correlation between liver iron content and magnetic resonance imaging in rats.currently, serum ferritin concentration is the best noninvasive estimator of liver iron content. this study investigated the ability of magnetic resonance imaging to determine hepatic iron concentration. fisher rats were treated with either parenteral iron to increase levels of phlebotomy to lower them and achieved a wide range of liver iron concentrations. rats were imaged using a clinical whole body scanner at 1.5 tesla with a 15-cm helmholtz surface coil and a 23-cm field of view. the ratio o ...19892630845
[morphofunctional transformations of the blood vessels in the transplantation of syngeneic normal tissue and of a tumor].in 360 fisher rats dynamics of changes in blood vessels, the microcirculatory bed vessels included, have been investigated. an original model of the experiment at implantation of syngenic tissues of the fetus normal intestine and tumorous adenocarcinoma of the small intestine has been used. specific changes of the blood vessels have been revealed around and in the capsule of the implant, depending on their morphological reorganization. the blood vessels changes can serve as a prognostic sign of ...19892631644
stress ulcer susceptibility and depression in wistar kyoto (wky) a series of studies, wistar kyoto (wky) normotensive rats were more susceptible to water-restraint-induced stress ulcer as compared to spontaneously hypertensive rats (shr) fisher-344 (f344) and wistar rats. in these same studies, wky rats were also deficient in several behavioral tasks. the four strains were observed in the open-field test of emotionality and wkys were judged more emotional. in a study on "learned helplessness" wkys were more deficient in the acquisition of a shuttlebox esca ...19892634265
the neutrophil-activating protein (nap-1) is also chemotactic for t lymphocytes.t lymphocyte chemotactic factor (tcf) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. the amino-terminal amino acid sequence of the purified tcf showed identity with neutrophil-activating protein (nap-1). both tcf and recombinant nap-1 (rnap-1) were chemotactic for neutrophils and t lymphocytes in vitro supporting the identity of tcf with nap-1. injection of rnap-1 into lymphati ...19892648569
cryopreservation does not alter antigenic expression of aortic allografts.cryopreserved aortic homografts are reportedly viable, but no cross-matching or immunosuppression is utilized. alterations of the antigenic expression by cryo-preservation must be assumed. we designed a protocol to test this premise. fisher 344 rats served as recipients in all cases. lewis rats, a mildly disparate strain, were utilized as donors. four cohorts of animals were utilized. group i (n = 11) served as a "first set" control. all animals received a syngeneic skin graft. after 28 days an ...19892659897
[neurologic involvement in campylobacter infections. 5 cases].campylobacter are a newly recognized class of human infectious agents. campylobacter fetus subspecies fetus is a cause of sepsis in immunocompromized hosts. secondary neurological determinations, meningitis and meningoencephalitis appear to be rare. we report 2 cases, and 8 previously reported cases are reviewed. campylobacter jejuni appears to be a common bacterial pathogen causing a syndrome of enteritis. since 1982 it has been associated with guillain-barré syndrome (gbs). in one serological ...19892664975
length changes in dendritic networks of cerebellar purkinje cells of old rats after chronic ethanol treatment.dendritic networks of cerebellar purkinje neurons from aging ethanol-treated fisher 344 rats were analyzed for metric changes in terminal and internal segments of the networks. mean lengths of three categories of dendritic segments were determined. no significant metric changes in segment lengths were detectable immediately at the termination of 24 weeks of ethanol treatment, but significant changes were apparent after 8 weeks of recovery from ethanol treatment. return to a diet of standard labo ...19892665558
molecular biology of friend viral erythroleukemia.friend virus clearly provides an important model for understanding the molecular biology of cancer. moreover, the most important aspects of the erythroleukemia can be caused by a single sffv infection in the absence of any helper virus. the sffv env gene encodes a membrane glycoprotein, gp55. this glycoprotein, when expressed on erythroblast surfaces, causes a constitutive mitogenesis. however, sffv infections only rarely increase the cell's self-renewal capability or abrogate its commitment to ...19892684547
putidaredoxin competitively inhibits cytochrome b5-cytochrome p-450cam association: a proposed molecular model for a cytochrome p-450cam electron-transfer complex.cytochrome b5 has been genetically engineered to afford a fluorescent derivative capable of monitoring its association with cytochrome p-450cam from pseudomonas putida [stayton, p. s., fisher, m. t., & sligar, s. g. (1988) j. biol. chem. 263, 13544-13548]. in the mutant cytochrome b5, threonine is replaced by a cysteine at position 65 (t65c) and has been labeled with the environmentally sensitive fluorophore acrylodan. in this paper, the physiological p-450cam reductant putidaredoxin, an fe2s2.c ...19892690937
mechanisms involved in the pathogenesis of yersinia infections.yersinia enterocolitica and yersinia pseudotuberculosis are food-borne enterobacterial pathogens which may initiate rheumatoid diseases. by molecular genetic analysis of the pathogenicity of these species virulence gene loci could be identified on the chromosome and on a plasmid. plasmid-encoded proteins mediate cell adherence, phagocytosis resistance, survival in serum, cytotoxicity, and collagen binding. y. enterocolitica of serotype 0:8 is mouse-lethal and arthritogenic for lewis rats. mouse ...19892692128
augmentation of the resistance against escherichia coli by oral administration of a hot water extract of chlorella vulgaris in previous studies, we demonstrated that a hot water extract of chlorella vulgaris (cve) augmented the resistance against an intraperitoneal infection with escherichia coli by its intraperitoneal, intravenous or subcutaneous administration. the augmented resistance appeared to be attributable to the enhanced activity of polymorphonuclear leukocytes (pmn). in this study, the effect of oral administration of cve against escherichia coli infection was examined. male fisher rats (f344/ducrj) were a ...19892693376
the need for a search for a proximal pathogenic principle of human aids.some of the unexplained aspects of hiv/aids epidemiology, virology, and pathology (6, 13) may thus be due to a lack of knowledge of the basic virology of the virus complex and its pathological interaction with cells of the host. there may be no need to call upon anomalous immune responses, vicious transactivating activities, or new virological or biological principles if we can first do the basic virology of the disease-inducing viruses. while we do not want to propose that working out the virol ...19892702660
structural characterization of nitrimyoglobin.nitrimyoglobin was formed in greater than 94% yield by a simple reaction between excess nitrite and horse heart metmyoglobin at ph 5.5. this dark green pigment was shown by 1h nmr spectroscopy to be a single, pure product with a well defined tertiary structure that is highly similar to the starting myoglobin. electronic spin states parallel those of myoglobin, although the relaxation times differ. ligand binding reactions of nitrimyoglobin parallel those of normal myoglobin, but lead to a unique ...19892703482
