Publications

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field evaluation of a phototoxic dye, phloxine b, against three species of fruit flies (diptera: tephritidae).the xanthene dye phloxine b (d&c red #28) bait was sprayed against fruit flies in mango orchards in 1996 and 1997. the flies used for testing were mexican fruit fly, anastrepha ludens (loew), west indian fruit fly, anastrepha obliqua (macquart), and mediterranean fruit fly, ceratitis capitata (wiedeman). results of the experiments indicate that the toxic efficacy of phloxine b against these fruit flies is as good as that of malathion-bait sprays. results also indicate that type of protein used w ...200111777044
effects of age, diet, female density, and the host resource on egg load in anastrepha ludens and anastrepha obliqua (diptera: tephritidae).oocyte counts, used as a measure of egg load, were compared among three different age groups (15, 30 and 45 days) of two polyphagous species of tephritid fruit flies, anastrepha ludens and anastrepha obliqua, which were exposed to varying conditions of diet (sucrose vs sucrose and protein), availability of oviposition substrate (present vs absent), adult female density (1, 2 and 4 females/cage), and semiochemical context (presence vs absence of male pheromones and fruit volatiles). in both speci ...200111472760
dietary self-selection and discrimination threshold in wild anastrepha obliqua females (diptera, tephritidae).experiments were performed on adult wild anastrepha obliqua females to (i) determine whether there is a critical period during the reproductive phase when the absence of protein in the diet impairs egg production; (ii) determine whether the females are able to self-select an optimal diet; and (iii) determine the discrimination threshold for protein in newly emerged females as well as in 10-day old females that had either been deprived of protein or not deprived. the results showed that protein i ...200112770190
hemocyte types and total and differential counts in unparasitized and parasitized anastrepha obliqua (diptera, tephritidae) larvae.the hemocyte types, in addition to total and differential hemocyte counts were studied in parasitized and unparasitized anastrepha obliqua larvae at the beginning and at the end of the third instar. in both developmental phases, in parasitized and unparasitized larvae, prohemocytes, plasmatocytes, granulocytes, adipohemocytes, spherulocytes and oenocytoids cells were observed. mitotic figures indicate prohemocytes as stem cells. prohemocytes, plasmatocytes and granulocytes are the most numerous ...200212659019
irradiation of anastrepha obliqua (diptera: tephritidae) revisited: optimizing sterility induction.the effects of irradiation doses increasing from 0 to 100 gy (1 gy is energy absorbed in j kg(-1) of irradiated material) on fertility, flight ability, survival, and sterile male mating performance were evaluated for mass-reared anastrepha obliqua (macquart). high sterility values (> 98.2%) for irradiated males were obtained for doses as low as 25 gy. egg hatch was inhibited for irradiated males crossed with irradiated females at a low dose of 20 gy. however, we estimated that to achieve 99.9% s ...200415154459
nonhost status of commercial persea americana 'hass' to anastrepha ludens, anastrepha obliqua, anastrepha serpentina, and anastrepha striata (diptera: tephritidae) in mexico.the objective of this study was to determine the host status in mexico of commercially cultivated and marketed avocado, persea americana (mill.), 'hass' to anastrepha ludens (loew), anastrepha obliqua (macquart), anastrepha serpentina (wiedemann), and anastrepha striata (schiner) (diptera: tephritidae). experiments in michoacán, mexico, were carried out in six orchards located at three altitudes above sea level during two times (august-october 2001 and april-june 2002). they included choice ('ha ...200415154448
the gene doublesex of the fruit fly anastrepha obliqua (diptera, tephritidae).the gene doublesex of anastrepha obliqua is composed of four instead of the usual six exons. it is transcribed in both sexes and its primary transcript undergoes sex-specific splicing, producing female dsx(f) and male dsx(m) proteins, which have in common the amino-terminal region but which differ at the carboxyl-terminal region.200516085699
sperm allocation and cost of mating in a tropical tephritid fruit fly.males that copulate repeatedly may suffer from reduced sperm stores. however, few studies have addressed sperm depletion from both the female and male perspective. here, we show that male anastrepha obliqua (diptera: tephritidae) do not ejaculate all available sperm and are left with mature sperm in the seminal vesicles even after copulating as often as three times in half a day. ejaculate size was not related to male mating history; time elapsed since the last mating, copulation duration, femal ...200616828793
