somatic antigens of pseudomonas aeruginosa. the structure of o-specific polysaccharide chains of the lipopolysaccharides from p. aeruginosa o5 (lányi) and immunotype 6 (fisher).lipopolysaccharides were isolated from dry bacterial cells of pseudomonas aeruginosa o5a,b,c, o5a,b,d, o5a,d (lányi classification) and immunotype 6 (fisher classification) by the westphal procedure. their polysaccharide chains were built up of trisaccharide repeating units containing d-xylose, 2-acetamido-2,6-dideoxy-d-galactose and a new sialic acid-like sugar, the di-n-acyl derivative of 5,7-diamino-3,5,7,9-tetradeoxy-l-glycero-l-manno-nonulosonic (pseudaminic) acid. formyl, acetyl and (r)-3- ...19873104041
[prevention using a pseudomonas immunoglobulin in burn patients].pseudomonas aeruginosa belongs to the most frequent pathogens isolated from patients with burns. in a mouse model for artificial burns it was found that prophylactic administration of a hyperimmune globulin with antibody titres against p. aeruginosa (fisher immunotypes 1, 2, 4 and 6) reduced mortality. therefore, the prophylactic administration of pseudomonas immunoglobulin was examined in a prospective randomized study in two groups of 13 patients each. severely burned patients with at least se ...19873106234
production of cytotoxin by clinical strains of pseudomonas aeruginosa.presence of cytotoxin was studied in extracts of 57 strains of pseudomonas aeruginosa (46 bacteremia, 4 environmental, and 7 fisher immunotype), 10 pseudomonas species, and 7 nonpseudomonas isolates. cytotoxin was identified by western immunoblot in extracts of all p. aeruginosa isolates. none of the pseudomonas species or nonpseudomonas isolates were shown to produce this protein. no immunologic cross-reactivity was observed between cytotoxin antibody and p. aeruginosa alkaline protease, toxin ...19873107788
comparison of the chinese schema and the international antigenic typing system for serotyping pseudomonas aeruginosa.twelve strains of pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the people's republic of china were compared with serogroups in the international antigenic typing system (iats). the first eight groups originated in the people's republic of china, and group ii appears to have a new major antigen that is not found in the iats. groups i, iii, iv, v, vi, vii, and viii correspond to groups 11, 6, 9, 4, 8, 3, and 1, respectively, of the iats. groups ix, x, xi, an ...19873108310
[pseudomonas immunoglobulin prophylaxis in patients with burn injuries].pseudomonas aeruginosa belongs to the most frequent pathogens isolated from patients with burns. in a mouse model for artificial burns it was found that prophylactic administration of a hyperimmune globulin with antibody titres against p. aeruginosa (fisher immunotypes 1, 2, 4 and 6) reduced mortality. therefore, the prophylactic administration of pseudomonas immunoglobulin was examined in a prospective randomized study in two groups of 13 patients each. severely burned patients with at least se ...19873112025
dose requirements, assay procedures and tissue specificity for pcb inductation of p-450 dependent mono-oxygenase activity in the rat: implications for design of studies measuring in vivo induction of human placental mono-oxygenases.pregnant sprague-dawley and fisher 344 rats were treated on day 15 of gestation with aroclor 1254 in a single dose ranging from 0 to 500 mg kg-1 body weight and killed on day 18 of gestation. in the small groups of animals used for this study, no effect was observed on mean maternal liver or placental weight, or the number of fetal resorptions at any of the doses tested. measurement of aryl hydrocarbon hydroxylase (ahh) and 7-ethoxycoumarin o-deethylase (7ecd) activity in tissue homogenates, how ...19873114357
the role of humoral immunity in lactobacillus casei cell wall induced arthritis.although both cellular and humoral immunity have been shown to play important roles in the development of collagen induced arthritis, their roles in the pathogenesis of bacterial cell wall induced arthritis remain unclear. previous studies of humoral immunity in bacterial cell wall induced arthritis were carried out in outbred animals and measured the humoral response to only the eliciting bacteria. we compared the humoral immune responses of arthritis susceptible lewis and arthritis resistant f ...19873114482
somatic antigens of pseudomonas aeruginosa. the structure of o-specific polysaccharide chains of lipopolysaccharides of p. aeruginosa o3 (lányi), o25 (wokatsch) and fisher immunotypes 3 and 7.o-specific polysaccharides, obtained on mild acid degradation of lipopolysacchrides of the serologically related strains pseudomonas aeruginosa o3 (lányi classification), o25 (wokatsch classification) and immunotypes 3 and 7 (fisher classification), are built up of trisaccharide repeating units involving 2-acetamido-2,6-dideoxy-d-galactose (n-acetyl-d-fucosamine), 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid or 2,3-diacetamido-2,3-dideoxy-l-guluronic acid and 3-acetamidino-2-acetamido-2,3-dideo ...19873115777
hemagglutination patterns of pseudomonas aeruginosa strains of different serogroups, habs and fisher types.pseudomonas aeruginosa strains of sixteen habs and three fisher types were examined for their hemagglutination activity using vertebrate erythrocytes including human ones. their carbohydrate-binding specificity as determined by hemagglutination-inhibition suggests a possible method of their purification. this study also indicates the possibility of an occurrence of multiple lectins of different specificity.19873118598
induction of rat thyroid cell mhc class ii antigen by thyrotropin and gamma-interferon.major histocompatibility (mhc) class ii antigen expression by thyroid epithelial cells has been widely implicated in the pathogenesis of autoimmune thyroid disease. we have examined rat mhc (rt1) class ii antigen gene regulation in 1b-6 cells, cloned in our laboratory from the fisher rat thyroid line frt-l5. 1b-6 cells are tsh dependent for growth and proliferation, and are responsive to more than 5 microu/ml bovine tsh (btsh) in terms of extracellular camp accumulation. recombinant rat gamma in ...19873119313
characterization of proteoglycans synthesized by rat thyroid cells in culture and their response to thyroid-stimulating hormone.a fisher rat thyroid cell line was maintained in culture and the cells were labeled with [3h]glucosamine, [35s]sulfate, and [35s]cysteine to examine the synthesis of proteoglycans. 3h and 35s radioactivity from these precursors were incorporated into both chondroitin sulfate (cs) and heparan sulfate (hs) proteoglycans. cs proteoglycans were almost exclusively secreted into the medium while hs proteoglycans remained mainly associated with the cell layer. single chain glycosaminoglycans released b ...19883123476
the intestinal zinc transport in aged determine if there are any age-related alterations in the intestinal zinc absorption that may contribute to zinc deficiency, we studied the zinc transport across the jejunal segments of 3-, 12- and 24-month-old fisher 344 male rats using the ussing chamber technique. we also evaluated the effect of 5 microm arachidonic acid in tissue bathing media. the zinc transport from mucosa to serosa in 24-month-old rats (55.0 +/- 3.5 nmol/h/cm2) was significantly greater than the transport in 12-month-o ...19873123812
