dna relatedness among pseudomonas strains isolated from natural mineral waters and proposal of pseudomonas veronii sp. nov.the taxonomic position of eight strains isolated from mineral water and previously grouped in the authentic pseudomonads on the basis of a phenotypic analysis (cluster ib of m. elomari, l. coroler, d. izard, and h. leclerc [j. appl. bacteriol. 78:71-81, 1995]) has been further studied by dna-dna hybridizations. using the s1 nuclease method at 60 degrees c and labeled reference dna from a representative strain, cfml 92-134, we showed that members of cluster ib constituted a homogeneous group with ...19968863448
intestinal inflammatory pseudotumour with regional lymph node involvement: identification of a new bacterium as the aetiological agent.inflammatory pseudotumours are the morphological expression of diverse processes such as reactive/reparative, infective, and neoplastic. this paper reports an example of intestinal inflammatory pseudotumour, with identification of a newly characterized bacterium in the lesion. the patient presented with intestinal obstruction. laparotomy revealed a tumour in the terminal ileum causing stricture, and multiple enlarged regional lymph nodes. histologically, the tumour and lymph nodes were composed ...200011054710
evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice.of 102 rhizoplane and endophytic bacteria isolated from rice roots and stems in california, 37% significantly (p < or = 0.05) inhibited the growth in vitro of two pathogens, achlya klebsiana and pythium spinosum, causing seedling disease of rice. four endophytic strains were highly effective against seedling disease in growth pouch assays, and these were identified as pseudomonas fluorescens (s3), pseudomonas tolaasii (s20), pseudomonas veronii (s21), and sphingomonas trueperi (s12) by sequencin ...200111718545
pseudomonas mosselii sp. nov., a novel species isolated from clinical specimens.twenty-two fluorescent pseudomonad strains of clinical origin received as pseudomonas fluorescens (10 strains), pseudomonasputida (10 strains) and pseudomonas sp. (2 strains), and 33 type strains of the genus pseudomonas were studied by numerical analysis based on 280 phenotypic characters. twelve of the 22 clinical isolates clustered within a specific group, cluster iv. the other strains clustered within groups containing well-characterized fluorescent pseudomonas species or did not cluster. st ...200211931144
siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads.a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ...200212039729
pseudomonas grimontii sp. nov.the vernacular name 'fluorescent pseudomonas group 97-514' was coined for a group of 43 strains isolated from two french natural mineral waters. all these strains were gram-negative, rod-shaped and motile by means of a single polar flagellum. they produced fluorescent pigment (pyoverdin) on king b medium, catalase and cytochrome oxidase. they were capable of respiratory but not fermentative metabolism. they were not able to accumulate poly-beta-hydroxybutyrate and possessed an arginine dihydrola ...200212361251
pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir.on the basis of phenotypic and genotypic characteristics and 16s rdna sequence analysis, a novel species belonging to the genus pseudomonas sensu stricto was identified. the saprophytic, fluorescent bacterium, designated kmm 3447(t), was isolated from a drinking water reservoir near vladivostok city, russia. the novel organism was a gram-negative, aerobic, rod-shaped bacterium that produced a cyclic depsipeptide with surface-active properties. it degraded casein, but did not degrade gelatin, sta ...200212508877
degradation of 4-amylphenol and 4-hexylphenol by a new activated sludge isolate of pseudomonas veronii and proposal for a new subspecies status.novel pseudomonas strains ina04, ina05, and ina06, were isolated from activated sludge. strain ina06 was found to degrade long chain alkylphenols such as 4-n-amylphenol and 4-n-hexylphenol as the sole source of carbon, apart from co-metabolic degradation of 4-n-nonylphenol in the presence of phenol, while ina04 and ina05 could grow on phenol, but could not grow well on alkylphenols. induction studies on strain ina06 revealed a broad substrate-specific phenol hydroxylase, for the metabolism of ph ...200312576154
a novel catabolic activity of pseudomonas veronii in biotransformation of pentachlorophenol.pseudomonas veronii ph-05, a bacterial strain capable of transforming pentachlorophenol (pcp) to a metabolic intermediate, was isolated by selective enrichment of soil samples from a timber storage yard. strain ph-05 was shown to be able to grow using pcp as the sole source of carbon and energy. gc-ms analysis showed that the metabolic intermediate was tetrachlorocatechol, which inhibited the growth of this strain. the formation of tetrachlorocatechol during biotransformation was monitored, and ...200312883877
