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herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in c4-fibre plants.the enrichment of nitrogen-fixing bacteria from the c4-fibre plants, spartina pectinata, miscanthus sinensis, miscanthus sacchariflorus and pennisetum purpureum, with nitrogen-free semi-solid media led to the isolation of herbaspirillum-like strains among other diazotrophic bacteria. on the basis of physiological properties, phylogenetic analysis comparing 16s rdna sequences and dna-dna hybridization experiments of chromosomal dna the new isolates could be grouped together in a new species with ...200111211253
endophytic root colonization of gramineous plants by herbaspirillum frisingense.herbaspirillum frisingense is a diazotrophic betaproteobacterium isolated from c4-energy plants, for example miscanthus sinensis. to demonstrate endophytic colonization unequivocally, immunological labeling techniques using monospecific polyclonal antibodies against two h. frisingense strains and green fluorescent protein (gfp)-fluorescence tagging were applied. the polyclonal antibodies enabled specific in situ identification and very detailed localization of h. frisingense isolates mb11 and gs ...200818761671
[diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-vetiver zizanioides].vetiver zizanioides is a perennial grass of the poaceae family, known of its silage, soil and water conservation role. the aim of the study was to collect and identify the resources of the nitrogen-fixing bacteria associated with vetiver zizanioides.200920112669
herbaspirillum psychrotolerans sp. nov., a member of the family oxalobacteraceae from a glacier forefield.a novel psychrotolerant, gram-negative, shiny white, curved-rod-shaped, facultatively anaerobic bacterium pb1(t) was isolated from a soil sample collected from a glacier forefield of the larsemann hills, east antarctica. isolate pb1(t) has catalase and low urease activity and hydrolyses gelatin and starch. strain pb1(t) is able to grow between -5 °c and 30 °c with optimum growth at 14-20 °c. glycerol, dl-arabinose, d-xylose, d-galactose, d-fructose, d-lyxose, d-fucose and potassium gluconate are ...201323456802
paraherbaspirillum soli gen. nov., sp. nov. isolated from soil.a bacterial strain, designated js5-2(t), was isolated from soil collected from jeju island, republic of korea. the cells of the strain were gram-negative, nonspore forming, catalase- and oxidase-positive, aerobic, nonmotile and rod-shaped. strain js5-2(t) exhibited 96.2-97.2, 95.1-96.3, and 95.4-95.8% 16s rrna gene sequence similarities to the genera herbaspirillum, oxalicibacterium, and herminiimonas, respectively. the highest sequence similarities were with herbaspirillum autotrophicum iam 149 ...201323625231
the genome of the endophytic bacterium h. frisingense gsf30(t) identifies diverse strategies in the herbaspirillum genus to interact with plants.the diazotrophic, bacterial endophyte herbaspirillum frisingense gsf30(t) has been identified in biomass grasses grown in temperate climate, including the highly nitrogen-efficient grass miscanthus. its genome was annotated and compared with related herbaspirillum species from diverse habitats, including h. seropedicae, and further well-characterized endophytes. the analysis revealed that herbaspirillum frisingense lacks a type iii secretion system that is present in some related herbaspirillum ...201323825472
root ethylene signalling is involved in miscanthus sinensis growth promotion by the bacterial endophyte herbaspirillum frisingense gsf30(t).the bacterial endophyte herbaspirillum frisingense gsf30(t) is a colonizer of several grasses grown in temperate climates, including the highly nitrogen-efficient perennial energy grass miscanthus. inoculation of miscanthus sinensis seedlings with h. frisingense promoted root and shoot growth but had only a minor impact on nutrient concentrations. the bacterium affected the root architecture and increased fine-root structures. although h. frisingense has the genetic requirements to fix nitrogen, ...201324043849
simplified and representative bacterial community of maize roots.plant-associated microbes are important for the growth and health of their hosts. as a result of numerous prior studies, we know that host genotypes and abiotic factors influence the composition of plant microbiomes. however, the high complexity of these communities challenges detailed studies to define experimentally the mechanisms underlying the dynamics of community assembly and the beneficial effects of such microbiomes on plant hosts. in this work, from the distinctive microbiota assembled ...201728275097
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