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deoxymulundocandin--a new echinocandin type antifungal antibiotic.a new echinocandin type antifungal antibiotic, deoxymulundocandin, c48h77n7o15, was isolated from the culture filtrate and mycelia of a fungal culture, aspergillus sydowii (bainier and sartory) thom and church var. nov. mulundensis roy (culture no. y-30462). the structure was established by comparative gc-ms analyses of the derivatized acid hydrolysates of deoxymulundocandin and mulundocandin as well as by the high field nmr experiments (cosy, noesy and dept).19921624363
water relations of paecilomyces variotii, eurotium amstelodami, aspergillus candidus and aspergillus sydowii, xerophilic fungi isolated from indonesian dried fish.the water relations of four xerotolerant fungi, paecilomyces variotii, eurotium amstelodami, aspergillus candidus and aspergillus sydowii, isolated from dried salt fish, were examined at 25 degrees c, on media in which water activity (aw) was controlled by nacl or a glucose/fructose mixture. all fungi were less tolerant of nacl than glucose/fructose at low aw. p. variotii grew 2 to 3 times faster on glucose/fructose media than on nacl. the minimum aw permitting germination varied from 0.753 for ...19883275312
mulundocandin, a new lipopeptide antibiotic. i. taxonomy, fermentation, isolation and characterization.mulundocandin, a new lipopeptide antibiotic, was isolated from the culture broth of a strain of aspergillus sydowi no. y-30462. the antibiotic, obtained as a colorless amorphous powder having the molecular formula c48h77n7o16, is an antifungal antibiotic active against yeasts and filamentous fungi.19873570979
enzymatic synthesis of novel fructosyl and oligofructosyl trehaloses by aspergillus sydowi beta-fructofuranosidase.an intracellular beta-d-fructofuranosidase produced by aspergillus sydowi iam 2544 was purified by q-sepharose and alkyl-sepharose chromatographies. the molecular mass was 50 kda by sds-page analysis. the optimum ph and temperature of sucrose hydrolyzing activity of the enzyme were 5.5 and 75 degrees c, respectively, but those of fructosyl transferase activity were 5.2 and 55 degrees c, respectively. the enzyme efficiently transferred the fructose residue of sucrose as a donor to trehalose as an ...19957766019
purification and some properties of a xylanase from aspergillus sydowii mg49.aspergillus sydowii mg49 produces a 30-kda exosplitting xylobiohydrolase during growth on xylan. a specific chemical modification and substrate protection analysis of purified xylanase provided evidence that tryptophan and carboxy and amino groups are present at the catalytic site of this enzyme. thermal inactivation of the xylanase occurs because of irreversible polymolecular aggregation, which is slower in the presence of glycerol.19947811101
a rejoinder to the commentary on 'thermostability of b-xylosidase from aspergillus sydowii mg49' by m. ghosh and g. nanda, febs letters 330 (1993) 275-278 by d. ananthapadmanavan (institute of microbial technology, chandigarh, india). 19968925916
biotechnological production of oil: fatty acid composition of microbial oil.the fatty acid profile and the fatty acid composition of microbial lipids obtained from molds revealed that oil from aspergillus sydowii, fusarium oxysporum and f. equisetti had a high percentage of unsaturated fatty acids, particularly oleic acid, and had a similarity to the edible oils, groundnut and palm oil. this study sheds light on the possibilities of exploring the use of these oils as supplement to other edible fats and for other non-edible industrial purposes.199910540990
the first case of obstructing bronchial aspergillosis caused by aspergillus sydowi. 200414732333
cellular responses in sea fan corals: granular amoebocytes react to pathogen and climate stressors.climate warming is causing environmental change making both marine and terrestrial organisms, and even humans, more susceptible to emerging diseases. coral reefs are among the most impacted ecosystems by climate stress, and immunity of corals, the most ancient of metazoans, is poorly known. although coral mortality due to infectious diseases and temperature-related stress is on the rise, the immune effector mechanisms that contribute to the resistance of corals to such events remain elusive. in ...200818364996
within-host disease ecology in the sea fan gorgonia ventalina: modeling the spatial immunodynamics of a coral-pathogen interaction.we develop a spatially explicit model for the within-host interactions between a fungal pathogen and the immune response by its coral host. the model is parameterized for the recent epizootic of aspergillus sydowii in the sea fan gorgonia ventalina, but its structure is adaptable to many other diseases attacking corals worldwide, fungal infections in other invertebrates and plants, and opportunistic fungal infections in vertebrates. model processes include pathogen growth and spread through cons ...200718171161
host habitat assessment by a parasitoid using fungal volatiles.the preference-performance hypothesis predicts that oviposition preference of insects should correlate with host suitability for offspring development. therefore, insect females have to be able to assess not only the quality of a given host but also the environmental conditions of the respective host habitat. chemical cues are a major source of information used by insects for this purpose. primary infestation of stored grain by stored product pests often favors the intense growth of mold. this c ...200717284315
a simple polymerase chain reaction-sequencing analysis capable of identifying multiple medically relevant filamentous fungal species.due to the accumulating evidence that suggests that numerous unhealthy conditions in the indoor environment are the result of abnormal growth of the filamentous fungi (mold) in and on building surfaces it is necessary to accurately determine the organisms responsible for these maladies and to identify them in an accurate and timely manner. historically, identification of filamentous fungal (mold) species has been based on morphological characteristics, both macroscopic and microscopic. these met ...200617039272
peritonitis caused by aspergillus sydowii in a patient undergoing continuous ambulatory peritoneal dialysis.we describe a patient who received continuous ambulatory peritoneal dialysis and developed catheter-related peritonitis caused by aspergillus sydowii. identification of a. sydowii was confirmed by the sequence analysis of the ribosomal rna genes. the patient remained well after removal of the peritoneal catheter without administration of any anti-fungal agent.200516230197