[free radicals in mercury-resistant bacteria indicate a novel metabolic pathway].a mercury resistant-soil bacterium p.10.15, identified as a close relative of pseudomonas veronii, was shown to accumulate a specific compound in the stationary phase of growth. this compound is converted to a long-lived free radical under oxidizing conditions, as registered by its epr signal at room temperature. the compound was purified by ion-exchange and gel-filtration chromatography and identified by mass spectroscopy, 2d nmr, and epr as a trisaccharide beta-d-glcpnoh,ch3-(1-->6)-alpha-d-gl ...200314679895
microbial degradation of chlorobenzene under oxygen-limited conditions leads to accumulation of 3-chlorocatechol.five bacterial strains (acidovorax facilis b517, cellulomonas turbata b529, pseudomonas veronii b547, pseudomonas veronii b549, and paenibacillus polymyxa b550) isolated on chlorobenzene as the sole source of carbon and energy were screened for the accumulation of the putative metabolic intermediate 3-chlorocatechol during growth on chlorobenzene under oxygen-limited conditions in the presence and absence of nitrate (1 mm). 3-chlorocatechol accumulated in the growth media of all five strains, bu ...200414982371
microorganisms degrading chlorobenzene via a meta-cleavage pathway harbor highly similar chlorocatechol 2,3-dioxygenase-encoding gene clusters.pseudomonas putida gj31 harbors a degradative pathway for chlorobenzene via meta-cleavage of 3-chlorocatechol. pseudomonads using this route for chlorobenzene degradation, which was previously thought to be generally unproductive, were isolated from various contaminated environments of distant locations. the new isolates, pseudomonas fluorescens sk1 (dsm16274), pseudomonas veronii 16-6a (dsm16273), pseudomonas sp. strain mg61 (dsm16272), harbor a chlorocatechol 2,3-dioxygenase (cbze). the cbze-l ...200415340793
biodegradation of dibenzo-p-dioxin, dibenzofuran, and chlorodibenzo-p-dioxins by pseudomonas veronii ph-03.the dioxin-degrading strain pseudomonas veronii ph-03 was isolated from contaminated soil by selective enrichment techniques. strain ph-03 grew on dibenzo-p-dioxin and dibenzofuran as a sole carbon source. further, 1-chlorodibenzo-p-dioxin, 2-chlorodibenzo-p-dioxin and other dioxin metabolites, salicylic acid, and catechol were also metabolized well. resting cells of strain ph-03 transformed dibenzo-p-dioxin, dibenzofuran, 2,2',3-trihydroxybiphenyl, and some chlorodioxins to their corresponding ...200415523913
[application of molecular systematics to study of bacterial cultures consuming volatile organic compounds].a range of species of four mixed bacterial cultures was studied by molecular systematics methods with the use of 16s rrna genes. the cultures had been developed for application in minireactors, to degrade volatile organic compounds (vocs): ethyl benzene, m-xylene, styrene, and o-xylene. a sample of 30 plasmid rdna clones was obtained for each of the mixed cultures. the clones were analyzed by rflp according to two restriction sites. major variants of the 16s-rdna sequences, corresponding to the ...200515859460
bioaugmentation and enhanced formation of microbial granules used in aerobic wastewater treatment.microbial aggregates of an aerobic granular sludge can be used for the treatment of industrial or municipal wastewater, but their formation from a microbial activated sludge requires several weeks. therefore, the aim of this research was the selection of microbial cultures to shorten the granule-forming period from several weeks to a few days. an enrichment culture with the ability to accelerate granulation was obtained by repeating the selection and batch cultivation of fast-settling microbial ...200616091930
isotopic fractionation indicates anaerobic monochlorobenzene biodegradation.the concentration and isotopic composition of monochlorobenzene (mcb) was monitored in the plume of an anaerobic, contaminated aquifer in bitterfeld, germany. an enrichment in the carbon isotopic composition of more than 4 delta units was found at the fringes of the plume relative to the center (-26.5 %), suggesting the occurrence of in situ biodegradation of mcb. a similar enrichment was measured in a detailed cross-section of the plume and in depth-specific samples obtained in a multilevel sam ...200516117106
