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is the giant catfish, silurus glanis, a predator on man? 194521004969
[histochemical studies of oxidative enzymes in the gastric mucosa of sheat-fishes, frogs, tortoises and pigeons].oxyntic cells of gastric glands of the sheat-fish silurus glanis, frog rana temporaria, tortoise agrionemis horsfieldi and pigeon columba livia exhibit intensive reactions on dehydrogenases of lactate, alpha-ketoglutarate, glutamate, succinate, malate and glucose-6-phosphate which indicate high energy metabolism necessary for the production of hcl by these cells. cytoplasm of these cells is stained for the presence of nucleic acids, which may be associated with the other function of the oxyntic ...19751082694
[morphology of the endocrine portion of the teleost pancreas].endocrine component of the crucian (carassius carassius), carp (cyprinus carpio), tench (tinca tinca) and silurus (silurus glanis) pancreas is structurally organized in the form of pancreatic islets. gorbusha (oncorhynchus gorbuscha) has, besides the islets, some brockman's bodies. endocrine component of the pancreas of teleostei possesses a-, b-, d and acinar-islet cells "b". all types of cells are shoot-shaped and all have contacts with the capillaries. extrusion of the hormones from the endoc ...1978343755
oxygen transport and acid-base balance in the blood of the sheatfish, silurus glanis.oxygen binding and buffer properties of the blood of the sheatfish, silurus glanis, were investigated in vitro at 20 and 10 degrees c. the o2 binding curves were hyperbolic with p50 = 10.1 mm hg (20 degrees c, ph = 7.5) and 4.6 (10 degrees c, ph = 7.5). there was a very large bohr effect with an average delta log p 50/delta ph of - 1.14. at 20 degrees c this value tended to be higher than at 10 degrees c. as a consequence the apparent heat of oxygenation depended on ph. the mean value of delta h ...19816798660
morphological and biochemical studies on liver, kidney and gill of fishes affected by pesticides.effect of a herbicide, paraquat (1,1'-dimethyl-4,4'-bipyridilium-dichloride), the fungicide copper sulphate, and zinc chloride was studied on the histological structure of liver, kidney and gill of three fish species with different feeding habits, viz.: a herbivorous, silver carp (hypophthalmichthys molitrix); an omnivorous, common carp (cyprinus carpio l.) and a carnivorous, sheatfish (silurus glanis l.). the organs were studied electron microscopically after fixation according to karnovsky. th ...19836230560
partial characterization of transferrins of catfish (silurus glanis l.) and pike (esox lucius l.).basic composition and properties of isolated transferrins of silurus glanis and esox lucius have been compared. in transferrin of s. glanis carbohydrate is absent, but it is present in transferrin of e. lucius (2.5%). the n-terminal amino acid is alanine in both species. mol. wts are 68,400 (s. glanis) and 86,800 (e. lucius). transferrins of the two species are heterogeneous, but genetic polymorphism was not observed.19853995929
[the presence of enterovirus in species of shellfish and fish].enteroviruses were isolated from 12% of the digestive tract samples collected from anodonta cygnea (9.4%) and dreysena polymorpha (13.2%) shell species and from 8.4% of the branchiae sample from fish species rutilus rutilus, perca fluviatilis and silurus glanis. the isolated belonged to the following antigenic types: echo 2 and 7, coxsackie b 2, 4 and 6, and poliovirus 1 and 2.19902171625
comparative study of acetylcholine synthesis in organs of freshwater teleosts.the activity of acetylcholine (ach) synthesizing enzyme, choline acetyltransferase (chat), and the content of ach were determined in the brain, heart, red trunk muscle and midgut of 12 freshwater teleost species belonging to eight families: carp (cyprinus carpio), tench (tinca tinca), silver carp (hypophthalmichtys molitrix), bighead (hypophthalmichtys nobilis), wels (silurus glanis), cat fish (ictalurus nebulosus), eel (anguilla anguilla), rainbow trout (oncorhynchus mykiss), pike (esox lucius) ...199124214667
occurrence of pomphorhynchus laevis müller 1776 (acanthocephala) in silurus glanis (l.) from the river po.the sheatfish, silurus glanis (l.), from the terminal part of river po was examined for the presence of helminth parasites. of 182 s. glanis specimens, 95 (52.2%) were infected with the acanthocephalan pomphorhynchus laevis. mid-gut followed by fore-gut appeared to be the most infected portions of host alimentary canal. in 45 sheatfish of total length < or = 40 cm, specimens of p. laevis were found encapsulated in mesenteric and peritoneal tissues. a comparison between light and electron microsc ...19921339980
sphaerospora siluri n. sp. (myxosporea: sphaerosporidae) in the kidney of the sheatfish (silurus glanis).sphaerospora siluri sp. n. is a parasite of the sheatfish (silurus glanis) cultured in hungarian pond farms. spores and pseudoplasmodia of the species inhabit the lumen of the renal tubules, attaching loosely to the tubular epithelium. spores developing in monosporous pseudoplasmodia have an almost triangular shape.19938017237
steroidogenesis by ovaries and testes of the european catfish, the wels (silurus glanis), in vitro.testosterone, 3α,17-dihydroxy-5β-pregnen-20-one, 17,20β-dihydroxy-4-pregnen-3-one (17,20βp) and 5β-pregnane-3α,17,20β-triol were identified as the major metabolites of [(3)h] 17-hydroxyprogesterone in ovarian incubations of the european catfish silurus glanis. 17,20βp and the reduced triol were present only in ovaries from fish primed with carp hypophysial homogenate (chh) while testosterone yields were significantly higher in controls than in treated fish. 11-ketotestosterone, 11β-hydroxytestos ...199324214377