presence of aspergillus sydowii, a pathogen of gorgonian sea fans in the marine sponge spongia obscura.the fungus aspergillus sydowii is the causative agent of epidemics that affect gorgonian corals (sea fans) and has significantly affected their populations in the caribbean sea. we have isolated a strain of a. sydowii from healthy marine sponges (spongia obscura) collected in bahamian inshore waters. after its identification on the basis of morphology, molecular markers and chemical profiling followed by pathogenicity tests, we found this strain to be highly similar to a strain isolated from dis ...200919279670
filamentous fungi associated with mosquito larvae (diptera: culicidae) in municipalities of the brazilian amazon.several species of the family culicidae are important vectors of diseases in humans and other animals. immature stages are filter-feeders of organic particulate matter and microorganisms. studies on microbial diversity can contribute to the discovery of new substances that can be used in the pharmaceutical industry for food or for biological control. the aim of this study was to isolate and identify the fungi associated with culicidae larvae found in different habitats (natural and artificial), ...200919618051
assessment of ribosomal large-subunit d1-d2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important aspergillus species.molecular approaches are now being developed to provide a more rapid and objective identification of fungi compared to traditional phenotypic methods. ribosomal targets, especially the large-subunit rna gene (d1-d2 region) and internal transcribed spacers 1 and 2 (its1 and its2 regions), have shown particular promise for the molecular identification of some fungi. we therefore conducted an assessment of these regions for the identification of 13 medically important aspergillus species: aspergill ...200515872227
arachnomyces kanei (anamorph onychocola kanei) sp. nov., from human nails.five isolates of a slow-growing cycloheximide resistant hyphomycetous fungus were obtained from nail specimens and investigated for their relationship to onychocola canadensis (teleomorph arachnomyces nodosetosus), a known agent of onychomycosis. in one patient diagnosed with superficial white onychomycosis, etiology was confirmed by a nail sample showing atypical filaments in direct microscopy, and by a follow-up specimen yielding cultures of the same fungus. a case of mixed infection with aspe ...200212521121
development of molecular tools for the mulundocandin producer aspergillus sydowii: dna-mediated transformation and reporter gene expression.the echinocandin-type antimycotic mulundocandin and its derivatives are produced by the filamentous fungus aspergillus sydowii (strain fh2551). these agents have been considered as a potential drug to treat immunocompromised patients who suffer from severe opportunistic fungal infections. in order to generate strains with a modified mulundocandin biosynthesis, we developed molecular tools for genetic engineering of a. sydowii as an alternative to conventional strain improvement procedures. for o ...200211956745
purification and some properties of a xylanase from aspergillus sydowii mg49.volume 62, no. 10, p. 3915, author's correction (ghosh and nanda). errors were made in the original identification of the microorganism used in this study and in the reporting of the corrected species identification. the culture resubmitted to the american type culture collection was identified by dr. frank dugan as aspergillus flavus, not aspergillus citrinum, and is available as atcc 96916. [this corrects the article on p. 3915b in vol. 62.].199716535533
isolation of brazilian marine fungi capable of growing on ddd pesticide.the fungi aspergillus sydowii ce15, aspergillus sydowii ce19, aspergillus sydowii gc12, bionectria sp. ce5, penicillium miczynskii gc5, penicillium raistrickii ce16 and trichoderma sp. gc1, isolated from marine sponges geodia corticostylifera and chelonaplysylla erecta, were evaluated for their ability to grow in the presence of ddd pesticide. increasing concentrations of ddd pesticide, i.e., 5.0 mg (1.56 × 10⁻¹² mmol), 10.0 mg (3.12 × 10⁻²) mmol) and 15.0 mg (4.68 × 10⁻² mmol) in solid and liqu ...201120533078
marine fungi aspergillus sydowii and trichoderma sp. catalyze the hydrolysis of benzyl glycidyl ether.whole cells of the marine fungi aspergillus sydowii gc12, penicillium raistrickii ce16, p. miczynskii gc5, and trichoderma sp. gc1, isolated from marine sponges of the south atlantic ocean (brazil), have been screened for the enzymatic resolution of (±)-2-(benzyloxymethyl)oxirane (benzyl glycidyl ether; 1). whole cells of a. sydowii gc12 catalyzed the enzymatic hydrolysis of (r,s)-1 to yield (r)-1 with an enantiomeric excess (ee) of 24-46% and 3-(benzyloxy)propane-1,2-diol (2) with ee values <10 ...201120549284
hypersaline waters - a potential source of foodborne toxigenic aspergilli and penicillia.previous studies of hypersaline environments have revealed the dominant presence of melanized yeast-like fungi and related cladosporium spp. in this study, we focused on the genera aspergillus and penicillium and their teleomorphic forms. from oligotrophic and eutrophic hypersaline waters around the world, 60 different species were identified, according to their morphological characteristics and extrolite profiles. for the confirmation of five new species, additionally, sequence analysis of the ...201121477006
isolation and characterization of microsatellite loci in aspergillus sydowii, a pathogen of caribbean sea fan corals.here we report on nine microsatellite loci designed for aspergillus sydowii, a widely distributed soil saprobe that is also the pathogenic agent of aspergillosis in caribbean sea fan corals. primers were tested on 20 a. sydowii isolates from the caribbean, 17 from diseased sea fans and three from environmental sources. all loci were polymorphic and exhibited varying degrees of allelic diversity (three to nine alleles). gene diversity (expected heterozygosity) ranged from 0.353 to 0.821. these pr ...200821585764
sesquiterpene and xanthone derivatives from the sea fan-derived fungus aspergillus sydowii psu-f154.three new sesquiterpenes, named aspergillusenes a and b and (+)-(7s)-7-o-methylsydonic acid, and two new hydrogenated xanthone derivatives, named aspergillusones a and b, were isolated from the sea fan-derived fungus aspergillus sydowii psu-f154 together with 10 known compounds. their structures were identified on the basis of spectroscopic data. the isolated compounds were evaluated for their antioxidant activity.201121718031
purification and some properties of a xylanase from aspergillus sydowii mg49.volume 60, no. 12, p 4620-4623: errors appear to have been made in the original identification of the microorganism used in this study and in the cultures submitted in 1993 to both the american type culture collection (atcc) and the indian institute of microbial technology. a resubmitted culture has now been assigned to the species aspergillus citrinum by dr. frank dugan of the atcc. the authors have confirmed that the resubmitted culture produces xylanase, so that the major findings of the orig ...199616535435
