reclassification of shewanella putrefaciens owen's genomic group ii as shewanella baltica sp. nov.the taxonomic relationship between several shewanella putrefaciens isolates from the baltic sea and reference strains of this species is presented in this study. results from dna-dna hybridization using a newly developed non-radioactive detection system and from 16s rrna gene sequencing demonstrated that s. putrefaciens is a heterogeneous species containing more than a single genomic group. the genomic group ii was phylogenetically, genotypically and phenotypically distant enough from the specie ...19989542087
phylogeny and abundance of novel denitrifying bacteria isolated from the water column of the central baltic sea.the baltic sea is an estuarine ecosystem where denitrification in the low oxic and anoxic parts of the deep water contributes significantly to the nitrogen budget. seventy-six heterotrophic, denitrifying, strains have been isolated by four cultivation procedures from the water column of the gotland deep, the main anoxic basin of the central baltic. phylogenetic positions of representative strains of 10 different genotypes, grouped beforehand by low molecular weight (lmw) rna profiling, were esti ...200112024255
shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxic-anoxic interface of the gotland deep in the central baltic sea.three strains of denitrifying estuarine bacteria, os217(t), 05220 and os226, were characterized for their physiological and biochemical features, fatty acid profiles and their phylogenetic position based on 16s rdna sequences. the strains were isolated from the oxic-anoxic interface of an anoxic basin of the central baltic sea. phylogenetic analyses of the 16s rdna sequences revealed a clear affiliation with members of the genus shewanella of the gamma-proteobacteria. the closest sequence simila ...200212508890
shewanella profunda sp. nov., isolated from deep marine sediment of the nankai trough.a novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain lt13a(t)) was isolated from a deep sediment layer in the nankai trough (leg 190, ocean drilling program) off the coast of japan. this organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. max ...200415545415
identification of shewanella baltica as the most important h2s-producing species during iced storage of danish marine fish.shewanella putrefaciens has been considered the main spoilage bacteria of low-temperature stored marine seafood. however, psychrotropic shewanella have been reclassified during recent years, and the purpose of the present study was to determine whether any of the new shewanella species are important in fish spoilage. more than 500 h2s-producing strains were isolated from iced stored marine fish (cod, plaice, and flounder) caught in the baltic sea during winter or summer time. all strains were id ...200516269698
shewanella hafniensis sp. nov. and shewanella morhuae sp. nov., isolated from marine fish of the baltic sea.two novel species belonging to the genus shewanella are described on the basis of their phenotypic characteristics, phylogenetic analyses of 16s rrna and gyrb gene sequences and levels of dna-dna hybridization. a total of 47 strains belonging to two novel gram-negative, psychrotolerant, h2s-producing bacterial species were isolated from marine fish (cod and flounder) caught from the baltic sea off denmark. the phenotypic characteristics of strains belonging to group 1 (14 strains) indicated that ...200616403893
enhancement of polyunsaturated fatty acid production by tn5 transposon in shewanella baltica.transposon tn5 mutagenesis was used to generate random mutations in shewanella baltica mac1, a polyunsaturated fatty acid (pufa)-producing bacterium. three mutants produced 3-5 times more eicosapentaenoic acid (epa 20:5 n-3) compared to the wild type at 10 degrees c. one of the mutants produced 0.3 mg epa g(-1) when grown at high temperature (30 degrees c). moreover, 2 mg docosahexaenoic acid (dha 22:6 n-3) g(-1) was produced by s. baltica mutants at 4 degrees c. sequencing of insertion mutation ...200616799757
investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods.water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ...201020587384
recombinant production of omega-3 fatty acids in escherichia coli using a gene cluster isolated from shewanella baltica isolate eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) genes from shewanella baltica mac1 and to examine recombinant production of epa and dha in e. coli to investigate cost-effective, sustainable and convenient alternative sources for fish oils.201020666868