enhancement of udp-glucuronyltransferase, udp-glucose dehydrogenase, and glutathione s-transferase activities in rat liver by dietary administration of eugenol.male fisher rats were fed a diet ad lib. containing eugenol (4-allyl-2-methoxyphenol) to observe its effects on liver drug-detoxifying enzymes such as udp-glucuronyltransferase (gt), udp-glucose dehydrogenase (dh) and glutathione s-transferase (gst). liver weights were not affected significantly by a diet containing 3% eugenol (w/w) for 13 weeks. the activities of gt of liver microsomes toward various xenobiotic substances such as 4-nitrophenol, 1-naphthol, 4-hydroxybiphenyl and 4-methylumbellif ...19883125837
x-linked immunodeficient mice as a model for testing the protective efficacy of monoclonal antibodies against pseudomonas aeruginosa.(dba/n[female] x cba/2[male])f1 males have been reported to be deficient in producing antibodies against a number of antigens, including carbohydrates (i. scher, adv. immunol. 35:1-71, 1982). we show that f1 male mice, in contrast to females, made less lipopolysaccharide (lps)-specific antibodies after immunization with heat-inactivated pseudomonas aeruginosa and had significantly less naturally occurring lps-specific antibodies. furthermore, neutropenic males were 50 to 1,000 times more sensiti ...19883128480
aurothioglucose effect on sulfhydryls and glutathione-metabolizing enzymes: in vivo inhibition of selenium-dependent glutathione peroxidase.fisher 344 rats injected with a total of 20.8 +/- 1.5 mg of gold (au) as aurothioglucose over an 8-wk period were used to study the effect of long-term au treatment on selenium-dependent glutathione peroxidase (segshpx), other enzymes related to gsh metabolism, gsh, nonprotein sulfhydryls, and total sulfhydryls (sh) in various tissues. the indirect coupled assay for segshpx revealed decreased activity in platelets of au-treated rats but not in other tissues. inhibition of segshpx by au is revers ...19883128851
specific and nonspecific imaging of localized fisher immunotype 1 pseudomonas aeruginosa infection with radiolabeled monoclonal determine if radiolabeled specific antibodies directed against bacterial antigens could be used to detect sites of infection, gamma camera imaging studies were performed in animals infected with pseudomonas aeruginosa. murine monoclonal antibodies (mabs) directed against fisher immunotype 1 pseudomonas aeruginosa and a nonmicrobial, nonmammalian haptene, p-arsanilic acid, were labeled with 125i by the lodogen-bead method. unilateral, deep thigh infections were created by innoculation with 2 x ...19883131499
use of l-glutamine in total parenteral nutrition.gut atrophy develops during prolonged total parenteral nutrition (tpn). tpn solutions do not contain glutamine, an energy substrate of the intestinal tract. this study evaluated the effect of addition of l-glutamine to tpn on gut nitrogen content, histology, and disaccharidase enzyme activity. five groups of six fisher 344 rats received rat chow, d5w, tpn (23% calories as lipid), or tpn with 1 or 2% l-glutamine. animals given tpn received 30 kcal and 0.22 g nitrogen/100 g/day. metabolic cages al ...19883131588
biliary secretory iga levels in rats with protein-calorie malnutrition.malnutrition is a recognized cause of failure of host defense mechanisms. in the past 5 years, it has been demonstrated that the gut, long known to have significant morphologic changes with protein calorie malnutrition (pcm), is an immune organ affected by malnutrition. to assess the role of biliary immunoglobulin a (s-iga), part of the barrier to bacterial invasion from the gastrointestinal tract, the following study was performed. seventy-eight fisher female inbred rats weighing 110-130 g were ...19883132112
comparison of stainless steel penicylinders used in disinfectant testing.two brands of stainless steel penicylinders, s&l metal products and fisher scientific, were simultaneously tested to determine if they provide comparable results when used in the aoac use-dilution method of disinfectant testing. results showed consistently more positive tubes for the fisher brand of penicylinders than for the s&l, regardless of the surface finish of the test cylinders.19883133351
some aspects of the diagnosis of specific vaginal infections in the rotterdam std clinic population.we studied specific vaginal infections in 351 consecutive women who visited the rotterdam std clinic. prostitutes less often had vaginal or vulvar complaints than non-prostitutes (fisher test, p less than 0.01), but more often had abnormal discharge (fisher test, p less than 0.001). normal secretions were found in 52% of the women. clue cell-positive discharge (ccpd) was by far the most prevalent vaginal disease entity (32%). the wet mount showed pseudo-hyphae in 14 women (4%) and trichomonas va ...19883134262
the influence of tobramycin dosage regimens on nephrotoxicity, ototoxicity, and antibacterial efficacy in a rat model of subcutaneous abscess.the influence of dosage regimen on the nephrotoxicity, ototoxicity, and antibacterial efficacy of tobramycin was assessed in fisher rats with pseudomonas aeruginosa subcutaneous abscesses. a subcutaneous tobramycin dose of 10 mg/kg every 4 h resulted in peak and trough serum concentrations that approximated those currently recommended for patients. subsequently, the influence of this subcutaneous dosage regimen was compared with three other regimens that administered the same total daily dose: 2 ...19883134490
tumor localization of newly developed hematoporphyrin (dhp) using a bladder tumor model: a novel hematoporphyrin derivative.application of laser irradiation with porphyrin(s) or their derivatives for the destruction of tumors in humans requires preliminary studies of their localization in normal and malignant tissues. a novel derivative of hematoporphyrin (hp) was prepared. the newly developed hematoporphyrin (dhp) was administered to fisher rats with bladder tumors and showed greater accumulation in the tumoral tissues. comparative data on (hp) and (dhp) are presented and discussed in light of the enhanced tumor por ...19883134948
human monoclonal antibodies that protect mice against challenge with pseudomonas aeruginosa.lymphocytes from healthy volunteers and from cystic fibrosis patients were transformed with epstein-barr virus and cultured at a limiting dilution to generate lymphoblastoid cell lines that secreted human monoclonal antibodies specific for lipopolysaccharide (lps) from pseudomonas aeruginosa. three cell lines (rm5, fdd7, and 11f9) produced immunoglobulin m (igm) antibody species that reacted specifically with p. aeruginosa fisher immunotypes 2, 4, and 5, respectively, and with lps extracted from ...19883135264
structure of an extracellular cross-reactive polysaccharide from pseudomonas aeruginosa immunotype 4.a neutral small molecular mass (approximately 6.5 kda) polysaccharide comprising a pentasaccharide repeat unit was isolated from culture supernatants of pseudomonas aeruginosa immunotype 4. the polysaccharide had a pentasaccharide repeating unit as follows (formula; see text) where rha is rhamnose. the structure was determined using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride, methylation analysis, and 1h and 13c nuclear magnetic resonance spectroscopy including nuclear overhaus ...19883136157
apomorphine doses impair the reaction time of fast reacting but not slow reacting rats.previously, several studies in our laboratories have linked the ability to initiate movement rapidly (reactive capacity) in several rat strains to markers of nigrostriatal dopamine function. in the present investigation we wished to determine the extent to which fast-reacting (fr) and slow-reacting (sr) rats responded differentially to the effects of dopamine agonist (apomorphine) administration. fisher 344 rats were operantly conditioned on a shock-avoidance, reactive capacity task which requir ...19883137611
