| [presence of antileptospira antibodies in ophidia in argentina]. | sera obtained from 18 bothrops alternatus and 2 phylodrias burmeisteri were analyzed for the presence of antileptospiral antibodies using a microscopic agglutination test. out of 18 bothrops sera, 13 (72%) gave positive results. snake serum reacted with patoc, andamana, wolffi, tarassovi, pomona, pyrogenes and shermani serovars. it can be concluded that leptospiral antibodies are present in snake sera in argentina, suggesting that the circulating antibodies may have an important role in the epiz ... | 1986 | 3685389 |
| ophidian paramyxovirus in brazilian vipers (bothrops alternatus). | | 2006 | 16980527 |
| alternagin-c, a nonrgd-disintegrin, induces neutrophil migration via integrin signaling. | recently, a new protein containing a disintegrin domain, alternagin-c (alt-c), was purified from bothrops alternatus venom. unlike other disintegrins, in alt-c an ecd amino acid mogif takes the place of the rgd sequence. most disintegrins contain an rgd/kgd sequence and are very potent inhibitors of platelet aggregation, as well as other cell interactions with the extracellular matrix, including tumor cell metastasis and angiogenesis. the present study investigated the effects of alt-c on human ... | 2003 | 14653807 |
| kinetics of venom and antivenom serum and clinical parameters and treatment efficacy in bothrops alternatus envenomed dogs. | dogs envenomed with non-lethal doses of bothrops alternatus venom received standard antivenom therapy, im injections of flunixin meglumine, or topical treatmentwith aqueous curcuma longa plant extract. biodistribution of the venom and antivenom were determined by elisa. there was no significant difference in the efficacy of antivenom and plant extract on local effects; flunixin treatment had lower efficacy. distribution of the venom was similar with all 3 treatments. serum levels of the antiveno ... | 2002 | 12458635 |
| [hemorrhagic, edema-forming, proteolytic and myonecrotic activities of viper venoms of bothrops alternatus (vibora de la cruz)]. | a study of hemorrhagic, edema-forming, proteolytic and myonecrotic activities induced by newborn bothrops alternatus snake venoms from argentina was performed. the analyzed venom had these activities with markedly differences when compared with the venom of adult specimens. hemorrhagic activity was four times higher, while edema forming activity was ten times or more. but proteolytic activity was similar in both groups. hystological analysis showed intense hemorrhage and muscular fiber myolisis ... | 1999 | 10797853 |
| [cross neutralization of bothrops jararacussu venom by heterologous antivenoms]. | we have studied the immunochemical cross-reactivity and cross-neutralization of the lethal potency, hemorrhagic, necrotizing, procoagulant and (indirect) hemolytic activities of bothrops jararacussu venom by the standard antivenoms produced in argentina. these antivenoms are horse immunoglobulin f (ab')2 fragments from animals immunized with 1) crotalus durissus terrificus venom (monovalent anticrotalic antivenom); 2) bothrops alternatus and b. neuwiedii venoms (bivalent botropic antivenom); 3) ... | 1999 | 10451561 |
| identification of bothrojaracin-like proteins in snake venoms from bothrops species and lachesis muta. | bothrojaracin, a 27 kda protein isolated from bothrops jararaca venom, forms a non-covalent complex with thrombin, thus blocking its activity. we have previously identified a bothrojaracin-like protein in b. alternatus venom [castro, h.c., dutra, d.l.s., oliveira-carvalho, a.l., zingali, r.b., 1998. bothroalternin, an inhibitor of thrombin from the venom of bothrops alternatus. toxicon 36, 1903-1912]. in this report, we have examined snake venoms from six different bothrops species (b. atrox, b. ... | 1999 | 10414865 |
| a study on the venom yield of venomous snake species from argentina. | a study on the venom yield of snakes from argentina over a three year period was carried out on adult specimens of bothrops alternatus (n = 74); bothrops neuwiedii (n = 127); bothrops ammodytoides (n = 30); bothrops moojeni (n = 14); bothrops jararaca (n = 14); b. jararacussu (n = 6); crotalus durissus terrificus (n = 120) and micrurus spp. (n = 6) as well as with 12 specimens of newborn c. d. terrificus kept in captivity. while for each species there was a positive correlation between venom yie ... | 1998 | 9839679 |
| bothroalternin, a thrombin inhibitor from the venom of bothrops alternatus. | bothroalternin (mw 27 kda), a new member of the family of c-type lectins is a thrombin inhibitor which was purified from pooled b. alternatus venom by affinity chromatography on ppack-thrombin-sepharose, followed by size exclusion and reverse-phase on hplc columns. material retained on the affinity column contained proteins with apparent molecular weights ranging from 20 to 60 kda on sds-page and inhibited aggregation of rabbit platelets induced by alpha-thrombin (ic50 = 28 microg/ml). a single ... | 1998 | 9839674 |
| purification and partial characterization of a thrombin-like enzyme, balterobin, from the venom of bothrops alternatus. | a thrombin-like enzyme, balterobin, was purified from the venom of bothrops alternatus. the purification steps included sephadex g-75, heparin-sepharose and reverse phase hplc c-18 column. balterobin showed an apparent molecular weight of 30,000 in non-reduced conditions and displays a specific coagulant activity of 32.8 nih units/mg over bovine fibrinogen. it also exhibits arginine amidase activity on dl-bapna. like thrombin-like enzymes from other snakes, balterobin possesses valine as n-termi ... | 1998 | 9690798 |
| cross-reactivity and heterologous neutralization of crotaline antivenoms used in argentina. | the immunochemical cross-reactivity and neutralizing capacity of four crotalinae antivenoms consisting in equine f(ab')2 fragments and available in argentina (bothropic bivalent, against bothrops alternatus and b. neuwiedii venoms; bothropic tetravalent, against b. alternatus, b. neuwiedii; b. jararaca and b. jararacussu venoms; bothropic crotalic trivalent, against b. alternatus, b. neuwiedii and crotalus (c.) durissus terrificus venoms and anticrotalic against c. d. terrificus venom) were stud ... | 1998 | 9690795 |
| [hemorrhage induced by snake venoms in argentina]. | mice of 18 and 20 g were injected intradermally with 0.1 ml of serial dilution of venom in saline solution 0.9 buffered ph 7.2. groups of 4 animal were formed, they were sacrificed 2 hours after inoculation. skin of every mouse was put out, and the haemorrhagic area was measured bothrops alternatus, bothrops jararaca, bothrops jararacussu and bothrops neuwiedii venoms were used. crotalus durissus terrificus did not show any haemorrhagic activity. | 1997 | 9580123 |
| inhibition of proteases, myotoxins and phospholipases a2 from bothrops venoms by the heteromeric protein complex of didelphis albiventris opossum serum. | the antibothropic complex (abc) from opossum (species didelphis albiventris) serum was purified by chromatography on deae-sephacel. it showed an acidic character and two polypeptide chains of ca. 45 kda and 48 kda, respectively. lyophilized opossum serum or the abc (100 micrograms), as well as ethylenediamine tetraacetate (0.25 mumoles) were able to completely neutralise the hemorrhagic effect of 50 micrograms of the desiccated venoms of bothrops moojeni, bothrops pirajai and bothrops jararacuss ... | 1997 | 9415818 |
