Publications

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effects of glucose on the uptake and metabolism of glycine in pakchoi (brassica chinensis l.) exposed to various nitrogen sources.plants can absorb amino acids as a nitrogen (n) source, and glucose is an important part of root rhizodeposition and the soil sugar pool, which participates in the regulation of plant growth and uptake. in pakchoi, the effect of glucose concentration on the glycine n uptake from a nutrient mixture composed of glycine, ammonium, and nitrate, or from a single n solution of glycine alone was studied using specific substrate (15)n-labeling and (15)n-gas chromatography mass spectrometry.028253854
slow-release nitrogen fertilizers can improve yield and reduce cd concentration in pakchoi (brassica chinensis l.) grown in cd-contaminated soil.cadmium (cd) pollution in vegetable crops has become a serious problem in recent years. owing to the limited availability of arable land resources, large areas of cd-contaminated lands are inevitably being used for the production of vegetables, posing great risks to human health via the food chain. however, strategies to improve yield and reduce cd concentration in crops grown in contaminated soils are being developed. in the present study, using pot experiments, we investigated the effects of t ...027677996
extracts of tsai tai (brassica chinensis): enhanced antioxidant activity and anti-aging effects both in vitro and in caenorhabditis elegans.tsai tai is one of the most widely consumed brassica vegetables in asian countries because of its good taste and its nutritional benefits. this study evaluated the antioxidant capacity and possible associated health benefits of 3 tsai tai (brassica chinensis) varieties, namely, hon tsai tai, pak choi and choi sum. the dpph radical scavenging ability and reducing power assays were performed to evaluate the in vitro activities of the extracts. caenorhabditis elegans was used as an in vivo model fo ...026726147
[residue and degradation of roxarsone in the system of soil-vegetable].the field experiment was developed for simulating the residues, transformation and degradation in soil-vegetable system of roxarsone contained in organic fertilizer. under the treatment, the yield of brassica chinensis decreased in low roxarsone concentration with a decline by 15% to 32% compared with the control group; there had an accumulating role of vegetables to arsenic, and the root was the main part; total content of arsenic in the soil was positively correlated with the dose of the appli ...026592042
dissipation and distribution of chlorpyrifos in selected vegetables through foliage and root uptake.dissipation, distribution and uptake pathways of chlorpyrifos were investigated in pakchoi (brassica chinensis l.) and lettuce (lactuca sativa) with foliage treatments under a greenhouse trial and root treatments under a hydroponic experiment. the dissipation trends were similar for chlorpyrifos in pakchoi and lettuce with different treatments. more than 94% of chlorpyrifos was degraded in the samples for both of the vegetables 21 days after the foliage treatments. for the root treatment, the di ...026363321
composite of pah-degrading endophytic bacteria reduces contamination and health risks caused by pahs in vegetables.vegetables accumulate polycyclic aromatic hydrocarbons (pahs) at high concentrations when grown in contaminated sites. inoculation with pah-degrading endophytic bacteria (ebpah) has been recognized as one of the most promising ways to remove pahs from plant bodies; however, the performance of single endophytic bacteria is generally limited. this investigation used a composite of eight ebpah to reduce the contamination and health risk posed by 16 epa priority pahs in vegetables including chinese ...028448936
improved discrimination for brassica vegetables treated with agricultural fertilizers using a combined chemometric approach.multielement and stable isotope (δ(13)c, δ(15)n, δ(2)h, δ(18)o, (207)pb/(206)pb, and (208)pb/(206)pb) analyses were combined to provide a new chemometric approach to improve the discrimination between organic and conventional brassica vegetable production. different combinations of organic and conventional fertilizer treatments were used to demonstrate this authentication approach using brassica chinensis planted in experimental test pots. stable isotope analyses (δ(15)n and δ(13)c) of b. chinen ...027355562
[comparative analysis of chloroplasts isolated from healthy and turnip yellow mosaic virus-infected brassica chinensis plants]. 19725034445
the effects of gelatin on aggregation of protoplasts from higher plants.aggregation of protoplasts of allium fistulosum l., brassica chinensis l. and daucus carota l. was induced in great frequency with gelatin and early products of gelatin degradation. the optimal gelatin concentration was 2-5%. the aggregated protoplasts fused into groups and cell walls were formed around them in culture.197324458819
cysteine synthase from rape leaves.cysteine synthase [o-acetyl-l-serine acetate-lyase (adding hydrogen-sulfide) ec 4.2.99.8] has been highly purified from the extract of rape, brassica chinensis var. komatsuna. the purified preparation appeared to be homogeneous on sephadex g-100 gel filtration and dodecylsulfate-polyacrylamide gel electrophoresis, showing a molecular weight of about 62,000. the latter method also suggested that this enzyme was composed of two identical subunits. the enzyme contained 2 moles of pyridoxal phosphat ...19751158855
intracellular location of carbonate dehydratase (carbonic anhydrase) in leaf tissue.two proteins which have carbonate dehydratase (carbonic anhydrase, ec 4.2.1.1) activity were shown to be in the chloroplasts and in the cytosol of leaves of brassica chinensis, spinacia oleracea, and in variegated leaves of tradescantia albiflora and hedera canariensis. the chloroplastic enzyme is smaller than the one in the cytosol, as it runs farther on gradient polyacrylamide gels. it was separated from the other by isolation of chloroplasts of brassica and spinacia on sucrose density gradien ...197916660683
simultaneous measurements of steady state chlorophyll a fluorescence and co(2) assimilation in leaves: the relationship between fluorescence and photosynthesis in c(3) and c(4) plants.rates of co(2) assimilation and steady state chlorophyll a fluorescence were measured simultaneously at different intercellular partial pressures of co(2) in attached cotton (gossypium hirsutum l. cv deltapine 16) leaves at 25 degrees c. electron transport activity for co(2) assimilation plus photorespiration was calculated for these experiments. under light saturating (1750 microeinsteins per square meter per second) and light limiting (700 microeinsteins per square meter per second) conditions ...198616664735
gradients of intercellular co(2) levels across the leaf mesophyll.most current photosynthesis models, and interpretations of many wholeleaf co(2) gas exchange measurements, are based on the often unstated assumption that the partial pressure of co(2) is nearly uniform throughout the airspaces of the leaf mesophyll. here we present measurements of co(2) gradients across amphistomatous leaves allowed to assimilate co(2) through only one surface, thus simulating hypostomatous leaves. we studied five species: eucalyptus pauciflora sieb. ex spreng., brassica chinen ...198816666027
