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potential probiotics from indian major carp, cirrhinus mrigala. characterization, pathogen inhibitory activity, partial characterization of bacteriocin and production of exoenzymes.the study explored antagonistic activity of the cellular components of potential probiotic bacteria from mrigal (cirrhinus mrigala) against fish pathogens with a basic insight of the chemical nature of the antagonistic compound. totally 208 autochthonous gut bacteria were isolated, of which 22 strains revealed antagonism towards ≥2 of the six common fish pathogens. zones of inhibition (halo diameter) were presented as score and the four most promising strains were selected as putative probiotics ...201627663374
[isolation and identification of a bacterial strain khg2 with high resistance to mercury and cloning and expression of its mera gene].the aim of our study was to isolate and identify mera gene from a bacterial strain with high resistance to mercury.200920222449
complete genome sequence and characterization of the n-acylhomoserine lactone-degrading gene of the potato leaf-associated solibacillus silvestris.n-acylhomoserine lactones (ahls) are used as quorum-sensing signal molecules by many gram-negative bacteria. we have reported that solibacillus silvestris, which was isolated from the potato leaf, has ahl-degrading activity. to identify the ahl-degrading gene, whole genome sequencing of s. silvestris stlb046 was performed by using pyrosequencing technology. as the result of the blast search, one predicted orf (ahls) showed slight similarity to aiia-like ahl lactonase from bacillus cereus group. ...201122019407
re-examination of the taxonomic position of bacillus silvestris rheims et al. 1999 and proposal to transfer it to solibacillus gen. nov. as solibacillus silvestris comb. nov.following the transfer of three of the six species enclosed in the original definition of rrna group 2 of bacillus to the genus sporosarcina and two to lysinibacillus, other species of this group, some of which were added later, still await taxonomic revision. in a recent publication, a set of 'core' characteristics was proposed for species to be included in the genus bacillus (kämpfer et al., 2006). except for bacillus silvestris, however, several or none of these properties are available for m ...200919406792
amylolytic microorganism from s+úo paulo zoo composting: isolation, identification, and amylase production.composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. this transformation is the result of the microorganism action, converting complex carbon sources into energy. enzymes that are exported by the microorganisms to the surrounding environment mediate this process. the aiming of the present work is to prospect the compost produced by the organic composting unit (ocu) of the funda+º+úo parque zool+¦gic ...201121845217
characterization of acetanilide herbicides degrading bacteria isolated from tea garden soil.three different green manures were added to the tea garden soils separately and incubated for 40 days. after, incubation, acetanilide herbicides alachlor and metolachlor were spiked into the soils, separately, followed by the isolation of bacteria in each soil at designed intervals. several bacterial strains were isolated from the soils and identified as bacillus silvestris, b. niacini, b. pseudomycoides, b. cereus, b. thuringiensis, b. simplex, b. megaterium, and two other bacillus sp. (met1 an ...200817661128
characterization of a novel thermostable n-acylhomoserine lactonase from the thermophilic bacterium thermaerobacter marianensis.thermaerobacter marianensis is an extremely thermophilic bacterium, which was isolated from the mariana trench, with an optimal growth temperature of approximately 75 °c. n-acylhomoserine lactone (ahl) is a quorum-sensing signal molecule used by many gram-negative bacteria. here, we report the identification of an ahl-degrading gene homolog (designated aiit) in the genome of t. marianensis jcm 10246. aiit has 59.7%, 21.2%, and 11.2% identity to ahls from solibacillus silvestris, aiia from bacill ...201525529553
bacillus cecembensis sp. nov., isolated from the pindari glacier of the indian himalayas.strain pn5(t) is a gram-positive, aerobic, motile, rod-shaped, peritrichously flagellated bacterium that was isolated from the pindari glacier using nutrient agar medium. cells of pn5(t) are catalase-positive and oxidase-negative and contain lysine, glutamic acid and alanine in the peptidoglycan (peptidoglycan type a4alpha). further, the cells are characterized by the presence of iso-c(15 : 0) and iso-c(16 : 1) as the predominant fatty acids and mk-7 as the isoprenoid quinone. based on the above ...200818842851