pseudomonas peli sp. nov. and pseudomonas borbori sp. nov., isolated from a nitrifying inoculum.sixteen gram-negative, rod-shaped, non-spore-forming isolates were obtained from a nitrifying inoculum. analysis of repetitive sequence-based pcr and sds-page banding patterns, 16s rrna gene sequence analysis and dna-dna hybridizations showed that the isolates belonged to various groups within the genus pseudomonas. one group of isolates could be assigned to pseudomonas migulae and a second to pseudomonas veronii. two groups could be differentiated genotypically from each other and from all othe ...200616902024
cooperative biodegradation of geosmin by a consortium comprising three gram-negative bacteria isolated from the biofilm of a sand filter isolate and identify bacteria from a sand filter column capable of degrading the taste and odour compound, geosmin. in doing so, to investigate if these organisms degrade geosmin either individually or if an alternative mechanism is utilized.200616965373
biodegradation of chlorobenzene under hypoxic and mixed hypoxic-denitrifying conditions.pseudomonas veronii strain ufz b549, acidovorax facilis strain ufz b530, and a community of indigenous groundwater bacteria, adapted to oxygen limitation, were cultivated on chlorobenzene and its metabolites 2-chloro-cis,cis-muconate and acetate/succinate under hypoxic and denitrifying conditions. highly sensitive approaches were used to maintain defined low oxygen partial pressures in an oxygen-re-supplying headspace. with low amounts of oxygen available all cultures converted chlorobenzene, th ...200717279449
degradation of alkyl methyl ketones by pseudomonas veronii mek700.pseudomonas veronii mek700 was isolated from a biotrickling filter cleaning 2-butanone-loaded waste air. the strain is able to grow on 2-butanone and 2-hexanol. the genes for degradation of short chain alkyl methyl ketones were identified by transposon mutagenesis using a newly designed transposon, mini-tn5495, and cloned in escherichia coli. dna sequence analysis of a 15-kb fragment revealed three genes involved in methyl ketone degradation. the deduced amino acid sequence of the first gene, me ...200717351032
microbial community shifts as a response to efficient degradation of chlorobenzene under hypoxic conditions.limitations in the availability of oxygen restrict aerobic biodegradation of chloroaromatic compounds in groundwater ecosystems. in this context the activity of ring-cleaving chlorocatechol dioxygenases (cc12o) is crucial for effective mineralization. previously we demonstrated that oxygen-related enzyme characteristics of cc12o can vary widely among the proteobacteria (balcke et al. submitted). here, we investigated how strains with different ability to transform intermediary 3-chlorocatechol i ...200817882513
kinetics of chlorobenzene biodegradation under reduced oxygen levels.focussing on the role of chlorocatechol 1,2-dioxygenase (cc12o), an oxygen-dependent key enzyme in the aerobic catabolism of chlorobenzene (cb), pseudomonas veronii strain ufz b549, acidovorax facilis strain ufz b530, and a community of indigenous groundwater bacteria were amended with cb degradation under either oxic or hypoxic conditions. all cultures readily degraded cb at high oxygen availability, but had differing abilities to completely degrade cb when exposed to oxygen limitation. for the ...200817934786
functional expression, purification, and characterization of the recombinant baeyer-villiger monooxygenase meka from pseudomonas veronii mek700.for the investigation of the nadph-dependent baeyer-villiger monooxygenase meka from pseudomonas veronii mek700, the encoding gene meka with a c-terminal strep-tag was cloned and expressed under the control of a l: -rhamnose inducible promoter from escherichia coli. the meka gene was found by analyzing the methylethylketone (mek) degradation pathway by onaca et al. j bacteriol 189:3759-3767, 2007. sequence analysis of the corresponding protein, which catalyzes the baeyer-villiger oxidation of me ...200818034235
microbial degradation of chlorinated dioxins.polychlorinated dibenzo-p-dioxins (pcdd) and polychlorinated dibenzofurans (pcdf) were introduced into the biosphere on a large scale as by-products from the manufacture of chlorinated phenols and the incineration of wastes. due to their high toxicity they have been the subject of great public and scientific scrutiny. the evidence in the literature suggests that pcdd/f compounds are subject to biodegradation in the environment as part of the natural chlorine cycle. lower chlorinated dioxins can ...200818083210
cadmium, zinc and copper biosorption mediated by pseudomonas veronii 2e.adsorption properties of bacterial biomass were tested for cd removal from liquid effluents. experimental conditions (ph, time, cellular mass, volume, metal concentration) were studied to develop an efficient biosorption process with free or immobilised cells of pseudomonas veronii 2e. surface fixation was chosen to immobilise cells on inert surfaces including teflon membranes, silicone rubber and polyurethane foam. biosorption experiments were carried out at 32 degrees c and controlled ph; maxi ...200818158237