effect of chronic aurothioglucose treatment of rats on kidney catalase and cytochromes.catalase activity and cytochrome content were measured in kidneys of fisher 344 rats injected with aurothioglucose (atg) either daily for 3 days or 5 days a week for up to 8 wk. catalase activity was decreased 39%, 59%, and 48% (all p less than 0.001) after 3 days, 2 wk, and 8 wk, respectively. microsomal cytochrome p-450 levels decreased 71%, 86%, and 80% (all p less than 0.001) after 3 days, 2 wk, and 8 wk, respectively. in contrast, cytochrome b5 was significantly increased at 3 days and 2 wk ...19883138383
mechanisms of reduction of tumor recurrence with carbon dioxide laser in experimental mammary tumors.this study compares local tumor recurrence after low energy co2 laser wound sterilization with recurrence after scalpel, laser or electrocautery excision. wound histologic changes were studied to understand the mechanism of the interaction between the laser and wound. single implants of r3230ac mammary tumor were grown to an average diameter of 24 millimeters in the mammary ridge of 80 female fisher 344 rats. rats were anesthesized with pentobarbital and randomized into groups, each with similar ...19883142066
lambda phage cro repressor interaction with its operator dna: 2'-deoxy-5-fluorouracil or3 analogues.the experiments here show that chemically synthesized dna containing fluorine at selected sites can be used to test specific predictions of a model for cro repressor--operator interaction. this is done by observation of the perturbation to the fluorine-19 nmr spectra of analogues of or3 synthesized with 2'-deoxy-5-fluorouracil at specific positions in the dna helix. although the three-dimensional structure of the cro repressor from phage lambda has been determined by matthews and co-workers [and ...19853157402
the autologous mixed lymphocyte reaction is decreased in freund's adjuvant-injected rats of arthritis-susceptible and -insusceptible strains.we examined the t-non-t cell autologous mixed lymphocyte reaction (amlr) of spleen cells from rats with arthritis induced by freund's complete adjuvant in an effort to establish an animal model for the study of the relationship between the amlr and autoimmune disease. we found that the splenic t-non-t amlr was markedly decreased in rats with adjuvant-induced arthritis and that this decrease was mediated by suppressor cells within the nylon-wool-adherent stimulator cell population. however, we al ...19853159391
identification of the transformation-associated cell surface antigen expressed on the rat fetus-derived fibroblast.a wka rat fetus-derived fibroblast cell line wfb showed strict nontransformant phenotypes in vitro such as anchorage dependency of cell growth in soft agar, contact inhibition, and serum dependency on the monolayer cell culture. transfection of 6.6-kilobase ejras oncogene into wfb resulted in the acquisition of tumorigenicity in vitro and in vivo. the cell surface antigen that is moderately or highly expressed on these wfb transformants, designated as w14 and w31, was analyzed using monoclonal a ...19883162824
amino acid profiles in tumor-bearing and pair-fed nontumor-bearing malnourished investigate the metabolic and organ changes accompanying growth of a malignant tumor, ten male fisher 344 rats weighing 150 to 200 g were inoculated subcutaneously with 10(6) viable mca sarcoma cells (tumor-bearing). ten other rats (controls) were similarly inoculated with saline. both groups were allowed food and water ad libitum. an additional ten rats (pair-fed) were inoculated with saline and fed the same mean daily food intake as the tumor-bearing rats. thirty-five days after inoculation ...19883167766
bile salt-dependent, neutral cholesteryl ester hydrolase of rat liver: possible relationship with pancreatic cholesteryl ester hydrolase.homogenates of the livers of outbred, sprague-dawley rats contain a neutral cholesteryl ester hydrolase activity that requires millimolar concentrations of bile salts for maximal activity. previous studies showed that this activity had the unusual property of being highly variable among individual rats. the present studies were conducted to define further the nature of this enzymic activity and to explore the possible basis for the variability. individual liver homogenates from inbred fisher-344 ...19883179327
synthesis and kinetics of decomposition of 7-(2-cyanoethyl)guanine and o6-(2-cyanoethyl)guanine, markers for reaction of acrylonitrile and 3-(methylnitrosamino)propionitrile with dna.acrylonitrile, a carcinogen in rodents, is used in the large-scale production of acrylic polymers. 3-(methylnitrosamino)propionitrile, a potent carcinogen in fisher rats, is an areca-derived nitrosamine which has been found in the saliva of betel quid chewers. upon metabolic activation, both of these carcinogens can react with dna to form 7-(2-cyanoethyl)guanine and o6-(2-cyanoethyl)guanine. the latter has never been described. we have therefore synthesized this derivative by reacting 2-(n-carbe ...19883180348
influence of dietary vitamin e on the intermembrane transfer of alpha-tocopherol as mediated by an alpha-tocopherol binding protein.the effect of dietary vitamin e on the intermembrane transfer of (3r)-alpha-tocopherol, a spontaneous process accelerated in the presence of an alpha-tocopherol binding protein (alpha tbp), was examined. the transfer activity of this cytosolic liver protein was assayed via in vitro transfer of (3r)-alpha-[3h]tocopherol (alpha[3h]t) from egg lecithin liposomes to human erythrocyte ghosts (eg). male fisher 344 rats (1 and 20 months old) were fed diets containing 0, 30, and 500 mg/kg vitamin e (dl- ...19883186764
chronic tubulointerstitial nephritis and renal insufficiency associated with long-term "subtherapeutic" determine whether long-term "subtherapeutic" concentrations of aminoglycoside produce chronic tubulointerstitial nephropathy, fisher rats were given gentamicin, 20 mg/kg/day, for up to 6 months via indwelling osmotic infusion pumps. studies included renal histology, autoradiographic quantitation of renal cell tritiated thymidine uptake, renal function and renal cortical gentamicin assay. acute proximal tubular injury, without tubular necrosis, followed by recovery, occurred during the first m ...19883193025
boron-11 mri and mrs of intact animals infused with a boron neutron capture agent.boron neutron capture therapy (bnct) depends on the delivery of boron-containing drugs to a targeted lesion. currently, the verification and quantification of in vivo boron content is a difficult problem. boron-11 spectroscopy was utilized to confirm the presence of a dimeric sulfhydryl dodecaborane bnct agent contained in an intact animal. spectroscopy experiments revealed that the decay time of transverse magnetization of the boron-11 spins was less than 1 ms which precluded the use of a 2dft ...19883210959
the investigation of experimental brain tumours using 31p-mrs and vivo 31p-magnetic resonance spectra (mrs) were obtained by the surface coil method from rat glioma, human glioblastoma, and human neuroblastoma inoculated subcutaneously in cd fisher rats and hamsters, and the effects of chemotherapy, photoradiation therapy, and radiofrequency hyperthermia as well as 60co-irradiation were evaluated by sequentially observing spectral changes. in the 31p-spectra of tumour tissue, the nucleoside triphosphate (ntp), phosphomonoesters (pme) peaks were high and the ...19883213641
