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[sequence analysis of bacterial transposon in nhx gene of populus euphratica].the united nations environment program estimates that approximately 20% of agricultural land and 50% of cropland in the world is salt-stressed. the gene nhx (na+/h+ exchanger) encodes functional protein that catalyzes the countertransport of na+ and h+ across membranes and may play an important role in plant salt tolerance. to clone the nhx from the wild plant populus euphratica collected in tarim basin and xinjiang wujiaqu district into a t-vector, designed primer was used to amplify 1kb nhx cd ...200315969097
[effect of translating water on vegetation at the lower reaches of tarim river].due to human irrational resource exploit in recent 50 years, many environment problems have appeared at the lower reaches of tarim river, the environment degradation of this region became seriously. in order to restore green corridor and improve the environment at the lower reaches of tarim river, the project of transporting water to the lower reaches of tarim river was carried out from june 14, 2000 and the water reached taitema lake finally. after two years' monitoring and analyzing in this re ...200314551951
[effect of insect pest on activated oxygen metabolism in lanceolate leaves of populus euphratica under different water condition].this paper studied the effect of insect pest on the activities of protective enzymes sod and cat and the contents of h2o2 and o2*- in lanceolate leaves of populus euphratica under different soil water conditions. the results showed that soil water deficiency did not increase sod and cat activities and h2o2 and o2*- contents in lanceolate leaves, but under this condition, insect pest increased them. when soil water was abundance, insect pest couldn't increase the activities of sod and cat and the ...200415320407
[niche characteristics of plants on four environmental gradients in middle reaches of tarim river].tarim river is the longest continental river in china. along its either bank, the natural vegetation belts are composed of arbors, shrubs and grasses. in this paper, the niche breadths and overlaps of nineteen species on four environmental gradients in the middle reaches of tarim river were calculated and analyzed. the results showed that the nineteen species could be divided into three groups, based on their average niche breadths. group one included populus euphratica, tamarix ramosissima, t. ...200415334943
antibacterial activity of turkish propolis and its qualitative and quantitative chemical composition.the antibacterial activity of propolis from different regions of turkey was studied, accompanied by tlc and gc-ms analyses of its chemical composition and spectrophotometric quantification of the most important active principles. all six samples were active against the bacterial test strains used; however, samples 1 (yozgat), 2 (izmir) and 3 (kayseri) were more active than samples 4 (adana), 5 (erzurum) and 6 (artvin). by tlc comparison all samples were found to contain poplar taxonomic markers ...200515830845
molecular characterization of penhad1: the first member of the nhad na+/h+ antiporter family of plant origin.penhad1 encodes a putative na+/h+ antiporter from the salt-resistant tree populus euphratica. it is the first characterization of a member of the nhad type ion transporter family of plant origin. homology searches revealed its close relation to functionally characterized microbial na+/h+ antiporters vpnhad and vcnhad. na+/h+ antiporters have proven to play a key role in salt resistance, both in plants and bacteria. under salt stress transcript levels of penhad1 were maintained only in the salt-r ...200516028118
[seasonal variation of tamarix ramosissima and populus euphratica water potentials in southern fringe of taklamakan desert].the measurement of the seasonal and diurnal variations of tamarix ramosissima and populus euphratica water potentials in the southern fringe of taklamakan desert indicated that there was no apparent water stress for the two species during their growth period, with little change of predawn water potential and some extent decrease of midday water potential. irrigation once or thinning had no significant effects on the water status of the plants, while groundwater appeared to be a prerequisite for ...200516262046
degradation of populus euphratica community in the lower reaches of the tarim river, xinjiang, china.to investigate the relationships between the degradation of plant community and groundwater level in the lower reaches of the tarim river, nine monitored sections were set along the main stream, where there had been no runoff for nearly 30 years. the characteristics of plant communities were analyzed. it was found that the coverage of trees gradually decreased along the groundwater depth gradient, while the coverage of shrubs slightly increased rather than decreased at first and then gradually d ...200516312995