community-based degradation of 4-chorosalicylate tracked on the single cell level.4-chlorosalicylate (4-cs) can be degraded completely by a bacterial consortium consisting of pseudomonas reinekei (mt1), achromobacter spanius (mt3) and pseudomonas veronii (mt4). the fourth species wautersiella falsenii (mt2) is thought to act as a 'necrotizer' of the community. single cell approaches were used to follow every species' degradation activity within the community by assuming that growth and proliferation are activity markers for the utilization of 4-cs and its degradation pathway ...200818593643
endophytic colonization of potato (solanum tuberosum l.) by a novel competent bacterial endophyte, pseudomonas putida strain p9, and its effect on associated bacterial communities.pseudomonas putida strain p9 is a novel competent endophyte from potato. p9 causes cultivar-dependent suppression of phytophthora infestans. colonization of the rhizoplane and endosphere of potato plants by p9 and its rifampin-resistant derivative p9r was studied. the purposes of this work were to follow the fate of p9 inside growing potato plants and to establish its effect on associated microbial communities. the effects of p9 and p9r inoculation were studied in two separate experiments. the r ...200919329656
pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment.a gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(t)) was isolated from a temporary pond in antarctica. on the basis of 16s rrna gene sequence similarity, strain 14-3(t) was shown to belong to the genus pseudomonas sensu stricto. physiological and biochemical tests supported the phylogenetic affiliation. strain 14-3(t) is closely related to pseudomonas veronii dsm 11331(t), sharing 99.7% sequence similarity. dna-dna hybridization experiments between the two strains sh ...200919688380
introduction of a plasmid-encoded phoa gene for constitutive overproduction of alkaline phosphatase in three subsurface pseudomonas isolates.abstract three bacterial isolates, pseudomonas fluorescens f1, pseudomonas rhodesiae r1 and pseudomonas veronii v1 were genetically modified by introduction of a plasmid, pjh123, with a phoa hybrid gene that directed constitutive overproduction of the enzyme alkaline phosphatase. the presence of the plasmid in the bacterial hosts elevated extracytoplasmic alkaline phosphatase production from 100- to 820-fold. the growth and survival of the plasmid-bearing hosts in sterilized soil slurries was co ...200219709245
chromium (vi) biotransformation by beta- and gamma-proteobacteria from natural polluted environments: a combined biological and chemical treatment for industrial wastes.the high solubility of cr(vi) in aqueous systems, together with carcinogenic and mutagenic effects on living organisms, make industrial effluents receive specific treatments for cr(vi) elimination. biotreatments, based on biotransformation of cr(vi) to cr(iii) which is immobilized as cr(oh)(3), are the most effective methods for the removal of cr(vi) concentrations below 2mm. the aim of our study is the application of pure or mixed bacterial cultures for cr(vi) biotransformation followed by chem ...201019879691
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal.the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ...201020833430
an engineered methanogenic pathway derived from the domains bacteria and archaea.a plasmid-based expression system wherein mekb was fused to a constitutive methanosarcina acetivorans promoter was used to express mekb, a broad-specificity esterase from pseudomonas veronii, in m. acetivorans. the engineered strain had 80-fold greater esterase activity than wild-type m. acetivorans. methyl acetate and methyl propionate esters served as the sole carbon and energy sources, resulting in robust growth and methane formation, with consumption of >97% of the substrates. methanol was u ...201021060738
enzymes hydrolyzing structural components and ferrous ion cause rusty-root symptom on ginseng (panax ginseng).microbial induction of rusty-root was proved in this study. the enzymes hydrolyzing plant structural materials, including pectinase, pectolyase, ligninase, and cellulase, caused the rusty-root in ginseng. pectinase and pectolyase produced the highest rusty-color formation. ferrous ion (fe+++) caused the synergistic effect on rusty-root formation in ginseng when it was used with pectinase. the effect of ferric ion (fe++) on rusty-root formation was slow, compared with fe+++, probably due to gradu ...201121364303