comparison of local tumor recurrence following excision with the co2 laser, nd:yag laser, and argon beam coagulator.this study compares the incidence of local tumor recurrence following primary excision with the co2 laser, nd:yag laser (contact), argon beam coagulator, or electrocautery. one hundred eight fisher 344 rats with r3230ac mammary tumors (1.6 +/- 0.04 [sd] cm diameter) were used. all animals were randomized into groups of similar tumor size. in groups c and cs, excision was performed with a sharplan 1060 co2 laser (temoo, 25 w, continuous wave [cw], 0.2-mm spot size). wounds in group cs were "steri ...19883230999
effects of short-term dietary restriction on survival of mammary ascites tumor-bearing rats.we studied the effects of short-term dietary restriction on the survival of 3-4-month-old tumor-free and tumor-bearing fisher rats. the diet-restricted food regimen consisted of alternate day ad libitum feeding followed by alternate day fasting. diet-unrestricted control rats were fed ad libitum daily. six tumor-free rats on the diet-restricted regimen compensated for the dietary restriction by an increase in food consumption during the alternate feeding days, and lost an average of only 2-3% of ...19883245934
[correlation between nasopharyngeal carcinoma and hla in sichuan].fifty three cases of nasopharyngeal carcinoma (npc) in sichuan and 212 normal individuals in our university were studied for their human leucocyte antigen(hla) typing by the standard nih microcytotoxicity test. a total of 135 antisera which could defined 44 specificities of hla-a, b and dr loci were used for this purpose. in this paper, bw46 and dr antigens for detecting npc patients is reported at home for the first time. the results showed that there existed a strong correlation between bw46 a ...19883248481
hyperiodothyroninaemia of neonates, its significance for thermogenesis.danowski et al. (1951) were probably the first who showed an increase of pbi in infants shortly after birth. later a number of investigators presented further evidence on the increased thyroid gland function in newborns. this condition named "neonatal thyroid hyperactivity" [fisher and oddie, 1964] was described also in animals. the available data indicate that all the newborn mammals till now studied, independently of the maturation stage of development they reach at birth, display some feature ...19883257052
influence of epidermal growth factor on intestinal function in the rat: comparison of systemic infusion versus luminal perfusion.epidermal growth factor may be a trophic substance for the small intestine. previous studies had not evaluated intestinal absorption after long-term continuous administration of epidermal growth factor or compared luminal perfusion with systemic infusion. epidermal growth factor (150 micrograms/kg/day) was continuously infused either systemically or luminally for 14 days into young adult fisher strain rats. luminal studies were performed by creating 10 cm thiry-vella loops. at the conclusion of ...19883257656
enhancement of aldehyde dehydrogenase activity in human and rat hepatocyte cultures by 3-methylcholanthrene.aldehyde dehydrogenase was measured in primary cultures of hepatocytes obtained with a two-step collagenase perfusion either from human hepatic tissue or from livers of fisher rats. basal enzyme activity declines gradually as a function of time in culture, but remains at all times higher when measured with propionaldehyde and nad (p/nad) than with benzaldehyde and nadp (b/nadp). treatment of the cultures with 2 microm of 3-methylcholanthrene for four days significantly increased the b-nadp activ ...19863267450
five vs. ten days of therapy for acute otitis a double blind study 175 patients with acute otitis media were randomized into 2 treatment groups: 10 days of therapy with cefaclor or 5 days of therapy followed by 5 days of placebo. the dosage of cefaclor was 40 mg/kg/day administered orally in equally divided doses at 12-hour intervals. tympanocentesis before treatment yielded specimens that contained streptococcus pneumoniae or haemophilus influenzae or both in 55% of specimens. branhamella catarrhalis was isolated from 21% of specimens. ...19883277153
inhibition of dunning tumor growth by melatonin.injections of the pineal hormone melatonin reduced growth and increased doubling time of the r3327h dunning prostatic adenocarcinoma in the copenhagen x fisher rat. this occurred even though testosterone levels (50% of normal) were ostensibly high enough to maintain normal tumor growth. melatonin may act directly upon the conversion of testosterone to dihydrotestosterone to inhibit tumor growth. alternatively, a more complicated mechanism may be involved since studies on mcf breast cancer tissue ...19883283381
wheezing in infants: the response to epinephrine.there is significant controversy about the role of bronchodilator therapy for wheezing in infants. a double-blind, randomized trial of subcutaneous epinephrine upsilon normal saline was conducted in children less than 24 months of age evaluated at yale-new haven hospital. respiratory assessments using a newly developed respiratory distress assessment instrument were made at baseline and 15 minutes after each of two injections. relief of respiratory distress was assessed using strict a priori cri ...19873295741
expression and characterization of transforming growth factor alpha precursor protein in transfected mammalian cells.analysis of a cdna clone derived from retrovirus-transformed rat fibroblasts has recently suggested that the mature 50-amino-acid form of transforming growth factor alpha (tgf alpha) is derived from a 159-amino-acid transmembrane precursor by proteolytic cleavage. to understand the processing of the tgf alpha precursor molecule in more detail, we have expressed this protein in baby hamster kidney (bhk) fibroblasts under control of the metal-ion-inducible metallothionein promoter and characterize ...19873299049
laser-assisted end-to-side anastomosis.the milliwatt co2 laser was used to anastomose rat carotid arteries in an end-to-side fashion, and this technique was compared to the standard suture technique. vessels were studied at two to six weeks. all anastomosis were patient and overall, there was a 78.6 percent rate of aneurysm formation in the laser cohort, compared with 7.1 percent with suture (p less than 0.001 fisher exact). vessels and aneurysm histology were similar to those previously reported for the end-to-end technique.19873309274
reimplantation injury after lung transplantation in a rat model.distant procurement of organs for transplantation requires satisfactory preservation to reduce injury during ischemia and the initial phase of reperfusion. we have studied the mechanism of reimplantation injury after unilateral lung transplantation in isogeneic fisher rats. the heart and lungs were removed en bloc from donor rats and preserved at 4 degrees c. after 5 hours the left lung was transplanted into a recipient. radiographic and histologic evidence of pulmonary edema in the transplanted ...19873312534
postirradiation alterations of neuronal chromatin structure.previous work from our laboratory suggested that neuronal chromatin structure may be altered immediately after exposure to ionizing radiation. in the present study, whole brains of 4-month-old male fisher 344 rats were irradiated with a dose of 25 gy. the kinetics of restoring the chromatin structure to its unirradiated state was investigated in rat cerebellar neurons using three different approaches: (1) measurement of changes in the dna superhelical structure by the fluorescent halo assay, (2) ...19883353510