a new potential attractant for anastrepha obliqua from spondias mombin fruits.nonirradiated males and females of anastrepha obliqua (macquart) were attracted to and landed more frequently on ripe fruits of spondias mombin l. than on artificial fruit in wind tunnel bioassays. porapak q volatile extracts of s. mombin were also attractive and elicited landing on artificial fruit for both sexes. combined gas chromatographic-electroantennographic detection (gc-ead) analysis of volatile extracts showed that nine volatile compounds elicited repeatable antennal responses from fem ...200616555133
nutritional balancing in fruit flies: performance of wild adult females of anastrepha obliqua (diptera: tephritidae) fed on single-food or food-pair treatments.the aim of the present study was to investigate the effects of different artificial diets, in which sucrose and brewer's yeast were offered separately (food-pair treatment) or mixed (single-food treatment), on the regulation of nutrient ingestion and the performance of wild adult females of anastrepha obliqua. the amount of diet consumed by females of a. obliqua increased as the concentration of yeast in the diet increased, revealing a phagostimulant effect of yeast. the amount of sucrose ingest ...200617078966
mating time of the west indian fruit fly anastrepha obliqua (macquart) (diptera: tephritidae) under laboratory conditions.allochronic reproductive isolation seems to be an important factor in speciation processes in tephritidae since specific mating times are a widespread feature of its species. the timing of matings of the west indian fruit fly, anastrepha obliqua (macquart) was investigated through group-focal observations, during ten days, under laboratory conditions. the number of observed matings and males exhibiting calling behavior varied significantly according to time of day. sexual activities seemed to be ...200717352082
factors influencing the release of volatiles in anastrepha obliqua males (diptera: tephritidae).during sexual signaling, males of anastrepha obliqua (macquart) form leks and attract females by producing sounds and releasing volatile compounds. the objective of this study was to investigate the influence time of time of day, age, irradiation, mating status, and the presence of host fruit on the release of volatile components by laboratory-reared a. obliqua males. a. obliqua males released four compounds identified by gas chromatography coupled with mass spectrometry (gc-ms) as (e,e)-alpha-f ...200818801252
[faunistic analysis of fruit flies (diptera: tephritidae) in the northern and northwestern regions of rio de janeiro state, brazil].this study aimed to characterize the fruit fly populations in three municipalities of the northern region and two municipalities of the northwestern region of rio de janeiro state, brazil, and to evaluate the similarity among their populations. a faunistic analysis was performed from the fruit fly specimens captured in plastic mcphail traps with an aqueous solution of hydrolyzed protein to 5% placed in orchards of guava (psidium guajava l.) and/or other fruits during 26 months. the total of 3,95 ...200818368244
male and female condition influence mating performance and sexual receptivity in two tropical fruit flies (diptera: tephritidae) with contrasting life histories.recent recognition of widespread polyandry in insects has generated considerable interest in understanding why females mate multiple times and in identifying factors that affect mating rate and inhibit female remating. however, little attention has been paid to understanding the question from both a female and male perspective, particularly with respect to factors that may simultaneously influence female remating rates. here, we report on a study aimed at ascertaining the possible interactive ef ...200919666025
field evaluation of potential fruit-derived lures for anastrepha obliqua (diptera: tephritidae).previous studies have shown that a nine-component blend (ethyl butyrate, isopropyl butyrate, hexan-1-ol, propyl butyrate, isobutyl butyrate, ethyl hexanoate, isopentyl butyrate, ethyl benzoate, and ethyl octanoate) isolated from spondias mombin l. (anacardiaceae) fruit are attractive to both sexes of west indian fruit fly, anastrepha obliqua (macquart) (diptera: tephritidae), in laboratory and field cage tests. in this study, we evaluated the effectiveness of traps baited with the nine-component ...200920069833
