a plausible role for pollen-residing molds in agricultural purposes.pollen microbial content of 15 samples was investigated. pollen was collected by honeybees. total aerobic mesophilic count ranged from 3.00 to 5.48 log cfu/g. total mold and yeast count ranged from 2.3 to 6.99 log cfu/g. selected strains of isolated molds from pollen samples were characterized by conventional methods. potent phytopathogenic and food spoilage species such as penicillium sp., alternaria alternata, alternaria sp., cladosporium werneckii, mucor hiemalis, rhizomucor pusillus, aspergi ...201527141752
application of a multiplex suspension array for rapid and simultaneous identification of clinically important mold pathogens.we have developed a microsphere-based suspension array (msa) for the identification of 23 medically important mold pathogens including aspergillus spp., fusarium spp., mucor spp., rhizopus spp., rhizomucor pusillus, penicillium marneffei, saksenaea vasiformis, apophysomyces elegans, lichtheimia corymbifer, and syncephalastrum racemosum. twenty-one oligonucleotide probes were designed based on the internal transcribed spacer (its2) region for species level identification of molds. among the 21 pr ...201222771862
growth of chitinolytic dune soil beta-subclass proteobacteria in response to invading fungal has frequently been reported that chitinolytic soil bacteria, in particular biocontrol strains, can lyse living fungal hyphae, thereby releasing potential growth substrate. however, the conditions used in such assays (high bacterial density, rich media, fragmented hyphae) make it difficult to determine whether mycolytic activity is actually of importance for the growth and survival of chitinolytic bacteria in soils. an unidentified group of beta-subclass proteobacteria (cbetaps) was most domi ...200111472904
purification and characterisation of an acid phosphatase with phytase activity from mucor hiemalis acid phosphatase with phytase activity, produced by mucor hiemalis wehmer, was purified to homogeneity by a combination of anion exchange, gel filtration and hydrophobic interaction chromatography. the monomeric, glycosylated enzyme displayed maximum activity at 55 degrees c and ph 5.0-5.5. when compared to commercialised products, the enzyme is more thermostable (80 degrees c, 5min), displays a broader ph versus activity profile and greater stability under simulated digestive tract condition ...200717889394
efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar efficient method for synthesizing homogenous glycoproteins is essential for elucidating the structural and functional roles of glycans of glycoproteins. we have focused on the transglycosylation activity of endo-ß-n-acetylglucosaminidase from mucor hiemalis (endo-m) as a tool for glycoconjugate syntheses, since it can transfer en bloc the oligosaccharide of not only high-mannose type but also complex-type n-glycan onto various acceptors having an n-acetylglucosamine residue. however, there ar ...201020647032
chemoenzymatic synthesis and application of a sialoglycopolymer with a chitosan backbone as a potent inhibitor of human influenza virus hemagglutination.sialoglycopeptide (sgp) is referred as the glycopeptide in hen's egg yolk, which has an n-linked, complex-type, disialyl biantennary oligosaccharide with an alpha-(2-->6)-sialyl n-acetyllactosamine residue. the residue is known as a binding ligand of type-a human influenza virus hemagglutinin. we describe herein a simple synthesis of a sialoglycopolymer with a chitosan backbone as a potent inhibitor of human influenza virus hemagglutination that makes use of the natural source ingredient, sgp, a ...200616716273
biodegradation by bioaugmentation of dairy wastewater by fungal consortium on a bioreactor lab-scale and on a pilot-scale.a fungal consortium including aspergillus niger, mucor hiemalis and galactomyces geotrichum was tested for the treatment of dairy wastewater. the bio-augmentation method was tested at lab-scale (4 l), at pilot scale (110 l) and at an industrial scale in wastewater treatment plants (wwtp). the positive impact of fungal addition was confirmed when fungi was beforehand accelerated by pre-culture on whey (5 g/l lactose) or on the dairy effluent. indeed, chemical oxygen demand (cod) removal yields in ...201324520735