decontamination of landfill leachate by soil infiltration.a soil column study was conducted to evaluate the efficiency of soil to attenuate pollutants in landfill leachate. it was found that more than 60% of the initial alkalinity, cod, and total nitrogen was removed after the leachate had percolated through the soil column. lower efficiencies were observed for the removal of dissolved cations, ammonia nitrogen, and nitrate nitrogen. when previously percolated leachate was recirculated through the soil column, significant removal (93.6%) of ammonia nit ...19892604902
toxicity tests of soil contaminated by recycling of scrap plastics.the present investigation studied the toxicity of soil contaminated by untreated discharge from a factory that recycles used plastics. the nearby agricultural areas and freshwater fish ponds were polluted with high concentrations of cu, ni, and mn. water extracts from the contaminated soil retarded root growth of brassica chinensis (chinese white cabbage) and cynodon dactylon (bermuda grass) where their seeds were obtained commercially. the contaminated populations of c. dactylon, panicum repen ...19902331327
decontamination of landfill leachate by soils with different textures.soils with different textures (sandy, loamy, and clay soils) were used as filters to attenuate leachate from the gin drinkers' bay landfill. they were used to pack columns of different depths: 0.2, 0.6, and 1.0 m. eight millimeters of leachate was drained into the soil columns each day for 56 days. the percolated leachates were collected weekly and their properties analyzed. it was revealed that the properties became rather stable at day 28 and therefore only the data from day 28 are presented. ...19902096848
brassinosteroid stimulation of hypocotyl elongation and wall relaxation in pakchoi (brassica chinensis cv lei-choi).hypocotyl elongation of pakchoi (brassica chinensis cv lei-choi) was stimulated by applying 300 ng of brassinosteroid (2[alpha],3[alpha],22[beta],23[beta]-tetrahydroxy-24[beta]-methyl-b-homo-7- oxa-5[alpha]-cho-le stan-6-one, br) in 1 [mu]l of 50% ethanol to the apex of hypocotyls. br had its greatest effect on elongation of the apical 3-mm region below the cotyledonary node (75% stimulation) between 6 and 18 h after treatment. stress/strain (instron) analysis of this 3-mm region revealed that p ...199312231748
studies of the biosynthesis of tentoxin by alternaria alternata.biosynthesis of the phytotoxin, tentoxin, its regulation and the enzymic synthesis steps were studied in vivo and in vitro. the physiology of biosynthesis of tentoxin in vivo was investigated by using sections of mycelial mats incubated in buffer. differentiated mycelia could be studied under defined conditions. the de novo synthesis of tentoxin was measured by incorporation of [u-14c]leucine into tentoxin. the investigation system was stable for 10 h. biosynthesis and the growth of biomass star ...19947881545
responses of some common cruciferae to root-knot nematodes.ten cultivated plants of the family cruciferae were evaluated for susceptibility to meloidogyne arenaria race 1, m. incognita races 1 and 3, and m. javanica in a series of four separate greenhouse tests. after 62-64 days, or 1,032-1,072 degree days (10 c base), several of the crops evaluated showed moderate to severe levels of galling (> 3.0 on 0-5 scale) and moderate numbers of egg masses (>2.0 on 0-5 scale) in response to each of the nematode species and races. among the plants tested, collard ...199519277321
[chemoprevention of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine-induced carcinogen-dna adducts by chinese cabbage in rats].to examine the preventive effect of chinese cabbage (brassica chinensis) against colon carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip)-induced phip-dna adduct formation in rats and its possible mechanism.199810920929
assessment of the transfer of 137cs in three types of vegetables consumed in hong kong.a dynamic food chain model has been built for the modeling of the transfer of 137cs in three types of vegetables consumed in hong kong, namely, white flowering cabbage (brassica chinensis), head lettuce (lactuca sativa) and celery (apium graveolens). some parameters have been estimated from the experimental data obtained in this work. the experimental data include the transfer factors of 137cs from soil to the different vegetable species which are determined through high resolution gamma spectro ...19989745700
foliar modifications induced by inhibition of polar transport of auxin.the effects of auxin polar transport inhibitors, 9-hydroxy-fluorene-9-carboxylic acid (hfca); 2, 3, 5-triiodobenzoic acid (tiba) and trans-cinnamic acid (ca) on leaf pattern formation were investigated with shoots formed from cultured leaf explants of tobacco and cultured pedicel explants of orychophragmus violaceus, and the seedlings of tobacco and brassica chinensis. although the effective concentration varies with the inhibitors used, all of the inhibitors induced the formation of trumpet-sha ...199910321686
[preliminary studies on tissue culture and agrobacterium-medicated transformation of brassica campestris ssp. chinensis].the hypocotyls and cotyledons of the asepetic seedling of brassica campestris ssp. chinensis l cv. pudongaijiecai) were used as explants for tissue culture. adventitious buds were differentiated on modified ms medium supplemented with tdz 1-2 mg/l, naa 0.2-1 mg/l and agno3 7.5 mg/l. the percentage of explants which formed buds of cotyledons was about 56%, and that of hypocotyls was about 37%. when the regenerated explants were transferred onto ms medium with 2 i.p. 5 mg/l and naa 0.1 mg/l for tw ...199912548800
chemoprevention of 2-amino-1-methyl-6-phenyli-midazo 4,5-b pyridine-induced carcinogen-dna adducts by chinese cabbage in rats.aim:the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo 4,5-b pyridine (phip) induces colon and mammary gland tumors in rats and has been implicated in the etiology of human colorectal cancer.this study was conducted to examine the potentially preventive effect of chinese cabbage (brassica chinensis),a brassica vegetable most commonly consumed in china, against this carcinogen-induced dna adduct formation in rats and its possible mechanisms.methods:sprague-dawley rats were maintained for ...199911819413
identification and characterization of the inward k+ channel in the plasma membrane of brassica pollen protoplasts.patch clamp techniques have been used to identify and characterize the whole-cell currents carried by inward k+ channels in isolated matured pollen protoplasts of brassica chinensis var. chinensis. the whole-cell inward currents in the isolated pollen protoplasts were activated at hyperpolarized membrane potentials more negative than -100 mv. the magnitudes of the whole-cell inward currents were strongly dependent on the external k+ concentration, and were highly selective for k+ over other mono ...199910555306
[study on activity of inner inhibitory substances of acanthopanax senticosus (rupr. et maxim) harms fruits and seeds].by studying on the inner inhibitory substances of acanthopanax senticosus fruits and seeds, the reason for dormancy of the seeds was investigated.200112776367
[reclamation of the plant regeneration efficiency of brassica campestris subsp. chinensis var.parachinensis].this investigation has developed an efficient and fast method for plant regeneration from petiole of cotyledon explants of brassica campestris l. subsp. chinensis makino var. parachinensis tsen et lee. a medium was designed for b. campestris subsp. chinensis var. parachinensis to obtain the high frequency of shoot regeneration, which contained bap 2 mg/l, naa 0.75-1.0 mgl and 7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium. 60 ml/l coconut milk were added to all of me ...200112549111
