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preliminary pharmacological studies of pecarin, a new preparation from ribes nigrum fruits. 19863739735
comparative effects of total flavonoids extracted from ribes nigrum leaves, rutin and isoquercitrin on biosynthesis and release of prostaglandins in the ex vivo rabbit heart.experiments were carried out on isolated rabbit hearts: pg biosynthesis was induced by administration into the coronary circulation of a single dose of arachidonic acid (100 micrograms) and the pgs synthesized and released in the heart effluent were detected by bioassays. total flavonoids (tf) extracted from ribes nigrum leaves and their 2 major components, rutin and isoquercitrin had neither spasmodic nor relaxing activity on rat stomach strip. they were not capable of inducing any biosynthesis ...19863460101
anti-inflammatory evaluation of a hydroalcoholic extract of black currant leaves (ribes nigrum).a hydroalcoholic extract of black currant (ribes nigrum) leaves was tested on carrageenan-induced rat paw oedema. pharmacological activity was compared with indomethacin and niflumic acid using acute and chronic (21 or 28 days) oral treatment. black currant extract and lyophilisate revealed significant anti-inflammatory activity comparable to that seen with the reference substances, but without their ulcerogenic potential, even at high doses during chronic treatment.19892615431
[pharmacological properties of anthocyanosides from ribes nigrum in rats]. 19744849382
[flavonoids extracted from ribes nigrum l. and alchemilla vulgaris l.: 1. in vitro inhibitory activities on elastase, trypsin and chymotrypsin. 2. angioprotective activities compared in vivo].in vitro experiments were conducted on the inhibitory properties of extracts from ribes nigrum l. and alchemilla vulgaris l. (fractions a1 + a2, a1, a2) on activity of the proteolytic enzymes elastase, trypsin and alpha-chymotrypsin. extracts from ribes nigrum l. and alchemilla vulgaris l. (fraction a1) inhibited 50% of the activity of porcine pancreas elastase at concentrations of 0.56 mg/ml and 0.16 mg/ml, respectively, against a synthetic substrate. inhibition was less effective on activity o ...19863635653
characterization of gamma-linolenic acid in ribes seed.the total lipid content of fruit seeds of the ribes family ranges by weight from 18.3% in goose-berries (ribes uva crispa) to 30.5% in black currants (ribes nigrum). isolation procedures and analytical methods (gas chromatography, mass spectrometry, high performance thin layer chromatography and stereospecific analysis) demonstrate that the oils from ribes seeds contain up to 19% by weight of gamma-linolenic acid (gamma-la, c18:3, n-6) in black currant oil. this last ribes species thus constitut ...19846098796
lipase-mediated hydrolysis of blackcurrant oil.four commercially available lipases, both free and immobilized, were tested for their ability to catalyze hydrolysis of blackcurrant (ribes nigrum) oil using two different approaches. the lipase from mucor miehei was studied free and immobilized in two different ways. the former series of enzymic reactions were performed in tap water at 40 degrees c, but the latter series of enzymic processes were carried out in mixtures of isooctane and phosphate buffer (in a typical 2/1 ratio of the components ...200010978776
differential inhibition of eukaryote protein kinases by condensed tannins.condensed tannins, isolated from a variety of plant sources, were characterized according to the constituent flavans, being based on procyanidin and/or prodelphinidin and having a cis or trans stereochemistry at positions 2 and 3. all the tannin preparations are potent inhibitors of rat liver cyclic amp-dependent protein kinase catalytic subunit (cak) with ic50 values (concentrations for 50% inhibition) ranging from 0.009 to 0.2 microm. the tannin preparations are very good inhibitors of rat bra ...19968862029
enzyme-assisted extraction of antioxidative phenols from black currant juice press residues (ribes nigrum).enzymatic release of phenolic compounds from pomace remaining from black currant (ribes nigrum) juice production was examined. treatment with each of the commercial pectinolytic enzyme preparations grindamyl pectinase, macer8 fj, macer8 r, and pectinex be, as well as treatment with novozym 89 protease, significantly increased plant cell wall breakdown of the pomace. each of the tested enzyme preparations except grindamyl pectinase also significantly enhanced the amount of phenols extracted from ...200111453748
activity of anthocyanins from fruit extract of ribes nigrum l. against influenza a and b viruses.earlier, we have detected antiviral activity in an extract from ribes nigrum l. fruits ("kurokarin", name of the one species of black currant in japanese) against influenza a and b viruses, and herpes simplex virus 1 (knox et al., food processing 33, 21-23, 1998). in the present study, the antiviral activity of constituents of a kurokarin extract and the mechanism of its antiviral action were examined. kurokarin extracts were separated to fractions a to d by column chromatography. the major cons ...200111885927
effects of prodelphinidins isolated from ribes nigrum on chondrocyte metabolism and cox activity.articular diseases, such as osteoarthritis, is the clinical expression of the loss of cartilage function. cox inhibitors are widely used in the treatment of such pathologies for their beneficial effects on inflammation but often produce a negative activity on cartilage synthesis. in this study, we determined the effect of different prodelphinidins, the major compounds isolated from ribes nigrum leaves, on the proteoglycans (pgs), type ii collagen (coll. ii) and prostaglandin e(2) (pge(2)) produc ...200212070756
anti-influenza virus activity of crude extract of ribes nigrum l.this experiment was designed to detect the antiviral activities of crude fruit extracts of wild ribes nigrum l. (kurokarin extract) against influenza virus types a and b. kurokarin extract was prepared as follows: fruits of ribes nigrum l. were heated at 50 degrees c in a heating tank, and then ground under anaerobic conditions. the extracts were centrifuged, and the supernatant fluid was filtered and sterilized by infrared rays. the crude extract was diluted with eagle's minimum essential mediu ...200312601672
effects of dietary anthocyanins on tocopherols and lipids in rats.the effects of dietary cyanidin-3-o-glucoside (c3g) and concentrates from blackcurrant [ribes nigrum] (bc) and elderberry [sambucus nigra] (ec) on plasma and tissue concentrations of alpha- (alpha-t) and gamma-tocopherol (gamma-t) and cholesterol, as well as the fatty acid composition of the liver lipids were investigated in growing, male rats of the sprague-dawley strain. animals were fed semisynthetic diets supplemented with 2 g/kg c3g, bc, or ec for 4 weeks. dietary anthocyanins did not affec ...200212452636
antioxidant status, lipoprotein profile and liver lipids in rats fed on high-cholesterol diet containing currant oil rich in n-3 and n-6 polyunsaturated fatty acids.plant-based n-3 polyunsaturated fatty acids (pufa) possess a prospective antiatherogenic potential. currant oil from ribes nigrum l. is one of the few plant oils containing pufan-3 (15.3 mol%) in addition to pufan-6 (60.5 mol%). this study was aimed at comparing the effects of currant oil with those of lard fat, rich in saturated (43.8 mol%) and monounsaturated (47.0 mol%) fatty acids, on antioxidant parameters, the lipoprotein profile and liver lipids in rats fed on 1 % (w/w) cholesterol diets ...200312678660