[comparative estimation of biocides and crown-ethers effect on micromycete-destructors cultures].influence of catamine ab, catapine, macrocyclic polyethers (crown-ethers [15]c-5, b[15]c-5, [18]c-6, b[18]c-6, dch[18]c-6, da[18]c-6) and mixtures of catapine with crown-ethers on fungal test-cultures of aspergillus sydowii (bain. et sart.) thom et church, cladosporium cladosporioides (fr.) de vries and penicillium tardum thom. it is shown, that solutions of catamine ab show the inhibiting effect in concentrations which are one level lower than in catapine. inhibition influence on the growth of ...200212190025
composition and antimicrobial activity of the essential oil of heracleum thomsonii (clarke) from the cold desert of the western himalayas.volatile oil composition of hydro-distilled (hd) and supercritical carbon dioxide (sc-co(2)) essential oil of freshly collected aerial parts of heracleum thomsonii (umbeliferae) from the western himalayas was studied by gc-fid and gc-ms. results revealed qualitative and quantitative dissimilarity in the composition of hydro-distilled and sc-co(2) extracted oils. nineteen constituents, which accounted for 89.32% of total constituents in hd oil, represented by limonene (4.31%), (z)-β-ocimene (3.69 ...201121854172
gene cloning and functional characterization by heterologous expression of the fructosyltransferase of aspergillus sydowi iam 2544.we have purified a fructosyltransferase from conidia of the inulin-producing fungus aspergillus sydowi iam 2544 and obtained peptide sequences from proteolytic fragments of the protein. with degenerated primers, we amplified a pcr fragment that was used to screen a cdna library. the fructosyltransferase gene from aspergillus sydowi (embl accession no. aj289046) is expressed in conidia, while no expression could be detected in mycelia by northern blot analysis of mycelial rna. the gene encodes a ...200111133467
commentary on: 'thermostability of beta-xylosidase from aspergillus sydowii mg49' by m. ghosh and g. nanda, febs letters 330, 275-278 (1993) 19968647261
metagenomic analysis of medicinal cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests.background: the presence of bacteria and fungi in medicinal or recreational cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. this study evaluated two widely used culture-based platforms for total yeast and mold (tym) testing marketed by 3m corporation and biomérieux, in comparison with a quantitative pcr (qpcr) approach marketed by medicinal genomics corporation. methods: a set of 15 medicinal cannabis samples were analyzed using 3m and bi ...201627853518
season and tissue type affect fungal endophyte communities of the indian medicinal plant tinospora cordifolia more strongly than geographic location.a total of 1,151 endophytic fungal isolates representing 29 taxa were isolated from symptom-less, surface-sterilized segments of stem, leaf, petiole, and root of tinospora cordifolia which had been collected at three locations differing in air pollution in india (ramnagar, banaras hindu university, maruadih) during three seasons (summer, monsoon, winter). endophytes were most abundant in leaf tissues (29.38% of all isolates), followed by stem (18.16%), petiole (10.11%), and root segments (6.27%) ...201222430503
isolates identification and characteristics of microorganisms in biotrickling filter and biofilter system treating h2s and nh3.a combination system of biotrickling filter (btf) and biofilter (bf), adopting surfactant-modified clinoptilolite and surfactant-modified wood chip as the media respectively, was applied to treat h2s and nh3 simultaneously. the identification and sole carbon sources utilization patterns of isolates in the combination system were studied by biolog system. the isolates were identified as bacillus sphaericus, geobacillus themoglucosidasius (55 degrees c) and micrococcus luteus (atcc 9341) in btf, a ...200717966875
the opportunistic coral pathogen aspergillus sydowii contains dddp and makes dimethyl sulfide from dimethylsulfoniopropionate.the ascomycete aspergillus sydowii is associated with a serious epizootic of sea fan corals in the caribbean. corals are rich in the compatible solute, dimethylsulfoniopropionate (dmsp), produced by their symbionts, the dinoflagellate symbiodinium. as other aspergillus species can catabolize dmsp, liberating dimethyl sulfide (dms) in the process, we tested a. sydowii strains, obtained from diseased corals and other environments, for this ddd(+) phenotype. all the strains, irrespective of their g ...201019776768
[aspergillus species in hospital environments with pediatric patients in critical condition].aspergillus is a group of opportunistic fungi that cause infections, with high morbimortality in immunosuppressed patients. aspergillus fumigatus is the most frequent species in these infections, although the incidence of other species has increased in the last few years.201424120420
bio-protective potential of lactic acid bacteria isolated from fermented wax gourd.the antifungal activities of 85 lactic acid bacteria strains isolated from fermented wax gourd against the four fungal species, penicillium oxalicum, aspergillus flavus, aspergillus sydowii, and mucor racemosus, were determined. inhibitory activity against at least one or more fungal species was observed with 27 weissella cibaria and 11 weissella paramesenteroides strains. among these strains, w. cibaria 861006 and w. paramesenteroides 860509 showed greater inhibitory activities and were therefo ...201222307833
thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production.nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at brazilian cerrado and identified up to species level on the basis of the gene sequencing of 5.8s-its and 26s ribosomal dna regions. four species were identified: kluyveromyces marxianus, aspergillus niger, aspergillus sydowii and aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. among them, th ...201726413077
vea of aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture.aspergillus species are highly abundant fungi worldwide. their conidia are among the most dominant fungal spores in the air. conidia are formed in chains on the vesicle of the asexual reproductive structure called the conidiophore. here, it is shown that the velvet protein vea of aspergillus niger maximizes the diameter of the vesicle and the spore chain length. the length and width of the conidiophore stalk and vesicle were reduced nearly twofold in a δvea strain. the latter implies a fourfold ...201525367340