a new mercury-accumulating mucor hiemalis strain eh8 from cold sulfidic spring water, we report about a unique aquatic fungus mucor hiemaliseh8 that can remove toxic ionic mercury from water by intracellular accumulation and reduction into elemental mercury (hg(0) ). eh8 was isolated from a microbial biofilm grown in sulfidic-reducing spring water sourced at a marching's site located downhill from hop cultivation areas with a history of mercury use. a thorough biodiversity survey and mercury-removal function analyses were undertaken in an area of about 200 km(2) in bavaria ...201627177603
[the effect of hydrogen peroxide on the growth of microscopic mycelial fungi isolated from habitats with different levels of radioactive contamination].the effect of hydrogen peroxide ( 10(-9)-10(-1) m) on the mycelial growth of the fungi alternaria alternata, cladosporium cladosporioides, mucor hiemalis, and paecilomyces lilacinus has been studied. the growth of fungi isolated from habitats with a background level of radioactive contamination was stopped by h2o2 concentrations equal to 10(-3) and 10(-2) m, whereas the growth of fungi that were isolated from habitats with high levels of radioactive contamination was only arrested by 10(-1) m h2 ...200516400985
comparison of antifungal activities of vietnamese citrus essential oils.citrus essential oils (eos) are volatile compounds from citrus peels and widely used in perfumes, cosmetics, soaps and aromatherapy. in this study, inhibition of citrus eos extracted from vietnamese orange (citrus sinensis), mandarin (citrus reticulata blanco), pomelo (citrus grandis osbeck) and lime (citrus aurantifolia swingle) on the growth of plant pathogenic fungi, mucor hiemalis, penicillium expansum and fusarium proliferatum was investigated. the eos of the citrus peels were obtained by c ...201322799453
influence of autoclaved saprotrophic fungal mycelia on proteolytic activity in ectomycorrhizal fungi.the production of proteolytic enzymes by several strains of ectomycorrhizal fungi i.e., amanita muscaria (16-3), laccaria laccata (9-12), l. laccata (9-1), suillus bovinus (15-4), suillus bovinus (15-3), suillus luteus (14-7) on mycelia of trichoderma harzianum, trichoderma virens and mucor hiemalis and sodium caseinate, yeast extract was evaluated. the strains of a. muscaria (16-3) and l. laccata (9-12) were characterized by the highest activity of the acidic and neutral proteases. taking the m ...200717333458
plant community influences on soil microfungal assemblages in boreal mixed-wood forests.we studied the relationships between assemblages of soil microfungi and plant communities in the southern boreal mixed-wood forests of quebec. sampling took place in 18,100 m2 plots from an existing research site. plots were separated into three categories based on dominant overstory tree species: (i) trembling aspen, (ii) white birch and (iii) a mixture of white spruce and balsam fir. within each plot a 1 m2 subplot was established in which the understory herbaceous layer was surveyed and soil ...200717883027
discovery and development of the epothilones : a novel class of antineoplastic drugs.the epothilones are a novel class of antineoplastic agents possessing antitubulin activity. the compounds were originally identified as secondary metabolites produced by the soil-dwelling myxobacterium sorangium cellulosum. two major compounds, epothilone a and epothilone b, were purified from the s. cellulosum strain so ce90 and their structures were identified as 16-member macrolides. initial screening with these compounds revealed a very narrow and selective antifungal activity against the zy ...200818095749
mucor nidicola sp. nov., a novel fungal species isolated from an invasive paper wasp nest.mucor nidicola sp. nov. is a novel mucoralean fungus isolated from a nest of the invasive paper wasp, polistes dominulus. phylogenetic analysis based on the internal transcribed spacers (its) and 5.8s rrna gene sequences, along with physiological tests, revealed that this is a species within the genus mucor. the new species also includes a representative that had previously been characterized as part of the m. hiemalis complex. unlike the type strain of m. hiemalis, these two strains can grow at ...201121930681
induction of glutathione s-transferase in biofilms and germinating spores of mucor hiemalis strain eh5 from cold sulfidic spring waters.the occurrence and activation of glutathione s-transferase (gst) and the gst activities in biofilms in cold sulfidic spring waters were compared to the occurrence and activation of gst and the gst activities of the aquatic fungal strains eh5 and eh7 of mucor hiemalis isolated for the first time from such waters. using fluorescently labeled polyclonal anti-gst antibodies and gst activity measurements, we demonstrated that a high level of gst occurred in situ in natural biofilms and pure cultures ...200717293503
