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freeze-fracture and cytochemical studies on the in vitro cyst form of reptilian blastocystis pythoni.after cultured cysts are osmotically shocked by treating with distilled water, there is an exponential increase in the cyst form of blastocystis pythoni; this was demonstrated by an immunofluorescence antibody assay against the culture organisms. in 11-day-old cultures of b. pythoni, 68.8% of the organisms (= 2.2 x 10(8) cysts/ml) were in the cyst form. examination of thin sections of cysts revealed many similarities to the cyst forms of blastocystis obtained from fecal samples in previous inves ...200312674482
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