features of the in vitro established rat large granular lymphocyte leukaemia rnk-16.a large granular lymphocyte (lgl) leukaemia cell line from the fisher/f344 rat strain called rnk-16 has been established in vitro, maintaining the same surface markers as tumour cell growing in vivo. the tumour has also maintained its specificity pattern and cytotoxic reactivity and serves as a suitable source of natural killer (nk)-like effector cells in vitro. the cells show no evidence of dependency on, or production of, interleukin 2 or interferons, nor is the cytotoxic capacity influenced b ...19883353687
comparative toxicity of halothane, isoflurane, hypoxia, and phenobarbital induction in monolayer cultures of rat hepatocytes.hypoxia, phenobarbital induction, and halothane anesthesia have been implicated in the pathogenesis of hepatotoxicity in the rat model. however, a controversy exists over the role of halothane in liver injury; does it act by reducing hepatic blood flow, thereby inducing hypoxia, or do its metabolites initiate the injury? these variables are difficult to separate during in vivo halothane exposure. in the present experiments, effects of halothane on hepatic perfusion were eliminated by exposing co ...19883354886
blood-brain barrier transport of choline is reduced in the aged age-related impairment in choline transport across the blood-brain barrier (bbb) may contribute to the cholinergic mechanisms of geriatric memory dysfunction. to test this hypothesis, the brain choline uptake in male fisher 344 rats at 2, 18 and 24 months of age was studied using the oldendorf technique. the vmax of choline transport in the 24-month-old rats (0.05 +/- 0.04 nmol/min/g) was significantly lower than that in the 2-month-old rat (2.5 +/- 1.0 nmol/min/g) (p less than 0.05). the km ...19883359216
molecular cloning of cdna corresponding to mrna species whose steady state levels in the thyroid are enhanced by thyrotropin. homology of one of these sequences with ferritin h.a lambda gt11 cdna library was constructed using poly(a)+ mrna from thyrotropin (tsh)-stimulated fisher rat thyroid (frtl5) cells. the library was screened for nonthyroglobulin cdna sequences by differential plaque filter hybridization using single-stranded cdna probes synthesized from mrna prepared from quiescent and tsh-stimulated frtl5 cells. thyroglobulin cdna-containing recombinants in the library were avoided by prehybridizing the tsh probe to excess thyroglobulin cdna. of 48,000 clones sc ...19883366767
photoperiodic sensitivity of prepubertal female fisher 344 rats.the laboratory rat is thought to be a poor model for study of the photoperiodic control of reproduction; however, this has only been investigated in a few rat strains. the purpose of the present investigation was to determine if the neuroendocrine-reproductive system of the fisher 344 (f344) rat, an inbred strain, is sensitive to light deprivation. all treatments were performed on 28-day-old female f344 rats and the animals maintained for 8 weeks in a 14:10 light:dark cycle. blinding resulted in ...19883367261
effects of nicotine on the fertility, cytology and life span of male rats.contrary to an earlier opinion that nicotine has no effect on the fertility of male animals or humans, the present experimental study using male inbred fisher rats demonstrates that the reproductive capacity of the animals is greatly reduced when injected with nicotine, and that the effect is much greater in male than in female fisher rats similarly injected with nicotine. this is in accord with some earlier histological and morphological studies which have shown that female rodents have a great ...19883369287
[changes in capillary permeability in experimental brain tumor].it is still controversial whether edema fluid around a malignant brain tumor is derived from capillaries inside the tumor itself or not only from tumor vessels but also from peritumoral tissue. the purpose of this study is to clarify the region where the capillary permeability is increased using double autoradiographic method. a suspension of 1 x 10(4) rat glioma cells (rg-12) was stereotactically implanted into the right basal ganglia of c-d fisher rats. in this model, all animals are dead 20 + ...19883370167
ventricular fibrillation during right coronary arteriography with ioxaglate, iohexol and iopamidol in dogs.radiograph contrast media (cm) are known to produce myocardial disturbances during cardiac angiography. the most severe electrical disturbance is ventricular fibrillation (vf). previous studies using prolonged right coronary exposures have demonstrated a higher incidence of vf with dilute low sodium cm than with dilute cm containing more physiologic levels of sodium. in this study the incidence of vf was examined for more conventional concentrations of iopamidol, iohexol and ioxaglate and for so ...19883372179
production and characterization of monoclonal antibodies to the protective antigen component of bacillus anthracis toxin.thirty-six monoclonal antibodies to the protective antigen protein of bacillus anthracis exotoxin have been characterized for affinity, antibody subtype, competitive binding to antigenic regions, and ability to neutralize lethal and edema toxin activities. at least 23 antigenic regions were detected on protective antigen by a blocking, enzyme-linked immunosorbent assay. two clones, 3b6 and 14b7, competed for a single antigenic region and neutralized the activity of both the lethal toxin in vivo ...19883384478
generation of anti-ssdna antibodies by persistent immunization of mice with sheep erythrocytes.immunization of (dba/2) mice with sheep erythrocytes (1 x 10(8) red cells, once weekly for 2 months) elicited anti-sheep erythrocyte antibodies, a part of which combined with ssdna. by contrast, immunization with rat (fisher) erythrocytes (1 x 10(8) red cells, once weekly for 2 weeks) did not elicit antibodies cross-reactive with ssdna. the antibody response (igm and igg) to sheep erythrocytes rose sharply and subsequently tapered off (usually within the first 2 weeks). the level of igg antibodi ...19883391634
focal brain ischemia in the rat: methods for reproducible neocortical infarction using tandem occlusion of the distal middle cerebral and ipsilateral common carotid arteries.this article describes a 3-year experience with focal neocortical ischemia in three rat strains. multiple groups of adult wistar (n = 50), fisher 344 (n = 31), and spontaneously hypertensive (n = 72) rats were subjected to permanent occlusion of the distal middle cerebral (mca) and ipsilateral common carotid arteries (cca). twenty-four hours later the animals were killed, and frozen brain sections were stained with hematoxylin and eosin to demarcate infarcted tissue. the infarct volume for each ...19883392112
growth of dunning transplantable prostate adenocarcinomas in rats fed diets with various fat contents.the effects of dietary fat concentration (5 vs. 20% corn oil, 0.5 vs. 20% corn oil and fat-free vs. 20% corn oil) on the growth of the dunning r3327-h (hormone-sensitive, well-differentiated, slow-growing) and r3327-150 (hormone-insensitive, anaplastic, rapidly growing) transplantable prostate tumor sublines were studied in copenhagen x fisher f1 male rats. rats fed 5 vs. 20% corn oil or 0.5 vs. 20% corn oil showed no differences in either r3327-h or r3327-150 tumor growth. fat-free diets had no ...19883392600