isotopic labeling and antifungal resistance as tracers of gut passage of the sea fan pathogen aspergillus sydowii.vectors play a critical role in the ecology of infectious disease by facilitating between host transmission, emphasizing the multi-species nature of disease. corals are suffering an onslaught of infectious diseases, yet we know little about the role of vector species in the ecology of these epizootics. the infection of octocorals by the fungus aspergillus sydowii is a widespread caribbean coral disease. the snail cyphoma gibbosum is a likely vector species because it is a specialist predator of ...200919899343
microbial diseases of corals and global warming.coral bleaching and other diseases of corals have increased dramatically during the last few decades. as outbreaks of these diseases are highly correlated with increased sea-water temperature, one of the consequences of global warming will probably be mass destruction of coral reefs. the causative agent(s) of a few of these diseases have been reported: bleaching of oculina patagonica by vibrio shiloi; black band disease by a microbial consortium; sea-fan disease (aspergillosis) by aspergillus sy ...200212071977
thermostability of beta-xylosidase from aspergillus sydowii mg49.heating of aspergillus beta-xylosidase at 85 degrees c +/- 1 degree c and ph 5.5-6.0 (optimum for activity), causes irreversible, covalent thermoinactivation of the enzyme, involving oxidation of the thiol groups that are required for catalysis. exogenous addition of cysteine, dtt, gsh and mercaptoethanol stabilizes the enzyme by extending its half-life. a similar effect is also exhibited by bivalent cations like mg2+, mn2+, co2+, ca2+ and zn2+ while, on the other hand cu2+ accelerates thermoina ...19938375499
exploration of cultivable fungal communities in deep coal-bearing sediments from ∼1.3 to 2.5 km below the ocean floor.although subseafloor sediments are known to harbour a vast number of microbial cells, the distribution, diversity, and origins of fungal populations remain largely unexplored. in this study, we cultivated fungi from 34 of 47 deep coal-associated sediment samples collected at depths ranging from 1289 to 2457 m below the seafloor (mbsf) off the shimokita peninsula, japan (1118 m water depth). we obtained a total of 69 fungal isolates under strict contamination controls, representing 61 ascomycota ...201728028923
onychomycosis, tinea pedis and tinea manuum caused by non-dermatophytic filamentous fungi.over 4000 cases of fungal nail, sole and palm infection recorded during a three-year study period were investigated to determine the prevalence and significance of etiologic agents other than dermatophytes and yeasts. littman's oxgall agar, which permitted isolation of cycloheximide-sensitive species but restricted overgrowth by fast growing contaminants, was used to promote isolation of these disease agents. non-dermatophytic filamentous fungi made up 2.3% of the confirmed etiologic agents obta ...19892533665
a case of onychomycosis due to aspergillus sydowii diagnosed using dna sequence analysis.we report a case of disto-lateral subungal onychomycosis of the first toe of the right foot of a 33-year-old female. the causative agent was identified as aspergillus sydowii based on dna sequencing and the macroscopic and microscopic characteristics of the culture. initially, the medial one-third of the right first toenail was streaked with numerous yellow longitudinal stripes that had fused, with no symptoms of tinea pedis. treatment with oral terbinafine and itraconazole was ineffective. base ...200818254756
globally panmictic population structure in the opportunistic fungal pathogen aspergillus sydowii.recent outbreaks of new diseases in many ecosystems are caused by novel pathogens, impaired host immunity, or changing environmental conditions. identifying the source of emergent pathogens is critical for mitigating the impacts of diseases, and understanding the cause of their recent appearances. one ecosystem suffering outbreaks of disease in the past decades is coral reefs, where pathogens such as the fungus aspergillus sydowii have caused catastrophic population declines in their hosts. aspe ...200818684135
biodegradation of the pyrethroid pesticide esfenvalerate by marine-derived fungi.esfenvalerate biodegradation by marine-derived fungi is reported here. esfenvalerate (s,s-fenvalerate) and its main metabolites [3-phenoxybenzaldehyde (pbald), 3-phenoxybenzoic acid (pbac), 3-phenoxybenzyl alcohol (pbalc), and 2-(4-chlorophenyl)-3-methylbutyric acid (clac)] were quantitatively analyzed by a validated method in triplicate experiments. all the strains (penicillium raistrickii cbmai 931, aspergillus sydowii cbmai 935, cladosporium sp. cbmai 1237, microsphaeropsis sp. cbmai 1675, ac ...201627381569
biodegradation of the organophosphate trichlorfon and its major degradation products by a novel aspergillus sydowii pa f-2.trichlorfon (tcf) is an important organophosphate pesticide in agriculture. however, limited information is known about the biodegradation behaviors and kinetics of this pesticide. in this study, a newly isolated fungus (pa f-2) from pesticide-polluted soils was identified as aspergillus sydowii on the basis of the sequencing of internal transcribed spacer rdna. this fungus degraded tcf as sole carbon, sole phosphorus, and sole carbon-phosphorus sources in a mineral salt medium (msm). optimal tc ...201627161040
molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia.endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems.201627099679
aspergillus sydowii marine fungal bloom in australian coastal waters, its metabolites and potential impact on symbiodinium dinoflagellates.dust has been widely recognised as an important source of nutrients in the marine environment and as a vector for transporting pathogenic microorganisms. disturbingly, in the wake of a dust storm event along the eastern australian coast line in 2009, the continuous plankton recorder collected masses of fungal spores and mycelia (~150,000 spores/m³) forming a floating raft that covered a coastal area equivalent to 25 times the surface of england. cultured a. sydowii strains exhibited varying meta ...201626999164
stable symbiodinium composition in the sea fan gorgonia ventalina during temperature and disease stress.like most caribbean octocorals, gorgonia ventalina, the common sea fan, harbors endosymbiotic dinoflagellates belonging to the genus symbiodinium. when stressed, the host can lose these algal symbionts, a phenomenon termed "bleaching." many cnidarians host multiple types of algal symbionts within the genus symbiodinium, and certain types of algae may be more tolerant of stress than others. we examined the effects of temperature, temperature-induced bleaching, and infection by aspergillus sydowii ...200516382170
