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comparison of fetal rabbit brain xenografts to three different strains of athymic nude rats: electrophysiological and immunohistochemical studies of intraocular grafts.interest in the use of neural tissue transplantation for the study of cns development and maturation and the potential use of this technique for the treatment of certain degenerative cns disorders has led to our use of transplantation of neural tissue across species lines. prior to extensive transplantation studies using athymic rats as recipients, we wished to evaluate the currently available strains of athymic rat for their suitability as host animals for xenografts of neural tissue. fetal cer ...19921344293
changes in myocardial beta-adrenergic receptors during acute rejection of heterotopically transplanted rat hearts.to evaluate changes of the myocardial beta-adrenergic receptors in acute cardiac graft rejection, the density and binding affinity value of the myocardial beta-adrenergic receptors in heterotopically transplanted rat isografts and allografts were analyzed. hearts from fisher rat donors were transplanted either to the fisher rats (isografts) or to lewis rats (allografts). histologic examination of the allografts showed mild to moderate rejection on the seventh and fourteenth days and showed sever ...19921333553
cellular tropism and localization in the rodent nervous system of a neuropathogenic variant of friend murine leukemia virus.we studied pvc-211 murine leukemia virus (mulv) (1), a neuropathogenic variant of friend mulv, to determine its cellular tropism and distribution in the nervous system of infected rats and the factors that affected disease expression.19921405490
survival of normal and magnesium-deficient erythrocytes in rats: effect of magnesium-deficient diet vs. splenectomy.magnesium deficiency in rats causes anemia, the mechanism of which is unknown. the effect of dietary magnesium, splenectomy, and magnesium content of erythrocytes on erythrocyte survival was studied in fisher rats. half of the animals were splenectomized, the remainder sham-splenectomized; each group was subdivided, and some were placed on a magnesium-deficient diet, the rest on a control diet. after 6 weeks, each of the four subgroups was divided, half were given 51cr-labeled red cells from mag ...1978641390
endogenous intestinal metallothionein possibly contributes to the renal accumulation of cadmium.at low levels of dietary cadmium chloride, cadmium accumulates directly in the kidneys and not in the liver. as dietary cadmium induces intestinal metallothionein (mt), intestinal cdmt complexes could be at least partly responsible for the renal accumulation of oral cadmium. for this to be possible, however, serosal release of mucosal cdmt would be required. to test this hypothesis, we perfused isolated rat small intestinal segments (fisher-parsons method) in an attempt to demonstrate the releas ...19921303945
karyotypes of pneumocystis carinii from korean rats.molecular karyotyping was applied to pneumocystis carinii(pc) from two strains of experimental rats, sprague dawley(sd) and fisher(f), in korea. field inversion gel electrophoresis and contour clamped homogeneous electric field electrophoresis resolved 15 chromosomal bands from the pc. the size of the bands was estimated 270kb to 684kb from sd rats, and 273kb to 713 kb from f rats. the bands of 283 kb from sd rats and of 273 kb from f rats stained more brightly suggesting duplicated bands. total ...19921420031
synthetic capacity and cell metabolites of bile duct obstructed rat livers. effect of free and conjugated dihydroxy bile acids.morphologic alterations in liver cells after bile duct ligation are well known and documented in numerous reports. biochemical studies concerning metabolic changes in cholestatic liver are rare. therefore, in this study, liver cell metabolites and the capacity of the perfused cholestatic rat liver to produce glucose, urea and ketone bodies were measured. in addition the influence of dihydroxy bile acids on normal and bile duct ligated rat livers was studied. concentrations of adenine nucleotides ...19751199667
hepatic phenylalanine 4-monooxygenase is a phosphoprotein.phenylalanine hydroxylase [phenylalanine 4-monooxygenase; ec 1.14.16.1; l-phenylalanine, tetrahydropteridine:oxygen oxidoreductase(4-hydroxylating)] isolated from rat liver is a phosphoprotein containing approximately 0.31 mumol of protein-bound phosphate per mumol of subunit (50,000 molecular weight). when the enzyme is further phosphorylated in the presence of atp and a 3'5'-cyclic-amp-dependent protein kinase (ec 2.7.1.37; atp:protein phsophotransferase), an additional 0.7 mumol of phosphate ...19761064028
hepatic triiodothyronine sulfation and its regulation by growth hormone and triiodothyronine in rats.the regulatory mechanism of cytosolic sulfation of t3 has been studied in rat liver. sulfation of t3 is sexually differentiated in adult rats of sprague-dawley (sd), fisher 344, and aci strains. in sd strain, the male animals showed 4 times higher sulfating activity than did the females. the specific activity was decreased by hypophysectomy of male adult rats, but was not affected in the females. thus, the sex-difference was abolished in the hypophysectomized condition. supplement of human gh in ...19921429499
[development of resistance in fisher lymphadenosis cells to dipin and bruneomycin when these preparations are used separately and in combination].it was shown that dipin and bruneomycin resistant tumor cells appeared in mice with transplanted lymphadenosis after 10 passages on single use of the drugs. when the drugs were used in combination, no lymphadenosis cells resistant either to bruneomycin, or to dipin and their combination were found. the combined use of the drugs prevented development of resistance to them in the lymphadenosis cells at least during 10 passages on mice. the data on the possible prevention of the resistance developm ...19751211895
brain myelination in rats treated with ionizing radiation in utero.effects of ionizing radiation on brain myelination and some physical development parameters were studied in laboratory rats (fisher f-344 inbred strain). rats were treated with three different doses of radiation (150 rad, 15 rad, and 6.8 rad) delivered on the 20th day of prenatal life. exposure to 150 rad reduced body, brain, ovary, kidney, heart and spleen weights. prenatal exposure to 150 rad of radiation reduced the cerebral cortex weight by 22 percent at 30 days of age, and 20 percent at 52 ...19921401730
the effect of dexamethasone and endotoxin administration on biliary iga and bacterial adherence.adherence of bacteria to the intestinal epithelial cell may be the crucial initiating event for invasion and translocation and is normally prevented by both immune (iga) and nonimmune (mucus, peristalsis, desquamation) mucosal defense mechanisms. the purpose of the present study was to examine the effect of endotoxin administration on mucosal immunity and to define the role of glucocorticoids, commonly released during endotoxicosis, in this process. thirty female fisher rats were randomly assign ...19921434594