distribution of vasoactive intestinal peptide-like immunoreactivity in the taste organs of teleost fish and frog.using immunohistochemistry, vasoactive intestinal peptide (vip) was visualized in taste bud cells of the carp, cyprinus carpio, and the european catfish, silurus glanis, by means of light and electron microscopy. intracellular membrane systems, presumably smooth endoplasmic reticulum, of light (sensory) cells, but not of dark (supporting) cells and basal cells, were densely labelled with antibody. in the frog (four species: rana temporaria, r. ridibunda, r. arvalis, r. pipiens), taste bud cells ...19957775201
production of recombinant insulin-like growth factor-ii in the development of a radioimmunoassay in rainbow trout (oncorhynchus mykiss).rainbow trout (oncorhynchus mykiss) insulin-like growth factor-ii (igf-ii) cdna was amplified by reverse transcription-polymerase chain reaction and cloned into an expression vector (parhs3) for production by escherichia coli. recombinant rainbow trout igf-ii (rtigf-ii) was recovered from bacterial inclusion bodies, solubilized, and purified by gel filtration chromatography under reducing conditions. after refolding of the peptide by dialysis at basic ph, we obtained a final yield of 820 microgr ...19968930606
is the major capsid protein of iridoviruses a suitable target for the study of viral evolution?iridoviruses are large cytoplasmic dna viruses that are specific for different insect or vertebrate hosts. the major structural component of the non-enveloped icosahedral virus particles is the major capsid protein (mcp) which appears to be highly conserved among members of the family iridoviridae, phycodnaviridae, and african swine fever virus. the amino acid sequences of the known mcps were used in comparative analyses to elucidate the phylogenic relationships between different cytoplasmic dna ...19989562891
comparison of european systemic piscine and amphibian iridoviruses with epizootic haematopoietic necrosis virus and frog virus 3.iridovirus-like agents isolated from systemic infected fish (silurus glanis, sfir; ictalurus melas, cfir i, cfir ii, cfir iii) and from frogs (rana esculenta, reir) in europe, epizootic haematopoietic necrosis virus (ehnv) isolated in australia from redfin perch (perca fluviatilis), and frog virus 3 (fv 3) isolated from frogs (rana pipiens) in the usa were investigated by electron microscopy, polypeptide composition, immunofluorescence, restriction endonuclease digestion, southern-blot hybridiza ...19989719770
effects of dimerized lysozyme (klp-602) on the cellular and humoral defence mechanisms in sheatfish (silurus glanis): in vitro and in vivo study.this study examined the effects of the dimerized lysozyme (klp-602) on the immunocompetence cell activity in sheatfish (silurus glanis) and its influence in vivo on the non-specific defence mechanisms and protection against motile aeromonad septicaemia (mas). the in vitro study showed that the lysozyme dimer (klp-602), at concentrations between 5 and 50 micrograms/ml of medium significantly (p < 0.05) increased the respiratory burst activity and potential killing activity of pronephric macrophag ...199910478423
scolex morphology of the cestode silurotaenia siluri (batsch, 1786) (proteocephalidae: gangesiinae), a parasite of european wels (silurus glanis).the morphology of the scolex of the tapeworm silurotaenica siluri (batsch, 1786) (proteocephalidae: gangesiinae), a specific parasite of european wels (silurus glanis l.), was studied using light, scanning (sem), and transmission electron (tem) microscopy. the scolex of s. siluri is rather uniform in shape and size; it is globular, and has four prominent, anteriorly directed suckers and a spherical, muscular apical organ corresponding to the rostellum of cyclophyllidean cestodes. the rostellum-l ...19999950220
effect of the pesticide deltamethrin on the mauthner cells of lake balaton fish.deltamethrin, a synthetic pesticide [(s)alpha-cyano-3-phenoxybenzyl-(1r)-cis-3-(2.2-dibromovinyl)-2,2-dim ethylcyclopropane-carboxylate] used for extermination of mosquitoes on the shores of lake balaton, has been found to induce severe impairments of the nervous system of several lake balaton fish, such as carp (cyprinus carpio), goldfish (carassius auratus gibelis bloch), eel (anguilla anguilla) and wels (silurus glanis). it has been shown that deltamethrin, in a concentration of 1 microgram/l ...200010894124
rapid differentiation of australian, european and american ranaviruses based on variation in major capsid protein gene sequence.epizootic haematopoietic necrosis virus (ehnv), bohle iridovirus (biv) and wamena virus (wv) cause serious diseases in fish, amphibians and snakes, respectively but are restricted to australasia. european catfish virus (ecv) and sheatfish virus (esv) have caused epizootics in fish on farms in continental europe. currently there are no simple or readily available methods to distinguish these viruses, which are in the iridoviridae. they are culturally, morphologically and antigenically very simila ...200212030764