mixed infection of black currant (ribes nigrum l.) plants with blackcurrant reversion associated virus and rhabdovirus-like particles with symptoms of black currant reversion disease.black currant plants of cvs. black smith and karlstejnský dlouhohrozen showing symptoms of severe russian (r) form of black currant reversion disease (bcrd) were found in 1999-2000 in the czech republic. five selected plants of both cultivars originating from two distant loci were tested by polymerase chain reaction (pcr) for presence of the blackcurrant reversion associated virus (brav), the causal agent of bcrd. in all plants, virus-specific 215 nt cdna fragments proving the presence of brav w ...200212693863
delphinidin-3-rutinoside relaxes the bovine ciliary smooth muscle through activation of etb receptor and no/cgmp pathway.delphinidin-3-rutinoside (d3r) is the major anthocyanin component in blackcurrant (ribes nigrum l.) fruits. we investigated the relaxation mechanism of d3r in bovine ciliary smooth muscle (cm). d3r at a concentration of 10(-5) m produced a sustained and progressive relaxation during the contraction induced by endothelin (et)-1 in the bovine cm specimens. after the pre-treatment with d3r, the anthocyanin exerted an inhibitory effect on the et-1-induced contraction with a concomitant increase in c ...200515721614
anti-herpesvirus activity of an extract of ribes nigrum l.an extract of ribes nigrum l., known as blackcurrant in europe and kurokarin(r) in japan, has been used as an ingredient in a variety of foods and folk medicine. in this study, the anti-herpesvirus activity of this extract was examined in vitro. the extract inhibited herpes simplex virus type 1 attachment on the cell membrane completely at a 100-fold dilution, as well as the plaque formation of herpes simplex virus types 1 and 2, and varicella-zoster virus by 50% at a 400-fold dilution or lower ...200312820226
inhibitory effects of proanthocyanidins from ribes nigrum leaves on carrageenin acute inflammatory reactions induced in rats.the anti-inflammatory effects of proanthocyanidins (pacs), isolated from blackcurrant (ribes nigrum l.) leaves, were analysed using carrageenin-induced paw oedema and carrageenin-induced pleurisy in rats.200415498105
high molecular weight polysaccharides from black currant seeds inhibit adhesion of helicobacter pylori to human gastric mucosa.several crude and purified polysaccharides from black currant seeds (ribes nigrum l.) have been isolated, analysed and examined on their effects against helicobacter pylori in in situ adhesion studies on sections of human gastric mucosa. after pre-treatment of helicobacter pylori with 0.01 to 0.1 % solutions of the isolated raw polysaccharide (rps), the epithelial binding of the bacteria was considerably reduced in a concentration-dependent manner, as compared with a non-treated control suspensi ...200415254855
proanthocyanidins, from ribes nigrum leaves, reduce endothelial adhesion molecules icam-1 and vcam-1.the effects of proanthocyanidins (pacs), isolated from blackcurrant (ribes nigrum l.) leaves, on neutrophil accumulation during inflammatory processes were investigated in vivo and in vitro.200516091140
characterization of a cdna encoding metallothionein 3 from cotton (gossypium hirsutum l.).a cdna encoding metallothionein (mt) was isolated from a library constructed with poly a(+) rna purified from 48 h etiolated cotton (gossypium hirsutum l.) cotyledons. this cdna encodes a deduced protein with 63 residues and a molecular weight of 6.3 kda. the protein has 10 cysteines of which 4 are within the cxxcxcxxxxxc amino-terminus motif and six are within the cxcxxxcxcxxcxc carboxyl-terminus motif characteristic of the type iii mt (mt3). the cotton mt3 protein sequence is 76.2, 69.8, 66.7, ...200516147860
immunostimulatory effects of a polysaccharide-rich substance with antitumor activity isolated from black currant (ribes nigrum l.).the fruit juice of black currant was found to contain a polysaccharide-rich substance, which was designated cassis polysaccharide (caps), with macrophage-stimulating activity. especially, its interleukin (il)-1beta-inducing activity was remarkably high, compared with other fruit juice preparations. caps was found to consist of rhamnose, mannose, arabinose, galactose, xylose, and glucose in a molar ratio of 11.3:0.9:54.1:29.8:2.0:1.9. caps turned out to be partitioned into a soluble component (ca ...200516306683
berry fruits: compositional elements, biochemical activities, and the impact of their intake on human health, performance, and disease.an overwhelming body of research has now firmly established that the dietary intake of berry fruits has a positive and profound impact on human health, performance, and disease. berry fruits, which are commercially cultivated and commonly consumed in fresh and processed forms in north america, include blackberry ( rubus spp.), black raspberry ( rubus occidentalis), blueberry ( vaccinium corymbosum), cranberry (i.e., the american cranberry, vaccinium macrocarpon, distinct from the european cranbe ...200818211023
cutin composition of five finnish berries.the raw cutin (i.e., extractive-free isolated cuticular membrane) fraction from finnish berries, sea buckthorn (hippophaë rhamnoides), black currant (ribes nigrum), cranberry (vaccinium oxycoccos), lingonberry (vaccinium vitis-idaea), and bilberry (vaccinium myrtillus), was depolymerized by naome-catalyzed methanolysis. the composition of cutin monomers was determined by gc-(ei)ms analysis either as methyl esters or as tmsi esters, with oh groups derivatized to tmsi ethers. there was a notable d ...200616417304
absorption of black currant anthocyanins by monolayers of human intestinal epithelial caco-2 cells mounted in ussing type chambers.anthocyanins (acns) have been reported to have multiple biological properties imparting benefits to human health. their role in human nutrition, however, needs to be related to biokinetic data, such as bioavailability. the purpose of the present study was to focus on the potential absorption of black currant ( ribes nigrum l.) acns. caco-2 monolayers were used as an in vitro model of the absorptive intestinal epithelium. for absorption studies, caco-2 cells grown on permeable filters were mounte ...200818540609
plant phenolics affect oxidation of tryptophan.the effect of berry phenolics such as anthocyanins, ellagitannins, and proanthocyanidins from raspberry (rubus idaeus), black currant (ribes nigrum), and cranberry (vaccinium oxycoccus) and byproducts of deoiling processes rich in phenolics such as rapeseed (brassica rapa l.), camelina (camelina sativa), and soy (glycine max l.) as well as scots pine bark (pinus sylvestris) was investigated in an h2o2-oxidized tryptophan (trp) solution. the oxidation of trp was analyzed with high-performance liq ...200818646765
yeast as a model system for identification of metabolic targets of a 'glucosamine complex' used as a therapeutic agent of osteoarthritis.this manuscript describes the effect of a glucosamine complex and its different constituents on the metabolism of yeast cells. indeed, the yeast model biosystem offers important advantages in the understanding of basic cellular and molecular processes. for example, the possibility to differentiate aerobic and anaerobic metabolism allows the measurement of glycolysis and mitochondria importance in the control of energetic metabolism and stress-responsive. yeast growth and division can be controll ...200818662850