| clinical and epidemiological aspects of the 'urutu' lance-headed viper (bothrops alternatus) bite in a brazilian hospital. | data on 32 cases of bothrops alternatus (the 'urutu' lance-headed viper) bite admitted to a brazilian teaching hospital from 1985 to 1992 were retrospectively surveyed. the majority of the bites occurred within the household area, i.e. in the house or its environs (56.3%), in the 15-49 years age group (74.2%), and in the lower limbs (84.3%). the male to female ratio was 1 to 1. 59.4% of the patients were given the antivenom within two hours of the bite. bites in the household area were commonest ... | 1994 | 7899796 |
| cross-neutralization on the histamine-releasing activity of snake venoms. | this paper investigates the neutralizing effect of crotalic and bothropic antivenoms on the histamine-releasing activity of three different brazilian snake venoms (crotalus durissus terrificus, bothrops jararacussu and bothrops alternatus). this relative histamine-releasing activity was determined on peritoneal mixed cells of rats. c. d. terrificus venom was the most efficient histamine-releasing agent (ed50 = 1.25 micrograms/ml) followed by b. jararacussu (ed50 = 1.97 ug/ml) and b. alternatus ( ... | 1989 | 2485182 |
| a new bradykinin-potentiating peptide (peptide p) isolated from the venom of bothrops jararacussu (jararacuçu tapete, urutu dourado). | several bradykinin potentiators were identified in the venom of bothrops jararacussu by chromatographic techniques and biological assays. one of them which was isolated inhibited the angiotensin-converting enzyme in vitro and potentiated the bradykinin-induced lowering of the arterial pressure in the rat. | 1992 | 1595077 |
| hemostatic effects of recombinant disba-01, a disintegrin from bothrops alternatus. | a monomeric rgd-disintegrin was recently identified from a cdna library from the venom gland of bothrops alternatus. the corresponding 12 kda-recombinant protein, disba-01, specifically interacted with alpha(v)beta3 integrin and displayed potent anti-metastatic and anti-angiogenic properties. here, the interaction of disba-01 with platelet alphaiib beta3 integrin and its effects on hemostasis and thrombosis were investigated. disba-01 bound to chinese hamster ovary (cho) cells expressing beta3 o ... | 2008 | 18508682 |
| a novel alpha(v)beta (3)-blocking disintegrin containing the rgd motive, disba-01, inhibits bfgf-induced angiogenesis and melanoma metastasis. | the integrin alpha(v)beta(3) is involved in multiple aspects of malignant cancer, including tumor angiogenesis and metastasis, which makes the receptor a key target for the development of anti-cancer therapies. we report here on the production, the characterization and the in vivo anti-angiogenic and anti-metastatic properties of a novel alpha(v)beta(3)-binding disintegrin, disba-01, isolated from a cdna library made with rnas from the venom gland of bothrops alternatus. the 11,637 da-recombinan ... | 2008 | 17952617 |
| cytoskeletal rearrangement and cell death induced by bothrops alternatus snake venom in cultured madin-darby canine kidney cells. | bothrops snake venoms cause renal damage, with renal failure being the main cause of death in humans bitten by these snakes. in this work, we investigated the cytoskeletal rearrangement and cytotoxicity caused by bothrops alternatus venom in cultured madin-darby canine kidney (mdck) cells. incubation with venom (10 and 100 microg/ml) significantly (p <0.05) decreased the cellular uptake of neutral red dye after 1 and 3 h. venom (100 microg/ml) also markedly decreased the transepithelial electric ... | 2007 | 17901901 |
| modulation of in vitro and in vivo angiogenesis by alternagin-c, a disintegrin-like protein from bothrops alternatus snake venom and by a peptide derived from its sequence. | we have previously demonstrated that alternagin-c (alt-c), a disintegrin-like protein from the venom of the brazilian snake bothrops alternatus, induces human vascular endothelial cell (huvec) proliferation by up-regulating the expression of vascular endothelial growth factor (vegf). here, we show that alt-c is also able to induce in vivo angiogenesis using the model of matrigel plug in nude mice. fibroblast growth factor (fgf) alone or supplemented with alt-c was mixed with melted matrigel and ... | 2007 | 17428438 |
| structural and functional properties of batx, a new lys49 phospholipase a2 homologue isolated from the venom of the snake bothrops alternatus. | batx pla(2), a k49 phospholipase a(2) homologue was purified from bothrops alternatus venom after two chromatographic steps, molecular exclusion on superdex 75 and reverse phase hplc on mu-bondapack c-18. a molecular mass of 13898.71 da was determined by maldi-tof mass spectrometry. the amino acid composition showed that batx has a high content of lys, tyr, gly, pro, and 14 half-cys residues, typical of a basic pla(2). the complete amino acid sequence of batx pla(2) contains 121 residues, result ... | 2007 | 17270350 |
| alternagin-c, a disintegrin-like protein from the venom of bothrops alternatus, modulates alpha2beta1 integrin-mediated cell adhesion, migration and proliferation. | the alpha2beta1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. alternagin-c (alt-c), a disintegrin-like protein purified from the venom of the brazilian snake bothrops alternatus, competitively interacts with the alpha2beta1 integrin, thereby inhibiting collagen binding. when immobilized in plate wells, alt-c supports the adhesion of fibroblasts as well as of human vein endothelial cells (huvec) and does n ... | 2005 | 16172743 |
| platelet aggregation and antibacterial effects of an l-amino acid oxidase purified from bothrops alternatus snake venom. | the isolation and biochemical/enzymatic characterization of an l-amino acid oxidase, balt-laao-i, from bothrops alternatus snake venom, is described. balt-laao-i is an acidic glycoprotein, pi approximately 5.37, homodimeric, mr approximately 123,000, whose n-terminal sequence is advrnple efretdyevl. it displays a high specificity toward hydrophobic and basic amino acids, while deglycosylation does not alter its enzymatic activity. balt-laao-i induces platelet aggregation and shows bactericidal a ... | 2004 | 15142548 |
| alternagin-c, a disintegrin-like protein, induces vascular endothelial cell growth factor (vegf) expression and endothelial cell proliferation in vitro. | alternagin-c (alt-c), a disintegrin-like protein purified from the venom of the brazilian snake bothrops alternatus, interacts with the major collagen i receptor, the alpha(2)beta(1) integrin, inhibiting collagen binding. here we show that alt-c also inhibits the adhesion of a mouse fibroblast cell line (nih-3t3) to collagen i (ic(50) 2.2 microm). in addition, when immobilized on plate wells, alt-c supports the adhesion of this cell line as well as of human vein endothelial cell (huvec). alt-c ( ... | 2004 | 14766757 |
| venom yield and its relationship with body size and fang separation of pit vipers from argentina. | the amount of venom that a snake can inject is related to its body size. the body size is related to head size and to the distance between fangs. to correlate snake body size, distance between fangs and distance between puncture wounds with the venom yield (and consequently with the venom dose potentially injected in a single snakebite), we studied these variables in two species of public health importance in south america, bothrops (rhinocerophis) alternatus, and crotalus durissus terrificus. i ... | 2016 | 27546705 |