effect of inoculation techniques and relative humidity on the growth of molds on the surfaces of yellow layer cakes.four inoculation techniques were compared for initiation of growth on cake surfaces: spot, air cabinet, spray (atomizer), and talc addition methods. molds were isolated from commercial cakes and were identified as aspergillus sydowii, aspergillus ochraceus, penicillium funiculosum, and eurotium herbariorum. cake surfaces were inoculated with mold spores and incubated under three equilibrium relative humidity (erh) levels: 97, 85, and 75%. random contamination by spores in a ventilated air cabine ...199816349479
discrimination of three genetically close aspergillus species by using high resolution melting analysis applied to indoor air as case study.indoor air pollution caused by fungal contamination is suspected to have a public health impact. monitoring of the composition of the indoor airborne fungal contaminants is therefore important. to avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. among these molecular methods, the polymerase chain reaction (pcr) and the real-time pcr are the most frequently used tools for indoor fungal detection. however, even if these tool ...201728376723
diversity and bioprospection of fungal community present in oligotrophic soil of continental antarctica.we surveyed the diversity and capability of producing bioactive compounds from a cultivable fungal community isolated from oligotrophic soil of continental antarctica. a total of 115 fungal isolates were obtained and identified in 11 taxa of aspergillus, debaryomyces, cladosporium, pseudogymnoascus, penicillium and hypocreales. the fungal community showed low diversity and richness, and high dominance indices. the extracts of aspergillus sydowii, penicillium allii-sativi, penicillium brevicompac ...201525809294
biohydroxylation of (-)-ambrox®, (-)-sclareol, and (+)-sclareolide by whole cells of brazilian marine-derived fungi.a screening was performed using nine marine-derived fungi as biocatalysts and the natural products (-)-ambrox® (1), (-)-sclareol (2), and (+)-sclareolide (3) in order to select the microorganisms able to catalyze the biooxidation of these compounds. it was observed that only the aspergillus sydowii cbmai 934, botryosphaeria sp., eutypella sp., and xylaria sp. presented active oxidoreductases and catalyzed the regioselective hydroxylation in the natural products. the hydroxylated metabolites obta ...201525634054
aspergillosis in the common sea fan gorgonia ventalina: isolation of waterborne hyphae and spores.the octocoral disease aspergillosis is caused by the terrestrial fungus aspergillus sydowii. the possibility of secondary (horizontal) transmission of aspergillosis among common sea fans gorgonia ventalina would require waterborne transmission of hyphae and/or spores. a laboratory filtration experiment confirmed that fungal hyphae and spores were shed into the water by infected fans. this suggests that secondary infection might be possible in this species. it remains to be determined whether hea ...201424991852
new metabolites from the sponge-derived fungus aspergillus sydowii j05b-7f-4.two new metabolites, diorcinolic acid (1) and β-d-glucopyranosyl aspergillusene a (8), together with six diphenylethers (2-7), a diketopiperazine (9), a chromone (10) and a xanthone (11) were isolated from the fungus aspergillus sydowii derived from the marine sponge stelletta sp. the planar structures and their relative configurations were elucidated by analysing 1d, 2d nmr and hresims data. compound 8 is the first glycoside of phenolic bisabolane sesquiterpenes. compounds 1 and 8 exhibited mil ...201728278674
influence of fowl uropygial gland and its secretory lipid components on the growth of skin surface fungi of fowl.fungal species, which were shown to colonize consistently on the skin surface of the breast region of adult (1 year old) white leghorn fowl, were identified as aspergillus sydowii, a. tamarii, a. rugulosus and absidia corymbifera. of these, a. sydowii and a. tamarii were the dominant forms. two species of fungi, namely, aspergillus niger and scopulariopsis brevicaulis were shown to be present in the cultures of the scrubbings from breast skin surface after 60 days of captivity of the fowls. exti ...199910865889
mycobiota and aflatoxins in raw materials and pet food in brazil.commercial feedstuffs are a basic element in modern pet husbandry in the world. in dogs, the effect of mycotoxins is severe and can lead to death. few reports on the influence of dietary mycotoxins were found in the scientific literature. the aims of this work were to isolate and identify the mycoflora and to determine the aflatoxins (afs) natural occurrence in raw materials and ready dry pet food. therefore, the aflatoxigenic capacity of aspergillus flavus species was investigated. aspergillus ...200818477320
clinical effects of gamma-radiation-resistant aspergillus sydowii on germ-free mice immunologically prone to inflammatory bowel disease.we report and investigated a case of inadvertent contamination of 125 mice (housed in two germ-free positive-pressurized isolators) with emerging human and coral pathogen aspergillus sydowii. the infected mice correspond to genetic line samp1/yitfc, which have 100% immune predisposition to develop crohn's disease-like spontaneous pathologies, namely, inflammatory bowel disease (ibd). pathogen update based on a scoping review of the literature and our clinical observations and experimentation are ...201627630775
purification and biochemical characterization of two xylanases from aspergillus sydowii sbs 45.two xylanases were isolated and purified from crude culture filtrate of aspergillus sydowii sbs 45 after 9 days of growth on wheat bran containing 0.5% (w/v) birch wood xylan as the carbon source under solid-state fermentation. after a three-step purification scheme involving ammonium sulfate precipitation, gel filtration chromatography (sephadex g-200), and anion exchange chromatography (deae-sephadex a-50), xylanase i was purified 93.41 times, and xylanase ii was purified 77.40 times with yiel ...200818500584
peroxidase activity and inducibility in the sea fan coral exposed to a fungal pathogen.the enzymatic defense mechanisms of gorgonia ventalina to the fungal pathogen aspergillus sydowii may play important roles in colony resistance to infection. in this study, we examined the role of the superfamily of peroxidase enzymes in the coral response to a naturally occurring pathogen. we examined the inducibility of peroxidases by experimentally exposing corals to a. sydowii and found that peroxidase activity was induced after an 8 day incubation period. in contrast, naturally infected cor ...200717064941
