| burkholderia multivorans acts as an antagonist against the growth of burkholderia pseudomallei in soil. | burkholderia multivorans nki379 has an antagonistic effect against the growth of b. pseudomallei, which we have demonstrated here using agar diffusion tests and a transwell system. bacterial representatives were isolated from agricultural crop soil and mixed to construct a partial bacterial community structure that based on the results of reproducible patterns following pcr-dgge (denaturing gradient gel electrophoresis) analysis from total soil chromosomes. the antagonistic effect of b. multivor ... | 2011 | 21752084 |
| Screening microorganisms for insulin binding reveals binding by Burkholderia multivorans and Burkholderia cenocepacia and novel attachment of insulin to Aeromonas salmonicida via the A-layer. | Exposure to microorganisms is considered an environmental factor which can contribute to Type 1 diabetes. Insulin binding proteins on microorganisms may induce production of antibodies which can react with the human insulin receptor (HIR) with possible consequences in developing a diabetic autoimmune response against HIR and insulin. The interaction of insulin with microorganisms was studied by screening forty-five microbial species for their ability to bind insulin. Binding assays were performe ... | 2011 | 22171975 |
| characterization of the poly-β-1,6-n-acetylglucosamine polysaccharide component of burkholderia biofilms. | we demonstrated the production of poly-β-1,6-n-acetylglucosamine (pnag) polysaccharide in the biofilms of burkholderia multivorans, burkholderia vietnamiensis, burkholderia ambifaria, burkholderia cepacia, and burkholderia cenocepacia using an immunoblot assay for pnag. these results were confirmed by further studies, which showed that the pnag hydrolase, dispersin b, eliminated immunoreactivity of extracts from the species that were tested (b. cenocepacia and b. multivorans). dispersin b also i ... | 2011 | 21984237 |
| Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans. | A major challenge to clinical therapy of Burkholderia cepacia complex (Bcc) pulmonary infections is their innate resistance to a broad range of antimicrobials, including polycationic agents such as aminoglycosides, polymyxins, and cationic peptides. To identify genetic loci associated with this phenotype, a transposon mutant library was constructed in B. multivorans ATCC 17616 and screened for increased susceptibility to polymyxin B. Compared to the parent strain, mutant 26D7 exhibited 8- and 16 ... | 2012 | 22006009 |
| cepacia syndrome in a cystic fibrosis patient colonised with burkholderia multivorans. | | 2011 | 22715269 |
| immunoproteomic analysis of proteins expressed by two related pathogens, burkholderia multivorans and burkholderia cenocepacia, during human infection. | burkholderia cepacia complex (bcc) is an opportunistic bacterial pathogen that causes chronic infections in people with cystic fibrosis (cf). it is a highly antibiotic resistant organism and bcc infections are rarely cleared from patients, once they are colonized. the two most clinically relevant species within bcc are burkholderia cenocepacia and burkholderia multivorans. the virulence of these pathogens has not been fully elucidated and the virulence proteins expressed during human infection h ... | 2013 | 24260482 |
| comparative metabolic systems analysis of pathogenic burkholderia. | burkholderia cenocepacia and burkholderia multivorans are opportunistic drug-resistant pathogens that account for the majority of burkholderia cepacia complex infections in cystic fibrosis patients and also infect other immunocompromised individuals. while they share similar genetic compositions, b. cenocepacia and b. multivorans exhibit important differences in pathogenesis. we have developed reconciled genome-scale metabolic network reconstructions of b. cenocepacia j2315 and b. multivorans at ... | 2014 | 24163337 |
| identification of host-immune response protein candidates in the sera of human oral squamous cell carcinoma patients. | one of the most common cancers worldwide is oral squamous cell carcinoma (oscc), which is associated with a significant death rate and has been linked to several risk factors. notably, failure to detect these neoplasms at an early stage represents a fundamental barrier to improving the survival and quality of life of oscc patients. in the present study, serum samples from oscc patients (n = 25) and healthy controls (n = 25) were subjected to two-dimensional gel electrophoresis (2-de) and silver ... | 2014 | 25272005 |