new sensitizers for photodynamic therapy: controlled synthesis of purpurins and their effect on normal tissue.purpurins are a class of porphyrin derivative that have been shown to have good in vivo cytotoxicity to n-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (fanft) induced rat bladder tumors (ay-27) implanted into fisher 344 rats. the synthesis of purpurins from etioporphyrin i and coproporphyrin i proceeds in high yield and with a high degree of regioselectivity. product formation can be rationalized in terms of relief of steric strain about the periphery of the purpurin macrocycle. the effect of ther ...19892704035
[expression of cell surface transformation-associated antigens taas on activated oncogene-transfected rat fibroblast and antitumor immunity by hosts].the key point in this study was that the cell surface antigens in the events of transformation were analyzed using the same clonal-derived nontransformed cell and various oncogenes. wka rat fetus-derived fibroblast line, wfb, showed the strict non-transformant phenotypes. transfection of ejras and polyoma middle t oncogenes into wfb resulted in the acquisition of tumorigenicity in vitro and in vivo. the newly expressed cell-surface antigens on some transformants were analyzed using four monoclon ...19892705790
comparative studies of radiotracer citrates in oncological models--2. 153sm-citrate and 67ga-citrate.153sm-citrate solutions were prepared from enriched 152sm2o3 which had been irradiated at 10(12)n cm-2 s-1 in the university of alberta slowpoke reactor. 153sm was rapidly bound (93% in 2 h) by melanoma 2ab cells in tissue culture upon incubation in the presence of 153sm-citrate (1.9 nmol 10(6) cells). in vitro ultracentrifugation studies of 153sm-citrate solutions showed that colloid formation under incubation conditions could have been responsible in part for the uptake by cultured cells. low ...19892714996
age-related alterations in duodenal calcium transport rate in determine the age-related alterations in intestinal calcium transport under in vitro controlled conditions, we studied the calcium transport across duodenal segments of prepubertal (2 months), young adult (6 months) and aged (26 months) fisher 344 male rats using the ussing chamber technique. we also evaluated the effect of 10(-11) m 1,25-dihydroxycholecalciferol [1,25-(oh)2d3] in tissue bathing media. the calcium transport rate from mucosa-to-serosa (jms) and from serosa-to-mucosa (jsm) in 2 ...19892716368
changes in the hepatic perfusion index during the development of experimental hepatic tumours.a model of microscopic liver tumour has been developed in the fisher rat by intraportal injection of 1.6 x 10(7) walker 256 carcinosarcoma cells. rats were studied at 2, 4 and 6 days after the inoculation of live walker cells. a control group received dead walker cells. no tumour was visible in control groups at 2, 4 and 6 days after inoculation. similarly in rats injected with live cells no tumour was visible at 2 days after inoculation but at 4 and 6 days the percentage hepatic replacement was ...19892720322
membranous glomerulonephritis and the nephrotic syndrome in a patient with landry-guillain-barré-strohl syndrome.membranous glomerulonephritis and the nephrotic syndrome concurrent with the miller-fisher variant of the landry-guillain-barré-strohl syndrome (lgbs), acute post-infective polyneuritis, is reported in a 49-year-old man. the onset of heavy proteinuria coincided with the development of the neurological disturbance. while immunosuppressive therapy appeared to hasten improvement in the neurological disease, no such improvement occurred in the glomerulopathy.19892728436
purification of a human milk protein closely similar to tumor-secreted phosphoproteins and osteopontin.a wide variety of rodent and human tumor cells secrete antigenically related phosphoproteins with molecular weights (mr) of approximately 58,000 (hamster), 62,000 (rat, mouse), 67,000 (human) (senger, d.r. and perruzzi, c.a. (1985) cancer res. 45, 5818-5823). expression of these phosphoproteins is transformation-related; tumor cells produce at least 10-fold or more of this protein as compared to their normal or untransformed counterparts. n-terminal and internal sequences derived from the rat tu ...19892736258
the problem of our common mitochondrial has been suggested that the mitochondrial dna (mtdna) of all present-day human beings stems exclusively from one woman who lived about 200000 years ago in africa; examination of the problem by the mathematical theory of random walks supposedly renders alternatives very unlikely. however, a statistical argument first used by fisher indicates that this hypothesis is untenable, at least if the assumptions made by previous workers are accepted. all present-day mtdna might go back to one individua ...19892737664
biodistribution of misonidazole and 1,3-bis(2-chloroethyl)-1-nitrosourea (bcnu) in rats bearing unclamped and clamped 9l subcutaneous tumors.the biodistribution of misonidazole (miso) and 1,3bis(2-chloroethyl)-1-nitrosourea (bcnu) was studied using the subcutaneous (s.c.) 9l tumor model in male fisher 344 rats. a transient hypoxia in these tumors was created by clamping the blood supply to the tumor. reoxygenation occurred upon release of the clamp. the plasma and tumor concentrations of miso and bcnu were quantitated by high pressure liquid chromatography. when 12 mg/kg of bcnu was given i.p. without miso, the peak plasma concentrat ...19892745188
morphologic and hemodynamic comparison of tumor and healing normal tissue microvasculature.the purpose of this study was to compare microvascular morphometric and hemodynamic characteristics of a tumor and granulating normal tissue to develop quantitative data that could be used to predict microvascular characteristics which would be most likely associated with hypoxia. the dorsal flap window chamber of the fisher 344 rat was used to visualize the microvasculature of 10 granulating and 12 tumor (r3230 ac adenocarcinoma) tissues at 2 weeks following surgical implantation of the chamber ...19892745213
aging: effect on hepatic metabolism and transport of folate in the rat.effects of aging on hepatic folate metabolism and transport were assessed in male fisher 344 rats. total serum and hepatic folate levels were measured. hepatic folates were measured by high-performance liquid chromatography and by lactobacillus casei assay. transport of 5-methyltetrahydrofolate (5-ch3-h4pteglu) was measured in isolated hepatocytes. serum folate declined with aging; however, neither the total folate level nor the distribution of hepatic folate coenzymes was affected by the aging ...19892756923
treatment of experimental pseudomonas aeruginosa pneumonia with a human igm monoclonal antibody.a human igm monoclonal antibody (ma-1c1) to fisher immunotype 3 pseudomonas aeruginosa lipopolysaccharide antigen was evaluated for in vivo activity in a guinea pig model of experimental pneumonia. pharmacokinetics of ma-1c1 were compared in infected and noninfected animals. intravenous bolus infusion of ma-1c1, 1 mg/kg, resulted in peak serum antibody concentrations of 3.8 +/- 0.08 and 3.7 +/- 0.05 micrograms/ml in infected and noninfected animals, respectively. serum half-lives were 25 and 22 ...19892760499