[geography and host distribution of crimean-congo hemorrhagic fever in the tarim basin].to determine the infective status and natural distribution of xinjiang hemorrhagic fever (xhf; crimean-congo hemorrhagic fever, cchf) in ticks, rodents and livestock in the tarim basin.200617415983
proteome profiling of populus euphratica oliv. upon heat stress.populus euphratica is a light-demanding species ecologically characterized as a pioneer. it grows in shelter belts along riversides, being part of the natural desert forest ecosystems in china and middle eastern countries. it is able to survive extreme temperatures, drought and salt stress, marking itself out as an important plant species to study the mechanisms responsible for survival of woody plants under heat stress.200616740589
[function of the putative na+/h+ antiporter gene penhad1 from salt-resistant populus euphratica oliv].yeast complementation experiments were carried out to define the possible function of penhad1, a na(+)/h(+) antiporter gene from populus euphratica oliv., a salt resistant tree. penhad1 was introduced to the saccharomyces cerevisiae mutant strain ant3 (deltaena1-4::his3 deltanha1::leu2), which lacks the plasma membrane na(+)/h(+) antiporter gene scnha1 or gx1 (deltanhx1::trp1), which lacks tonoplast na(+)/h(+) antiporter gene scnhx1. our results showed that penhad1 rescued the normal growth of a ...200717452804
molecular characterization of pesos1: the putative na(+)/h (+) antiporter of populus euphratica.populus euphratica is a salt-tolerant tree species growing in semi-arid saline areas. a na(+)/h(+) antiporter gene was successfully isolated from this species through race cloning, and named pesos1. the isolated cdna was 3665 bp long and contained a 3438 bp open reading frame that was predicted to encode a 127-kda protein with 12 hypothetical transmembrane domains in the n-terminal part and a long hydrophilic cytoplasmic tail in the c-terminal part. the amino acid sequence of this pesos1 gene sh ...200717605111
ionic homeostasis and reactive oxygen species control in leaves and xylem sap of two poplars subjected to nacl stress.we investigated the effects of increasing soil nacl concentration on intracellular compartmentalization of salt and on the activities of antioxidant enzymes (superoxide dismutase (sod), ascorbic peroxidase (apx), catalase (cat) and glutathione reductase (gr)) and their role in the regulation of reactive oxygen species (ros; o(2)(-*) and h(2)o(2)) in leaves and xylem sap of salt-tolerant populus euphratica oliv. and salt-sensitive p. popularis cv. 35-44. mesophyll cells of p. euphratica exhibited ...200818381275
stress responsive zinc-finger protein gene of populus euphratica in tobacco enhances salt tolerance.the populus euphratica stress responsive zinc-finger protein gene pstz, which encodes a protein including typical cys(2)/his(2) zinc finger structure, was isolated by reverse transcription-polymerase chain reaction from p. euphratica. northern hybridization revealed that its expression was induced under drought and salt stress conditions. to examine its function, cdna of the pstz gene, driven by the cauliflower mosaic virus 35s promoter, was cloned into a plant expression vector pbin438 and intr ...200818666952
age structure and growth of degraded populus euphratica floodplain forests in north-west china and perspectives for their recovery.the populus euphratica floodplain forests along the lower reaches of the tarim river in continental-arid xinjiang (north-west china) have been declining in recent decades as a result of dam constructions and the withdrawal of water from the river. since 2000, controlled flooding has been carried out to counteract this process of degradation. however, the consequences of a changing water regime for the floodplain ecosystem are not yet sufficiently investigated. in 2004 we sampled a total of 150 p ...200818713421
[dynamic changes of groundwater level and vegetation in water table fluctuant belt in lower reaches of heihe river: coupling simulation].based on the 2006 investigation data in lower reaches of heihe river, and by using logarithmic normal distribution model, the models about the vegetation cover of populus euphratica and tamarix ramossima and the groundwater level in study area were built, and the potential plant of the study area was simulated. the results showed that in the lower reaches of heihe river, the optimal groundwater level and mean groundwater level for p. euphratica were 2.6 m and 3.6 m, and those for t. ramossima we ...200819288724
parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum bacteroidetes isolated from a forest soil.a novel golden-yellow-coloured strain, designated rhyl-37(t), was isolated from a soil sample from a forest of euphrates poplar (populus euphratica) in xinjiang, china. the cells were strictly aerobic, gram-staining-negative, heterotrophic, non-spore-forming rods that were motile by gliding. growth occurred at 17-37 degrees c (optimum, 30 degrees c), at ph 5.0-9.0 (optimum, ph 7.0) and with 0-1 % nacl (w/v; optimum, 0 %). phylogenetic analysis based on 16s rrna gene sequences indicated that the ...200919643901
expression profiling and functional characterization of a dreb2-type gene from populus euphratica.a novel dreb (dehydration responsive element binding) gene, designated pedreb2, was isolated from the desert-grown tree, populus euphratica. based on multiple sequence alignment and phylogenetic characterization, pedreb2 was classified as an a-2 group member of the dreb family. expression of pedreb2 was induced by cold, drought, and high salinity, but not by abscisic acid (aba) treatment. pedreb2 could bind specifically to dre elements and was targeted to the nucleus when transiently expressed i ...200919032934
molecular characterization of putative vacuolar nhx-type na(+)/h(+) exchanger genes from the salt-resistant tree populus euphratica.the vacuolar nhx-type na(+)/h(+) exchangers play a key role in salt tolerance in plants. however, little is known about the na(+)/h(+) exchangers in the salt-resistant tree, populus euphratica. in this study, we identified six putative vacuolar na(+)/h(+) exchanger genes from p. euphratica, designated as penhx1-6. real-time polymerase chain reaction indicated that the penhx1/3/6 transcripts were abundant compared with the other three penhx genes in the three tissues (roots, stems and leaves) exa ...200919678897
[water physiological characteristics of four perennial plant species around cele oasis in xinjiang].the water physiological characteristics of four perennial plant species (populus euphratica, tamarix ramosissima, calligonum caput-medusae, and alhagi sparsifolia) around cele oasis in xinjiang were studied through their vegetation growth period. no signs of serious drought stress were observed in any of the test perennial species during their vegetation growth, and irrigation had little effects on the water status of the plants (p > 0.05). the seasonal variation characteristics of water physiol ...200920135993
clonal structure and genetic diversity of three desert phreatophytes.the objective of this paper was to assess clone sizes of three perennial desert plant species with aflp markers and to relate them to clonal and genetic diversity and to hydroecology. the study was carried out at the southern rim of the taklamakan desert, where sexual regeneration is only possible shortly after rare flooding events, resulting in rarely established cohorts with subsequent extensive vertical growth and horizontal clonal spread. in this environment, repeated seedling establishment ...201021622383
linking the salt transcriptome with physiological responses of a salt-resistant populus species as a strategy to identify genes important for stress acclimation.to investigate early salt acclimation mechanisms in a salt-tolerant poplar species (populus euphratica), the kinetics of molecular, metabolic, and physiological changes during a 24-h salt exposure were measured. three distinct phases of salt stress were identified by analyses of the osmotic pressure and the shoot water potential: dehydration, salt accumulation, and osmotic restoration associated with ionic stress. the duration and intensity of these phases differed between leaves and roots. tran ...201020959419
cohnella luojiensis sp. nov., isolated from soil of a euphrates poplar forest.a novel strain, hy-22r(t), was isolated from soil of a euphrates poplar forest in xinjiang, china. the cells were gram-positive-staining, rod-shaped and motile by means of peritrichous flagella. growth occurred at 10-37 degrees c (optimum 30 degrees c), at ph 7.0-8.0 (optimum ph 7.0) and with 0-1 % nacl. a phylogenetic analysis based on 16s rrna gene sequences revealed that strain hy-22r(t) was closely related to cohnella phaseoli gspc1(t) (96.3 % sequence similarity). the major respiratory quin ...201019700453
sphingobacterium shayense sp. nov., isolated from forest soil.a gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated hs39(t), isolated from a soil sample collected from a natural populus euphratica forest in xinjiang, china, was characterized using a polyphasic approach. the isolate grew optimally at 30-37 °c, at ph 6.5-8.0 and with 0-3 % nacl. analysis of the 16s rrna gene sequence of strain hs39(t) revealed that it is a member of the genus sphingobacterium. sphingobacterium mizutaii ...201019933581