toxicity evaluation of sewage sludges in hong kong.anaerobically digested sewage sludges collected from four wastewater treatment plants located in sha tin, tai po, yuen long, and shek wu hui in hong kong were subjected to chemical characterization and toxicity testing to provide preliminary assessment of their suitability for land application. all sewage sludges were slightly alkaline with ph range of 8.3-8.7. electrical conductivity (ec; 0.69 ds m(-1)) and soluble nh4-n content (996 mg kg(-1)) of sewage sludge from yuen long were lower than th ...200111757851
sequence analysis shows that ribgrass mosaic virus shanghai isolate (rmv-sh) is closely related to youcai mosaic virus.the complete nucleotide sequence of an isolate of ribgrass mosaic virus (rmv-sh) from brassica chinensis (qingcai) in shanghai, china was determined. the genome consisted of 6301 nucleotides and its genomic organization was similar to those of other crucifer-tobamoviruses. comparisons of the nucleotide and predicted amino acid sequences and phylogenetic analyses showed that rmv-sh had very high homology (> 95% identical nucleotides and 97.7-99.6% identical amino acids) to a sequence of youcai mo ...200111504428
effect of applying hong kong biosolids and lime on nutrient availability and plant growth in an acidic loamy soil.a greenhouse experiment was conducted to evaluate the growth of brassica chinensis l. in an acidic loamy soil amended with municipal biosolids with or without lime treatment (750 mg kg(-1)). the soil was amended with one of two municipal biosolids from tai po (tp) or yuen long (yl) wastewater treatment plants at application rates of 0, 5, 10, 25 and 50% (v/v). initial nh(+)4-n and po(3-)4-p concentrations in the amended soil increased with an increase in municipal biosolids loading rates and tho ...200111873884
assessing copper thresholds for phytotoxicity and potential dietary toxicity in selected vegetable crops.copper pollution in soils is widespread, and its accumulation in crop products could pose a risk on human health. in this paper, bioavailability of added copper (cu) and critical cu concentrations in a vegetable garden soil was evaluated for chinese cabbage (brassica chinensis l.), pakchoi (brassica chinensis l.), and celery (apiumg graveolens l. var. dulce dc) based on human dietary toxicity. the availability of added cu in the soil decreased with incubation time, and had minimal change after 1 ...200212403270
[response of some protective enzymes in brassica chinensis seedlings to pb2+, cd2+ and cr6+ stresses].seedlings of brassica chinensis were cultured in 1/2 hoagland solution for seven days before they were treated with pb2+, cd2+ and cr6+. the activities of their superoxide dismutase (sod), peroxidase (pod) and catalase (cat) responded to the increasing concentrations of the metals, and the responsive characters varied with different metals and different durations of metal treatments. cat activity decreased with the increase of metal concentrations, and the order of its sensitivity to the three m ...200212222065
activation of antigen-presenting cells by immunostimulatory plant dna: a natural resource for potential adjuvant.genomic dna sequences (bacteria, insect, nematodes and molluscs) or synthetic oligodeoxynucleotides (odn) containing unmethylated cpg motifs (cpg-dna/odn) are regarded as promising candidates for new medical adjuvants for their ability to stimulate the mammalian immune system and enhance immune responses to specific antigens. here, we first report the immunostimulatory activity of total genomic dna from two plants, brassica chinensis l. and zea may, the cpg methylation status of which is incompl ...200212034103
evaluation of major constraints to revegetation of lead/zinc mine tailings using bioassay techniques.the residues from the extraction of lead/zinc (pb/zn) ores of most pb/zn mines are permanently stored in tailings ponds, which require revegetation to reduce their environmental impact. this can only be done if the main constraints on plant establishment are evaluated. this can readily be done by field and greenhouse studies. to test this, the properties of different tailings from lechang pb/zn mine located at the north of guangdong province in southern china have been studied. physical and chem ...200212137044
[agricultural application of sludge dredged from landscape water bodies].the feasibility and ecological effects of agricultural application of sludge dredged from the grand canal (hangzhou section) were studied. applied too much dredged sludge to red soil and paddy soil affected the germination of alpine fescue [fescuta ovina var. brachyphylla (schult.) piper] and colver (trifolium repens. l) seeds, while there wasn't significantly affects in pot experiment. while the application rate was lower than 270 t.hm-2, the growth of pakchoi (brassica chinensis l.) increased ...200212132166
phytotoxicity of dredged sediment from urban canal as land application.phytotoxicity of dredged sediment from hangzhou section of the grand canal as land application was evaluated by pakchoi (brassica chinensis l.) germination tests and pot experiments. germination rates of pakchoi in the dredged sediment and in sediment-applied soils were both significantly higher than that in the soil controls, while the germination rate between the sediment-applied soils was no significant difference. in pot experiments, plant height and biomass were increased by the dredged sed ...200211916038
outward k+ channels in brassica chinensis pollen protoplasts are regulated by external and internal ph.patch-clamp whole-cell and single-channel recording techniques were used to investigate the regulation of outward k(+) channels by external and internal protons in brassica chinensispollen protoplasts. outward k(+) currents and conductance were insensitive to external ph (ph(o)) except at ph 4.5. maximal conductance (g(max)) for the outward k(+) currents was inhibited at acidic external ph. half-activation voltage ( e(1/2)) for the outward k(+) currents shifted to more positive voltages along wi ...200312664278
[induced activity of nitrate reductase by nitrate and cloning of nitrate reductase gene].excessive nitrate accumulated in plants affects vegetable quality severely and excessive nitrate ingestion would do harm to human health. assimilatory nadh: nitrate reductase (nr, ec 1.6.6.1), a complex mo-pterin-, cytochrome b(557)- and fad-containing protein, catalyzes the regulated and rate-limiting step in the utilization of inorganic nitrogen by higher plants. enhancing the activity of nr is conducive to reduce the concentration of nitrate in plants. the experiments were conducted to invest ...200315969098
copper and zn uptake by radish and pakchoi as affected by application of livestock and poultry manures.environmental safety of agricultural utilization of livestock and poultry manures from intensive farming is attracting great attention because the manures often contain high concentrations of heavy metals and organic pollutants. pot experiments, in which a pig manure (pm), a chicken manure (cm) and a commercial organic manure (om) with different concentrations of cu and zn to simulate soil metal accumulation by manure application for different times were utilized in a garden soil at a rate of 2% ...200415722088