| discovery of an l-fucono-1,5-lactonase from cog3618 of the amidohydrolase superfamily. | a member of the amidohydrolase superfamily, bmulj_04915 from burkholderia multivorans, of unknown function was determined to hydrolyze a series of sugar lactones: l-fucono-1,4-lactone, d-arabino-1,4-lactone, l-xylono-1,4-lactone, d-lyxono-1,4-lactone, and l-galactono-1,4-lactone. the highest activity was shown for l-fucono-1,4-lactone with a k(cat) value of 140 s(-1) and a k(cat)/k(m) value of 1.0 × 10(5) m(-1) s(-1) at ph 8.3. the enzymatic product of an adjacent l-fucose dehydrogenase, bmulj_0 ... | 2012 | 23214453 |
| can early burkholderia cepacia complex infection in cystic fibrosis be eradicated with antibiotic therapy? | organisms of the burkholderia cepacia complex (bcc) are important pathogens in cystic fibrosis (cf). the majority of those who acquire bcc develop chronic infection but it can also result in rapid decline in a significant minority. in addition, chronic infection with burkholderia cenocepacia in particular is regarded as a contraindication to lung transplantation in many units. whilst aggressive antibiotic therapy is employed in cf to eradicate pseudomonas aeruginosa before infection becomes irre ... | 2011 | 22919584 |
| draft genome sequence determination for cystic fibrosis and chronic granulomatous disease burkholderia multivorans isolates. | burkholderia multivorans is a gram-negative bacterium and a member of the burkholderia cepacia complex, which is frequently associated with respiratory infections in people with cystic fibrosis (cf) and chronic granulomatous disease (cgd). we are reporting the genome sequences of 4 b. multivorans strains, 2 from cf patients and 2 from cgd patients. | 2012 | 23105085 |
| stress conditions triggering mucoid morphotype variation in burkholderia species and effect on virulence in galleria mellonella and biofilm formation in vitro. | burkholderia cepacia complex (bcc) bacteria are opportunistic pathogens causing chronic respiratory infections particularly among cystic fibrosis patients. during these chronic infections, mucoid-to-nonmucoid morphotype variation occurs, with the two morphotypes exhibiting different phenotypic properties. here we show that in vitro, the mucoid clinical isolate burkholderia multivorans d2095 gives rise to stable nonmucoid variants in response to prolonged stationary phase, presence of antibiotics ... | 2013 | 24358195 |
| genomic characterization of jg068, a novel virulent podovirus active against burkholderia cenocepacia. | as is true for many other antibiotic-resistant gram-negative pathogens, members of the burkholderia cepacia complex (bcc) are currently being assessed for their susceptibility to phage therapy as an antimicrobial treatment. the objective of this study was to perform genomic and limited functional characterization of the novel bcc phage jg068 (vb_bcep_jg068). | 2013 | 23978260 |
| localization of burkholderia cepacia complex bacteria in cystic fibrosis lungs and interactions with pseudomonas aeruginosa in hypoxic mucus. | the localization of burkholderia cepacia complex (bcc) bacteria in cystic fibrosis (cf) lungs, alone or during coinfection with pseudomonas aeruginosa, is poorly understood. we performed immunohistochemistry for bcc and p. aeruginosa bacteria on 21 coinfected or singly infected cf lungs obtained at transplantation or autopsy. parallel in vitro experiments examined the growth of two bcc species, burkholderia cenocepacia and burkholderia multivorans, in environments similar to those occupied by p. ... | 2014 | 25156735 |
| development of a multiple-locus variable-number tandem-repeat typing scheme for genetic fingerprinting of burkholderia cenocepacia and application to nationwide epidemiological analysis. | organisms of the burkholderia cepacia complex are especially important pathogens in cystic fibrosis (cf), with a propensity for patient-to-patient spread and long-term respiratory colonization. b. cenocepacia and burkholderia multivorans account for the majority of infections in cf, and major epidemic clones have been recognized throughout the world. the aim of the present study was to develop and evaluate a multilocus variable-number tandem-repeat (vntr) analysis (mlva) scheme for b. cenocepaci ... | 2014 | 25411181 |
| analysis of changes in diversity and abundance of the microbial community in a cystic fibrosis patient over a multiyear period. | the evolution of pulmonary disease in cystic fibrosis (cf) usually begins when bacteria get trapped in mucus in the lungs and become established as a chronic infection. while most cf patients experience periods of stability, pulmonary exacerbations (pes) can occur multiple times per year and result in permanent damage to the lungs. little is known of the shift from a period of stability to a pe, but this shift is likely to be attributed to changes in the bacterial community. here, we identified ... | 2014 | 25392361 |
