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enterosistem 18-r: description and comparative evaluation with conventional methods for identification of members of the family enterobacteriaceae.the efficiency and accuracy of enterosistem 18-r (liofilchem s.r.l., roseto degli abruzzi, teramo, italy) were compared with those of conventional biochemical methods to identify 360 members (38 species) of the family enterobacteriaceae. overall, 329 strains (91.3%) were correctly identified (percentage of identification, greater than or equal to 90.0), with 37 (11.2%) requiring additional tests for complete identification. for 11 isolates (3.1%), enterosistem 18-r gave only genus identification ...19911939588
aztreonam in the treatment of bone and joint infections caused by gram-negative bacilli.aztreonam was used successfully in 17 of 17 patients with orthopedic infections due to gram-negative bacilli (11, osteomyelitis; six, septic arthritis). duration of treatment ranged from 14 to 55 days, and the period of follow-up was four to 18 months. causative organisms included pseudomonas aeruginosa, serratia marcescens, enterobacter gergoviae, citrobacter diversus, proteus mirabilis, and enterobacter aerogenes. aztreonam was well tolerated. the only definite reactions attributable to aztreo ...19852934786
enterobacter asburiae sp. nov., a new species found in clinical specimens, and reassignment of erwinia dissolvens and erwinia nimipressuralis to the genus enterobacter as enterobacter dissolvens comb. nov. and enterobacter nimipressuralis comb. nov.enterobacter asburiae sp. nov. is a new species that was formerly referred to as enteric group 17 and that consists of 71 strains, 70 of which were isolated from humans. enterobacter asburiae sp. nov. strains gave positive reactions in tests for methyl red, citrate utilization (simmons and christensen's), urea hydrolysis, l-ornithine decarboxylase, growth in kcn, acid and gas production from d-glucose, and acid production from l-arabinose, cellobiose, glycerol (negative in 1 to 2 days, positive ...19863711302
treatment of gram-negative infections with aztreonam.twenty-one patients with serious gram-negative infections were treated with aztreonam. twenty of these were clinical and microbiologic cures; there was one clinical improvement with microbiologic persistence. no bacteria became resistant. cure rates were: bone and joint (11 of 11); skin and soft tissue (six of six); pneumonia (two of two); perinephric abscess (one of one); and intra-abdominal abscess (zero of one). the bacteria responsible for these infections included pseudomonas aeruginosa (12 ...19854038577
[study of the species "enterobacter gergoviae" by dna/dna hybridization (author's transl)].a dna/dna hybridization study was carried out with 13 strains of enterobacter gergoviae and 123 strains of enterobacteriaceae, mostly enterobacter and klebsiella species. e. gergoviae strains were very highly related (relative binding ratios: 88-96%) to the type strain cip 76-01, as previously reported by brenner and co-workers. no close genomic relationship was found with other species of enterobacter and klebsiella (mean relative binding ratio: 39%) and other enterobacteriaceae.19827103307
taxonomic diversity of anaerobic glycerol dissimilation in the enterobacteriaceae.a total of 1,123 strains representing 128 taxa in the enterobacteriaceae (named species or subspecies and genomic species) were screened for the presence of glycerol dehydrogenases and 1,3-propanediol dehydrogenase. only eight taxa, citrobacter freundii sensu stricto, c. youngae, c. braakii, c. werkmanii, citrobacter genomospecies 10 and 11, enterobacter gergoviae and klebsiella pneumoniae subsp. pneumoniae could grow fermentatively on glycerol and possessed both glycerol dehydrogenase type i (i ...19957569322
extended-spectrum beta-lactamases in clinical isolates of enterobacter gergoviae and escherichia coli in china.resistance to ceftazidime, detected in isolates of escherichia coli 5518 and enterobacter gergoviae 3773 from our hospital, was transferred, together with resistance to aminoglycosides, trimethoprim, sulfonamide, and other beta-lactam antibiotics, by conjugation to e. coli jp559. both e. coli transconjugants were resistant to ampicillin, all cephalosporins, and aztreonam but remained susceptible to cefoxitin and imipenem. the enzymes of the two transconjugant strains readily hydrolyzed cephalosp ...19947695270
