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development of a cost-effective method for capripoxvirus genotyping using snapback primer and dsdna intercalating dye.sheep pox virus (sppv), goat pox virus (gtpv) and lumpy skin disease virus (lsdv) are very closely related viruses of the capripoxvirus (capv) genus of the poxviridae family. they are responsible for sheep pox, goat pox and lumpy skin disease which affect sheep, goat and cattle, respectively. the epidemiology of capripox diseases is complex, as some capvs are not strictly host-specific. additionally, the three forms of the disease co-exist in many sub-saharan countries which complicates the iden ...201324116084
transovarial passage and transmission of lsdv by amblyomma hebraeum, rhipicephalus appendiculatus and rhipicephalus decoloratus.lumpy skin disease (lsd), an acute, sub-acute or inapparent disease of cattle, is caused by lumpy skin disease virus (lsdv), a member of the genus capripoxvirus in the family poxviridae. lsd is characterised by high fever, formation of circumscribed skin lesions and ulcerative lesions on the mucous membranes of the mouth, respiratory and digestive tracts. it is an economically important disease due to the permanent damage to hides, the reduction in productivity and trade restrictions imposed on ...201423975564
evidence of lumpy skin disease virus over-wintering by transstadial persistence in amblyomma hebraeum and transovarial persistence in rhipicephalus decoloratus ticks.lumpy skin disease is a debilitating cattle disease caused by the lumpy skin disease virus (lsdv), belonging to the genus capripoxvirus. epidemics of the disease usually occur in summer, when insect activity is high. limited information is available on how lsdv persists during inter-epidemic periods. transmission of lsdv by mosquitoes such as aedes aegypti has been shown to be mechanical, there is no carrier state in cattle and the role of wildlife in the epidemiology of the disease seems to be ...201423975563
evidence of transstadial and mechanical transmission of lumpy skin disease virus by amblyomma hebraeum ticks.lumpy skin disease (lsd) is an economically important disease caused by lsd virus (lsdv), a capripoxvirus, characterized by fever and circumscribed skin lesions. it is suspected to be transmitted mechanically by biting flies. to assess the vector potential of amblyomma hebraeum in transmission of lsdv, mechanical/intrastadial and transstadial modes of transmission of the virus by this tick species were investigated. two cattle were artificially infected as sources (donors) of infection to ticks. ...201523702314
detection of capripoxvirus dna using a novel loop-mediated isothermal amplification assay.sheep poxvirus (sppv), goat poxvirus (gtpv) and lumpy skin disease virus (lsdv) are the most serious poxviruses of ruminants. they are double stranded dna viruses of the genus capripoxvirus, (subfamily chordopoxvirinae) within the family poxviridae. the aim of this study was to develop a loop-mediated isothermal amplification (lamp) assay for the detection of capripoxvirus (capv) dna.201323634704
evidence of vertical transmission of lumpy skin disease virus in rhipicephalus decoloratus ticks.lumpy skin disease (lsd) is an economically important acute or sub-acute disease of cattle that occurs across africa and in the middle east. the aim of this study was to assess whether rhipicephalus decoloratus ticks were able to transmit lumpy skin disease virus (lsdv) transovarially. uninfected, laboratory-bred r. decoloratus larvae were placed to feed on experimentally infected "donor" cattle. after completion of the life cycle on donor animals, fully engorged adult female ticks were harveste ...201323545323
detection of lumpy skin disease virus in saliva of ticks fed on lumpy skin disease virus-infected cattle.lumpy skin disease is an economically important disease of cattle that is caused by the lumpy skin disease virus (lsdv), which belongs to the genus capripoxvirus. it is endemic in africa and outbreaks have also been reported in the middle-east. transmission has mostly been associated with blood-feeding insects but recently, the authors have demonstrated mechanical transmission by rhipicephalus appendiculatus as well as mechanical/intrastadial and transstadial transmission by amblyomma hebraeum. ...201323456606
seminal transmission of lumpy skin disease virus in heifers.it is known that lumpy skin disease virus (lsdv) can be shed in bull semen following infection and also that artificial insemination (ai) poses a biosecurity risk. however, it is not known whether the use of lsdv infected semen in ai poses a biosecurity risk. the aim of this study was to investigate whether lsdv, transmitted through semen, can infect cows and their embryos. two controlled trials were performed simultaneously. eleven young beef heifers, naïve to lsdv, were synchronized using an o ...201423289592
mechanical transmission of lumpy skin disease virus by rhipicephalus appendiculatus male ticks.lumpy skin disease (lsd) is an economically important, acute or sub-acute, viral disease of cattle that occurs across africa and in the middle east. the aim of this study was to investigate if lumpy skin disease virus (lsdv) can be transmitted mechanically by african brown ear ticks (rhipicephalus appendiculatus neum.). laboratory-bred r. appendiculatus males were fed on experimentally infected viraemic 'donor' cattle. partially fed male ticks were then transferred to feed on an uninfected 'reci ...201322717050
modification of two capripoxvirus quantitative real-time pcr assays to improve diagnostic sensitivity and include beta-actin as an internal positive control.capripoxviruses (capvs), consisting of sheeppox virus (spv), goatpox virus (gpv), and lumpy skin disease virus (lsdv) species, cause economically significant diseases in sheep, goats, and cattle, respectively. quantitative real-time polymerase chain reaction (qpcr) assays are routinely used for rapid detection of capvs in surveillance and outbreak management programs. we further modified and optimized 2 previously published capv qpcr assays, referred to as the balinsky and bowden assays, by chan ...201728430087
complete genome sequence of lumpy skin disease virus isolate serbia/bujanovac/2016, detected during an outbreak in the balkan area.the lumpy skin disease virus (lsdv) isolate serbia/bujanovac/2016 consists of 150,661 nucleotides and has a 99.95% nucleotide identity with the neethling warmbaths lw strain isolated in south africa in 1999. this is the first complete lsdv genome determined in serbia and also in the balkan area.201728860246
development and validation of a taqman probe-based real-time pcr method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.lumpy skin disease (lsd) is a transboundary viral disease of cattle with severe economic impact. immunization of cattle with homologous live attenuated vaccines poses a number of diagnostic problems, as it has been associated with adverse reactions resembling disease symptoms. the latter hampers clinical diagnosis and poses challenges in virus identification. to this end, a duplex quantitative real-time pcr method targeting the gpcr gene was developed and validated, for the concurrent detection ...201728837841
complete genome sequence of the lumpy skin disease virus isolated from the first reported case in greece in 2015.lumpy skin disease virus (lsdv) causes an economically important disease in cattle. here, we report the complete genome sequence of the first lsdv isolate identified in mainland europe. lsdv isolate evros/gr/15 was isolated from the first cases reported on 18 august 2015 in the evros region, greece.201728729259
a g-protein-coupled chemokine receptor: a putative insertion site for a multi-pathogen recombinant capripoxvirus vaccine strategy.capripoxviruses (capvs) have been shown to be ideal viral vectors for the development of recombinant multivalent vaccines to enable delivery of immunogenic genes from ruminant pathogens. so far, the viral thymidine kinase (tk) gene is the only gene used to generate recombinants. a putative non-essential gene encoding a g-protein-coupled chemokine receptor subfamily homologue (gpcr) was targeted as an additional insertion site. peste des petits ruminants (ppr) was chosen as a disease model. a new ...201728576653
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