resolution of oligosaccharides in glycopeptides using immobilized endo-m and ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.the resolution of asparagine-linked oligosaccharides in glycopeptides was carried out by combination of the transglycosylation reaction and ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry (uplc-esi-tof-ms). the resolution of the oligosaccharides is based on the enzymic transglycosylation reaction with endo-beta-n-acetylglucosaminidase (endo-m) isolated from mucor hiemalis. the oligosaccharides were transferred to a fluorescent acceptor (nda-a ...200717441218
mycoremediation-a prospective environmental friendly technique of bioseparation and dewatering of domestic wastewater sludge.introduction: environmental safe and friendly management and disposal of wastewater sludge is a problem of every treatment plant throughout the world. bioseparation and dewaterability of raw domestic wastewater sludge were evaluated for proper management and disposal by mycoremediation, i.e., using prior grown 2% (v/v) spore suspension of filamentous fungal (mucor hiemalis wehmer) broth inoculation, which were grown in 2% (w/v) solution of malt extract and wheat flour for 4 ...201122134862
efficient glycosynthase mutant derived from mucor hiemalis endo-beta-n-acetylglucosaminidase capable of transferring oligosaccharide from both sugar oxazoline and natural n-glycan.endo-m, an endo-beta-n-acetylglucosaminidase from mucor hiemalis, is a family 85 glycoside hydrolase. this enzyme is unique in that it can transfer en bloc the oligosaccharide of various types of n-glycans onto different acceptors, and thereby it enzymatically generates diverse glycoconjugates. in this study, we performed mutational and kinetic studies focusing on a key catalytic asparagine 175 of endo-m. we have shown that most of the asn-175 mutants had significantly diminished hydrolysis acti ...201019880511
effects of essential oils from eucalyptus globulus leaves on soil organisms involved in leaf degradation.the replacement of native portuguese forests by eucalyptus globulus is often associated with deleterious effects on terrestrial and aquatic communities. several studies have suggested that such a phenomenon is linked with the leaf essential oils released into the environment during the eucalyptus leaf degradation process. however, to date, the way these compounds affect leaf degradation in terrestrial systems i.e. by direct toxic effects to soil invertebrates or indirectly by affecting food of s ...201323577212
a remodeling system for the oligosaccharide chains on glycoproteins with microbial endo-beta-n-acetylglucosaminidases.endo-m, endo-beta-n-acetylglucosaminidase from mucor hiemalis, transferred the complex type oligosaccharide of sialoglycopeptide to partially deglycosylated proteins (n-acetylglucosamine-attached proteins), which were prepared by excluding high-mannose type oligosaccharides from glycoproteins with endo-h, endo-beta-n-acetylglucosaminidase from streptomyces plicatus. this finding indicated that the high-mannose type oligosaccharides on glycoproteins can be changed to complex type ones by the tran ...200617049165
synthesis of neutral glycosphingolipids from zygomycetes.novel neutral glycosphingolipids (ngsls) containing gal-alpha1-->6gal, previously found in the zygomycetes species mucor hiemalis, were synthesized. the structures of these compounds are different from those of other fungal gsls, and they are expected to be involved in host-parasite interactions. a key step in their synthesis is direct 1,2-cis alpha-selective galactosylation of 4,6-diol tri- and tetrasaccharide acceptors with a galactosyl donor in the presence of n-iodosuccinimide (nis)/trifluor ...200818534568
development of a novel pcr assay for the identification of the black yeast, exophiala (wangiella) dermatitidis from adult patients with cystic fibrosis (cf).cystic fibrosis (cf) patients may suffer increased morbidity and mortality through colonisation, allergy and invasive infection from fungi. the black yeast, exophiala dermatitidis (synonym wangiella dermatitidis) has been found with increasing frequency in sputum specimens of cf patients, with reported isolation rates ranging from 1.1 to 15.7%. at present, no diagnostic pcr exists to aid with the clinical laboratory detection and identification of this organism. a novel species-specific pcr-base ...200818571996