| mechanical homogenization increases bacterial homogeneity in sputum. | sputum obtained from patients with cystic fibrosis (cf) is highly viscous and often heterogeneous in bacterial distribution. adding dithiothreitol (dtt) is the standard method for liquefaction prior to processing sputum for molecular detection assays. to determine if dtt treatment homogenizes the bacterial distribution within sputum, we measured the difference in mean total bacterial abundance and abundance of burkholderia multivorans between aliquots of dtt-treated sputum samples with and witho ... | 2014 | 24759710 |
| fosmidomycin decreases membrane hopanoids and potentiates the effects of colistin on burkholderia multivorans clinical isolates. | burkholderia cepacia complex (bcc) pulmonary infections in people living with cystic fibrosis (cf) are difficult to treat because of the extreme intrinsic resistance of most isolates to a broad range of antimicrobials. fosmidomycin is an antibacterial and antiparasitic agent that disrupts the isoprenoid biosynthesis pathway, a precursor to hopanoid biosynthesis. hopanoids are involved in membrane stability and contribute to polymyxin resistance in bcc bacteria. checkerboard mic assays determined ... | 2014 | 24957830 |
| draft genome sequences of two burkholderia multivorans sequential isolates from a chronic lung infection of a cystic fibrosis patient. | burkholderia multivorans belongs to the burkholderia cepacia complex, which comprises opportunistic pathogens infecting cystic fibrosis (cf) patients. here, we report the genome sequences and annotations of two sequential b. multivorans clinical isolates (d2095 and d2214) displaying different traits. the differences in the genomic contents of these isolates may provide clues regarding the evolution of b. multivorans within the airways of a cf patient. | 2015 | 25676757 |
| pcr detection of burkholderia multivorans in water and soil samples. | although semi-selective growth media have been developed for the isolation of burkholderia cepacia complex bacteria from the environment, thus far burkholderia multivorans has rarely been isolated from such samples. because environmental b. multivorans isolates mainly originate from water samples, we hypothesized that water rather than soil is its most likely environmental niche. the aim of the present study was to assess the occurrence of b. multivorans in water samples from flanders (belgium) ... | 2016 | 27514367 |
| activity of tobramycin against cystic fibrosis isolates of burkholderia cepacia complex grown as biofilms. | pulmonary infection with burkholderia cepacia complex in cystic fibrosis (cf) patients is associated with more-rapid lung function decline and earlier death than in cf patients without this infection. in this study, we used confocal microscopy to visualize the effects of various concentrations of tobramycin, achievable with systemic and aerosolized drug administration, on mature b. cepacia complex biofilms, both in the presence and absence of cf sputum. after 24 h of growth, biofilm thickness wa ... | 2015 | 26503664 |
| genomic sequence of burkholderia multivorans nki379, a soil bacterium that inhibits the growth of burkholderia pseudomallei. | burkholderia multivorans nki379 is a soil bacterium that exhibits an antagonistic effect against the growth of burkholderia pseudomallei, the causative agent of the infectious disease melioidosis. we report the draft genomic sequence of b. multivorans nki379, which has a g+c content of 67% and 5,203 candidate protein-encoding genes. | 2015 | 26586873 |
| long-term evolution of burkholderia multivorans during a chronic cystic fibrosis infection reveals shifting forces of selection. | burkholderia multivorans is an opportunistic pathogen capable of causing severe disease in patients with cystic fibrosis (cf). patients may be chronically infected for years, during which the bacterial population evolves in response to unknown forces. here we analyze the genomic and functional evolution of a b. multivorans infection that was sequentially sampled from a cf patient over 20 years. the population diversified into at least four primary, coexisting clades with distinct evolutionary dy ... | 2016 | 27822534 |
| polyphasic characterisation of burkholderia cepaciacomplex species isolated from children with cystic fibrosis. | cystic fibrosis (cf) patients with burkholderia cepacia complex (bcc) pulmonary infections have high morbidity and mortality. the aim of this study was to compare different methods for identification of bcc species isolated from paediatric cf patients. oropharyngeal swabs from children with cf were used to obtain isolates of bcc samples to evaluate six different tests for strain identification. conventional (cpt) and automatised (apt) phenotypic tests, polymerase chain reaction (pcr)-reca, restr ... | 2016 | 26814642 |