seafood quality analysis: molecular identification of dominant microbiota after ice storage on several general growth media.this study points out the limitations of several general growth media frequently used in seafood research by a systematic identification of the microorganisms on fish samples during ice storage unable to grow on those media. aerobic psychrotrophic count (apc), replication on various general media and total cultivable microbial community denaturing gradient gel electrophoresis (dgge) analysis revealed that many potential spoilage microorganisms were overlooked. those microorganisms overlooked by ...201121645815
evaluation of the spoilage potential of bacteria isolated from spoiled raw salmon (salmo salar) fillets stored under modified atmosphere packaging.the spoilage potential of eight bacterial groups/species (serratia spp., hafnia alvei, brochothrix thermosphacta, carnobacterium maltaromaticum, shewanella baltica, lactococcus piscium, photobacterium phosphoreum, "other enterobacteriaceae" [containing one strain of moellerella sp., morganella sp. and pectobacterium sp.]) isolated from spoiled raw salmon fillets stored under modified atmosphere packaging (map) was evaluated by inoculation into sterile raw salmon cubes followed by storage for 12d ...201223290229
the microbiota of freshwater fish and freshwater niches contain omega-3 fatty acid-producing shewanella species.approximately 30 years ago, it was discovered that free-living bacteria isolated from cold ocean depths could produce polyunsaturated fatty acids (pufa) such as eicosapentaenoic acid (epa) (20:5n-3) or docosahexaenoic acid (dha) (22:6n-3), two pufa essential for human health. numerous laboratories have also discovered that epa- and/or dha-producing bacteria, many of them members of the shewanella genus, could be isolated from the intestinal tracts of omega-3 fatty acid-rich marine fish. if bacte ...201526497452
histamine-producing bacteria in blue scad (decapterus maruadsi) and their abilities to produce histamine and other biogenic amines.using decarboxylation medium and 16s rdna sequence analysis, histamine-producing bacteria (hpb) in blue scad (decapterus maruadsi) were isolated and identified, and the histamine-producing abilities of the isolated hpb were determined. nine mesophilic strains (h1-h9) isolated from the muscle of blue scad were identified as the genera of hpb, including arthrobacter bergeri (h1), pseudomonas sp. (h2, h5 and h6), psychrobacter sp. (h3), shewanella baltica (h4 and h7), and aeromonas salmonicida (h8 ...201424668182
evaluation of the spoilage potential of bacteria isolated from spoiled cooked whole tropical shrimp (penaeus vannamei) stored under modified atmosphere packaging.the spoilage potential of isolates belonging to five bacterial groups/species (shewanella baltica, carnobacterium maltaromaticum, aeromonas salmonicida, vibrio sp., "other gamma-proteobacteria" [containing one strain of pseudoalteromonas sp. and one strain of psychrobacter sp.]) isolated from spoiled cooked and whole tropical shrimp stored under modified atmosphere packaging (map) was evaluated by inoculation into ionized cooked and peeled tropical shrimp followed by storage for 32 days at 8 °c. ...201424549192
[fatty acid composition alteromonas-like bacteria isolated from the black sea water].alteromonas macleodii strains isolated from the black sea water were similar in their fatty acids composition with the type strain of this species. analysis of lipid composition of 10 a. macleodii strains isolated from the deep and surface water layers in different world ocean regions including the black sea water has shown that the deep and surface isolates of this species formed two groups different in their fatty acids profiles. the black sea isolates of pseudoalteromonas haloplanktis, p. cit ...201526638484
in vitro antibacterial analysis of phenoloxidase reaction products from the sea cucumber apostichopus japonicus.three phenoloxidases (pos) of apostichopus japonicus, ajpos (ajpo1, ajpo2 and ajpo3), were partially purified from the coelomocytes with an electrophoretic method, and then employed for the in vitro antibacterial analysis. using l-3,4-dihydroxyphenylalanine (l-dopa) as a substrate, ajpo1 and ajpo2-derived compounds inhibited the growth of vibrio splendidus and staphylococcus aureus, while ajpo3-derived compounds only inhibited the growth of v. splendidus. when dopamine was used as a substrate, a ...201424931626
spoilage potential characterization of shewanella and pseudomonas isolated from spoiled large yellow croaker (pseudosciaena crocea).ten strains were isolated from a spoiled large yellow croaker (pseudosciaena crocea). all of them were able to grow aerobically from 4 to 30°c, and reduce trimethylamine-n-oxide to trimethylamine (tma) and produce h2 s except sb01, pf05 and pf07. biochemical characterization and phylogenetic analysis of 16s rrna gene showed that eight h2 s-producing isolates were closely related to shewanella baltica, and two isolates pf05 and pf07 were identified as pseudomonas fluorescens and pseudomonas fragi ...201727747903