[accumulation of coat protein of turnip mosaic virus in host chloroplasts and its effect on ps ii activity].intact chloroplasts were isolated from chinese cabbage (brassica chinensis l. cv. suzhou) and mustard (b. juncea l. cv. wenzhou) leaves inoculated with turnip mosaic virus (tumv). the proteins attached to the surface of chloroplasts were digested, then the total proteins of chloroplasts were extracted. by analysis of the sds-page pattern, one extra protein band with the same mobility and molecular weight as tumv-coat protein was found in the chloroplasts of infected leaves. results of western bl ...200415583406
a napin-like polypeptide from dwarf chinese white cabbage seeds with translation-inhibitory, trypsin-inhibitory, and antibacterial activities.napins are 1:1 disulfide-linked complexes of a smaller (ca. 4kda) subunit and a larger (ca. 10kda) subunit. the intent of the present study was to ascertain the production of napin by the seeds of a brassica species that has not been examined previously, and also to explore new biological activities of the napin. a heterodimeric 11-kda napin-like polypeptide has been isolated from chinese white cabbage (brassica chinensis cv dwarf) seeds with a protocol comprising ion exchange chromatography on ...200415062997
selecting iodine-enriched vegetables and the residual effect of iodate application to soil.a greenhouse pot experiment was conducted to select vegetables for iodine uptake. the residual effect of iodate fertilization on the growth of and iodine uptake by spinach plants were also investigated. six vegetables, including leafy vegetables (pakchoi [brassica chinensis l.], spinach [spinacia oleracea l.]), tuber vegetables (onion [allium cepa l.]), shoot vegetables (water spinach [ipomoea aquatica forsk.], celery [apium graveolens l.]), and root vegetables (carrot [daucus carota var. sativa ...200415564656
[isolation and characterization of bcmf3, a gene expressed only in maintainer line in chinese cabbage-pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee)].expression profiling was performed using cdna-aflp technology on floral buds of a genic male sterile line (a line) and a maintainer line (b line) in chinese cabbage pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee cv. aijiaohuang). a differentially expressed cdna fragment, ba18-t16, was obtained via selective amplification with a18/t16 primer pair. ba18-t16 was specifically associated with the maintainer line, and was expressed only in floral buds revealed by half ...200415651684
plant wounding affects the oviposition of hellula undalis(f.). the impact of leaf chemistry.crucifer specialists, such as the tropical pest hellula undalis (f.), developed a close association to their hosts and depend on characteristic secondary plant compounds, the glucosinolates (gs), in many cases. following tissue damage, gs undergo hydrolysis catalyzed by the enzyme myrosinase to produce a complex array of products which include isothiocyantes, cyanides, and thiocyanates depending on reaction condition and the chemical nature of the side chain. we found that mechanical plant damag ...200415759429
[the cytochemical observation of anthers of chinese cabbage's male-sterile].a chinese cabbage (brassica campestris l. ssp. chinensis makino) produces 1/4 male sterile and 3/4 fertility in offspring. the sterile plant can be identified from the color of corolla that is some white when it grows out. the fertile and sterile anthers were researched using cytological and cytochemical methods. thick sections of both anthers of different developmental stages were stained with toluidine blue for general cytological observation and stained with the periodic-acid-schiff's (pas) t ...200415511072
genotypic differences in effects of cadmium exposure on plant growth and contents of cadmium and elements in 14 cultivars of bai cai.fourteen cultivars of bai cai (brassica campestris l. ssp. chinensis var. communis) were grown in the nutrient solutions containing 0-0.5 microg ml(-1) of cadmium (cd) to investigate genotypic differences in the effects of cd exposure on the plant growth and uptake and distribution of cd in bai cai plants. the cd exposure significantly reduced the dry and fresh weights of roots and shoots, the dry weight ratio of shoot/root (s/r), total biomass, and chlorophyll content (spad value). cd concentra ...200415473646
[establishment of an efficient regeneration system for chinese cabbage cultured in vitro and histological observation of shoot development].an efficient plant regeneration system from explants of petiole with cotyledon was developed for chinese cabbage (brassica campestris ssp. chinensis makino) in our study. high frequency of shoot regeneration was obtained in the medium containing bap 2 mg/l, naa 0.45 mg/l and 5-7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium, with 84.3 percent being the highest frequency of shoot regeneration in seven chinese cabbage cultivars and one parentage, and the new formed shoo ...200415259989
[ecological toxicity of aluminum-based coagulant on representative corps in neutral environment].by the terrestrial ecotoxicological methods of seed germination and root elongation, and with aluminum-based coagulant alcl3 which was widely used in wastewater- and water treatment as an example, this work studied the toxicological effects of aluminum-based coagulants on representative crops, including radish raphanus sativus, cabbage brassica chinensis and wheat triticum aestivum, over a range of exposure concentrations under the neutral condition (ph= 7.0), with contrast to an acid condition ...200516471362
toxic effects of al-based coagulants on brassica chinensis and raphanus sativus growing in acid and neutral conditions.the ecotoxicological effects of aluminum (al)-based coagulants are of concern because of their wide-ranging applications in wastewater treatment and water purification. as important al-based coagulants, alcl(3) and pac (polyaluminum-chloride) were selected as examples to examine the toxic effects on representative vegetables including the cabbage brassica chinensis and the radish raphanus sativus over a range of exposure concentrations in neutral (ph 7.00) and acidic (ph 4.00) conditions, using ...200515793823
[the ultrastructural observation of anthers of chinese cabbage's mail-sterility].the fertile and sterile anthers of a chinese cabbage (brassica campestris l. ssp. chinensis makino) were observed using electron microscope to find the ultrastructural feature of sterile anthers. the earliest abnormal phenomenon in sterile anther was nucleolus of sporogenous cells locating in the edge of nucleus. during microspore mother cell development, callus wall surrounding the cell displayed uneven in the thick ness and was discontinuous,and the some cytoplasm leaked out of the cell from s ...200516416967
[calcium distribution in fertile and sterile anthers of a genic male sterile chinese cabbage].potassium antimonite was used to locate calcium in the fertile and sterile anthers of a genic male sterile chinese cabbage (brassica campestris l. ssp. chinensis makino) to probe the relation between ca(2+) and fertility and sterility of anthers of the cabbage. during fertile anther development, calcium granules increase in number in anther wall cells after meiosis, and then appeared also in locule, suggesting a calcium influx into locule from anther wall cells (plate i-4). then the number of ca ...200516361789