[correlationships between the coverage of vegetation and the quality of groundwater in the lower reaches of the tarim river].the variations vegetation coverage is the result of conjunct effects of inner and outer energy of the earth, however, the human activity always makes the coverage of vegetation change a lot. based on the monitoring data of chemistry of groundwater and the coverage of vegetation from 2002 to 2007 in the lower reaches of tarim river, relations between vegetation coverage and groundwater chemistry were studied. it is found that vegetation coverage at sector a was more than 80%, and decreased from s ...201020358816
shaker-like potassium channels in populus, regulated by the cbl-cipk signal transduction pathway, increase tolerance to low-k+ stress.shaker-like potassium channels in plants play an important role in potassium absorption and transport. here, we characterized 11 genes encoding shaker-like channels from populus trichocarpa. furthermore, two homologs from this family were isolated from populus euphratica and named pekc1 and pekc2. subcellular localization analysis of them in nicotiana benthamiana revealed that they are located in the cell membrane. yeast two-hybrid assays showed that they not only interacted strongly with pecipk ...201020582419
flavitalea populi gen. nov., sp. nov., isolated from soil of euphrates poplar (populus euphratica) forest in china.a novel strain, designated hy-50rt, isolated from soil of euphrates poplar (populus euphratica) forest in xinjiang, china, was characterized using a polyphasic taxonomic approach. the isolate was gram-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. phylogenetic analysis based on 16s rrna gene sequences showed that the isolate is a member of the phylum bacteroidetes, with niastella populi thyl-44t (93.6 % similarity), flavisolibacter ginsengisoli gsoil ...201020656815
seasonal quality profile and production of foliage from trees grown on degraded cropland in arid uzbekistan, central asia.feed shortages hamper livestock rearing and thus impede the development of rural livelihoods in central asia. the production and in vitro quality of foliage from ulmus pumila, elaeagnus angustifolia and populus euphratica on degraded cropland were examined to determine the potential of these species to supplement diary cattle diets. leaf dry matter (dm) production of the species, respectively, averaged 6, 8 and 17 t dm/ha, 4 years after planting. over seasons and years, crude protein concentrati ...201020662958
niastella populi sp. nov., isolated from soil of euphrates poplar (populus euphratica) forest, and emended description of the genus niastella.a novel bacterial strain, designated thyl-44(t), was isolated from the soil of a euphrates poplar (populus euphratica) forest in xinjiang, china. the cells were strictly aerobic, gram-staining-negative, non-flagellated, non-motile and filamentous. growth occurred at 17-37 degrees c (optimum 30 degrees c), at ph 5.0-8.0 (optimum ph 7.0) and with 0-1 % nacl (w/v; optimum 0 %). flexirubin pigments were not produced. a phylogenetic analysis based on 16s rrna gene sequences revealed that strain thyl- ...201019654358
pontibacter populi sp. nov., isolated from the soil of a euphrates poplar (populus euphratica) forest.a gram-negative, rod-shaped, non-motile and pink bacterial strain, designated strain hyl7-15t, was isolated from soil of a populus euphratica forest located in xinjiang, china, and was subjected to a polyphasic taxonomic analysis. 16s rrna gene sequences indicated that the isolate belonged to the phylum bacteroidetes and was related to the genus pontibacter, with sequence similarities ranging from 93.1 to 95.0 % with other pontibacter species. strain hyl7-15t contained mk-7 as the predominant me ...201121551332
[phylogenetic diversity of culturable endophytic bacteria isolated from the populus euphratica at the disused ancient ugan river].the aim of this study was to identify the culturable endophytic bacteria recovered from the populus euphratica at the disused (122 years ago) ancient ugan river of middle reachs of tarim river, and to understand their phylogenetic diversity and community structure.201121574378
[functional divergence of betaine aldehyde dehydrogenase genes in populus euphratica].plant betaine aldehyde dehydrogenase (badh) is a physiologically important enzyme in response to salt or drought stress. in this study, two badh genes (pebadh1 and pebadh2) were cloned from populus euphratica. both pebadh1 and pebadh2 genes encode the proteins of 503 amino acid residues, with a calculated molecular mass of 54.93 kda and 54.90 kda, respectively. reverse transcription pcr showed the divergence of expression pattern between the pebadh1 and pebadh2 genes in p. euphratica. the recomb ...201222712391