| effects of alternagin-c from bothrops alternatus on gene expression and activity of metalloproteinases in regenerating skeletal muscle. | this study evaluated the effects of alternagin-c (alt-c) on mrna levels of vegf, myod and matrix metalloproteinase 2 (mmp-2) and on activity of mmps in injured tibialis anterior (ta) muscle induced by cryolesioning in rats. thirty-six wistar rats (3 months old, 258.9+/-27 g) were divided into five groups: (1) control group; (2) injured ta and analyzed 3 days later; (3) injured ta treated with alt-c and analyzed 3 days later; (4) injured ta and analyzed 7 days later and (5) injured ta treated wit ... | 2008 | 18761031 |
| systemic alterations induced by a bothrops alternatus hemorrhagic metalloproteinase (baltergin) in mice. | systemic alterations induced by a bothrops alternatus hemorrhagin, named baltergin, a 55kda fibrinogenolytic metalloproteinase isolated from venom of north-eastern argentina specimens, were studied in mice. it caused macroscopic hemorrhagic spots in lungs which was injected intravenously with a minimum pulmonary hemorrhagic dose of 10microg. histological observations of lungs showed mainly hemorrhagic areas, evidenced by the presence of erythrocytes in the alveolar spaces, congestion and increas ... | 2009 | 18983866 |
| interspecific variation in venom composition and toxicity of brazilian snakes from bothrops genus. | the genus bothrops spp. is responsible for 90% of envenomation by snakes in brazil, and the standard treatment for snakebites is the antivenom therapy. the anti-bothropic serum produced by butantan institute is prepared by the hyperimmunization of horses with a pool of venoms from bothrops alternatus, bothrops jararaca, bothrops jararacussu, bothrops moojeni and bothrops neuwiedi. in this study, the biochemical and biological characteristics of the venoms from nineteen snakes of the genus bothro ... | 2008 | 18983867 |
| antigenic, microbicidal and antiparasitic properties of an l-amino acid oxidase isolated from bothrops jararaca snake venom. | venoms from the bee apis mellifera, the caterpillar lonomia achelous, the spiders lycosa sp. and phoneutria nigriventer, the scorpions tityus bahiensis and tityus serrulatus, and the snakes bothrops alternatus, bothrops jararaca, bothrops jararacussu, bothrops moojeni, bothrops neuwiedi, crotalus durissus terrificus, and lachesis muta were assayed (800mug/ml) for activity against staphylococcus aureus. venoms from b. jararaca and b. jararacussu showed the highest s. aureus growth inhibition and ... | 2009 | 19101583 |
| neuromuscular activity of batx, a presynaptic basic pla2 isolated from bothrops alternatus snake venom. | we have previously isolated a lys49 phospholipase a(2) homolog (batx) from bothrops alternatus snake venom using a combination of molecular exclusion chromatography and reverse phase hplc and shown its ability to cause neuromuscular blockade. in this work, we describe a one-step procedure for the purification of this toxin and provide further details of its neuromuscular activity. the toxin was purified by reverse phase hplc and its purity and molecular mass were confirmed by sds-page, maldi-tof ... | 2009 | 19463969 |
| renal kinetics of bothrops alternatus (urutu) snake venom in rats. | the renal kinetics of bothrops alternatus venom (0.8 mg/kg, i.v.) was studied in conscious male wistar rats. blood, urine and renal tissue samples were collected at various intervals after envenoming. venom was quantified by elisa in serum, renal tissue and urine. urine volume was measured and the urine assayed for urobilinogen, glucose, bilirubin, ketones, urine specific gravity, occult blood, ph, protein, nitrite and leucocytes. circulating venom showed biexponential kinetics, with no venom be ... | 2010 | 19818360 |
| a study on the venom of bothrops alternatus duméril, bibron and duméril, from different regions of argentina. | we report a comparison of toxic and enzymatic activities of bothrops alternatus venoms collected from specimens of different regions of argentina with a pool of these same venoms. we determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses, as well as indirect hemolysis, hydrolysis of gelatin and fibrinogen. the electrophoretic pattern of the venoms and that of fibrinogen incubated with each of the venoms was studied by sds-page. the toxic activities were consistent with th ... | 2010 | 20170669 |
| bhalternin: functional and structural characterization of a new thrombin-like enzyme from bothrops alternatus snake venom. | a serine protease from bothrops alternatus snake venom was isolated using deae-sephacel, sephadex g-75 and benzamidine-sepharose column chromatography. the purified enzyme, named bhalternin, ran as a single protein band on analytical polyacrylamide gel electrophoresis (sds-page) and showed molecular weights of 31,500 and 27,000 under reducing and non-reducing conditions, respectively. its complete cdna was obtained by rt-pcr and the 708bp codified for a mature protein of 236 amino acid residues. ... | 2010 | 20184912 |
| snake venoms as a source of compounds modulating sperm physiology: secreted phospholipases a2 from oxyuranus scutellatus scutellatus impact sperm motility, acrosome reaction and in vitro fertilization in mice. | the goal of this study was to identify new compounds from venoms able to modulate sperm physiology and more particularly sperm motility. for this purpose, we screened the effects of 16 snake venoms cleared of molecules higher than 15 kda on sperm motility. venoms rich in neurotoxins like those from oxyuranus scutellatus scutellatus or daboia russelii, were highly potent inhibitors of sperm motility. in contrast, venoms rich in myotoxins like those from echis carinatus, bothrops alternatus and ma ... | 2010 | 20226834 |
| isolation and functional characterization of a new acidic pla(2) ba spii rp4 of the bothrops alternatus snake venom from argentina. | an acidic protein with phospholipase a(2) activity was purified to homogeneity from the venom of the northeast argentinian viperid bothrops alternatus by two chromatographic steps: a conventional gel filtration on sephadex g-75 and reversed phase on c18 hplc column. a molecular mass of 14185.48 da was determined by mass spectrometry, displaying a homodimer conformation. the kinetic assay demonstrated a catalytically active phospholipase a(2) in correspondence with asp49 pla(2) group. the enzyme ... | 2010 | 20331996 |
| rat skin wound healing induced by alternagin-c, a disintegrin-like, cys-rich protein from bothrops alternatus venom. | alternagin-c (alt-c) is a disintegrin-like, cys-rich protein isolated from bothrops alternatus snake venom, which has been shown to induce in vivo angiogenesis. therefore, this protein could be interesting as a new approach for tissue regeneration studies. here the effects of alt-c on fibroblasts and inflammatory cells, collagen type iii and type i and tgf-+¦ expression in a rat wounded skin model were studied. thirty-five male wistar rats (weight 270 -¦ 20 g) were divided into seven groups with ... | 2011 | 21392259 |
| neutralization of bothrops alternatus regional venom pools and individual venoms by antivenom: a systematic comparison. | in this study we report that variation in lethality, hemorrhagic potency and procoagulation between individual samples of bothrops alternatus venom from a single region, and variation between regional pools at the national level are comparable in range. furthermore, the range of relative neutralization potencies of individual venoms within a region overlaps, and sometimes exceeds, the range of neutralization of regional venom pools throughout the country. thus, the potency of neutralization of a ... | 2011 | 21549143 |