| the effect of sinus surgery with intensive follow-up on pathogenic sinus bacteria in patients with cystic fibrosis. | most patients with cystic fibrosis (cf) have chronic rhinosinusitis; their sinuses are often colonized with bacteria that can initiate and maintain deleterious pulmonary infections. theoretically, eradication of the sinus bacteria should reduce the frequency of lung infections and thereby reduce pulmonary morbidity. this article addressed whether bacteria in cf sinuses are eligible for eradication by sinus surgery and postoperative treatment. | 2013 | 23406585 |
| quorum sensing protects pseudomonas aeruginosa against cheating by other species in a laboratory coculture model. | many species of bacteria use a cell-cell communication system called quorum sensing (qs) to coordinate group activities. qs systems frequently regulate the production of exoproducts. some of these products, such as proteases, are "public goods" that are shared among the population and vulnerable to cheating by nonproducing members of the population. because the qs system of the opportunistic pathogen pseudomonas aeruginosa regulates several public goods, it can serve as a model for studying coop ... | 2015 | 26195596 |
| denitrification by cystic fibrosis pathogens - stenotrophomonas maltophilia is dormant in sputum. | chronic pseudomonas aeruginosa lung infection is the most severe complication for cystic fibrosis (cf) patients. infected endobronchial mucus of cf patients contains anaerobic zones mainly due to the respiratory burst of polymorphonuclear leukocytes. we have recently demonstrated ongoing denitrification in sputum from patients infected with p. aeruginosa. therefore we aimed to investigate, whether the pathogenicity of several known cf pathogens is correlated to their ability to perform denitrifi ... | 2015 | 25441256 |
| determinants of antibacterial spectrum and resistance potential of the elongation factor g inhibitor argyrin b in key gram-negative pathogens. | argyrins are natural products with antibacterial activity against gram-negative pathogens, such as pseudomonas aeruginosa, burkholderia multivorans, and stenotrophomonas maltophilia we previously showed that argyrin b targets elongation factor g (fusa). here, we show that argyrin b activity against p. aeruginosa pao1 (mic = 8 μg/ml) was not affected by deletion of the mexab-oprm, mexxy-oprm, mexcd-oprj, or mexef-oprn efflux pump. however, argyrin b induced expression of mexxy, causing slight but ... | 2017 | 28096160 |
| combination antimicrobial susceptibility testing of burkholderia cepacia complex: significance of species. | the burkholderia cepacia complex (bcc) is notorious for the life-threatening pulmonary infections it causes in patients with cystic fibrosis. the multidrug-resistant nature of bcc and differing infective bcc species make the design of appropriate treatment regimens challenging. previous synergy studies have failed to take account of the species of bcc isolates. etest methodology was used to facilitate minimum inhibitory concentration (mic) and antimicrobial combination testing on 258 isolates of ... | 2016 | 27665523 |
| identification of synergists that potentiate the action of polymyxin b against burkholderia cenocepacia. | burkholderia cenocepacia and other members of the burkholderia cepacia complex (bcc) are highly multidrug-resistant bacteria that cause severe pulmonary infections in patients with cystic fibrosis. a screen of 2686 compounds derived from marine organisms identified molecules that could synergise with polymyxin b (pmb) to inhibit the growth of b. cenocepacia. at 1 μg/ml, five compounds synergised with pmb and inhibited the growth of b. cenocepacia by ≥70% compared with growth in pmb alone. follow ... | 2015 | 26187366 |
| antibiotic resistance in burkholderia species. | the genus burkholderia comprises metabolically diverse and adaptable gram-negative bacteria, which thrive in often adversarial environments. a few members of the genus are prominent opportunistic pathogens. these include burkholderia mallei and burkholderia pseudomallei of the b. pseudomallei complex, which cause glanders and melioidosis, respectively. burkholderia cenocepacia, burkholderia multivorans, and burkholderia vietnamiensis belong to the burkholderia cepacia complex and affect mostly c ... | 2016 | 27620956 |
| accurate identification of members of the burkholderia cepacia complex in cystic fibrosis sputum. | the burkholderia cepacia complex (bcc) is a group of closely related species which includes opportunistic pathogens causing chronic respiratory infections in immunocompromised patients, or individuals affected by cystic fibrosis (cf). other burkholderia species causing infection in the cf population are burkholderia gladioli and burkholderia pseudomallei. traditional phenotypic analyses have been demonstrated to be inadequate for reliable identifications of isolates of bcc and b. gladioli. a pan ... | 2016 | 26671758 |