improvement of drought and salt tolerance in arabidopsis and lotus corniculatus by overexpression of a novel dreb transcription factor from populus euphratica.a novel dreb (dehydration-responsive element binding) gene, designated pedreb2a, was isolated from the desert-grown tree, populus euphratica oliv. pedreb2a is classified into the a-5 group of dreb subfamily based on multiple sequence alignment and phylogenetic characterization. using semi-quantitative rt-pcr, we found that the pedreb2a was greatly induced by drought, nacl, low temperature, 1-naphthaleneacetic acid (naa), 6-benzyl aminopurine (6-ba) and gibberellic acid (ga3) treatments in p. eup ...201222771912
rhizobium tarimense sp. nov., isolated from soil in the ancient khiyik river.a gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain, pl-41(t), was isolated from populus euphratica forest soil at the ancient khiyik river valley in xinjiang uyghur autonomous region, people's republic of china. strain pl-41(t) grew optimally at 30 °c and ph 7.0-8.0. the major quinone was q-10. the predominant cellular fatty acids of strain pl-41(t) were summed feature 8 (comprising c18 : 1ω7c and c18 : 1ω6c), c16 : 0 and c19 : 0 cyclo ω8c. polar lipids of strain pl-41(t) incl ...201323203621
overexpression of peha1 enhances hydrogen peroxide signaling in salt-stressed arabidopsis.the plant plasma membrane (pm) h(+)-atpase plays a crucial role in controlling k(+)/na(+) homeostasis under salt stress. our previous microarray analysis indicated that populus euphratica retained a higher abundance of pm h(+)-atpase transcript versus a salt-sensitive poplar. to clarify the roles of the pm h(+)-atpase in salt sensing and adaptation, we isolated the pm h(+)-atpase gene peha1 from p. euphratica and introduced it into arabidopsis thaliana. compared to wild-type, peha1-transgenic ar ...201323872741
a populus euphratica nac protein regulating na⁺/k⁺ homeostasis improves salt tolerance in arabidopsis thaliana.nac transcription factors are plant-specific and play an important role in responses to biotic and abiotic stresses. populus euphratica is a salt-tolerant tree species growing in semi-arid saline areas. a stress responsive gene was successfully isolated from this species and named penac1. the isolated cdna encoded a protein containing a conserved nac domain that belonged to the ataf subgroup of the nac protein family. the protein was localized to the nucleus and its c-terminus had transcriptiona ...201323545306
the calcium sensor pecbl1, interacting with pecipk24/25 and pecipk26, regulates na(+)/k (+) homeostasis in populus euphratica.this paper is the first to directly link two types of ion channel regulation pathway into an emerging and complex cbl-cipk signal system in wooden plant. in arabidopsis thaliana, the calcineurin b-like (cbl) 1 gene has been shown to be necessary in response to abiotic stresses. in this study, we identified cbl1 in the woody plant populus euphratica, designated as pecbl1. heterologous expression of pecbl1 could build the resistance of sensitive phenotypes to low k(+) stress in the corresponding a ...201323423605
sphingobacterium hotanense sp. nov., isolated from soil of a populus euphratica forest, and emended descriptions of sphingobacterium daejeonense and sphingobacterium shayense.a novel gram-staining-negative bacterial strain, designated xh4(t), was isolated from soil of a populus euphratica forest in the hotan river valley, xinjiang uyghur autonomous region, pr china. the cells were strictly aerobic, non-motile, short rods. the isolate grew optimally at 37 °c and at ph 7.0-8.0. based on 16s rrna gene sequence analysis, strain xh4(t) belonged to the genus sphingobacterium and was closely related to sphingobacterium mizutaii atcc 33299(t) (96.1 % sequence similarity). th ...201322611196
desertibacter xinjiangensis sp. nov., isolated from the soil of a euphrates poplar forest, and emended description of the genus desertibacter.a pale pink and strictly aerobic bacterium, designated strain m71(t), was isolated from the soil of a euphrates poplar forest in xingjiang, pr china. cells of the strain were gram-reaction-negative, rod-shaped and motile by means of a single polar flagellum. growth occurred at 10-37 °c (optimum 30 °c), at ph 6.0-9.0 (optimum ph 7.0-8.0) and with 0-2.0% nacl (w/v, optimum 0%). phylogenetic analysis, based on 16s rrna gene sequences, indicated that strain m71(t) belongs to the genus desertibacter ...201425106921