an evaluation of the role of leukocytes in the pathogenesis of experimentally induced corneal vascularization. iii. studies related to the vasoproliferative capability of polymorphonuclear leukocytes and lymphocytes.studies in the past have suggested that leukocytes are a prerequisite to corneal vascularization. to test this hypothesis further, experiments were conducted to determine whether the intracorneal instillation of polymorphonuclear leukocytes, lymphocytes, or components of leukocytes would induce a corneal vascular ingrowth. these cells or cellular fractions were injected intracorneally into fisher albino rats whose circulating leukocytes had been depleted by total body x-irradiation. polymorphonu ...19761247083
transcription and replication of animal mitochondrial dnas.the development of in vitro transcription and replication systems has allowed the identification of promoter sequences and origins of replication for several animal mtdnas. as a consequence, the necessary reagents and basic information are available to permit the characterization of transacting factors that are required for transcription and replication. all of the animal trans-acting species purified at this time are known or reasoned to be nuclear gene products. there is now the opportunity to ...19921452432
the detection of philadelphia chromosome negative metaphases in long-term bone marrow cultures of the peripheral blood from patients with chronic myeloid leukemia predicts response to interferon-alpha 2a.the cytogenetic response of 10 patients with chronic myeloid leukaemia (cml) to human recombinant interferon-alpha 2a (rhifn alpha 2a) was compared to the philadelphia chromosome (ph) status of the pre-treatment peripheral blood cells after in vitro culture under long-term bone marrow culture (ltbmc) conditions. pre-treatment light density peripheral blood cells were cultured in ltbmc on sex-mismatched irradiated allogeneic stromal layers with weekly cytogenic examination of metaphases in the no ...19921453769
[angiogenesis induced by rat glioma cells in vitro].angiogenesis induced by rat glioma cells was examined in vitro using a double chamber co-culture system. cultured microvascular endothelial cells from fisher 344 rat brain, rat c6 glioma cells and rat t9 gliosarcoma cells were used for this study. endothelial cells, cultured on type i collagen, formed capillary-like structures. in the co-culture system, c6 glioma cells promoted this formation. on the other hand t9 gliosarcoma cells had no effect on it. the supernatants of c6 glioma cells and t9 ...19921280347
the appearance and role of gamma delta t cells in the peritoneal cavity and liver during primary infection with listeria monocytogenes in rats.we have previously reported that gamma delta t cells play important roles in protection during the early stage of infection with listeria monocytogenes in mice. to generalize the protective roles of gamma delta t cells in listerial infection to different species, we examined the appearance of gamma delta t cells during infection with l. monocytogenes in fisher f344 rats. the numbers of bacteria in the peritoneal cavity and liver increased to a maximum level on day 3 and then decreased to an unde ...19921489731
a potential vaccine for cocaine abuse prophylaxis.presently, there are estimated to be 1.5 to 2.0 million individuals infected with hiv-1 in the u.s. and about 12 million worldwide. in the u.s. over 90% of reported cases of aids occurred among two subgroups, homosexual males and intravenous substance abusers (ivsas). currently, there is no anti-cocaine addiction medication available. in order to explore vaccination as an alternative means for protection against cocaine addition, we immunized inbred male fisher rats with either cocaine emulsific ...19921500284
elevated expression of thymidylate synthase in doxorubicin resistant human non small cell lung carcinomas.human non-small cell lung carcinomas of previously untreated patients were analyzed for expression of thymidylate synthase (ts) using immunohistochemistry. of the 94 tumors, 67 were positive for ts and 27 negative. a significant correlation between the expression of ts and the resistance to doxorubicin was found (p < 0.0001). eighty-four percent of the ts-positive tumors were resistant to doxorubicin, whereas of the 27 ts-negative tumors only 44 percent were resistant. a group of patients (n = 1 ...19921338282
augmentation of the systemic host anti-tumor response through laser excision.this study examines whether primary laser excision results in augmentation of the systemic host anti-tumor response to tumor rechallenge. single r3230ac mammary tumor implants, (0.5 x 0.5 x 1.0 mm), were grown in 112 female fisher 344 rats. the animals were randomized. group s tumors were excised by scalpel. group e was excised with a surgistat electrocautery (valley labs, boulder, co). group cs was excised with a sharplan 1100 co2 laser (sharplan, allendale, nj) at 25 watts (w) continuous wave ...19921508027
modification of tumor promotion in the mouse skin by exposure to an alternating magnetic field.some epidemiological studies have suggested that exposure to an alternating magnetic field may increase the incidence of some cancers. our earlier study of carcinogenesis in mouse skin, indicated that exposure to a magnetic field (mf) alone did not promote the growth of tumors. in the present experiment, the ability of a mf to act as a tumor copromoter was investigated. the dorsal skins of female sencar mice (6-7-weeks-old) were treated with 10 nmol of 7,12-dimethylbenzanthracene (dmba) to initi ...19921511404
effects of wheat bran on blood and tissue hormone levels in adult female rats.the protective effect of dietary fiber on breast cancer development might be explained by the interaction between dietary fiber and hormonal processes. we studied the effects of dietary fiber and the effects of a reduced energy intake on the exposure of mammary tissue to both estrogens and progesterone, as well as the blood plasma levels of these steroids and of lh and fsh. adult female fisher rats were fed ad libitum either a low-fiber diet (0.5% dietary fiber based on wheat flour) or a high-fi ...19921329421
effects of growth hormone on diaphragmatic recovery from malnutrition.a 25% weight loss was induced in adult fisher 344 rats by nutritional deprivation. subsequently, normal feeding was resumed. refed animals were divided into three groups and received recombinant human growth hormone (rhgh) injections during 5 wk of refeeding, saline injections during 5 wk of refeeding, or 9 wk of refeeding without injections. the effects of nutritional deprivation and the various refeeding protocols on the cross-sectional areas (csa) of each of the four types of myofibers [typed ...19921400040
photoactivated photofrin ii: astrocytic swelling precedes endothelial injury in rat brain.light activation of circulating hematoporphyrin derivatives has been used in the treatment of selected brain tumors. the effects of this photodynamic therapy on the non-neoplastic, adjacent brain tissue are incompletely characterized. we studied in adult fisher rats the time-dependent (1 hour to 7 days) effects of photoactivated photofrin ii. our protocol was comparable to that used in the treatment of human brain tumors. structural and functional changes spread from the treatment surface and fr ...19921531496
transport of deutherium oxide across isolated rat small intestine.1. transport of deuterium oxide from a luminal perfusate containing 1% d2o was studied in fisher & gardners (1974) isolated preparation of perfused rat small intestine. 2. the kinetics of appearance of d2o in the intestinal secretion at the serosal surface fitted well to a single exponential function. 3. the steady-state concentration of d2o in this secretion was not significantly different from the concentration in the luminal perfusate. 4. the total tissue water contained d2o at a concentratio ...19751177106