| inhibition of co-colonizing cystic fibrosis-associated pathogens by pseudomonas aeruginosa and burkholderia multivorans. | cystic fibrosis (cf) is a recessive genetic disease characterized by chronic respiratory infections and inflammation causing permanent lung damage. recurrent infections are caused by gram-negative antibiotic-resistant bacterial pathogens such as pseudomonas aeruginosa, burkholderia cepacia complex (bcc) and the emerging pathogen genus pandoraea. in this study, the interactions between co-colonizing cf pathogens were investigated. both pandoraea and bcc elicited potent pro-inflammatory responses ... | 2014 | 24790091 |
| a burkholderia cenocepacia gene encoding a non-functional tyrosine phosphatase is required for the delayed maturation of the bacteria-containing vacuoles in macrophages. | burkholderia cenocepacia infects patients with cystic fibrosis. we have previously shown that b. cenocepacia can survive in macrophages within membrane vacuoles [b. cenocepacia-containing vacuoles (bccvs)] that preclude fusion with the lysosome. the bacterial factors involved in b. cenocepacia intracellular survival are not fully elucidated. we report here that deletion of bcam0628, encoding a predicted low molecular weight protein tyrosine phosphatase (lmw-ptp) that is restricted to b. cenocepa ... | 2014 | 24728272 |
| efficacy of the biocide steri-7 against the common gram-negative bacterial pathogens (burkholderia cenocepacia, burkholderia gladioli, burkholderia multivorans, pseudomonas aeruginosa and stenotrophomonas maltophilia) associated with cystic fibrosis (cf). | | 2013 | 23888610 |
| burkholderia multivorans survival and trafficking within macrophages. | cystic fibrosis (cf) patients are at great risk of opportunistic lung infection, particularly by members of the burkholderia cepacia complex (bcc). this group of bacteria can cause damage to the lung tissue of infected patients and are difficult to eradicate due to their high levels of antibiotic resistance. although the highly virulent burkholderia cenocepacia has been the focus of virulence research for the past decade, burkholderia multivorans is emerging as the most prevalent bcc species inf ... | 2013 | 23105020 |
| overcoming an extremely drug resistant (xdr) pathogen: avibactam restores susceptibility to ceftazidime for burkholderia cepacia complex isolates from cystic fibrosis patients. | burkholderia multivorans is a significant health threat to persons with cystic fibrosis (cf). infections are difficult to treat as this pathogen is inherently resistant to multiple antibiotics. susceptibility testing of isolates obtained from cf respiratory cultures revealed that single agents selected from different antibiotic classes were unable to inhibit growth. however, all isolates were found to be susceptible to ceftazidime when combined with the novel non-β-lactam β-lactamase inhibitor, ... | 2017 | 28264560 |
| exploring the role of the ω-loop in the evolution of ceftazidime resistance in the pena β-lactamase from burkholderia multivorans, an important cystic fibrosis pathogen. | the unwelcome evolution of resistance to the advanced generation cephalosporin antibiotic, ceftazidime is hindering the effective therapy of burkholderia cepacia complex (bcc) infections. regrettably, bcc organisms are highly resistant to most antibiotics, including polymyxins; ceftazidime and trimethoprim-sulfamethoxazole are the most effective treatment options. unfortunately, resistance to ceftazidime is increasing and posing a health threat to populations susceptible to bcc infection. we fou ... | 2017 | 27872073 |
| bacterial proteases and haemostasis dysregulation in the cf lung. | pathogenic bacteria which chronically colonise the cystic fibrosis (cf) lung produce a number of virulence determinants, including distinct proteolytic activities. the potential role bacterial proteases play on haemostatic dysregulation within the cf lung is, however, poorly defined, despite haemoptysis being a common complication in cf. | 2017 | 27839953 |
| cd4+ t cell epitopes of flic conserved between strains of burkholderia: implications for vaccines against melioidosis and cepacia complex in cystic fibrosis. | burkholderia pseudomallei is the causative agent of melioidosis characterized by pneumonia and fatal septicemia and prevalent in southeast asia. related burkholderia species are strong risk factors of mortality in cystic fibrosis (cf). the b. pseudomallei flagellar protein flic is strongly seroreactive and vaccination protects challenged mice. we assessed b. pseudomallei flic peptide binding affinity to multiple hla class ii alleles and then assessed cd4 t cell immunity in hla class ii transgeni ... | 2014 | 25392525 |
| burkholderia species infections in patients with cystic fibrosis in british columbia, canada. 30 years' experience. | we have been collecting burkholderia species bacteria from patients with cystic fibrosis (cf) for the last 30 years. during this time, our understanding of their multispecies taxonomy and infection control has evolved substantially. | 2015 | 25474359 |
