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cold stress and nitrogen deficiency affected protein expression of psychrotrophic dyadobacter psychrophilus b2 and pseudomonas jessenii mp1.nitrogen (n) deficiency and low temperature conditions are the prominent facet of western himalayan agro-ecosystems. a slight change in the environment alters the protein expression of the microorganisms. therefore, proteomes of the two psychrotrophs dyadobacter psychrophilus b2 and pseudomonas jessenii mp1 were analyzed using two dimensional electrophoresis and maldi-tof-ms, to determine the physiological response of altitudinally different but indigenous microorganisms in response to cold stre ...201728352263
biosurfactant production by pseudomonas strains isolated from floral nectar.to screen and identify biosurfactant-producing pseudomonas strains isolated from floral nectar; to characterize the produced biosurfactants; and to investigate the effect of different carbon sources on biosurfactant production.201525801599
pseudomonas helmanticensis sp. nov., isolated from forest soil.a bacterial strain, oha11(t), was isolated during the course of a study of phosphate-solubilizing bacteria occurring in a forest soil from salamanca, spain. the 16s rrna gene sequence of strain oha11(t) shared 99.1% similarity with respect to pseudomonas baetica a390(t), and 98.9% similarity with the type strains of pseudomonas jessenii, pseudomonas moorei, pseudomonas umsongensis, pseudomonas mohnii and pseudomonas koreensis. the analysis of housekeeping genes rpob, rpod and gyrb confirmed its ...201424744015
description of pseudomonas jessenii subsp. pseudoputida subsp. nov., amended description of pseudomonas jessenii and description of pseudomonas jessenii subsp. jessenii subsp. nov.fluorescent pseudomonas putida ccm 3656 (atcc 11250) was analysed according to the methods of polyphasic approach which were based on sequence analyses involving the rpob and rrs genes, manual ribotyping using endonuclease hindiii, dna base composition determination and dna-dna hybridization. the results obtained by these genotyping methods showed that the strain ccm 3656 is distant from p. putida taxon, which was supported with phenotype characterization represented by whole-cell protein profil ...201323636777
two draft genome sequences of pseudomonas jessenii strains isolated from a copper contaminated site in denmark.pseudomonas jessenii c2 and pseudomonas jessenii h16 were isolated from low-cu and high-cu industrially contaminated soil, respectively. p. jessenii h16 displayed significant resistance to copper when compared to p. jessenii c2. here we describe genome sequences and interesting features of these two strains. the genome of p. jessenii c2 comprised 6,420,113 bp, with 5814 protein-coding genes and 67 rna genes. p. jessenii h16 comprised 6,807,788 bp, with 5995 protein-coding genes and 70 rna genes. ...201627833718
pseudomonas baetica sp. nov., a novel fish pathogen isolated from wedge sole, dicologoglossa cuneata (moreau).five gram negative bacterial isolates recovered from an outbreak occurred in march 2006 in huelva, spain, affecting adult diseased cultured wedge sole (dicologoglossa cuneata), were phenotypically and genotypically characterized in order to clarify its taxonomic position. on the basis of 16s rrna gene sequence analysis isolates were included in the genus pseudomonas, within the p. fluorescens-related species group, being their closest relatives the p. jessenii and p. koreensis subgroups. the hig ...201121642488
off-odor compounds produced in cork by isolated bacteria and fungi: a gas chromatography-mass spectrometry and gas chromatography-olfactometry study.the risk of development of specific olfactory profiles in cork was evaluated after inoculation of cork granules and agglomerated and natural cork stoppers with isolated bacteria and fungi. the highest incidence of off-odor development was found in assays when fungi were inoculated. cork granules with musty-earthy, musty-earthy-tca, and vegetative deviations were inspected by gas chromatography-olfactometry (gc-o) and gas chromatography-mass spectrometry (gc-ms). sixteen odor zones were clearly r ...200919639991
soil type-dependent effects of a potential biocontrol inoculant on indigenous bacterial communities in the rhizosphere of field-grown lettuce.bacterial biocontrol strains used as an alternative to chemical fungicides may influence bacterial communities in the rhizosphere and effects might differ depending on the soil type. here we present baseline data on the effects of pseudomonas jessenii ru47 on the bacterial community composition in the rhizosphere of lettuce grown in diluvial sand, alluvial loam and loess loam at the same field site. 16s rrna gene fragments amplified from total community dna were analyzed by denaturing gradient g ...201425244497