| histological and functional renal alterations caused by bothrops alternatus snake venom: expression and activity of na+/k+-atpase. | acute renal failure is a serious complication of human envenoming by bothrops snakes. the ion pump na+/k+-atpase has an important role in renal tubule function, where it modulates sodium reabsorption and homeostasis of the extracellular compartment. here, we investigated the morphological and functional renal alterations and changes in na+/k+-atpase expression and activity in rats injected with bothrops alternatus snake venom. | 2011 | 21704674 |
| histopathological changes caused by venom of urutu snake (bothrops alternatus) in mouse skeletal muscle. | | 1984 | 6533749 |
| lipoxygenase-derived mediators may be involved in in vivo neutrophil migration induced by bothrops erythromelas and bothrops alternatus venoms. | bothrops erythromelas (bev) and b. alternatus (bav) venoms induced a dose-dependent neutrophil migration when injected into rat peritoneal cavities (20-160 micrograms/cavity). these venoms (80 micrograms/rat) also induced neutrophil migration in the air pouch model of inflammation. this migratory response seemed to be related to the phospholipase a2 (pla2) activity of the venoms. bav had approximately two times more pla2 activity than bev, and the neutrophil migration induced by the former venom ... | 1993 | 8146868 |
| geographic variations in the composition of myotoxins from bothrops neuwiedi snake venoms: biochemical characterization and biological activity. | (1) venom pools from bothrops neuwiedi (bn) and from two subspecies, namely bothrops neuwiedi pauloensis (bnp) and bothrops neuwiedi urutu (bnu), collected in the states of são paulo (sp) and minas gerais (mg), brazil, were electrophoretically examined. basic toxins with different isoelectric points were identified in the venom collected in são paulo (bnsp). these toxins were absent in the corresponding pools from minas gerais (bnmg, bnpmg and bnumg). (2) bnsp, but not bnmg, bnpmg or bnumg, show ... | 1998 | 9972319 |
| comparative study of nine bothrops snake venoms from adult female snakes and their offspring. | venoms of seven different bothrops species and three subspecies (b. alternatus, b. cotiara, b. erythromelas, b. jararaca, b. jararacussu, b. moojeni, b. neuwiedi paranaensis. b.n. pauloensis and b.n. urutu) obtained from individual mothers and their young were investigated. biometrics of snakes and protein content, toxicity (ld50), sds-page, proteolytic and clotting activities of venoms were estimated. comparison of venoms from female snakes and their respective newborn offspring were variable i ... | 1991 | 1646500 |
| endogenous phospholipase a2 inhibitors in snakes: a brief overview. | the blood plasma of numerous snake species naturally comprises endogenous phospholipase a2 inhibitors, which primarily neutralize toxic phospholipases a2 that may eventually reach their circulation. this inhibitor type is generally known as snake blood phospholipase a2 inhibitors (sbplis). most, if not all sbplis are oligomeric glycosylated proteins, although the carbohydrate moiety may not be essential for pla2 inhibition in every case. the presently known sbplis belong to one of three structur ... | 2016 | 28031735 |
| inhibition of enzymatic and pharmacological activities of some snake venoms and toxins by mandevilla velutina (apocynaceae) aqueous extract. | phospholipases a(2) (pla(2)) are multifunctional proteins which exhibit varied biological activities correlated to the structural diversities of the sub-classes. the crude aqueous extract from subterranean system of mandevilla velutina, a plant found in brazilian savanna, was assayed for its ability to inhibit biological activities of several snake venoms and isolated pla(2)s. the extract induced total inhibition of the phospholipase activity of crotalus durissus terrificus venom and only partia ... | 2003 | 14644557 |
| the defensive strike of five species of lanceheads of the genus bothrops (viperidae). | we studied the defensive strike of one species of each of five recognized lineages within the genus bothrops, namely, b. alternatus, b. jararaca, b. jararacussu, b. moojeni and b. pauloensis. the defensive strike of the studied species was in general similar to that of crotalus viridis and c. atrox, but some important differences were observed. bothrops alternatus and b. pauloensis struck preferentially from a tight body posture, whereas b. jararaca and b. moojeni from a loose body posture. defe ... | 2007 | 17876444 |
| identification of proteins similar to bothrops jararaca coagulation inhibitor (bji) in the plasmas of bothrops alternatus, bothrops jararacussu and crotalus durissus terrificus snakes. | bothrops jararaca coagulation inhibitor (bji), a protein isolated from b. jararaca plasma, specifically inhibits the coagulant activity of thrombin. our group previously identified proteins similar to bji in the plasma of other snakes [tanaka-azevedo, a.m., tanaka, a.s., sano-martins i.s., 2003. a new blood coagulation inhibitor from the snake bothrops jararaca plasma: isolation and characterization. biochem biophys res commun 308, 706-712.]. in the present study, we analyzed the presence of bji ... | 2008 | 17931922 |
| chromatin supraorganization, dna fragmentation, and cell death in snake erythrocytes. | in nucleate erythrocytes of several vertebrate groups, the frequency and intensity of dna fragmentation associated with programmed cell death vary considerably. although hemoglobin efficiency may be related to erythrocyte life span, and hemoglobin types and erythrocyte life spans are assumed to vary in reptiles, no data on dna fragmentation and chromatin organization as related to cell death exist for snakes. in the present study, chromatin supraorganization, dna fragmentation, and cell death we ... | 2005 | 15746963 |
| humoral immune responses to venom and antivenom of patients bitten by bothrops snakes. | snake envenomation and its treatment cause the entry of two kind of foreign antigens into the human body: snake toxins and antivenom from animal origin. samples of patients bitten by snakes in uruguay were assayed to determine levels of human antibodies against venom and antivenom. the elisa results showed that most of the patients presented an important increase of igg and igm antibodies against antivenom at day 15 post accident. antibodies were reactive against both equine immunoglobulin chain ... | 2011 | 22206812 |
| neutralisation of the pharmacological activities of bothrops alternatus venom by anti-pla2 iggs. | basic phospholipases a2 (pla2) are toxic and induce a wide spectrum of pharmacological effects, although the acidic enzyme types are not lethal or cause low lethality. therefore, it is challenging to elucidate the mechanism of action of acidic phospholipases. this study used the acidic non-toxic ba spii rp4 pla2 from bothrops alternatus as an antigen to develop anti-pla2 igg antibodies in rabbits and used in vivo assays to examine the changes in crude venom when pre-incubated with these antibodi ... | 2014 | 24878372 |
| heterologous expression and biochemical and functional characterization of a recombinant alpha-type myotoxin inhibitor from bothrops alternatus snake. | venomous and non-venomous snakes possess phospholipase a2 (pla2) inhibitory proteins (plis) in their blood serum. this study shows the expression and biochemical and functional characterization of a recombinant alpha inhibitor from bothrops alternatus snake, named rbaltmip. its expression was performed in pichia pastoris heterologous system, resulting in an active recombinant protein. the expressed inhibitor was tested regarding its ability to inhibit the phospholipase activity of different pla2 ... | 2014 | 25047442 |
