| geminivirus-mediated gene silencing from cotton leaf crumple virus is enhanced by low temperature in cotton. | a silencing vector for cotton (gossypium hirsutum) was developed from the geminivirus cotton leaf crumple virus (clcrv). the clcrv coat protein gene was replaced by up to 500 bp of dna homologous to one of two endogenous genes, the magnesium chelatase subunit i gene (chli) or the phytoene desaturase gene (pds). cotyledons of cotton cultivar 'deltapine 5415' bombarded with the modified viral vectors manifested chlorosis due to silencing of either chli or pds in approximately 70% of inoculated pla ... | 2008 | 18621976 |
| cotton leaf crumple virus is a distinct western hemisphere begomovirus species with complex evolutionary relationships indicative of recombination and reassortment. | abstract the bipartite dna genome of cotton leaf crumple virus (clcrv), a whitefly-transmitted begomovirus from the sonoran desert, was cloned and completely sequenced. the cloned clcrv genome was infectious when biolistically delivered to cotton or bean seedlings and progeny virus was whitefly-transmissible. koch's postulates were completed by the reproduction of characteristic leaf crumple symptoms in cotton seedlings infected with cloned clcrv dna, thereby verifying the etiology of leaf crump ... | 2004 | 18943795 |
| method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system. | we previously developed a virus-induced gene silencing (vigs) vector for cotton from the bipartite geminiviruscotton leaf crumple virus (clcrv). the original clcrv vigs vector was designed for biolistic delivery by a gene gun. this prerequisite limited the use of the system to labs with access to biolistic equipment. here we describe the adaptation of this system for delivery by agrobacterium (agrobacterium tumefaciens). we also describe the construction of two low-cost particle inflow guns. | 2012 | 22853641 |
| a versatile system for functional analysis of genes and micrornas in cotton. | cotton is an important economic crop worldwide. due to its long growth cycle, large genome size and recalcitrance to stable transformation, traditional methods for the analysis of gene function in this crop are difficult and labour intensive. here, we report a cotton leaf crumple virus (clcrv)-based vector and its application in gene function analysis through virus-induced gene silencing (vigs) and overexpression of micrornas (mirnas), small tandem target mimic (sttm) and artificial mirna (amirn ... | 2014 | 24521483 |
| virus-induced gene silencing of fiber-related genes in cotton. | virus-induced gene silencing (vigs) is a useful method for transient downregulation of gene expression in crop plants. the geminivirus cotton leaf crumple virus (clcrv) has been modified to serve as a vigs vector for persistent gene silencing in cotton. here the use of green fluorescent protein (gfp) is described as a marker for identifying silenced tissues in reproductive tissues, a procedure that requires the use of transgenic plants. suggestions are given for isolating and cloning combination ... | 2015 | 25740368 |
| overexpression of ft in cotton affects architecture but not floral organogenesis. | flowering marks the change from indeterminate to determinate plant growth, and this developmental transition involves the activity of the arabidopsis flowering locus t (ft) gene product and its orthologs. we demonstrated that when ft is ectopically expressed from a viral vector in cotton, a process referred to as virus induced flowering (vif), it uncouples flowering from photoperiodic regulation and promotes determinate growth in aerial organs. the accelerated switch to determinate growth affect ... | 2013 | 23333977 |
| geminivirus-mediated delivery of florigen promotes determinate growth in aerial organs and uncouples flowering from photoperiod in cotton. | plant architecture and the timing and distribution of reproductive structures are fundamental agronomic traits shaped by patterns of determinate and indeterminate growth. florigen, encoded by flowering locus t (ft) in arabidopsis and single flower truss (sft) in tomato, acts as a general growth hormone, advancing determinate growth. domestication of upland cotton (gossypium hirsutum) converted it from a lanky photoperiodic perennial to a highly inbred, compact day-neutral plant that is managed a ... | 2012 | 22615805 |
| molecular characterization and phylogenetic relationships of desmodium leaf distortion virus (deldv): a new begomovirus infecting desmodium glabrum in yucatan, mexico. | the complete dna-a component sequence of desmodium leaf distortion virus (deldv, begomovirus) isolated in yucatan was determined to be 2569 nucleotides (nt) in length, and it was most closely related to cotton leaf crumple virus-california (clcrv-[cal]), at 76%. the complete dna-b component sequence was 2514 nt in length, and shared its highest nucleotide identity (60%) with potato yellow mosaic trinidad virus (pymtv). phylogenetic analyses group the deldv dna-a component in the slcv clade, wher ... | 2009 | 19757008 |