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sequence diversity of ns(m) movement protein of tospoviruses.in order to determine the diversity of the movement protein (ns(m)) among tospoviruses, the nsm genes of five distinct tospovirus species occurring in brazil (tomato chlorotic spot virus, groundnut ring spot virus, chrysanthemum stem necrosis virus, zucchini lethal chlorosis virus and iris yellow spot virus) were cloned, sequenced and compared with ns(m) sequences of other available tospoviruses. the 'd-motif', a conserved region present in the majority of '30k superfamily' virus movement protei ...200111556705
the glycoprotein gene of chrysanthemum stem necrosis virus and zucchini lethal chlorosis virus and molecular relationship with other tospoviruses.two tospoviruses, chrysanthemum stem necrosis virus (csnv) and zucchini lethal chlorosis virus (zlcv), cause economical losses in several ornamental and vegetable crops in brazil. the nucleocapsid gene and movement protein sequences had already been reported for both viruses, though the glycoprotein precursor gene sequence was not available. in this study, cdna fragments (ca. 4 kb) of the m rna 3' portion of csnv (isolate chry-1) and zlcv (isolate 1038), including the complete glycoprotein precu ...200717570049
increase of tospoviral diversity in brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini.abstract during a survey conducted in several different regions of brazil, two unique tospoviruses were isolated and characterized, one from chrysanthemum and the other from zucchini. the chrysanthemum virus displayed a broad host range, whereas the virus from zucchini was restricted mainly to the family cucurbitaceae. double-antibody sandwich-enzyme-linked immunosorbent assay and western immunoblot analyses demonstrated that both viruses were serologically distinct from all reported tospovirus ...199918944712
sequence determination and analysis of the nss genes of two tospoviruses.the tospoviruses groundnut ringspot virus (grsv) and zucchini lethal chlorosis virus (zlcv) cause severe losses in many crops, especially in solanaceous and cucurbit species. in this study, the non-structural nss gene and the 5'utrs of these two biologically distinct tospoviruses were cloned and sequenced. the nss sequence of grsv and zlcv were both 1,404 nucleotides long. pairwise comparison showed that the nss amino acid sequence of grsv shared 69.6% identity with that of zlcv and 75.9% identi ...201122187101
the complete genome of the tospovirus zucchini lethal chlorosis virus.zucchini lethal chlorosis virus (zlcv) causes significant losses in the production of cucurbits in brazil. this virus belongs to the genus tospovirus (family bunyaviridae) and seems to be exclusively transmitted by frankliniella zucchini (thysanoptera). tospoviruses have a tripartite and single-stranded rna genome classified as s (small), m (medium) and l (large) rnas. although zlcv was identified as a member of the genus tospovirus in 1999, its complete genome had not been sequenced until now.201627388209
development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to tomato spotted wilt virus.tospoviruses, the plant-infecting genus in the family bunyaviridae, are thrips borne and cause severe agricultural losses worldwide. based on the serological relationships of the structural nucleocapsid protein (np), the current tospoviruses are divided into six serogroups. the use of np-antisera is convenient for virus detection, but it is insufficient to identify virus species grouped in a serogroup due to the serological cross-reaction. alternatively, virus species can be identified by the n ...201728081705
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