visualization and quantification of transplanted dunning prostate tumors in rats using magnetic resonance imaging.quantitative magnetic resonance imaging (mri) has been used for the in vivo size determination of subcutaneously implanted dunning r3327-h tumors in male copenhagen-fisher rats (n = 18). images have been recorded using a multislice spin-echo sequence se(1000/36) with a resolution of 0.2 x 0.2 mm2 in the imaging plane and a slice thickness of 2 mm. the reliability in the mri size determination was of the order of 10%. the mri results were compared with caliper measurements. five months after tumo ...19883393495
metabolism of pyrilamine maleate in fischer 344 rats, part i: activity excretion profiles.male and female fisher 344 rats (12 per group) were dosed by gavage with either 2 or 10 mg (based on the free amine) pyrilamine maleate containing about 12 and 6 muci 14c-pyrilamine maleate, respectively, to determine excretion of the activity as a function of dose and sex with time. urine and feces were collected at timed intervals through 144 h. most of the dose (about 70%) was eliminated within 48 h through the urine and feces, but only about 80% of the total dose was recovered during the exp ...19873431093
enhanced expression of harvey-ras oncogene in fanft-induced transitional cell carcinoma.the level of mrna expression and dna structure of c-h-ras were examined in a transitional cell carcinoma (tcc) induced in c3h/he mice and fisher rats by n-(4,5-nitro-2-furylthiazolyl)formamide (fanft) and carried as serially transplanted tumors. neither amplification nor gross rearrangements were detected by southern analysis in the fanft tumor. increased expression of c-h-ras was detected by northern and in situ hybridization in both the fanft-induced mouse and rat tcc, as compared to normal ur ...19873433577
molecular cloning and sequence analysis of the cdna for small proteoglycan ii of bovine bone.the cdna for the full-length core protein of the small chondroitin sulphate proteoglycan ii of bovine bone was cloned and sequenced. a 1.3 kb clone (lambda pg28) was identified by plaque hybridization with a previously isolated 1.0 kb proteoglycan cdna clone (lambda pg20), positively identified previously by polyclonal and monoclonal antibody reactivity and by hybrid-selected translation in vitro [day, ramis, fisher, gehron robey, termine & young (1986) nucleic acids res. 14, 9861-9876]. the cdn ...19873435485
effect of lithium on circadian activity of ache (ec in the reticular formation of mouse brain stem under ld 12:12.the investigation was carried out on male mice 6-8 weeks old. control animals were fed standard chow without lithium, while the experimental ones received on additional 10 mmol licl/kg of chow. after 30 days feeding, 7 mice at a time were killed every 4h. the activity of free ache was assessed in the brain stem reticular formation according to the method of ellman. the results were statistically analyzed using student's t-test and fisher test. parameters of periodic function were then applied to ...19873446450
comparative ototoxicity of gentamicin in the guinea pig and two strains of rats.gentamicin ototoxicity and nephrotoxicity were compared in two strains of rats, sprague-dawley and fisher-344, and in the hartley albino guinea pig. treatment groups consisting of 8 male rats of each strain and four male guinea pigs were dosed subcutaneously for 14 days with either 80 or 100 mg/kg of gentamicin sulfate in saline. brainstem auditory evoked response (baer) thresholds were recorded from each animal in each group on day 11 post-administration. blood urea-nitrogen and serum creatinin ...19873446672
changes in the hepatic perfusion index during the growth and development of experimental hepatic micrometastases.micrometastases were induced in fisher rats using an intraportal inoculation of 0.2 ml of 8 x 10(7) walker carcinosarcoma cells. a control group received normal saline. the hepatic perfusion index (hpi) was measured during the growth and development of micrometastases. the hpi at 4 days (0.51 +/- 0.008) and at 6 days (0.65 +/- 0.16) was significantly raised when compared to controls (0.31 +/- 0.07) and at 2 days after inoculation (0.31 +/- 0.06). hepatic artery flow did not change throughout the ...19873449796
effect of retinoids on the growth of squamous cell carcinoma of the palate in rats.the in vivo effects of retinoids on head and neck tumors were studied using fisher 344 rats with squamous cell carcinoma implanted in the palate. retinoids were given orally to rats, starting 2 weeks after tumor implantation. the animals were killed at 4 weeks, and the tumor volumes and body weights were measured. the animals had a greater response to all-trans-retinoic acid (58 per cent) and 13-cis-retinoic acid (60 per cent) than to all-trans-retinol (20 per cent) and 13-cis-retinal (30 per ce ...19863456728
radiofrequency hyperthermia with successive monitoring of its effects on tumors using nmr spectroscopy.radiofrequency (rf) hyperthermia was generated on rat glioma inoculated s.c. in cd fisher rats by applying the rf pulse using the surface coil in the nmr spectrometer, and the effect was monitored successively in the same spectrometer by measuring 31p nmr spectra and 1h nmr images. in the 31p nmr spectrum at the preirradiation stage, nucleoside triphosphate peaks and a phosphomonoester peak were high and a pi peak was low. after a rf pulse at a power of 5 w was applied continuously for 60 min, t ...19863464955
stimulatory effect of interleukin-1 upon hepatic metabolism.the liver plays an important role in the acute-phase response to sepsis and injury, and host survival often depends upon an adequate hepatic response. many of the metabolic sequelae to sepsis and injury are mediated by interleukin-1. this study was undertaken to investigate the impact of interleukin-1 upon hepatic metabolism and whether this mediator acted directly upon the liver. interleukin-1 (5 rabbit pyrogen dose units) was administered to male fisher f344 rats (175 to 200 g), and hepatocyte ...19863486338
outbreak of candida parapsilosis endophthalmitis after cataract extraction and intraocular lens implantation.between november 1983 and january 1984, 13 cases of candida parapsilosis endophthalmitis occurred in florida, georgia, and tennessee in patients who had had an intraocular lens implantation (ioli) or cataract extraction with an ioli. this outbreak followed the introduction in july 1983 of a new brand of balanced salt solution (bss) used as an intraoperative ophthalmic irrigation solution. this product was subsequently recalled because of intrinsic fungal contamination. a retrospective cohort stu ...19863490490
development of an experimental animal model for reactive arthritis induced by yersinia enterocolitica infection.we attempted to induce experimental arthritis in rats by systematically testing the effect of yersinia infections in five strains of rats, using the intragastric, intraperitoneal, and intravenous routes of inoculation. we observed that lewis rats which were given 10(4) to 10(5) yersinia enterocolitica wa organisms via the intravenous route consistently developed arthritis. the arthritis was most severe at 3 weeks and subsided at 6 weeks. no arthritis was observed when this bacterial strain was a ...19873493220
co-purification of transforming growth factor beta-like activity with pth-like and bone-resorbing activities from a tumor associated with humoral hypercalcemia of malignancy.humoral hypercalcemia of malignancy (hhm) is caused by a circulating bone-resorbing factor or factors. suggestions as to the nature of this factor include pth-like proteins, transforming growth factors, and bone-resorbing factors distinct from either of the first two classes of polypeptides. we investigated the occurrence of these three activities in a highly purified extract of the h-500 leydig cell tumor which causes hhm when implanted into fisher rats. pth-like adenylate cyclase-stimulating a ...19873494596
