primates including old world an new world monkeys. the gamma-herpesvirinae are grouped into two classes: lymphocryptoviruses (gamma1) and rhadinoviruses (gamma2). the lymphocryptoviruses include epstein-barr virus, lymphocryptovirus of rhesus monkeys, and herpesvirus papio of baboons. rhadinoviruses that infect new world monkeys include herpesvirus saimiri, whose natural host is the squirrel monkey, and herpesvirus ateles, which infects spider monkeys. rh ...200111313010
alphaherpesvirus antigen quantitation to optimize the diagnosis of herpes b virus infection.standardized, quantified virus antigen stocks are essential for dependable quality control of diagnostic assays. five simple, rapid and economical direct enzyme linked immunoassays (delisa) were developed to standardize and optimize antigen from five major cross-reacting alphaherpesviruses: herpes b virus, herpesvirus papio 2, langur monkey herpesvirus, herpes simplex virus-1 and herpes simplex virus-2. each delisa relied on pools of convalescent sera from rhesus monkeys, baboons, langurs and hu ...200211906729
one-step pcr to distinguish b virus from related primate adding betaine to the pcr mixture, we previously established a pcr method to amplify a dna segment of the glycoprotein g gene of b virus (bv) derived from a rhesus macaque. we have found that dna of other bv strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our pcr assay. under the same conditions no product was obtained with dna of simian agent 8 of green monkeys and herpesvirus papio 2 of baboons, or the human herpes simplex viruses types 1 ...200211986284
characterization of an epstein-barr virus-related gammaherpesvirus from common marmoset (callithrix jacchus).a gammaherpesvirus related to epstein-barr virus (ebv; human herpesvirus 4) infects otherwise healthy common marmosets (callithrix jacchus). long-term culture of common marmoset peripheral blood lymphocytes resulted in outgrowth of spontaneously immortalized lymphoblastoid cell lines, primarily of b cell lineage. electron microscopy of cells and supernatants showed herpesvirus particles. there were high rates of serological cross-reactivity to other herpesviruses (68-86%), but with very low geom ...200212075080
serological evidence of alpha herpesvirus infection in sooty between sooty mangabeys (sms) and a pigtailed macaque prompted the serological screening of sms for evidence of infection with b virus. serological tests detected sm antibodies that reacted with b virus polypeptides. additional testing was performed with sera from sms with no previous contact with macaques. results from these tests indicated that 56% (33/59) of the sms had antibodies that reacted with b virus and sa8. sm antibodies also reacted with herpesvirus papio 2 and to a lesser ex ...200212190852
comparative pathology of infections with baboon and african green monkey alpha-herpesviruses in mice.the comparative pathology of herpesvirus papio 2 (hvp2) of baboons and sa8 virus of african green monkeys relative to that of herpes simplex virus (hsv1) of man was investigated in young adult mice inoculated intramuscularly and observed for 21 days. the 50% infectious dose (id(50)) for hvp2 was approximately 10(2.0) plaque-forming units (pfu), while the id(50) for hsv1 and sa8 was 10(2.5) and 10(3.8), respectively. there were marked differences in the ability of these three viruses to invade th ...200212354526
animal in vivo models of ebv-associated lymphoproliferative diseases: special references to rabbit models.animal models of human ebv-associated diseases are essential to elucidate the pathogenesis of ebv-associated diseases. here we review those previous models using ebv or ebv-like herpesviruses and describe the details on our two newly-developed rabbit models of lymphoproliferative diseases (lpd) induced by simian ebv-like viruses. the first is cynomolgus-ebv-induced t-cell lymphomas in rabbits inoculated intravenously (77-90%) and orally (82-89%) during 2-5 months. ebv-dna was detected in periphe ...200212371154
herpesvirus papio 2 encodes a virion host shutoff function.infection of baboons with herpesvirus papio 2 (hvp-2) produces a disease that is similar to human infection with herpes simplex viruses (hsv). molecular characterization of hvp-2 has demonstrated that the virion contains a factor which rapidly shuts off host cell protein synthesis after infection. reduction of host cell protein synthesis occurs in parallel with the degradation of mrna species. a homolog of the hsv virion host shutoff (vhs) gene was identified by southern and dna sequence analysi ...200212490401
the genome of herpesvirus papio 2 is closely related to the genomes of human herpes simplex viruses.infection of baboons (papio species) with herpesvirus papio 2 (hvp-2) produces a disease that is clinically similar to herpes simplex virus (hsv-1 and hsv-2) infection of humans. the development of a primate model of simplexvirus infection based on hvp-2 would provide a powerful resource to study virus biology and test vaccine strategies. in order to characterize the molecular biology of hvp-2 and justify further development of this model system we have constructed a physical map of the hvp-2 ge ...200312771408