the structure and behavior of the nuclei in spores and growing hyphae of mucorales. i. mucor hiemalis and mucor fragilis. 195713460827
chemo-enzymatic synthesis of glycosylated insulin using a glcnac tag.artificial insulin with an n-linked oligosaccharide was synthesized by a chemo-enzymatic method using endo-beta-n-acetylglucosaminidase from mucor hiemalis (endo-m). glcnac-modified insulin was prepared by the reaction of the carboxymethyl glycoside of glcnac and 3 amino groups of bovine insulin using a dimethylphosphinothioic mixed anhydride (mpt-ma) method. a transglycosylation reaction of the glcnac-modified insulin using endo-m gave mono-transglycosylated insulin predominantly. we determined ...201020036558
production of obionin a and derivatives by the sooty blotch fungus microcyclospora malicola.a multitude of sooty blotch and flyspeck fungi, mainly belonging to the ascomycetes order capnodiales, causes dark blemishes and flyspeck-like spots on apples worldwide. different sooty blotch and flyspeck fungi can coexist in the same orchard and even on a single fruit. our preceding experiments revealed an activity of microcyclospora malicola strain 1930 against the anthracnose fungus colletotrichum fioriniae in dual culture assays. extracts of m. malicola strain 1930 showed a broad bioactivit ...201525856439
acceptor specificity in the transglycosylation reaction using determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-n-acetylglucosaminidase (engase) (ec from mucor hiemalis (endo-m), several acceptor derivatives were designed and synthesized. the narrow regions of the 1,3-diol structure from the 4- to 6-hydroxy functions of glcnac were found to be essential for the transglycosylation reaction using endo-m. furthermore, it was determined that endo-m strictly recognizes a 1,3-diol structure ...201020934176
simultaneous detection and identification of aspergillus and mucorales species in tissues collected from patients with fungal rhinosinusitis.rapid detection and differentiation of aspergillus and mucorales species in fungal rhinosinusitis diagnosis are desirable, since the clinical management and prognosis associated with the two taxa are fundamentally different. we describe an assay based on a combination of broad-range pcr amplification and reverse line blot hybridization (pcr/rlb) to detect and differentiate the pathogens causing fungal rhinosinusitis, which include five aspergillus species (a. fumigatus, a. flavus, a. niger, a. t ...201121325541
hyafurones, hyapyrrolines, and hyapyrones: polyketides from hyalangium new polyketides, for which the trivial names hyafurones a1-b (1-3), hyapyrrolines a (4) and b (5), and hyapyrones a (6) and b (7) are proposed, were isolated from the fermentation broth of the myxobacteria hyalangium minutum, strains nocb-2(t) and hym-3. their structures were elucidated from nmr and hresims data, and their geometric configuration was assigned based on noe and vicinal (1)h coupling data. both hyafurone b (3) and hyapyrone b (7) inhibited growth of the gram-positive bacteriu ...201424848583
purification and characterization of an extracellular lipase from mucor hiemalis f. corticola isolated from soil.we have screened 39 microfungi isolates originated from soil in terms of lipolytic activity. out of all screened, a novel strain of mucor hiemalis f. corticola was determined to have the highest lipase activity. the extracellular lipase was produced in response to 2% glucose and 2.1% peptone. the lipase was purified 12.63-folds with a final yield of 27.7% through following purification steps; ammonium sulfate precipitation, dialysis, gel filtration column chromatography and ion exchange chromato ...201222652088
isolation of mucorales from processed maize (zea mays l.) and screening for protease activity.mucorales were isolated from maize flour, corn meal and cooked cornflakes using surface and depth plate methods. rhizopus oryzae, circinella muscae, mucor subtilissimus, mucor hiemalis f. hiemalis, syncephalastrum racemosum, rhizopus microsporus var. chinensis and absidia cylindrospora showed protease activity.200824031292
mucor hiemalis mediated 14α-hydroxylation on steroids: in vivo and in vitro investigations of 14α-hydroxylase activity.transformation of testosterone and progesterone into synthetically challenging 14α-hydroxy derivatives was achieved by using fungal strain mucor hiemalis. prolonged incubation led to the formation of corresponding 6β/7α,14α-dihydroxy metabolites. the position and stereochemistry of newly introduced hydroxyl group was determined by detailed spectroscopic analyses. the time course experiment indicated that fungal strain initiated transformation by hydroxylation at 14α-position followed by at 6β- o ...201424747772