changes with aging in the levels of amino acids in rat cns structural elements. ii. taurine and small neutral amino acids.taurine (tau) and the small neutral amino acids glycine (gly), serine (ser), threonine (thr), and alanine (ala) were measured in 53 brain areas of 3- and 29-month-old male fisher 344 rats. the ratio of highest to lowest level was 34 for tau, 9.1 for thr, 7.6 for gly and ser, and 6.5 for ala. the heterogeneity was found in numerous areas; for example, tau levels were more than 90 nmol/mg protein in 6 areas, and less than 20 nmol/mg protein in 10 areas. similar heterogeneity was found with the oth ...19892761675
expression of a human multidrug resistance gene in ovarian carcinomas.expression of the human mdr1 gene has been shown to confer the multidrug resistance (mdr) phenotype to sensitive cells. to investigate the possible contribution of the mdr phenotype to chemoresistance in ovarian carcinoma, we have analyzed mdr1 gene expression in fresh carcinoma specimens from 50 patients. fifteen received chemotherapy before surgery and were judged as poor responders. thirty-five patients did not receive any drug before surgery. control tissues were lymphocytes from 7 patients. ...19892766278
chloroform inhibition of 1,2-dimethylhydrazine-induced gastrointestinal tract tumors in the fisher 344 rat.the effect of chloroform (chcl3), administered at 0, 900, and 1800 mg/liter in the drinking water, on the carcinogenic potency of 1,2-dimethylhydrazine (dmh) was investigated. groups of 40 male fisher 344 rats were given one of the three drinking water solutions for 39 weeks following the subcutaneous injection of 200 mg/kg dmh, a known gastrointestinal (gi) tract carcinogen in this animal strain. when tumors from the gi tract were pooled there was a highly significant (p less than 0.001) decrea ...19892767360
establishment and characterization of an epithelial cell line from the rat submandibular epithelial cell line, rsmtx, has been established from the submandibular gland of weanling fisher 344 rats by treatment of explanted tissue clumps with 3-methylcholanthrene. these cells exhibit a polygonal shape on light microscopy and a polar appearance, with desmosomes, terminal bar-like structures, surface microvilli and cytoplasmic interdigitations, when examined by electron microscopy. the cells react positively with an antiserum to cytoskeletal keratin, and a commercial monoclonal antib ...19892769593
correlation of mdr1 gene expression with chemotherapy in neuroblastoma.forty-one neuroblastoma tumor specimens have been analyzed by northern and slot blot hybridization techniques with human mdr1 gene probes. only one of 15 (6%) tumors from patients who had not received chemotherapy exhibited high levels of mdr1 transcripts, while 11 of 26 (42%) treated tumors showed high levels of mdr1 expression (fisher exact test: p = .03). the results indicate that the level of mdr1 mrna expression is associated with previous chemotherapy, including drugs that select the multi ...19892778826
temporal actions of 16 alpha-hydroxyestrone in the rat: comparisons of lordosis dynamics with other estrogen metabolites and between sexes.16 alpha-hydroxyesterone (16ohe1), a metabolite of estradiol (e2) and precursor of estriol (e3), binds to the estrogen receptor (er) with low affinity (3% of e2), but is estrogenic in both in vitro and in vivo systems. this metabolite is able to bind in a non-dissociable manner to the er. we examined these properties in vivo by assessing the temporal dynamics of estrogen metabolite action in the rat brain, using lordosis score (ls) to manual stimulation as a serial bioassay of estrogen effect. m ...19892779234
interleukin-1-induced anorexia in the rat. influence of prostaglandins.the anorexia associated with acute and chronic inflammatory or infectious conditions is poorly understood. our objectives were to explore the anorexigenic effects of interleukin-1 (il-1) in the rat. recombinant human (rh) il-1 beta, murine (rm) il-1 alpha and to a lesser extent rhil-1 alpha significantly reduced food intake at greater than or equal to 4.0 micrograms/kg i.p. but not at lower doses, in young (200-250 g) meal-fed rats on chow diets. the anorexic effect appears to be mediated by pro ...19892786888
effect of magnesium deficiency on interleukin production by fisher rats: effect of interleukins on reduced in vitro lymphocyte responses to concanavalin a and lipopolysaccharide.the ability of rats fed a magnesium-deficient diet to produce interleukins (ils) and the effect of ils on in vitro lymphocyte mitogenesis have been studied in rats. lack of magnesium resulted in a lower number of plastic-adherent spleen cells and in a reduction of il-1 production. il-2 production was not significantly affected, indicating differential sensitivity of t cells to magnesium deficiency. the diminished mitogenic response of splenocytes to concanavalin a (con-a) was restored by the add ...19892788143
immortalization of rat spleen and thymus t cells by human t-cell leukemia virus type of thymus and spleen cells of fisher and lewis rats with lethally irradiated mt-2 cells harboring human t-cell leukemia virus type i (htlv-i) resulted in the establishment of lymphoid cell lines, firt-1, firs-1, lert-1, and lers-1, respectively. cells of these cell lines had rat t-cell characters as demonstrated by the positive reaction to monoclonal antibodies (mabs) to rat t cell antigens (thy 1 and pan t). they lacked surface immunoglobulins and strongly expressed rat interleuk ...19892788356
effect of trimethoprim/sulphamethoxazole and hyperbaric oxygen on experimental spiroplasma mirum encephalitis.this study was undertaken to determine the susceptibility of experimentally induced spiroplasma mirum infection in the rat to trimethoprim/sulfamethoxazole (tmp/smx) in combination with hyperbaric oxygen (hbo). one-day-old fisher 344 rats were intracerebrally inoculated with the gt-48 strain of s. mirum and were exposed to regimens employed combined antibiotic and hbo treatments. the exclusive use of tmp/smx produced a significant reduction in mortality (p less than 0.0001) and an absence of cli ...19892799063
subfornical organ lesions in rats abolish hyperdipsic effects of isoproterenol and serotonin.isoproterenol (300 micrograms/ml/kg) and serotonin (2 mg/ml/kg) given sc to rats (n = 27) caused significant drinking (fisher plsd, scheffe f test, dunnett t) in the 1 and 2 hours after injection. such drinking was completely prevented in rats later shown to have complete lesions of their subfornical organs (n = 7). in contrast a response not significantly different from the prelesion response was found in rats later given cortical lesions (n = 11) or other lesions which did not damage the subfo ...19892804709
1,2-dimethylhydrazine-induced carcinogenesis influenced by different colonic anastomoses in rats.dispensing subcutaneously 1,2-dimethylhydrazine, intestinal carcinogenesis was investigated in male fisher rats with different surgical colonic anastomoses, producing blind gut loops of isoperistaltic high (fecal stasis) and anisoperistaltic low fecal contact. one hundred and eight rats, except 1 rat of the control group, developed colonic neoplasms. in contrast to the control group, mainly in anastomotic areas and in isoperistaltic blind gut loops with intensive fecal contact huge adenocarcinom ...19892806346
characteristics of submucosal lymphoid tissue located in the proximal colon of the this study we have examined the morphology and steroid sensitivity of proximal colonic lymphoid tissue in the fisher 344 rat. a time course study was conducted in which groups of animals were injected subcutaneously with hydrocortisone sodium succinate (125 mg/kg body weight) and killed on days 0-4. thymus, jejunal and ileal peyer's patches and proximal colonic lymphoid tissue were excised, weighed and processed for histological analysis. the results showed that the maximum cytoreductive effe ...19892808123