effects of a new nutraceutical ingredient on allergen-induced sulphidoleukotrienes production and cd63 expression in allergic subjects.allergic diseases represent conditions affecting millions of individuals across the world. the objective of this study was to investigate the potential anti-allergic effects of a new nutraceutical ingredient, pantescal (bionap, italy), contained in different food supplements. pantescal is a mixture of plant extracts, such as capparis spinosa, olea europaea, panax ginseng and ribes nigrum. the study was a randomized, double-blind, placebo controlled design. 60 patients allergic to common aeroalle ...200818817894
the development of a pcr-based marker linked to resistance to the blackcurrant gall mite (cecidophyopsis ribis acari: eriophyidae).gall mite (cecidophyopsis ribis) is the most serious pest of blackcurrant (ribes nigrum l.), causing the damaging condition known as 'big bud' and also transmitting blackcurrant reversion virus (brv) within and between plantations. the identification of resistant germplasm is at present a time-consuming and expensive process, dependent on field infestation plots. resistance based on gene ce introgressed from gooseberry has been used in uk breeding programmes for blackcurrant. using a bulked segr ...200918813905
a high molecular arabinogalactan from ribes nigrum l.: influence on cell physiology of human skin fibroblasts and keratinocytes and internalization into cells via endosomal transport.an arabinogalactan protein (f2) was isolated in 1.5% yield from the seeds of ribes nigrum l. (grossulariaceae) by aqueous extraction and a one-step anion exchange chromatography on deae-sephacel with 24% galactose, 43% arabinose, and 20% xylose as main carbohydrate residues. methylation analysis revealed the presence of a 1,3-/1,3,6-galactose backbone, side chains from arabinose in different linkages, and terminal xylose residues. the polysaccharide which turned out to be an arabinogalactan prot ...200919368904
chemical and biological studies of ribes nigrum l. buds essential oil.ribes nigrum buds are used in medicine for the diuretic and antiseptic properties of their volatile compounds. we present in this paper comparative data concerning the chemical composition of ribes nigrum buds essential oils obtained from three blackcurrant varieties. essential oils were isolated by steam distillation and were analyzed by gas chromatography coupled with mass spectrometry. the ribes nigrum essential oils (extracted from all three varieties) exhibited similar and large antibacteri ...200819706967
production and characterization of distilled alcoholic beverages obtained by solid-state fermentation of black mulberry (morus nigra l.) and black currant (ribes nigrum l.).the present study was conducted to appraise the potential of black mulberry and black currant to be used as fermentation substrates for producing alcoholic beverages obtained by distillation of the fruits previously fermented with sacchromyces cerevisiae ifi83. in the two distillates obtained, the volatile compounds that can pose health hazards are within the limits of acceptability fixed by the european council (regulation 110/2008) for fruit spirits. however, the amount of volatile substances ...201020102198
identification and functional analysis of the genes encoding delta6-desaturase from ribes nigrum.gamma-linolenic acid (gamma-linolenic acid, gla; c18:3 delta(6, 9, 12)) belongs to the omega-6 family and exists primarily in several plant oils, such as evening primrose oil, blackcurrant oil, and borage oil. delta(6)-desaturase is a key enzyme involved in the synthesis of gla. there have been no previous reports on the genes encoding delta(6)-desaturase in blackcurrant (ribes nigrum l.). in this research, five nearly identical copies of delta(6)-desaturase gene-like sequences, named rnd8a, rnd ...201020231328
anthocyanin-rich black currant extract suppresses the growth of human hepatocellular carcinoma cells.dietary antioxidants, such as anthocyanins, are helpful in the prevention and control of various diseases by counteracting the imbalance of oxidative and antioxidative factors in the living systems. black currant (ribes nigrum l., grossulariaceae) is known to contain high amounts of anthocyanins (250 mg/100 g fresh fruit). black currant fruits have been used in asian and european traditional medicine for the treatment of a variety of diseases. black currant extract has recently been found to be ...201021121259
black currant reversion virus, a mite-transmitted nepovirus.summary taxonomy: black currant reversion virus (brv) is the first identified mite-transmitted member of the genus nepovirus (family comoviridae). a few systematic studies have been performed to compare virus isolates from different geographical locations. physical properties: purified preparations contain two closely sedimenting centrifugal components (b and m for rna1 and rna2, respectively) at varying ratios, and occasionally a t component (for satellite rna). the brv capsids have a diameter ...200420565606
anthocyanin-rich black currant (ribes nigrum l.) extract affords chemoprevention against diethylnitrosamine-induced hepatocellular carcinogenesis in rats.anthocyanins are known to possess potent anticarcinogenic properties against several cancers thus demonstrating potential for cancer prevention. black currant (ribes nigrum l., grossulariaceae) fruits have a high anthocyanin content. this "superfruit" is known to possess various pharmacological effects including alleviation of chronic oxidative stress and inflammation. in contrast to a large volume of literature on the health benefits of black currant, limited evidence on antitumor effects of bl ...201121216582
Thin-layer chromatography with stationary phase gradient as a method for separation of water-soluble vitamins.The group of hydrophilic vitamins play an important role in human health, and their lack or excess produces specific diseases. Therefore, the analysis of these compounds is indispensable for monitoring their content in pharmaceuticals and food in order to prevent some human diseases. TLC was successfully applied in the analysis of hydrophilic vitamins, but the most difficult problem in the simultaneous analysis of all these compounds is to find an optimum stationary phase-mobile phase system due ...201122209545
ascorbic acid, phenolic acid, flavonoid, and carotenoid profiles of selected extracts from ribes nigrum.small fruits such as berries have low energy contents, but high contents of vitamins, micronutrients, and dietary fibers and constitute a good source of natural antioxidant compounds that are important constituents of the human diet. this study identified a large number of compounds in an extract of black currant showing high antioxidant activity and compared their profile in various parts of the plants (leaves, buds, and berries). if it was known that berries contained very high levels of natur ...201121417457
a new strategy for identification of currant (ribes nigrum l.) cultivars using rapd markers.we developed a new approach using rapd fingerprints to distinguish 37 currant cultivars from northeastern china based on optimization of rapd by choosing 11 nucleotide primers and strict screening pcr annealing temperature. we found that the manual cultivar identification diagram (mcid) approach clearly developed fingerprints from 8 different primers that were useful for cultivar identification; a cultivar identification diagram (cid) was readily constructed. this cid allows efficient currant cu ...201323913385