soil type dependent rhizosphere competence and biocontrol of two bacterial inoculant strains and their effects on the rhizosphere microbial community of field-grown lettuce.rhizosphere competence of bacterial inoculants is assumed to be important for successful biocontrol. knowledge of factors influencing rhizosphere competence under field conditions is largely lacking. the present study is aimed to unravel the effects of soil types on the rhizosphere competence and biocontrol activity of the two inoculant strains pseudomonas jessenii ru47 and serratia plymuthica 3re4-18 in field-grown lettuce in soils inoculated with rhizoctonia solani ag1-ib or not. two independe ...201425099168
isolation and characterization of phosphate solubilizing bacteria from western indian himalayan soils.previous studies confirmed the existence of diversified microbial flora in the rhizosphere of himalayan red kidney bean (rkb) (phaseolus vulgaris l.). therefore, fifteen different temperate and subtropical regions of western indian himalaya (wih) were explored for the isolation of rkb rhizosphere-associated phosphorus (p) solubilizing bacteria. on the basis of qpcr analysis, three soils, i.e., munsyari, kandakhal and nainital soils were selected for the isolation of p solubilizers. among 133 iso ...201728555431
characterization of the cultivable microbiota of sprouts and their potential for application as protective cultures.the microbiota of ten seeds and ready-to-eat sprouts produced thereof was characterized by bacteriological culture and denaturing gradient gel electrophoresis (dgge) of amplified dna fragments of the 16s rrna gene. the predominant bacterial biota of hydroponically grown sprouts mainly consisted of enterobacteria, pseudomonades and lactic acid bacteria (lab). for adzuki, alfalfa, mung bean, radish, sesame and wheat, the ratio of these bacterial groups changed strongly in the course of germination ...200717512686
effect of heterologous expression of phag [(r)-3-hydroxyacyl-acp-coa transferase] on polyhydroxyalkanoate accumulation from the aromatic hydrocarbon phenylacetic acid in pseudomonas species.five pseudomonas strains capable of growth with the aromatic carboxylic acid phenylacetic acid were investigated with a view to improving pha accumulation. the overexpression of (r)-3-hydroxyacyl-acp-coa transferase (phag) from pseudomonas putida ca-3 increased pha accumulation in only one of the five strains tested, namely pseudomonas jessenii c8. recombinant p. jessenii c8 harbouring the phag gene showed a 4.1-fold increase (9.6-39% cell dry weight) in pha accumulation when grown on phenylacet ...200717241246
low-substrate regulated microaerophilic behavior as a stress response of aquatic and soil bacteria.low-substrate regulated microaerophilic behavior (lsrmb) was observed in 10-54% of the bacteria isolated from several fresh-water lakes or ponds, subsurface soils, activated sludge, and antarctic dry valley soils. five pseudomonas and two bacillus type species showed lsrmb. a subsurface pseudomonas jessenii strain was used as a model to show the metabolic interaction between substrate and oxygen concentrations, cell band movement, and the appearance of unique stress lipids and proteins. when the ...200010856370
influence of metal resistant-plant growth-promoting bacteria on the growth of ricinus communis in soil contaminated with heavy metals.the metal resistant-plant growth-promoting bacterial (pgpb) strains psm6 and pjm15 isolated from a serpentine soil were characterized as pseudomonas sp. and pseudomonas jessenii, respectively, on the basis of their morphological, physiological, biochemical characteristics and 16s rdna sequences. assessment of plant growth-promoting parameters revealed the intrinsic ability of the strains for the utilization of 1-aminocyclopropane-1-carboxylic acid as the sole n source, solubilization of insolubl ...200818164365
efficacy of lactoferricin b in controlling ready-to-eat vegetable spoilage caused by pseudomonas spp.the microbial content of plant tissues has been reported to cause the spoilage of ca. 30% of chlorine-disinfected fresh vegetables during cold storage. the aim of this work was to evaluate the efficacy of antimicrobial peptides in controlling microbial vegetable spoilage under cold storage conditions. a total of 48 bacterial isolates were collected from ready-to-eat (rte) vegetables and identified as belonging to acinetobacter calcoaceticus, aeromonas media, pseudomonas cichorii, pseudomonas flu ...201526453993
siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads.a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ...200212039729