prevention of prosthetic vascular graft infection by rifampicin impregnation of a protein-sealed dacron graft in combination with parenteral cephalosporin.antibiotic-impregnated prosthetic vascular grafts have been shown to be highly resistant to bacterial contamination in animal models. the aim of this study was to assess if graft infection in an animal model challenged with local bacterial contamination could be reduced further by the use of rifampicin soaking of a protein-sealed dacron graft in addition to the prophylaxis provided by perioperative intravenous cephalosporin. of 20 merino sheep given cefoxitin intravenously before the induction o ...19921531988
the effect of light fluence rate in photodynamic therapy of normal rat brain.this paper reports the effect of incident light fluence rate on the depth to which necrotic lesions are produced by photodynamic therapy (pdt) in the brains of normal fisher rats. the rats were injected intraperitoneally with photofrin (12.5 mg kg-1) 48 h prior to pdt with a fixed incident fluence of 35 j cm-2. the treatment was performed at 10, 50, 100, and 200 mw cm-2 and also in a periodic manner (30 s "on" at 100 mw cm-2, 30 s "off"). the depth to which necrosis occurred was determined 24 h ...19921410268
transmission modes of pneumocystis carinii among rats observed by karyotype analysis.to observe the transmission patterns of karyotype of pneumocystis carinii (pc) by rat colonies, three strains of rats, sprague-dawley(sd), wistar(w) and fisher(f) from various animal vendors, were suppressed of their immunity by injection of methyl prednisolone. they were kept for 5 to 13 weeks in 3 different animal rooms, a, b, and c. the purified organisms were prepared in low melting point agarose gel by embedded lysis method for pulsed field gel electrophoresis. field inversion gel electroph ...19921297419
effects of the calcium channel blocker flunarizine on the hemodynamics and oxygenation of tumor microvasculature.flunarizine is a diphenylpiperazine calcium entry blocker that has been shown previously to increase tumor blood flow and sensitivity to radiotherapy via reduction in the radiobiologically significant hypoxic fraction. two mechanisms of action have been proposed previously (vasodilation, altered blood viscosity), but no studies have been performed to examine its mechanisms of action in vivo. such information would be invaluable in determining the role of flunarizine in multimodality approaches t ...19921410275
early british discoveries in human genetics: contributions of r.a. fisher and j.b.s. haldane to the development of blood groups. 19921550114
neurotransmitter-induced hypothalamic-pituitary-adrenal axis responsiveness is defective in inflammatory disease-susceptible lewis rats: in vivo and in vitro studies suggesting globally defective hypothalamic secretion of corticotropin-releasing hormone.the susceptibility of female lewis (lew/n) rats to the development of streptococcal cell wall (scw)-induced arthritis and other autoimmune phenomena is associated with the inability of their hypothalamic-pituitary-adrenal (hpa) axis to adequately respond to inflammatory stimuli. in contrast, resistance to the development of scw-induced arthritis and other inflammatory autoimmune manifestations in histocompatible female fischer rats (f344/n) is related to their intact hpa axis response to inflamm ...19921350069
angiogenic activity of the k-fgf/hst oncogene in neural transplants.using retrovirus-mediated gene transfer into neural transplants, we have expressed the human k-fgf/hst oncogene in the central nervous system. single-cell suspensions of fetal rat brains were removed at embryonic days 13 and 14, exposed to a retroviral vector encoding the k-fgf oncogene and stereotaxically implanted into the caudate putamen of syngenic adult fisher rats. recipient animals were sacrificed at intervals of 6-16 months without evidence of neurological impairment. mock-infected graft ...19921375717
response to alarm substance in different rat strains.two studies were conducted. in the first study, four strains of male rats--long-evans (le), sprague-dawley (sd), wistar, fisher 344--were immersed in fresh water or water soiled by a rat of the same strain which presumably contains an alarm substance. le rats were less immobile than sd, wistar, and fisher rats in fresh water. all strains were significantly less immobile in soiled water; however, relative to immobility times in fresh water, the decrease in immobility in soiled water was greatest ...19921557445
difference of rejection between heart and heart-lung transplantation in rats: flowcytometric analysis of graft infiltrating lymphocyte subsets.reported clinical and experimental observations indicate that heart grafts in combined heart-lung transplantation are less frequently rejected than heart grafts transplanted alone. in order to elucidate the mechanism of this difference, twenty-eight inbred male lewis rats receiving heterotopic allografts from inbred male fisher rats were evaluated for surface markers of graft infiltrating lymphocytes (gil) and peripheral blood lymphocytes (pbl) using flowcytometry. monoclonal antibodies investig ...19921561904
glutamine facilitates chemotherapy while reducing toxicity.dose intensification of chemotherapy is thought to increase survival. with recent advances in hemopoietic cell modulators such as granulocyte colony stimulating factor, the limiting toxicity of intensifying chemotherapeutic regimens has become the severity of the associated enterocolitis. in animal models, glutamine protects the host from methotrexate-induced enterocolitis. this study evaluates the effects of a glutamine-supplemented diet on the tumoricidal effectiveness of methotrexate. sarcoma ...19921287230
angiopolarity of cell carriers: directional angiogenesis in resorbable liver cell transplantation devices.the purpose of this study was to obtain directional angiogenesis of small blood vessels and capillaries to an implant made from a resorbable polymer for hepatocyte transplantation. it was intended to mimic the native acinar structure of the liver in order to facilitate replication of the cells and organ growth. the implant device structure was designed for injection to minimize surgical trauma. hollow microspheres with an open porous wall structure and one large central opening were made from po ...19921377553
[tumor ablation with focalized ultrasound. in vivo experiment with prostatic adenocarcinoma r3327 mat-ly-lu].ultrasound can induce tissue lesions by a combination of thermal and mechanical effects related to the tissue absorption of the energy emitted at the focal point of the transducer. the effects of focused ultrasound were studied in vivo in fisher/copenhagen hybrid rats bearing dunning r3327 experimental prostatic carcinoma. the experimental tumour was transplanted by subcutaneous injection into the abdomen of 20 mg of tumour tissue derived from the mat-ly-lu strain. treatment was performed under ...19921302056
the impact of fk506 on graft coronary disease of rat cardiac allograft--a comparison with cyclosporine.we studied the impact of fk506, a potent immunosuppressant, on graft coronary disease and graft-infiltrating lymphocyte subset after rat heart transplantation. fisher rat heart grafts transplanted into lewis rat recipients were divided into three groups: control (n = 7), rats treated with fk506 at a dose of 0.32 mg/kg/day intramuscularly (n = 7), and rats treated with cyclosporine at a dose of 10 mg/kg/day intramuscularly (n = 7). grafts were removed on day 71 in the treated groups and on reject ...19921379829