morphometric analysis of the cell outlines of normal and polyomavirus-transformed fr 3t3 fibroblasts.a method is described for the analysis of cell shape, using an image analyzer connected to a computer to assess the cell outline. a series of parameters to assess the contribution of large cytoplasmic expansions to cell morphology and to cell spreading on a planar substratum were used to quantify the visual morphologic differences between normal (nontransformed; n.3t3) and polyomavirus-transformed (py.3t3) fisher rat 3t3 fibroblasts. the results show that the py.3t3 fibroblasts are more spherica ...19872822058
induction of a novel ca2+-dependent protease in preneoplastic and neoplastic liver tissues during rat liver carcinogenesis.the activity of a novel ca2+-dependent protease in preneoplastic and neoplastic tissues of male fisher 344 rat liver was examined during experimental liver carcinogenesis. this protease activity is significantly high in hyperplastic nodules and hepatocellular carcinomas which were induced by the method of solt and farber. taken together with our previous report on the promoter-specific induction of this protease, the results suggest the possible importance of this protease in the promotion stage ...19872824086
temperature-sensitive cellular mutant for expression of mrna from murine retrovirus.the cellular mutant b812 isolated from a fisher rat cell line shows temperature sensitivity of focus formation induced by various retroviruses such as recombinant murine retrovirus containing the middle t gene of polyomavirus (pymlv), kirsten murine sarcoma virus, moloney murine sarcoma virus, and recombinant murine retrovirus containing the src gene of rous sarcoma virus. b812 cells, however, show normal ability to proliferate and synthesize protein at the nonpermissive temperature, suggesting ...19882824838
herpes simplex virus-induced dutpase: target site for antiviral studies using mutants of hsv-1 (strain 17), which are defective in inducing the hsv-specific dutpase, fisher and preston concluded that since this enzyme was not essential for hsv replication, it would not be useful as a target site for the development of antiviral agents. in this study, we demonstrate that while these mutants do not induce a hsv-specific dutpase, they do not shut off cellular dutpase activity in infected cells and that the cellular dutpase can function in place of the hsv-in ...19882842952
cytomegalovirus pneumonia after bone marrow transplantation successfully treated with the combination of ganciclovir and high-dose intravenous immune assess the efficacy of the combination of the antiviral agent ganciclovir (9-1,3 dihydroxy-2-propoxymethylguanine) and high-dose intravenous immune globulin for treating cytomegalovirus interstitial pneumonitis after allogeneic bone marrow transplantation.19882847609
relationship between cellular adhesiveness and metastatic activity in polyomavirus-transformed fr3t3 rat cell lines.a series of polyomavirus-transformed rat cells with varying tumorigenic potential were tested for biophysical parameters possibly related to metastatic properties: adhesive capacity and strength of adhesion to different substrates (laminin, fibronectin and albumin), cell deformability and spreading. two groups of cell lines were defined according to their higher or lower adhesive capacity. adhesivity did not appear to be related to cell deformability and spreading. a weak correlation was suggest ...19882847989
a study of fine specificity of monoclonal antibodies to yeast iso-1-cytochrome monoclonal antibodies, prepared to yeast holo- or apo-iso-1-cytochrome c by the method of köhler and milstein (goding, j. w. (1983) monoclonal antibodies: principles and practice, academic press, orlando, fl) were characterized by cross-reaction with a panel of evolutionarily related cytochromes c, apocytochromes c, fragments and homologous and hybrid fragment complexes, inhibition, competitive inhibition, and complementation and fluorescence titration. the results have permitted us to ass ...19882848803
statistical analysis of morphometrically analyzed outlines of cells in culture. comparison of populations of normal and polyomavirus-transformed fr 3t3 fibroblasts.the correspondence analysis method was used to statistically characterize the morphologies of populations of normal (nontransformed; n.3t3) and polyomavirus-transformed (py.3t3) fisher rat 3t3 fibroblasts in culture, based on morphologic parameters calculated according to a previously described morphometric method. with this statistical method, each cell is considered as a vector in a space defined by an arrangement of the calculated morphologic parameters. the n.3t3 cells and the py.3t3 have tw ...19882849952
hepatocarcinogenic and promoting action of a choline-devoid diet in the rat.male fisher 344 rats were solely fed a choline-supplemented diet for 65 to 105 weeks or a choline-devoid diet for 24 to 102 weeks. hepatocellular carcinomas developed in the latter animals, beginning at 24 weeks. other groups of rats were given a single dose of 20 mg diethylnitrosamine/kg, 18 h after a partial hepatectomy and were fed, 4 weeks thereafter, either a choline-supplemented, or a choline-devoid diet for up to 48 weeks. in rats fed the choline-supplemented diet, the only relevant lesio ...19852859112
the capability for regulation of insulin secretion by somatostatin in purified pancreatic islet b cells during aging.pancreatic islet b cells from sprague-dawley and fisher 344 rats aged 3-27 months were separated from a and d cells by centrifugation over a linear percoll density gradient, and incubated in vitro with various concentrations of glucose and somatostatin. elevation of glucose concentration in the incubation medium from 2.6 to 16.7 mm provokes an insulin secretory response that is independent of rat donor age. inhibition of the insulin secretory response by somatostatin is independent of rat donor ...19862870220
the effects of reserpine and haloperidol on tyrosine hydroxylase activity in the brains of aged rats.many neurotransmitter systems appear to be altered with aging. the effects of aging on the regulation of tyrosine hydroxylase, the rate-limiting enzyme in the synthesis of catecholamines in the brain has been examined. the endogenous basal activity of tyrosine hydroxylase was lower in the hypothalamus of 24 month old fisher 344 rats than in the hypothalamus of 3 month old or 6 month old animals. there was no difference in the basal activity of tyrosine hydroxylase in the locus ceruleus, frontal ...19862874461
the dynamics of phase partition. a study of parameters affecting rat liver organelle partitioning in aqueous two-polymer phase systems.separation of subcellular organelles by two-phase partition is thought to reflect differential partition of the organelles between the two phases or between one of the phases and the interface. studies by fisher and colleagues [fisher & walter (1984) biochim. biophys. acta 801, 106-110] suggest that cell separation by phase partition is a dynamic process in which the partition changes with time. this is mainly due to association of the cells with sedimenting droplets of one phase in the bulk of ...19862874790