there is no magic fruit fly trap: multiple biological factors influence the response of adult anastrepha ludens and anastrepha obliqua (diptera: tephritidae) individuals to multilure traps baited with biolure or nulure.field-cage experiments were performed to determine the effectiveness of multilure traps (better world mfg inc., fresno, ca) baited with nulure (miller chemical and fertilizer corp., hanover, pa) or biolure (suterra llc, inc., bend, or) in capturing individually marked mexican fruit fly, anastrepha ludens (loew), and west indian fruit fly, anastrepha obliqua (macquart) (diptera: tephritidae), of both sexes. experimental treatments involved wild and laboratory-reared flies of varying ages (2-4 and ...200919253622
larval feeding substrate and species significantly influence the effect of a juvenile hormone analog on sexual development/performance in four tropical tephritid flies.the juvenile hormone (jh) analog methoprene reduces the amount of time it takes laboratory-reared anastrepha suspensa (caribbean fruit fly) males to reach sexual maturity by almost half. here, we examined if methoprene exerted a similar effect on four other tropical anastrepha species (anastrepha ludens, anastrepha obliqua, anastrepha serpentina and anastrepha striata) reared on natural hosts and exhibiting contrasting life histories. in the case of a. ludens, we worked with two populations that ...200919101560
demographic changes in anastrepha obliqua (diptera: tephritidae) throughout the laboratory colonization process.the demographic changes in anastrepha obliqua (macquart) (diptera: tephritidae) throughout laboratory colonization were characterized over the course of 11 generations. four significant changes were determined. the first change was a reduction in the preoviposition period from the g(p) to g(1). the second was that wild female flies had difficulty ovipositing in an artificial substrate, yielding the lowest fecundity rates observed throughout the experiment. the third significant change was a decr ...200919449633
application of feces extracts and synthetic analogues of the host marking pheromone of anastrepha ludens significantly reduces fruit infestation by a. obliqua in tropical plum and mango backyard orchards.we determined the efficacy of three potential oviposition deterrents in reducing fruit infestation by anastrepha obliqua in tropical plum and mango orchards. these were: (1) extracts of feces of mexican fruit fly, anastrepha ludens, known to contain the a. ludens host marking pheromone (hmp) and (2) two fully synthetic simplified analogues of the naturally occurring compound, which we have named desmethyl a. ludens hmp (dm-hmp) and anastrephamide. two applications of feces extracts 2 or 3 wk bef ...200920069857
response of anastrepha obliqua (diptera: tephritidae) to visual and chemical cues under seminatural conditions.the west indian fruit fly, anastrepha obliqua (macquart), is considered one of the most important pests of several fruit crops in latin america. studies on the sensorial ecology of a. obliqua may contribute to the improvement of a trapping system for monitoring this fruit fly species. in this study, we investigated the responses of both sexes of a. obliqua to different visual cues and to the combination of visual and chemical cues in field cages tests. both sexes were more attracted to lime-gree ...200919610408
diversity of anastrepha spp. (diptera: tephritidae) and associated braconid parasitoids from native and exotic hosts in southeastern bahia, brazil.we documented fruit fly-host associations and infestation rates over 5 yr in the state of bahia, brazil, by systematically collecting native and introduced fruits in backyard and commercial orchards, experimental stations, and patches of native vegetation. fruit were collected in multiple sites in the southern and southernmost regions of bahia. a total of 942.22 kg from 27 fruit species in 15 plant families was collected throughout this study. of these, 15 plant species from six families were in ...201022546440
chilled packing systems for fruit flies (diptera: tephritidae) in the sterile insect technique.we evaluated three packing systems (parc boxes, "gt" screen towers and "mx" screen towers) for the emergence and sexual maturation of sterile fruit flies, at three adult fly densities (1, 1.2 and 1.3 fly/cm²) and three food types. at the lowest density, results showed no significant differences in the longevity and flight ability of adult anastrepha ludens (loew) and anastrepha obliqua macquart among the three packing systems. higher densities resulted in a decrease in these parameters. in the e ...201020877998
grapefruit as a host for the west indian fruit fly (diptera: tephritidae).the most common hosts for the west indian fruit fly, anastrepha obliqua (macquart) (diptera: tephritidae) are fruit in the family anacardiaceae (mango [mangifera l.] and mombin [spondias l.] species). however, similar to many of the tropical fruit flies of major economic importance, this species attacks several other families of crop fruit, including annonaceae (cherimoya, annona cherimola mill.), myrtaceae (guava, psidium l.), oxalidaceae (carambola, averrhoa carambola l.), passifloraceae (gran ...201121404839