immunization with extracellular proteins of mycobacterium tuberculosis induces cell-mediated immune responses and substantial protective immunity in a guinea pig model of pulmonary tuberculosis.we have studied the capacity of a selected fraction of mycobacterium tuberculosis extracellular proteins (ep) released into broth culture by mid-logarithmic-growth-phase organisms to induce cell-mediated immune responses and protective immunity in a guinea pig model of pulmonary tuberculosis. guinea pigs infected with m. tuberculosis by aerosol but not uninfected control guinea pigs exhibit strong cell-mediated immune responses to ep, manifest by dose-dependent cutaneous delayed-type hypersensit ...19921398989
infection-induced airway fibrosis in two rat strains with differential susceptibility.chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. by stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. however, in patient this condition is most typically found in lungs damaged by other factors, such as smoking, abnormal secretions, or barotrauma. we report the characterization of mycoplasma pulmonis infection-induced lung fibrosis in two immunocompetent rat strains with no pree ...19921612760
susceptibility of various animals to pneumocystis carinii infection.pneumocystis carinii (pc) is an important opportunistic pathogen of immune compromised hosts, and is known to infect various animals. the present study observed the infection status of 6 mammals and 3 strains of albino rats with pc after suppression of their immunity. methyl-prednisolone was injected once a week and tetracycline was supplied with water for 5 to 21 weeks. hamsters, guinea pigs, rabbits, dogs, cats and pigs were negative by impression smear, and only the rats were found infected b ...19921297418
comparison of the effects of wyeth-14,643 in crl:cd br and fisher-344 rats.wyeth-14,643 (wy) belongs to a diverse class of compounds which have been shown to produce hepatic peroxisome proliferation and hepatocellular carcinoma in rodents. based on a review of bioassay data, a relationship appears to exist between peroxisome proliferating compounds and leydig cell adenoma formation. most rat bioassays with peroxisome proliferators have been conducted in the fisher-344 (f344) rat, which has a high spontaneous incidence of leydig cell adenomas. thus, it was necessary to ...19921426718
sn-chlorin e6 antibacterial immunoconjugates. an in vitro and in vivo analysis.monoclonal antibody-sn-chlorin e6 immunoconjugates were prepared by the site-selective covalent modification of the monoclonal oligosaccharide moiety. by carefully controlling the reaction conditions and introducing triethanolamine groups as axial ligands of the sn moiety, conjugates with in vivo biodistribution properties similar to underivatized igg were prepared. by varying the reaction conditions, conjugates were reproducibly prepared with a range of photosensitizer to mab molar ratios from ...19921431166
5,10-methylenetetrahydro-5-deazafolic acid and analogues: synthesis and biological activities.the synthesis of 5,10-methylene-5-deazatetrahydrofolic acid (2), a stable, rigid analogue of 5,10-methylenetetrahydrofolate (1), is reported as a potential inhibitor of thymidylate synthase. the target compound was obtained by a fisher-indole type cyclization of the hydrazone 16 from 2-amino-6-hydrazino-4-oxopyrimidine (10) and diethyl n-[4-(3-formyl-1-pyrrolyl)benzoyl]-l-glutamate (15) followed by catalytic reduction of the product 17. similarly, modification of the fisher-indole type cyclizati ...19921433179
[the physiological reactions to stress in 3 strains of rats].we have studied the resistance of wistar, august, fisher male rats to 2- and 24-hours immobilization by vegetative and behavioral reactions. the difference in the resistance has been observed both between the three lines of rats and within one particular line. the lowest resistance to stress has been demonstrated by fisher rats, the highest by wistar rats. the correlation between emotional reactivity of rats and their response to stress has been shown.19921467465
cooperation of oncogenes in cell transformation and sensitization to killing by the parvovirus minute virus of mice.the established line of normal fisher rat fibroblasts (fr3t3) is naturally resistant to the parvovirus minute virus of mice (mvm), and was used as a model system to study the influence of stepwise transformation on the susceptibility of cells to this virus. when transformed with genes encoding the class i nuclear oncoproteins large t antigen of polyomavirus (pylt) or v-myc, cells retained a normal appearance, but acquired some ability to form colonies in soft agar. on the other hand, the class i ...19921645140
modulation of the carbohydrate moiety of thyroglobulin by thyrotropin and calcium in fisher rat thyroid line-5 cells.thyroglobulin secreted in the medium by fisher rat thyroid line-5 (frtl-5) cells cultured in the presence of thyroid stimulating hormone (tsh) shows a slower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a higher density position in a cscl gradient than thyroglobulin secreted by frtl-5 cells cultured in the absence of tsh for 5-7 days. such a tsh effect is much less or not evident when secreted thyroglobulin is digested with peptide n-glycohydrolase f ...19921370485
hepatitis b virus infection markers in genetic haemochromatosis. a study of 272 patients.serum hepatitis b virus (hbv) infection markers were studied in 272 patients with homozygous genetic haemochromatosis complicated (n = 33) or not (n = 239) with primary liver cancer (plc). controls consisted of 255 029 healthy blood donors from whom age- and sex-matched control groups were extracted for statistical evaluation using the fisher exact test. in blood donors, hbsag was positive in 0.075% of males and 0.04% of females. this population was not screened for anti-hbs. anti-hbc alone (wit ...19911667016
metabolism and disposition of p-chloroaniline in rat, mouse, and monkey.the metabolism and disposition of [14c]p-chloroaniline was studied in the male fisher 344 rat, female c3h mouse, and male rhesus monkey. greater than 90% of the radiocarbon is eliminated through the urine in all species. a major route of metabolism is orthohydroxylation, whereby 2-amino-5-chlorophenyl sulfate is the major excretion product in all species. excretion of p-chloro-oxanilic acid and p-chloroglycolanilide, oxidation products of p-chloroacetanilide, is significant in the rat. the appea ...19911676638
the beneficial effect of dextran on anastomotic patency and flap survival in a strongly thrombogenic model.dextran lowers the probability of thrombus formation, by reducing platelet aggregation and adhesion and by increasing fibrinolysis. all studies to date using dextran for microvascular reconstruction have examined only short-term (1 to 2 hr) patency in isolated vessels. the current study used an established thrombotic model (inverted sleeve interposition graft), to investigate the effect of dextran on the long-term survival of pedicle flaps. a6- x 3-cm epigastric flap was elevated. a 2-mm inverte ...19921383537