a new tapeworm, postgangesia inarmata n. sp. (eucestoda: proteocephalidea: gangesiinae), parasitic in silurus glanis (siluriformes) from iraq and some comments on the gangesiinae mola, 1929.we describe a new species of cestode belonging to postgangesia akhmerov, 1969 (proteocephalidea: gangesiinae) from the catfish silurus glanis linnaeus in iraq. this is the second species known from this genus, and a redescription of the type-species, p. orientalis akhmerov, 1969 (emend.), as well as a differential diagnosis are provided. the new species is characterised by the arrangement of its vitelline follicles, and various meristic and metrical characters. the systematic position of postgan ...200312878849
new records of alien monogeneans from lepomis gibbosus and silurus glanis in italy.the first geographical records of five alien monogeneans for italy is reported. four species have been detected from lepomis gibbosus (linnaeus, 1758): actinocleidus recurvatus mizelle et donahue, 1944, actinocleidus oculatus (mueller, 1934), urocleidus similis (mueller, 1936) and urocleidus dispar (mueller, 1936); one species from silurus glanis linnaeus, 1758: thaparocleidus vistulensis (siwak, 1932) n. comb.200315267103
the application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa.the viability of spermatozoa has been assessed using sybr 14 staining for dna of living cells and propidium iodide staining for dna of degenerate cells. this dual staining was performed on four fish species (siberian sturgeon, acipenser baerii; common carp, cyprinus carpio; tench, tinca tinca and wels, silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. ten phase contrast and epifluorescent images were recorded per sam ...200415566965
anatomy and morphometry of the olfactory organ of the wels silurus glanis l. (siluridae, pisces).the anatomical structure of the olfactory organs, nerve tracts and brain was described in silurus glanis. the changes connected with aging were considered. the olfactory lamellae are thin and tightly set in a rosette. in the 1 year old individuals there are 48...51 lamellae in a single rosette. this number increases gradually with age and in the 9...10 year old welses reaches 150. the surface area of the lamellae of a single rosette also indicates an increase: in the 1 year old specimen it equal ...2004524988
modulative influence of lysozyme dimer on defence mechanisms in the carp (cyprinus carpio) and european sheatfish (silurus glanis) after suppression induced by herbicide roundup.immunomodulation is a commonly used method of prophylaxis in humans and animals. lysozyme dimer (klp-602) was used at a dose of 50 ug/kg b.w. in order to correct the immunosuppression caused by the action of herbicide glyphosate (roundup- monsanto), which was used in a single bath for 10 minutes in a concentration of 100 mg/l of water. the investigations were carried out on 2 species of fish: the carp (cyprinus carpio l.) and european catfish (silurus glanis l.). herbicide glyphosate caused a de ...200415230544
arsenic speciation patterns in freshwater fish.muscle of 16 freshwater fish (9 different species belonging to 4 different families) was analysed for arsenic species using hplc separation (anion and cation exchange) followed by on-line uv-decomposition, hydride generation and afs detection. the main arsenic compounds found in the extracts were arsenobetaine (asb), which accounted for 92-100% of extractable arsenic in species of salmonids (salmo marmoratus, oncorhynchus mykiss, salmo trutta m. fario), and dimethylarsinic acid (dmaa), which acc ...200418969382
levels of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in fish species from kahramanmaras, turkey.the levels of organohalogenated contaminants, such as organochlorine pesticides (ocps), polychlorinated biphenyls (pcbs) and polybrominated diphenyl ethers (pbdes) were measured in four fish species (acanthobrama marmid (kalashpa), cyprinus carpio (carp), chondrostoma regium (nose-carp), and silurus glanis (wels)) from the sir dam lake, kahramanmaras, turkey. these species were selected for their characteristic feeding behaviour and their importance to local human fish consumption. ddts were the ...200515910967
accumulation of some metals in muscles of five fish species from lower nitra river.this paper reports the results of accumulated selected metals concentrations (fe, mn, zn, cu, ni, co, cr, pb, cd, hg and mehg) in the muscle of five common slovak fish species (chub-leuciscus cephalus, common carp-cyprinus carpio, prussian carp-carassius gibelio, roach-rutilus rutilus, and wels catfish-silurus glanis). furthermore, correlations among the selected metals and order of metal accumulation in the fish muscle were determined. the concentrations of metals (mg/kg wet weight basis) range ...200617000549
determination of cadmium and copper in fish samples from sir and menzelet dam lake kahramanmaraş, turkey.the cadmium and copper levels were determined in the total of 126 fish samples which belongs to five fish species collected from sir and menzelet dam lakes in kahramanmaraş province by atomic absorption spectrophotometer. the concentrations of heavy metals were expressed as ppm wet weight of tissue. the mean levels of cadmium and copper in muscle, liver and gill tissues of cyprinus carpiofrom the menzelet dam were found as 0.27, 0.91, 1.49 and 0.94, 1.2, 1.05, respectively. the mean levels of cd ...200616917713
