| etiologic agents and diseases found associated with clinical aspergillosis in falcons. | the aim of this study was to describe parasitological, microbiological, and pathological findings associated with the isolation of aspergillus species in 94?clinically diseased captive falcons from dubai. concomitant agents and/or diseases were identified in 64?cases, causing either single (n = 36) or multiple coinfections (n = 28). diagnoses found more often in association with aspergillosis were chronic fatigue and immune dysfunction syndrome (cfids) (n = 29), caryospora sp. (n = 16), serratos ... | 2011 | 21754937 |
| a multivalent mannheimia/bibersteinia vaccine protects bighorn sheep against mannheimia haemolytica challenge. | bighorn sheep (bhs) are more susceptible than domestic sheep (ds) to mannheimia haemolytica pneumonia. although both species carry m. haemolytica as a commensal bacterium in the nasopharynx, ds carry mostly leukotoxin (lkt)-positive strains while bhs carry lkt-negative strains. consequently, antibodies to surface antigens and lkt are present at much higher titers in ds, but lower in bhs. the objective of this study was to determine whether repeated immunization of bhs with multivalent mannheimia ... | 2011 | 21832104 |
| incorporation of antigens from mannheimia haemolytica culture supernatant, and recombinant bovine c3d into iscom matrix using neutravidin-biotin interaction. | the aim of this study was to incorporate antigens from mannheimia haemolytica culture supernatant, and an immune modulatory molecule, recombinant bovine c3d (rboc3d), into immune stimulating complexes (iscoms) using neutravidin-biotin interaction. biotinylated iscom matrix was generated using a commercial kit. the biotinylated iscom matrix was incubated with neutravidin and then centrifuged in a sucrose density gradient. the rboc3d was expressed as an in vivo biotinylated protein and with a c-my ... | 2011 | 21679244 |
| prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. | the prevalence of respiratory pathogens in diseased veal calves was determined in 24 respiratory disease outbreaks in 15 herds in belgium. bacteria were cultured from nasopharyngeal swabs and seroconversion against viruses and mycoplasma bovis was determined on paired sera. at the individual calf level, mycoplasma species, mannheimia haemolytica and pasteurella multocida, were isolated from 70.5 per cent, 21.5 per cent and 26.0 per cent of swabs, respectively. at the herd level, the presence of ... | 2011 | 21831999 |
| cytokine expression by pulmonary leukocytes from calves challenged with wild-type and leukotoxin-deficient mannheimia haemolytica. | the objective of this study was to assess the role of leukotoxin (lkt) in modulating the pulmonary cytokine response of calves challenged with mannheimia haemolytica. thirty-six calves, seronegative to lkt and m. haemolytica whole cell antigen were divided into three groups (i, ii and iii). calves in groups i and ii were challenged by the intra-bronchial route with 25ml of phosphate buffered saline (pbs) containing 0.44+ù10(9)cfu/ml of lkt deficient (lkt(-)) and 25ml of pbs containing 0.31+ù10(9 ... | 2011 | 21696986 |
| combinations of macrolide resistance determinants in field isolates of mannheimia haemolytica and pasteurella multocida. | respiratory tract infections in cattle are commonly associated with the bacterial pathogens mannheimia haemolytica and pasteurella multocida. these infections can generally be successfully treated in the field with one of several groups of antibiotics, including macrolides. a few recent isolates of these species exhibit resistance to veterinary macrolides with phenotypes that fall into three distinct classes. the first class has type i macrolide, lincosamide, and streptogramin b antibiotic resis ... | 2011 | 21709086 |
| molecular signatures (conserved indels) in protein sequences that are specific for the order pasteurellales and distinguish two of its main clades. | the members of the order pasteurellales are currently distinguished primarily on the basis of their branching in the rrna trees and no convincing biochemical or molecular markers are known that distinguish them from all other bacteria. the genome sequences for 20 pasteurellaceae species/strains are now publicly available. we report here detailed analyses of protein sequences from these genomes to identify conserved signature indels (csis) that are specific for either all pasteurellales or its ma ... | 2011 | 21830122 |