regional brain monoamine levels and utilization in middle-aged rats.significant changes in monoamine levels and utilization were noted in certain brain regions of middle-aged fisher 344 rats when compared with young adult controls. in the prefrontal cortex and septum, 3,4 dihydroxyphenylglycol (mhpg) and the mhpg/norepinephrine (ne) ratio were decreased. the septum also showed increases in dopamine (da) and dihydroxyphenylacetic acid (dopac) but there was a decrease in the dopac/da ratio. the striatum showed an increase in the mphg/ne ratio and an increase in do ...19901689446
sequential appearance of intestinal mucosal cell types in the right and caudate liver lobes of furan-treated rats.furan rapidly induces in rat liver a unique, lobe-specific pattern of development of intestinal metaplasia and associated cholangiofibrosis. to establish early cell-precursor relationships in the genesis of this cholangiofibrosis, a time-course study was conducted in which young adult male fisher 344 rats received furan by gavage at a daily dose of 45 mg/kg body wt over a 32-day treatment period. an analysis of individual liver lobes obtained at different time points from these animals during fu ...19921427660
effects of 6-methylene progesterone on growth, morphology, and blood flow of the dunning r3327 prostatic adenocarcinoma.copenhagen x fisher f1 rats were implanted with the androgen-dependent dunning r3327 prostatic adenocarcinoma. when the tumors had median volumes of ca 470 mm3, the rats were castrated and/or treated with 6-methylene-4-pregnene-3,20-dione (6mp) in different doses. tumor growth inhibition occurred in all castrated and treated groups, with decrease in volume of the epithelial compartment in the intact group. tumor volumes at the highest dose level of 6mp equalled those observed in the castrate gro ...19921574466
human monoclonal antibodies to pseudomonas aeruginosa type-specific lipopolysaccharides, toxin a and klebsiella capsular polysaccharides.a number of human monoclonal antibodies (hmab) recognizing type-specific determinants expressed by the lipopolysaccharide (lps) of pseudomonas aeruginosa and by the capsular polysaccharide (cps) of klebsiella were generated for potential treatment of nosocomial infections. the goal is to administer these type-specific hmab prophylactically as a "cocktail" providing broad coverage. lymphoblastoid cell lines (lcl) secreting hmab recognizing p. aeruginosa lps, toxin a or klebsiella cps were obtaine ...19901698165
comparison of growth, neovascularization, and enzymatic function of fetal intestinal grafts in the omentum and renal capsule.fetal tissues are less immunogenic and may be a useful donor source for organ transplantation. this report compares the fate of fetal small bowel segments transplanted in the omentum and renal capsule of recipient syngeneic rats. two-centimeter segments of fetal jejunum and ileum were obtained from 26 donor 19-day gestational age rat fetuses and transplanted into the subrenal capsule (n = 35) and omentum (n = 40) in syngeneic fisher rats (weight, 150 g) as free grafts. no immunosuppression was u ...19901698219
characterization of flat revertants isolated from cells transformed by abelson murine leukemia virus (ab-mulv).transformed fisher rat fibroblast cell lines by abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. molecular biological analysis of three revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. these findin ...19901703165
identification of an epitope encoded in the env gene of friend murine leukemia virus recognized by anti-friend virus cytotoxic t lymphocytes.we have previously shown that strong epitopes recognized by anti-friend virus (fv) cytotoxic t lymphocytes (ctl) in h-2b mice are encoded in both the env and gag/pol regions of the helper friend leukemia virus genome. two approaches have been used to identify these epitopes. at the nucleic acid level, we have constructed env genes with either of two in-frame deletions: pkr2, an env gene with a 681-bp deletion in the gp70 region and inserted into the psv2-gpt-1 expression vector; and pkr1, an env ...19911704662
antibody-targeted photolysis. bacteriocidal effects of sn (iv) chlorin e6-dextran-monoclonal antibody conjugates.antibody-targeted photolysis is a technique for damaging or killing cells using light and an antibody-bound photosensitizer. in the present study, immunoconjugates were constructed to selectively kill pseudomonas aeruginosa bacteria using tin (iv) chlorin e6 which was linked to dextran and then bound to the carbohydrate moiety of a monoclonal antibody specific for pseudomonas aeruginosa fisher type i polysaccharide antigen. killing of pseudomonas during mid-log phase growth was shown to be depen ...19911706578
acute toxicity of trans-4-hydroxy-2-nonenal in fisher 344 rats [corrected].the potential toxicity of trans-4-hydroxy-2-nonenal (hne), a product formed in vivo during lipid peroxidation, which is also present in foods, was investigated in fisher 344 rats. five groups of five male rats each were given by gavage 1000, 300, 100, 30 or 10 mg/kg body weight hne dissolved in 0.5 ml corn oil. the sixth group, the control, received corn oil alone. two rats died 6 and 8 hr after being treated with 1000 mg/kg hne. these two rats showed extensive acute tubular necrosis of the kidn ...19921608305
tcdd causes stimulation of c-ras expression in the hepatic plasma membranes in vivo and in vitro.a series of in vivo and in vitro experiments were conducted to determine the effects of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (tcdd) administered on the expression of c-ras. differences in c-ras expression between control and tcdd treated groups were determined by immunoassay of p21ras protein, or indirectly measured by the specific binding of 3h-gtp to hepatic plasma membrane preparations. intraperitoneal injection of sublethal doses of tcdd significantly elevated (p less than 0.05, student t ...19921404241
identification of t cell epitopes within a 23-kd antigen (p24) of toxoplasma gondii.among the potentially vaccinating antigens, the products excreted/secreted by the parasite t. gondii have been demonstrated to be excellent candidates. the molecular cloning of one of these antigens (p24) present in excreted/secreted antigens (esa) has recently been carried out in our laboratory. the recombinant antigen p24 corresponds to a native molecule of 23 kd. we were interested in determining the main epitopes of the p24 antigen eliciting a t lymphocyte response using synthetic peptides d ...19911710549
the effects of chronic ethanol consumption on the mesolimbic and nigrostriatal dopamine systems.both the mesolimbic dopamine system, which is involved with the rewarding properties of several drugs of abuse, and the nigrostriatal dopamine system, which is involved with motor function, appear to be sensitive to the effects of ethanol. in order to determine which components of the mesolimbic and nigrostriatal dopamine systems are adversely affected by chronic ethanol consumption, we assessed dopamine and dopac (3,4-dihydroxyphenylacetic acid) concentration and d1 and d2 receptors in several ...19921534209
macromolecular adduction by trichloroacetonitrile in the fischer 344 rat following oral gavage.male fisher 344 rats were administered 1- or 2-[14c]trichloroacetonitrile (tcan) by oral gavage. dna was isolated from the liver, kidneys and stomach and several protein fractions (globin, albumin and globulins) were isolated from blood. tcan binds to both the dna and the blood proteins in a dose-related manner. more radiolabel was associated with the dna when the carbon at c2 position was labeled, than at c1 position. however, the position of the radiolabel did not influence the levels of radio ...19921540927