| synthesis of the tetrasaccharide outer core fragment of burkholderia multivorans lipooligosaccharide. | the first synthesis of the outer core fragment of burkholderia multivorans lipooligosaccharide [β-d-glc-(1→3)-α-d-galnac-(1→3)-β-d-galnac-(1→3)-l-rha] as α-allyl tetrasaccharide was accomplished. the glycosylations involving galnac units were studied in depth testing them under several conditions. this allowed the building of both the α- and the β-configured glycosidic bonds by employing the same galnac glycosyl donor, thus considerably shortening the total number of synthetic steps. the target ... | 2015 | 24933233 |
| biosafety and colonization of burkholderia multivorans ws-fj9 and its growth-promoting effects on poplars. | burkholderia cepacia complex (bcc) is a group of bacteria with conflicting biological characteristics, which make them simultaneously beneficial and harmful to humans. they have been exploited for biocontrol, bioremediation, and plant growth promotion. however, their capacity as opportunistic bacteria that infect humans restricts their biotechnological applications. therefore, the risks of using these bacteria should be assessed. in this study, burkholderia multivorans ws-fj9 originally isolated ... | 2013 | 24092012 |
| an outbreak of burkholderia multivorans beyond cystic fibrosis patients. | strains of the burkholderia cepacia complex (bcc) are opportunistic pathogens capable of causing serious infection in cystic fibrosis patients. recently we identified a suspected outbreak of infection with bcc strains at the university hospital olomouc. seventy-four bcc strains were isolated from 52 patients, most of whom (n = 48) did not suffer from cystic fibrosis. most frequently (n = 46) burkholderia multivorans was isolated and 24 (52.2%) of these strains were clonal. fifteen of these strai ... | 2013 | 23706672 |
| rapid emergence of a ceftazidime-resistant burkholderia multivorans strain in a cystic fibrosis patient. | burkholderia multivorans poses a serious health threat to cystic fibrosis patients due to innate resistance to multiple antibiotics and acquisition of resistance to a range of antibiotics due to the frequent use of antibiotics to treat chronic infections. monitoring antibiotic susceptibility is crucial to managing patient care. we identified the rapid emergence of a ceftazidime-resistant strain in a single patient within four days during a hospitalization for treatment of an exacerbation. | 2013 | 23478130 |
| mannitol promotes adherence of an outbreak strain of burkholderia multivorans via an exopolysaccharide-independent mechanism that is associated with upregulation of newly identified fimbrial and afimbrial adhesins. | burkholderia multivorans, a member of the burkholderia cepacia complex (bcc), is an important pathogen of the cystic fibrosis (cf) lung. mannitol, approved as an inhaled osmolyte therapy for use in cf patients, promotes exopolysaccharide (eps) production by the bcc. in the present study, we investigated the role of mannitol-induced eps in the adherence of b. multivorans. we report that mannitol promoted adherence of two representative b. multivorans strains. however, whilst this enhanced adheren ... | 2013 | 23378576 |
| inhibition of burkholderia multivorans adhesion to lung epithelial cells by bivalent lactosides. | burkholderia cepacia complex (bcc) is an opportunistic pathogen in cystic fibrosis patients which is inherently resistant to antimicrobial agents. the mechanisms of attachment and pathogenesis of bcc, a group of 17 species, are poorly understood. the most commonly identified bcc species in newly colonised patients, burkholderia multivorans, continues to be acquired from the environment. development of therapies which can prevent or reduce the risk of colonization on exposure to bcc in the enviro ... | 2012 | 22922277 |
| extensive cultivation of soil and water samples yields various pathogens in patients with cystic fibrosis but not burkholderia multivorans. | while the epidemiology of burkholderia cepacia complex (bcc) bacteria in cystic fibrosis (cf) patients suggests that burkholderia multivorans is acquired from environmental sources, this species has rarely been isolated from soil and water samples. | 2016 | 26996269 |
| probing the size limit for nanomedicine penetration into burkholderia multivorans and pseudomonas aeruginosa biofilms. | encapsulation of antibiotics into nanoparticles is a potential strategy to eradicate biofilms. to allow further optimization of nanomedicines for biofilm eradication, the influence of the nanoparticle size on the penetration into dense biofilm clusters needs to be investigated. in the present study, the penetration of nanoparticles with diameters ranging from 40 to 550 nm into two biofilms, burkholderia multivorans lmg 18825 and pseudomonas aeruginosa lmg 27622, was evaluated using confocal micr ... | 2014 | 25125326 |
