| descriptions of prevotella tannerae sp. nov. and prevotella enoeca sp. nov. from the human gingival crevice and emendation of the description of prevotella zoogleoformans. | prevotella tannerae sp. nov. and prevotella enoeca sp. nov. from the human gingival crevice are described. these organisms are obligately anaerobic, non-spore-forming, nonmotile, gram-negative, rod-shaped bacteria that ferment carbohydrates and produce succinic and acetic acids. bile inhibits growth. some strains (38%) of p. tannerae produce colonies with a tan to black pigment when they are grown on rabbit blood agar. the type strains are p. tannerae atcc 51259 and p. enoeca atcc 51261. in addi ... | 1994 | 7981091 |
| isolation and identification of prevotella tannerae from endodontic infections. | black-pigmented bacteria are often isolated from endodontic infections. five strains of black-pigmented bacteria isolated from endodontic infections could not be identified in our laboratory. the purpose of this study was to sequence the 16s rrna gene of the five unknown isolates and identify the organisms. the 16s rrna genes from the unknown organisms were cloned, sequenced, and determined to be prevotella tannerae. in addition, samples from endodontic infections were surveyed for the presence ... | 2000 | 11154415 |
| changes in predominant bacterial populations in human faeces with age and with clostridium difficile infection. | the bacterial composition of human faeces can vary greatly with factors such as age and disease, although relatively few studies have monitored these events, particularly at species level. in this investigation, bacteria were isolated from faecal samples from healthy young adults and elderly subjects, and elderly patients with clostridium difficile-associated diarrhoea (cdad). the organisms were identified to species level on the basis of their cellular fatty acid profiles with the midi system. ... | 2002 | 11990498 |
| pcr methodology as a valuable tool for identification of endodontic pathogens. | this paper reviews the principles of polymerase chain reaction (pcr) methodology, its application in identification of endodontic pathogens and the perspectives regarding the knowledge to be reached with the use of this highly sensitive, specific and accurate methodology as a microbial identification test. | 2003 | 12799118 |
| geographical differences in bacteria detected in endodontic infections using polymerase chain reaction. | the polymerase chain reaction (pcr) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. the purpose of this study was to use pcr to detect the presence of specific species of bacteria in samples collected from two geographical locations. microbial samples from abscesses of endodontic origin were collected from patients in portland, oregon, and rio de janeiro, brazil. pcrs with species-specific oligonucleotide ... | 2004 | 15055430 |
| a new checkerboard panel for testing bacterial markers in periodontal disease. | various microbiological methods have been used for testing bacterial markers for periodontitis and periodontal disease progression. most studies have used only a limited number of well recognized bacterial species. the purpose of the present study was to evaluate the association of 13 more recently identified bacterial species in a new panel in comparison with 12 previously more recognized periodontotopathogens ('old panel') using the 'checkerboard' dna-dna hybridization method. | 2006 | 16390335 |
| anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor. | the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ... | 2003 | 16887710 |
| use of multiple-displacement amplification and checkerboard dna-dna hybridization to examine the microbiota of endodontic infections. | multiple-displacement amplification (mda) has been used to uniformly amplify bacterial genomes present in small samples, providing abundant targets for molecular analysis. the purpose of this investigation was to combine mda and checkerboard dna-dna hybridization to examine the microbiota of endodontic infections. sixty-six samples were collected from teeth with endodontic infections. nonamplified and amplified samples were analyzed by checkerboard dna-dna hybridization for levels and proportion ... | 2007 | 17634304 |
| microbiota of deciduous endodontic infections analysed by mda and checkerboard dna-dna hybridization. | to evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard dna-dna hybridization after uniform amplification of dna in samples by multiple displacement amplification (mda). | 2010 | 21083570 |