| relationship of in vitro minimum inhibitory concentrations of tilmicosin against mannheimia haemolytica and pasteurella multocida and in vivo tilmicosin treatment outcome among calves with signs of bovine respiratory disease. | objective-to determine associations between in vitro minimum inhibitory concentrations (mics) of tilmicosin against mannheimia haemolytica and pasteurella multocida and in vivo tilmicosin treatment outcome among calves with clinical signs of bovine respiratory disease (brd). design-observational, retrospective, cohort study. animals-976 feeder calves with clinical signs of brd enrolled in 16 randomized clinical trials. procedures-records of clinical trials from october 26, 1996, to november 15, ... | 2011 | 21718206 |
| investigating the candidacy of lipopolysaccharide-based glycoconjugates as vaccines to combat mannheimia haemolytica. | inner core lipopolysaccharide (lps) has been shown to be conserved in the majority of veterinary strains from the species mannheimia haemolytica, actinobacillus pleuropneumoniae and pasteurella multocida and as such is being considered as a possible vaccine antigen. the proof-in-principle that a lps-based antigen could be considered as a vaccine candidate has been demonstrated from studies with monoclonal antibodies raised to the inner core lps of mannheimia haemolytica, which were shown to be b ... | 2011 | 21701793 |
| defective bacterial clearance is responsible for the enhanced lung pathology characteristic of mannheimia haemolytica pneumonia in bighorn sheep. | the molecular and cellular basis for the enhanced lung pathology and mortality caused by mannheimia haemolytica in bighorn sheep (bhs, ovis canadenesis), in comparison to domestic sheep (ds, ovis aries), is not clear. polymorphonuclear leukocytes (pmns) of bhs are four- to eight-fold more susceptible to m. haemolytica leukotoxin-induced cytolysis, which is likely to reduce the number of functional phagocytes in the lung. we hypothesized that enhanced lung pathology is due to defective clearance ... | 2011 | 21742446 |
| Capsular polysaccharide vaccine for Group B Neisseria meningitidis, Escherichia coli K1, and Pasteurella haemolytica A2. | We reviewed the literature that is the basis for our proposal that (2?8)-a-Neu5Ac conjugates will be safe and effective vaccines for Group B meningococci (GBMs), Escherichia coli K1, and Pasteurella haemolytica A2. Although (2?8)-a-Neu5Ac is a virulence factor and a protective antigen of these three pathogens, it is also a component of normal tissues (neural cell adhesion molecule). Natural, anti-(2?8)-a-Neu5Ac present in most adults, vaccine-induced antibodies, and even high levels of spontaneo ... | 2011 | 22025709 |
| Immunogenicity of Mannheimia haemolytica Recombinant Outer Membrane Proteins Serotype 1-Specific Antigen, OmpA, OmpP2, and OmpD15. | We previously identified Mannheimia haemolytica outer membrane proteins (OMPs) that may be important immunogens by using immunoproteomic analyses. Genes for serotype 1-specific antigen (SSA-1), OmpA, OmpP2, and OmpD15 were cloned and expressed, and recombinant proteins were purified. Objective 1 of this study was to demonstrate immunogenicity of the four recombinant OMPs in mice and cattle. Objective 2 was to determine if the addition of individual recombinant OMPs or combinations of them would ... | 2011 | 21976226 |
| a snap-shot of mannheimia hemolytica a1 gene expression during infection in the bovine host. | it is expected that mannheimia hemolytica a1 expresses a particular collection of genes during infection in the host. the bacterial gene products are produced in the in vivo environment to facilitate growth and survival. here, we examined gene expression by m. hemolytica a1 in the bovine host after 6 days of infection. total rna from m. hemolytica a1 recovered from pneumonic lungs of two animals was used to produce cdna to screen a custom m. hemolytica a1 microarray. the expression profile was c ... | 2011 | 22029794 |
| Epidemiology of Mycoplasma bovis in Pennsylvania veal calves. | The objective of the study was to estimate the prevalence and incidence of Mycoplasma bovis, a common cause of pneumonia, in veal calves. Using simple random sampling, 252 calves from 4 veal herds located in central Pennsylvania were selected and longitudinally followed for monthly collection of nasal swabs. Bronchial swabs and lung lesions were collected at the slaughterhouse. Nasal, bronchial, and lung lesion swabs were cultured for bacterial respiratory pathogens. Ninety lung lesions were ide ... | 2012 | 22192204 |
| identification and immunogenicity of mannheimia haemolytica s1 outer membrane lipoprotein plpf. | immunity against mannheimia haemolytica requires antibodies against leukotoxin (lkt) and bacterial cell surface antigens, most likely immunogenic outer membrane proteins (omps). five immunogenic outer membrane lipoproteins identified and characterized in m. haemolytica were designated pasteurella lipoproteins (plp) a, -b, -c, -d and -e. using immunoproteomics, we identified a heretofore-uncharacterized m. haemolytica immunogenic outer membrane lipoprotein that we designated plpf, which was previ ... | 2011 | 21875637 |