the spacing between adjacent binding sites in the family of repeats affects the functions of epstein-barr nuclear antigen 1 in transcription activation and stable plasmid maintenance.epstein-barr virus (ebv) and the closely related herpesvirus papio (hvp) are stably replicated as episomes in proliferating latently infected cells. maintenance and partitioning of these viral plasmids requires a viral sequence in cis, termed the family of repeats (fr), that is bound by a viral protein, epstein-barr nuclear antigen 1 (ebna1). upon binding fr, ebna1 maintains viral genomes in proliferating cells and activates transcription from viral promoters required for immortalization. fr fro ...200312842617
therapeutic trials for a rabbit model of ebv-associated hemophagocytic syndrome (hps): effects of vidarabine or chop, and development of herpesvirus papio (hvp)-negative lymphomas surrounded by hvp-infected lymphoproliferative disease.epstein-barr virus-associated hemophagocytic syndrome (ebv-ahs), which is often associated with fatal infectious mononucleosis or t-cell lymphoproliferative diseases (lpd), is a distinct disease characterized by high mortality. treatment of patients with ebv-ahs has proved challenging. to develop some therapeutic interventions for ebv-ahs, we examined the effectiveness of an antiviral agent (vidarabine) or chemotherapy (chop), using a rabbit model for ebv-ahs. fourteen untreated rabbits were ino ...200312973684
pathogenicity of different baboon herpesvirus papio 2 isolates is characterized by either extreme neurovirulence or complete comparisons of the pathogenicity of simian alphaherpesviruses in mice, two isolates of the baboon virus hvp2 were nearly as lethal as monkey b virus, a biological safety level 4 agent (j. w. ritchey, k. a. ealey, m. payton, and r. eberle, j. comp. pathol. 127:150-161, 2002). to confirm these results, mice were inoculated intramuscularly with 10(5) pfu of hvp2 isolates obtained from different baboon subspecies and primate centers. some of the hvp2 isolates (6 of 13) caused paralysis and death ...200314512523
use of herpesvirus papio 2 as an alternative antigen in immunoblotting assay for b virus diagnosis.herpesvirus papio 2 (hvp2), which infects baboons, is much more closely related genetically and antigenically to monkey b virus (bv) than to human herpes simplex virus 1(hsv1) and other related herpes viruses. the usefulness of hvp2 as an alternative test antigen in immunoblotting assays to detect bv-antibody in macaque monkey sera was assessed. six hvp2 proteins reacted with bv-positive sera in immunoblotting. no specific bands could be detected with bv-negative sera. these results show the use ...200415187363
herpesvirus pan encodes a functional homologue of bhrf1, the epstein-barr virus v-bcl-2.epstein-barr virus (ebv) latently infects about 90% of the human population and is associated with benign and malignant diseases of lymphoid and epithelial origin. bhrf1, an early lytic cycle antigen, is an apoptosis suppressing member of the bcl-2 family. in vitro studies imply that bhrf1 is dispensable for both virus replication and transformation. however, the fact that bhrf1 is highly conserved not only in all ebv isolates studied to date but also in the analogous viruses herpesvirus papio a ...200515691372
validation of an enzyme-linked immunosorbent assay kit using herpesvirus papio 2 (hvp2) antigen for detection of herpesvirus simiae (b virus) infection in rhesus monkeys.serologic testing for antibody to monkey b virus (bv) in macaque sera is problematic due to the biohazardous nature of bv antigens. herpesvirus papio 2 (hvp2), a herpesvirus of baboons, is nonpathogenic to humans and is genetically and antigenically more closely related to bv than is human herpes simplex virus 1. this paper describes the results of our in-house laboratory that compared a bv antigen-based enzyme-linked immunosorbent assay (elisa) by commercial testing laboratory and an hvp2-based ...200516089172
experimental infection of baboons (papio cynocephalus anubis) with apathogenic and neurovirulent subtypes of herpesvirus papio 2.cercopithecine herpesvirus 16 (herpesvirus papio 2; hvp2) is an alpha-herpesvirus of baboons (papio spp.) that generally causes minimal to inapparent disease in the natural host species. hvp2 is very closely related genetically and antigenically to cercopithecine herpesvirus 1 (monkey b virus; bv) of macaques, which is well known for its extreme lethality in nonmacaque species including humans. preliminary evidence suggests that a mouse model of hvp2 would be an excellent tool for studying zoono ...200516270898