pseudomonas koreensis sp. nov., pseudomonas umsongensis sp. nov. and pseudomonas jinjuensis sp. nov., novel species from farm soils in korea.among pseudomonas strains isolated from korean agricultural soils, four strains (ps 9-14 group: ps 1-2, ps 1-10, ps 5-5 and ps 9-14t) from the suwon, goesan and samchok regions, three strains (ps 3-10 group: ps 2-22, ps 3-1 and ps 3-10t) from umsong region and four strains (pss 26 group: pss 14, pss 25, pss 26t and pss 27) from jinju region were identified as three independent groups on the basis of 16s rdna sequence analysis. while, on the basis of 16s rdna sequence analysis, ps 9-14t and ps 3- ...200312656147
in vitro antagonists of rhizoctonia solani tested on lettuce: rhizosphere competence, biocontrol efficiency and rhizosphere microbial community response.the rhizosphere competence of 15 in vitro antagonists of rhizoctonia solani was determined 4 weeks after sowing inoculated lettuce seeds into nonsterile soil. based on the colonization ability determined by selective plating, eight strains were selected for growth chamber experiments to determine their efficacy in controlling bottom rot caused by r. solani on lettuce. although in the first experiment all antagonists colonized the rhizosphere of lettuce with cfu counts above 2 x 10(6) g(-1) of ro ...200919486156
stress tolerance and genetic variability of phosphate-solubilizing fluorescent pseudomonas from the cold deserts of the trans-himalayas.nineteen efficient phosphate-solubilizing fluorescent pseudomonas from the cold deserts of the trans-himalayas were screened for stress tolerance against temperature, alkalinity, salinity, calcium salts, and desiccation. phylogenetic analysis based on 16s rrna gene sequencing placed these bacteria under three groups with fourteen strains in group i including pseudomonas trivialis and p. poae, two strains in group ii together with pseudomonas kilonensis and p. corrugata, and three strains in grou ...200919319589
antagonistic effects of pseudomonas jessenii against pythium aphanidermatum: morphological, ultrastructural and cytochemical aspects.pseudomonas jessenii isolate ec-s101, an antagonistic rhizobacterium, induces morphological abnormalities such as topical swelling and excessive lateral branching in phytopathogenic peronosporomycetes pythium aphanidermatum hyphae as a result of radial growth inhibition in a dual culture assay. rhodamine-phalloidin staining revealed that these abnormalities were associated with disorganization of actin cytoskeleton. both the morphological forms of actin, filaments and plaques, were affected prog ...200818383229
taxonomic study of bacteria isolated from natural mineral waters: proposal of pseudomonas jessenii sp. nov. and pseudomonas mandelii sp. nov.the taxonomic position of 23 strains isolated from mineral waters and previously grouped in the authentic pseudomonads on the basis of a phenotypic analysis (cluster ix, subclusters xiiia and xiiic of verhille, s., elomari, m., coroler, l., izard, d., leclerc, h. (syst. appl. microbiol, 20, 137-149, 1997) has been genotypically further studied in the present work. on the basis of hybridization results, these strains were gathered into two new genomic groups for which we propose the names of pseu ...199910188278
taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as pseudomonas putida.one hundred and forty-four fluorescent pseudomonad strains isolated from various environments (soil, water, plant rhizosphere, hospital) and received as pseudomonas putida (83 strains), p. putida biovar a (49 strains), p. putida biovar b (10 strains) and p. putida biovar c (2 strains), were analysed by the pyoverdine-isoelectrofocusing and pyoverdine-mediated iron uptake methods of siderotyping. both methods demonstrated a great diversity among these strains, which could be subdivided into 35 si ...200717978216
isolation and structure elucidation of peronosporomycetes hyphal branching-inducing factors produced by pseudomonas jessenii ec-s101.pseudomonas jessenii ec-s101 produced hyphal branching-inducing and mitosis-accelerating factors active towards peronosporomycetes, aphanomyces cochlioides hyphae. in searching for the active substances, etoac-solubles extracted from ec-s101-cultured solid medium were fractionated under the guidance of a paper disc assay using an a. cochlioides mycelium. two active substances were subsequently isolated and the structure was elucidated by spectroscopic analysis to be (+)-4,5-didehydroacaterin (1) ...200717587701
pseudomonas moraviensis sp. nov. and pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of pseudomonas asplenii.two strains of gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. on the basis of 16s rrna gene sequence analysis, the two strains were found to belong to the genus pseudomonas, within the gammaproteobacteria. strain 1b4t shared the highest sequence similarity with pseudomonas koreensis dsm 16610t (99.5%) and pseudomonas jessenii ccm 4840t (99.3%), and strain 2b2t with pseudomonas asplenii dsm 17133t (98.9%), pseu ...200617082407
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