direct stimulation of the adrenal cortex by interleukin-1.the impact of human recombinant beta-interleukin-1 (il-1) on adrenocortical stimulation was investigated. this study asked three questions: does il-1 increase the corticosterone levels of rat serum? is there a direct effect on the adrenal cortex? what is the mechanism of this effect? the intraperitoneal injection of il-1 (70 micrograms) in anesthetized male fisher rats resulted in elevated corticosterone levels at 30 minutes and reached a maximum at 180 minutes (94 +/- 12 versus 34 +/- 4 microgr ...19873497458
survival of sylvatic trichinella spiralis isolates in frozen tissue and processed meat products.the ability of trichinella spiralis larvae to survive at subfreezing temperatures encysted in the musculature of wild carnivorous mammals was assessed by evaluating motility and infectivity (to rodents) of trichinae at various intervals after storage in frozen skeletal muscle. fifty to 60% of the larvae in grizzly bear meat were alive after storage for 27 months at -6.5 to -20 c, and 30% to 50% were still alive at 34 months. however, none survived for 38 months, on the basis of infectivity in mi ...19863505925
in vitro estimation of acoustic parameters of the liver and correlations with histology.freshly excised human liver specimens (77) were investigated echographically and histologically. the echography was concerned with the acoustic parameters: speed of sound, impedance, several attenuation parameters, and the texture parameters: reflectivity and the signal to noise ratio. it was found that the speed and impedance, the attenuation parameters, and the texture parameters did not correlate with each other. the major correlation between histologic parameters was found for the focal coll ...19863515717
some parameters of conditioned immunosuppression: species difference and cs-us delay.three experiments were conducted in which an illness-inducing immunosuppressant, cyclophosphamide (an unconditioned stimulus, us) was associated with a previously presented saccharin solution conditioned stimulus (cs). in each experiment, reexposure to the cs produced a conditioned suppression of the plaque-forming-cell response in the experimental groups. experiment i demonstrated this result with fisher 344 rats. experiment ii replicated the effect with balb/c mice. in experiment iii condition ...19863520612
different interactions used by cro repressor in specific and nonspecific dna binding.the mode of interaction of cro repressor with specific and nonspecific sites on dna was explored by chemical modification and protection of lysine and tyrosine residues. cro has 8 lysines. in the presence of dna, lysines 32 and 56 are fully protected and lysines 21, 62, and 63 are partially protected from alkylation. however, the terminal amino group and lysines 8, 18, and 39 are not protected. location of the protected and unprotected lysines on the three-dimensional cro structure defines a dna ...19863522575
genitalia in children's drawings: an association with sexual abuse.many sexual abuse victims have been observed to draw genitalia on human figures. to test the hypothesis that sexually abused children draw genitalia on human figures more often than do nonabused children, drawings from 57 children, 3 through 7 years of age, who were referred to child protective services as alleged sexual abuse victims, were compared with drawings from an age-, sex-, race-, and socioeconomically matched group of 55 nonabused children receiving well-child care in medical settings. ...19873540833
skin graft and kidney transplantation in rats. relation between circulatory condition of the transplanted organ and determine the relationship between histocompatibility and circulation in the transplanted graft, which are the major factors influencing graft survival, the survival of the skin grafts and kidney transplants were compared between strong (aci to lewis) and weak (fisher to lewis) histocompatible strains, because rats given skin graft receive poor blood supply from a recipient, and the rats given kidney transplants receive immediate blood supply after transplant. in the group with a weak histoco ...19863547526
the effect of nicotine and alcohol on the fertility and life span of rats. a cytological analysis.inbred fisher and buffalo rats were exposed to nicotine and alcohol. fertility was greatly reduced in both strains with nicotine treatments being much more deleterious than alcohol use. fisher rats tolerated both toxins better than buffalo rats. both strains became 'extinct' after one generation of fetal and postnatal exposure to nicotine, but alcohol-ingesting fisher rats had 3 or more generations of offspring. the total reproductive period was significantly shortened in both strains under the ...19873548201
capillary tortuosity and degree of contraction or extension of skeletal muscles.the effect of muscle contraction, and extension, on capillary anisotropy was investigated in rat m. soleus fixed by vascular perfusion at sarcomere lengths ranging from 1.62 micron (tetanizing stimulation of sciatic nerve) to 2.85 micron (ankle joint maximally flexed. capillary length density and tortuosity were estimated by morphometry using two sets of sections (0 and 90 degrees to the fiber axis). capillary orientation distribution was evaluated from a series of sections taken at 0 to 90 degr ...19873550394
theoretical analysis of single-round transcription experiments on trp leader region.a kinetic model is proposed to reproduce the time courses of the concentration change in paused leader rna, terminated leader rna, and readthrough rna in the single-round transcription experiments on trp leader region of escherichia coli and its mutants, l132, l75, and l75l135 (winkler, m. e., and c. yanofsky, 1981, biochemistry, 20:3738-3744; fisher, r., and c. yanofsky, 1983, j. biol. chem., 258:9208-9212). this model fits the experimental results well and also captures the essential aspects o ...19873552070
degeneration of hippocampal fibers and spatial memory deficit in the aged rat.old and young fisher 344 rats were compared for their ability to learn a delayed alternation task. the old animals displayed significant impairment of alternation learning, and were slower than the young animals. the brains of these animals were examined using a silver degeneration stain, and among old rats there was conspicuous degeneration. the greatest density of degenerating fibers was seen in the hippocampus and in anatomically related tracts, but there was substantial fiber staining in the ...19873561664
effect of aging on pancreatic lipolytic enzymes.pancreatic lipolytic enzyme activities and plasma lipids were measured in three age groups of female fisher 344 rats (3 months [young], 12 months [adult], and 27 months [old]) in order to evaluate age-related changes and a possible correlation of these parameters. cholesterol esterase activity was measured in pancreas homogenate, while the lipase activity was further fractionated into heparin-sepharose unretained (lipase i) and retained (lipase ii) fractions. in analogy to the lipolytic enzymes ...19863562438