phylogeny of bacillus sphaericus-like organisms.the mesophilic round-spored bacteria embrace four species, namely bacillus sphaericus, bacillus fusiformis, bacillus silvestris and bacillus pasteurii. although not displayed by all strains, mosquito pathogenicity is a noteworthy characteristic of b. sphaericus sensu lato. phylogenetic analysis based on 16s rdna sequences from 58 strains identified as b. sphaericus was used to examine the genetic heterogeneity of the taxon. results from sequence analysis were compared with whole-cell fatty acid ...200011034479
antineoplastic agents. 570. isolation and structure elucidation of bacillistatins 1 and 2 from a marine bacillus silvestris.two new cyclodepsipeptides designated bacillistatins 1 (1) and 2 (2) have been isolated from cultures of a sample of bacillus silvestris that was obtained from a pacific ocean (southern chile) crab. each 12-unit cyclodepsipeptide strongly inhibited growth of a human cancer cell line panel, with gi(50)'s of 10(-4)-10(-5) microg/ml, and each compound was active against antibiotic-resistant streptococcus pneumoniae. the structures were elucidated by a combination of x-ray diffraction and mass and 2 ...200919226154
bacillus silvestris sp. nov., a new member of the genus bacillus that contains lysine in its cell wall.a gram-positive, aerobic, rod-shaped, peritrichously flagellated, round-endospore-forming bacterium was isolated from a forest soil near braunschweig, lower saxony, germany, and designated strain hr3-23t (t = type strain). morphologically, strain hr3-23t shows the characteristics of a member of the genus bacillus. the spore position is terminal in a swollen sporangium. comparative analysis of the 16s rdna sequence shows strain hr3-23t to be most closely related to caryophanon tenue (95.8% 16s rr ...199910319505
bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the mars odyssey spacecraft.a round-spore-forming bacillus species that produces an exosporium was isolated from the surface of the mars odyssey spacecraft. this novel species has been characterized on the basis of phenotypic traits, 16s rdna sequence analysis and dna-dna hybridization. according to the results of these analyses, this strain belongs to the genus bacillus and is a gram-positive, aerobic, rod-shaped, endospore-forming eubacterium. ultrathin sections of the spores showed the presence of an exosporium, spore c ...200414742480
microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the indian intertidal zone.isolates were obtained from intertidal zone site samples from all five western and one eastern coastal states of india and were screened. these ecophysiological groups of aerobic, mesophilic, heterotrophic, sporulating, and bioemulsifier-producing bacteria were from planococcaceae and bacillaceae. this is the first report of bioemulsifier production by sporosarcina spp., lysinibacillus spp., b. thuringiensis, and b. flexus. in this group, solibacillus silvestris am1 was found to produce the high ...201627068903
bioemulsifier (be-am1) produced by solibacillus silvestris am1 is a functional amyloid that modulates bacterial cell-surface properties.a novel estuarine bacterial strain, solibacillus silvestris am1, produces an extracellular, thermostable and fibrous, glycoprotein bioemulsifier (be-am1). the amyloid nature of the bioemulsifier (be-am1) was confirmed by biophysical techniques (congo red based polarization microscopy, thioflavins based fluorescent microscopy, fibrous arrangement in transmission electron microscopy and secondary structure measurement by ftir and cd spectrum analysis). cell-bound be-am1 production by s. silvestris ...201627669827
bacillus massiliensis sp. nov., isolated from cerebrospinal fluid.an unidentified gram-negative-staining, aerobic, rod-shaped, spore-forming bacterium was isolated from a sample of cerebrospinal fluid. based on comparative analysis of 16s rrna gene sequences and phenotypic characteristics, the novel isolate was included in the bacillus sphaericus-like group. the isolate was closely related to bacillus odysseyi and bacillus silvestris, with 96.2 and 94.4 % 16s rrna gene sequence similarity, respectively. the major fatty acid was iso-c(15 : 0) (48 %). the name b ...200616825616
bioflocculant production from solibacillus silvestris w01 and its application in cost-effective harvest of marine microalga nannochloropsis oceanica by flocculation.microalgae are widely studied for biofuel production, however, current technologies to harvest microalgae for this purpose are not well developed. in this work, a bacterial strain w01 was isolated from activated sludge and identified as solibacillus silvestris. bioflocculant in the culture broth of w01 showed 90% flocculating efficiency on marine microalga nannochloropsis oceanica, and no metal ion was required for the flocculation process. chemical analysis of the purified bioflocculant indicat ...201323218529