[neuromediator binding to receptors in the rat brain. the effect of chronic administration of ginkgo biloba extract].the present data confirm the results of others that post-synaptic receptor changes may contribute to the decline in brain cholinergic function in ageing and dementia. we have also shown that chronic oral treatment with an extract of gingko biloba increases the apparent muscarinic receptor population in the hippocampus of the aged fisher 344 rat. the possible effect on (3h) kainic acid binding to the kainate-excitatory amino acid site is also interesting because of the proposed association of neu ...19862878427
enzyme histochemistry of the rat nasal mucosa embedded in cold glycol methacrylate.the nasal passages are anatomically complex, and while there have been a number of descriptions of nasal structure in many species, there is very little information available on the distribution of enzymes in the nasal mucosa. in rodents, this delicate mucosa is the first site within the respiratory tract to be exposed during inhalation toxicology studies designed to assess human risks from such exposures. however, the nasal mucosa presents problems for histologic preparation because it is encas ...19872887103
immunosuppression by succinylacetone. ii. prevention of graft-vs-host disease.succinylacetone is a seven-carbon organic ketoacid that we have previously shown to inhibit tumor allograft rejection as well as the primary antibody response to sheep erythrocytes in rats. because it appeared to be such a potent immunosuppressive agent in our initial studies, we evaluated succinylacetone for its ability to block graft-vs-host disease (gvhd) in adult f1 rats injected with parental strain spleen cells. untreated ace x lewis f1 rats given lewis strain spleen cells died of gvhd, wi ...19872889780
lithium increases dynorphin a(1-8) and prodynorphin mrna levels in the basal ganglia of rats.the aim of this study was to understand the possible influence of the antimanic drug, lithium, and the neuroleptic, haloperidol, alone or in combination, on the regulation of dynorphin biosynthesis in the striatum. the study was done using male fisher-344 rats subjected to a regimen of subchronic administration of lithium chloride (4 meq/kg/day for 1,2,4 or 6 days, i.p.) or a regimen of chronic oral administration of a diet containing lithium carbonate (1.5 g/kg of the diet). subchronic administ ...19882898269
clinical utility of thyrotropin-receptor antibody assays: comparison of radioreceptor and bioassay methods.thyrotropin (thyroid-stimulating hormone or tsh)-receptor antibodies, important in the pathogenesis of graves' disease, can be assayed by one of two methods: (1) bioassays that measure stimulation of thyroid cellular activity by patient immunoglobulins or (2) radioreceptor assays that measure inhibition of binding of labeled tsh to tsh receptors by the same substances. in this study, we report our experience with bioassay of thyroid-stimulating immunoglobulins (tsi) based on measurement of gener ...19882898572
dual secretory and myoepithelial differentiation in the transplantable r3230ac rat mammary carcinoma.the hormone-responsive r3230ac mammary carcinoma, serially transplantable in fisher rats, shows striking functional and morphological similarities to the normal mammary gland. we have studied its cellular composition by both light and electron microscopy, employing markers of myoepithelial and epithelial cells. we identified two cell types: the major cellular component corresponded to epithelial milk-protein secreting cells, while a second component showed immunocytochemical and ultrastructural ...19882898832
activation of mhc-restricted rat t cells by cloned syngeneic thyrocytes.we have previously demonstrated that rat thyrocytes express mhc class ii ag (rt1.b&d) in response to ifn-gamma. to determine whether mhc class ii-positive thyrocytes can be recognized by mhc-restricted t cells, we used our clone of rat thyroid cells (1b-6) derived from the fisher rat thyroid cell line (frtl-5) and known to express mhc class ii ag in response to recombinant rat ifn-gamma. cd4+ and cd8+ normal syngeneic fisher rat spleen t cells were selected by flow cytometry and averaged greater ...19882899600
balsalazide in the maintenance treatment of patients with ulcerative colitis, a double-blind comparison with sulphasalazine.balsalazide (bsz) is a pro-drug which releases 5-aminosalicylic acid (5asa) and 4-aminobenzoyl-beta-alanine (an inert carrier) in the colon of various species including man. bsz was compared with sulphasalazine (sasp) (both 1 g b.d. orally) in the maintenance of remission in patients with ulcerative colitis (uc). seventy-nine patients (53 male, 26 female), mean age 49 years (range 19-79 years), with uc were randomly allocated to either treatment (41 bsz, 38 sasp) for 6 months. the groups were si ...19882908755
retinal light damage reduces autofluorescent pigment deposition in the retinal pigment epithelium.lipofuscin in the retinal pigment epithelium (rpe) is thought to be derived from phagocytosed photoreceptor outer segment disc membranes. based on this hypothesis, one would predict that the rate of lipofuscin deposition in the rpe would be proportional to the density of photoreceptor cells in the retina. in previous studies it was demonstrated that specific loss of photoreceptor cells due to a genetic defect resulted in a substantial decrease in the rate of age-related lipofuscin accumulation i ...19892912913
calculation of transmitral flow by doppler echocardiography: a comparison of methods in a canine model.although several doppler echocardiographic methods for measuring transmitral flow have been described, the optimal method for calculation of flow remains unclear. seven time/shape combinations were tested in an experimental preparation in which mitral flow could be precisely controlled and measured. annular shape was considered to be either circular or elliptical, and the mitral orifice area was calculated from the anteroposterior and/or the mediolateral dimension(s) recorded at early and middia ...19892916413
response of rat alveolar macrophages to ozone: quantitative assessment of population size, morphology, and proliferation following acute exposure.the purpose of this study was to evaluate the in vivo effects of an acute exposure to low levels of ozone on rat pulmonary alveolar macrophages (pam). fisher 344 rats exposed to 0.0, 0.12, 0.8, or 1.5 ppm o3 for 6 h were killed immediately after and 3, 18, 42, or 66 h after ozone exposure and their lungs were lavaged. compared to sham-exposed (control) rats, exposure to 0.12 ppm o3 had no measurable effect on the total number, labeling index (li), mitotic index (mi), or morphology of rat alveola ...19892917543
fiber number and type composition in extensor digitorum longus, soleus, and diaphragm muscles with aging in fisher 344 rats.histochemical (m-atpase) fiber typing was done on extensor digitorum longus, (edl), soleus (sol), and diaphragm (dia) muscles of barrier-reared fisher 344 rats obtained at four different ages (3, 9, 28, and 30 months) from the colonies of the national institute of aging. in the edl there are no differences in the percent of type i fibers among the four age groups. the percent of type iia and iib fibers also showed no difference between the 3 and 30 month age groups. there was no apparent trend f ...19852931475
dopaminergic modulation of the diethylstilbestrol-induced proliferation of the anterior pituitary gland of the fisher 344 rat.bromocriptine (cb 154), a potent agonist upon the d-2 receptor, blocks the proliferation of the mammotrophs and the elevation of serum prolactin concentration induced by chronic exposure of male fisher 344 rats to diethylstilbestrol (des). fluphenazine, a dopamine receptor antagonist, blocks these effects of cb 154. we conclude that a d-2 receptor occurs on the proliferating mammotroph of the male fisher 344 rat and that stimulation of this receptor inhibits prolactin release and stops the des-i ...19852932654