diversity and antagonistic activity of sea ice bacteria isolated from the sea of japan.the aim of the study was to survey cultivable heterotrophic bacteria from sea ice samples collected from peter the great bay of the sea of japan, and to test the isolates for antagonistic activity. a total of 195 strains were isolated and investigated by a phenotypic approach. the 16s rrna gene sequence analysis of thirty eight strains revealed primarily representatives of the class gammaproteobacteria, phylum bacteroidetes, and phylum actinobacteria. members of the class alphaproteobacteria wer ...200821558710
cultured bacterial diversity and human impact on alpine glacier cryoconite.the anthropogenic effect on the microbial communities in alpine glacier cryoconites was investigated by cultivation and physiological characterization of bacteria from six cryoconite samples taken at sites with different amounts of human impact. two hundred and forty seven bacterial isolates were included in actinobacteria (9%, particularly arthrobacter), bacteroidetes (14%, particularly olleya), firmicutes (0.8%), alphaproteobacteria (2%), betaproteobacteria (16%, particularly janthinobacterium ...201121717318
Pyrimidine biosynthesis in Pseudomonas veronii and its regulation by pyrimidines.Pyrimidine biosynthesis in the nutritionally versatile bacterium Pseudomonas veronii ATCC 700474 appeared to be controlled by pyrimidines. When wild type cells were grown on glucose in the presence of uracil, four enzyme activities were depressed while all five enzyme activities increased in succinate-grown cells supplemented with uracil. Independent of carbon source, orotic acid-grown cells elevated aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyltransferase or OMP decarboxyl ...201122112688
differences in culturable microbial communities in bird nestboxes according to orientation and influences on offspring quality in great tits (parus major).although bird-microbial interactions have become a topic of increasing research, the influence of nest-site characteristics, such as cavity orientation, on nest microbial communities in free-living passerines has not, to our knowledge, been investigated. this is despite the possibility of microbial differences explaining non-random patterns in nest-site selection and offspring quality, such as those exhibited by great tits (parus major). we swabbed great tit nestboxes that faced either south-sou ...201122183046
engineering pseudomonas protegens pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.nitrogen is the second most critical factor for crop production after water. in this study, the beneficial rhizobacterium pseudomonas protegens pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of pseudomonas stutzeri a1501 via the x940 cosmid. pf-5 x940 was able to grow in l medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. pf-5 x940 also showed constitutive expression and enzymatic ac ...201323675499
draft genome sequence of pseudomonas veronii strain 1ydbtex2.pseudomonas veronii strain 1ydbtex2 was isolated from a benzene-contaminated site. here we report the draft genome sequence of 1ydbtex2 and its genes associated with aromatic metabolism. the broad catabolic potential of this strain is consistent with the environment from which it was isolated.201323682152
degradation of benzene by pseudomonas veronii 1ydbtex2 and 1yb2 is catalyzed by enzymes encoded in distinct catabolism gene clusters.pseudomonas veronii 1ydbtex2, a benzene and toluene degrader, and pseudomonas veronii 1yb2, a benzene degrader, have previously been shown to be key players in a benzene-contaminated site. these strains harbor unique catabolic pathways for the degradation of benzene comprising a gene cluster encoding an isopropylbenzene dioxygenase where genes encoding downstream enzymes were interrupted by stop codons. extradiol dioxygenases were recruited from gene clusters comprising genes encoding a 2-hydrox ...201526475106
pseudomonads rule degradation of polyaromatic hydrocarbons in aerated sediment.given that the degradation of aromatic pollutants in anaerobic environments such as sediment is generally very slow, aeration could be an efficient bioremediation option. using stable isotope probing (sip) coupled with pyrosequencing analysis of 16s rrna genes, we identified naphthalene-utilizing populations in aerated polyaromatic hydrocarbon (pah)-polluted sediment. the results showed that naphthalene was metabolized at both 10 and 20°c following oxygen delivery, with increased degradation at ...201526635740
the genome of the toluene-degrading pseudomonas veronii strain 1ydbtex2 and its differential gene expression in contaminated sand.the natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (btex) may be accelerated by inoculation of specific biodegraders (bioaugmentation). bioaugmentation mainly involves introducing bacteria that deploy their metabolic properties and adaptation potential to survive and propagate in the contaminated environment by degrading the pollutant. in order to better understand the adaptive response of cells during a transition to con ...201627812150