rhizobium populi sp. nov., an endophytic bacterium isolated from populus euphratica.an endophytic bacterium, designated k-38(t), was isolated from the storage liquid in the stems of populus euphratica trees at the ancient ugan river in xinjiang, pr china. strain k-38(t) was found to be rod-shaped, gram-stain-negative, aerobic, non-motile and non-spore-forming. strain k-38(t) grew at temperatures of 25-37 °c (optimum, 28 °c), at ph 6.0-9.0 (optimum, ph 7.5) and in the presence of 0-3 % (w/v) nacl with 1 % as the optimum concentration for growth. according to phylogenetic analysi ...201424972613
pontibacter yuliensis sp. nov., isolated from soil.a gram-staining-negative, rod-shaped and pink bacterium was isolated from the soil of a populus euphratica forest in the taklamakan desert, xinjiang, china. it was designated strain h9x(t). a 16s rrna gene sequence homology search indicated that the isolate was most closely related to the family cytophagaceae. the 16s rrna gene of strain h9x(t) displayed 94.2-96.3 % sequence identities to those of type strains of other species of the genus pontibacter. it only possessed menaquinone-7. the major ...201424425811
cloning of the cryptochrome-encoding pecry1 gene from populus euphratica and functional analysis in arabidopsis.cryptochromes are photolyase-like blue/uv-a light receptors that evolved from photolyases. in plants, cryptochromes regulate various aspects of plant growth and development. despite of their involvement in the control of important plant traits, however, most studies on cryptochromes have focused on lower plants and herbaceous crops, and no data on cryptochrome function are available for forest trees. in this study, we isolated a cryptochrome gene, pecry1, from euphrates poplar (populus euphratic ...201425503486
temperature-induced lipocalin (til) is translocated under salt stress and protects chloroplasts from ion toxicity.temperature-induced lipocalins (til) have been invoked in the defense from heat, cold and oxidative stress. here we document a function of til for basal protection from salinity stress. heterologous expression of til from the salt resistant poplar populus euphratica did not rescue growth but prevented chlorophyll b destruction in salt-exposed arabidopsis thaliana. the protein was localized to the plasma membrane but was re-translocated to the symplast under salt stress. the a. thaliana knock out ...201424028869
functional analysis of the na+,k+/h+ antiporter penhx3 from the tree halophyte populus euphratica in yeast by model-guided mutagenesis.na+,k+/h+ antiporters are h+-coupled cotransporters that are crucial for cellular homeostasis. populus euphratica, a well-known tree halophyte, contains six na+/h+ antiporter genes (penhx1-6) that have been shown to function in salt tolerance. however, the catalytic mechanisms governing their ion transport remain largely unknown. using the crystal structure of the na+/h+ antiporter from the escherichia coli (ecnhaa) as a template, we built the three-dimensional structure of penhx3 from p. euphra ...201425093858
expression profiling and functional characterization of a cbl-interacting protein kinase gene from populus euphratica.this is the first report on the function of a member of the cipk family in populus euphratica. the ca(2+)-dependent salt overly sensitive (sos) pathway has been shown to play an essential role in maintaining ion homeostasis and conferring salt tolerance. one component of the sos pathway, sos1, was identified in the salt-resistant tree p. euphratica. in this study, we identified and functionally characterized another component of the sos pathway in this tree called pesos2 or pecipk26. on the basi ...201424413762
survey of plant drought-resistance promoting bacteria from populus euphratica tree living in arid area.two hundred and thirty-two bacterial strains were isolated from the rhizospheric soil of populus euphratica which is the dominant tree living in extreme arid regions in northwest china. some strains with plant growth-promoting bacteria related metabolic characteristics were able to promote drought resistance in plants after inoculation. ten strains with the greatest effects increased the dry weight of wheat shoots from 0.5 to 34.4 %, and the surface area of the root systems from 12.56 to 212.17  ...201425320440
high rates of virus-induced gene silencing by tobacco rattle virus in populus.virus-induced gene silencing (vigs) has been shown to be an effective tool for investigating gene functions in herbaceous plant species, but has rarely been tested in trees. the establishment of a fast and reliable transformation system is especially important for woody plants, many of which are recalcitrant to transformation. in this study, we established a tobacco rattle virus (trv)-based vigs system for two populus species, populus euphratica and p. × canescens. here, trv constructs carrying ...201526209619