a myosin heavy-chain-like polypeptide is associated with the nuclear envelope in higher eukaryotic cells.a high molecular weight polypeptide, identified as an atpase subunit by direct ultraviolet photoaffinity labeling, has been shown to be a component of nuclear envelope-enriched fractions prepared from a variety of higher eukaryotes (berrios, m., g. blobel, and p. a. fisher, 1983, j. biol. chem., 258:4548-4555). in rat liver as well as drosophila melanogaster embryos, this polypeptide appears to be a form of myosin heavy chain. this conclusion is based on both immunochemical and immunocytochemica ...19862943745
polysaccharide surface antigens expressed by nonmucoid isolates of pseudomonas aeruginosa from cystic fibrosis patients.we tested nonmucoid pseudomonas aeruginosa isolates obtained from cystic fibrosis (cf) patients for the expression of lipopolysaccharide (lps) serotype antigens, serum sensitivity, and production of mucoid exopolysaccharide (mep). when all nonmucoid isolates were compared with a set of random mucoid isolates, 20 of 52 (38%) nonmucoid isolates were typable and serum resistant, compared with 13 of 51 (24%) mucoid isolates (p = 0.16 by chi-square analysis). however, nonmucoid strains from cf patien ...19862943759
mechanical and histochemical characterization of skeletal muscles from senescent rats.maximal shortening velocity (vmax) and isometric tension (po) were measured in living fiber bundles and skinned fibers from extensor digitorum longus (edl) and soleus (sol) muscles of young adult (9 mo) and senescent (30 mo) fisher 344 rats. the fiber type composition of each muscle preparation was determined using myosin (m)-atpase histochemistry. vmax, determined by the slack test method, was unchanged in the edl but was increased in the sol muscles of young adult vs. senescent rats. velocitie ...19862944390
lambda cro repressor complex with or3 dna: 15n nmr observations.15n nmr studies of the coliphage lambda cro repressor are presented. the protein has been uniformally labeled with 15n, and individual amino acids have been incorporated. although the four c-terminal residues (63-66) were not located in the original crystallographic studies of the protein [anderson, w.f., ohlendorf, d.h., takeda, y., & matthews, b.w. (1981) nature (london) 290, 754], it has been proposed that the c-terminus is involved in dna binding [ohlendorf, d.h., anderson, w.f., fisher, r.g ...19872962634
paradoxical organ-specific adaptations to streptozotocin diabetes mellitus in adult male fisher rats injected with streptozotocin (stz) to produce diabetes mellitus demonstrated a significant loss of total body weight associated with adipose and muscle tissue wasting. paradoxically, intestinal mass and length were increased in stz-treated rats despite catabolism of other tissues. concomitant with increased intestinal mass, food and water intake increased significantly in stz-diabetic animals. renal weight was not reduced despite the fall in total body weight. it is propos ...19882967640
renal phosphate transport in humoral hypercalcemia of malignancy.fisher rats bearing the h-500 leydig cell tumor (lct) develop humoral hypercalcemia of malignancy (hhm), which is accompanied by hypophosphatemia and hyperphosphaturia. to better define the mechanisms underlying the changes in phosphate metabolism, the activity of sodium-dependent phosphate uptake (na+-pi) in microvillus membrane vesicles (mmv) isolated from the renal cortex of lct-bearing rats was studied. ten days after tumor transplantation the animals became hypercalcemic, hypophosphatemic, ...19882974244
testicular lh receptors during aging in fisher 344 rats.levels of serum lh, prolactin, testosterone, progesterone and 17-oh progesterone and the testicular concentration and total content of lh receptors were measured in 4-, 11-, 18-, and 27-month-old fisher 344 rats. all 27-month-old rats had leydig cell tumors. at first, testicular lh receptor levels decreased with age, but with the appearance of the testicular tumors, these levels increased dramatically. serum prolactin levels fluctuated with age, but were significantly decreased in 27-month-old r ...19852982778
stability and utility of the unique human small cell carcinoma line shp-77.the human small cell (oat cell) carcinoma line, shp-77, established by fisher and paulson in 1977 and originally described as a "large cell variant of oat cell cancer" has been evaluated by several different parameters and shown even after more than 200 passages to retain properties described for the original cell line. karyotypic, histological, and biochemical features are retained, as well as tumorigenicity in nude mice. the original authors' suggestion that this is a propitious cell line for ...19852985251
the simian virus 40 sequences between 0.169 and 0.423 map units are not essential to immortalize early-passage rat embryo cells.f8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. we show that cloned f8dl dna immortalized early-passage fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 dna. in contrast, we detected no immortalized colonies when we transfected the cells with dna of five other early-region deletion mutants that do not make stable truncated forms of t antigen. since all five of these mutants ...19852987680
hepatic neoplastic nodules, adenofibrosis, and cholangiocarcinomas in male fisher 344 rats fed corn naturally contaminated with fusarium moniliforme.twelve male fisher 344 rats were fed corn naturally contaminated with fusarium moniliforme. this corn sample was obtained from feed being fed horses during an outbreak of leukoencephalomalacia. all rats necropsied from 123 to 176 days post feeding had multiple hepatic nodules and pale depressed hepatic areas. histological examination revealed multiple hepatic neoplastic nodules and large areas of adenofibrosis and cholangiocarcinomas. the corn only diet was deficient in many nutrients including ...19852990756
2,4-dichlorophenoxyacetic acid (2,4-d) does not cause polyneuropathy in the rat.2,4-dichlorophenoxyacetic acid (2,4-d), a component of agent orange, was injected intraperitoneally into adult male fisher rats for 3-12 weeks. during the period of study gait and toe-spreading reflexes remained normal and distal motor latencies, motor and mixed nerve conduction velocities and amplitudes remained similar (p greater than 0.05) in animals receiving 2,4-d or vehicle. this study suggests that 2,4-d is not toxic to peripheral nerves in the rat.19852997403
selective inhibition of leukotriene b4 biosynthesis in rat pulmonary alveolar macrophages by dietary selenium deficiency.weanling male fisher 344 rats were maintained on low selenium basal and se-supplemented diets for 38 weeks. a several fold reduction in the glutathione peroxidase activity of the lung and liver tissues in rats maintained on low se basal diet established their se-deficient status. analysis of the supernatants from resting pulmonary alveolar macrophage suspensions showed negligible extracellular release of pge2, txb2 and ltb4 in both diet groups. a challenge with opsonized zymosan particles increa ...19852998361