a draft genome sequence of pseudomonas veronii r4: a grapevine (vitis vinifera l.) root-associated strain with high biocontrol potential.a new plant commensal pseudomonas veronii isolate (strain r4) was identified from a xiphinema index biocontrol screen. isolated from grapevine roots from vineyards in central chile, the strain r4 exhibited a slower yet equivalently effective nematicide activity as the well-characterized p. protegens cha0. whole genome sequencing of strain r4 and comparative analysis among the available pseudomonas spp. genomes allowed for the identification of gene clusters that encode putative extracellular pro ...201627777646
ancient bacteria of the ötzi's microbiome: a genomic tale from the copper age.ancient microbiota information represents an important resource to evaluate bacterial evolution and to explore the biological spread of infectious diseases in history. the soft tissue of frozen mummified humans, such as the tyrolean iceman, has been shown to contain bacterial dna that is suitable for population profiling of the prehistoric bacteria that colonized such ancient human hosts.201728095919
biogenic nanoparticles bearing antibacterial activity and their synergistic effect with broad spectrum antibiotics: emerging strategy to combat drug resistant pathogens.the present study emphasizes on synthesis of bimetallic silver-gold nanoparticles from cell free supernatant of pseudomonas veronii strain as41g inhabiting annona squamosa l. the synthesized nanoparticles were characterized using hyphenated techniques with uv-visible spectra ascertained absorbance peak between 400 and 800 nm. possible interaction of biomolecules in mediating and stabilization of nanoparticles was depicted with fourier transform infrared spectroscopy (ftir). x-ray diffraction (xr ...201728223861
novel components of leaf bacterial communities of field-grown tomato plants and their potential for plant growth promotion and biocontrol of tomato diseases.this work aimed to characterize potentially endophytic culturable bacteria from leaves of cultivated tomato and analyze their potential for growth promotion and biocontrol of diseases caused by botrytis cinerea and pseudomonas syringae. bacteria were obtained from inner tissues of surface-disinfected tomato leaves of field-grown plants. analysis of 16s rrna gene sequences identified bacterial isolates related to exiguobacterium aurantiacum (isolates bt3 and mt8), exiguobacterium spp. (isolate gt ...201626654914
microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using 16s rrna gene analysis.sea bream fillets are one of the most important value-added products of the seafood market. fresh seafood spoils mainly owing to bacterial action. in this study an exploration of initial and spoilage microbiota of sea bream fillets stored under air and commercial modified atmosphere packaging (map) at 0 and 5 °c was conducted by 16s rrna gene sequence analysis of isolates grown on plates. sensory evaluation and enumeration of total viable counts and spoilage microorganisms were also conducted to ...201525312872
uptake of carbamazepine by rhizomes and endophytic bacteria of phragmites australis.carbamazepine is an antiepileptic and mood-stabilizing drug which is used widely in europe and north america. in the environment, it is found as a persistent and recalcitrant contaminant, being one of the most prominent hazardous pharmaceuticals and personal care products in effluents of wastewater treatment plants. phragmites australis is one of the species with both, the highest potential of detoxification and phytoremediation. it has been used successfully in the treatment of industrial and mun ...201525750647
bacterial swimming, swarming and chemotactic response to heavy metal presence: which could be the influence on wastewater biotreatment efficiency?fixed-bed reactors are usually designed for wastewater biotreatments, where the biofilm establishment and maintenance play the most important roles. biofilm development strictly relies on different types of bacterial motility: swimming, swarming, and chemotaxis, which can be altered by the microenvironment conditions. the aim of this work is to do an integrated study on the effects of cu(ii), cd(ii), zn(ii) and cr(vi) on swimming, swarming and chemotaxis of pseudomonas veronii 2e, delftia acidov ...201222806721