heat-stress proteins of rat lung endothelial and mammary adenocarcinoma cells.a rat mammary adenocarcinoma cell clone, mtc, and a rat lung endothelial cell clone, rle cl.4, both syngeneic to the fisher 344 rat, were compared for proteins synthesized at 37 degrees c and after a 1-h, 42 degrees c heat dose. the heat stress-induced or -enhanced synthesis of a series of molecular mass groups and isoelectric point species (isomers) was observed in both equilibrium and nonequilibrium two-dimensional gel electrophoresis. tumor and endothelial cell heat-stress proteins (hsp) were ...19873562792
ligand blotting with 125i-fluoresceinamine-heparin.a highly sensitive method for ligand blotting with heparin has been developed. this ligand-blotting method is successful largely due to the ability to prepare heparin derivatives of high radiospecific activity. heparin was modified with fluoresceinamine according to the method of c.g. glabe, p.k. harty, and s.d. rosen [1983) anal. biochem. 130, 287-294), and this fluoresceinamine-derivatized heparin can be radioiodinated to a specific activity of 100,000 cmp/ng of uronic acid. this is a 500-fold ...19873565746
laboratory proficiency test results on use of selective media for isolating pseudomonas cepacia from simulated sputum specimens of patients with cystic fibrosis.pseudomonas cepacia colonization of or infection in patients with cystic fibrosis (cf) has been associated with increased morbidity and premature death. however, current data on national incidence may be biased because of interlaboratory differences in the methods of culturing sputa of patients with cf. we conducted three tests to evaluate the proficiency of microbiology laboratories at cf centers in identifying and isolating p. cepacia and to assess the value of using selective media for p. cep ...19873571451
growth of 9-l and rt-9 gliosarcoma in female rats.published studies of 9-l and rt-9 gliosarcoma have been done only in male rats and it is widely assumed that these tumors do not grow in female rats. in this study 9-l and rt-9 gliosarcoma was inoculated into the brains of male and female fisher cd rats. the tumors grew with essentially the same incidence and survival time in both sexes, confirming that female rats can be used as well as male rats in experiments using rt-9 and 9-l gliosarcomas.19873598624
effects of dichloroacetate in spinal stroke in the rabbit.high levels of brain lactate may contribute to cellular death and dysfunction in acute cerebral ischemia. although sodium dichloroacetate (dca) has been shown to lower brain lactate in incomplete cerebral ischemia, functional outcome has not been assessed with dca. we examined the effects of dca treatment on functional neurologic outcome using a previously developed model for "spinal stroke" in the rabbit. thirty male new zealand white rabbits weighing 1.3-2.8 kg were studied. after anesthesia w ...19873613862
initiating activity in a two-stage mouse skin model of nine mutagenic pyrolysates of amino acids, soybean globulin and proteinaceous food.trp-p-1, trp-p-2, mea alpha c, a alpha c, glu-p-1, glu-p-2, lys-p-1, iq and phe-p-1 were tested for tumour initiating activity in a two-stage skin carcinogenesis model using 12-o-tetradecanoylphorbol-13-acetate (tpa) as the promoter. the total initiating doses were 20 mg for trp-p-1, trp-p-2, glu-p-1 and glu-p-2, 40 mg for mea alpha c and a alpha c, 5 mg for lys-p-1, 7.5 mg for iq and 100 mg for phe-p-1. 7,12-dimethylbenz[a]anthracene was used as a positive control compound at a total dose of 10 ...19873621463
the effect of indomethacin on burn-induced immunosuppression.recent interest in the role of prostaglandin inhibitors as immunomodulators following major injury prompted us to study the effect of indomethacin on burn-induced immunosuppression in rats as measured by the delayed-type hypersensitivity (dth) skin test response, ability to contain an intradermal bacterial challenge (10(8) staphylococcus aureus 502a injected intradermally), and overall survival from spontaneous burn wound sepsis. fifty male sprague-dawley rats sensitized to keyhole limpet hemocy ...19873626543
strain differences in circadian drinking behaviors of ethanol and water in rats.voluntary drinking responses to an alternating three-bottle, two-choice paradigm to tap water or to 5% ethanol were measured in adult male fisher (fshr; n = 14) and spontaneously hypertensive (sp; n = 16) rats for 6-9 days. all animals were singly caged and housed separately in isolation chambers. the animals received light from 0600 to 1800 (cst) daily (ld 12:12) or remained in constant darkness (dd) at room temperature (23 degrees c). food was freely available. water and ethanol bottles were c ...19873628356
bha (2(3)-tert-butyl-4-hydroxyanisole)-mediated modulation of acetaminophen phase ii metabolism in vivo in fisher 344 male fisher 344 rats (190-220 g), were given an intravenous dose (10 mg/rat) of bha. pretreated and control rats received an intravenous dose of [g-3h] acetaminophen (25 mg/rat). bile was collected prior to dosing and for 5-6 hours after dosing at varying time intervals. separate aliquots of 0.2 ml were incubated with beta-glucuronidase and sulfatase, respectively. these incubation mixtures were then extracted and analyzed by reverse phase hplc. in all cases control animals showed a greate ...19873628963
critical role of gonadal hormones on the genotoxic activity of the hepatocarcinogen dl-zami 1305.the dna damaging capacity of the sex-dependent hepatocarcinogen beta-blocker dl-1-(2-nitro-3-methyl-phenoxy)-3-tert-butylamino-propan-2-ol (dl-zami 1305) was evaluated in different sex hormonal conditions. a single injection of dl-zami 1305 causes dna damage in the liver of the female but not the male wistar rat. when the hormonal environment of the female rat is converted to 'male type' by ovariectomy and 1 week of treatment with testosterone, dna damage by dl-zami 1305 is completely abolished. ...19873652027
lens protein composition, glycation and high molecular weight aggregation in aging rats.because of minimal or no turnover, lens proteins are subjected to substantial post-translational modifications which in turn disrupt lens architecture and change the optical properties leading to senile cataract formation. progressive glycation is believed to have the potential to initiate the changes that are conducive to lens opacification. fisher 344 rats were systematically followed from juvenile to older and aged phases of their life to study the relationship between lens glycation and high ...19873654142
gentamicin-induced loss of basolateral surface area of rat proximal convoluted tubules.this study was performed to find the quantitative relationship between basolateral surface alterations of proximal convoluted tubules (pct) and renal failure in 26 fisher 344 rats receiving 20 mg/kg of gentamicin twice daily up to 28 days. they were sacrificed on day 14 (renal failure, 13 rats) and day 28 (recovering, 13 rats). control rats were used for morphologic studies (19) and serum creatinine studies (6 of 19). pcts (14th and 28th day) were graded as to severity with transmission electron ...19873669615
genetic variability in response to dietary calcium.supplemental dietary calcium has been shown to reduce blood pressure in spontaneously hypertensive rats while restricted calcium diets cause an elevation in blood pressure. this latter nutrient effect has been enhanced by modest sodium restriction and is associated with a reduction in serum ionized calcium concentration. to determine whether alterations of dietary calcium and sodium have a similar influence on blood pressure in genetically normotensive rats, fisher 344, wistar furth, and aci rat ...19873669918
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