Anastrepha ludens and Anastrepha serpentina (Diptera: Tephritidae) do not infest Psidium guajava (Myrtaceae), but Anastrepha obliqua occasionally shares this resource with Anastrepha striata in nature.This study examined whether economically important fruit fly species Anastrepha ludens (Loew), Anastrepha serpentina (Wiedemann), and Anastrepha obliqua (Macquart) (Diptera: Tephritidae) may opportunistically exploit guavas, Psidium guajava L. (Myrtaceae), growing near preferred natural hosts. We collected 3,459 kg of guavas and 895 kg of other known host species [sour orange, Citrus aurantium L.; grapefruit, Citrus paradisi Macfadyen; mango, Mangifera indica L.; white sapote, Casimiroa edulis L ...201121882684
effect of rootstock on mango fruit susceptibility to infestation by anastrepha obliqua.the effect of the use of rootstock criollo on the susceptibility of manila mango fruit to infestation by anastrepha obliqua (macquart) was determined in the present investigation. growth, quality parameters (soluble solids content (%), citric acid content (%), firmness, monoterpene volatiles (3-carene, limonene, alpha-pinene, and beta-myrcene), main flavonoids content (milligrams/100 g fresh pulp), and larvicidal activity of methanolic extracts, as well as the degree of infestation during prehar ...201122299362
effect of temperature on pupa development and sexual maturity of laboratory anastrepha obliqua adults.the effect of four temperatures (18, 20, 25 and 30°c) on pupa development and sexual maturity of anastrepha obliqua adults was investigated under laboratory conditions. the results showed that the duration of the pupal stage decreased with an increase in temperature (29, 25, 13 and 12 days, respectively), and maintaining the pupae at 18°c and 20°c results in a low percentage of pupation, pupa weight loss and lesser flying ability. however, it significantly favored sexual behavior, a higher propo ...201121473797
understanding long-term fruit fly (diptera: tephritidae) population dynamics: implications for areawide management.fruit flies (diptera: tephritidae) are devastating agricultural pests worldwide but studies on their long-term population dynamics are sparse. our aim was to determine the mechanisms driving long-term population dynamics as a prerequisite for ecologically based areawide pest management. the population density of three pestiferous anastrepha species [anastrepha ludens (loew), anastrepha obliqua (macquart), and anastrepha serpentina (wiedemann)] was determined in grapefruit (citrus x paradisi macf ...201222812118
phylogeography of anastrepha obliqua inferred with mtdna sequencing.anastrepha obliqua (macquart) (diptera: tephritidae), the west indian fruit fly, is a frugivorous pest that occasionally finds its way to commercial growing areas outside its native distribution. it inhabits areas in mexico, central and south america, and the caribbean with occasional infestations having occurred in the southern tier states (california, florida, and texas) of the united states. this fly is associated with many plant species and is a major pest of mango and plum. we examine the g ...201223356081
mass rearing history and irradiation affect mating performance of the male fruit fly, anastrepha obliqua.as an initial step to improve the efficiency of the sterile insect technique applied to eradicate, suppress, and control wild anastrepha obliqua (macquart) (diptera: tephritidae) in mango producing areas of mexico, the effect of radiation dose and mass rearing history on male mating performance was examined. field cage tests in which both male and female laboratory flies were irradiated at different doses (0, 40, and 80 gy) were released with cohorts of wild flies of both sexes, revealing that b ...201222957485
species of anastrepha (diptera: tephritidae) captured in a guava orchard (psidium guajava l., myrtaceae) in boa vista, roraima, brazil.the guava fruit (psidium guajava) is among the most strongly affected by fruit flies in brazil. in the brazilian amazon, 11 species of anastrepha have been reported in guava orchards to date. this work aimed to identify the species of anastrepha present in a guava orchard in the municipality of boa vista, determine the species infesting the fruits, and identify any parasitoids present. two mcphail traps with food bait were installed and weekly collections were made between january and december 2 ...201324789406