[nucleotide sequence analysis of shv-2 beta-lactamase gene in enterobacter gergoviae].the extended-spectrum beta-lactamase (esbla) gene on r-plasmid harbored in e. coli c3773, a transconjugant of the clinical isolate of ent. gergoviae 3773 from our hospital, was cloned and two fragments were subcloned into m13. the nucleotide sequences of two fragments were determined. the primary structure of this esbla deduced from nucleotide sequence had only a gly-->ser change at position 234 in comparison with shv-1, which was supposed to be responsible for extended-spectrum activity and res ...19947975558
[a plasmid-mediated shv type extended-spectrum beta-lactamase in beijing isolate of enterobacter gergoviae].by conjugation experiment, an extended-spectrum beta-lactamase (esb1a) encoded on a transferable plasmid of approximately 60 kilobases was found in a clinical strain of enterobacter gergoviae isolated from peking union medical college hospital. its e. coli transconjugant was resistant to all beta-lactams tested except cefoxitin and imipenem. clavulanic acid, a classical beta-lactamase inhibitor, inhibited its activity. hybridization with an intragenic probe for shv-1 revealed that this esb1a may ...19948073758
transformation of enterobacter gergoviae isolated from pink bollworm (lepidoptera: gelechiidae) gut with bacillus thuringiensis toxin.production of molecules with toxic activity by genetically transformed symbiotic bacteria of pest insects may serve as a powerful approach to biological control. the symbiont, enterobacter gergoviae, isolated from the gut of the pink bollworm (pbw), has been transformed to express cyt1a, a cytolytic protein toxin lethal to mosquito and black fly larvae, as a model system. these transgenic bacteria might be used to spread genes encoding insecticidal proteins to populations of agricultural insects ...200211727033
epidemiology of extended-spectrum beta-lactamase-producing enterobacter isolates in a spanish hospital during a 12-year period.fifteen enterobacter clinical isolates (11 enterobacter cloacae isolates, 3 enterobacter aerogenes isolates, and 1 enterobacter gergoviae isolate), representing 0.4% of all enterobacter isolates recovered in our hospital from 1989 to 2000, were suspected of harboring an extended-spectrum beta-lactamase (esbl). these isolates were recovered from 14 different patients. esbls were transferred by conjugation into an escherichia coli recipient strain. pulsed-field gel electrophoresis (pfge) revealed ...200211923338
[enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital].to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms.200211939120
prba, a gene coding for an esterase hydrolyzing parabens in enterobacter cloacae and enterobacter gergoviae strains.the new gene prba encodes an esterase responsible for the hydrolysis of the ester bond of parabens in enterobacter cloacae strain em. this gene is located on the chromosome of strain em and was cloned by several pcr approaches. the prba gene codes for an immature protein of 533 amino acids, the first 31 of which represent a proposed signal peptide yielding a mature protein of a putative molecular mass of 54.6 kda. this enzyme presents analogies with other type b carboxylesterases, mainly of euka ...200212193616
natural antibiotic susceptibility of enterobacter amnigenus, enterobacter cancerogenus, enterobacter gergoviae and enterobacter sakazakii strains.to investigate the natural susceptibility to 69 antimicrobial agents of 107 enterobacter strains comprising e. amnigenus (n = 18), e. cancerogenus (n = 26), e. gergoviae (n = 28) and e. sakazakii (n = 35).200212427217
nosocomial outbreak of enterobacter gergoviae bacteraemia in a neonatal intensive care unit.a nosocomial outbreak of bacteraemia, caused by enterobacter gergoviae infected 11 babies, nine of whom were premature, and was investigated in the neonatal intensive care unit (nicu) of a general hospital in johor bahru, malaysia. the strain that was isolated from the babies was also isolated from the dextrose saline used for the dilution of parenteral antibiotics and from the hands of a healthcare worker on duty in the nursery. pulsed-field gel electrophoresis (pfge) of xba i-digested chromoso ...200312660126
detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of enterobacter spp. in hong kong, 2000-2002.a total of 139 consecutive and non-duplicate bloodstream isolates of enterobacter spp. collected from inpatients in hong kong during 2000-2002 were studied for production of extended-spectrum beta-lactamases (esbls).200515681579
temporal association of entomopathogenic nematodes (rhabditida: steinernematidae and heterorhabditidae) and bacteria.galleria mellonella l. larvae were infected with three species (seven strains) of steinernema spp. or three species (three strains) of heterorhabditis spp. infected larvae were incubated at 22, 27, and 32 degrees c. larvae were dorsally dissected every 6h over a 48-h period. hemolymph was collected and streaked on tryptic soy agar plates. several non-symbiotic bacterial species were identified from infected insect cadavers: enterobacter gergoviae, vibrio spp., pseudomonas fluorescens type c, ser ...200616448667
enterobacter gergoviae and the prevalence of efflux in parabens resistance.in order to characterize the mechanism involved in parabens resistance, we studied 13 enterobacter gergoviae collected from diverse cosmetic formulations containing parabens as preservatives and 10 isolates from clinical or industrial sources.200616473920
[lower respiratory tract infection due to enterobacter gergoviae]. 200516498735
constitutive expression of the nifa gene activates associative nitrogen fixation of enterobacter gergoviae 57-7, an opportunistic endophytic diazotroph.this study was undertaken to investigate whether a nitrogen-fixing bacterium enterobacter gergoviae 57-7, which was isolated from surface-sterilized maize (zea mays l.) roots, can colonize in roots and whether constitutive expression of the nifa gene encoding the transcriptional activator of nitrogenase genes can activate nif gene expression in planta.200717714394
survey of micro-organisms associated with spoilage of cosmetic creams manufactured in south africa.synopsis two studies were conducted to determine the predominant micro-organisms found in spoiled cosmetic creams manufactured in south africa. the products evaluated included facial creams and hand and body lotions. spoiled cosmetic creams were obtained from different manufacturers and analysed microbiologically for the presence of bacteria, yeasts and moulds. the frequency and relative density (percentage) of micro-organisms isolated from naturally spoiled creams were calculated. pseudomonas a ...199619245476
traumatic endophthalmitis caused by enterococcus raffinosus and enterobacter gergoviae.we report a rare case of polymicrobial traumatic endophthalmitis caused by enterococcus raffinosus and enterobacter gergoviae with successful interventions of complete pars plana vitrectomy with intravitreal injections of vancomycin and amikacin. in addition, the patient achieved a favourable visual acuity of 20/200.200919273651
synthesis of 9-beta-d-arabinofuranosylguanine by combined use of two whole cell biocatalysts.unlike the preparation of other purine nucleosides, transglycosylation from a pyrimidine nucleoside and guanine is difficult because of the low solubility of this base. thus, another strategy, based on the coupled action of two whole cell biocatalyzed reactions, transglycosylation and deamination, was used. enterobacter gergoviae and arthrobacter oxydans were employed to synthesize 9-beta-d-arabinofuranosylguanine (arag), an efficient anti leukemic drug.200919523826
coliform accumulation in amphibalanus amphitrite (darwin, 1854) (cirripedia) and its use as an organic pollution bioindicator in the estuarine area of recife, pernambuco, brazil.samples of water and barnacles amphibalanus amphitrite were collected from recife, brazil, to assess if it accumulates total (tc) and thermotolerant coliforms (ttc) related with sewage pollution. the most probable number (mpn) values and the standard procedures for examination of shellfish were used. comparatively with the water samples, the highest coliform values came from the barnacles, with tc values ranging from < 3.0 x 10(3) to > 2.4 x 10(6) mpn.g-1, and ttc ranging from > 2.4 x 10(3) to 2 ...201020549061
antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket).the aim of this study was to elucidate whether and to what extent fresh produce from germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. for this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in germany and examined bacteriologically. phenotypic resistance of enterobacter cloacae (n=172); enterobacter gergoviae (n=92); pantoea agglomerans (n=96); pseudomonas aeruginosa (n=295); pseudom ...201121700353
antimicrobial and herbal drug resistance in enteric bacteria isolated from faecal droppings of common house lizard/gecko (hemidactylus frenatus).from 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (ifs,18) and hostels, guest houses, and dining rooms of different canteen/mess (hgm, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. escherichia coli were the most frequently (39) isolated followed by citrobacter freundii (33), klebsiella pneumonia (27), salmonella indica (12), enterobacter gergoviae (12), and ent. agglomeran ...201324223595
microbiological study of therapeutic soft contact lenses used in the treatment of recurrent corneal erosion syndrome.to determine the bacteriological spectrum of the removed therapeutic soft contact lenses (tscls) and to establish efficacy of prophylactic antibiotics on tscls used for 2 weeks for treatment of patients with recurrent corneal erosion syndrome (rces).201525230080
tension pneumatocele due to enterobacter gergoviae pneumonia: a case report.pneumatocele formation is a known complication of pneumonia. very rarely, they may increase markedly in size, causing cardiorespiratory compromise. many organisms have been implicated in the pathogenesis of this disease; however, this is the first report of tension pneumatocele resulting from enterobacter gergoviae pneumonia. we report a case of a 3-month-old nigerian male child who developed two massive tension pneumatoceles while on treatment for postpneumonic empyema due to enterobacter gergo ...201223056055
enterobacter gergoviae adaptation to preservatives commonly used in cosmetic industry.the aim of this study was to obtain a better understanding regarding the origin of recurrent contamination by enterobacter gergoviae in diverse cosmetic formula. we studied 65 isolates collected from various sources (clinical, food, cosmetics).201424828151
increasing antibiotic resistance in preservative-tolerant bacterial strains isolated from cosmetic products.to ensure the microbiological quality, consumer safety and organoleptic properties of cosmetic products, manufacturers need to comply with defined standards using several preservatives and disinfectants. a drawback regarding the use of these preservatives is the possibility of generating cross-insusceptibility to other disinfectants or preservatives, as well as cross resistance to antibiotics. therefore, the objective of this study was to understand the adaptive mechanisms of enterobacter gergov ...201526415667
septic shock caused by klebsiella pneumoniae carbapenemase-producing enterobacter gergoviae in a neutropenic patient with leukemia.we present the first reported infection caused by klebsiella pneumoniae carbapenemase (kpc)-producing enterobacter gergoviae. the patient had leukemia and neutropenia and died of septic shock from kpc-producing e. gergoviae bacteremia. the emergence of kpcs in additional species of enterobacteriaceae is alarming and may disproportionately affect patients with hematologic malignancies.201323761145
carbapenem-resistant enterobacter gergoviae harbouring blakpc-2 in brazil. 201425129316
enterobacter gergoviae membrane modifications are involved in the adaptive response to preservatives used in cosmetic industry.the objective of this study was to understand the adaptive mechanisms in enterobacter gergoviae which are involved in recurrent contaminations in cosmetic products that are incorporated with preservatives.201525355161
complete genome sequence of pluralibacter gergoviae fb2, an n-acyl homoserine lactone-degrading strain isolated from packed fish paste.pluralibacter gergoviae fb2, a bacterial strain isolated from packed food, has been found to exhibit quorum-quenching properties. hence, we report the first, complete genome of p. gergoviae sequenced using the pacific biosciences single-molecule, real-time (smrt) platform.201425502672