effect of age on beta adrenergic relaxation of the rat jugular vein.using the fisher 344 rat model and blood vessel ring segments in vitro, age-related changes in vascular beta adrenergic relaxation were investigated. in the pulmonary artery and aorta, maximum isoproterenol-induced relaxation and sensitivity to isoproterenol declined from 1 to 3 months of age confirming previous reports. in animals 6 months of age, these vessels no longer relaxed to isoproterenol. in the jugular vein, in which beta adrenergic mechanisms predominate, there was no change in maximu ...19863001294
morphine dependence and protracted abstinence: regional alterations in cns radioligand binding.rats (fisher f-344) were given free access to a 10% sucrose solution containing 0.5 mg/ml morphine sulfate (controls received the sucrose vehicle only) as their sole source of fluid. daily morphine intake averaged 119 +/- 21 mg/kg, an amount sufficient to induce physical dependence. after 18 days on this regimen, the control and dependent subjects were sacrificed. a protracted abstinence group was weaned from morphine by reducing its concentration in the vehicle by 20% over the next 5 days, foll ...19853001786
polyomavirus-transformed fr 3t3 rat cells are able to form metastases in syngeneic rats.a series of polyomavirus-transformed fr 3t3 rat cell lines were tested for their tumorigenic and metastatic properties after subcutaneous inoculation of syngeneic fisher rats. all of them grew into tumors, which appeared with variable latency periods; the td50 varied from cell line to cell line. eight of the 18 transformants that were inoculated gave rise to metastases, always localized in the lung. the capacity to form metastases, though at a low frequency, was also conferred on fr 3t3 cells up ...19863002027
short- and long-term evaluation of normal and abnormal human thyroid cells in monolayer culture.we have investigated the tsh responsiveness of normal and abnormal human thyroid cells cultured in the short term with high serum concentrations and for up to 6 months in a low serum, chemically-defined, medium. cells from normal human thyroid tissue (n = 9), multinodular goitre (n = 6), benign follicular adenomata (n = 6), and differentiated thyroid carcinoma (n = 3) formed confluent monolayers which were sensitive to bovine tsh (btsh) in concentrations greater than 25 microu/ml when assessed b ...19853002667
control of phosphorylase kinase in the isolated glycogen particle by ca2+-mg2+ synergistic activation and camp-dependent phosphorylation.the isolated glycogen particle provides a means to examine the regulation of glycogen metabolism with the components organized in a functional cellular complex. with this system, we have studied the control of phosphorylase kinase activation by ca2+ and camp. contrary to a previous report (heilmeyer, l. m. g., jr., meyer, f., haschke, r. h., and fisher, e. h. (1980) j. biol. chem. 245, 6649-6656), phosphorylase kinase became activated during incubation of the glycogen particle with mgatp2- and c ...19863007504
hcg-induced tsh receptor activation and growth acceleration in frtl-5 thyroid cells.our previous studies have indicated specificity cross-over between lh/hcg and the tsh receptor. we have now analyzed the ability of the tsh-dependent fisher rat thyroid cell line (frtl-5) to proliferate in a differentiated state under the influence of highly purified hcg (hcg-cr121). tsh receptor activation and growth induction were observed after 7 days suspension from a tsh-induced growth phase. the effect of 10 ug hcg-cr121 was equivalent to 500 +/- 43 (mean +/- sem) uiu of human tsh (2nd irp ...19863009152
human immunodeficiency virus seroprevalence in pediatric patients 2 to 14 years of age at mama yemo hospital, kinshasa, zaire.seroprevalence to human immunodeficiency virus (hiv) was determined among 368 children 2 to 14 years of age who were admitted to the pediatric service at mama yemo hospital in kinshasa, zaire. forty (11%) of these patients and only one (1%) of 92 healthy siblings of these patients were hiv seropositive (chi 2 = 8.68, p less than .01). seropositivity was associated with previous hospitalization, receipt of a blood transfusion prior to the current hospitalization (odds ratio 3.1; 95% confidence in ...19863020492
foodborne snow mountain agent gastroenteritis in a school 1984, an outbreak of gastroenteritis occurred at a school with 1,860 students in brooklyn, ny. in a single-stage cluster sample of 375 students, 129 (34%) had illnesses that met our case definition of vomiting or diarrhea. the mean incubation period was 26 hours, and the mean illness duration was 24 hours. all case students had eaten in the cafeteria on at least one day between nov 13 and 16, compared with 174/214 (81%) noncase students (p = 10(-8), fisher exact test). foods implicated were f ...19873029660
protection from ischemia-induced cerebral edema in the rat by u-50488h, a kappa opioid receptor agonist.u-50488 is a specific kappa opioid agonist which produces in rats water diuresis resulting in an elevation of plasma osmolarity. pretreatment with u-50488h (the methanesulfonate salt) in fisher rats prior to 4 h of bilateral carotid occlusion prevented the development of edema in the forebrain, and the effect was greater than that from pentobarbital anesthesia. an additional injection of an antidiuretic hormone which prevented the plasma hyperosmolarity also significantly reduced the anticerebra ...19873030502
cellular mutation mediates t-antigen-positive revertant cells resistant to simian virus 40 transformation but not to retransformation by polyomavirus and adenovirus type 2.t-antigen-positive transformation revertant cell lines were isolated from fully simian virus 40 (sv40)-transformed fisher rat embryo fibroblast cells (ref 52 cells) by methionine starvation. reversion of the transformed cells (sv-52 cells) was caused by a mutation within the cellular genome. to demonstrate this, we isolated sv40 dna from the host genome, inserted it into plasmid pspt18 dna, cloned it in escherichia coli, and microinjected it into the nuclei of the ref 52 cells. fully transformed ...19873033314
cloning the fisher rat thyroid cell line (frtl-5): variability in clonal growth and 3,'5'-cyclic adenosine monophosphate response to investigate the stability of frtl-5 cells we cloned stock cells by limiting dilution in a 6-hormone medium with bovine tsh (btsh) (10 mu/ml) (6h medium). one third of the wells with irradiated fibroblast feeder cells developed frtl-5 colonies. one hundred and twenty clones were obtained, of which 29 (8%) developed sufficiently for functional analysis. clones were tested for growth responses to btsh stimulation as evidenced by 72-h [3h]thymidine uptake and tsh receptor activation by camp gener ...19873036476
age-dependent changes in rat brain mitochondria of synaptic and non-synaptic origins.synaptic and non-synaptic mitochondria were isolated from the brains of 3- 12-and 28-30-month-old female fisher 344 rats. total oxygen consumption and oxygen consumption due to mitochondrial respiration decreases 83% with increasing age in synaptic mitochondria using malate plus glutamate as substrate, but only 33% in non-synaptic mitochondria; succinate-driven activity is not affected. succinate-driven superoxide generation decreases over 90% in both fractions; malate plus glutamate-driven supe ...19873037204
on the fidelity of dna replication. isolation of high fidelity dna polymerase-primase complexes by immunoaffinity chromatography.error rates for conventionally purified dna polymerase-alpha from calf thymus, chicken, and human sources have been reported to be one in 10,000 to one in 40,000 nucleotides incorporated. isolation of polymerase-alpha by immunoaffinity chromatography yields a multiprotein high molecular weight replication complex that contains an associated dna primase (wong, s. w., paborsky, l. r., fisher, p. a., wang, t. s-f., and korn, d. (1986) j. biol. chem. 261, 7958-7968). we have isolated dna polymerase- ...19873038898