juvenile hormone analog enhances calling behavior, mating success, and quantity of volatiles released by anastrepha obliqua (diptera: tephritidae).the application of a juvenile hormone analog, methoprene, to newly emerged adult males reduced the time required for sexual maturation and enhanced mating success in several species of tephritid fruit flies. in this work, we investigated the effect of topical methoprene application on west indian fruit fly, anastrepha obliqua (macquart), male calling, mating, and volatile release. males treated with topical methoprene exhibited sexual maturation and reproductive behavior 2 d earlier when compare ...201323575016
forest fragments as barriers to fruit fly dispersal: anastrepha (diptera: tephritidae) populations in orchards and adjacent forest fragments in puerto rico.mcphail-type traps baited with ammonium acetate and putrescine were used to monitor populations of anastrepha obliqua (macquart) and anastrepha suspensa (loew) in two orchards with hosts of these flies (mango, mangifera indica l., and carambola, averrhoa carambola l.), as well as in forest fragments bordering these orchards. contour maps were constructed to measure population distributions in and around orchards. our results indicate that anastrepha populations are focused around host fruit in b ...201323575019
transcriptome analysis of female reproductive tissues of anastrepha obliqua and molecular evolution of eggshell proteins in the fraterculus group.the investigation of cdna libraries has been an important tool for the identification of new genes in nonmodel species such as the fruit flies from the anastrepha fraterculus group. in the present study, we constructed a cdna library from the female reproductive tissues of anastrepha obliqua aiming to identify genes with high evolutionary rates. we sequenced 2304 clones obtained from the female reproductive tissues of a. obliqua flies. the expressed sequence tags generated a total of 816 unigene ...201324137793
susceptibility of 15 mango (sapindales: anacardiaceae) cultivars to the attack by anastrepha ludens and anastrepha obliqua (diptera: tephritidae) and the role of underdeveloped fruit as pest reservoirs: management implications.we evaluated the susceptibility of 15 mango cultivars to the attack of anastrepha ludens (loew) and anastrepha obliqua (macquart) (diptera: tephritidae), the main tephritid pests of this crop in mexico. in a field experiment, bagged fruit-bearing branches were exposed to gravid females of both fly species. infestation rates, developmental time, adult eclosion, and f1 adult longevity, fecundity, and fertility were recorded, ranking cultivars in terms of susceptibility to fly attack and developmen ...201424665723
the current and future potential geographic range of west indian fruit fly, anastrepha obliqua (diptera: tephritidae).the west indian fruit fly, anastrepha obliqua (macquart), is one of the most important pests throughout the americas. climex 3.0 and arcgis 9.3 were used to model the current and future potential geographical distribution of this pest. under current climatic conditions, a. obliqua is predicted to be able to establish throughout much of the tropics and subtropics, including not only north and south america, where it has been reported, but also southern asia, northeastern australia and sub-saharan ...201423956160
effect of multiple endogenous biological factors on the response of the tephritids anastrepha ludens and anastrepha obliqua (diptera: tephritidae) to multilure traps baited with biolure or nulure in mango orchards.the physiological state of an insect is likely the most important endogenous factor influencing resource-oriented behavior, and it varies considerably among individuals. trials were conducted in mango orchards to study the effect of multiple endogenous biological factors on the response of two fly species, anastrepha ludens (loew) and anastrepha obliqua maquart (diptera: tephritidae), to biolure and nulure baits. the biological factors of the two fly species that were tested were the following: ...201425026661
morphometric divergence in populations of anastrepha obliqua (diptera, tephritidae) from colombia and some neotropical locations.the west indian fruit fly, anastrepha obliqua, is one of seven species of quarantine importance of its genus and is one of the most economically important fruit fly pests in colombia. the taxonomic status of this species is a key issue for further implementation of any pest management program. several molecular studies have shown enough variability within anastrepha obliqua to suggest its taxonomic status could be revised; however, there are no morphological studies supporting this hypothesis. t ...201526798254
a new adult diet formulation for sterile males of anastrepha ludens and anastrepha obliqua (diptera: tephritidae).a new adult diet formulation was evaluated for sterile anastrepha ludens (loew) and anastrepha obliqua (macquart) males at the emergence and release facility of fruit flies in mexico. the formulation consists of hydrolyzed protein, sugar, juvenile hormone analogue methoprene, and water. the proportion of the ingredients between the solute (4% hydrolyzed protein and 96% sugar) and solvent (10% methoprene and 90% water) was 5:1. this new formulation was called the 1:24 formulation. the main object ...201526470310