identification and quantification of acylated homoserine lactones in shewanella baltica, the specific spoilage organism of pseudosciaena crocea, by ultrahigh-performance liquid chromatography coupled to triple quadrupole mass spectrometry.bacteria communicate with one another using chemical signal molecules called autoinducers, and the most prevalent autoinducers used by gram-negative bacteria are n-acylated homoserine lactones (ahls). however, ahls are hardly detected in shewanella baltica, the specific spoilage organism of pseudosciaena crocea. in this study, we applied ultrahigh-performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry to determine ahls. this method enabled the quantification of u ...201728535043
inhibition of biofilm development and spoilage potential of shewanella baltica by quorum sensing signal in cell-free supernatant from pseudomonas fluorescens.the objective of this study was to in vitro evaluate the effect of a cell-free supernatant (cfs) containing quorum sensing (qs) signal of pseudomonas fluorescens on the growth, biofilm development and spoilage potential of shewanella baltica, and preliminarily assess the interactive influences of various chemically synthesized autoinducers on spoilage phenotypes of s. baltica. pf01 strain isolated from spoiled pseudosciaen crocea was identified p. fluorescens. the addition of 25% and 50% cfs to ...201627149651
high-resolution crystal structure of the recombinant diheme cytochrome c from shewanella baltica (os155).multiheme cytochromes c (cyts c) are c-type cyts characterized by non-standard structural and spectroscopic properties. the relative disposition of the heme cofactors in the core of these proteins is conserved and they can be classified from their geometry in two main groups. in one group the porphyrin planes are arranged in a perpendicular fashion, while in the other they are parallel. orientation of the heme groups is a key factor that regulates the intramolecular electron transfer pathway. a ...201524559494
transformation of shewanella baltica with cole1-like and p1 plasmids and their maintenance during bacterial growth in cultures.the presence of natural plasmids has been reported for many shewanella isolates. however, knowledge about plasmid replication origin and segregation mechanisms is not extensive for this genus. shewanella baltica is an important species in the marine environment due to its denitrification ability in oxygen-deficient zones and the potential role in bioremediation processes. however, no information about possible use of plasmid vectors in this species has been reported to date. here we report that ...201526170108
cloning, expression, and physicochemical characterization of a new diheme cytochrome c from shewanella baltica os155.the 16-kda diheme cytochrome c from the bacterium shewanella baltica os155 (sb-dhc) was cloned and expressed in escherichia coli and investigated through uv-vis, magnetic circular dichroism, and (1)h nmr spectroscopies and protein voltammetry. the model structure was obtained by means of comparative modeling using the x-ray structure of rhodobacter sphaeroides diheme cytochrome c (rs-dhc) (with a 37% pairwise sequence identity) as a template. sb-dhc folds into two distinct domains, each containi ...201021161306
[the effects of the humic substances on azoreduction by shewanella spp].under anaerobic conditions, shewanella cinicad14(t), shewanella baltica and shewane-lla putrefaciens are capable of high-rate azoreduction and humus reduction. the results indicated that at low concentration ( <2 mmol/l) aqs was a accelerator for bacterial azoreduction. however, when the concentration of aqs was more than 5 mmol/l a strong inhibition was occurred. on the other hand, the concentration of aqds as high as 12 mmol/l the inhibition of azoreduction was still not exhibited, but the eff ...200617037061
shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant.a highly efficient dye-decolorizing bacterium, strain s12t, was isolated from activated sludge of a textile-printing waste-water treatment plant in guangzhou, china. the cells were gram-negative and motile by means of a single polar flagellum. the strain was capable of anaerobic growth either by fermentation of glucose or by anaerobic respiration and utilized a variety of electron acceptors, including nitrate, iron oxide and thiosulfate. the physiological properties, tested by using the biolog g ...200515653901
influence of acetate and co2 on the tmao-reduction reaction by shewanella this work, the tmao-reduction by shewanella baltica, one of the representative spoilage organisms in modified atmosphere packaged marine fish fillets, and the effect of acetate and co2 on this reduction were studied in vitro. the growth of s. baltica and the corresponding evolution of some compounds (acetate, lactate, pyruvate, glucose and trimethylamine (tma)) were followed during storage at 4 degrees c in two types of broths. the first medium was a defined medium (ph = 6.8) to which lactate ...200111545211