antifouling indole alkaloids from two marine derived fungi.in order to find non-toxic antifouling natural products from marine microorganisms, the chemical constituents of two marine derived fungi penicillium sp. and aspergillus sydowii have been investigated under bio-guided fractionation. a new indolyl diketopiperazine compound, penilloid a (1), together with 15 known ones were isolated from these two strains. the structure of 1 was elucidated on the basis of nmr and mass spectra. some alkaloids showed significant antifouling and antibacterial activit ...201323678803
aspergillus mulundensis sp. nov., a new species for the fungus producing the antifungal echinocandin lipopeptides, mulundocandins.the invalidly published name aspergillus sydowii var. mulundensis was proposed for a strain of aspergillus that produced new echinocandin metabolites designated as the mulundocadins. reinvestigation of this strain (y-30462=dsmz 5745) using phylogenetic, morphological, and metabolic data indicated that it is a distinct and novel species of aspergillus sect. nidulantes. the taxonomic novelty, aspergillus mulundensis, is introduced for this historically important echinocandin-producing strain. the ...201626464011
stereoselective bioreduction of 1-(4-methoxyphenyl)ethanone by whole cells of marine-derived fungi.nine strains of marine-derived fungi (aspergillus sydowii ce15, a. sydowii ce19, aspergillus sclerotiorum cbmai 849, bionectria sp. ce5, beauveria felina cbmai 738, cladosporium cladosporioides cbmai 857, mucor racemosus cbmai 847, penicillium citrinum cbmai 1186, and penicillium miczynskii gc5) were screened, catalyzing the asymmetric bioreduction of 1-(4-methoxyphenyl)ethanone 1 to its corresponding 1-(4-methoxyphenyl)ethanol 2. a. sydowii ce15, a. sclerotiorum cbmai 849, and bionectria sp. ce ...201121842151
tumor induction by a single subcutaneous injection of sterigmatocystin in newborn mice.sterigmatocystin, a mycotoxin produced by aspergillus versicolor, aspergillus sydowi, aspergillus nidulans, and a species of bipolaris, was given to newborn balb/c x dba/2f1 (hereafter referred to as cd2f1) mice by a single s.c. administration in 1% gelatin suspension. in an acute toxicity study, the maximum tolerated dose of sterigmatocystin was 5 mug/g body weight. in a chronic study, a single s.c. injection of 5, 1, or 0.5 mug/g body weight gave rise to high incidences of lung and liver adeno ...19761268822
[a case of onychomycosis caused by aspergillus sydowii].onychomyosis caused by aspergillus sydowii is rare and difficult to diagnose. we report a case in which this disease was diagnosed by molecular-biological methods. the patient was a 53-year-old woman without any notable past history. she visited our hospital complaining of itching around the right first toenail in june 2010, although she had noticed nail opacification for 4 years. opaque thickening of the nail, covering 57.3% of the normal nail area, was observed. direct microscopic examination ...201223149356
production and chemical characterization of pigments in filamentous fungi.production of pigments by filamentous fungi is gaining interest owing to their use as food colourants, in cosmetics and textiles, and because of the important biological activities of these compounds. in this context, the objectives of this study were to select pigment-producing fungi, identify these fungi based on internal transcribed spacer sequences, evaluate the growth and pigment production of the selected strains on four different media, and characterize the major coloured metabolites in t ...201626341482
new diketopiperazine dimer from a filamentous fungal isolate of aspergillus sydowii. 201526040447
stereoselective bioreduction of 1-(4-methoxyphenyl)ethanone by whole cells of marine-derived fungi.nine strains of marine-derived fungi (aspergillus sydowii ce15, a. sydowii ce19, aspergillus sclerotiorum cbmai 849, bionectria sp. ce5, beauveria felina cbmai 738, cladosporium cladosporioides cbmai 857, mucor racemosus cbmai 847, penicillium citrinum cbmai 1186, and penicillium miczynskii gc5) were screened, catalyzing the asymmetric bioreduction of 1-(4-methoxyphenyl)ethanone 1 to its corresponding 1-(4-methoxyphenyl)ethanol 2. a. sydowii ce15 and bionectria sp. ce5 produced the enantiopure ( ...201122160343
a cardinal model to describe the effect of water activity on the growth of moulds.a simple model was proposed to describe the effect of water activity (aw) on the radial growth rate of moulds. this model is deduced from the cardinal model family proposed by rosso in 1995, which is only defined from cardinal values of environmental factors (minimum, optimum and maximum values), the growth rate observed at the optimal value of the environmental factors, and n, a shape parameter. for aw, a simple form of cardinal model is proposed. this form is obtained for n = 2 and aw(max) = 1 ...200111246910
aspergillus sydowii and other potential fungal pathogens in gorgonian octocorals of the ecuadorian pacific.emerging fungal diseases are threatening ecosystems and have increased in recent decades. in corals, the prevalence and consequences of these infections have also increased in frequency and severity. coral reefs are affected by an emerging fungal disease named aspergillosis, caused by aspergillus sydowii. this disease and its pathogen have been reported along the caribbean and pacific coasts of colombia. despite this, an important number of coral reefs worldwide have not been investigated for th ...201627902710
species diversity of aspergillus section versicolores in clinical samples and antifungal susceptibility.aspergillus section versicolores includes species of clinical relevance and many others that have been poorly studied but are occasionally found in clinical samples. the aim of this study was to investigate, using a multilocus phylogenetic approach, the spectrum of species of the section versicolores and to determine their in vitro antifungal susceptibility. the study was based on a set of 77 clinical isolates from different usa medical centres, which had been previously identified as belonging ...201627742099
australian dust storm associated with extensive aspergillus sydowii fungal "bloom" in coastal waters.a massive central australian dust storm in september 2009 was associated with abundant fungal spores (150,000/m(3)) and hyphae in coastal waters between brisbane (27°s) and sydney (34°s). these spores were successfully germinated from formalin-preserved samples, and using molecular sequencing of three different genes (the large subunit rrna gene [lsu], internal transcribed spacer [its[, and beta-tubulin gene), they were conclusively identified as aspergillus sydowii, an organism circumstantially ...201424657868