molecular characterization of spoilage bacteria as a means to observe the microbiological quality of carrot.this study characterized the bacteria causing decay of carrots during storage and marketing. spoilage strains were identified by 16s-amplified rdna restriction analysis and intergenic transcribed spacer-pcr-restriction fragment length polymorphism (its-pcr-rflp). genotypic fingerprinting by rflp-pulsed-field gel electrophoresis was used to assess the genetic diversity of the isolates. a total of 252 pseudomonas isolates from carrots were identified and classified into eight separate groups. most ...201222410227
functional characterization and application of a tightly regulated mekr/p meka expression system in escherichia coli and pseudomonas putida.a methyl ethyl ketone (mek)-inducible system based on the broad-host-range plasmid pbbr1mcs2 and on the p meka promoter region of the mek degradation operon of pseudomonas veronii mek700 was characterized in escherichia coli jm109 and pseudomonas putida kt2440. for validation, β-galactosidase (lacz) was used as a reporter. the novel system, which is positively regulated by mekr, a member of the arac/xyls family of regulators, was shown to be subject to carbon catabolite repression by glucose, wh ...201323771781
pseudomonas lactis sp. nov. and pseudomonas paralactis sp. nov., isolated from bovine raw milk.five strains, designated ws 4672t, ws 4998, ws 4992t, ws 4997, and ws 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. the most similar species on the basis of 16s rrna gene sequences were pseudomonas azotoformans iam 1603t, pseudomonas gessardii cip 105469t, and pseudomonas libanensis cip 105460t showing 99.7-99.6 % similarity. using rpod gene sequences pseudomonas veronii lmg 17761t (93.3 %) was most closely related to strain ws 4672t and pseudomonas ...201728141500
long-chain alkyl cyanides: unprecedented volatile compounds released by pseudomonas and micromonospora bacteria.the analysis of volatiles from bacterial cultures revealed long-chain aliphatic nitriles, a new class of natural products. such nitriles are produced by both gram-positive micromonospora echinospora and gram-negative pseudomonas veronii bacteria, although the structures differ. a variable sequence of chain elongation and dehydration in the fatty acid biosynthesis leads to either unbranched saturated or unsaturated nitriles with an ω-7 double bond, such as (z)-11-octadecenenitrile, or methyl-bran ...201728276609
candidate nematicidal proteins in a new pseudomonas veronii isolate identified by its antagonistic properties against xiphinema index.the nematode xiphinema index affects grape vines and transmits important viruses associated with fanleaf degeneration. pseudomonas spp. are an extensive bacterial group in which important biodegradation and/or biocontrol properties can occur for several strains in the group. the aim of this study was to identify new pseudomonas isolates with antagonist activity against x. index. forty bacterial isolates were obtained from soil and root samples from chilean vineyards. thirteen new fluorescent pse ...201727989999
production of a bioflocculant from pseudomonas veronii l918 using the hydrolyzate of peanut hull and its application in the treatment of ash-flushing wastewater generated from coal fired power this study, bioflocculant produced by pseudomonas veronii l918 was applied to treat ash-flushing wastewater. the strain l918 could convert the hydrolyzate of peanut hull into bioflocculant, which can effectively reduce the production cost of bioflocculant. the yield of 3.39g/l bioflocculant mbf-l918 was achieved when 300ml/l peanut hull hydrolyzate was used as carbon source. the bioflocculant mbf-l918 contains 77.14% polysaccharides and 4.84% proteins, and the molecular weight (mw) of mbf-l91 ...201627372012
engineering of corynebacterium glutamicum to utilize methyl acetate, a potential feedstock derived by carbonylation of methanol with co.the possibilities to utilize one-carbon substrates (c1) like co, methane and methanol have been explored as a cheap alternative feedstock in the biotechnology. for the first time, methyl acetate (meoac), which can be formed from carbonylation of methanol with co, was demonstrated to be an alternative carbon source for the cell growth of corynebacterium glutamicum as a model microbial cell factory. to do so, a carboxyl esterase activity was necessary to hydrolyze meoac to methanol and acetate. al ...201626970052
long-term salinity tolerance is accompanied by major restructuring of the coral bacterial microbiome.scleractinian corals are assumed to be stenohaline osmoconformers, although they are frequently subjected to variations in seawater salinity due to precipitation, freshwater run-off and other processes. observed responses to altered salinity levels include differences in photosynthetic performance, respiration and increased bleaching and mortality of the coral host and its algal symbiont, but a study looking at bacterial community changes is lacking. here, we exposed the coral fungia granulosa t ...201626840035