assessing the risk of invasion by tephritid fruit flies: intraspecific divergence matters.widely distributed species often show strong phylogeographic structure, with lineages potentially adapted to different biotic and abiotic conditions. the success of an invasion process may thus depend on the intraspecific identity of the introduced propagules. however, pest risk analyses are usually performed without accounting for intraspecific diversity. in this study, we developed bioclimatic models using maxent and boosted regression trees approaches, to predict the potential distribution in ...201526274582
natural parasitism in fruit fly (diptera: tephritidae) populations in disturbed areas adjacent to commercial mango orchards in chiapas and veracruz, mexico.to determine the natural parasitism in fruit fly populations in disturbed areas adjacent to commercial mango orchards in the states of chiapas and veracruz, mexico, we recorded over one year the fruit fly-host associations, fly infestation, and parasitism rates in backyard orchards and patches of native vegetation. we also investigated the relationship between fruit size, level of larval infestation, and percent of parasitism, and attempted to determine the presence of superparasitism. the most ...201626850034
comparison of hydrolyzed protein baits and various grape juice products as attractants for anastrepha fruit flies (diptera: tephritidae).mexican fruit flies, anastrepha ludens (loew; diptera: tephritidae), have traditionally been trapped in citrus orchards in mexico using protein hydrolysates as bait. recently, ceratrap(®), an enzymatic hydrolyzed protein, has emerged as an effective lure for monitoring a. ludens at the orchard level and is currently being used by growers in the region of veracruz. several studies have revealed that grape juice is highly attractive to a. ludens, and recent work supports its potential use for regu ...201626396199
influence of methoprene on pheromone emission and sexual maturation of anastrepha obliqua (diptera: tephritidae) males.it has been demonstrated that the application of juvenile hormone analog, methoprene, reduces the time required for sexual maturation and enhances mating success in several species of tephritid fruit flies. this study examined the effect of different concentrations of methoprene incorporated into the diet of adult flies and distinct sugar:protein (s:p) ratios on sexual maturity and pheromone emission of anastrepha obliqua males. diets with 0.2 and 0.5% of methoprene accelerated sexual maturation ...201626797870
carambola cultivar, fruit ripeness, and damage by conspecific larvae influence the host-related behaviors of anastrepha obliqua (diptera: tephritidae).in this study, we investigated the influence of cultivar type, fruit ripeness, and damage by conspecific larvae on the attraction of anastrepha obliqua (macquart) (diptera: tephritidae) to and oviposition on carambola fruit (averroha carambola l.). the attraction of both sexes of a. obliqua to fruit of different quality was evaluated through cage experiments in the field, and the oviposition preferences of mated females were examined in laboratory tests. both sexes, mated or virgin, were more at ...201626411483
reference genes for accessing differential expression among developmental stages and analysis of differential expression of obp genes in anastrepha obliqua.the west indian fruit fly, anastrepha obliqua, is an important agricultural pest in the new world. the use of pesticide-free methods to control invasive species such as this reinforces the search for genes potentially useful in their genetic control. therefore, the study of chemosensory proteins involved with a range of responses to the chemical environment will help not only on the understanding of the species biology but may also help the development of environmentally friendly pest control st ...201626818909
resolution of inter and intra-species relationships of the west indian fruit fly anastrepha obliqua.the west indian fruit fly, anastrepha obliqua (diptera: tephritidae), is an economically important pest that inhabits areas of south and central america, mexico and the caribbean with occasional infestations in the southern united states. we examine intra-specific relationships within a. obliqua as well as interspecific relationships to other anastrepha species using a multi-locus data set comprising nine loci (seven nuclear, two mitochondrial) with 105 operational taxonomic units. the results b ...201627126185