| growth on mannitol-rich media elicits a genome-wide transcriptional response in burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner. | in common with other members of the burkholderia cepacia complex (bcc), burkholderia multivorans is capable of producing exopolysaccharide (eps) when grown on certain mannitol-rich media. the significance of the resulting mucoid phenotype and the genome-wide response to mannitol has never been characterized despite its clinical relevance following the approval of a dried-powder preparation of mannitol as an inhaled osmolyte therapy for cystic fibrosis (cf) patients. in the present study we defin ... | 2014 | 24196427 |
| [detection of burkholderia cepacia complex strains in the university hospital olomouc]. | the aim was to assess the epidemiology of burkholderia cepacia complex strains isolated at the department of microbiology, faculty of medicine and dentistry, palacky university and university hospital olomouc, determine the most frequent strains and confirm or rule out potential clonal spread of the strains. | 2012 | 22484971 |
| a novel rhamno-mannan exopolysaccharide isolated from biofilms of burkholderia multivorans c1576. | burkholderia multivorans c1576 is a gram negative opportunistic pathogen causing serious lung infection in cystic fibrosis patients. considering that bacteria naturally form biofilms, and exopolysaccharides are recognized as important factors for biofilm architecture set-up, b. multivorans was grown both in biofilm and in non-biofilm mode on two different media in order to compare the exopolysaccharides biosynthesized in these different experimental conditions. the exopolysaccharides produced we ... | 2015 | 25974852 |
| impact of ozonation pre-treatment of oil sands process-affected water on the operational performance of a gac-fluidized bed biofilm reactor. | treatment of oil sands process-affected water (ospw) using biodegradation has the potential to be an environmentally sound approach for tailings water reclamation. this process is both economical and efficient, however, the recalcitrance of some ospw constituents, such as naphthenic acids (nas), require the pre-treatment of raw ospw to improve its biodegradability. this study evaluated the treatment of ospw using ozonation followed by fluidized bed biofilm reactor (fbbr) using granular activated ... | 2014 | 25104220 |
| raw meat contaminated with epidemic clones of burkholderia multivorans found in cystic fibrosis patients. | | 2015 | 25066362 |
| cell envelope phospholipid composition of burkholderia multivorans. | burkholderia multivorans causes opportunistic pulmonary infections in cystic fibrosis and immunocompromised patients. the purpose of the present study was to determine the nature of the phospholipids and their fatty acid constituents comprising the cell envelope membranes of strains isolated from three disparate sources. a conventional method for obtaining the readily extractable lipids fraction from bacteria was employed to obtain membrane lipids for thin-layer chromatographic and gas chromatog ... | 2014 | 24810292 |
| an enantioselective amidase from burkholderia multivorans for the stereoselective synthesis of esfenvalerate. | using racemic (r,s)-2-(4-chlorophenyl)-3-methylbutyramide, an intermediate for the chiral pyrethroid insecticide esfenvalerate, as a sole nitrogen source in a minimal medium, several strains with high enatioselectivity (≥98%) were isolated by enrichment techniques. one of the strains, lg 31-3, was identified as burkholderia multivorans, based on physiological and morphological tests by a standardized biolog station for carbon source utilization. a novel amidase was purified from b. mutivorans lg ... | 2014 | 24722372 |
| identification of burkholderia multivorans atcc 17616 genetic determinants for fitness in soil by using signature-tagged mutagenesis. | to identify bacterial genetic determinants for fitness in a soil environment, signature-tagged mutagenesis (stm) was applied to a soil bacterium, burkholderia multivorans atcc 17616. this strain was randomly mutagenized by each of 36 different signature-tagged plasposons, and 36 mutants with different tags were grouped as a set. a total of 192 sets consisting of 6912 independent mutants were each inoculated into soil and incubated. two-step stm screening based on quantitative real-time pcr of to ... | 2014 | 24530988 |
| radical sam enzyme quee defines a new minimal core fold and metal-dependent mechanism. | 7-carboxy-7-deazaguanine synthase (quee) catalyzes a key s-adenosyl-l-methionine (adomet)- and mg(2+)-dependent radical-mediated ring contraction step, which is common to the biosynthetic pathways of all deazapurine-containing compounds. quee is a member of the adomet radical superfamily, which employs the 5'-deoxyadenosyl radical from reductive cleavage of adomet to initiate chemistry. to provide a mechanistic rationale for this elaborate transformation, we present the crystal structure of a qu ... | 2014 | 24362703 |