antiviral activity of ladania067, an extract from wild black currant leaves against influenza a virus in vitro and in vivo.influenza, a respiratory disease caused by influenza viruses, still represents a major threat to humans and several animal species. besides vaccination, only two classes of drugs are available for antiviral treatment against this pathogen. thus, there is a strong need for new effective antivirals against influenza viruses. here, we tested ladania067, an extract from the leaves of the wild black currant (ribes nigrum folium) for potential antiviral activity against influenza a virus in vitro and ...201424795704
black currant (ribes nigrum l.) and bilberry (vaccinium myrtillus l.) fruit juices inhibit adhesion of asaia spp.the aim of the study was to evaluate the activity of high-polyphenolic black currant (ribes nigrum l.) and bilberry (vaccinium myrtillus l.) juices against bacterial strains asaia lannensis and asaia bogorensis isolated as spoilage of commercial soft drinks. the composition of fruit juices was evaluated using chromatographic techniques hplc and lc-ms. the adhesion to glass, polystyrene, and polyethylene terephthalate in two different culture media was evaluated by luminometry and the plate count ...201627747228
anti-viral and anti-bacterial activities of an extract of blackcurrants (ribes nigrum l.).the inhibitory effects of an extract of the blackcurrant (ribes nigrum l.) against pathogens associated with oral, nasopharyngeal and upper respiratory infectious diseases; namely respiratory syncytial virus (rsv), influenza virus a and b (ifv-a and ifv-b), adenovirus (adv), herpes simplex virus type 1, haemophilus influenzae type b, streptococcus pneumoniae and streptococcus mutans, were investigated. less than 1% concentration of extract of blackcurrant inhibited replication of rsv, ifv-a and ...201222985050
antiadhesive properties of arabinogalactan protein from ribes nigrum seeds against bacterial adhesion of helicobacter pylori.fruit extracts from black currants (ribes nigrum l.) are traditionally used for treatment of gastritis based on seed polysaccharides that inhibit the adhesion of helicobacter pylori to stomach cells. for detailed investigations an arabinogalactan protein (f2) was isolated from seeds and characterized concerning molecular weight, carbohydrate, amino acid composition, linkage, configuration and reaction with β-glucosyl yariv. functional testing of f2 was performed by semiquantitative in situ adhes ...201424662083
anti-influenza virus activity of two extracts of the blackcurrant (ribes nigrum l.) from new zealand and poland.we investigated the inhibitory effect of extracts of blackcurrant (ribes nigrum l.) from new zealand and poland on 4 strains of influenza virus (ifv) by the inhibition of virus adsorption; pandemic flu from 2009-2010 (ifv-ah1pdm), hong kong flu (ifv-ah3), oseltamivir phosphate-resistant russian flu (ifv-ah1tamr) and influenza virus type b (ifv-b). the inhibitory effect of the extracts of blackcurrant or blueberry on the infectivity of the virion were evaluated by the inhibition of virus adsorpti ...201323842512
complete genome sequence and analysis of blackcurrant leaf chlorosis associated virus, a new member of the genus idaeovirus.blackcurrant leaf chlorosis associated virus (bclcav) was isolated from symptomatic blackcurrants (ribes nigrum cv. baldwin). the virus has a genome organization similar to that of raspberry bushy dwarf virus (rbdv), the type member of the genus idaeovirus. the rna-1of this virus encodes the replicase complex (orf1, mr 197 kda), while rna-2 encodes a putative movement protein (orf2a, mr 38.8 kda) and the putative coat protein (orf2b, mr 30 kda). a concatenated form of bclcav rna-2 was detected b ...201728190194
protective effects of raw and cooked blackcurrant extract on dna damage induced by hydrogen peroxide in human lymphoblastoid cells.blackcurrant (ribes nigrum l.) is a classical fruit that has long been used to make juice, liqueur and sometimes medicines in europe. the beneficial effects of blackcurrant, which are inhibition of lipopolysaccharide-stimulated inflammatory, anticarcinogenesis and other health effects, have been reported.201424392718
impact of pectin type on the storage stability of black currant (ribes nigrum l.) anthocyanins in pectic model solutions.the effects of different pectins on the stabilisation of black currant anthocyanins in viscous model solutions at ph 3.0 were investigated. for this purpose, low esterified amidated (am), low (lm) and high (hm) methoxylated citrus and apple pectins and a sugar beet pectin were added to a purified anthocyanin extract (acn-e) and to an extract containing anthocyanins and non-anthocyanin phenolics (pp-e). model systems were stored at 20±0.5°c in the dark. anthocyanin contents were monitored by hplc ...201323561223
application of plant extracts for the prevention of dental erosion: an in situ/in vitro study.antiadherent and antibacterial effects of certain plant extracts have been proven to be beneficial in preventive dentistry. in the present in situ/in vitro crossover study, the impact of plant extracts rich in polyphenols on the erosion-protective properties of the in situ pellicle was evaluated.201526228871
nonvolatile chemical cues affect host-plant ranking by gravid polygonia c-album females.in a multiple-choice test, the preference of egg-laying polygonia c-album (comma butterfly) females was studied for oviposition on plants bearing surrogate leaves treated with crude methanol extracts obtained from leaves of seven host-plant species: humulus lupulus, urtica dioica, ulmus glabra, salix caprea, ribes nigrum, corylus avellana, and betula pubescens. the ranking order of surrogate leaves treated with host-plant extracts corresponded well to that reported on natural foliage, except r. ...201222486046
evaluation of polyphenolic profile and nutritional value of non-traditional fruit species in the czech republic--a comparative study.dry matter, organic acids, ascorbic acid, minerals (nitrogen, phosphorus, potassium, calcium, magnesium, sodium) and polyphenolic profile of a number of non-traditional fruit species and their genotypes, namely blue honeysuckle (lonicera spp.), saskatoon berry (amelanchier alnifolia), black mulberry (morus nigra), tomentosa cherry (prunus tomentosa thunb.) and jostaberry (ribes nigrum x grossularia uva-crispa) were investigated. the results showed that lonicera genotypes displayed high levels of ...201222842642
some physiological responses to d,l abscisin (dormin).the responses to synthetic d,l abscisin have been studied in a variety of tests. when fed in aqueous solution continuously to leaves of seedlings growing under long day conditions, d,l abscisin caused the cessation of extension growth and the formation of typical resting-buds in betula pubescens, acer pseudoplatanus and ribes nigrum. abscisin also inhibited the growth of non-dormant buds of potato when applied to the whole tubers, but was much less effective when applied to isolated tuber plugs. ...196724554370
comparison of iso-eluotropic mobile phases at different temperatures for the separation of triacylglycerols in non-aqueous reversed phase liquid chromatography.triacylglycerols (tags) are a large class of neutral lipids that naturally occur in both plant and animal oils and fats. their analyses in non-aqueous reversed phase liquid chromatography (narp) require a mixture of weak solvent (mostly acetonitrile) and strong solvent. in the present work, we have established eluotropic solvent strength scale of several binary mobile phases on c18 bonded silica at different temperatures (acetonitrile/methylene chloride, acetonitrile/acetone, acetonitrile/ethyl ...201525855317