phenotypic changes contributing to enterobacter gergoviae biocide resistance.enterobacter gergoviae is a recurrent contaminant of cosmetic and hygiene products. to understand how this bacterium adapts to biocides, we studied ent. gergoviae cip 76.01 and its triclosan and methylisothiazolinone-chloromethylisothiazolinone (mit-cmit) tolerant isogenic mutants. they were compared with others also isolated from contaminated cosmetics. phenotypic differences were noted and these included changes in the bacterial envelope and flagella along with differences in motility, and bio ...201525919899
outbreak of imp-producing carbapenem-resistant enterobacter gergoviae among kidney transplant recipients.the objective of this study was to investigate a prolonged outbreak of carbapenem-resistant enterobacter gergoviae (creg) involving kidney transplant recipients (ktrs) between 2009 and 2014.201627197663
two symbiotic bacteria of the entomopathogenic nematode heterorhabditis spp. against galleria mellonella.the entomopathogenic nematode heterorhabditis spp. is considered a promising agent in the biocontrol of injurious insects of agriculture. however, different symbiotic bacteria associated with the nematode usually have different specificity and virulence toward their own host. in this study, two symbiotic bacteria, ly2w and nk, were isolated from the intestinal canals of two entomopathogenic nematode heterorhabditis megidis 90 (pdsj1 and pdsj2) from galleria mellonela, separately. to determine th ...201727889603
taxonomic evaluation of the genus enterobacter based on multilocus sequence analysis (mlsa): proposal to reclassify e. nimipressuralis and e. amnigenus into lelliottia gen. nov. as lelliottia nimipressuralis comb. nov. and lelliottia amnigena comb. nov., respectively, e. gergoviae and e. pyrinus into pluralibacter gen. nov. as pluralibacter gergoviae comb. nov. and pluralibacter pyrinus comb. nov., respectively, e. cowanii, e. radicincitans, e. oryzae and e. arachidis into kosakonia gen. nov. as kosakonia cowanii comb. nov., kosakonia radicincitans comb. nov., kosakonia oryzae comb. nov. and kosakonia arachidis comb. nov., respectively, and e. turicensis, e. helveticus and e. pulveris into cronobacter as cronobacter zurichensis nom. nov., cronobacter helveticus comb. nov. and cronobacter pulveris comb. nov., respectively, and emended description of the genera enterobacter and cronobacter.the taxonomy of enterobacter has a complicated history, with several species transferred to and from this genus. classification of strains is difficult owing to its polyphyletic nature, based on 16s rrna gene sequences. it has been previously acknowledged that enterobacter contains species which should be transferred to other genera. in an attempt to resolve the taxonomy of enterobacter, mlsa based on partial sequencing of protein-encoding genes (gyrb, rpob, infb and atpd) was performed on the t ...201323632228
laryngoscopes: evaluation of microbial load of blades.laryngoscope blades were analyzed, and the presence of blood, bodily fluids, and microorganisms was verified, indicating their potential as a source of cross contamination during clinical usage. the way in which the blades are cleaned and disinfected in daily practice may place the patient and health care team at risk. the aim of this study was to determine the bacterial and fungal load on this equipment.201626775934
enterobacteriaceae isolates from the oral cavity of workers in a brazilian oncology hospital.the evaluation of workers as potential reservoirs and disseminators of pathogenic bacteria has been described as a strategy for the prevention and control of healthcare-associated infections (hais). the aim of this study was to evaluate the presence of enterobacteriaceae in the oral cavity of workers at an oncology hospital in the midwest region of brazil, as well as to characterize the phenotypic profile of the isolates. saliva samples of 294 workers from the hospital's healthcare and support t ...201525923890
effect of a chronic nail-biting habit on the oral carriage of enterobacteriaceae.nail biting is a common oral habit in children and young adults. however, its effect on the oral carriage of enterobacteriaceae is unclear. the purpose of the study was to evaluate the differences in prevalence of enterobacteriaceae in saliva samples from subjects with and without a nail-biting habit.200717241163
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