the diversity of the fecal bacterial community and its relationship with the concentration of volatile fatty acids in the feces during subacute rumen acidosis in dairy cows.sub-acute ruminal acidosis (sara) is a well-recognized digestive disorder found in particular in well-managed dairy herds. sara can result in increased flow of fermentable substrates to the hindgut, which can increase the production of volatile fatty acids, alter the structure of the microbial community, and have a negative effect on animal health and productivity. however, little is known about changes in the structure of the microbial community and its relationship with fatty acids during sara ...201223217205
reclassification of bacillus isronensisshivaji et al. 2009 as solibacillus isronensis comb. nov. and emended description of genus solibacillus krishnamurthi et al. 2009.an investigation into the taxonomic position of bacillus isronensis mtcc 7902t revealed that the strain shares a common phylogenetic lineage with solibacillus silvestris mtcc 10789t. it displays considerable overlap in phenotypic properties with the genus solibacillus, including endospore shape and position, oxidase and catalase activities, presence of iso-c15 : 0, c16 : 1ω7c alcohol and iso-c17 : 1ω7c as major cellular fatty acids, a4α-type cell-wall peptidoglycan, polar lipids and menaquinone ...201626907585
antineoplastic agents. 600. from the south pacific ocean to the silstatins.the recent advances in the development of antibody and other drug conjugates for targeted cancer treatment have further increased the need for powerful cancer cell growth inhibitors. toward that objective we have extended our earlier discovery of the remarkable anticancer bacillistatins 1 and 2 from bacillus silvestris to sar and other structural modifications such as availability of a free hydroxy group for antibody-drug conjugate (adc) and other prodrug linkage. that direction has resulted in ...201525688575
solibacillus kalamii sp. nov., isolated from a high-efficiency particulate arrestance filter system used in the international space station.a gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated issfr-015t, was isolated from a high-efficiency particulate arrestance filter in the international space station and was characterized by polyphasic taxonomy. a comparative analysis of the 16s rrna gene sequence (1494 bp) of strain issfr-015t showed highest similarity to solibacillus isronensis b3w22t (98.9 %), followed by solibacillus silvestris hr3-23t (98.6 %) and bacillus cecembensis pn5t (96.7 %). dna ...201728475026
diversity of bacteria and archaea from a landfill in chandigarh, india as revealed by culture-dependent and culture-independent molecular approaches.the bacterial community structure of a municipal landfill in chandigarh, india was analysed by culture-dependent as well as culture-independent molecular approaches, and archaeal structure by the latter method. samples were collected in two phases from the surface and a depth of 0.91 m in june, 2004 and from 0.91 m, 1.52 m and 1.68 m in may, 2005. after serial dilutions, samples were plated onto tryptic soy agar (tsa), plate count agar (pca), tryptic soy broth agar (tsba) and tsba100 (tsba dilut ...201223274043
iron-dependent growth of and siderophore production by two heterotrophic bacteria isolated from brackish water of the southern baltic sea.iron is indispensable to the growth and metabolism of all marine organisms, including bacteria. in this work, we investigated and compared the influence of iron(iii) concentration on the growth of and siderophore production by two heterotrophic bacteria--micrococcus luteus and bacillus silvestris. our results showed that the iron concentration strongly influences the growth of both species. the growth curves were different for each iron concentration and each strain. m. luteus grew more rapidly ...200917689229
evidence-based validation of quorum quenching from lysinibacillus sphaericus and geobacillus sp. in bioremediation of oil sludge.many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (ahl-lactonases) because these enzymes could be used in the control of diseases caused by gram-negative bacteria. the effects of quorum quenching are also remarkable in the performance of bacterial consortia in applications such as bioremediation. in the current work, we demonstrated the presence of a potential novel ahl-lactonase-encoding locus (bsph_3377) from lysinibacillus sphaeri ...201727905820
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