Publications

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development of a multiplex pcr for identification of vineyard mealybugs.a simple molecular tool was developed and tested to identify seven mealybug species found in north american vineyards: pseudococcus maritimus ehrhorn, pseudococcus viburni (signoret), pseudococcus longispinus (targioni-tozzeti), pseudococcus calceolariae (maskell), planococcus ficus (signoret), planococcus citri (risso), and ferrisia gilli gullan. the developed multiplex pcr is based on the mitochondrial cytochrome c oxidase subunit one gene. in tests, this single-step multiplex pcr correctly i ...201122217778
Molecular and histological characterisation of primary (ß-proteobacteria) and secondary (?-proteobacteria) endosymbionts of three species of mealybugs (Pseudococcus longispinus, Pseudococcus calceolariae, Pseudococcus viburni) (Pseudococcidae, Hemiptera) and prediction of interactions of their GroEL homologs with Grape Leaf Roll-associated Virus-3.Microscopic localization of endosymbiotic bacteria in three species of mealybug (Pseudococcus longispinus, long-tailed mealybug; Pseudococcus calceolariae, citrophilus mealybug; and Pseudococcus. viburni, obscure mealybug) showed these organisms were confined to bacteriocyte cells within a bacteriome centrally located within the haemocoel. Two species of bacteria were present, with the secondary endosymbiont, in all cases, living within the primary endosymbiont. DNA from the dissected bacteriome ...201122156418
scale insects (hemiptera: coccomorpha) on sugarcane in colombia, with description of a new species of tillancoccus ben-dov (coccidae).herein we describe a new species, tillancoccus koreguajae caballero & ramos, sp. n. (hemiptera: coccidae) from colombia collected on sugarcane. pinnaspis strachani (cooley) is also recorded on sugarcane for the first time worldwide. an updated list of seven species of coccomorpha on sugarcane in colombia is provided, including information on its distribution, biology, and mutualistic ants for each species. seven species of scale insects (hemiptera: coccomorpha) have been recorded previously on s ...201728609908
spatial analysis of mass trapping: how close is close enough?the identification of new attractants can present opportunities for developing mass trapping, but standard screening methods are needed to expedite this. we have developed a simple approach based on quantifying trap interference in 4 × 4 trap arrays with different spacings. we discuss results from sex pheromones in lepidoptera (light brown apple moth, epiphyas postvittana), diptera (apple leaf curling midge, dasineura mali) and homoptera (citrophilous mealybug, pseudococcus calceolariae), compar ...201525470004
the absolute configuration of the sex pheromone of the citrophilous mealybug, pseudococcus calceolariae.the absolute configuration of the sex pheromone of the citrophilous mealybug, pseudococcus calceolariae, was determined to be (1r,3r)-[2,2-dimethyl-3-(2-methylprop-1-enyl)cyclopropyl]methyl (r)-2-acetoxy-3-methylbutanoate. nmr, derivatization reactions, chiral gas chromatography-mass spectrometry, and comparison with synthetic chiral reference compounds, were used to determine the absolute configuration of this compound. this activity of this compound was further confirmed by testing synthetic s ...201121243408
relationship of dna methylation level to the presence of heterochromatin in mealybugs.purified nuclear dna from two mealybug species was analyzed for its 5-methylcytosine (m5c) content by reversed-phase high-pressure liquid chromatography. we observed that the percent m5c (percentage of cytosines which are methylated) varied between the two species, between males and females of the same species, and between lines with and without supernumerary b chromosomes. this is the first case of a sex-specific difference in overall dna methylation level. in contrast to a recent report (deoba ...19846717436
monitoring pseudococcus calceolariae (hemiptera: pseudococcidae) in fruit crops using pheromone-baited traps.the citrophilus mealybug, pseudococcus calceolariae (maskell), is an important pest of fruit crops in many regions of the world. recently, its sex pheromone has been identified and synthesized. we carried out field experiments with the goal of developing monitoring protocols for p. calceolariae using pheromone-baited traps. traps checked hourly for 24 hours showed a distinct diel pattern of male flight, between 18:00 and 21:00 h. the presence of unnatural stereoisomers did not affect trap captur ...201526453728
natural enemies of three mealybug species (hemiptera: pseudococcidae) found on citrus and effects of some insecticides on the mealybug parasitoid coccidoxenoides peregrinus (hymenoptera: encyrtidae) in south africa.the population density of mealybug species in some south african citrus orchards has increased to pest status in recent years. the characterization of the natural enemy complex and quantification of their contribution to the control of planococcus citri (risso), pseudococcus longispinus (targioni-tozzetti) and pseudococcus calceolariae (maskell) on citrus limon (l.) and citrus reticulata (blanco) was investigated through intensive sampling. eight primary and four secondary parasitoids, and two p ...200312762866
without or without pheromone habituation: possible differences between insect orders?habituation to sex pheromones is one of the key mechanisms in mating disruption, an insect control tactic. male moths often show reduced sexual response after pre-exposure to female sex pheromone. mating disruption is relatively rare in insect orders other than lepidoptera.201729239513
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