role of nitrification inhibitor dmpp (3, 4-dimethylpyrazole phosphate) in no(3-)-n accumulation in greengrocery( brassica campestris l. ssp. chinensis) and vegetable soil.the influence of nitrification inhibitor (ni) 3, 4-dimethylpyrazole phosphate (dmpp) on nitrate accumulation in greengrocery (brassica campestris l. ssp. chinensis) and vegetable soil at surface layer were investigated in field experiments in 2002 and 2003. results showed that ni dmpp took no significant effect on yields of edible parts of greengrocery, but it could significantly decrease no(3-)-n concentration in greengrocery and in vegetable soil at surface layer. in addition, ni dmpp could re ...200515900763
plant growth inhibition by cis-cinnamoyl glucosides and cis-cinnamic acid.spiraea thunbergii sieb. contains 1-o-cis-cinnamoyl-beta-d-glucopyranose (cg) and 6-o-(4'-hydroxy-2'-methylene-butyroyl)-1-o-cis-cinnamoyl-beta-d-glucopyranose (bcg) as major plant growth inhibiting constituents. in the present study, we determined the inhibitory activity of cg and bcg on root elongation of germinated seedlings of lettuce (lactuca sativa), pigweed (amaranthus retroflexus), red clover (trifolium pratense), timothy (phleum pratense), and bok choy (brassica rapa var chinensis) in c ...200515898503
soil to plant transfer of 238u, 226ra and 232th on a uranium mining-impacted soil from southeastern china.both soil and plant samples of nine different plant species grown in soils from southeastern china contaminated with uranium mine tailings were analyzed for the plant uptake and translocation of 238u, 226ra and 232th. substantial differences were observed in the soil-plant transfer factor (tf) among these radionuclides and plant species. lupine (lupinus albus) exhibited the highest uptake of 238u (tf value of 3.7x10(-2)), while chinese mustard (brassica chinensis) had the least (0.5x10(-2)). how ...200515878419
[distribution of rubisco and rca in brassica chinensis chloroplasts and effect of tumv-infection on their cellular localization].the cellular localizations of rubisco and rubisco activase (rca) in chinese cabbage (brassica chinensis l. cv. suzhou) leaves were investigated by immunogold-labeling electron microscopy. the results showed that rubisco and rca were mainly located in chloroplasts of mesophyll, guard cell of stomatal apparatus and parenchyma of vascular bundle. a high density of gold particles were localized preferentially to the chloroplast stroma. in contrast, there were no specific binding of gold particles de ...200515839204
a comparison of concentration ratios for technetium and nutrient uptake by three plant species.technetium (tc) is a non-essential element for which accumulation mechanisms in plants have recently been discussed, but only from the viewpoint of existence of anion transport proteins in plant cells. in this study, using three kinds of plants (cucumis sativus l., raphanus sativus l., and brassica chinensis l.), uptake of tc and re (a chemical analogue of tc) were observed. the results showed that tc and re uptake occurred not only with water mass flow or active nutrient uptake, but also with u ...200515893799
copper and lead levels in two popular leafy vegetables grown around morogoro municipality, tanzania.a study was carried out to determine the levels of two heavy metals, lead (pb) and copper (cu), in two popular leafy vegetables grown around morogoro municipality in tanzania. vegetable samples of pumpkin leaves (cucurbita moschata) and chinese cabbage (brassica chinensis) were collected from three sites and analysed for their concentrations of the two metals using an atomic absorption spectrophotometer. the three sites, namely mazimbu, kihonda and towelo are located within a 10 km perimeter. th ...200617058799
[bcmf4 gene, encoding a leucine-rich repeat protein, plays a role in male fertility in chinese cabbage-pak-choi].the bcmf4 (brassica campestris male fertility 4) gene was previously isolated from the fertile b line of chinese cabbage-pak-choi (brassica campestris ssp. chinensis var. communis, syn. b. rapa ssp. chinensis var. communis). in the present paper, based on the cdna sequence of bcmf4, primers were designed and used to amplify two fragments from the cdna of flower buds of chinese cabbage-pak-choi. two produced fragments were introduced separately into binary vector pbi121 in antisense and sense ori ...200617098713
[molecular cloning and characterization of a novel wrky gene from brassica chinensis].a new wrky gene was cloned from brassica chinensis by rapid amplification of cdna ends (race). the full-length cdna of bcwrky was 1175 bp long and contained a 924 bp open reading frame (orf) encoding a putative w-box-binding protein of 308 amino acids. the predicted bcwrky protein was found to have a potential bipartite nuclear localization sequence (nls-bp) in its n-terminal region followed by a wrky dna-binding domain. bioinformatic analysis revealed that bcwrky resembled other wrky domain-con ...200617086982
effects of endocrine disruptor di-n-butyl phthalate on the growth of bok choy (brassica rapa subsp. chinensis).the effects of the endocrine disrupter, di-n-butyl phthalate (dbp), on the growth of leaf vegetable bok choy (brassica rapa subsp. chinensis, white stem bok choy) were investigated. the results showed that leaves of bok choy became white in color with the occurrence of chlorosis and necrosis upon treating with 30 mg l(-1) dbp for 42 days. transmission electron microscopic images revealed that changes in the chloroplast structures accompanied the chlorosis. in addition, a decrease in biomass and ...200616824579
extractability and bioavailability of zinc over time in three tropical soils incubated with biosolids.phytotoxicity of heavy metal is the primary concern in applying biosolids (sewage sludge) to agricultural land. this study evaluates the changes in chemical speciation of zn in three tropical soils of taiwan measured with sequential extraction over a one-year period. biosolids were applied to the soils at application rates of 10, 50 and 100 mg ha(-1), and correlated diethylene triamine pentaacetic acid (dtpa) and sequential extraction as extract for prediction of zn bioavailability to chinese ca ...200616213570
effect of bone char application on pb bioavailability in a pb-contaminated soil.the effects of bone char (bc) application on the bioavailability of pb in a polluted soil from hunan province, china were examined. the pb-contaminated soil was treated with two types of bone char, one from the uk and the other from china. the bioavailability of pb was determined in terms of the uptake by chinese cabbage (brassica chinensis l.), sequential extraction and x-ray diffraction analysis. the results indicate that the pb concentrations in both shoots and roots decreased with increasing ...200616095783
functional analysis of a novel male fertility cyp86mf gene in chinese cabbage (brassica campestris l. ssp. chinensis makino).in our earlier work, a cytochrome p450 cyp86mf gene was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa l.) by mrna differential display pcr (dd-pcr) and rapid amplification of cdna ends (race). to unravel the biological function of cyp86mf gene, the antisense fragment from the cyp86mf gene was transferred into chinese cabbage pak-choi (b. campestris ssp. chinensis var. communis tsen et lee). out of 22 plants transformed with the antisense ...200616075226