| Pathology in practice. Mannheimia haemolytica. | | 2011 | 22087719 |
| Distinct Roles of ß-Galactosidase Paralogues of the Rumen Bacterium Mannheimia succiniciproducens. | Mannheimia succiniciproducens, a rumen bacterium belonging to the family Pasteurellaceae, has two putative ß-galactosidase genes, bgaA and bgaB, encoding polypeptides whose deduced amino acid sequences share 56% identity with each other and show approximately 30% identity to the Escherichia coli gene for LacZ. The M. succiniciproducens bgaA (MsbgaA) gene-deletion mutant was not able to grow on lactose as the sole carbon source, suggesting its essential role in lactose metabolism, whereas the Msb ... | 2012 | 22081396 |
| ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer. | Background Integrative and conjugative elements (ICEs) have not been detected in Pasteurella multocida. In this study the multiresistance ICEPmu1 from bovine P. multocida was analysed for its core genes and its ability to conjugatively transfer into strains of the same and different genera. Methods ICEPmu1 was identified during whole genome sequencing. Coding sequences were predicted by bioinformatic tools and manually curated using the annotation software ERGO. Conjugation into P. multocida, M ... | 2012 | 22001176 |
| antimicrobial susceptibility patterns and sensitivity to tulathromycin in goat respiratory bacterial isolates. | bacterial pneumonia is a common and often life-threatening respiratory problem in both meat and dairy goats. options for approved antibiotic therapy in goats to combat these bacterial infections are severely limited and frequently drugs must be used in an extra-label manner. tulathromycin, a triamilide macrolide antimicrobial drug shown to be effective against swine and cattle respiratory bacterial agents, has been identified as a potentially useful drug in caprines. the present study was conduc ... | 2011 | 22078276 |
| bovine respiratory disease: efficacy of different prophylactic treatments in veal calves and antimicrobial resistance of isolated pasteurellaceae. | the present study was conducted to evaluate the efficacy of two prophylactic antibiotic treatments against bovine respiratory disease (brd) in veal calves. in addition, the antibiotic susceptibilities of isolated pasteurellaceae were tested. the calves were treated either on the day of arrival by a single administration of tulathromycin (group a, n=20), by a peroral administration of chlortetracycline, sulphadimidine, and tylosin (group b, n=20) for seven consecutive days, or were not prophylact ... | 2011 | 21945813 |
| impact of isoflupredone acetate treatment on clinical signs and weight gain in weanling heifers with experimentally induced mannheimia haemolytica bronchopneumonia. | objective-to characterize the impact of mannheimia haemolytica infection on feed intake and weight gain in feedlot heifers and to evaluate the clinical efficacy of isoflupredone acetate administered in combination with oxytetracycline. animals-96 weanling heifers in a research feedlot facility. procedures-bronchopneumonia was induced by intrabronchial infusion of m haemolytica. control heifers underwent a sham procedure. infected heifers were treated with oxytetracycline alone or in combinat ... | 2011 | 22126689 |
| serologic response to mannheimia haemolytica in calves concurrently inoculated with inactivated or modified-live preparations of m. haemolytica and viral combination vaccines containing modified-live bovine herpesvirus type 1. | to assess the serologic response of calves to inactivated and modified-live (ml) mannheimia haemolytica (mh) preparations given alone and concurrently with combination viral vaccines containing ml bovine herpesvirus type 1 (bhv-1). | 2011 | 22023134 |
| plasmid-mediated florfenicol resistance in mannheimia haemolytica isolated from cattle. | the aim of this study was to analyse a florfenicol-resistant mannheimia haemolytica isolated from a calf to determine the genetic basis of its florfenicol-resistance. the antimicrobial susceptibility and plasmid content of the isolate were determined. a florfenicol resistant plasmid carrying the flor gene was identified by pcr and transformed into escherichia coli jm109 and hb101 strains. the plasmid was then mapped and sequenced completely. the isolate was resistant to chloramphenicol, florfeni ... | 2011 | 22019290 |