[heat-responsive mechanisms in plants revealed by proteomic analysis: a review].heat stress is a major abiotic stress that limits plant growth and productivity. in recent years, proteomic investigations provide more information for understanding the sophisticated heat-responsive molecular mechanism in plants at systematic biological level. the heat-responsive proteomic patterns in several plants, i. e., model plants (arabidopsis thaliana), staple food crops (soybean, rice and wheat), heat-tolerant plants (agrostis stolonifera, portulaca oleracea, and carissa spinarum), grap ...201526685622
a putative pp2c-encoding gene negatively regulates aba signaling in populus euphratica.a pp2c homolog gene was cloned from the drought-treated cdna library of populus euphratica. multiple sequence alignment analysis suggested that the gene is a potential ortholog of hab1. the expression of this hab1 ortholog (pehab1) was markedly induced by drought and moderately induced by aba. to characterize its function in aba signaling, we generated transgenic arabidopsis thaliana plants overexpressing this gene. transgenic lines exhibited reduced responses to exogenous aba and reduced tolera ...201526431530
populus euphratica hsf binds the promoter of wrky1 to enhance salt tolerance.poplar species increase expressions of transcription factors to deal with salt environments. we assessed the salt-induced transcriptional responses of heat-shock transcription factor (hsf) and wrky1 in populus euphratica, and their roles in salt tolerance. high nacl (200mm) induced pehsf and pewrky1 expressions in p. euphratica, with a rapid rise in roots than in leaves. moreover, the salt-elicited pehsf reached its peak level 6h earlier than pewrky1 in leaves. pewrky1 was down-regulated in sali ...201525900569
correction: functional analysis of the na+,k+/h+ antiporter penhx3 from the tree halophyte populus euphratica in yeast by model-guided mutagenesis.[this corrects the article doi: 10.1371/journal.pone.0104147.].201525646763
populus euphratica apyrase2 enhances cold tolerance by modulating vesicular trafficking and extracellular atp in arabidopsis plants.apyrase and extracellular atp play crucial roles in mediating plant growth and defense responses. in the cold-tolerant poplar, populus euphratica, low temperatures up-regulate apyrase2 (peapy2) expression in callus cells. we investigated the biochemical characteristics of peapy2 and its role in cold tolerance. we found that peapy2 predominantly localized to the plasma membrane, but punctate signals also appeared in the endoplasmic reticulum and golgi apparatus. peapy2 exhibited broad substrate s ...201526224801
rhizobacteria of populus euphratica promoting plant growth against heavy metals.the heavy metal-resistant bacteria from rhizospheric soils of wild populus euphratica forest growing in arid and saline area of northwestern china were investigated by cultivation-dependent methods. after screening on medium sparked with zinc, copper, nickel and lead, 146 bacteria strains with different morphology were isolated and most of them were found to be resistant to at least two kinds of heavy metals. significant increase in fresh weight and leaf surface area of arabidopsis thaliana seed ...201526208544
molecular cloning and functional analysis of uv resistance locus 8 (peuvr8) from populus euphratica.ultraviolet-b (uv-b; 280-315 nm) light, which is an integral part of the solar radiation reaching the surface of the earth, induces a broad range of physiological responses in plants. the uv resistance locus 8 (uvr8) protein is the first and only light photoreceptor characterized to date that is specific for uv-b light and it regulates various aspects of plant growth and development in response to uv-b light. despite its involvement in the control of important plant traits, most studies on uv-b ...201526171608
[rhizospheria bacteria of poplus euphratica improve resistance of wood plants to heavy metals].populus euphratica is a special kind of woody plant, which lives in desert area of northwestern china and is strongly resistant to multiple abiotic stresses. however, the knowledge about the ecology and physiological roles of microbes associated with p. euphratica is still not enough. in this paper, we isolated 72 strains resistant to heavy metals from rhizospheric soil of wild p. euphratica forest in shaya county of xinjiang. there were 50 strains conveying resistance to one of four heavy metal ...201526785565
nesterenkonia populi sp. nov., an actinobacterium isolated from populus euphratica.an alkaliphilic and moderately halophilic actinobacterium, designated strain gp10-3(t), was isolated from populus euphratica collected from the southern edge of taklimakan desert, xinjiang, china. cells of this strain were gram-stain-positive, non-motile and non-spore-forming short rods. strain gp10-3(t) grew optimally at 37 °c on lb agar media in the presence of 5-10% (w/v) nacl at ph 9.0. the menaquinones were mk-7, mk-8 and mk-9. the major fatty acids (>10%) were anteiso-c17 : 0, anteiso-c15 ...201525701851
