multiplex reverse transcription-pcr for simultaneous detection of beet necrotic yellow vein virus, beet soilborne virus, and beet virus q and their vector polymyxa betae keskin on sugar beet.three soilborne viruses transmitted by polymyxa betae keskin in sugar beet have been described: beet necrotic yellow vein virus (bnyvv), the agent of rhizomania, beet soilborne virus (bsbv), and beet virus q (bvq). a multiplex reverse transcription-pcr technique was developed to simultaneously detect bnyvv, bsbv, and bvq, together with their vector, p. betae. the detection threshold of the test was up to 128 times greater than that of an enzyme-linked immunosorbent assay. systematic association ...200312676720
new bnyvv p25 variants in belgium.rhizomania is a widespread viral plant disease of major importance in sugar beet cropping and breeding. it is caused by the beet necrotic yellow vein virus (bnyvv), a benyvirus transmitted by the soil inhabiting plasmodiophorid polymyxa betae. this vector also transmits other sugar beet virus such as beet virus q (bvq) and beet soil-borne virus (bsbv). despite identification of resistance genes, bnyvv remains a major constraint because of resistance-breaking events as well as its ability to surv ...201527141738
the beet virus q coat protein readthrough domain is longer than previously reported, with two transmembrane domains.ten beet virus q (bvq) strains from six different countries were sequenced to characterize the readthrough (rt) domain of the coat protein (cp). the genbank/embl/ddbj accession numbers for the sequences reported in this paper are fm244643-fm244652. with three nucleotide additions of 5, 285 and 1 nt, the common rt of 76 kda was found to be longer than the single reference available to date (35 kda). it is hypothesized that multiple inoculation cycles on chenopodium quinoa were responsible for the ...200919218222
efficient dsrna-mediated transgenic resistance to beet necrotic yellow vein virus in sugar beets is not affected by other soilborne and aphid-transmitted viruses.rhizomania caused by beet necrotic yellow vein virus (bnyvv) is one of the most devastating sugar beet diseases. sugar beet plants engineered to express a 0.4 kb inverted repeat construct based on the bnyvv replicase gene accumulated the transgene mrna to similar levels in leaves and roots, whereas accumulation of the transgene-homologous sirna was more pronounced in roots. the roots expressed high levels of resistance to bnyvv transmitted by the vector, polymyxa betae. resistance to bnyvv was n ...200817431806
genome properties of beet virus q, a new furo-like virus from sugarbeet, determined from unpurified virus.based solely on the information that beet virus q (bvq) contains tubular particles, the entire nucleotide sequence of its tripartite genome was determined from unpurified virus in ca. 40 ml crude sap from locally infected chenopodium quinoa. a starting sequence for rna 1 was generated using primers corresponding to highly conserved helicase domains in the respective rnas of furo-, pomo-, peclu-, hordei- and tobraviruses, and was extended by a walking random-primed cdna approach. the similarity o ...19989714254
the beet soilborne pomovirus in belgium and relationship with rhizomania.the objective of this study was to determine the extend of the beet soilborne pomovirus (bsbv) and the beet virus q in sugar beet fields in belgium. during the 2000 sugar beet growing season, more than 80 fields located in belgium were investigated for the presence of the beet necrotic yellow vein benyvirus (bnyvv), the bsbv and polymyxa betae, the plasmodiophorid vector of both viruses. fields investigated were identified either using previous recorded data or by visual identification of yellow ...200112425019
sequence divergence of four soilborne sugarbeet-infecting viruses.soilborne viruses are among the most harmful pathogens of sugarbeet (beta vulgaris l.ssp. vulgaris) but most of them lack information on genetic variability due to paucity of sequence data. only one isolate of beet soil borne virus (bsbv; genus pomovirus), beet virus q (bvq; genus pomovirus) and beet soil borne mosaic virus (bsbmv; genus benyvirus) has been characterised for the coat protein (cp) gene. in this study, the cp gene sequences of three isolates each of bsbv and beet necrotic yellow v ...200515965609
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