the jeanne manery-fisher memorial lecture 1991. maturation of reticulocytes: formation of exosomes as a mechanism for shedding membrane proteins.the transferrin receptor is a member of a group of reticulocyte surface proteins that disappear from the membranes of reticulocytes as the cells mature to the erythrocyte stage. the selective loss of membrane proteins appears to be preceded by the formation of multivesicular bodies (mvbs). at the reticulocyte stage, many species of mammalian red cells including man, and one nucleated avian species (chicken), contain these intracellular structures in both natural and induced anemias. also charact ...19921515120
modification of alkylating agent induced cell kill by 2-nitroimidazoles in unclamped and clamped sc 9l tumors.enhanced cell kill has been observed when experimental tumors were treated with alkylating agents in combination with 2-nitroimidazoles (2-ni). in this study, modification of the cell kill induced by cyclophosphamide (cy) and an analog, ifosfamide (ifo), by two radiation sensitizers, misonidazole (miso) and etanidazole (sr-2508), was measured. three important parameters were determined: (a) the necessity for hypoxic reduction of the 2-ni to achieve an increase in tumor cell kill, (b) the optimal ...19921531976
angiopolarity: a new design parameter for cell transplantation devices and its application to degradable systems.the purpose of this study was to obtain directional angiogenesis of small blood vessels and capillaries to an implant made from a resorbable polymer for hepatocyte transplantation. it was intended to mimic the native acinar structure of the liver in form to facilitate replication of the cells and organ growth. the implant device structure was designed for injection to minimize surgical trauma. hollow microspheres with an open porous wall structure and one large central opening were made from pol ...19911721514
a comparison of hiv-1 antibody classes, titers, and specificities in paired semen and blood samples from hiv-1 seropositive men.twenty-eight paired blood and semen samples obtained from human immunodeficiency virus type 1 (hiv-1) seropositive men at various stages of disease progression were evaluated for titer and immunoglobulin (ig) class by an enzyme-linked immunosorbent assay (elisa). blood antibody titers ranged from 40,000 to 4,000,000 with a median of 40,000. semen titers ranged from 400 to 40,000 with a median of 400. hiv-1 antibody titers in matched semen and blood samples showed a strong positive correlation (r ...19921738088
inhibitory effect of 4-methylpyrazole on antipyrine clearance in rats.pyrazole and 4-methylpyrazole (4-mp) are potent, effective inhibitors of alcohol dehydrogenase. pyrazole and its derivatives also have been shown to affect the cytochrome p-450 dependent monooxygenase system. this study was performed to investigate the effect of 4-mp on the disposition kinetics of antipyrine (ap). groups of male fisher 344 rats were given an ip injection of 4-mp (100 mg/kg) or 4-mp hcl (equivalent to 4-mp 100 mg/kg) or an equivalent volume of saline. ap (20 mg/kg) was injected i ...19921740974
age-related decrease in omega conotoxin binding to rat cardiac synaptosomes.a cardiac synaptosomal preparation developed by this laboratory was used to study neuronal calcium channels in aging rat heart. ca2+ channels were quantified by measuring binding of iodinated omega conotoxin, which is reported to specifically block neuronal ca2+ channels. we determined the binding of [125i]-omega conotoxin gvia to a synaptosomal preparation from the hearts of 6- and 24-month-old male fisher 344 rats. the maximum number of binding sites (bmax +/- sd, fmol/mg protein) is lower in ...19921549624
age and food restriction alter the porphyrin concentration and mrna levels for 5-aminolevulinate synthase in rat harderian gland.the effects of age and food restriction on the porphyrin concentration in harderian glands were studied in male fisher 344 rats. harderian gland porphyrin concentrations increased with age; this was statistically significant in 20 month old animals compared with 3 month old animals. food restriction (by 40%) prevented the age-associated rise in porphyrins; thus, in 20 month old food restricted rats had porphyrin concentrations similar to those found in young animals. in a second experiment, we c ...19921447953
[the early hemiparesis of stroke in evolution of basilar artery--case report].in basilar artery thrombosis, hemiparesis without obvious brainstem signs may precede the tetraplegia with coma or a locked-in state. this premonitory hemiparesis was called as "herald hemiparesis" by fisher (1988). its early detection is important because immediate anticoagulant therapy may prevent its evolution. we reported two patients with such hemiparesis. the first case was a 71-year-old diabetic and hypertensive man who was admitted because of right hemiparesis, dysarthria and decreased s ...19921562385
immune system of the spontaneously hypertensive rat. i. sympathetic innervation.extensive bidirectional interactions are believed to exist between the sympathetic nervous system and the immune system. the spontaneously hypertensive rat (shr) is known to possess both increased sympathetic nervous system activity with increased tissue catecholamine levels in several peripheral organs and a moderate t lymphocyte immune deficiency. we examined the development of innervation in both primary (thymus) and secondary (spleen) organs of the immune system of the shr compared to immuno ...19921618286
distribution of red blood cell velocity in capillary network, and endothelial ultrastructure, in aged rat skeletal muscle.although age-related structural and functional changes in skeletal muscle have been described extensively, little is known about the accompanying hemodynamic and structural changes in the microvasculature. the objective of this study was to use the extensor digitorum longus muscle in mid-aged (12 months) and old (28 months) fisher 344 male rats to evaluate (1) the distribution of microvascular flow in the resting state, (2) the distribution response to a complete 30-min tourniquet ischemia, and ...19921640874
[fresh allograft of intact growth plate into immature articular cartilage defects in rats].in three inbred rat strains with a distinct major histocompatibility complex (mhc), mhc-matched allografts (fisher, rt1l to lewis, rt1l, n = 39) or mhc-mismatched allografts (brown norway, rt1n to lewis, rt1l, n = 38) of fresh intact cartilage obtained from the tibial epiphyseal growth-plate were grafted into the defects in the femoral articular surface of immature rats. in the controls (n = 72), similar defect on the joint cartilage were made with no epiphyseal plate grafts. the present histolo ...19921506749
an evaluation of the sensitivity and specificity of medical thermography for the documentation of myofascial trigger points.this investigation evaluated the diagnostic value of medical thermology for the documentation of myofascial trigger points. previous investigators have suggested that circumscribed 'hot spots' reflect the thermal activity of trigger points. a total of 365 patients participated in the four separate experiments. upper back trigger points were isolated via palpation. a separate thermographic examination, specific to that experiment, was conducted by a technologist who was blind to the presence or a ...19921589239