the presence of microcystins and other cyanobacterial bioactive peptides in aquatic fauna collected from greek freshwaters.toxic bloom-forming cyanobacteria can cause animal death and adversely affect human health. blooms may contain microcystins (mcs), cyanobacterial heptapeptide hepatotoxins and other peptides such as anabaenopeptins and anabaenopeptilides. mcs have been shown to occur in various aquatic organisms including mussels, water snails, crustaceans and fish. muscle and viscera samples from eight species of fish (acipenser gueldenstaedtii, carassius auratus, carassius gibelio, cyprinus carpio, perca fluvi ...200616540185
detection of toxic effects of cd2+ on different fish species via liver cytochrome p450-dependent monooxygenase activities and ftir spectroscopy.the in vivo and in vitro effects of cd2+ and the cyp1a inductor beta-naphthoflavone(beta-nf) on the hepatic cytochrome p450 (cyt 450) monooxygenases were studied in silver carp (hypophthalmichtys molitrix v.), wels (silurus glanis l.), and carp (cyprinus carpio). in vivo treatment of carp with a high dose of cd2+ (10 mg kg(-1), for 3 days) caused a strong inhibition of 7-ethoxyresorufin-o-deethylase (erod) and a lower inhibition of 7-ethoxycoumarin-o-deethylase (ecod) activity. the low-dose cadm ...200616715285
ultrastructure of the secondary osmoregulatory canals in the scolex and neck region of silurotaenia siluri (batsch, 1786) (cestoda: proteocephalidae).the secondary osmoregulatory canals in the scolex and neck region of silurotaenia siluri, a parasite of the catfish silurus glanis (l.), terminate below the tegument basal plasma membrane. the basal plasma membrane of the osmoregulatory canal syncytium is in tight contact with the tegument basal plasma membrane.200616696435
heavy metal accumulation in water, sediments and fishes of nallihan bird paradise, turkey.the accumulation of some heavy metals such as pb, cd, cu and ni was determined in water sediment and fish samples (albumus escherichii, cyprinus carpio and silurus glanis) collected from nallihan bird paradise (nbp) and its vicinity (sariyar dam). the results showed that these metals are found widespread throughout the study area, but metal concentrations in the water samples are below the detection limits (bdl). pb, cd, cu and ni contamination were determined in sediments and in fish tissues (m ...200718380073
development of real-time pcr assays for the detection and differentiation of australian and european ranaviruses.serious systemic disease in fish and amphibians is associated with the ranaviruses, epizootic haematopoietic necrosis virus (ehnv) and bohle iridovirus (biv) in australia, and european sheatfish virus (esv) and european catfish virus (ecv) in europe. ehnv, esv and ecv are recognized causative agents of the oie (office international des epizooties) notifiable systemic necrotizing iridovirus syndrome and are currently identified by protein-based assays, none of which are able to rapidly identify t ...200717584440
heavy metal and trace elements in various fish samples from sir dam lake, kahramanmaraş, turkey.achanthobrama marmid (thorn-bream) (n:24), chondrostoma regium (nose-carp) (n:33) and silurus glanis (wels) (n:21), and cyprinus carpio (carp) (n:30) were collected from sir dam lake in kahramanmaraş province. the iron (fe), manganese (mn), cobalt (co), nickel (ni), and lead (pb) levels were determined in the total of 108 fish samples by atomic absorption spectrophotometer. the concentrations of heavy metals were expressed as mg kg(-1) wet weight of tissue. the mean fe and mn levels of muscle an ...200717057953
histopathological changes in the livers and kidneys of fish in sariyar reservoir, turkey.in this study, a total of 180 fish specimens (wels: silurus glanis-60; common carp: cyprinus carpio-60; bleak: alburnus escherichii-60) of ages between one and two were caught at three different stations in sariyar reservoir. the histological changes in the livers and kidneys of three different species of fish were detected microscopically and evaluated with quantitative analyses. also, organochlorine pesticide residues (ocp) have also been determined in the water and sediment samples and in the ...200721783764
[phenotype structure and its dynamics at different stages of the reproductive period of proteocephalus osculatus (cestoda: proteocephalidae)--a parasite of catfish (silurus glanis l.)].discrete variability of four p. osculatus characteristics descriptive of cestodes' major functional complexes: attachment and trophic-reproduction, was determined. phenotypic diversity of p. osculatus from 2 samples collected at different stages of the population reproductive period was analysed. unequal adaptation of phenotypes to the ambient conditions was hypothesised.200712916397
experimental infection and detection of aphanomyces invadans in european catfish, rainbow trout and european eel.european catfish silurus glanis, european eel anguilla anguilla and rainbow trout oncorhynchus mykiss were challenged by intramuscular injection of zoospores of aphanomyces invadans, the oomycete associated with epizootic ulcerative syndrome (eus). the tropical three-spot gourami trichogaster trichopterus is known to be highly susceptible and was used as a positive control. european catfish were highly susceptible and rainbow trout had moderate to low susceptibility, whereas eels appeared largel ...200819244971