comparative responses of anastrepha ludens and anastrepha obliqua (diptera: tephritidae) to the synthetic attractant biolure.the responses of wild and sterile anastrepha ludens (loew) and anastrepha obliqua (mcquart) fruit flies to the synthetic attractant biolure were determined by electroantennography (eag), in field cage tests using multilure traps, and by release-recapture field experiments using sterile flies. in eag bioassays, no differences were found between species, sexes, sterile and wild flies. there were only specific differences and interactions in dose responses. more a. ludens than a. obliqua individual ...201627473263
toward an automated identification of anastrepha fruit flies in the fraterculus group (diptera, tephritidae).in this study, we assess image analysis techniques as automatic identifiers of three anastrepha species of quarantine importance, anastrepha fraterculus (wiedemann), anastrepha obliqua (macquart), and anastrepha sororcula zucchi, based on wing and aculeus images. the right wing and aculeus of 100 individuals of each species were mounted on microscope slides, and images were captured with a stereomicroscope and light microscope. for wing image analysis, we used the color descriptor local color hi ...201627156899
wolbachia in guilds of anastrepha fruit flies (tephritidae) and parasitoid wasps (braconidae).the endosymbiont wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. aiming to determine if horizontal transmission might have occurred between anastrepha fruit flies and associated braconid wasps, infection by wolbachia was screened by amplification of a fragment of the wsp gene. eight species of the genus anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. th ...201627648768
patterns of inner chorion structure in anastrepha (diptera: tephritidae) eggs.the inner chorion structure of anastrepha eggs from 16 species of various infrageneric taxonomic groups is described by scanning and transmission electron microscopy. the layers of the chorion, the outer egg membrane, are structurally similar. furthermore, an additional trabecular layer (atl) that exists in some species, together with other characteristics, facilitates the recognition of four patterns of chorion structuring: pattern i, in which the atl layer is absent, is found in anastrepha ami ...201727847335
identifying anastrepha (diptera; tephritidae) species using dna barcodes.molecular identification of fruit flies in the genus anastrepha (diptera; tephritidae) is important to support plant pest exclusion, suppression, and outbreak eradication. morphological methods of identification of this economically important genus are often not sufficient to identify species when detected as immature life stages. dna barcoding a segment of the mitochondrial cytochrome oxidase i gene has been proposed as a method to identify pests in the genus. the identification process for the ...201729202187
experimental hybridization and reproductive isolation between two sympatric species of tephritid fruit flies in the anastrepha fraterculus species group.among tephritid fruit flies, hybridization has been found to produce local adaptation and speciation, and in the case of pest species, induce behavioral and ecological alterations that can adversely impact efficient pest management. the fraterculus species group within anastrepha (diptera: tephritidae), is a rapidly radiating aggregate, which includes cryptic species complexes, numerous sister species, and several pest species. molecular studies have highlighted the possibility of introgression ...201728586142
odorant-binding proteins expression patterns in recently diverged species of anastrepha fruit flies.we studied two species of closely related south american fruit flies, anastrepha fraterculus and anastrepha obliqua which, despite being able to interbreed, still show some ecological and reproductive differences. because part of these differences, such as host and mate preferences, may be related to olfactory perception, we focused our investigation on the differential expression of odorant-binding protein (obp) gene family, which participate in initial steps of the olfactory signal transductio ...201728526847
influence of pupation substrate on mass production and fitness of adult anastrepha obliqua macquart (diptera: tephritidae) for sterile insect technique application.tephritid mass-rearing systems require an artificial substrate for pupation. pupation substrate characteristics influence the quality of insects produced. coconut fiber, as an alternative to the conventional pupation substrate vermiculite, was evaluated for anastrepha obliqua macquart (diptera: tephritidae) pupation behavior (pupation patterns, distribution, respiration rate, and pupal weight) and adult fitness (adult eclosion time, flight ability, and male mating competitiveness). pupation perc ...201729045652