field demonstration of reduction of lead availability in soil and cabbage (brassica chinensis l.) contaminated by mining tailings using phosphorus fertilizers.a field demonstration of reduction of lead availability in a soil and cabbage (brassica chinensis l.) contaminated by mining tailings, located in shaoxing, china was carried out to evaluate the effects of applications of phosphorus fertilizers on pb fractionation and pb phyto-availability in the soil. it was found that the addition of all three p fertilizers including single super phosphate (ssp), phosphate rock (pr), and calcium magnesium phosphate (cmp) significantly decreased the percentage o ...200616365925
electrostatic application of inert silica dust based insecticides onto plant surfaces.one of the most effective naturally occurring insecticide powders is diatomaceous earth (de), which contains above 96 % of silica (silicon dioxide sio2). in recent days, the possibility to use new improved de formulations for plant protection in horticulture has been the focus of research. for aphids and other under-leaf insects only insecticides deposited on leaf undersides are effective. we tested electrostatic application of different silica containing dusts onto the cruciferous crop pak-choi ...200617390789
pre-visual detection of iron and phosphorus deficiency by transformed reflectance spectra.reflectance spectroscopy and strategies for spectral analysis over the visible range from 380 to 780 nm were used to provide diagnostic information on iron (fe) and phosphorus (p) status of brassica chinensis l. var parachinensis (bailey) grown under hydroponics conditions. leaf reflectance (r) spectra were collected and normalized inner reflectance (nr(i)) spectra were calculated. the regression coefficients (b-matrix) and variable importance for projection (vip) in partial least squares regres ...200616890448
uptake of cadmium by different cultivars of brassica pekinens (lour.) rupr. and brassica chinensis l. and their potential for phytoremediation. 200616688560
dynamics of pesticide residues in the autumn chinese cabbage (brassica chinensis l.) grown in open fields.residues of the pesticides chlorpyrifos, dimethoate, cyhalothrin, cypermethrin, fenvalerate, deltamethrin and chlorothalonil in the autumn chinese cabbage (brassica chinensis l.) were studied using gas chromatography. the results indicated that the residues were dependent on the frequency and rate of pesticide application and on the weather conditions immediately following spraying. when the chinese cabbages were treated once at normal rates, the half-lives of the seven pesticides in the vegetab ...200616506147
influence of lead acetate on soil microbial biomass and community structure in two different soils with the growth of chinese cabbage (brassica chinensis).a greenhouse pot experiment was conducted to evaluate the impact of different concentrations of lead acetate on soil microbial biomass and community structure during growth of chinese cabbage (brassica chinensis) in two different soils. the field soils were used for a small pot, short-term 60-day growth chamber study. the soils were amended with different pb concentrations, ranging from 0 to 900mgkg(-1) soil. the experimental design was a 2 soilx2 vegetation/non-vegetationx6 treatments (pb)x3 re ...200716949632
effects of soil properties on heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) in pearl river delta, china.this study was conducted to investigate the effects of soil properties on the heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) at the field scale. the concentrations of cadmium (cd), mercury (hg), and chromium (cr) in topsoil and vegetable samples from nanhai district of foshan city in the pearl river delta (prd) were analyzed. the results showed that 56.5% of the soil samples exceeded the grade ii of the chinese soil environme ...200717365337
bcmf11, a putative pollen-specific non-coding rna from brassica campestris ssp. chinensis.a full-length cdna, bcmf11, has been cloned from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends (race) based on a pollen-specific cdna fragment (dn237921). the bcmf11 cdna has a total length of 828bp with poly (a) tail. analysis of the sequence demonstrated that bcmf11 is a novel non-coding rna which has no prominent open reading frame (orf) or coding capacity. no significant similarities were observed between bcmf11 and previously publi ...200717207554
use of ammoniacal nitrogen tolerant microalgae in landfill leachate treatment.two microalgae, chlorella pyrenoidosa and chlamydomonas snowiae, were isolated from a high ammonia leachate pond in li keng landfill, guangzhou, china. their growth and nutrient removal rates were determined in a serial dilution of landfill leachate under laboratory conditions, and their growth rates were compared with that of a c. pyrenoidosa strain isolated from a clean river. the results indicated the growth of all three algae was inhibited by high leachate concentrations, and the inhibition ...200717382529
characterization of a novel gene, bcmf7, that is expressed preferentially in pollen of brassica campestris l. ssp. chinensis makino.pollen formation is important for plant sexual reproduction. to identify the genes that are involved in pollen formation, we performed the genome-wide transcriptional profiling in the flower buds of both male meiotic cytokinesis (mmc) mutant and its wild-type plants of brassica campestris l. ssp. chinensis, syn. b. rapa l. ssp. chinensis. cdna-amplified fragment length polymorphism (cdna-aflp) analysis showed that the mmc mutation resulted in changes in expression of a variety of genes. bcmf7, a ...200717653671
[genetic model analysis of leaf-weight traits in non-heading chinese cabbage (brassica campestris ssp. chinensis makino)].the mixed major-gene plus polygene inheritance model was used to analyze the genetics of leafblade-weight and petiole-weight trait in sixqiu017 of non-heading chinese cabbage. the results showed that the leafblade-weight trait was controlled by one pair of negative complete dominant major genes plus additive-dominant polygene (d-4). the additive effect in the major gene was 1.8991, the dominant effect was -1.8991; the additive effect in the polygene was -1.2934, and the dominant effect was 1.793 ...200717855268
salicylic acid alleviates the cadmium toxicity in chinese cabbages (brassica chinensis).to test the effects of salicylic acid (sa) on physiological changes of plants under cadmium stress one cultivar of chinese cabbage (brassica chinensis) cadmium resistance, changkenxiaobaicai (tolerance) were studied with pots cultures. like other organisms plants have adaptatives mechanisms whereby they are able to respond to both nutrient deficiency and toxicities. plants materials were originated from the vegetable market in wuhan, hubei china. the seeds were tested on the pot cultures in the ...200719090100
identification of flavonoids and hydroxycinnamic acids in pak choi varieties (brassica campestris l. ssp. chinensis var. communis) by hplc-esi-msn and nmr and their quantification by hplc-dad.twenty-eight polyphenols (11 flavonoid derivatives and 17 hydroxycinnamic acid derivatives) were detected in different cultivars of the chinese cabbage pak choi ( brassica campestris l. ssp. chinensis var. communis) by hplc-dad-esi-ms(n). kaempferol was found to be the major flavonoid in pak choi, glycosylated and acylated with different compounds. smaller amounts of isorhamnetin were also detected. a structural determination was carried out by (1)h and (13)c nmr spectroscopy for the main compou ...200717848079