a novel esterase subfamily with α/β-hydrolase fold suggested by structures of two bacterial enzymes homologous to l-homoserine o-acetyl transferases.mekb from pseudomonas veronii and cghle from corynebacteriumglutamicum belong to the superfamily of α/β-hydrolase fold proteins. based on sequence comparisons, they are annotated as homoserine transacetylases in popular databases like uniprot, pfam or esther. however, experimentally, mekb and cghle were shown to be esterases that hydrolyse preferentially acetic acid esters. we describe the x-ray structures of these enzymes solved to high resolution. the overall structures confirm the close relat ...201626787467
comparison of differential gene expression to water stress among bacteria with relevant pollutant-degradation properties.resistance to semi-dry environments has been considered a crucial trait for superior growth and survival of strains used for bioaugmentation in contaminated soils. in order to compare water stress programmes, we analyse differential gene expression among three phylogenetically different strains capable of aromatic compound degradation: arthrobacter chlorophenolicus a6, sphingomonas wittichii rw1 and pseudomonas veronii  1ydbtex2. standardized laboratory-induced water stress was imposed by shock ...201626616826
biosynthesis of gold nanoparticles by pseudomonas veronii as41g inhabiting annona squamosa l.biogenic principles to nanotechnology have generated tremendous attention in recent past owing eco friendly benign process for synthesis of nanoparticles. present investigation reports extracellular synthesis of gold nanoparticles using cell free supernatant of pseudomonas veronii as 41g, a novel endophyte isolated from annona squamosa l. gold nanoparticles formation was confirmed with uv-visible spectrophotometer. ftir analysis predicted various functional groups responsible for reduction of me ...201526093965
extracellular synthesis of silver nanoparticles by novel pseudomonas veronii as41g inhabiting annona squamosa l. and their bactericidal present investigation extracellular synthesis of silver nanoparticles were synthesized using cell free supernatant of pseudomonas veronii as41g isolated from annona squamosa l. the bacterium significantly reduced silver nitrate to generate silver nanoparticles which was characterized with hyphenated techniques. synthesis of silver nanoparticles preliminary confirmed by uv-visible spectrophotometry with the intense peak at 410nm, further ftir analysis revealed the possible role of biomolecules ...201525459703
kinetic analysis and bacterium metabolization of alpha-pinene by a novel identified pseudomonas sp. strain.biodegradation has become a popular alternative remediation technology for its economic and ecological advantages. an aerobic bacterium (strain zw) capable of degrading alpha-pinene was isolated from a biofilter by a selective enrichment. based on the 16s rrna gene analysis and physiochemical properties, this strain was identified as pseudomonas veronii. under the optimized condition achieved by the response surface methodology (rsm), as well as ph 6.82, temperature 26.3 degrees c and nacl conce ...201223520851
an innovative approach to bioremediation of mercury contaminated soils from industrial mining operations.soils contaminated with mercury (hg) have proved expensive and logistically difficult to remediate. research continues into finding suitable environmentally-friendly and efficient ways of achieving this end. bioremediation is an option, which employs the strategies microorganisms have evolved to deal with hg. one microbial strategy involves uptake and intracellular volatilisation of mercuric ions, which passively diffuse from the cell and back into the atmosphere. in this work, pseudomonas veron ...201728633064
screening for biosurfactant production by 2,4,6-trinitrotoluene-transforming isolate and identify tnt-transforming cultures from explosive-contaminated soils with the ability to produce biosurfactants.201728561275
effect of phosphorus addition on the reductive transformation of pentachlorophenol (pcp) and iron reduction with microorganism involvement.the transformation of phosphorus added to the soil environment has been proven to be influenced by the fe biochemical process, which thereby may affect the transformation of organic chlorinated contaminants. however, the amount of related literatures regarding this topic is limited. this study aimed to determine the effects of phosphorus addition on pentachlorophenol (pcp) anaerobic transformation, iron reduction, and paddy soil microbial community structure. results showed that the transformati ...201728444568
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