| plant regeneration through somatic embryogenesis in callus culture of green bamboo (bambusa oldhamii munro). | young inflorescence explants of green bamboo (bambusa oldhamii munro) in culture show a high capacity for plant regeneration through somatic embryogenesis. embryogenic callus was initiated from explants maintained on murashige and skoog's medium supplemented with 3 mg/l 2,4-d, 2 mg/l kinetin and a high content (60 g/l) of sucrose. prolonged culture in the embryoid induction medium or transferral of embryonic callus to auxin-free medium resulted in the continued development and eventual germinati ... | 1986 | 24240845 |
| molecular cloning and functional identification of invertase isozymes from green bamboo bambusa oldhamii. | three bo beta fruct cdnas encoding acid invertases were cloned from shoots of the green bamboo bambusa oldhamii. on the basis of the amino acid sequences of their products and phylogenetic analyses, bo beta fruct1 and bo beta fruct2 were determined to encode cell wall invertases, whereas bo beta fruct3encodes a vacuolar invertase. the recombinant proteins encoded by bo beta fruct2 and bo beta fruct3 were produced in pichia pastoris and purified to near homogeneity using ammonium sulfate fraction ... | 2006 | 16608237 |
| complete nucleotide sequence of dendrocalamus latiflorus and bambusa oldhamii chloroplast genomes. | although bamboo is one of the most important woody crops in asia, information on its genome is still very limited. to investigate the relationship among poaceae members and to understand the mechanism of albino mutant generation in vitro, the complete chloroplast genome of two economically important bamboo species, dendrocalamus latiflorus munro and bambusa oldhamii munro, was determined employing a strategy that involved polymerase chain reaction (pcr) amplification using 443 novel primers desi ... | 2009 | 19324693 |
| retraction: cloning, expression and characterization of udp-glucose pyrophosphorylase from shoots of bambusa oldhamii. | the paper has been published online in journal of biochemistry advance access and had been submitted without agreement from the co-authors. they therefore retract this paper and discourage citations of it. | 2009 | 19505952 |
| mining of ssr markers from expressed sequence tags of bamboo species. | with the ever increasing number of expressed sequence tags (ests) from various sequencing projects, ests have become valuable and first-hand source of in-silico mining of simple sequence repeats (ssr) markers. we examined a total of 3419 est sequences from three bamboo species, namely, phyllostachys edulis, bambusa oldhamii and dendrocalamus sinicus for the presence of di- to hexa- microsatellites. the frequency of ssr containing ests varied from 5.36% in b. oldhamii to 13.05% in p. edulis. no s ... | 2010 | 21364824 |
| molecular characterization of a phenylalanine ammonia-lyase gene (bopal1) from bambusa oldhamii. | phenylalanine ammonia-lyase is the first enzyme of general phenylpropanoid pathway. a pal gene, designated as bopal1, was cloned from a bambusa oldhamii cdna library. the open reading frame of bopal1 was 2,139 bp in size and predicted to encode a 712-amino acid polypeptide. bopal1 was the first intronless pal gene found in angiosperm plant. several putative cis-acting elements such as p box, gt-1motif, and solips involved in light responsiveness were found in the 5'-flanking sequence of bopal1 w ... | 2011 | 20354908 |
| comparative study of the resistance of six hawaii-grown bamboo species to attack by the subterranean termites coptotermes formosanus shiraki and coptotermes gestroi (wasmann) (blattodea: rhinotermitidae). | bamboo is widely grown and utilized as a construction material around the world, particularly in the tropics. at present, there are about 70 bamboo species and varieties recorded from hawaii. the objective of our study was to determine the relative resistance of six hawaii-grown bamboo species to attack by coptotermes formosanus shiraki and coptotermes gestroi (wasmann). four-week laboratory feeding trials were performed as described in standard e1-09 of the american wood protection association ... | 2011 | 26467827 |
| mitochondrial energy metabolism in young bamboo rhizomes from bambusa oldhamii and phyllostachys edulis during shooting stage. | the energy metabolism of mitochondria in young rhizomes of the bamboo species bambusa oldhamii, which favors shooting during the summer, and phyllostachys edulis, which favors shooting during the winter, was characterized. the mitochondrial energy-converting system was clarified in terms of respiratory activity and structural organization. the respiration rates were measured at 15, 28, and 42 °c by nadh, succinate, and malate oxidation. nadh was shown to act as an efficient substrate regardless ... | 2011 | 21334908 |