biotransformation of methylphenylacetonitriles by brazilian marine fungal strain aspergillus sydowii cbmai 934: eco-friendly reactions.this study reports the biotransformation of methylphenylacetonitriles by brazilian marine filamentous fungus aspergillus sydowii cbmai 934 under eco-friendly reaction conditions. the phenylacetonitrile 1, 2-methylphenylacetonitrile 2, 3-methylphenylacetonitrile 3, and 4-methylphenylacetonitrile 4 were quantitatively biotransformed into 2-hydroxyphenylacetic 1a, 2-methylphenylacetic acid 2a, 3-methylphenylacetic acid 3a, and 4-methylphenylacetic acid 4a by enzymatic processes using whole cell as ...201424057165
[ecological and physiological variations of saprotrophic and clinical isolates of the microscopic fungus aspergillus sydowii (bainier & sartory) thom & church]. 201323718052
cytotoxic alkaloids and antibiotic nordammarane triterpenoids from the marine-derived fungus aspergillus sydowi.three new diketopiperazine alkaloids, 6-methoxyspirotryprostatin b (1), 18-oxotryprostatin a (2), and 14-hydroxyterezine d (3), with an oxaspiro[4.4]lactam moiety, 14-norpseurotin a (4), and the 29-nordammarane triterpenoid 6beta,16beta-diacetoxy-25-hydroxy-3,7-dioxy-29-nordammara-1,17(20)-dien-21-oic acid (5), as well as 12 known compounds (6- 17), were isolated from the ethyl acetate extract of a marine-derived fungal strain, aspergillus sydowi pfw1-13. the structures of compounds 1- 5 were el ...200818505285
an epigenetic modifier enhances the production of anti-diabetic and anti-inflammatory sesquiterpenoids from aspergillus sydowii.the addition of a dna methyltransferase inhibitor, 5-azacytidine, to aspergillus sydowii fungus culture broth changed its secondary metabolites profile. analysis of the culture broth extract led to the isolation of three new bisabolane-type sesquiterpenoids: (7s)-(+)-7-o-methylsydonol (1), (7s,11s)-(+)-12-hydroxysydonic acid (2) and 7-deoxy-7,14-didehydrosydonol (3), along with eight known compounds. the isolated compounds were evaluated for their anti-diabetic and anti-inflammatory activities. ...201323647825
aspergillus section versicolores: nine new species and multilocus dna sequence based phylogeny.β-tubulin, calmodulin, internal transcribed spacer and partial lsu-rdna, rna polymerase 2, dna replication licensing factor mcm7, and pre-rrna processing protein tsr1 were amplified and sequenced from numerous isolates belonging to aspergillus sect. versicolor. the isolates were analyzed phylogenetically using the concordance model to establish species boundaries. aspergillus austroafricanus, a. creber, a. cvjetkovicii, a. fructus, a. jensenii, a. puulaauensis, a. subversicolor, a. tennesseensis ...201223155501
gamma irradiation of cultural artifacts for disinfection using monte carlo simulations.in this study, it has been investigated the disinfection of korean cultural artifacts by gamma irradiation, simulating the absorbed dose distribution on the object with the monte carlo methodology. fungal contamination was identified on two traditional korean agricultural tools, hongdukkae and holtae, which had been stored in a museum. nine primary species were identified from these items: bjerkandera adusta, dothideomycetes sp., penicillium sp., cladosporium tenuissimum, aspergillus versicolor, ...201222940410
biotransformation of phenylacetonitrile to 2-hydroxyphenylacetic acid by marine fungi.marine fungi belonging to the genera aspergillus, penicillium, cladosporium, and bionectria catalyzed the biotransformation of phenylacetonitrile to 2-hydroxyphenylacetic acid. eight marine fungi, selected and cultured with phenylacetonitrile in liquid mineral medium, catalyzed it quantitative biotransformation to 2-hydroxyphenylacetic acid. in this study, the nitrile group was firstly hydrolysed, and then, the aromatic ring was hydroxylated, producing 2-hydroxyphenylacetic acid with 51 % yield ...201322790719
bioconversion of iodoacetophenones by marine fungi.nine marine fungi (aspergillus sclerotiorum cbmai 849, aspergillus sydowii ce19, beauveria felina cbmai 738, mucor racemosus cbmai 847, penicillium citrinum cbmai 1186, penicillium miczynskii ce16, p. miczynskii gc5, penicillium oxalicum cbmai 1185, and trichoderma sp. gc1) catalyzed the asymmetric bioconversion of iodoacetophenones 1-3 to corresponding iodophenylethanols 6-8. all the marine fungi produced exclusively (s)-ortho-iodophenylethanol 6 and (s)-meta-iodophenylethanol 7 in accordance t ...201222653656
biodegradation of methyl parathion by whole cells of marine-derived fungi aspergillus sydowii and penicillium decaturense.seven marine fungi strains (aspergillus sydowii cbmai 934, a. sydowii cbmai 935, a. sydowii cbmai 1241, penicillium decaturense cbmai 1234, penicillium raistrickii cbmai 931, p. raistrickii cbmai 1235, and trichoderma sp. cbmai 932) were screened by their growth in the presence of methyl parathion (mp) in a solid culture medium. the strains with best growth were a. sydowii cbmai 935 and p. decaturense cbmai 1234. biodegradation reactions were performed in 10, 20 and 30d in a malt extract liquid ...201424955826
enzymatic bleaching of kraft pulp by xylanase from aspergillus sydowii sbs 45.crude xylanase from aspergillus sydowii sbs 45 was tested for enzymatic bleaching of kraft (decker) pulp. after optimization of three parameters, consistency of pulp, retention time and enzyme dose, considerable increase in the release of uv and visible absorbance spectra of materials and reducing sugars was observed, which clearly indicated the action of xylanase on pulp. final brightness of pulp was increased from 29.42 to 70.42% and kappa number was reduced from 15.93 to 1.61, when 25 u of xy ...201023100849
multifaceted defense against antagonistic microbes in developing offspring of the parasitoid wasp ampulex compressa (hymenoptera, ampulicidae).effective antimicrobial strategies are essential adaptations of insects to protect themselves, their offspring, and their foods from microbial pathogens and decomposers. larvae of the emerald cockroach wasp, ampulex compressa, sanitize their cockroach hosts, periplaneta americana, with a cocktail of nine antimicrobials comprising mainly (r)-(-)-mellein and micromolide. the blend of these antimicrobials has broad-spectrum antimicrobial activity. here we explore the spatio-temporal pattern of depl ...201424886721
antimicrobial and antiviral sesquiterpenoids from sponge-associated fungus, aspergillus sydowii zsds1-f6. 201424736857
two new metabolites with cytotoxicities from deep-sea fungus, aspergillus sydowi yh11-2.two new compounds, 2, 3, 5-trimethyl-6-(3-oxobutan-2-yl)-4h-pyran-4-one (1) and (2r)-2, 3- dihydro-7-hydroxy-6, 8-dimethyl-2-[(e)-prop-1-enyl] chromen-4-one (2), together with six known compounds (3-8), were isolated from a deep-sea fungus, identified as aspergillus sydowi, by a bioassay-guided method. their structures were elucidated by spectroscopic methods and the cytotoxicities were evaluated by srb method.200717958319
[ sporulation features in saprophytic and clinical strains of the aspergillus sydowii (bain and sart.) thom and church under various environmental conditions ]. 201021774159