antifungal activity of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides: a novel type of compound afforded by azide-enolate (3+2) cycloaddition.the first report of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides (7a and 7b) is described herein. azide-enolate (3+2) cycloaddition afforded the synthesis of this novel type of compound. antifungal activity was evaluated in vitro against four filamentous fungi (aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae and mucor hiemalis) as well as nine species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 7a and 7b are promising candidates for co ...201627656774
interspecific competitive interactions between pairs of fungal species in natural substrates.the role of interspecific competition in fungal communities in natural substrates is poorly understood because fungi do not form easily definable populations. a new approach to investigating fungal competition, using natural substrates containing a range of known biomass concentrations of each of two species, is described. relative competitive success of each species is assessed over time in terms of propagule production and substrate colonisation by each species. in an agricultural soil mucor h ...199328314028
azide-enolate 1,3-dipolar cycloaddition in the synthesis of novel triazole-based miconazole analogues as promising antifungal miconazole analogs involving 1,4,5-tri and 1,5-disubstituted triazole moieties were synthesized by azide-enolate 1,3-dipolar cycloaddition. the antifungal activity of these compounds was evaluated in vitro against four filamentous fungi, including aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae, and mucor hiemalis as well as three species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 4b, 4d and 7b can be considered as drug candidates fo ...201626890112
antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi.knowledge on the factors that determine the composition of bacterial communities in the vicinity of roots (rhizosphere) is essential to understand plant-soil interactions. plant species identity, plant growth stage and soil properties have been indicated as major determinants of rhizosphere bacterial community composition. here we show that the presence of saprotrophic fungi can be an additional factor steering rhizosphere bacterial community composition and functioning. we studied the impact of ...201526393509
using aquatic fungi for pharmaceutical bioremediation: uptake of acetaminophen by mucor hiemalis does not result in an enzymatic oxidative stress response.the increasing anthropogenic pollution of aquatic environments and fresh water scarcity worldwide have prompted the development of low-cost and effective water treatment alternatives. one example of a highly released anthropogenic xenobiotics is acetaminophen (apap), which has been detected in surface waters at concentrations as high as 5 μg l(-1). to date, traditional water treatment plants were unable to remove all pharmaceutical xenobiotics and as in the case with apap, the breakdown products ...201627647241
transglycosidase-like activity of mucor hiemalis endoglycosidase mutants enabling the synthesis of glycoconjugates using a natural glycan donor.glycan conversion of glycoprotein via the transglycosylation activity of endo-β-n-acetylglucosaminidase is a promising chemoenzymatic technology for the production of glycoproteins including bio-medicines with a homogeneous glycoform. although endo-m is a key enzyme in this process, its product undergoes rehydrolysis, which leads to a lower yield, and limits the practical application of this enzyme. we developed several endo-m mutant enzymes including n175q with glycosynthase-like activity and/o ...201626381157
fungus-associated bacteriome in charge of their host behavior.bacterial-fungal interactions are widespread in nature and there is a growing number of studies reporting distinct fungus-associated bacteria. however, little is known so far about how shifts in the fungus-associated bacteriome will affect the fungal host's lifestyle. in the present study, we describe for the first time the bacterial community associated with the saprotrophic fungus mucor hiemalis, commonly found in soil and rhizosphere. two broad-spectrum antibiotics that strongly altered the b ...201627486066