fitness of mass-reared males of anastrepha obliqua (diptera: tephritidae) resulting from mating competition tests in field cages.the sterile insect technique uses males that have been mass-reared in a controlled environment. the insects, once released in the field, must compete to mate. however, the mass-rearing condition supposes a loss of fitness that will be noticeable by wild females. to compare the fitness of wild males and mass-reared males, three competition settings were established. in setting 1, wild males, mass-reared males and wild females were released in field cages. in setting 2, wild females and wild males ...201729040620
benzalkonium chloride provides remarkable stability to liquid protein lures for trapping anastrepha obliqua (diptera: tephritidae).hydrolyzed protein lures are widely used to monitor fruit fly pests but are rapidly degraded by microbial activity and must be replaced frequently. to improve the stability of lures, the quaternary ammonium biocide, benzalkonium chloride (bc), was evaluated in mixtures with two hydrolyzed proteins commonly used to monitor anastrepha spp. the mean number of anastrepha obliqua adults captured during six consecutive weeks using captor + borax with the addition of 240 mg bc/liter, not renewed during ...201729029124
effect of resin ducts and sap content on infestation and development of immature stages of anastrepha obliqua and anastrepha ludens (diptera: tephritidae) in four mango (sapindales: anacardiaceae) cultivars.we determined the influence of resin ducts, sap content, and fruit physicochemical features of four mango cultivars (criollo, manila, ataulfo, and tommy atkins) on their susceptibility to the attack of the two most pestiferous fruit fly species infesting mangoes in mexico: anastrepha ludens (loew) and anastrepha obliqua (macquart). we performed three studies: 1) analysis of resin ducts in mango fruit exocarp to determine the density and area occupied by resin ducts in each mango cultivar, 2) ass ...201728073981
visibility and persistence of marker dyes and effect on the quality and mating competitiveness of mass-reared flies (diptera: tephritidae): anastrepha obliqua and bisexual and genetic sexing (tapachula-7) strains of a. ludens.fluorescent dyes are commonly used in the sterile insect technique (sit) for marking insects for a proper identification after recapture. however, the quality of the mark must be balanced against insect performance, because dyes can negatively affect some parameters of insect performance and reduce their effectiveness in control with the sit. we determined the visibility and persistence and the effect of dyes on the quality of anastrepha obliqua (macquart) and anastrepha ludens (loew) (bisexual ...201728549185
mass trapping is as effective as ground bait sprays for the control of anastrepha (diptera: tephritidae) fruit flies in mango orchards.anastrepha fruit flies are considered one of the main phytosanitary problems for the fresh fruit industry in the usa, caribbean islands and latin america. since 1994, the mexican government implemented the national fruit fly program using an area-wide integrated pest management approach. in this paper, we evaluated the effectiveness of mass trapping and compared it with ground gf-120 spraying against anastrepha obliqua and anastrepha ludens populations in mango cv ataulfo orchards.201728382747
sexual competitiveness, field survival, and dispersal of anastrepha obliqua (diptera: tephritidae) fruit flies irradiated at different doses.the sterile insect technique (sit) is used in area-wide pest management programs for establishing low pest prevalence and/or areas free of fruit flies (diptera: tephritidae). the aim of this technique is to induce high levels of sterility in the wild population, for this the released insects must have a high sexual competitiveness and field dispersal. however, radiation decreases these biological attributes that do not allow it to compete successfully with wild insects. in this study the sexual ...201829361120
evidence of adaptive evolution and relaxed constraints in sex-biased genes of south american and west indies fruit flies (diptera: tephritidae).several studies have demonstrated that genes differentially expressed between sexes (sex-biased genes) tend to evolve faster than unbiased genes, particularly in males. the reason for this accelerated evolution is not clear, but several explanations have involved adaptive and nonadaptive mechanisms. furthermore, the differences of sex-biased expression patterns of closely related species are also little explored out of drosophila. to address the evolutionary processes involved with sex-biased ex ...201829346618
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