the complete genome sequence of herpesvirus papio 2 (cercopithecine herpesvirus 16) shows evidence of recombination events among various progenitor herpesviruses.we have sequenced the entire genome of herpesvirus papio 2 (hvp-2; cercopithecine herpesvirus 16) strain x313, a baboon herpesvirus with close homology to other primate alphaherpesviruses, such as sa8, monkey b virus, and herpes simplex virus (hsv) type 1 and type 2. the genome of hvp-2 is 156,487 bp in length, with an overall gc content of 76.5%. the genome organization is identical to that of the other members of the genus simplexvirus, with a long and a short unique region, each bordered by i ...200616414998
neuropathogenesis of herpesvirus papio 2 in mice parallels infection with cercopithecine herpesvirus 1 (b virus) in humans.cercopithecine herpesvirus 1 (monkey b virus; bv) produces extremely severe and usually fatal infections when transmitted from macaque monkeys to humans. cercopithecine herpesvirus 16 (herpesvirus papio 2; hvp2) is very closely related to bv, yet cases of human hvp2 infection are unknown. however, following intramuscular inoculation of mice, hvp2 rapidly invades the peripheral nervous system and ascends the central nervous system (cns) resulting in death, very much like human bv infections. in t ...200616432011
a naturally occurring fatal case of herpesvirus papio 2 pneumonia in an infant baboon (papio hamadryas anubis).here we describe the unusual finding of herpesvirus pneumonia in a 7-d-old infant baboon (papio hamadryas anubis). this animal had been separated from its dam the morning of its birth and was being hand-reared for inclusion in a specific pathogen-free colony. the baboon was presented for anorexia and depression of 2 d duration. physical examination revealed a slightly decreased body temperature, lethargy, and dyspnea. the baboon was placed on a warm-water blanket and was given amoxicillin-clavul ...200616539338
herpesvirus papio 2 (hvp2): sequence analysis of the unique short (us) region. 200616604454
primary mouse dermal fibroblast cell cultures as an in vitro model system for the differential pathogenicity of cross-species herpesvirus papio 2 infections.infection of mice with herpesvirus papio 2 (hvp2) parallels zoonotic monkey b virus infections. a major benefit of the hvp2/mouse model is the existence of two hvp2 subtypes: hvp2nv rapidly invades and destroys the cns while hvp2ap produces no clinical signs and mild histopathological lesions. however, in the natural baboon host, no difference in pathogenicity of hvp2 subtypes is evident. primary dermal fibroblast cells were evaluated as a model system for defining virus-host interactions that i ...200717122896
emergence of anti-red blood cell antibodies triggers red cell phagocytosis by activated macrophages in a rabbit model of epstein-barr virus-associated hemophagocytic syndrome.hemophagocytic syndrome (hps) is a fatal complication frequently associated with viral infections. in childhood hps, epstein-barr virus (ebv) is the major causative agent, and red blood cells (rbcs) are predominantly phagocytosed by macrophages. to investigate the mechanism of rbc phagocytosis triggered by ebv infection, we adopted a rabbit model of ebv-associated hps previously established by using herpesvirus papio (hvp). the kinetics of virus-host interaction was studied. using flow cytometry ...200717456768
naturally transmitted herpesvirus papio-2 infection in a black and white colobus monkey.a 6.5-year-old female eastern black and white colobus monkey (colobus guereza) was evaluated after acute onset of ataxia and inappetence.200718081530
pathology of the male baboon (papio spp.) urogenital system.comprehensive reports on male baboon urogenital pathology are not available.200818194219
type i ifn response to papiine herpesvirus 2 (herpesvirus papio 2; hvp2) determines neuropathogenicity in mice.isolates of baboon alpha-herpesvirus papiine herpesvirus 2 (hvp2) exhibit one of two distinct phenotypes in mice: extremely neurovirulent or apathogenic. previous studies implicated the type i interferon (ifn) response as being a major factor in controlling infection by apathogenic isolates. to further investigate the possibility that the host ifn-beta response underlies the pathogenicity of the two hvp2 subtypes, the susceptibility of mice lacking the ifn-beta receptor (ifnar(-/-)) to infection ...200919215952
efficacy of peroxisome proliferator activated receptor agonist in the treatment of virus-associated haemophagocytic syndrome in a rabbit model.virus-associated haemophagocytic syndrome (vahs) is a fatal complication of viral infections, such as epstein-barr virus and h5n1 influenza, that results from macrophage activation and pro inflammatory cytokine injuries. the high comorbidity and mortality of current therapy urgently demands an ideal agent based on vahs pathogenesis. peroxisome proliferator activated receptor (ppar) agonists, regulators of metabolic syndrome, can exhibit immunomodulatory effects on macrophage activation and cytok ...201020167993