relationship between in vivo airway reactivity and in vitro responsiveness of tracheal smooth muscle in inbred rats.we evaluated the in vivo and in vitro airway responses to cholinergic stimulation in two highly inbred strains of rats. five fisher and six lewis rats inhaled aerosols of doubling concentrations of methacholine (mch). pulmonary resistance (rl) was measured before and after each mch inhalation. the concentration of mch required to double rl (ec200rl mch) was calculated. spirally cut tracheal strips were suspended in an organ bath and the isometric tension was recorded. cumulative concentration-re ...19883068743
pre- and postsynaptic markers of cholinergic neurons in the cerebral cortex of rats of different order to investigate the effect of aging on postsynaptic muscarinic binding, we measured 3h-pz binding in the cerebral cortex of male fisher 344 rats of different ages. chat activity was measured as a presynaptic marker of the cholinergic system. total and salt-soluble ache activities were also measured. maximal binding of 3h-pz and chat activity did not change during aging. the ratio of detergent-soluble ache to salt-soluble ache was lower in 30-month-old rats than in 4-month-old rats. the r ...19883074925
in vitro and in vivo regulation of liver epithelial cells carrying a metallothionein-rast24 fusion gene.we inserted a zinc-responsive metallothionein-rast24 fusion gene in both orientations into a retroviral vector (svx) and infected fisher rat liver epithelial cells. only the construction in which the viral long terminal repeat and the metallothionein promoters were in opposite orientations resulted in cell lines with biologically altered behavior. two lines (mtr-1 and mtr-6) showed altered morphology in vitro when znso4 was added to the medium. these cell lines grew in soft agarose in a dose-dep ...19883076453
structural analysis and immunogenicity of pseudomonas aeruginosa immunotype 2 high molecular weight polysaccharide.we analyzed high molecular weight polysaccharide (ps) from the fisher immunotype 2 (it-2) strain of pseudomonas aeruginosa for molecular composition and structure, then determined its immunogenicity in healthy adults. the ps was composed of 2-acetamido-2,6-dideoxygalactose (n-acetyl fucosamine) and glucose in a molar ratio of 2:1. structural analysis by carbon-13 and proton nuclear magnetic resonance confirmed that the high molecular weight ps was structurally identical to that of the o-specific ...19863080477
adherent spleen cell production of e series prostaglandins in rats bearing variants of the r3327 dunning prostatic adenocarcinoma: effect of cyclophosphamide.prostaglandins of the e series (pge) have been implicated in many facets of immunoregulation, as well as having a possible role in metastatic dissemination. variant sublines of the dunning r3327 rat prostatic adenocarcinoma, differing in growth rate, hormonal responsiveness and in propensity for metastasis, were carried in fisher x copenhagen f1 animals. adherent spleen cells were assayed in vitro for their ability to convert arachidonic acid to prostaglandins of the e series. these glass adhere ...19863088126
[ir-gene control of rat t-lymphocyte proliferation in response to ig allotype].antigen-induced t-cell proliferation in vitro was adapted to the estimation of antiallotypic response to igk-ib immunoglobulin k chain allotype of msu/b and fisher rats in wag, august and fi (wag x august) rats. august and fi rat t lymphocytes responded to igk-ib alloantigen with stimulation indexes (si) 3.8-5.0 (high responders), while wag rat t lymphocytes showed practically no response (si 0.9-1.8--low responders). these results correlate well with our previous findings of ir-gene-controlled ...19863089344
polyclonal and monoclonal antibody therapy for experimental pseudomonas aeruginosa pneumonia.a human immunoglobulin g preparation, enriched in antibodies to lipopolysaccharide (lps) pseudomonas aeruginosa antigens (pa-igiv) and murine monoclonal antibodies (mab) to p. aeruginosa fisher immunotype-1 (it-1) lps antigen and outer membrane protein f (porin), were evaluated for therapeutic efficacy in a guinea pig model of p. aeruginosa pneumonia. the concentration of antibodies to it-1 lps was 7.6 micrograms/ml in pa-igiv and 478 micrograms/ml in the it-1 mab preparation. no antibody to it- ...19863093385
influence of complex charge and size on the uptake of 99mtc-diphosphonates in osteogenic tissue.the biodistributions of six chromatographically pure 99mtc-hedp complexes have been determined in soft tissues, normal bone and osteogenic lesions (induced with a walker 256 tumor) in fisher 344 rats. the physical properties of each 99mtc-hedp complex including anionic charge, partial molar volume, molecular weight and spectral characteristics are known; thus allowing structure-activity relationships to be drawn. the results indicate that the smallest, low charged, mononuclear 99mtc-hedp complex ...19863095270
cross-reactions of lipopolysaccharides of pseudomonas aeruginosa in antipneumococcal and other antisera.lipopolysaccharides of the seven fisher immunotypes of pseudomonas aeruginosa gave cross-precipitation in many antipneumococcal sera. the reaction of pseudomonas type iv in type 25 antipneumococcal serum was immediate and heavy: 93 micrograms of antibody nitrogen per ml. correlations are described, mainly between the structures of the o-chains of the immunotypes and their specificities as shown by the cross-reactions.19863096896
the mobility of concanavalin a receptors and surface immunoglobulins on rat hepatocyte plasma membranes.lateral mobilities of lectin receptors and surface immunoglobulins were measured in plasma membranes of hepatocytes prepared by smearing small pieces of rat liver tissue and then using the fluorescence recovery after photobleaching (frap) technique. smears were treated with various doses of fluorescein isothiocyanate (fitc) conjugated concanavalin a (cona), succinylated cona (scona), wheat germ agglutinin (wga), and soybean agglutinin (sba), as well as with rabbit anti-rat igg (rara/igg) and goa ...19873098295
reduction of local tumor recurrence by excision with the co2 laser.this study was undertaken to determine the effect of co2 laser surgery on the incidence of local recurrence following tumor resection. thirty female fisher 344 rats were implanted with r3230ac mammary carcinoma. all tumors were completely excised 18-21 days post-implantation. in 15 animals, the resection was done with a scalpel. fifteen animals underwent laser excision with the sharplan 733 co2 laser (temoo, 125 mm handpiece, 25w continuous wave). primary tumor size was 17.6 +/- 1.4 mm in the sc ...19863100889
the use of monoclonal antibodies in the diagnosis of partially treated pseudomonas aeruginosa meningitis: patient report.the objective of this study was to evaluate the efficacy of mouse anti-pseudomonas monoclonal antibodies to detect small numbers of pathogenic pseudomonas in the spinal fluid of a patient who had been treated with antibiotics for meningitis. anti-605 monoclonal antibody against pseudomonas aeruginosa pac 605, a rough mutant, showed sensitivity similar to that of anti-fisher 1 monoclonal antibody in detecting p. aeruginosa fisher 1 antigens in boiled spinal fluid drawn from a patient with a p. ae ...19863103793
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