effects of cu(2+) and pb(2+) on different fish species: liver cytochrome p450-dependent monooxygenase activities and ftir spectra.the effects of cu(2+)-sulfate and pb(2+)-acetate on carp (cyprinus carpio l.), silver carp (hypopthalmichtys molitrix v.) and wels (silurus glanis l.) were studied. the liver microsomal cyt p450 content, the erod, ecod and apnd monooxygenase activities were measured. in vivo treatment with 1 mg l(-1) cu(2+) significantly elevated the activities of these enzymes and cyt p450 content in silver carp livers. the high-dose cu(2+) treatment (10 mg l(-1)) on silver carp caused two-fold higher induction ...200818439880
[the "staircase" phenomenon in the myocardial activity of fishes and amphibians].studies have been made on chrono- and inotropic effects in the heart of the frog rana temporaria and fishes silurus glanis and cyprinus carpio during stationary and transitional stimulation. in contrast to data in the literature, no interspecific differences were found in changes of the amplitude of cardiac contractions induced by changes in rhythmic stimulation.200895845
trophic ecology of the pikeperch (sander lucioperca) in its introduced areas: a stable isotope approach in southwestern france.during the last decades, non-native predatory fish species have been largely introduced in european lakes and rivers, calling for detailed information on the trophic ecology of co-existing native and non-native predators. the present study describes the trophic ecology of the introduced pikeperch (sander lucioperca) in two southwestern french rivers, using stable isotope analysis. pikeperch could be categorized as a top-predator, and had a significantly higher trophic position (tp, mean+/-se=4.2 ...200919632657
ranavirus phylogeny and differentiation based on major capsid protein, dna polymerase and neurofilament triplet h1-like protein genes.in this study, we developed new methods for differentiation of ranaviruses based on polymerase chain reaction and restriction enzyme analysis of dna polymerase and neurofilament triplet h1-like (nf-h1) protein gene. using these methods, we were able to differentiate the 6 known ranaviruses--bohle iridovirus (biv), european catfish virus (ecv), epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), frog virus 3 (fv3) and singapore grouper iridovirus (sgiv)--with 3 less ch ...200919694168
susceptibility of pike esox lucius to a panel of ranavirus isolates.in order to study the pathogenicity of ranaviruses to a wild european freshwater fish species, pike esox lucius fry were challenged with the following ranavirus isolates: epizootic haematopoietic necrosis virus (ehnv), european sheatfish virus (esv), european catfish virus (ecv), pike-perch iridovirus (ppiv), new zealand eel virus (nzeelv) and frog virus 3 (fv3). the fry were infected using bath challenge at 12 and 22 degrees c. significant mortalities were observed at 12 degrees c for ehnv, esv ...200919402450
the in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout (oncorhynchus mykiss), european eel (anguilla anguilla) and wels catfish (silurus glanis).lactoferrin (lf) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. although fish are devoid of this protein and its cell receptors, lf effect on the immune mechanisms of fish has been demonstrated. the objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in thr ...201021077435
evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians.a rabbit polyclonal antibody (pab) raised against european catfish virus (ecv; isolated from black bullhead ameiurus melas in france) was produced and then evaluated using a panel of 9 ranavirus isolates collected from different lower vertebrate species originating from australia, north and south america, southeast asia, and europe. using ranavirus-infected epithelioma papillosum cyprini (epc) cell cultures, the specificity of the pab was determined by western blot, immunogold electron microscop ...201020481086
iridovirus infections in finfish - critical review with emphasis on ranaviruses.viruses in three genera of the family iridoviridae (iridoviruses) affect finfish. ranaviruses and megalocytiviruses are recently emerged pathogens. both cause severe systemic disease, occur globally and affect a diversity of hosts. in contrast, lymphocystiviruses cause superficial lesions and rarely cause economic loss. the ranavirus epizootic haematopoietic necrosis virus (ehnv) from australia was the first iridovirus to cause epizootic mortality in finfish. like other ranaviruses, it lacks hos ...201020050967
susceptibility of black bullhead ameiurus melas to a panel of ranavirus isolates.ranaviruses are considered a serious threat to lower vertebrates, including fish, amphibians and reptiles. however, epidemiological data on these agents are lacking, and further investigations are needed to understand the role of carriers and to update the list of susceptible hosts. we carried out various experimental infections under controlled conditions to contribute to the current knowledge on the susceptibility of black bullhead ameiurus melas to european catfish virus (ecv) and other ranav ...201020815324
quantitation of ranaviruses in cell culture and tissue samples.a quantitative real-time pcr (qpcr) based on a standard curve was developed for detection and quantitation of ranaviruses. the target gene for the qpcr was viral dna polymerase (dnapol). all ten ranavirus isolates studied (epizootic haematopoietic necrosis virus, ehnv; european catfish virus, ecv; european sheatfish virus, esv; frog virus 3, fv3; bohle iridovirus, biv; doctor fish virus, dfv; guppy virus 6, gv6; pike-perch iridovirus, ppiv; rana esculenta virus italy 282/i02, rev282/i02 and shor ...201021087639