genome sequencing of five shewanella baltica strains recovered from the oxic-anoxic interface of the baltic we describe five shewanella baltica genomes recovered from the same sample, as well as 12 years apart from the same sampling station. these genomes expand the collection of previously sequenced s. baltica strains and represent a valuable resource for assessing the role of environmental settings on genome adaptation.201222328742
identification and characterization of extracellular cyclic dipeptides as quorum-sensing signal molecules from shewanella baltica, the specific spoilage organism of pseudosciaena crocea during 4 °c storage.quorum-sensing (qs) signaling molecules are able to mediate specific gene expression inside spoilage bacteria in response to population density and thus are implicated in food spoilage. in the present work, a total of 102 strains of spoilage bacteria were isolated from pseudosciaena crocea at 4 °c storage, and of these, 60 strains were identified as shewanella spp., and 48 strains (47.1%) were identified as s. baltica. in addition, the spoilage capabilities of three different s. baltica strains ...201324206027
stability, genotypic and phenotypic diversity of shewanella baltica in the redox transition zone of the baltic sea.studying how bacterial strains diverge over space and time and how divergence leads to ecotype formation is important for understanding structure and dynamics of environmental communities. here we assess the ecological speciation and temporal dynamics of a collection of shewanella baltica strains from the redox transition zone of the central baltic sea, sampled at three time points over a course of 12 years, with a subcollection containing 46 strains subjected to detailed genetic and physiologic ...201424286373
phenotypic and genotypic characterization of h2 s-positive and h2 s-negative strains of shewanella baltica isolated from spoiled whiting (merlangius merlangus).four strains were isolated from a spoiled whiting (merlangius merlangus). all of them were able to grow aerobically from 4 to 30°c and also able to develop anaerobically in the presence of trimethylamine n-oxide (tmao) at 25°c. biochemical characterization did not allow identification of the strains species but showed that one of the four strains was unable to produce h2 s. two strains synthetized an ornithine decarboxylase being potential putrescine producers. results of carbon source use highl ...201425073930
the involvement of bacterial quorum sensing in the spoilage of refrigerated litopenaeus vannamei.quorum-sensing signals in refrigerated shrimp (litopenaeus vannamei) undergoing spoilage were examined using bioreporter assays, thin-layer chromatography and gas chromatography-mass spectrometry, and the results revealed the presence of three types of autoinducers including acetylated homoserine lactones (ahls) (i.e., n-hexanoyl-homoserine lactone, n-oxohexanoyl-homoserine lactone and n-octanoyl-homoserine lactone), autoinducer-2, and cyclic dipeptides (i.e., cyclo-(l-pro-l-leu), cyclo-(l-leu-l ...201525305441
quorum sensing signals affect spoilage of refrigerated large yellow croaker (pseudosciaena crocea) by shewanella this work we investigated the specific spoilage organism (sso) of large yellow croaker (pseudosciaena crocea) stored at 4°c and role of quorum sensing (qs) system of sso isolated from the spoiled fish. according to microbial count and 16s rrna gene of the isolated pure strains, shewanella, mainly shewanella baltica and shewanella putrefaciens, was predominant genera at the end of shelf-life of p. crocea. among shewanella isolates, s.baltica02 was demonstrated as sso in spoilage potential char ...201626519730
inhibition of quorum sensing, biofilm, and spoilage potential in shewanella baltica by green tea polyphenols.we investigated the quorum sensing (qs) system of shewanella baltica and the anti-qs related activities of green tea polyphenols (tp) against spoilage bacteria in refrigerated large yellow croaker. autoinducer-2 (ai-2) and the diketopiperazines (dkps) cyclo-(l-pro-l-leu) and cyclo-(l-pro-l-phe) were detected in the culture extract of s. baltica xh2, however, no n-acylhomoserine lactones (ahls) activity was observed. green tp at sub-inhibitory concentrations interfered with ai-2 and dkps activiti ...201526626353
draft genome sequence of shewanella baltica m1 isolated from brackish surface water of the gulf of gdań, we present the 5.168-mbp draft genome sequence of shewanella baltica m1, the first shewanella strain from the gulf of gdańsk to have its genome sequenced and annotated. the availability of the genome sequence of strain m1 will promote further global analyses of bacterial stress responses in the unique gulf of gdańsk ecosystem.201627340075