| genotoxic effect of bothrops snake venoms and isolated toxins on human lymphocyte dna. | in the present study, micronucleus with cytokinesis blocking and comet assays were used to evaluate the genotoxic potential of bothrops jararacussu, bothrops atrox, bothrops moojeni, bothrops alternatus (rhinocerophis alternatus) and bothrops brazili snake venoms, and also of some isolated toxins (mjtx-i, bthtx-i and ii myotoxins, bjussump-ii metalloprotease, and batxlaao l-amino acid oxidase) on human lymphocytes. significant dna damages were observed, indicating genotoxic potential after expos ... | 2013 | 23333649 |
| fluorometric assay using naphthylamide substrates for assessing novel venom peptidase activities. | in the present study we examined the feasibility of using the fluorometry of naphthylamine derivatives for revealing peptidase activities in venoms of the snakes bothrops jararaca, bothrops alternatus, bothrops atrox, bothrops moojeni, bothrops insularis, crotalus durissus terrificus and bitis arietans, of the scorpions tityus serrulatus and tityus bahiensis, and of the spiders phoneutria nigriventer and loxosceles intermedia. neutral aminopeptidase (apn) and prolyl-dipeptidyl aminopeptidase iv ... | 2002 | 12419513 |
| in vitro comparison of enzymatic effects among brazilian bothrops spp. venoms. | in various types of snake venom, the major toxic components are proteinases and members of the phospholipase a2 family, although other enzymes also contribute to the toxicity. in this study, we evaluated the proteolytic, phospholipase, and l-amino acid oxidase activities in the venom of five bothrops species-bothrops jararaca, bothrops jararacussu, bothrops moojeni, bothrops neuwiedi, and bothrops alternatus-all of which are used in the production of commercial antivenom, prepared in horses. the ... | 2013 | 23998940 |
| prospection, structural analysis and phylogenetic relationships of endogenous gamma-phospholipase a(2) inhibitors in brazilian bothrops snakes (viperidae, crotalinae). | during the last 20 years, there have been an increasing number of reports on endogenous phospholipase a(2) inhibitors (plis) in the sera of snakes. these studies have demonstrated the existence of three different structural classes of plis (alpha, beta and gamma). the gamma class members are potent inhibitors of phospholipases a(2) (pla(2)) from the venom of viperidae snakes. these enzymes, together with the mammalian pro-inflammatory pla(2), belong to the iia class of the pla(2)-superfamily. al ... | 2008 | 18620721 |
| phospholipase a2 from bothrops alternatus (víbora de la cruz) venom. purification and some characteristic properties. | one single protein species with phospholipase activity has been isolated from bothrops alternatus venom by a procedure involving gel-filtration on sephadex g-50 (step 1), chromatography on sp-sephadex c-50 (step 2) and gel-filtration on sephadex g-75 (step 3). the purified sample behaved as a homogeneous, monodisperse protein with a molecular weight of 15,000 and isoelectric point of 5.04. the yield in enzyme activity was 48% of the starting material and the apparent purification was 51-fold. wh ... | 1986 | 3715896 |
| mechanism of the cytotoxic effect of l-amino acid oxidase isolated from bothrops alternatus snake venom. | baltlaao-i, an l-amino acid oxidase isolated from bothrops alternatus, is a glycoprotein enzyme with a pi-5.3, 15% sugar and a related molecular mass of 66,000da in its monomeric form, and 123,000da in its dimeric form. the objective of this study is to describe the cytotoxicity activity induced by baltlaao-i isolated from bothrops alternatus venom and its possible mechanism of action on tumor cells. our results clearly depict that baltlaao-i has a strong selective cytotoxic activity on tumor ce ... | 2016 | 27394649 |
| isolation and characterization of a novel metalloprotease inhibitor from bothrops alternatus snake serum. | resistance of snakes and some other animals to snake envenomation has been attributed to soluble factors present in their tissues. here we report the isolation of a novel metalloprotease inhibitor from bothrops alternatus snake serum (named baltmpi) with high purity, using a four-step chromatographic method. baltmpi has molecular weights of 60.5 and 42.4kda, as determined by sds-page and mass spectrometry, respectively, and pi=5.27. the first 60 amino acids from the n-terminal region of baltmpi, ... | 2017 | 28163123 |
| rbaltmip, a recombinant alpha-type myotoxin inhibitor from bothrops alternatus (rhinocerophis alternatus) snake, as a potential candidate to complement the antivenom therapy. | phospholipase a2 inhibitors (plis) are important targets in the search and development of new drugs. this study aimed at evaluating the potential of an alpha-type phospholipase a2 inhibitor from bothrops alternatus (rhinocerophis alternatus) snake in its recombinant form (rbaltmip) to complement the conventional antivenom therapy. biochemical experiments showed that rbaltmip presented pi 5.8 and molecular masses of ∼21 kda by sds-page and 19.57 kda by maldi/tof ms. after tryptic peptides sequenc ... | 2016 | 28327300 |
| molecular characterization of metalloproteases from bothrops alternatus snake venom. | we have previously demonstrated that alternagin-c (alt-c), a disintegrin-like, cys-rich protein isolated from bothrops alternatus snake venom, induces human vascular endothelial cell (huvec) proliferation and angiogenesis in in vitro and in vivo assays. therefore this protein could be interesting as a new approach for tissue regeneration studies. however, its primary sequence was not completely determined since the protein isolated from crude venom is usually a mixture of isoforms. here we descr ... | 2014 | 25463060 |
| [effects of bothrops alternatus venom of argentina on muscle and different organs in mices]. | bothrops alternatus venom was inoculated in gastrocnemius muscle of mice between 18 and 20g. the dose was 50 micrograms in 0.1 ml in clna 0.85% solution. groups of 5 animals were sacrified between 3, 6 and 12 hours after inoculation. hystopathology was performed with muscle and different organs fixed with bouin. the venom reproduced a local inflammatory reaction. histopathology observations of muscle revealed myolytic and coagulative necrosis. there and six hours after inoculation, hepatocellula ... | 1996 | 8998374 |
| rapid purification of serine proteinases from bothrops alternatus and bothrops moojeni venoms. | envenomation by bothrops species results, among other symptoms, in hemostatic disturbances. these changes can be ascribed to the presence of enzymes, primarily serine proteinases some of which are structurally similar to thrombin and specifically cleave fibrinogen releasing fibrinopeptides. a rapid, three-step, chromatographic procedure was developed to routinely purify serine proteinases from the venoms of bothrops alternatus and bothrops moojeni. the serine proteinase from b. alternatus displa ... | 2013 | 24140922 |
| effect of chemical modification with p-bromophenacyl bromide on the enzymatic and lethal properties of phospholipase a2 from bothrops alternatus (víbora de la cruz) venom. | the effects on lethal potency and enzymatic activity were determined following alkylation, with p-bromophenacyl bromide, of the acidic toxic phospholipase a2 from bothrops alternatus. the modified b. alternatus enzyme, which lost its enzymatic activity, retained considerable toxicity. histopathologic studies on mice have demonstrated features similar to those of the native enzyme. however, the distribution of the damage was different and the survival time was longer. it is concluded that the enz ... | 1988 | 3238699 |
