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nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of nitriliruptoraceae fam. nov. and nitriliruptorales ord. nov.a novel bacterial strain, designated anl-iso2(t), was obtained from an enrichment culture inoculated with a mixture of soda lake sediments by using isobutyronitrile (ibn) as the carbon, energy and nitrogen source at ph 10. the enrichment resulted in a stable binary culture containing ibn-degrading gram-positive rods and a satellite gram-negative gammaproteobacterium marinospirillum sp. strain (anl-isoa) scavenging the products of nitrile hydrolysis. cells of the ibn-degrading strain, anl-iso2(t) ...200919196761
isolation and characterisation of bacteria from the haloalkaline lake elmenteita, kenya.culture-independent studies show that soda lake environments harbour diverse groups of bacteria and archaea. in this study different enrichment and isolation media were used in an attempt to isolate novel groups of bacteria from lake elmenteita. different media were prepared using filter-sterilised water from the lake. the isolates recovered were purified on tryptic soy agar supplemented with 1% sodium carbonate and 4% sodium chloride. phylogenetic analysis of 181 partial 16s rrna gene sequences ...201020401502
marinospirillum celere sp. nov., a novel haloalkaliphilic, helical bacterium isolated from mono lake.two strains of a gram-negative, helical, haloalkaliphilic bacterium were isolated from mono lake (usa). both strains were mesophilic and grew between 13 and 55 degrees c, with optimum growth at 35-45 degrees c. the optimum ph for growth was 9.5. growth was observed at nacl concentrations of 0.5-12% (w/v), with optimum growth at 2% nacl. both isolates were motile by means of bipolar tuft flagella, coccoid body-forming and strictly aerobic. it was concluded that they belong to the same species, ba ...200919620364
microbial isobutyronitrile utilization under haloalkaline conditions.the utilization of isobutyronitrile (ibn) as a c and n source under haloalkaline conditions by microbial communities from soda lake sediments and soda soils was studied. in both cases, a consortium consisting of two different bacterial species capable of the complete degradation and utilization of ibn at ph 10 was selected. the soda lake sediment consortium consisted of a new actinobacterium and a gammaproteobacterium from the genus marinospirillum. the former was capable of fast hydrolysis of a ...200717644641
marinospirillum insulare sp. nov., a novel halophilic helical bacterium isolated from kusaya gravy.a novel species that belongs to the genus marinospirillum is described on the basis of phenotypic characteristics, phylogenetic analysis of 16s rrna and gyrb gene sequences and dna-dna hybridization. four strains of helical, halophilic, gram-negative, heterotrophic bacteria were isolated from kusaya gravy, which is fermented brine that is used for the production of traditional dried fish in the izu islands of japan. all of the new isolates were motile by means of bipolar tuft flagella, of small ...200414742475
saccharospirillum impatiens gen. nov., sp. nov., a novel gamma-proteobacterium isolated from hypersaline ekho lake (east antarctica).five gram-negative, motile, aerobic to microaerophilic spirilla were isolated from various depths of the hypersaline, heliothermal and meromictic ekho lake (east antarctica). the strains are oxidase- and catalase-positive, metabolize a variety of sugars and carboxylic acids and have an absolute requirement for sodium ions. the predominant fatty acids of the organisms are c(16 : 1)omega7c, c(16 : 0) and c(18 : 1)omega7c, with c(10 : 1))3-oh, c(10 : 0) 3-oh, c(12 : 0) 3-oh, c(14 : 1)3-oh, c(14 : 0 ...200312807182
marinospirillum alkaliphilum sp. nov., a new alkaliphilic helical bacterium from haoji soda lake in inner mongolia autonomous region of china.a new helical, alkaliphilic, gram-negative, chemoorganotrophic bacterium designated strain z4t was isolated from haoji soda lake in inner mongolia autonomous region, china. the isolate grows at salinities between 0.2% and 5.0% (w/v) nacl and ph range 7.0-11.0, with an optimum at 2.0% (w/v) nacl and ph 9.5. its growth temperature ranges from 8 degrees to 49 degrees c with an optimum at 37 degrees c. the g+c content of the dna is 46.8 mol%. the major isoprenoid quinone is ubiquinone 8 (q-8). phylo ...200211878559
artificial neural network based identification of environmental bacteria by gas-chromatographic and electrophoretic data.chemotaxonomic identification techniques are powerful tools for environmental micro-organisms, for which poor diagnostic schemes are available. whole cellular fatty acid methyl esters (fame) content is a stable bacterial profile, the analysis method is rapid, cheap, simple to perform and highly automated. whole-cell protein is an even more powerful tool because it yields information at or below the species level. the description of new species and genera and subsequent continuous rearrangement p ...200011084227
marinospirillum gen. nov., with descriptions of marinospirillum megaterium sp. nov., isolated from kusaya gravy, and transfer of oceanospirillum minutulum to marinospirillum minutulum comb. nov.two strains of helical, halophilic, gram-negative, heterotrophic bacteria were isolated from kusaya gravy which is a traditional japanese fermented brine. these strains were motile by means of a single polar or bipolar tuft flagellum. they had a large cell size, were helical, formed coccoid bodies, were microaerophilic and had quinone type q-8. the dna g + c content of the strains was 44-45 mol %. a detailed investigation of the phenotypic, chemotaxonomic and phylogenetic characteristics of the ...19989828435
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