populus euphratica: an incompatible host for biotrophic pathogens? 201627492503
streptomyces indoligenes sp. nov., isolated from rhizosphere soil of populus euphratica.a novel actinobacterium, designated trm 43006t, was isolated from the rhizosphere soil of populus euphratica in xinjiang province, north-west china. phylogenetic and phenotypic analysis demonstrated that strain trm 43006t belongs to the genus streptomyces. the strain was aerobic and gram-stain-positive; the aerial mycelium branched monopodially, forming chains of arthrospores. the spores were oval to cylindrical with smooth surfaces. the whole-cell sugar pattern of strain trm 43006t consisted of ...201627031169
functional analyses of populus euphratica brassinosteroid biosynthesis enzyme genes dwf4 (pedwf4) and cpd (pecpd) in the regulation of growth and development of arabidopsis thaliana.dwf4 and cpd are key brassinosteroids (brs) biosynthesis enzyme genes. to explore the function of populus euphratica dwf4 (pedwf4) and cpd (pecpd), arabidopsis thaliana transgenic lines (tls) expressing pedwf4, pecpd or pedwf4 plus pecpd, namely pedwf4-tl, pecpd-tl and pecp/dw-tl, were characterized. compared with wild type (wt), the changes of both pedwf4-tl and pecpd-tl in plant heights, silique and hypocotyls lengths and seed yields were similar, but in bolting time and stem diameters, they w ...201627966492
isolation and functional characterization of a novel gene encoding a dehydration responsive element binding transcription factor from populus euphratica.dehydration responsive element binding (dreb) transcription factors (tfs) play a key role in regulating abiotic stress related genes. a new gene (pedreb2b) encoding an unidentified dre-binding protein was isolated from 20% peg6000 treated populus euphratica oliv. seedlings by rt-pcr and race. full length of pedreb2b cdna was 1110 bp, and an orf of 870 bp, which encoded 289-amino-acids polypeptide, were included. the deduced amino acid sequence analysis revealed that this protein was a putative a ...201627001404
simultaneous overexpression of the hherf2 and pedreb2a genes enhanced tolerances to salt and drought in transgenic cotton.a desert-grown halimodendron halodendron ethylene-responsive element binding factor gene (hherf2), which encodes a 245 amino acids protein containing a conserved ap2/erebp domain, was isolated through the rapid amplification cdna end (race) method. sequence and phylogenetic analysis indicated that hherf2 was classified into the b-2 group of erf subfamily. semiquantitative rt-pcr showed that hherf2 was greatly induced by treatments with high-salt, drought and low temperature in h. halodendron see ...201626972971
pseudomonas populi sp. nov., an endophytic bacterium isolated from populus euphratica.a gram-stain negative, aerobic, non-motile, short rod-shaped bacterium, designated kbl-4-9t, was isolated from the storage liquid in the stems of populus euphratica trees in xinjiang, pr china. strain kbl-4-9t grew at 4-45 ℃ (optimum 37 ℃), 1-3 % (w/v) nacl (optimum 1 %, w/v) and ph 5.5-9.5 (optimum ph 7.5). phylogentic analyses based on 16s rrna gene sequences indicated that strain kbl-4-9t belonged to the genus pseudomonas and showed the highest 16s rrna gene sequence similarity of 97.36 % to ...201626827750
identification and functional characterization of the nac gene promoter from populus euphratica.the penac1 promoter is a non-tissue-specific and stress-inducible promoter containing a ga-responsive element and a myb recognition sequence that are responsible for induced expression patterns. nac transcription factors play vital roles in complex signaling networks during plant stress responses. promoters as crucial molecular switches are involved in the transcriptional regulation of gene activities dynamic network controlling a variety of biological processes, such as developmental processes, ...201627084679
low nitrogen stress stimulating the indole-3-acetic acid biosynthesis of serratia sp. zm is vital for the survival of the bacterium and its plant growth-promoting characteristic.serratia sp. zm is a plant growth-promoting (pgp) bacterial strain isolated from the rhizospheric soil of populus euphratica in northwestern china. in this study, low nitrogen supply significantly stimulated the production of indole-3-acetic acid (iaa) in serratia sp.zm. the inoculation of the bacterium to wheat seedlings improved plant growth compared with the uninoculated group, and the stimulating effect was more prominent under low nitrogen stress. inactivation of the predicted key gene in t ...201727803972
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