triacylglycerol biosynthesis in developing ribes nigrum and ribes rubrum seeds from gene expression to oil composition.oils with sufficient contents of fatty acids, which can be metabolized into precursors of anti-inflammatory eicosanoids, have potential health effects. ribes sp. seed oil is rich in α-linolenic, γ-linolenic and stearidonic acids belonging to this fatty acid group. only a few previous studies exist on ribes sp. gene expression. we followed the seed oil biosynthesis of four ribes nigrum and two ribes rubrum cultivars at different developmental stages over 2 years in southern and northern finland w ...201626593580
optimisation of the azinobis-3-ethyl-benzothiazoline-6-sulphonic acid radical scavenging assay for physiological studies of total antioxidant activity in woody plant germplasm.a robust spectroscopic method for determining total antioxidant activity in aqueous extractions has been applied to tissues from diverse woody plant species, including seeds of coffea arabica and in vitro shoots from ribes nigrum, picea sitchensis and shorea leprosula. the assay involves scavenging of an abts [2,2'-azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid)] radical generated by the reaction of potassium persulphate with abts to produce an abts*(+) chromophore (lambda=734 nm). antioxida ...200616762559
inhibition of cytosolic phospholipase a2α (cpla2α) by medicinal plants in relation to their phenolic content.the cytosolic phospholipase a2α(cpla2α) is one of the potential targets for anti-inflammatory drugs, since this enzyme plays a key role in the inflammation processes seen in health disorders, like asthma, allergic reactions, arthritis and neuronal diseases. in this study, cpla2α inhibition by 43 methanol extracts from medicinal plants rich in polyphenols was determined. the eight most active extracts were derived from ribes nigrum (ic50 of 27.7 μg/ml), ononis spinosa (ic50 of 39.4 μg/ml), urtica ...201526287155
phenolic compounds in berries of black, red, green, and white currants (ribes sp.).multiple health benefits associated with phenolic compounds have raised the interest in the contents of these plant metabolites in foods. several phenolic compounds were quantified from berries of ribes nigrum (black and green currants) and ribes x pallidum (red and white currants), by using sequential extraction with ethyl acetate and methanol and an optimized reversed-phase hplc method with diode array detection. the highest contents of anthocyanins (3,011 mg/kg fresh weight, expressed as the ...200111813993
hplc method for measurement of human salivary α-amylase inhibition by aqueous plant extracts.control of hyperglycemia is an important treatment in metabolic disorders such as type ii diabetes and obesity. α-amylase, as the first enzyme of glucose release from dietary polysaccharides, is a potential target to identify new sources of novel anti-obesity and anti-diabetic drugs. in this work, different herbal extracts as α-amylase inhibitors were studied by measuring the rate of the cleavage of a maltooligomer substrate 2-chloro-4-nitrophenyl-β-d-maltoheptoside. measurement of chromophore c ...201728605974
a plant extract of ribes nigrum folium possesses anti-influenza virus activity in vitro and in vivo by preventing virus entry to host cells.infections with influenza a viruses (iav) are still amongst the major causes of highly contagious severe respiratory diseases not only bearing a devastating effect to human health, but also significantly impact the economy. besides vaccination that represents the best option to protect from iav infections, only two classes of anti-influenza drugs, inhibitors of the m2 ion channel and the neuraminidase, often causing resistant iav variants have been approved. that is why the need for effective an ...201323717460
complete genome sequence of a strain of actinidia virus x detected in ribes nigrum cv. baldwin showing unusual symptoms.a ribes-infecting strain of the potexvirus actinidia virus x (avx-rv3124) was isolated from black currant plants (ribes nigrum cv. baldwin, accession 3124-03d1) showing symptoms of leaf chlorosis and deformity. this is the first description of the complete genome sequence of an isolate of this virus and the first detection of a potexvirus in ribes. the genome of avx-rv3124 consists of 6,888 nucleotides (nt) excluding the poly(a) tail at the 3' terminus. when avx-rv3124 was compared to the availa ...201626586329
a gastrointestinally digested ribes nigrum l. fruit extract inhibits inflammatory response in a co-culture model of intestinal caco-2 cells and raw264.7 macrophages.blackcurrant fruits are a rich source of polyphenolic compounds with high antioxidant capacity and potent anti-inflammatory properties. in this study, blackcurrant extract digested in an artificial gastrointestinal tract and its intestinal permeable fraction were investigated for their ability to suppress inflammatory responses induced in a two-component cell culture system of intestinal epithelial cells and macrophages. the obtained results showed the capacity of the extract at a concentration ...027666411
application of sample disruption methods in the extraction of anthocyanins from solid or semi-solid vegetable samples.cassis, a commercial powder obtained from blackcurrant juice (ribes nigrum l.), was used to optimize the anthocyanin extraction by matrix solid-phase dispersion (mspd) and by sea sand disruption method (ssdm). the optimum eluent conditions were 5.0 ml of meoh/h2o (1:1, v/v) at ph 2. the extracts were analyzed by hplc with diode-array detection (dad) and the extraction efficiency was evaluated by statistical comparison (analysis of variance, anova-single factor) of the compounds peak areas. the e ...200616893546
characterization of phenolic profiles of northern european berries by capillary electrophoresis and determination of their antioxidant activity.berries are known to contain phenolic substances (i.e., flavonoids and phenolic acids), which comprise two large and heterogeneous groups of biologically active nonnutrients. this investigation evaluated the content and profile of the phenolic compounds present in six different berries found in northern europe. the latter included bilberry (vaccinium myrtillus), cowberry (vaccinium vitis-idaea), cranberry (vaccinium oxycoccus), strawberry (fragaria ananassa), black currant (ribes nigrum), and re ...200516076138
determination of anthocyanidins in berries and red wine by high-performance liquid chromatography.a high-performance liquid chromatographic (hplc) method for the determination of anthocyanidins from berries and red wine is described. delphinidin, cyanidin, petunidin, pelargonidin, peonidin, and malvidin contents of bilberry (vaccinium myrtillus), black currant (ribes nigrum), strawberry (fragaria ananassa cv. jonsok), and a cabernet sauvignon (vitis vinifera) red wine were determined. the aglycon forms of the anthocyanins present in the samples were revealed by acid hydrolysis. a reversed ph ...200111559107