phytotoxicity data safeguard the performance of the recipient plants in leachate irrigation.leachates from an operating and a closed landfill were examined for their phytotoxicity by seed germination/root elongation tests using seeds of brassica chinensis and lolium perenne. their ec50s ranged from 3% to 46% v/v, which varied remarkably with the operating status of the landfills. seedlings of twelve tree species were grown in pots, which were irrigated with landfill leachate at the ec50 levels, with tap water as control. no tree mortality or growth inhibition was observed after 90 days ...200716701930
ecotoxicity of a polycyclic aromatic hydrocarbon (pah)-contaminated soil.soil samples from a former cokery site polluted with polycyclic aromatic hydrocarbons (pahs) were assessed for their toxicity to terrestrial and aquatic organisms and for their mutagenicity. the total concentration of the 16 pahs listed as priority pollutants by the us environmental protection agency (us-epa) was 2634+/-241 mg/kgdw in soil samples. the toxicity of water-extractable pollutants from the contaminated soil samples was evaluated using acute (vibrio fischeri; microtox test, daphnia ma ...200717382389
[molecular cloning and characterization of bcmybogu, a novel member of the myb family involved in ogucms in brassica campestris ssp. chinensis].in the attempt to elucidate the molecular mechanism of cms. ogura cytoplasmic male sterile (ogucms) lines were obtained in chinese cabbage after interspecific hybridization between brassica. napus l. ogucms and b. campestris ssp. chinensis followed by recurrent backcross with b. campestris ssp. chinensis as the pollen donor. the cms lines were significantly characterized by the whitish anther and indehiscence of anther. the tapetal hypertrophy with excess vacuola-tion was the first observed defe ...200717548334
screening of pahs and ddts in sand and acrisols soil by a rapid solid-phase microalgal bioassay.previously we have demonstrated the rapid screening potential of a newly developed solid-phase microalgal bioassay with spiked sands. in this paper, we report further comparative results using both pahs and ddts spiked sands and field-collected acrisols soils. toxicity responses obtained from standard higher plant tests with three species of plants (lolium perenne, cynodon dactylon and brassica chinensis) were compared with those obtained from a cocktail of microalgae (selenastrum capricornutum, ...200717503178
antioxidant capacity and phenolic content of selected commercially available cruciferous vegetables.antioxidant activity, free radical scavenging activity and phenolic content of red cabbage (brassica oleracea var. capitata rubra), chinese cabbage (brassica rapa pekinensis var cylindrica), green cabbage (brassica oleracea var capitata), mustard cabbage (brassica juncea var rugosa) and chinese white cabbage (brassica rapa var chinensis), grown in malaysia, were evaluated. red cabbage had the highest antioxidant activity and phenolic content compared to the other cruciferous vegetables studied ( ...200722692190
free and bound phenolic compounds in leaves of pak choi (brassica campestris l. ssp. chinensis var. communis) and chinese leaf mustard (brassica juncea coss).eleven pak choi cultivars and two leaf mustard cultivars grown under field conditions in china were investigated for the free polyphenol content in their outer and inner leaves, as well as in their leaf blades and leaf stalks. in most cases, there were no significant differences between the hydroxycinnamic acid derivative and flavonoid derivative contents in the outer and inner leaves for the 13 cultivars. however, the contents of blades and stalks differed: hydroxycinnamic acids and flavonoids ...200826047268
impact of fermentation on phenolic compounds in leaves of pak choi (brassica campestris l. ssp. chinensis var. communis) and chinese leaf mustard (brassica juncea coss).four different cultivars of chinese brassica vegetables (two pak choi cultivars and two chinese leaf mustard cultivars) were fermented according to a traditional chinese method called pickling. the plant material was investigated before and after the fermentation procedure to determine the qualitative and quantitative changes in its polyphenols. a detailed description of the identified phenolic compounds of leaf mustard by hplc-esi-ms(n) is presented here for the first time, including hydroxycin ...200818078315
bioavailability of chemical pollutants in contaminated soils and pitfalls of chemical analyses in hazard assessment.decision-making for remediation of industrial wastelands are still based on the concentrations of pollutants of concern measured in soils. in this work, two soils polluted by polycyclic aromatic hydrocarbons (pahs) and metals were investigated for their toxicity on earthworms (eisenia fetida), collembolae (folsomia candida), and higher plants (brassica chinensis, lactucca sativa and avena sativa) in order to study the relationships between chemical contamination and biological effects. although ...200818561306
the polygalacturonase gene bcmf2 from brassica campestris is associated with intine development.brassica campestris male fertility 2 (bcmf2) is a putative polygalacturonase (pg) gene previously isolated from the flower bud of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). this gene was found to be expressed specifically in tapetum and pollen after the tetrad stage of anther development. antisense rna technology was used to study the function of bcmf2 in chinese cabbage. scanning and transmission electron microscopy revealed that there were defo ...200819039102
[risk assessment of pig manure cu-contamination of black soil in northeast china].copper (cu) is massively used as feed additives in intensive farms, and the discharge and utilization of livestock wastes may cause certain environmental problems. in this paper, a cultivated black soil in northeast china was sampled, and added with pig manures that contained different concentration of cu to simulate the vegetable soil under different years of pig manure fertilization. with this soil, a pot experiment was conducted to study the effects of soil cu accumulation via the application ...200819288734
accumulation properties of cadmium in a selected vegetable-rotation system of southeastern china.a rotation experiment was conducted in a greenhouse with three vegetable crops on red yellowish soil (rys) and silt loamy soil (sls) to study cd accumulation in pak choi ( brassica chinensis l.), tomato ( lycopersicon esculentum), and radish ( raphanus sativus l.). critical cd concentrations in the two soils were evaluated for these vegetables based on human dietary toxicity. cadmium was added as cd(no 3) 2 at a rate of 0-7.00 mg cd kg (-1) soil. shoot growth was not inhibited by cd except for r ...200818593175
[effects of silicon on flowering chinese cabbage's anthracnose occurence, flower stalk formation, and silicon uptake and accumulation].different concentrations of silicon (si) were applied to flowering chinese cabbbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) to study their effects on the flowering chinese cabbage's anthracnose occurrence, flower stalk formation, and si uptake and accumulation. the results indicated that si could obviously control the occurrence of anthracnose, and the effect was genotype-dependant. the plants of susceptible cultivar applied with 2.5 mmol l(-1) si and those of resistant c ...200818655585