induction of encephalitis in rhesus monkeys infused with lymphocryptovirus-infected b-cells presenting mog(34-56) peptide.the overlapping epidemiology of multiple sclerosis (ms) and epstein-barr virus (ebv), the increased risk to develop ms after infectious mononucleosis (im) and the localization of ebv-infected b-cells within the ms brain suggest a causal link between ebv and ms. however, the underlying mechanism is unknown. we hypothesize that ebv-infected b-cells are capable of eliciting a central nervous system (cns) targeting autoimmune reaction. to test this hypothesis we have developed a novel experimental m ...201323977076
papiine herpesvirus 2 as a predictive model for drug sensitivity of macacine herpesvirus 1 (monkey b virus).monkey b virus (macacine herpesvirus 1; bv) is endemic in macaques. bv (a bsl4 agent) is the primary zoonotic concern for persons working with macaques in research, and human bv infections frequently are fatal. we assessed the use of a bsl2 baboon herpesvirus (papiine herpesvirus 1; hvp2) for predicting the drug sensitivity of bv by comparing the sensitivity of the 2 viruses to 12 antiherpetic drugs. plaque reduction assays showed that 4 drugs (hbpg, bvdu, pfa, and brdu) were ineffective against ...201425402179
detection systems for antibody responses against herpes b virus.herpes b virus (bv) infection is highly prevalent among adult asian macaques and rarely causes severe disease in infected animals. in contrast, bv infection of humans can induce fatal encephalitis in the absence of treatment. therefore, the development of diagnostic tests for specific and sensitive detection of antibodies against bv is an important task. the cross-reactivity of antibodies against bv with related simplex viruses of other primates may afford an opportunity to obtain sensitive dete ...201732110687
mapping ebna-1 domains involved in binding to metaphase chromosomes.the epstein-barr virus (ebv) genome can persist in dividing human b cells as multicopy circular episomes. viral episomes replicate in synchrony with host cell dna and are maintained at a relatively constant copy number for a long time. only two viral elements, the replication origin orip and the ebna-1 protein, are required for the persistence of viral genomes during latency. ebna-1 activates orip during the s phase and may also contribute to the partition and/or retention of viral genomes durin ...199910196336
a potential proline-rich motif upstream of the immunoreceptor tyrosine-based activation motif in bovine leukemia virus gp30, epstein-barr virus lmp2a, herpesvirus papio lmp2a, and african horsesickness virus vp7. 19968661376
ebna-2 of herpesvirus papio diverges significantly from the type a and type b ebna-2 proteins of epstein-barr virus but retains an efficient transactivation domain with a conserved hydrophobic motif.ebna-2 contributes to the establishment of epstein-barr virus (ebv) latency in b cells and to the resultant alterations in b-cell growth pattern by up-regulating expression from specific viral and cellular promoters. we have taken a comparative approach toward characterizing functional domains within ebna-2. to this end, we have cloned and sequenced the ebna-2 gene from the closely related baboon virus herpesvirus papio (hvp). all human ebv isolates have either a type a or type b ebna-2 gene. ho ...19938388484
the cellular rna-binding protein eap recognizes a conserved stem-loop in the epstein-barr virus small rna eber 1.eap (eber-associated protein) is an abundant, 15-kda cellular rna-binding protein which associates with certain herpesvirus small rnas. we have raised polyclonal anti-eap antibodies against a glutathione s-transferase-eap fusion protein. analysis of the rna precipitated by these antibodies from epstein-barr virus (ebv)- or herpesvirus papio (hvp)-infected cells shows that > 95% of eber 1 (ebv-encoded rna 1) and the majority of hvp 1 (an hvp small rna homologous to eber 1) are associated with eap ...19938380232
dna of herpesvirus pan, a third member of the epstein-barr virus-herpesvirus papio group.the dna of herpesvirus pan, a primate b-lymphotropic herpesvirus, shares about 40% well-conserved sequence relatedness with epstein-barr virus (ebv) and herpesvirus papio dnas. labeled cloned fragments from the ebv recombinant dna library were cross hybridized to blots of ecori, xbai, and bamhi restriction endonuclease fragments of herpesvirus pan dna to identify and map homologous sequences in the herpesvirus pan genome. regions of colinear homology were demonstrated between 6 x 10(6) daltons a ...19826284982