| cardiovascular responses to bothrops alternatus (urutu) snake venom in anesthetized dogs. | the cardiovascular responses to bothrops alternatus snake venom in anesthetized dogs were investigated. venom (0.3 mg/kg, i.v.) markedly decreased arterial blood pressure, coronary perfusion pressure, and cardiac output (co) after 5 min, with progressive recovery of the first two parameters to pre-venom levels after 3 h; co showed little recovery. there was an abrupt, sustained decrease in left and right ventricular systolic work and stroke volume but no significant changes in heart rate, electr ... | 2012 | 22528815 |
| contribution of phospholipase a2 to the lethal potency of bothrops alternatus (víbora de la cruz) venom. | purified phospholipase a2 from bothrops alternatus venom is one single protein species with a molecular weight of 15,000 and isoelectric point 5.08. when injected i.p. or i.v. at a dose of 0.7 microgram/g body weight it is lethal to mice, eliciting a typical syndrome of dyspnea, tachycardia, arrhythmia and irreversible shock. post mortem and histopathologic studies have demonstrated that the lungs (massive pulmonary hemorrhage), heart (foci of myocardial and endocardial necrosis with interfibril ... | 1986 | 3775796 |
| blocking αvβ3 integrin by a recombinant rgd disintegrin impairs vegf signaling in endothelial cells. | vascular endothelial growth factor (vegf) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. however, the mechanism of action of such inhibitors is not completely understood. we have previously demonstrated the anti-angiogenic and anti-metastatic properties of disba-01, a recombinant his-tag rgd-disintegrin from bothrops alternatus snake venom ... | 2012 | 22561350 |
| molecular cloning and biochemical characterization of a myotoxin inhibitor from bothrops alternatus snake plasma. | phospholipases a(2) (pla(2)s) are important components of bothrops snake venoms, that can induce several effects on envenomations such as myotoxicity, inhibition or induction of platelet aggregation and edema. it is known that venomous and non-venomous snakes present pla(2) inhibitory proteins (plis) in their blood plasma. an inhibitory protein that neutralizes the enzymatic and toxic activities of several pla(2)s from bothrops venoms was isolated from bothrops alternatus snake plasma by affinit ... | 2011 | 21144879 |
| a transcriptomic analysis of gene expression in the venom gland of the snake bothrops alternatus (urutu). | the genus bothrops is widespread throughout central and south america and is the principal cause of snakebite in these regions. transcriptomic and proteomic studies have examined the venom composition of several species in this genus, but many others remain to be studied. in this work, we used a transcriptomic approach to examine the venom gland genes of bothrops alternatus, a clinically important species found in southeastern and southern brazil, uruguay, northern argentina and eastern paraguay ... | 2010 | 20977763 |
| venoms and isolated toxins from snakes of medical impact in the northeast argentina: state of the art. potential pharmacological applications. | among the ophidians that inhabit the northeast of argentina, the genus bothrops such as b. alternatus and b. diporus species (also known as yararás) and crotalus durisus terrificus (named cascabel), represent the most studied snake venom for more than thirty years. these two genera of venomous snakes account for the majority of poisonous snake envenomations and therefore, constitute a medical emergency in this region. this review presents a broad description of the compiled knowledge about venom ... | 2019 | 31345151 |
| isolation, structural and functional characterization of a new lys49 phospholipase a2 homologue from bothrops neuwiedi urutu with bactericidal potential. | snake venom is a complex mixture of active compounds consisting of 80-90% proteins and peptides that exhibit a variety of biological actions that are not completely clarified or identified. of these, phospholipase a2 is one of the molecules that has shown great biotechnological potential. the objectives of this study were to isolate, biochemically and biologically characterize a lys49 phospholipase a2 homologue from the venom of bothrops neuwiedi urutu. the protein was purified after two chromat ... | 2016 | 26927324 |
| in vitro tracking of phospholipase a2 from snake venom conjugated with magic-sized quantum dots. | phospholipases a2 represent a family of enzymes with important application in medicine. however, direct tracking is difficult due to the absence of a stable, effective and specific marker for these enzymes. magic-sized quantum dots (msqds) are inorganic semiconducting nanocrystals with unique physical properties. they have the ability to conjugate to proteins, making them excellent markers for biological systems. in this work, we labelled phospholipase a2 from bothrops alternatus snake venom wit ... | 2019 | 30385337 |
| purification and characterization of a phosphodiesterase from bothrops alternatus snake venom. | a phosphodiesterase was purified from the venom of the snake bothrops alternatus by a combination of gel filtration and ion exchange chromatographies. in sds-page, the enzyme gave a single band with a molecular mass of 105 kda, which was unaltered in the presence of beta-mercaptoethanol, indicating that the protein contained no subunits. a single protein band was also observed in native page. there were no contaminating 5'-nucleotidase, alkaline phosphatase and protease activities. the enzyme wa ... | 2002 | 12638651 |
| the bov-b lines found in vipera ammodytes toxic pla2 genes are widespread in snake genomes. | in the fourth intron of two toxic vipera ammodytes pla2 genes a ruminantia specific 5'-truncated bov-b line element was identified. southern blot analysis of bov-b line distribution in vertebrates shows that, apart from the ruminantia, it is limited to viperidae snakes (v. ammodytes, vipera palaestinae, echis coloratus, bothrops alternatus, trimeresurus flavoviridis and trimeresurus gramineus). the copy number of the 3' end of bov-b line in the v. ammodytes genome is between 62,000 and 75,000. a ... | 1998 | 9792174 |
| effect of monospecific antibodies against baltergin in myotoxicity induced by bothrops alternatus venom from northeast of argentina. role of metalloproteinases in muscle damage. | myotoxicity, one of the most relevant local manifestations in envenomation by bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. baltergin, a snake venom metalloproteinase (svmp), isolated from bothrops alternatus venom has been used to obtain monospecific igg, in order to determine the relative role of toxin in myotoxicity induced by whole venom. bothrops diporus venom, another medical relevant genus of the northeastern regi ... | 2013 | 23246580 |
| purification of a fragment obtained by autolysis of a piiib-svmp from bothrops alternatus venom. | | 2018 | 29471097 |
| evolution of venom antigenaemia and antivenom concentration in patients bitten by snakes in uruguay. | in this work we describe the first study carried out in uruguay of venom antigenaemia and antivenom concentration in patients bitten by snakes. between 50 and 70 snake bite accidents per year are caused in uruguay by 2 species: rhinocerophis alternatus and bothropoides pubescens. the patients are treated with a specific polyvalent antivenom. gaining insight on the evolution of venom antigenaemia and antivenom concentration in patients is important to improve treatment protocols. blood samples of ... | 2012 | 22819686 |