bcltp, a novel lipid transfer protein in brassica chinensis, may secrete and combine extracellular cam.lipid transfer proteins in plants are believed to be involved in many processes of cell physiology and development. in this work, a full-length cdna encoding a novel lipid transfer protein, designated bcltp was isolated from brassica chinensis. at least two copies of bcltp are present in whole genome of b. chinensis, and its transcripts preferably accumulate in second-year organs, implying its role in reproductive growth stage. the 118 amino acid sequence deduced from a 354 bp open reading frame ...200817891402
bcmf13, a new reproductive organ-specific gene from brassica rapa. ssp. chinensis, affects pollen development.a transcript-derived fragment (genbank accession number dn237920.1) accumulated in the wild-type flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) was isolated and further investigated. the full length dna and cdna of the fragment were cloned by rapid amplification of cdna ends. the gene, bcmf13, encodes a protein of 73 amino acids and is interrupted by an intron of 106 bp (genbank accession number ef158459). southern blot analysis reveale ...200817387632
[effects of cadmium on the growth and nitrogen metabolism in brassica chinensis].hydroponic culture was conducted to study the effect of cd on the growth, metal accumulation and nitrogen metabolism in brassica chinensis. the enzymatic activities of nitrogen metabolism including nitrate reductase (nr), glutamine synthetase (gs) and gs-transferase as well as the concentrations of chlorophyll, free proline, soluble protein, no3(-) -n, nh4+ -n and nutrients in brassica chinensis were determined. results indicated that the addition of cd reduced the content of the soluble protein ...200818613528
effects of field application of phosphate fertilizers on the availability and uptake of lead, zinc and cadmium by cabbage (brassica chinensis l.) in a mining tailing contaminated soil.a field trial was conducted to evaluate the reduction of bioavailability of heavy metals including lead (pb), zinc (zn) and cadmium (cd) in a soil contaminated by mining tailings in shaoxing, zhejiang, china. three commercial phosphate (p) fertilizers including phosphate rock (pr), calcium magnesium phosphate (cmp), and single superphosphate (ssp) were applied to the plot at three p application rates, 50, 300, and 500 g/m2 with 9 treatments and control (ck). plants, water soluble and exchangeabl ...200819143319
characterization and functional analysis of a novel pcp gene bcmf5 from chinese cabbage (brassica campestris l. ssp. chinensis makino).brassica campestris male fertile 5 (bcmf5), a novel member of the pollen coat protein class a (pcp-a) gene family, was identified from brassica campestris l. ssp. chinensis makino (chinese cabbage-pak-choi). temporal and spatial expression analysis showed that bcmf5 is a late-expressed pcp gene related to the process of determining pollen fertility. functional analysis by hairpin rna (hprna)-mediated rna interference also showed that the expression of bcmf5 is inhibited, which resulted in the lo ...200817945379
regulation of nitrate reductase by nitric oxide in chinese cabbage pakchoi (brassica chinensis l.).nitrate reductase (nr), a committed enzyme in nitrate assimilation, involves generation of nitric oxide (no) in plants. here we show that the nr activity was significantly enhanced by the addition of no donors sodium nitroprusside (snp) and nonoate (diethylamine nonoate sodium) to the culturing solution, whereas it was decreased by no scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (cptio). interestingly, both no gas and snp directly enhanced but cptio inhibited the ...200818028279
characterization of a putative pollen-specific arabinogalactan protein gene, bcmf8, from brassica campestris ssp. chinensis.the bcmf8 (brassica campestris male fertility 8) gene, possessing the features of 'classical' arabinogalactan protein (agp) was isolated from brassica campestris l. ssp. chinensis, makino syn. b. rapa l. ssp. chinensis. this gene was highly abundant in the fertile flower buds but silenced in the sterile ones of genic male sterile a/b line ('zubajh97-01a/b') in b. campestris. expression patterns analysis suggested bcmf8 was a pollen-specific gene, whose transcript started to be expressed at the u ...200817786584
transcriptional differences between the male-sterile mutant bcms and wild-type brassica campestris ssp. chinensis reveal genes related to pollen development.a novel male-sterile mutant which lacks mature pollen, brassisa campestris male sterile (bcms), was identified in brassica campestris l. ssp. chinensis makino (syn. b. rapa ssp. chinensis). genetic analysis revealed that bcms was controlled by a single recessive mutation locus. genome-wide transcriptional profiling was performed on the flower buds of both the bcms mutant and the wild-type from which it originated, and profiling analysis indicated that there were numerous changes in gene expressi ...200818426481
functional analysis of a pollen-expressed polygalacturonase gene bcmf6 in chinese cabbage (brassica campestris l. ssp. chinensis makino).in our earlier work, a pollen-expressed polygalacturonase gene bcmf6 was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino) by cdna-amplified fragment length polymorphism (cdna-aflp) transcript profiling and rapid amplification of cdna ends (race). to unravel the biological function of bcmf6 gene, the antisense fragment from the bcmf6 gene with a9 promoter and camv35s promoter was transferred into flowering chinese cabbage (b. campestris ssp. chinensis var ...200818415101
phaedon cochleariae (f.) performance on different crucifer varieties with different glucosinolate profiles.the mustard leaf beetle, phaedon cochleariae (f.) (coleoptera: chrysomelidae), is a serious insect pest of cruciferous crops, known especially in europe. feeding performances of p. cochleariae on six varieties of crucifer crops (sinapis alba, flata and litember; raphanus sativus, münchener bier and runder schwarzer winter; and brassica chinensis, joi choi and black behi) was investigated. the larval performance, measured by weight increase within seven days, was significantly different among six ...200819226796
growth and elemental accumulation of plants grown in acidic soil amended with coal fly ash-sewage sludge co-compost.a greenhouse experiment was conducted to evaluate the growth and heavy-metal accumulation of brassica chinensis and agropyron elongatum in 10 and 25% ash-sludge co-compost (asc)--amended loamy acidic soil (ph 4.51) at two different application rates: 20% and 40% (v/v). soil ph increased, whereas electrical conductivity decreased with the amendment of asc to soil. bioavailable cu, zn, and mn contents of asc-amended soil decreased, whereas ni, pb, and b contents increased. concentrations of bioava ...200919294455
characterization of a male sterile related gene bcmf15 from brassica campestris ssp. chinensis.data from cdna-aflp analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of brassica campestris l. ssp. chinensis makino, syn. b. rapa l. ssp. chinensis, indicated that mutation of the mmc gene resulted in changes in expression of a variety of genes. a transcript-derived fragment specifically accumulated in the wild-type flower buds was isolated, and the corresponding full-length cdna and dna was subsequentl ...200918034318
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