polyphenolic extracts of cherry (prunus cerasus l.) and blackcurrant (ribes nigrum l.) leaves as natural preservatives in meat products.the objective of this study was to evaluate the possibility of using polyphenolic extracts from cherry and blackcurrant leaves as natural antimicrobial agents in meat products. the polyphenolic composition of the extracts was analyzed and their impact on the microbial quality, lipid oxidation, color, and sensory evaluation of pork sausages was studied. polyphenolic extracts were obtained from leaves collected in september. the total polyphenolic content in sour cherry leaf extract was 1.5 times ...201627375255
polysaccharide from black currant (ribes nigrum l.) stimulates dendritic cells through tlr4 signaling.black currant (ribes nigrum) has various beneficial properties for human health. in particular, polysaccharide from black currant was found to be an immunostimulating food ingredient and was reported to have antitumor activity in a mouse model. we named it cassis polysaccharide (caps). in a previous study, caps administration caused tumor necrosis factor-α (tnf-α) and interleukin-1β (il-1β) production in vitro and in vivo, but the immunological mechanism of caps was not demonstrated. in this stu ...201729038769
analysis and sensory evaluation of volatile constituents of fresh blackcurrant (ribes nigrum l.) fruits.volatile constituents of fresh blackcurrant (ribes nigrum l.) berries were isolated via vacuum-headspace extraction and analyzed by capillary gas chromatography-mass spectrometry. in agreement with previous studies with frozen fruits, short-chain esters and terpenes were major compound classes. however, rather high concentrations of c6-compounds (e.g., (e)-hex-2-enal, (z)-hex-3-enal) constituted a striking difference to data reported for frozen fruits. frozen storage of blackcurrant berries was ...201728992408
anthocyanin‑rich blackcurrant extract inhibits proliferation of the mcf10a healthy human breast epithelial cell line through induction of g0/g1 arrest and apoptosis.blackcurrants (ribes nigrum l., grossulariaceae) possess a high content of anthocyanin polyphenols, which have been demonstrated to exhibit beneficial effects on health due to their antioxidant and anticarcinogenic prope-rties. the present study investigated novel functions of anthocyanin‑rich blackcurrant extracts (bces) in a healthy mammary epithelial cell line, mcf10a. the percentages of viable cells were 85, 75, 53 and 31% following exposure to 50, 100, 200 and 400 µg/ml bce, respectively. t ...201728901377
postprandial glycaemic response to berry nectars containing inverted sucrose.sucrose is commonly used for sweetening berry products. during processing and storage of berry products containing added sucrose, sucrose is inverted to glucose and fructose. we have previously shown that postprandial glycaemic response induced by intact sucrose is attenuated when sucrose is consumed with berries rich in polyphenols. it is not known how inversion of sucrose affects glycaemic response. we investigated postprandial glycaemic and insulinaemic responses to blackcurrant (ribes nigrum ...201728620479
hypotensive, cardiodepressant, and vasorelaxant activities of black currant (ribes nigrum 'ben sarek') juice.the aim of this study was to evaluate the effects of black currant (ribes nigrum l. 'ben sarek') juice on the blood pressure and frequency of cardiac contractions, as well as vasomotor responses of rat aortic rings. arterial blood pressure was measured directly from the carotid artery in the anaesthetized rabbits. the aortic rings were pre-contracted with kcl (80 mmol·l-1), after which black currant juice was added. an intravenous injection of black currant juice (0.33-166.5 mg·kg-1) induced a s ...201627564244
ascorbate pool, sugars and organic acids in black currant (ribes nigrum l.) berries are strongly influenced by genotype and post-flowering temperature.marked effects of the climatic environment on fruit chemical composition have often been demonstrated in field experiments. however, complex covariations of several climatic factors in the natural environment complicate the interpretation of such experiments and the identification of the causal factors. this can be better achieved in a phytotron where the various climatic factors can be varied systematically. therefore, we grew four black currant cultivars of contrasting origin in a phytotron un ...201727328984
changing the content of phenolic compounds as the response of blackcurrant (ribes nigrum l.) leaves after blackcurrant leaf midge (dasineura tetensi rübs.) infestation.blackcurrant leaf midge (dasineura tetensi) is one of the most common pests of blackcurrant (ribes nigrum). the aim of this study was to investigate changes in the content of phenolic compounds in the leaves damaged by the larvae of this pest. additionally, susceptibility of different blackcurrant cultivars to the midge attack was investigated. qualitative and quantitative analyses of control and pest-infested blackcurrant leaves were performed using lc-pda-qtof/ms and uplc-pda-fl systems. a tot ...201627161581
black currant (ribes nigrum l.) extract induces apoptosis of mkn-45 and te-1 cells through mapk- and pi3k/akt-mediated mitochondrial pathways.black currant extract (bce) is rich in polyphenols and can induce apoptosis in various cancer cells, but the molecular mechanism by which bce induces cancer cell apoptosis has not been reported. the aim of this work was to elucidate the antitumor effect of bce and the signal transduction pathways involved. mtt test results revealed that the viability of mkn-45 and te-1 cells treated with bce gradually decreased in a concentration-dependent manner, with significant effects achieved after 12 h of ...201627007806
antioxidant capacities and anthocyanin characteristics of the black-red wild berries obtained in northeast china.various edible berries widely accessible in nature in northeast china are poorly exploited. the compositions and contents of anthocyanins in black (padus maackii, padus avium, lonicera caerulea, and ribes nigrum) and red (ribes rubrum, sambucus williamsii, rubus idaeus, and ribes procumbens) wild berries in northeast china were firstly characterized by hplc-dad/esi-ms(2). twenty-three anthocyanins were detected and identified. cyanidin glycosides were dominant in both berries. six anthocyanins w ...201626988488
biomolecules and natural medicine preparations: analysis of new sources of bioactive compounds from ribes and rubus spp. buds.it is well known that plants are important sources for the preparation of natural remedies as they contain many biologically active compounds. in particular, polyphenols, terpenic compounds, organic acids, and vitamins are the most widely occurring groups of phytochemicals. some endemic species may be used for the production of herbal preparations containing phytochemicals with significant bioactivity, as antioxidant activity and anti-inflammatory capacities, and health benefits. blackberry spro ...201626861353
influence of controlled postflowering temperature and daylength on individual phenolic compounds in four black currant cultivars.the effects of postflowering temperature and daylength on the concentration of individual phenolic compounds were studied in black currant (ribes nigrum l.) berries under controlled phytotron conditions. the four cultivars studied varied greatly in their concentrations of individual phenolic compounds and temperature stability for accumulation. the concentrations of a wide range of identified phenolic compounds were strongly influenced by temperature over the 12-24 °c range, often with opposite ...201626758764