a facile synthesis of novel miconazole analogues and the evaluation of their antifungal activity.four novel miconazole analogues (8-11) were synthetized and evaluated for activity against four filamentous fungi (mucor hiemalis, aspergillus fumigatus, trichosporon cutaneum, and rhizopus oryzae) and eight species of candida as yeast specimens. compounds 9 and 10 showed very good activity when evaluated in yeast (mic 0.112 and 0.163 μg/ml) compared to the reference compound, itraconazole (mic 0.067 μg/ml). the best antifungal activity in filamentous strains was shown by compound 9. hence compo ...201525989345
complete nmr assignment of a bisecting hybrid-type oligosaccharide transferred by mucor hiemalis endo-β-n-acetylglucosaminidase.this study describes the complete nuclear magnetic resonance (nmr) spectral assignment of a bisecting hybrid-type oligosaccharide 1, transferred by mucor hiemalis endo-β-n-acetylglucosaminidase (endo-m). through (1)h- and (13)c-nmr, dqf-cosy, hsqc, hmbc, tocsy, and noesy experiments, we determine the structure of the glycoside linkage formed by the endo-m transglycosylation, i.e., the connection between glcnac and glcnac in oligosaccharide 1.201627131291
pyrronazols, metabolites from the myxobacteria nannocystis pusilla and n. exedens, are unusual chlorinated pyrone-oxazole-pyrroles.the chlorinated pyrrole-oxazole-pyrones pyrronazol a (1), pyrronazol a2 (2), and pyrronazol b (3) were isolated from nannocystis pusilla strain ari7, and two chlorinated pyrrole-oxazole isomers, pyrronazols c1 (4) and c2 (5), were isolated from n. pusilla strain na a174. hresims, nmr, and x-ray crystallographic analysis was used in the structure elucidation including the absolute configuration of pyrronazol a (1). in addition to pyrronazols, 1,6-phenazine-diol (6) and its glycosyl derivative, 1- ...201424460410
taxonomy and epidemiology of mucor irregularis, agent of chronic cutaneous mucormycosis.mucormycosis usually presents as a progressive infection with significant angio-invasion. mucormycosis due to mucor irregularis (formerly rhizomucor variabilis var. variabilis), however, is exceptional in causing chronic cutaneous infection in immunocompetent humans, ultimately leading to severe morbidity if left untreated. more than 90 % of the cases known to date were reported from asia, mainly from china. the nearest neighbour of m. irregularis is the saprobic species m. hiemalis. the aim of ...201324027346
production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor.the purpose of this study was to evaluate the feasibility of producing bioethanol from palm-oil mill effluent generated by the oil-palm industries through direct bioconversion process. the bioethanol production was carried out through the treatment of compatible mixed cultures such as thrichoderma harzianum, phanerochaete chrysosporium, mucor hiemalis, and yeast, saccharomyces cerevisiae. simultaneous inoculation of t. harzianum and s. cerevisiae was found to be the mixed culture that yielded th ...200919294441
subcutaneous zygomycosis caused by mucor hiemalis in an immunocompetent patient.zygomycosis is an opportunistic fungal infection with a high mortality rate. it is known to cause invasive disease in immunocompromised hosts but it may produce only cutaneous/ subcutaneous infections in immunocompetent hosts. treatment is difficult due to its fulminant course and lack of effective anti-fungal drugs. here, we report a rare case of subcutaneous zygomycosis caused by mucor hiemalis in an immunocompetent patient without any debilitating illness. the patient was successfully treated ...201323940499
fourier transform infrared spectroscopy for the prediction of fatty acid profiles in mucor fungi grown in media with different carbon sources.fungal production of polyunsaturated fatty acids (pufas) is a highly potential approach in biotechnology. currently the main focus is directed towards screening of hundreds strains in order to select of few potential ones. thus, a reliable method for screening a high number of strains within a short period of time is needed. here, we present a novel method for screening of pufa-producing fungi by high-throughput microcultivation and ftir spectroscopy. in the study selected mucor fungi were grown ...201425208488
complexes of potentially pathogenic microscopic fungi in anthropogenic polluted soils.this study was undertaken to investigate the species' diversity and structure of potentially pathogenic microscopic fungal complexes in podzolic soils polluted by fluorine, heavy metals (cu, ni, co), oil products (diesel fuel, gas condensate, mazut). lists of potentially pathogenic fungi isolated from soils are made specifically for north-western part of russia (kola peninsula). the majority of studied fungus species belong to the following genera: penicillium, aspergillus, mucor, lecanicillium ...201323445417