comparative study of ranavirus isolates from cod (gadus morhua) and turbot (psetta maxima) with reference to other ranaviruses.two iridovirus isolates recovered from cod (gadus morhua) and turbot (psetta maxima) in denmark were examined in parallel with a panel of other ranaviruses including frog virus 3 (fv3), the reference strain for the genus ranavirus. the isolates were assessed according to their reactivity in immunofluoresent antibody tests (ifat) using both homologous and heterologous antisera and their amplification in pcr using primers targeting five genomic regions. the corresponding pcr fragments were sequenc ...201020552236
induction of diploid gynogenesis in wels catfish (silurus glanis) using uv-irradiated grass carp (ctenopharyngodon idella) sperm.this article describes the first successful induction of meiotic gynogenesis in wels catfish (silurus glanis) using heterologous sperm of grass carp (ctenopharyngodon idella) with dna inactivated by means of uv-irradiation at 4800 j/m(2). the cold shock at 6 degrees c (duration of 20 min) started in the nineth minute after fertilization was found as efficient in retaining the second polar body and producing meiotic diploids. successful induction of meiotic gynogenesis was confirmed by the micros ...201019722222
[role of aeromonas in the monitoring of a hydroecosystem in the volga-caspian area].the paper gives data obtained over many years on the quantity of bacteria of the genus aeromonas in the hydroecosystems of the delta of the volga and caspian sea and in commercial fishes (sturgeon (acipenseridae), pike perch (stizostedion), carp (cyprinus carpio), silurus (silurus glanis), etc.). it provides a detailed analysis of their seasonal trends and spatial distribution, markers of their pathogenicity, and antibiotic resistance. the findings suggest that aeromonas is a causative agent of ...201121842729
introduction and spread of non-native parasites with silurus glanis l. (teleostei: siluridae) in uk fisheries.despite growing concern of the ecological risks posed by the european catfish siluris glanis l. in freshwater fisheries, little information exists on the parasite fauna of this silurid catfish in britain. parasitological examinations of released s. glanis from four still-water fisheries in england revealed the presence of thaparocleidus vistulensis (siwak, 1932) and ergasilus sieboldi (nordmann, 1832), both non-native parasites, the latter known to be an important fish pathogen. this represents ...201122130112
Complete genome sequence of the fish pathogen Flavobacterium branchiophilum.Members of the genus Flavobacterium occur in a variety of ecological niches and represent an interesting diversity of lifestyles. Flavobacterium branchiophilum is the main causative agent of bacterial gill disease, a severe condition affecting various cultured freshwater fish species worldwide, in particular salmonids in Canada and Japan. We report here the complete genome sequence of strain FL-15 isolated from a diseased sheatfish (Silurus glanis) in Hungary. The analysis of the F. branchiophil ...201121926215
time trends in fish populations in metropolitan france: insights from national monitoring data.using the electrofishing database of the french national agency for water and aquatic environment (onema), the time trends of 48 freshwater fish taxa at 590 sites monitored for at least 8 years from 1990 to 2009 were investigated. the results demonstrated that species richness increased steadily from the beginning of the monitoring period. this is congruent with the finding that the number of species displaying a significant increase in spatial distribution or abundance was greater than those sh ...201122136235
patterns of mercury and methylmercury bioaccumulation in fish species downstream of a long-term mercury-contaminated site in the lower ebro river (ne spain).since the 19th century, large amounts of industrial waste were dumped in a reservoir adjacent to a chlor-alkali plant in the lower ebro river (ne spain). previous toxicological analysis of carp populations inhabiting the surveyed area have shown that the highest biological impact attributable to mercury pollution occurred downstream of the discharge site. however, mercury speciation in fish from this polluted area has not been addressed yet. thus, in the present study, piscivorous european catfi ...201121663932
intestinal immune response of silurus glanis and barbus barbus naturally infected with pomphorhynchus laevis (acanthocephala).immunopathological and ultrastructural studies were conducted on the intestine of barbel barbus barbus and sheatfish silurus glanis that were naturally infected with the acanthocephalan pomphorhynchus laevis. enteric helminths often cause inflammation of the digestive tract, inducing the recruitment of different types of immune cells at the site of infection. the results of our study clearly demonstrated that mast cells (mc) were the dominant immune cells which occur at the site of inflammation ...201121226724
expression analysis of immune response genes in fish epithelial cells following ranavirus infection.ranaviruses (family iridoviridae) are a growing threat to fish and amphibian populations worldwide. the immune response to ranavirus infection has been studied in amphibians, but little is known about the responses elicited in piscine hosts. in this study, the immune response and apoptosis induced by ranaviruses were investigated in fish epithelial cells. epithelioma papulosum cyprini (epc) cells were infected with four different viral isolates: epizootic haematopoietic necrosis virus (ehnv), fr ...201222452879
