Publications

l-696,474, a novel cytochalasin as an inhibitor of hiv-1 protease. i. the producing organism and its fermentation.	a novel cytochalasin, l-696,474, (18-dehydroxy cytochalasin h) that inhibits hiv-1 protease was discovered in fermentations of a bark-inhabiting ascomycete, hypoxylon fragiforme. the product was first identified from extracts of an agar medium. fermentation studies on a number of media indicated that the product can be made on several solid and liquid media. optimum production was obtained from growth in a complex medium composed of glycerol, glucose, citrate, ardamine, soybean meal, tomato past ...	1992	1624369
l-696,474, a novel cytochalasin as an inhibitor of hiv-1 protease. ii. isolation and structure.	a novel hiv-1 protease inhibitor, l-696,474 (c30h39no4, 477), was isolated from the fermentations of the fungus hypoxylon fragiforme (atcc 20995, mf5511) and purified by silica gel chromatography followed by crystallization. spectroscopic studies have shown the competitive inhibitor l-696,474 to be a novel cytochalasin. two related novel cytochalasins were also isolated and had no effect on the enzyme.	1992	1624370
l-696,474, a novel cytochalasin as an inhibitor of hiv-1 protease. iii. biological activity.	l-696,474, an inhibitor of the hiv-1 protease, was discovered in extracts of the fungal culture hypoxylon fragiforme (mf5511; atcc 20995). l-696,474 is a novel cytochalasin with a molecular weight of 477 and an empirical formula of c30h39no4. l-696,474 inhibited hiv-1 protease activity with an ic50 of 3 microm and the mode of inhibition was competitive with respect to substrate (apparent ki = 1 microm). furthermore, l-696,474 was not a slow-binding inhibitor. the inhibition due to l-696,474 was ...	1992	1624371
changes in secondary metabolism during stromatal ontogeny of hypoxylon fragiforme.	stromata of hypoxylon fragiforme were studied during the vegetation period by hplc profiling, revealing changes in the composition during stromatal development. cytochalasin h and two new cytochalasins named fragiformins a-b were identified as major constituents of the young, maturing stromata, whereas mature, ascogenous material yielded large amounts of mitorubrin-type azaphilones. the above compounds, further cytochalasins from xylariaceae and other fungi, and additional azaphilones of the mit ...	2006	16876700
co-cultured production of lignin-modifying enzymes with white-rot fungi.	co-cultivation was a potential strategy in lignocellulolytic biodegradation with producing high activity enzymes due to their synergistic action. the objective of this study was to investigate the rarely understood effects of co-culturing of two white-rot fungi on lignin-modifying enzymes (lmes) production. six species, bjerkandera adusta, phlebia radiata, pleurotus ostreatus, dichomitus squalens, hypoxylon fragiforme and pleurotus eryngii, were cultured in pairs to study the production of lmes. ...	2011	21647688