| resource partitioning in a snake assemblage from east-central argentina. | two dimensions of the ecological niche (diet and habitat) of a snake assemblage from an endemic rich area in east-central argentina, the sierras de ventania mountain chain, were analyzed. field data collection was performed in 15-week study periods between 2010 and 2014. snakes were hand-captured using transect surveys. field observations on diet were analyzed together with stomach content data from museum specimens. our results supported the partitioning of the snake assemblage by both habitat ... | 2020 | 32520217 |
| bov-b long interspersed repeated dna (line) sequences are present in vipera ammodytes phospholipase a2 genes and in genomes of viperidae snakes. | ammodytin l is a myotoxic ser49 phospholipase a2 (pla2) homologue, which is tissue-specifically expressed in the venom glands of vipera ammodytes. the complete dna sequence of the gene and its 5' and 3' flanking regions has been determined. the gene consists of five exons separated by four introns. comparative analysis of the ammodytin l and ammodytoxin c genes shows that all intron and flanking sequences are considerably more conserved (93-97%) than the mature protein-coding exons. the pattern ... | 1997 | 9219538 |
| alternagin-c (alt-c), a disintegrin-like cys-rich protein isolated from the venom of the snake rhinocerophis alternatus, stimulates angiogenesis and antioxidant defenses in the liver of freshwater fish, hoplias malabaricus. | alternagin-c (alt-c) is a disintegrin-like protein isolated from rhinocerophis alternatus snake venom, which induces endothelial cell proliferation and angiogenesis. the aim of this study was to evaluate the systemic effects of a single dose of alternagin-c (0.5 mg·kg-1, via intra-arterial) on oxidative stress biomarkers, histological alterations, vascular endothelial growth factor (vegf) production, and the degree of vascularization in the liver of the freshwater fish traíra, hoplias malabaricu ... | 2017 | 28956818 |
| the disintegrin-like domain of the snake venom metalloprotease alternagin inhibits alpha2beta1 integrin-mediated cell adhesion. | the alpha2beta1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. here we describe the isolation of a novel metalloproteinase/disintegrin, which is a potent inhibitor of the collagen binding to alpha2beta1 integrin. this 55-kda protein (alternagin) and its disintegrin domain (alternagin-c) were isolated from bothrops alternatus snake venom. amino acid sequencing of alternagin-c revealed the disintegrin struct ... | 2000 | 11368322 |
| arsenic and other trace elements in two catfish species from paranaguá estuarine complex, paraná, brazil. | concentrations of arsenic and four additional trace elements (cu, cr, ni, and zn) were determined by inductively coupled plasma-optical emission spectrometry in the muscular tissue of the yellow catfish (cathorops spixii) and the urutu catfish (genidens genidens) from paranaguá estuarine complex, brazil (pec). the pec can be characterized by an environment of high ecological and economic importance in which preserved areas of rainforest and mangroves coexist with urban activities as ports and in ... | 2013 | 23584825 |
| bag, a new dimeric metalloproteinase/disintegrin from the bothrops alternatus snake venom that interacts with alpha5beta1 integrin. | the alpha(5)beta(1) integrin is one of the major fibronectin receptors which plays an essential role in the adhesion of normal and tumor cells to extracellular matrix. here, we describe the isolation and characterization of a novel dimeric metalloproteinase/disintegrin, which is an inhibitor of fibronectin binding to the alpha(5)beta(1) integrin. this protein (bag) was isolated from the venom of the south american snake bothrops alternatus by gelatin-sepharose affinity and anion exchange chromat ... | 2003 | 12893294 |
| neutralization of pharmacological and toxic activities of bothrops snake venoms by schizolobium parahyba (fabaceae) aqueous extract and its fractions. | the aqueous extract prepared from schizolobium parahyba (sp) leaves, a native plant from atlantic forest (brazil), was tested to analyse its ability to inhibit some biological and enzymatic activities induced by bothrops alternatus (baltcv) and bothrops moojeni (bmoocv) snake venoms. sp inhibited 100% of lethality, blood incoagulability, haemorrhagic and indirect haemolytic activities at a 1:10 ratio (venom/extract, w/w), as well as coagulant activity at a 1:5 ratio (venom/extract, w/w) induced ... | 2008 | 18598303 |
| recombinant rgd-disintegrin disba-01 blocks integrin αvβ3 and impairs vegf signaling in endothelial cells. | integrins mediate cell adhesion, migration, and survival by connecting the intracellular machinery with the surrounding extracellular matrix. previous studies demonstrated the interaction between αvβ3 integrin and vegf type 2 receptor (vegfr2) in vegf-induced angiogenesis. disba-01, a recombinant his-tag fusion, rgd-disintegrin from bothrops alternatus snake venom, binds to αvβ3 integrin with nanomolar affinity blocking cell adhesion to the extracellular matrix. here we present in vitro evidence ... | 2019 | 30894182 |
| species distribution models and empirical test: comparing predictions with well-understood geographical distribution of bothrops alternatus in argentina. | species distribution models (sdms) estimate the geographical distribution of species although with several limitations due to sources of inaccuracy and biases. empirical tests arose as the most important steps in scientific knowledge to assess the efficiency of model predictions, which are poorly rigorous in sdms. a good approach to the empirical distribution (ed) of a species can be obtained from comprehensive empirical knowledge, that is, well-understood distributions gathered from large amoun ... | 2018 | 30464822 |
| genomic features of a multidrug-resistant enterobacter cloacae st279 producing ctx-m-15 and aac(6')-ib-cr isolated from fatal infectious stomatitis in a crossed pit viper (bothrops alternatus). | the widespread dissemination of extended-spectrum β-lactamase-producing enterobacteriaceae has become a major issue in veterinary medicine. however, until now, there has been no report of bacteria with such a phenotype in infected snakes. the aim of this study was to report the first draft genome sequence of an enterobacter cloacae isolate (serp1) recovered from a snake with infectious stomatitis. | 2018 | 30448520 |
| cardioprotective effects of alternagin-c (alt-c), a disintegrin-like protein from rhinocerophis alternatus snake venom, on hypoxia-reoxygenation-induced injury in fish. | alternagin-c (alt-c) is a disintegrin-like peptide purified from rhinocerophis alternatus snake venom with the property of inducing vascular endothelial growth factor (vegf) expression, endothelial cell proliferation and migration, and angiogenesis. therefore, this protein could be interesting as a new approach for ischemic heart diseases, an imbalance between myocardial oxygen supply and demand, leading to cardiac dysfunction. we investigated the effects of a single dose of alternagin-c (0.5 mg ... | 2019 | 30352307 |
| baltpla2: a new phospholipase a2 from bothrops alternatus snake venom with antiplatelet aggregation activity. | in last decades, snake venoms have aroused great interest of the medicine due to the pathophysiological effects caused by their toxins. these include the phospholipases a2, low molecular weight proteins capable of causing haemorrhagic, myotoxic, inflammatory and neurotoxic effects after an ophidian accident. the present work describes the isolation and biochemical characterization of a new pla2 isolated from the b. alternatus snake venom, which was named baltpla2. | 2018 | 30289061 |
