| herpes-like virus detection in infected crassostrea gigas spat using dig-labelled probes. | an in situ hybridization protocol for detecting the herpes-like virus which infects french pacific oysters, crassostrea gigas, was developed. two dna probes were synthesized by incorporation of digoxigenin 11-dutp during pcr. two oyster herpes-like virus specific primer pairs, a5/a6 and c1/c6, were used. both dig-labelled probes were able to detect 50 pg of herpes-like virus pcr amplified dna in southern blot hybridizations. the probes hybridized with viral dna in paraffin sections of infected c ... | 2002 | 11849678 |
| development of a pcr procedure for the detection of a herpes-like virus infecting oysters in france. | a pcr-based procedure for detecting a herpes-like virus that infects the japanese oyster, crassostrea gigas, in france was developed. two primers were designed to provide specific amplification products ranging in size from 917 to 1001 bp when carried out on oyster herpes-like virus dna. no amplification was observed of oyster genomic dna nor of the dna from vertebrate herpesviruses. crude samples were prepared and submitted to nested pcr, allowing amplification of dna fragments of the expected ... | 2000 | 10921841 |