mercury and selenium concentrations in muscle tissue of different species of predatory freshwater fish and correlation between these elements.concentrations of total mercury and selenium were determined in 49 and 42 muscle tissue samples, respectively, of six species of predatory freshwater fish, dace (leuciscus leuciscus), pike perch (sander lucioperca), pike (esox lucius), european catfish (silurus glanis), rainbow trout (oncorhynchus mykiss) and asp (aspius aspius). muscle selenium concentration did not correlate with the corresponding total mercury concentration (r² < 0.198) in all examined predatory fish species. there was an inv ...201224779785
complete genome sequence of the european sheatfish virus.viral diseases are an increasing threat to the thriving aquaculture industry worldwide. an emerging group of fish pathogens is formed by several ranaviruses, which have been isolated at different locations from freshwater and seawater fish species since 1985. we report the complete genome sequence of european sheatfish ranavirus (esv), the first ranavirus isolated in europe, which causes high mortality rates in infected sheatfish (silurus glanis) and in other species. analysis of the genome sequ ...201222570241
concentrations of microcystins in tissues of several fish species from freshwater reservoirs and ponds.the aim of this study is to summarise the determination of concentrations of microcystins (mcs) in muscle and liver of freshwater fish species caught in stagnant waters of the czech republic. within the years 2007-2009, 351 muscle samples and 291 liver samples of 16 freshwater fish species derived from four fishponds, and four water reservoirs were analysed. mcs were detected in 53 liver samples. the highest concentrations of microcystins were determined in liver samples of carnivorous fish spec ...201323756815
the accumulation and distribution of metals in water, sediment, aquatic macrophytes and fishes of the gruža reservoir, serbia.the concentrations of iron, lead, cadmium, copper, manganese, mercury and arsenic were measured in water, sediment, five macrophytes (typha angustifolia, iris pseudacorus, polygonum amphybium, myriophyllum spicatum and lemna gibba) and five fish species (sander lucioperca, abramis brama, carassius gibelio, silurus glanis and arystichtys nobilis) in the gruža reservoir, used for water supply and recreational fishing. the concentrations of all examined elements were higher in sediment than in wate ...201323412697
content of polychlorinated dibenzo-p-dioxins, dibenzofurans and dioxin-like polychlorinated biphenyls in fish from latvian lakes.seventeen polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (pcdd/pcdfs) of the highest priority as well as twelve dioxin-like polychlorinated biphenyls (dl-pcbs) were analyzed in the muscle tissues of the following freshwater fish species sampled from eleven latvian freshwater lakes: perch (perca flavescens), carp (cyprinus carpio), eel (anguilla rostrata), bream (abramis brama), chub (leuciscus cephalus), pike (esox lucius), sheatfish (silurus glanis) and roach (rutilus). to ...201323336927
distribution and relationships of as, cd, pb and hg in freshwater fish from five french fishing areas.the concentrations of arsenic, cadmium, mercury and lead in 149 muscle samples of eight freshwater fish species (european eel, bream, common carp, european catfish, roach, perch, pike and pikeperch) from five different french fishing areas from contaminated and control sites were measured by inductively coupled plasma mass spectrometry after microwave digestion under pressure. no significant correlation was found between the condition factor (cf), based on the length-mass relationship, and as, c ...201323177713
heavy metal and trace element bioaccumulation in target tissues of four edible fish species from the danube river (serbia).pikeperch (sander lucioperca), european catfish (silurus glanis), burbot (lota lota), and common carp (cyprinus carpio) were collected from the danube river (belgrade section, serbia), and samples of liver, muscle, and gills were analyzed for al, as, b, ba, cd, co, cr, cu, fe, hg, li, mn, mo, ni, pb, se, sr, and zn using inductively coupled plasma optical emission spectrometry (icp-oes) to highlight the importance of species and tissue selection in monitoring research, contaminant studies, and h ...201324054751
distribution and accumulation of elements (as, cu, fe, hg, mn, and zn) in tissues of fish species from different trophic levels in the danube river at the confluence with the sava river (serbia).pikeperch (sander lucioperca), european catfish (silurus glanis), common carp (cyprinus carpio), and gobies (neogobius gymnotrachelus, neogobius melanostomus) were collected from the danube river (belgrade section), and samples of liver, muscle, or whole-body composites (in the case of gobies) were analyzed for as, cu, fe, hg, mn, and zn with inductively coupled plasma optical spectrometry to find out if there was a correlation between accumulation of these elements in predatory and prey species ...201323389858
effects of temperature on auditory sensitivity in eurythermal fishes: common carp cyprinus carpio (family cyprinidae) versus wels catfish silurus glanis (family siluridae).in ectothermal animals such as fish, -temperature affects physiological and metabolic processes. this includes sensory organs such as the auditory system. the reported effects of temperature on hearing in eurythermal otophysines are contradictory. we therefore investigated the effect on the auditory system in species representing two different orders.201425255456