accumulation of anthocyanins and flavonols in black currants (ribes nigrum l.) by pre-harvest methyl jasmonate treatments.chemical elicitation is one of the most effective methods currently used to enrich plant foods in bioactive compounds. methyl jasmonate (mj) has been described as a very useful elicitor of some plant compounds, polyphenols among them. the objective of this study was to determine the effects of pre-harvest mj application on the increase in the main flavonoids and the antioxidant properties of black currant (ribes nigrum) cultivars.201626694740
blackcurrant suppresses metabolic syndrome induced by high-fructose diet in rats.increased fructose ingestion has been linked to obesity, hyperglycemia, dyslipidemia, and hypertension associated with metabolic syndrome. blackcurrant (ribes nigrum; bc) is a horticultural crop in europe. to induce metabolic syndrome, sprague-dawley rats were fed 60% high-fructose diet. treatment with bc (100 or 300 mg/kg/day for 8 weeks) significantly suppressed increased liver weight, epididymal fat weight, c-reactive protein (crp), total bilirubin, leptin, and insulin in rats with induced me ...201526504474
optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity.pressurised water extraction (pwe) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (rsm). the optimal conditions for pwe were: time 51min, pressure 1.6mpa, and temperature 52°c. under these conditions, the experimental yield of ribes nigrum l. polysaccharides (rnlp) was 11.68±0.12%, which closely agreed with the predicted value (11.77%). after preliminary purification with d4006 macroporous resin, rnlp i was obtained and its chemical characterisa ...201626471604
flavonol glycosides in currant leaves and variation with growth season, growth location, and leaf position.flavonol glycosides (fg) were analyzed in the leaves of six currant cultivars (ribes spp.) with hplc-dad, hplc-ms/ms, and nmr. the average amounts of the 12 major, identified fg constituted 86-93% (9.6-14.1 mg/g dw) of the total of 27 fg found. quercetin and kaempferol were the major aglycones with trace amounts of myricetin. quercetin-3-o-(2,6-α-dirhamnopyranosyl-β-glucopyranoside), quercetin-3-o-(2-β-xylopyranosyl-6-α-rhamnopyranosyl-β-glucopyranoside), and kaempferol-3-o-(3,6-α-dirhamnopyrano ...201526448427
phytoestrogenic activity of blackcurrant (ribes nigrum) anthocyanins is mediated through estrogen receptor alpha.blackcurrants (ribes nigrum l., grossulariaceae) contain high amounts of anthocyanin polyphenols, which have antioxidant and anti-carcinogenic health benefits. this study analyzed the potential phytoestrogenic effects of blackcurrant extract (bce) in breast cancer (mcf-7) and human endometrial cancer (ishikawa) cell lines that over-express estrogen receptor alpha (erα), as well as in immature female rats.201526395027
biological and nutritional properties of blackcurrant berries (ribes nigrum l.) under conditions of shading nets.changes of environmental factors, created under the influence of various shading nets, could significantly affect the biological characteristics of plants grown in such conditions as well as biosynthesis of primary metabolites and ascorbic acid. five blackcurrant cultivars - 'ben sarek', 'ben nevis', 'ben lomond', 'ometa' and 'čačanska crna' - were cultivated in the shade of two green polyethylene nets and exposed to direct sunlight during two experimental seasons.201526216726
phenolic composition of "bud extracts" of ribes nigrum l., rosa canina l. and tilia tomentosa m.liquid preparations such as tinctures and "bud extracts" are typical products widely used in european countries but which have been poorly studied to date. our research was focused on obtaining a phytochemical characterization of secondary metabolites of black currant, dog rose and silver linden bud extracts and evaluating the changes of selected chemical markers over time. 16 different preparations have been analyzed by the use of hplc-dad-ms. several flavonols were identified in black currant ...201526135753
black currant anthocyanins attenuate weight gain and improve glucose metabolism in diet-induced obese mice with intact, but not disrupted, gut microbiome.black currant (ribes nigrum l.) is a rich source of anthocyanins; however, the relationship between their apparently limited bioavailability and significant protection against metabolic pathologies is poorly understood. this study examined the gastrointestinal distribution of black currant anthocyanins and their phenolic acid metabolites in lean and diet-induced obese mice with healthy and antibiotic-disrupted microbiomes. daily consumption of low- or high-fat diet supplemented with 1% black cur ...201526066489
cloning of an anthocyanidin synthase gene homolog from blackcurrant (ribes nigrum l.) and its expression at different fruit stages.anthocyanidin synthase (ans), a 2-oxoglutarate (2og) and fe(ii)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. in this study, a full-length, 1427-bp long cdna named rnans1, which is homologous to the anthocyanidin synthase gene, was cloned from blackcurrant using a homologous cloning strategy. rnans1 is highly homologous to other plant ans genes at both the nucleot ...201525867421
winter warming delays dormancy release, advances budburst, alters carbohydrate metabolism and reduces yield in a temperate shrub.global climate models predict an increase in the mean surface air temperature, with a disproportionate increase during winter. since temperature is a major driver of phenological events in temperate woody perennials, warming is likely to induce changes in a range of these events. we investigated the impact of slightly elevated temperatures (+0.76 °c in the air, +1.35 °c in the soil) during the non-growing season (october-april) on freezing tolerance, carbohydrate metabolism, dormancy release, sp ...201525802249
phenolic compounds in blackcurrant (ribes nigrum l.) leaves relative to leaf position and harvest date.blackcurrant leaves are an essential source of phenolic compounds and this study investigated their variation relative to leaf positions and harvest date. the phenolic content varied between harvest dates, although leaf position on the shoot and interactions also played an important role. the contents of quercetin-malonyl-glucoside, kaempferol-malonyl-glucoside isomer and kaempferol-malonyl-glucoside were higher than that of the other identified phenolic compounds, whereas epigallocatechin was t ...201525442534
extract from ribes nigrum leaves in vitro activates nitric oxide synthase (enos) and increases cd39 expression in human endothelial cells.the aim of the present study was to evaluate whether blackcurrant leaf extract (ble) modulates endothelium antithrombotic function, namely increases the expression/activity of adpase (cd39) and augments the production of nitric oxide in human umbilical vein endothelial cells (huvec). it was found that ble with proanthocyanidins (60 % of the total polyphenol content) increased the cd39-positive endothelial cell fraction (up to 10 % for 2.5 μg/ml, and up to 33 % for 15 μg/ml, p < 0.05 or less) in ...201425407137
essential oil from black currant buds as chemotaxonomy marker and antimicrobial agent.dormant buds are recognized as valuable side product of the blackcurrant cultivation. four blackcurrant varieties cultivated in serbia, i.e., ben sarek, ometa, ben lomond, and ben nevis, were evaluated for the content, chemical composition, and antimicrobial activity of their bud essential oils. the oil yields of buds harvested during two different growth periods ranged from 1.2-2.0%, and the variety ometa had the highest yield among the tested varieties. gc-fid and gc/ms analysis of the oils al ...201425146766