| epidemiology of burkholderia multivorans strains obtained from non-cystic fibrosis patients isolated in large hospitals across the czech republic. | | 2014 | 24309418 |
| survey of bartonella spp. in u.s. bed bugs detects burkholderia multivorans but not bartonella. | bed bugs (cimex lectularius l.) have resurged in the united states and globally. bed bugs are hematophagous ectoparasites of humans and other animals, including domestic pets, chickens, and bats, and their blood feeding habits contribute to their potential as disease vectors. several species of bartonella are re-emergent bacterial pathogens that also affect humans, domestic pets, bats and a number of other wildlife species. because reports of both bed bugs and bartonella have been increasing in ... | 2013 | 24040015 |
| surfactant aided biodegradation of napls by burkholderia multivorans: comparison between triton x-100 and rhamnolipid jbr-515. | both chemical surfactants and biosurfactants have been effectively used for the degradation of petroleum hydrocarbons. chemical surfactants are known to enhance biodegradation effectively while activity of biosurfactants is also comparable and they have the additional advantage of being biodegradable. however, the mode of action of chemical surfactants and biosurfactants may vary. this work was conducted to determine the mode of action of triton x-100 and rhamnolipid jbr-515 by exploring the fac ... | 2013 | 23104033 |
| transport of nanoparticles in cystic fibrosis sputum and bacterial biofilms by single-particle tracking microscopy. | the aim of this study was to evaluate the effect of the surface functionalization of model nanoparticles on their mobility in bacterial biofilms and cystic fibrosis sputum. | 2013 | 23035662 |
| suppression of pleiotropic phenotypes of a burkholderia multivorans fur mutant by oxyr mutation. | fur (ferric uptake regulator) is an iron-responsive transcriptional regulator in many bacterial species, and the fur mutant of burkholderia multivorans atcc 17616 exhibits pleiotropic phenotypes, such as an inability to efficiently use several carbon sources, as well as high sensitivity to hydrogen peroxide (h(2)o(2)), paraquat (a superoxide-producing compound) and nitric oxide (no). to gain more insight into the pleiotropic role of the fur protein of atcc 17616, spontaneous suppressor mutants o ... | 2012 | 22361941 |
| pivotal role of anthranilate dioxygenase genes in the adaptation of burkholderia multivorans atcc 17616 in soil. | in our recent screen for soil-induced genes, the expression of anda operon (andacadabaa) for anthranilate catabolism in burkholderia multivorans atcc 17616 was found to increase dramatically in a soil sample (nishiyama et al., environ microbiol 12: 2539, 2010). the operon was preceded by andr encoding a putative transcriptional regulator for the anda operon. in this study, the anda promoter was induced by tryptophan and anthranilate in an andr-dependent manner. the anda promoter in a deletion mu ... | 2012 | 22360670 |
| an evaluation of different steam disinfection protocols for cystic fibrosis nebulizers. | contamination is a key element in cystic fibrosis. for this reason, nebulizer hygiene is an important, but complex and time-consuming task for cystic fibrosis patients. the aim of this study was to compare different steam disinfection and drying protocols. | 2016 | 26233897 |
| phosphate solubilization and gene expression of phosphate-solubilizing bacterium burkholderia multivorans ws-fj9 under different levels of soluble phosphate. | phosphate-solubilizing bacteria (psb) have the ability to dissolve insoluble phosphate and enhance soil fertility. however, the growth and mineral phosphate solubilization of psb could be affected by exogenous soluble phosphate and the mechanism has not been fully understood. in the present study, the growth and mineral phosphate-solubilizing characteristics of psb strain burkholderia multivorans ws-fj9 were investigated at six levels of exogenous soluble phosphate (0, 0.5, 1, 5, 10 and 20 mm). ... | 2017 | 28138122 |
| examining changes in bacterial abundance in complex communities using next-generation sequencing is enhanced with quantitative pcr. | changes in the composition of microbial communities are often examined using high-throughput sequencing (hts). here we analyzed bar-coded illumina sequencing data from a previous work describing the microbial community found in the sputum of a cystic fibrosis patient by itself or in combination with qpcr to measure the absolute abundance of pseudomonas aeruginosa and burkholderia multivorans. through this comparison we were able to determine the computational analysis accuracy of sequencing data ... | 2016 | 27160743 |