| analysis of the expression of bohlol1, which encodes an lsd1-like zinc finger protein in bambusa oldhamii. | a cdna, bohlol1, encoding a protein containing three zf-lsd1 (zinc finger-lesions simulating disease resistance 1) domains was cloned from growing bamboo (bambusa oldhamii) shoots. a phylogenetic analysis revealed that bohlol1 is a homolog of arabidopsis lsd1 and lol1 (lsd-one-like 1), which have been reported to act antagonistically in controlling cell death via the maintenance of reactive oxygen species homeostasis. the bohlol1 gene was differentially expressed in various bamboo shoot tissues ... | 2011 | 21744093 |
| identification of genes differentially expressed during the growth of bambusa oldhamii. | bamboos are ecologically and economically important grasses, and are distinguished by their rapid growth. to identify genes associated with bamboo growth, pcr-based mrna differential display was used to clone genes that were differentially expressed in various tissues of bamboo (bambusa oldhamii) shoots at different growth stages. in total, 260 different cdna sequences were obtained. these genes displayed complex expression profiles across the different tissues and growth stages as revealed by a ... | 2013 | 23291655 |
| understanding bamboo flowering based on large-scale analysis of expressed sequence tags. | unlike other plants, bamboo (bambusoideae) flowering is an elusive physiological phenomena, because it is unpredictable, long-periodic, gregarious, and uncontrollable; also, bamboo plants usually die after flowering. the flowering mechanism in arabidopsis thaliana, a eudicot model species, is well established, but it remains unknown in bamboo species. we found 4470 and 3878 expressed sequence tags in the flower bud and vegetative shoot cdna libraries, respectively, of the bamboo species, bambusa ... | 2010 | 20568053 |
| ectopic expression of the botfl1-like gene of bambusa oldhamii delays blossoming in arabidopsis thaliana and rescues the tfl1 mutant phenotype. | terminal flower1 (tfl1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo tfl1 homologous gene has not been described. in this study, a tfl1 homologous gene was isolated from bambusa oldhamii and designated as botfl1-like. phylogenetic analysis of tfl1 homologous genes revealed that botfl1-like shared more than 90% identity with the tfl1 genes of other gramineae. rt- ... | 2015 | 26345864 |
| functional analysis of pi-like gene in relation to flower development from bamboo (bambusa oldhamii). | bamboo flowering owns many unique characteristics and remains a mystery. to investigate the molecular mechanisms underlying flower development in bamboo, a petal-identity gene was identified as a pistillata homologue named bopi from bambusa oldhamii (bamboo family). expression analysis showed that bopi was highly expressed in flower organs and gradually increased during flower development stage, suggesting that bopi played an important role in flower development. ectopic expression of bopi in ar ... | 2016 | 27019434 |
| cloning and expression of a phenylalanine ammonia-lyase gene (bopal2) from bambusa oldhamii in escherichia coli and pichia pastoris. | phenylalanine ammonia-lyase (pal, ec 4.3.1.5) is the first committed enzyme of phenylpropanoid pathway. a pal gene, designated as bopal2, was cloned from a bambusa oldhamii cdna library. the open reading frame of bopal2 was 2142bp in size encoding a 713-amino acid polypeptide. bopal2 was heterologous expressed in escherichia coli and pichia pastoris. the recombinant proteins were exhibited pal and tyrosine ammonia-lyase activities. the recombinant bopal2 had a subunit mass of 80kda and existed a ... | 2010 | 20064614 |
| subcellular localization and expression of bamboo mosaic virus satellite rna-encoded protein. | the satellite rna of bamboo mosaic virus (satbamv) has a single open reading frame encoding a non-structural protein, p20, which facilitates long-distance movement of satbamv in bamv and satbamv co-infected plants. immunohistochemistry and immunoelectron microscopy revealed that the p20 protein accumulated in the cytoplasm and nuclei in co-infected cells. p20 and the helper virus coat protein (cp) were highly similar in their subcellular localization, except that aggregates of bamv virions were ... | 2009 | 19141462 |