| outer membrane protein a of bovine and ovine isolates of mannheimia haemolytica is surface exposed and contains host species-specific epitopes. | mannheimia haemolytica is the etiological agent of pneumonic pasteurellosis of cattle and sheep; two different ompa subclasses, ompa1 and ompa2, are associated with bovine and ovine isolates, respectively. these proteins differ at the distal ends of four external loops, are involved in adherence, and are likely to play important roles in host adaptation. m. haemolytica is surrounded by a polysaccharide capsule, and the degree of ompa surface exposure is unknown. to investigate surface exposure a ... | 2011 | 21896777 |
| Colostrum from cattle immunized with a vaccine based on iron regulated proteins of Mannheimia haemolytica confers partial protection. | Passive protection afforded by colostrum from cattle vaccinated prepartum with an inactivated combination vaccine against viral pathogens and Mannheimia haemolytica (M. haemolytica) was evaluated against an experimental M. haemolytica challenge. Newborn calves were either fed colostrum from vaccinated dams or control colostrum. At approximately 3 weeks of age 24 calves were experimentally infected with M. haemolytica. Animals of both groups displayed clinical signs of respiratory disease and lun ... | 2011 | 22154882 |
| Dominant expression of interleukin-8 vs interleukin-1ß and tumour necrosis factor alpha in lungs of lambs experimentally infected with Mannheimia haemolytica. | To quantify the number of cells infected with Mannheimia haemolytica and expressing interleukin (IL)-1ß, tumour necrosis factor alpha (TNFa) and IL-8 using immunohistochemistry, and to measure the immunoreactivity of cytokines in pulmonary tissue extracts using ELISA, in the lung of lambs experimentally infected with M. haemolytica, and to compare the patterns of expression of cytokines in airways at different times post-infection (p.i.). | 2011 | 21851299 |
| Survey of marbofloxacin susceptibility of bacteria isolated from cattle with respiratory disease and mastitis in Europe. | A monitoring programme conducted in Europe since 1994 to survey the marbofloxacin susceptibility of bacterial pathogens isolated from cattle has established the susceptibility of bacterial strains isolated before any antibiotic treatment from bovine mastitis and bovine respiratory disease (BRD) cases between 2002 and 2008. Minimum inhibitory concentration (MIC) was determined by a standardised microdilution technique. For respiratory pathogens, Pasteurella multocida and Mannheimia haemolytica is ... | 2011 | 22121154 |
| Pharmacokinetic and pharmacodynamic testing of marbofloxacin administered as a single injection for the treatment of bovine respiratory disease. | Vallé, M., Schneider, M., Galland, D., Giboin, H., Woehrlé, F. Pharmacokinetic and pharmacodynamic testing of marbofloxacin administered as a single injection for the treatment of bovine respiratory disease. J. vet. Pharmacol. Therap. doi: 10.1111/j.1365-2885.2011.01350.x. New approaches in Pharmacokinetic/Pharmacodynamic (PK/PD) integration suggested that marbofloxacin, a fluoroquinolone already licensed for the treatment of bovine respiratory disease at a daily dosage of 2 mg/kg for 3-5 days, ... | 2011 | 22126438 |
| efficacy and safety of a new 450 mg/ml florfenicol formulation administered intramuscularly in the treatment of bacterial bovine respiratory disease. | the objective of the study was the safety and efficacy evaluation of a new 450 mg/ml florfenicol formulation in the treatment of naturally occurring respiratory disease when administered intramuscularly, compared with a positive control group treated with the well-established 300 mg/ml formulation. a total of 174 calves, selected from five sites in france and spain, aged from 1 to 17 months, showing severe signs of respiratory disease, were randomly assigned to treatment with either the 300 mg/m ... | 2011 | 21921060 |
| [pilot study: serological research of bovine respiratory syncytial virus and mannheimia haemolytica in young cattle at dairy farms in the netherlands]. | | 2011 | 21870575 |
| a proteomic analysis of the regulon of the narp two-component regulatory system response regulator in the bovine pathogen mannheimia haemolytica a1. | abstract: | 2011 | 22114901 |
| aerobic oral and rectal bacteria of free-ranging steller sea lion pups and juveniles (eumetopias jubatus) in alaska. | bacteriologic cultures from oral, rectal, and lesion samples from free-ranging steller sea lion (ssl, eumetopias jubatus) pups and juveniles in alaska (2001-2005) were examined to determine frequency of infection by a specific subset of common and pathogenic aerobic bacteria. associations between isolated bacteria and age, sex, body condition, location, and sampling season were investigated. salmonella spp. isolates were further evaluated to determine spatial clustering (n=48) and to identify se ... | 2011 | 22102651 |