isolation and characterization of the genes for two small rnas of herpesvirus papio and their comparison with epstein-barr virus-encoded eber rnas.genes for the epstein-barr virus-encoded rnas (ebers), two low-molecular-weight rnas encoded by the human gammaherpesvirus epstein-barr virus (ebv), hybridize to two small rnas in a baboon cell line that contains a similar virus, herpesvirus papio (hvp). the genes for the hvp rnas (hvp-1 and hvp-2) are located together in the small unique region at the left end of the viral genome and are transcribed by rna polymerase iii in a rightward direction, similar to the ebers. there is significant simil ...19882839701
production of baboon (papio hamadryas) monoclonal antibodies by herpesvirus papio immortalized baboon lymph node cells.baboon monoclonal antibodies specific for monomorphic determinants of baboon apolipoprotein b were produced from in vivo primed lymph node cells immortalized by herpesvirus papio (hvp). two cell lines from the immortalized cells were readily stabilized and grew rapidly. since epstein-barr virus (ebv) cannot be used to immortalize baboon or macaque cells, hvp expands the potential sources of monoclonal antibodies to include baboons, and is the immortalizing agent of choice for macaques as well.19902303734
plasmid origin of replication of herpesvirus papio: dna sequence and enhancer function.herpesvirus papio (hvp) is a lymphotropic virus of baboons which is related to epstein-barr virus (ebv) and produces latent infection. the nucleotide sequence of the 5,775-base-pair (bp) ecori k fragment of hvp, which has previously been shown to confer the ability to replicate autonomously, has been determined. within this dna fragment is a region which bears structural and sequence similarity to the ori-p region of ebv. the hvp ori-p region has a 10- by 26-bp tandem array which is related to t ...19902159548
rabbit model for human ebv-associated hemophagocytic syndrome (hps): sequential autopsy analysis and characterization of il-2-dependent cell lines established from herpesvirus papio-induced fatal rabbit lymphoproliferative diseases with hps.epstein-barr virus-associated hemophagocytic syndrome (ebv-ahs) is often associated with fatal infectious mononucleosis or t-cell lymphoproliferative diseases (lpd). to elucidate the true nature of fatal lpd observed in herpesvirus papio (hvp)-induced rabbit hemophagocytosis, reactive or neoplastic, we analyzed sequential development of hvp-induced rabbit lpd and their cell lines. all of the seven japanese white rabbits inoculated intravenously with hvp died of fatal lpd 18 to 27 days after inoc ...200312707056
comparative analysis of the expression of the epstein-barr virus (ebv) anti-apoptotic gene bhrf1 in nasopharyngeal carcinoma and ebv-related lymphoid diseases.epstein-barr virus (ebv) has been identified in a wide range of neoplastic and non-neoplastic disorders. the ebv open reading frame bhrf1 encodes a protein with partial sequence and functional homology to the anti-apoptotic onco-protein bcl-2 and may therefore have a role in the proliferation of ebv positive cells. we have developed a rat monoclonal antibody against pbhrf1, which can detect bhrf1 in paraffin sections. while a number of mutant versions of bhrf1 were recognised, the monoclonal did ...200111505451
induction of target antigens and conversion to susceptible phenotype of nk-cell-resistant lymphoid cell line.two autologous herpesvirus papio producer lymphoid cell lines and one autologous non-producer line were compared for susceptibility to natural killer (nk) cell-mediated lysis. the non-producer cell line, 26cb-1, was more resistant to nk cell killing compared to one viral producer counterpart 13cb-1, but equally resistant when compared to another, 8cb-1. treatment with chemical agents that affect differentiation or activate the viral cycle, including n-butyrate, iudr, 5-azacytidine and tunicamyci ...19817309297
generation of a specific-pathogen-free baboon colony.we undertook establishing an spf baboon colony in response to requests from researchers. to enable the widest possible future use of spf baboons, our aim was to develop an spf colony of baboons (papio hamadryas anubis) free of 12 target viruses: 5 herpesviruses, 4 retroviruses, simian virus 40, measles, and monkeypox. infant baboons were removed from their mothers within 24 h of birth and nursery-reared. groups of 3 to 8 age-matched conspecifics were isolated in separate rooms for 1 y while unde ...201021205446
identifying sites bound by epstein-barr virus nuclear antigen 1 (ebna1) in the human genome: defining a position-weighted matrix to predict sites bound by ebna1 in viral genomes.we identified binding sites for epstein-barr virus (ebv) nuclear antigen 1 (ebna1) in the human genome using chromatin immunoprecipitation and microarrays. the sequences for these newly identified sites were used to generate a position-weighted matrix (pwm) for ebna1's dna-binding sites. this pwm helped identify additional dna-binding sites for ebna1 in the genomes of ebv, kaposi's sarcoma-associated herpesvirus, and cercopithecine herpesvirus 15 (cehv-15) (also called herpesvirus papio 15). in ...200919129441
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