assessment of microbial contamination within working environments of different types of composting plants.the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ...201525947216
[differences in the carbohydrate composition between the yeastlike and mycelial cells of mucor hiemalis]. 201323156687
indiacens a and b: prenyl indoles from the myxobacterium sandaracinus amylolyticus.the gliding bacterium sandaracinus amylolyticus, strain noso-4t, was recently characterized as the first representative of a new myxobacterial genus. a screening of the culture broth for antibiotically active metabolites followed by isolation and characterization revealed two unique 3-formylindol derivatives, indiacen a (1) and its chloro derivative indiacen b (2). both are active against gram-positive and gram-negative bacteria as well as the fungus mucor hiemalis. the biosynthetic origin of th ...201223035772
chemoenzymatic synthesis of hydrophobic glycoprotein: synthesis of saposin c carrying complex-type carbohydrate.the complex-type n-linked octasaccharide oxazoline having lacnac as the nonreducing end sugar was efficiently synthesized using the benzyl-protected lacnac, mannose, and β-mannosyl glcnac units as key building blocks. to achieve a highly β-selective glycosylation with the lacnac unit, the n-trichloroacetyl group was used for the protection of the amino group in the lacnac unit. after complete assembly of these units and deprotection, the obtained free sugar was successfully derivatized into the ...201222800502
mycobiota and mycotoxins in bee pollen collected from different areas of slovakia.contamination by microscopic fungi and mycotoxins in different bee pollen samples, which were stored under three different ways of storing as freezing, drying and uv radiation, was investigated. during spring 2009, 45 samples of bee-collected pollen were gathered from beekeepers who placed their bee colonies on monocultures of sunflower, rape and poppy fields within their flying distance. bee pollen was collected from bees' legs by special devices placed at the entrance to hives. samples were ex ...201121749250
lc-ms/ms method development for quantitative analysis of acetaminophen uptake by the aquatic fungus mucor hiemalis.acetaminophen is a pharmaceutical, frequently found in surface water as a contaminant. bioremediation, in particular, mycoremediation of acetaminophen is a method to remove this compound from waters. owing to the lack of quantitative analytical method for acetaminophen in aquatic organisms, the present study aimed to develop a method for the determination of acetaminophen using lc-ms/ms in the aquatic fungus mucor hiemalis. the method was then applied to evaluate the uptake of acetaminophen by m ...201626950900
cutaneous mucor hiemalis infection diagnosed by molecular methods. 201526341108
rhizosphere and root-infecting fungi and the design of ecological field part of a wider study into the role of soil fungi in the ecology of the winter annual grass, vulpia ciliata ssp. ambigua (le gall) stace & auquier, we applied the fungicides benomyl and prochloraz to three natural populations of the grass growing in east anglia, united kingdom. the rhizosphere and rootinfecting fungi associated with the three populations were analysed each month between february and may 1992 when plants set seed. there were marked differences between the fungal floras associa ...199528306878
generation of a mutant mucor hiemalis endoglycosidase that acts on core-fucosylated n-glycans.endo-β-n-acetylglucosaminidase m (endo-m), an endoglycosidase from the fungus mucor hiemalis, is a useful tool for chemoenzymatic synthesis of glycoconjugates, including glycoprotein-based therapeutics having a precisely defined glycoform, by virtue of its transglycosylation activity. although endo-m has been known to act on various n-glycans, it does not act on core-fucosylated n-glycans, which exist widely in mammalian glycoproteins, thus limiting its application. therefore, we performed site- ...201627629418