isolation and molecular characterization of polyvinyl chloride (pvc) plastic degrading fungal isolates.the recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. the present study was aimed to isolate and screen different soil fungi having potential to biodegrade pvc films. after 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on pvc films. on morphological as well as on 18rrna gene sequence and phylogenetic basis they were identified as phanerochaete chrysosporium pv1, lentinu ...201423686796
two new hetero-spirocyclic gamma-lactam derivatives from marine sediment-derived fungus aspergillus sydowi d2-6.two new hetero-spirocyclic gamma-lactams, azaspirofurans a (1) and b (2), had been isolated from a marine sediment-derived fungus aspergillus sydowi d2-6. their structures were determined by the chemical evidence and spectral analyses. compound 1 exhibited cytotoxicity against a549 cell line with the ic50 value of 10 microm by mtt method.201020422356
indole alkaloids from marine-derived fungus aspergillus sydowii scsio 00305. 201122186592
aspergillus species as emerging causative agents of onychomycosis.onychomycosis is a common nail infection caused by dermatophytes, non-dermatophyte molds (ndm), and yeasts. aspergillus species are emerging as increasing causes of toenail onychomycosis. the purpose of this study was species delineation of aspergillus spp. isolated from patients with onychomycosis.201525656408
aspergillus flavus contamination in two portuguese wastewater treatment plants.filamentous fungi from genus aspergillus were previously detected in wastewater treatment plants (wwtp) as being aspergillus flavus (a. flavus), an important toxigenic fungus producing aflatoxins. this study aimed to determine occupational exposure adverse effects due to fungal contamination produced by a. flavus complex in two portuguese wwtp using conventional and molecular methodologies. air samples from two wwtp were collected at 1 m height through impaction method. surface samples were coll ...201425072712
biotransformation of α-bromoacetophenones by the marine fungus aspergillus sydowii.the biotransformation reactions of α-bromoacetophenone (1), p-bromo-α-bromoacetophenone (2), and p-nitro-α-bromoacetophenone (3) by whole cells of the marine fungus aspergillus sydowii ce19 have been investigated. fungal cells that had been grown in artificial sea water medium containing a high concentration of chloride ions (1.20 m) catalysed the biotransformation of 1 to 2-bromo-1-phenylethanol 4 (56%), together with the α-chlorohydrin 7 (9%), 1-phenylethan-1,2-diol 9 (26%), acetophenone 10 (4 ...201019941024
isolation, characterization and transcriptome analysis of a novel antarctic aspergillus sydowii strain ms-19 as a potential lignocellulosic enzyme source.with the growing demand for fossil fuels and the severe energy crisis, lignocellulose is widely regarded as a promising cost-effective renewable resource for ethanol production, and the use of lignocellulose residues as raw material is remarkable. polar organisms have important value in scientific research and development for their novelty, uniqueness and diversity.201728558650
functional characterization of the n-terminal and c-terminal domains of a sesame group ii phytocystatin.phytocystatins are natural inhibitors of cysteine protease, and may regulate endo- or exo-genous proteolytic activities in plants. they are classified into group i and ii differing by the presence of c-terminal extension of group ii. a cdna fragment encoding a group ii phytosystatin, sicys was previously obtained from sesame seeds.201428510930
aspergillus sydowi infection of human finger nail.a case of aspergillus sydowi infection of left middle finger nail is described ' the presence of fungal hypae with phialids and spores on direct microscopy as well as in culture, the colour of the sub-ungual mass of the nail resembling the colour of the fungus in, culture' repeated isolations of a sydowi from the diseased tissue along with the absence of any established pathogenic species in the specimen are taken as evidences that this fungus was invading the nail tissue.201728211407
genetic diversity of aspergillus species isolated from onychomycosis and aspergillus hongkongensis sp. nov., with implications to antifungal susceptibility testing.thirteen aspergillus isolates recovered from nails of 13 patients (fingernails, n=2; toenails, n=11) with onychomycosis were characterized. twelve strains were identified by multilocus sequencing as aspergillus spp. (aspergillus sydowii [n=4], aspergillus welwitschiae [n=3], aspergillus terreus [n=2], aspergillus flavus [n=1], aspergillus tubingensis [n=1], and aspergillus unguis [n=1]). isolates of a. terreus, a. flavus, and a. unguis were also identifiable by matrix-assisted laser desorption/i ...201626658315
another possible risk for the mediterranean sea? aspergillus sydowii discovered in the port of genoa (ligurian sea, italy).aspergillus sydowii is a cosmopolitan fungus that has been responsible for the mass destruction of coral in the caribbean sea over the last 15years. to our knowledge, this study has found the first case of a. sydowii in the mediterranean sea, in marine-bottom sediments, water and calcareous shells of bivalve molluscs sampled during a campaign to characterise the mycobiota in the port of genoa (italy). the area is characterised by adverse environmental conditions (high turbidity, organic pollutio ...201728651864
antibacterial constituents from antarctic fungus, aspergillus sydowii sp-1.one new alkaloid, named as acremolin c (1), was isolated from static culture of antarctic fungus, aspergillus sydowii sp-1, in an investigation of the antimicrobial constituents of this antarctic microorganism, and its structure was determined by spectroscopic methods. additionally, four known compounds, cyclo-(l-trp-l-phe) (2), 4-hydroxyphenylacetic acid (3), (7s)-(+)-hydroxysydonic acid (4) and (7s, 11s)-(+)-12-hydroxysydonic acid (5), were isolated and identified. biological studies disclosed ...201728602098
identification of a consistent polyene component of purple pigment in diseased sclerites of caribbean corals across region, species, and insult agent.gorgonians respond to insult (damage and disease) by producing sclerites containing a purple pigment as opposed to the normal white sclerites. raman microscopy is used to study the purple areas of three species of diseased coral, gorgonia ventalina, pseudoplexaura porosa, and eunicea laciniata obtained from puerto rico. these spectra were compared to gorgonia ventalina samples previously reported that were obtained from san salvador, bahamas. spectra from two samples of g. ventalina that had bee ...201728591685
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