syngeneic schwann cells derived from adult nerves seeded in semipermeable guidance channels enhance peripheral nerve regeneration.at present, clinical strategies to repair injured peripheral nerve concentrate on efforts to attain primary suture of the cut nerve ends. if this is not possible, autografts are used to unite the separated nerve segments. both strategies are based on the recognition that the schwann cells resident in the peripheral nerve trunk play a crucial role in the regenerative process. neither strategy may be feasible, however, in extensive or multiple injuries because the amount of autograft material is l ...19921527582
food restriction retards aging of the pineal gland.the effects of chronic (40%) food restriction from 6 weeks of age were studied in aging male fisher 344 rats. when compared with 3-month-old, ad libitum fed rats, pineal n-acetyltransferase (nat) activity had declined to less than 30% and pineal and serum levels of melatonin to 40% after 28 months when feeding had been ad libitum. food restriction significantly retarded this development (p less than 0.05) giving nat and melatonin levels which were twice as high as in the ad libitum fed group. ni ...19911713529
glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure.we have recently described the identification, isolation, and characterization of a factor, termed stem cell factor (scf), which acts on primitive hematopoietic progenitors of the marrow. a soluble form of the factor was isolated from the conditioned medium of a rat cell line (zsebo, k. m., wypych, j., mcniece, i. k., lu, h. s., smith, k. a., karkare, s. b., sachdev, r. k., yuschenkoff, v. n., birkett, n. c., williams, l. r., satyagal, v. n., tung, w., bosselman, r. a., mendiaz, e. a., and langl ...19911717457
comparison of liver glycosylasparaginases from six vertebrates.structural and physical properties of glycosylasparaginase (ec 3.5.1.26) from the livers of human, pig, cow, rat, mouse and chicken were compared. the enzyme in all species had a common basic structure of two n-glycosylated subunits of about 24 (alpha) and 20 (beta) kda joined by non-covalent forces. subunit-specific antisera against the rat glycosylasparaginase bound specifically and sensitively to the corresponding subunits from all species. identity of 80% of the amino acids was found between ...19921554372
chlorophyllin, an antimutagen, acts as a tumor promoter in the rat-dimethylhydrazine colon carcinogenesis model.chlorophyllin (chl), the water soluble sodium/copper salt of chlorophyll, was investigated for its effect on colorectal cancer risk in the rat-dimethyldrazine colon carcinogenesis model. ninety weanling fisher 344 male rats were treated with five weekly injections of 1,2 dimethylhydrazine (dmh), 20 mg base/kg body weight. rats had been previously divided into three groups, consuming either rat chow and water (group i), rat chow and chl 1.5 mm in water throughout the experiment (group ii), or wat ...19921622132
pharmacodynamic and protective properties of a murine lipopolysaccharide-specific monoclonal antibody in experimental pseudomonas aeruginosa pneumonia in mice.we employed a pseudomonas aeruginosa mouse pneumonia model to evaluate the ability of a murine monoclonal antibody (mab) specific for the o-side chain of p. aeruginosa fisher immunotype-1 lipopolysaccharide (lps) to achieve and sustain therapeutic levels in plasma and lung tissue, reduce bacterial populations in the lung, and prevent pneumonia-associated mortality. an igg3 mab (y1-5a4) administered to mice i.v. over a dose range of 125-1,000 micrograms/mouse produced plasma and lung tissue level ...19911808464
estrogen increases galanin immunoreactivity in hyperplastic prolactin-secreting cells in fisher 344 rats.galanin is a widely distributed regulatory peptide which modulates the pituitary secretion of prl and gh. estrogen administration strongly stimulates galanin gene expression in the rat anterior pituitary. in adult female fischer 344 rats, estrogen also induces hyperplasia of lactotropes. we used immunocytochemical analysis to assess the effects of estrogen on galanin-like immunoreactivity (gal-ir) in the rat pituitary and hypothalamus during sc diethylstilbestrol (des) implantation and after its ...19901693569
rearrangement of chromosome 1p in breast cancer correlates with poor prognostic features.in a cytogenetic study of breast cancer biopsies, clonal abnormalities of chromosome 1p were identified in 56% (14) of 25 informative patients. translocations predominated, involving 1p22 (n = 1), 1p35 (n = 1) or 1p36 (n = 10) breakpoints. chromosome 1p abnormalities were associated with estrogen receptor (er) negativity (p = 0.03, 2-tailed fisher exact probability test), high histological grade (p = 0.02, 2-tailed mann-whitney u-test) and an unfavourable melbourne prognostic score (nepa p = 0.0 ...19921637662
factor xiii of blood coagulation inhibits the oxidative phagocyte metabolism and suppresses the immune response in vivo.factor xiii of blood coagulation (f xiii) belongs to the family of transglutaminases and is a major cell product of certain subsets of macrophages. the gene for f xiiia is coupled to the immune response genes of the hla-region on chromosome 6. f xiii dose- dependently inhibits the in vitro chemiluminescence response of human phagocytes. about 0.1 units of f xiii/ml (final) decreased the chemiluminescence response to about 50%. in addition, about 0.6 units of f xiii/ml inhibits 50% of the release ...19911771628
spontaneous transformation of rat ovarian surface epithelial cells: association with cytogenetic changes and implications of repeated ovulation in the etiology of ovarian cancer.ovarian surface epithelial cells undergo several rounds of division to repair the wound created by follicular rupture at the time of ovulation. this cyclical requirement for cell division, when not interrupted by the long anovulatory rest periods that occur during pregnancy and lactation, may contribute to the development of ovarian cancer.19921556770
response of serum zinc and copper to inflammatory stimulation in the rat with chronic liver damage.to measure the seric levels of zinc and copper in healthy and in hepatic damaged rats when an inflammatory stimulus is applied.19911844115
strain differences in expression of virulence by the 90 kilobase pair virulence plasmid of salmonella serovar typhimurium.a number of plasmidless strains were obtained by curing the 90 kilobase pair (kb) virulence plasmid from six strains, c5, tml, w118, sr11, lt2 and fisher, of salmonella serovar typhimurium. a number of transposon (tn5) tagged 90 kb plasmids, also derived from these typhimurium strains, were then transferred back into these plasmidless strains. plasmid-cured strains, reconstituted strains, and the parental strains were tested for their virulence in balb/c mice. there were two groups of typhimuriu ...19911654496
purification and properties of the h(+)-nicotinamide nucleotide transhydrogenase from rhodobacter capsulatus.1. h(+)-transhydrogenase from rhodobacter capsulatus is an integral membrane protein which, unlike the enzyme from rhodospirillum rubrum, does not require the presence of a water-soluble component for activity. 2. the enzyme from rb. capsulatus was solubilised in triton x-100 and subjected to ion-exchange, hydroxyapatite and then gel-exclusion column chromatography. sds/page of the purified enzyme revealed the presence of two polypeptides with apparent mr 53,000 and 48,000. other minor component ...19911849819