flavonol glycosides and other phenolic compounds in buds and leaves of different varieties of black currant (ribes nigrum l.) and changes during growing season.phenolic compounds in buds and leaves of three varieties of black currant in finland were identified by hplc-dad-esi-ms/ms. forty-three phenolic compounds of flavonol glycosides, proanthocyanidins and phenolic acids were found in variety "mikael" whereas only thirty-five in "mortti" and "jaloste n:o 15". glycosides of quercetin and kaempferol were the major phenolics. rutin, hyperoside, isoquercitrin, kaempferol-3-o-rutinoise, kaempferol-3-o-glucoside, quercetin-3-o-(6″-malonyl)-glucoside and a ...201424799225
detection of the polyphenolic components in ribes nigrum l.the blackcurrant (ribes nigrum l.) is a species of native currant which contains a lot of polyphenolic antioxidants which is used medicinally and has a fundamental role in the maintenance health. materials, methods and objective: ultraviolet-visible spectrophotometry and ultraviolet range high performance liquid chromatography (hplc) were used to characterize the polyphenolic content of common ribes nigrum collected in the western part of the banat region in romania.201424742033
biological activity of blackcurrant extracts (ribes nigrum l.) in relation to erythrocyte membranes.compounds contained in fruits and leaves of blackcurrant (ribes nigrum l.) are known as agents acting preventively and therapeutically on the organism. the hplc analysis showed they are rich in polyphenol anthocyanins in fruits and flavonoids in leaves, that have antioxidant activity and are beneficial for health. the aim of the research was to determine the effect of blackcurrant fruit and leaf extracts on the physical properties of the erythrocyte membranes and assess their antioxidant propert ...201424527456
chemical profile and antioxidative and antimicrobial activity of juices and extracts of 4 black currants varieties (ribes nigrum l.).the aim of this article was to determine the chemical composition (anthocyanin and ascorbic acid contents, total phenols, and tannins), and the antioxidative, and antimicrobial activities of the juices and methanol extracts of 4 varieties-tenah, triton, ben sarek, and ometa. the juices were made during 2008, 2009, and 2010 from the berries grown on the same location. statistically significant differences were found in all the tested parameters of each variety for every year. ometa variety exhibi ...201424506271
phenolics, antioxidative activity and characterization of anthocyanins in berries of blackcurrant interspecific hybrids.the interest in phenols and anthocyanins has increased due to their antioxidant properties and to their potential usage as dietary antioxidants in human nutrition. total phenols and anthocyanin content, composition and stability in berry extracts of blackcurrant interspecific hybrids, and antioxidative activity of extracts was evaluated. berries of interspecific hybrids accumulated 530 to 614 mg 100 g(-1) fw of total phenolic compounds, while 621 mg 100 g(-1) fw of phenolics was established in b ...201324432329
phenols and ascorbic acid in black currants (ribes nigrum l.): variation due to genotype, location, and year.black currant berries contain many biochemical compounds with proven or potential human health benefits. we studied the content of total and single polyphenols, ascorbic acid, soluble sugars, and titratable acidity for two advanced selections and three cultivars of black currant at two distant locations in sweden (south: 56°06'n; north: 65°21'n) over a 3 year period. regression analyses revealed the effect of genotype to be considerably larger than that of location and year. however, significant ...201324011264
analysis and sensory evaluation of jostaberry (ribes x nidigrolaria bauer) volatiles.volatiles of jostaberries (ribes x nidigrolaria bauer)--a hybrid of black currant (ribes nigrum l.) and gooseberry (ribes uva-crispa l.)--were isolated via vacuum headspace extraction and analyzed by capillary gas chromatographic methods for the first time. (e)-hex-2-enal, (e)-hex-2-en-1-ol, (z)-hex-3-enal, (z)-hex-3-en-1-ol, methyl butanoate, ethyl butanoate, 2-methylbut-3-en-2-ol, and 1,8-cineol turned out to be the most dominant volatiles. the variability of the volatile profile was shown by ...201323991667
antioxidant lignoids from leaves of ribes nigrum.phytochemical investigation of the leaves of ribes nigrum resulted in the isolation of fourteen compounds, including four 7,7'-epoxylignans, three tetrahydrofuran-type sesquilignans, and a spirocyclic dilignan. their structures were elucidated by extensive spectroscopic analyses and by chemical transformations. the isolated compounds were evaluated for their antioxidant activities using superoxide anion scavenging assay and dpph free radical scavenging assay. ribesin d and ribesin g showed the m ...201323958345
molecular characterization of a novel rhabdovirus infecting blackcurrant identified by high-throughput sequencing.a large contig with sequence similarities to several nucleorhabdoviruses was identified by high-throughput sequencing analysis from a black currant (ribes nigrum l.) cultivar. the complete genome sequence of this new nucleorhabdovirus is 14,432 nucleotides long. its genomic organization is very similar to those of unsegmented plant rhabdoviruses, containing six open reading frames in the order 3'-n-p-p3-m-g-l-5. the virus, which is provisionally named "black currant-associated rhabdovirus", is 4 ...201829368064
characterization, antioxidant and hypoglycemic activities of degraded polysaccharides from blackcurrant (ribes nigrum l.) fruits.in this study, the degradation of polysaccharides from blackcurrant (bcp) was investigated. two low-molecular-weight polysaccharides (dbcp-1, dbcp-2) were obtained using fe2+ with different concentrations of h2o2 solution. ir spectra showed dbcps had obvious characteristic peaks of polysaccharides. gc analysis confirmed dbcps were composed of the same monosaccharide units as bcp but with different molar ratios. nmr analysis indicated dbcps and bcp had similar glycosidic linkage patterns. the sur ...201829146337
metabolic fingerprinting of dormant and active flower primordia of ribes nigrum using high-resolution magic angle spinning nmr.global warming may modify the timing of dormancy release and spring growth of buds of temperate fruit crops. environmental regulation of the activity-dormancy cycle in perennial plants remains poorly understood at the metabolic level. especially, the fine-scale metabolic dynamics in the meristematic zone within buds has received little attention. in this work we performed metabolic profiling of intact floral primordia of ribes nigrum isolated from buds differing in dormancy status using high-res ...201729083175
botanicals in ribes nigrum bud-preparations: an analytical fingerprinting to evaluate the bioactive contribution to total phytocomplex.context. ribes nigrum l. (grossulariaceae) is among the most commonly used herbal medicines and it is popularized for its alleged tonic effect and curative and restorative properties. the current practice of identifying herbal extracts is by measuring the concentration of the main botanicals. their concentrations are used to characterize the herbal preparations and fingerprinting is recommended by the main pharmacopeias as a potential and reliable strategy for the quality control of complex mixt ...201323844599
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