production of eicosapentaenoic acid-enriched triacylglycerol by mucor hiemalis ha-30.microorganisms that accumulated the eicosapentaenoic acid (epa)-enriched triacylglycerol (tg), were screened for using yeast-malt medium containing 1% free epa. the best strain was identified as mucor hiemalis ha-30. the optimum culture conditions for the accumulation of epa-enriched tg were : 3% soluble starch, 0.5% polypeptone, 0.3% yeast extract, 0.5% free epa, and ph 6.0 at 25°c. after the cultivation, 1.77 mg/ml of the tg with epa purity of 79% was obtained. the epa content in tg increased ...199927393061
the incorporation and accumulation of supplemented fatty acids in mucor hiemalis ha-30.accumulation of free unsaturated fatty acids, added individually to the medium, into cellular triacylglycerol (tg) were examined using a fungus, mucor hiemalis ha-30, which could produce the eicosapentaenoic acid (20:5n-3)-enriched tg. linoleic (18:2n-6), γ-linolenic (18:3n-6), arachidonic acids (20:4n-6), and 20:5n-3 were favored for accumulation in tg and each fatty acid accumulated in tg at about 80% purity. however, docosahexaenoic acid (22:6n-3) was not efficiently accumulated in tg and its ...199927389336
microbial transformation of quercetin and its prenylated derivatives.the microbial transformation studies of 7-o-prenylquercetin (1), 4'-o-prenylquercetin (2) and quercetin (3) were investigated with 20 different microbial strains to discover new metabolites. it was revealed that the fungus mucor hiemalis was the most appropriate micro-organism which was capable of transforming these flavonoids. structures of the three new (4-6) and one known (7) metabolites were elucidated as 7-o-prenylquercetin 3-o-β-d-glucopyranoside (4), 4'-o-prenylquercetin 3-o-β-d-glucopyra ...201728826260
uptake and biotransformation of pure commercial microcystin-lr versus microcystin-lr from a natural cyanobacterial bloom extract in the aquatic fungus mucor evaluate the remediation efficiency of mucor hiemalis by comparing media elimination, uptake, and biotransformation of microcystin-lr with exposure to pure toxin versus a crude bloom extract.201728597371
ecology, adaptation, and function of methane-sulfidic spring water biofilm microorganisms, including a strain of anaerobic fungus mucor hiemalis.ecological aspects, adaptation, and some functions of a special biofilm and its unique key anaerobic fungus mucor hiemalis strain eh11 isolated from a pristine spring (künzing, bavaria, germany) are described. the spring's pure nature is characterized by, for example, bubbling methane, marine-salinity, mild hydrothermal (~19.1°c), sulfidic, and reductive-anoxic (eh : -241 to -253 mv, o2 : ≤ 0.1 mg/l) conditions. it is geoecologically located at the border zone between bavarian forest (crystallin ...201728544612
chemo-enzymatic synthesis of a glycosylated peptide containing a complex n-glycan based on unprotected oligosaccharides by using dmt-mm and endo-m.for chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (dmt-mm) was used for the synthesis of a n-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. we also performed transglycosylation of an n-glycan to the n-acetylglucosaminyl peptide using endo-β-n-acetylglucosaminidase from mucor hiemalis (endo-m) to synthesize a glycopeptide ...201728523604
responses of the antioxidative and biotransformation enzymes in the aquatic fungus mucor hiemalis exposed to investigate antioxidative and biotransformation enzyme responses in mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications.201728484910
chemo-enzymatic synthesis of the glucagon containing n-linked oligosaccharide and its characterization.the chemo-enzymatic synthesis of an artificially n-glycosylated derivative of glucagon, a peptide hormone that regulates the blood sugar level, is described. we synthesized the glycosylated glucagon by chemical synthesis of an n-acetylglucosaminyl peptide and enzymatic transfer of an oligosaccharide using the transglycosylation activity of the glycosynthase-like mutant of mucor hiemalis endo-β-n-acetylglucosaminidase (endo-m) and sialo-oligosaccharide oxazoline as a donor substrate. the sialo-ol ...201829175660
specificity of donor structures for endo-β-n-acetylglucosaminidase-catalyzed transglycosylation demonstrate the structural specificity of the glycosyl donor for the transglycosylation reaction by using endo-β-n-acetylglucosaminidase from mucor hiemalis (endo-m), a series of tetrasaccharide oxazoline derivatives was synthesized. these derivatives correspond to the core structure of an asparagine-linked glycoprotein glycan with a β-mannose unit of a non-natural-type monosaccharide, including β-glucose, β-galactose, and β-talose in place of the β-mannose moiety. the transglycosylation acti ...201829125207
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