transport of peptides in renal brush border membrane vesicles. suitability of 125i-labelled tyrosyl peptides as substrates.three tyrosine-containing peptides (tyr-pro, tyr-pro-phe and tyr-pro-phe-pro) were [125i]iodo-labelled and their uptake characteristics in renal brush border membrane vesicles was investigated to assess the suitability of these peptides as substrates in peptide transport studies. hydrolysis of these peptides during uptake measurements was avoided by using brush border membrane vesicles prepared from the kidneys of japan fisher 344 rats which genetically lack dipeptidylpeptidase iv activity. the ...19911681904
actions of phosphomonoesters on ca1 hippocampal neurons as revealed by a combined electrophysiological and nuclear magnetic resonance study.phosphomonoesters (pmes), precursors of membrane phospholipids, are found in high levels in the developing brain and alzheimer's disease brain. the present study details the neurophysiological and metabolic effects of acute pme elevation on the fisher 344 rat in vitro hippocampal slice. two abundant pmes, phosphoethanolamine (pe) and l-phosphoserine (ps), reliably altered properties of synaptic transmission at the schaffer collateral/commissural-ca1 cell synapse. specifically, pe reversibly depr ...19911796353
a study of the ability of an in situ remineralization model to differentiate between the effects of two fluoride dentifrices that produced significantly different clinical caries results.the purpose of this study was to determine whether an in situ remineralization model was able to show a difference in the effects of two dentifrices of different fluoride concentrations and significantly different clinical efficacies. three dentifrices were tested in a double-blind, cross-over study design. the products contained 0 ppm f, 250 ppm f, or 1000 ppm f from sodium fluoride and were formulated with a silica base according to the formulations used in a human caries trial (koch et al., 1 ...19921607435
enhancement of antitumor drug cytotoxicity via laser photoactivation.we investigate the efficacy of daunomycin, some imino- and amino-substituted daunomycin analogues and the disubstituted aminoanthracenedione, mitoxantrone, in photosensitizing short-term cell kill upon irradiation in the long wavelength visible range, during incubation of fisher rat thyroid cells with the drugs. while all compounds exhibit similar cytocidal effects on our cell line, in the absence of irradiation, administering 86 j/cm2 at wavelengths either coincident or close to drug absorption ...19911886938
t cell reactivity to an epitope of the mycobacterial 65-kda heat-shock protein (hsp 65) corresponds with arthritis susceptibility in rats and is regulated by hsp 65-specific cellular responses.adjuvant arthritis (aa) can be induced in genetically susceptible rats by immunization with heat-killed mycobacteria suspended in mineral oil. from our analysis of arthritogenic t cell clone a2b, obtained from an arthritic lewis rat and specific for the 180-188 epitope of mycobacterial 65-kda heat-shock protein (hsp 65), the possible origin of aa was explained by the existence of a molecular mimicry of the 180-188 epitope with a cartilage-associated self antigen. we now have shown that lewis rat ...19911709871
does exercise stress alter susceptibility to bacterial infections?swimming was used for evaluating alterations in performance capacity and as a means for studying the influence of exercise stress on susceptibility to streptococcus pneumoniae and francisella tularensis infections in two strains of rats, i.e. fisher-dunning (fd) and sprague-dawley (sd). the performance capacity was reduced by both diseases and was correlated to the dose of the given micro-organism. fd rats, however, were more susceptible to the infection and showed a greater deterioration than s ...19911897063
the role of cytophilic igg3 antibody in t cell-mediated resistance to infection with the extracellular bacterium, pseudomonas aeruginosa.previous studies have demonstrated that t lymphocytes from mice immunized with a high m.w. polysaccharide ag from fisher-devlin immunotype i pseudomonas aeruginosa can adoptively transfer protection against challenge with the homologous bacterial strain to susceptible mice. this t cell-mediated resistance has been found to be b cell dependent, although serum from immunized mice is incapable of passively transferring protection to nonimmune mice. the current studies demonstrate that t cells from ...19911898604
inhaled particle retention in rats receiving low exposures of diesel exhaust.to study the effects of a low concentration exposure on the retention and clearance of submicron particles from the lungs, we exposed male fisher 344 rats to diesel exhaust diluted to 50 micrograms diesel exhaust particles (dp)/m3, 20 h/d, 7 d/wk for 52 wk. lung burdens (amount of dp in lungs) and the alveolar macrophage burdens were measured up to 52 wk postexposure. by 1 yr postexposure at least 80% of the dp was eliminated from the lungs and similarly cleared from the lavaged pool of macropha ...19901691304
growth characteristics and metastatic potential of seven intestinal carcinoma lines serially passaged in syngeneic rats.transplantable tumour lines were obtained from one duodenal carcinoma induced by n-methyl-n'-nitro-n-nitrosoguanidine in the lewis rat and from six colonic carcinomas induced by 1,2 dimethylhydrazine in bdix or fisher rats. the tumours were serially transplanted by the subcutaneous route into homologous syngeneic rats. the seven tumours differ from one another in their histological structure, five of them being well or moderately differentiated adenocarcinomas, and in their capacity to produce n ...19911900965
structural characterization of the lipid a component of pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.the structure of the lipid a component of lipopolysaccharides isolated from two wild-type strains (fisher 2 and 7) and one rough mutant (pac 605) of pseudomonas aeruginosa was investigated using chemical analysis, methylation analysis, combined gas-liquid chromatography/mass spectrometry, laser-desorption mass spectrometry and nmr spectroscopy. the lipid a backbone was found to consist of a pyranosidic beta 1,6-linked d-glucosamine disaccharide [beta-d-glcpn-(1----6)-d-glcpn], phosphorylated in ...19911904818
importance of administration method in high-dose anticancer chemotherapy from toxicological standpoint in rats.we examined death rates in fisher rats when chemotherapeutic drugs were administered in a prescribed amount by either a single or divided dose. lower death rates were observed when nimustine hydrochloride (acnu) was given in a divided dose, or when cyclophosphamide (cy), vindesine sulfate (vds) or vincristine (vcr) was administered as a single dose. lower hematological toxicity was observed when acnu was administered in 2 doses and cy in a single dose. with etoposide, we did not observe any sign ...19911720885
functional enhancement of intrastriatal dopamine-containing grafts by the co-transplantation of sciatic nerve tissue in 6-hydroxydopamine-lesioned rats.peripheral nerve "bridges" demonstrate the ability to facilitate axonal growth and regenerate adult and fetal central nervous system tissue. the purpose of this study was to determine if co-grafted peripheral nerve tissue could enhance the ability of fetal dopamine (da) cell transplants to reinnervate host striatum that had been denervated unilaterally. male fisher-344 rats were unilaterally lesioned with 6-hydroxydopamine to eliminate the nigrostriatal da pathway. a total of 31 rats demonstrate ...19911907926
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