| alternagin-c binding to α2β1 integrin controls matrix metalloprotease-9 and matrix metalloprotease-2 in breast tumor cells and endothelial cells. | matrix metalloproteinases (mmps) are key players in tumor progression, helping tumor cells to modify their microenvironment, which allows cell migration to secondary sites. the role of integrins, adhesion receptors that connect cells to the extracellular matrix, in mmp expression and activity has been previously suggested. however, the mechanisms by which integrins control mmp expression are not completely understood. particularly, the role of α2β1 integrin, one of the major collagen i receptors ... | 2018 | 29713337 |
| evaluation of pro-inflammatory events induced by bothrops alternatus snake venom. | inflammation is a major local feature of envenomation by bothropic snakes being characterized by a prominent local edema, pain, and extensive swelling. there are reports demonstrating that whole bothrops snake venoms and toxins isolated from them are able to activate macrophages functions, such as phagocytosis, production of reactive oxygen, cytokines and eicosanoids, however, little is known about the effects of bothrops alternatus (b.a.) venom on macrophages. in this work, we evaluated the pro ... | 2018 | 29248447 |
| baltdc: purification, characterization and infrared spectroscopy of an antiplatelet dc protein isolated from bothrops alternatus snake venom. | snake venoms are a complex mixture of proteins, organic and inorganic compounds. some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. the possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. the present study aimed to purify and characterize an antiplatelet dc protein from bothrops alternatus snake venom. | 2017 | 28775739 |
| apoptosis induced by a snake venom metalloproteinase from bothrops alternatus venom in c2c12 muscle cells. | in this study, the apoptosis inducing effects of baltergin as well as its influence on cell adhesion and migration on muscles cells in vitro were studied. morphological analysis made by scanning electron and phase contrast microscopy demonstrated typical futures of programmed cell death, apoptosis. this mechanism was confirmed by fluorescence staining, molecular analysis of endonuclease activity and increased mrna expression level of two representative genes (p53 and bax). on the other hand, bal ... | 2017 | 28205127 |
| catechin and epicatechin as an adjuvant in the therapy of hemostasis disorders induced by snake venoms. | snake toxins, such as phospholipases a2 and proteases, are used as research tools to evaluate biological activities and to understand physiopathological processes of natural compounds better. in the present study, the phenolic compounds catechin and epicatechin were incubated with snake venoms to evaluate their inhibition against different substrates. catechin and epicatechin exerted inhibitions between 20% and 95% on the activity of phospholipases a2 present in the venom of bothrops alternatus. ... | 2020 | 32830399 |
| efficient muscle regeneration after highly haemorrhagic bothrops alternatus venom injection. | bothrops alternatus snake venom is particularly characterized for inducing a prominent haemorrhage and affecting hemostasis as a consequence of 43.1% of metallo-proteinases and less than 10% of pla2 (almost all non-myotoxic phospholipases) in its venomics. in addition, myonecrosis is the major local effect in viper envenoming which might lead to permanent sequela. then, the rebuilding of the microvasculature at the local injured site acquires significance since represents one of the pivotal stag ... | 2016 | 27720976 |
| insights into the substrate specificity of a novel snake venom serine peptidase by molecular modeling. | the cdna encoding bthatl, a serine peptidase from the venom of the snake bothrops alternatus, was cloned and sequenced. the deduced primary structure shows over 62% of identity with snake venom thrombin-like enzymes (svtles), molecules with high substrate specificity toward different natural substrates. indeed, a phylogenetic reconstruction by two different methods clustered this enzyme close to other svtles. these enzymes generally affect the hemostatic system in several ways, and therefore are ... | 2006 | 16716626 |
| molecular interactions between p-coumaric acid and snake venom toxins. | a large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. the interactions between the phenolic compound p-coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. the p-coumaric acid was able to inhibit 31% of the phospholipase activity induced by bothrops alternatus venom, 27% of the hemolytic activity induced by b. moojeni, 62.5% of the thrombolytic activ ... | 2019 | 31016790 |
| inhibitory effects of ascorbic acid, vitamin e, and vitamin b-complex on the biological activities induced by bothrops venom. | natural compounds have been widely studied with the aim of complementing antiophidic serum therapy. | 2016 | 26452622 |
| the modulation of striatonigral and nigrotectal pathways by cb1 signalling in the substantia nigra pars reticulata regulates panic elicited in mice by urutu-cruzeiro lancehead pit vipers. | cannabinoid receptor type 1 (cb1r) is widely distributed in the substantia nigra pars reticulata (snpr). however, the role of cb1r at the snpr level in threatening situations is poorly understood. we investigated the role of cb1r in the snpr on the expression of fear responses in mice confronted with urutu-cruzeiro pit vipers. first, a bidirectional neurotracer was injected into the snpr; then, immunostaining of the vesicular gaba transporter was conducted at the levels of the striatum (cpu) and ... | 2020 | 33171147 |
| alternagin-c (alt-c), a disintegrin-like protein, attenuates alpha2beta1 integrin and vegf receptor 2 signaling resulting in angiogenesis inhibition. | angiogenesis, a crucial process in tumor progression, is mainly regulated by vascular endothelial growth factor (vegf) and its receptor, vegfr2. studies have shown the interaction between α2β1 integrin, a collagen receptor, and vegfr2 in vegf-driven angiogenesis in vitro and in vivo. alternagin-c (alt-c), an ecd-disintegrin-like protein from bothrops alternatus snake venom, has high affinity for α2β1 integrin and shows antiangiogenic activity in concentrations higher than 100 nm. despite previou ... | 2020 | 32360415 |
| alternagin-c, a disintegrin-like protein from bothrops alternatus venom, attenuates inflammation and angiogenesis and stimulates collagen deposition of sponge-induced fibrovascular tissue in mice. | alternagin-c (alt-c), a disintegrin-like protein obtained from the venom of bothrops alternatus, is able to modulate cellular behaviors such as adhesion, migration and proliferation, as well as the production of various growth factors via α2β1 integrin, important processes during inflammation, angiogenesis and fibrogenesis, which although appear as distinct events, act concomitantly in several chronic inflammatory diseases. our objective was to investigate the effects of alt-c on components of t ... | 2019 | 31442506 |
| first report on baltcrp, a cysteine-rich secretory protein (crisp) from bothrops alternatus venom: effects on potassium channels and inflammatory processes. | crisps represent a family of cysteine-rich secretory proteins with molecular mass between 20 and 30 kda and a highly conserved specific pattern of 16 cysteine residues. in this work, we isolated and characterized a novel crisp from bothrops alternatus venom, named baltcrp, also evaluating its effects on different isoforms of potassium channels (kv1.1; kv1.2; kv1.3; kv1.4; kv1.5; kv2.1; kv10.1 and shaker) and on inflammatory processes in vivo. this toxin has a molecular mass of 24.4 kda and pi ar ... | 2019 | 31419561 |