gill reaction to pollutants from the tamiš river in three freshwater fish species, esox lucius l. 1758, sander lucioperca (l. 1758) and silurus glanis l. 1758: a comparative study.the study evaluated the effects of waterborne pollutants from the tamiš river on gill histology and possible differences in gill reaction patterns between three freshwater fish species, pike esox lucius l. 1758, pike-perch sander lucioperca (l. 1758) and wels catfish silurus glanis l. 1758 from the tamiš river. gills from analysed fish species showed moderate to intense histopathological alterations. the most frequent progressive alteration was hyperplasia of epithelium, whereas the most frequen ...201524809962
distribution and age-related bioaccumulation of lead (pb), mercury (hg), cadmium (cd), and arsenic (as) in tissues of common carp (cyprinus carpio) and european catfish (sylurus glanis) from the buško blato reservoir (bosnia and herzegovina).the purpose of this study was to quantify the bioaccumulation of pb, hg, cd, and as in tissues of carp (cyprinus carpio) and catfish (silurus glanis) from buško blato in bosnia and herzegovina. arsenic concentrations were below the maximal admissible concentration (mac) for croatia and other countries. mercury concentrations were below 1 mg kg(-1), but in most muscle samples of both species and all catfish liver samples, the values were higher than 0.5 mg kg(-1) (higher than the mac for many cou ...201525966047
biomass and abundance biases in european standard gillnet sampling.the european standard en 14757 recommends gillnet mesh sizes that range from 5 to 55mm (knot-to-knot) for the standard monitoring of fish assemblages and suggests adding gillnets with larger mesh sizes if necessary. our research showed that the recommended range of mesh sizes did not provide a representative picture of fish sizes for larger species that commonly occur in continental europe. we developed a novel, large mesh gillnet which consists of mesh sizes 70, 90, 110 and 135mm (knot to knot, ...201525793776
outbreak of ranavirus infection in sheatfish, silurus glanis (l.), in poland.ranavirus was detected in adult sheatfish, with clinical signs, on a polish fish farm in february. farm isolates induced a strong cytopathic effect in vitro and were identified by electron microscopy and pcr amplification of the ranavirus specific gene fragment. restriction analysis with the acc i enzyme showed that isolated ranaviruses were different from the epizootic heamatopoietic necrosis virus (ehnv). we sequenced a fragment of the major capsid protein (mcp) gene and found that isolates we ...201526618594
occurrence and intensity of parasites in wels catfish, silurus glanis l. 1758 from amirkelayeh wetland, southwest of the caspian sea.amirkelayeh international wetland with a surface area of 1,230 hectares and fifteen fish species is an important water resource in the southern part of the caspian sea. there is few reports on fish parasites of this area. in the present study, a total of 80 wels catfish, silurus glanis, were collected from april through july 2011. after recording biometric characteristics, common necropsy and parasitology methods were used. in the present study, a total of 441 individuals of seven parasite speci ...201627605796
whole genome sequencing and phylogenetic characterization of brown bullhead (ameiurus nebulosus) origin ranavirus strains from independent disease outbreaks.ranaviruses are emerging pathogens associated with high mortality diseases in fish, amphibians and reptiles. here we describe the whole genome sequence of two ranavirus isolates from brown bullhead (ameiurus nebulosus) specimens collected in 2012 at two different locations in hungary during independent mass mortality events. the two hungarian isolates were highly similar to each other at the genome sequence level (99.9% nucleotide identity) and to a european sheatfish (silurus glanis) origin ran ...201627717748
spatial monitoring of heavy metals in the inland waters of serbia: a multispecies approach based on commercial fish.the study monitored the contamination of fish muscle tissue by elements al, as, cd, co, cr, cu, fe, hg, mn, ni, pb, and zn at 17 sampling sites, in order to assess the pollution status of the main rivers in serbia. of the six commercially important fish species included in the study (pikeperch sander lucioperca, catfish silurus glanis, bream abramis brama, barbel barbus barbus, chub squalius cephalus, nase chondrostoma nasus), the bioconcentration factor (bcf) indicated that benthivore bream and ...201626857004
a fish model for the study of the relationship between neuroendocrine and immune cells in the intestinal epithelium: silurus glanis infected with a tapeworm.immunohistochemical, immunofluorescence and ultrastructural studies were conducted on a sub-population of 20 wels catfish silurus glanis from a tributary of the river po (northern italy). fish were examined for the presence of ecto- and endo-parasites; in the intestine of 5 fish, 11 specimens of cestode glanitaenia osculata were noted and was the only helminth species encountered. the architecture of intestine and its cellular features were nearly identical in either the uninfected s. glanis or ...201728330806
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