shewanella baltica ecotypes have wide transcriptional variation under the same growth bacterial populations, subtle expressional differences may promote ecological specialization through the formation of distinct ecotypes. in a barrier-free habitat, this process most likely precedes population divergence and may predict speciation events. to examine this, we used four sequenced strains of the bacterium shewanella baltica, os155, os185, os195, and os223, as models to assess transcriptional variation and ecotype formation within a prokaryotic population. all strains were isolate ...201727777983
chitin oligosaccharide deacetylase from shewanella baltica atcc baa-1091.chitin oligosaccharide deacetylase (cod) from bacteria that have been examined so far typically comprise two carbohydrate-binding domains (cbds) and one polysaccharide deacetylase domain. in contrast, shewanella baltica atcc baa-1091 cod (sb-cod) has only one cbd, yet exhibits chitin-binding properties and substrate specificities similar to those of other cods.201727832730
kinetics of biofilm formation and desiccation survival of listeria monocytogenes in single and dual species biofilms with pseudomonas fluorescens, serratia proteamaculans or shewanella baltica on food-grade stainless steel surfaces.this study investigated the dynamics of static biofilm formation (100% rh, 15 °c, 48-72 h) and desiccation survival (43% rh, 15 °c, 21 days) of listeria monocytogenes, in dual species biofilms with the common spoilage bacteria, pseudomonas fluorescens, serratia proteamaculans and shewanella baltica, on the surface of food grade stainless steel. the gram-negative bacteria reduced the maximum biofilm population of l. monocytogenes in dual species biofilms and increased its inactivation during desi ...201324102145
engineering of epa/dha omega-3 fatty acid production by lactococcus lactis subsp. cremoris mg1363.eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) have been shown to be of major importance in human health. therefore, these essential polyunsaturated fatty acids have received considerable attention in both human and farm animal nutrition. currently, fish and fish oils are the main dietary sources of epa/dha. to generate sustainable novel sources for epa and dha, the 35-kb epa/dha synthesis gene cluster was isolated from a marine bacterium, shewanella baltica mac1. to streamline the i ...201424389665
dha production in escherichia coli by expressing reconstituted key genes of polyketide synthase pathway from marine bacteria.the gene encoding phosphopantetheinyl transferase (pptase), pfae, a component of the polyketide synthase (pks) pathway, is crucial for the production of docosahexaenoic acid (dha, 22:6ω3), along with the other pfa cluster members pfaa, pfab, pfac and pfad. dha was produced in escherichia coli by co-expressing pfaabcd from dha-producing colwellia psychrerythraea 34h with one of four pfae genes from bacteria producing arachidonic acid (ara, 20:4ω6), eicosapentaenoic acid (epa, 20:5ω3) or dha, resp ...201627649078
[influence of environmental factors on electricity production by microbial fuel cell inoculation shewanella baltica].the influences of the anodic substance, concentration, ph and temperature on the electricity production by mfcs were discussed. the lactic sodium was better than acetic sodium or glucose. the power density of mfc and the concentration of lactic sodium were well filled with monod model. the power density was 1236 mw/m2 when the ph of anodic chamber was 8. the power density of mfc increased with the ph increasing from 6 to 8, which was due to the anodic internal resistance. the power density was 1 ...200919775023
microflora assessments using pcr-denaturing gradient gel electrophoresis of ozone-treated and modified-atmosphere-packaged farmed cod fillets.denaturing gradient gel electrophoresis (dgge) of a pcr-amplified 16s rdna sequence was used to characterize changes in the microbial flora caused by ozone (o3) treatment of farmed cod (gadus morhua). portions of cod were produced under controlled conditions, bathed in fresh water supplemented with 2 ppm of o3 for 30 min, and packaged in modified atmosphere (ma: 60% co2 and 40% n2) before 4 degrees c storage. control samples were packaged in ma or air, without prior o3 treatment. samples were an ...200718044421
unprecedented levels of horizontal gene transfer among spatially co-occurring shewanella bacteria from the baltic sea.high-throughput sequencing studies during the last decade have uncovered that bacterial genomes are very diverse and dynamic, resulting primarily from the frequent and promiscuous horizontal gene exchange that characterizes the bacterial domain of life. however, a robust understanding of the rates of genetic exchange for most bacterial species under natural conditions and the influence of the ecological settings on the rates remain elusive, severely limiting our view of the microbial world. here ...201120596068
Displaying items 1 - 41 of 41