| cloning and characterization of an antifungal class iii chitinase from suspension-cultured bamboo ( bambusa oldhamii ) cells. | a class iii chitinase cdna (bochi3-1) was cloned using a cdna library from suspension-cultured bamboo ( bambusa oldhamii ) cells and then transformed into yeast ( pichia pastoris x-33) for expression. two recombinant chitinases with molecular masses of 28.3 and 35.7 kda, respectively, were purified from the yeast's culture broth to electrophoretic homogeneity using sequential ammonium sulfate fractionation, phenyl-sepharose hydrophobic interaction chromatography, and con a-sepharose chromatograp ... | 2008 | 18998701 |
| molecular characterization and expression of four cdnas encoding sucrose synthase from green bamboo bambusa oldhamii. | bamboo is distinguished by its rapid growth. to investigate sucrose metabolism in this plant, we cloned the cdnas encoding sucrose synthase (sus) from bambusa oldhamii and investigated their expression in growing shoots and leaves. four cdna clones, bosus1, bosus2, bosus3 and bosus4, were isolated by screening a cdna library from etiolated bamboo shoots. recombinant bosus proteins were produced in escherichia coli and purified by immobilized metal affinity chromatography and ultrafiltration. sem ... | 2006 | 16539603 |
| analysis of the cellulose synthase genes associated with primary cell wall synthesis in bambusa oldhamii. | the synthesis of cell wall polysaccharides is highly active in rapidly growing bamboo shoots. we cloned a set of bocesa cdnas that encode cellulose synthase from bamboo (bambusa oldhamii) and investigated the expression patterns of the bocesa2, bocesa5, bocesa6 and bocesa7 genes. the four bocesa genes were differentially expressed in the different parts of growing bamboo shoots, in various organs, and in multiple shoots that were cultured in vitro. they were down-regulated by alpha-naphthaleneac ... | 2010 | 20541781 |
| characterization of copper/zinc and manganese superoxide dismutase in green bamboo (bambusa oldhamii): cloning, expression and regulation. | bamboo is distinguished by its rapid growth, for growth more than 100 cm per day. because of the rapid growth, tissues have significant atp requirements, which results in intense reduction of oxygen and thus oxidative stress. for this reason, bamboo may have a special and efficient scavenger system to release the stress during fast cell division and elongation. here, we investigated superoxide dismutase (sod, e.c.1.15.1.1), the first line of antioxidant enzymes, in green bamboo (bambusa oldhamii ... | 2011 | 21169026 |
| differential expression of genes encoding acid invertases in multiple shoots of bamboo in response to various phytohormones and environmental factors. | the promoter regions of two cell wall invertase genes, boβfruct1 and boβfruct2, and a vacuolar invertase gene, boβfruct3, in bambusa oldhamii were cloned, and putative regulatory cis-elements were identified. the expression of these three genes in multiple shoots of bamboo that were cultured in vitro under different conditions was analyzed by real-time pcr. the two cell wall invertase genes were upregulated by indole-3-acetic acid and cytokinins but responded differently to other phytohormones a ... | 2013 | 23586540 |
| effects of high nutrient supply on the growth of seven bamboo species. | over the last decade, bamboo has emerged as an interesting plant for the treatment of various polluted waters using plant-based wastewater treatment systems. in these systems, nitrogen and phosphorous concentrations in wastewater can exceed plant requirements and potentially limit plant growth. the effects of two nutrient rates on the growth of seven bamboo species were assessed in a one-year experiment: dendrocalamus strictus, thyrsostachys siamensis, bambusa tuldoides, gigantochloa wrayi, bamb ... | 2014 | 24933901 |
| cloning, expression, site-directed mutagenesis and immunolocalization of phenylalanine ammonia-lyase in bambusa oldhamii. | phenylalanine ammonia-lyase (pal, ec 4.3.1.5) from green bamboo was isolated and cloned from the shell of bambusa oldhamii. the k(m) of bamboo shell pal for l-phe was 476 μm, and the molecular mass of native pal was estimated as 275 kda and the molecular mass of a subunit was about 76 kda, indicating that pal from bamboo also exists as a tetramer. the optimum temperature for pal activity was 50°c and the optimal ph 9.0. the identity of the purified bamboo shell pal was confirmed using q-tof tand ... | 2010 | 21035152 |