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vitek 2 automated identification system and kocuria kristinae. 200516272536
volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus).chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ...200521132979
a biotechnological process for treatment and recycling poultry feathers as a feed ingredient.a strain of kocuria rosea with keratinolytic capacity was cultured aerobically on submerged feathers to obtain a fermented feather meal (ffm). this ffm enriched with cells of k. rosea mainly contains crude protein (71%). the pepsin digestibility of the fermented product (88%) was similar to the value of the commercial feather meal and more than 70% greater that untreated feathers. the bacterial biomass improved the content of amino acids lysine (3.46%), histidine (0.94%) and methionine (0.69%). ...200516023573
identification and differentiation of staphylococcus carnosus and staphylococcus simulans by species-specific pcr assays of soda genes.the aim of this study was to design species-specific pcr assays for rapid and reliable identification and differentiation of staphylococcus (s.) carnosus and s. simulans strains. two different sets of primers, targeting the manganese-dependent superoxide dismutase (soda) gene of s. carnosus and s. simulans, respectively, were designed. species-specificity of both sets of primers was evaluated by using 93 strains, representing 26 different species of the genus staphylococcus, 3 species of the gen ...200516106559
molecular survey of aeroplane bacterial contamination.to examine bacterial contamination of passenger aircraft and to identify aeroplane environments posing the greatest potential health risk.200516108791
kocuria kristinae infection associated with acute cholecystitis.kocuria, previously classified into the genus of micrococcus, is commonly found on human skin. two species, k. rosea and k. kristinae, are etiologically associated with catheter-related bacteremia.200516029488
reclassification of strain ccm 132, previously classified as kocuria varians, as kocuria carniphila sp. nov.a gram-positive actinobacterium, previously classified as kocuria varians, was subjected to a polyphasic taxonomic study. the bacterium showed the peptidoglycan type lys-ala3 (variation a3alpha), mk-7(h2) was the major menaquinone and anteiso-c(15 : 0) and anteiso-c(17 : 0) were the major fatty acids. on the basis of the phylogenetic and phenotypic characteristics of the actinobacterium, a novel species, kocuria carniphila sp. nov. (type strain, ccm 132t=dsm 16004t), is proposed.200515653866
erroneous reporting of coagulase-negative staphylococci as kocuria spp. by the vitek 2 system.misidentification of coagulase-negative staphylococci (cons) may delay appropriate treatment. we investigated 20 clinical isolates identified as kocuria spp. by the vitek 2 system. all were identified as cons by 16s rrna gene sequencing (18 staphylococcus epidermidis, 1 staphylococcus haemolyticus). four kocuria isolates were shown to be identical to cons from the same patient by pulsed-field gel electrophoresis. isolates identified by vitek 2 as kocuria most likely represent misidentified cons, ...200515750130
comparison of various assays used for detection of beta-lactam antibiotics in poultry meat.in this study, microbiological tests for the detection of beta-lactam antibiotics in meat and meat products were evaluated. the traditional fpt (four plate test, containing bacillus subtilis and kocuria rhizophila), bsda (bacillus stearothermophilus disc assay) and a newly developed microbiological test, premi test (containing bacillus stearothermophilus) were included in the study. the limit of detection (lod) of the premi test was compared with the lod of the traditional methods. the detection ...200516019829
[gram-positive cocci as an opportunistic infection factor].the frequency of opportunistic infections increased in the last years. the opportunistic infections are serious and very difficult to diagnose complication at patients with lower immunity. the following factors are conducive to develop infections caused by opportunistic bacteria: invasive research methods, medical treatments and immunological defects. the publication presents pathogenicity of bacteria of following types: aerococcus, kocuria, kytococcus, lactococcus, leuconostoc, micrococcus, ped ...200516161935
use of field-based stable isotope probing to identify adapted populations and track carbon flow through a phenol-degrading soil microbial community.the goal of this field study was to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol. our approach measured 13co2 respired from [13c]phenol and stable isotope probing (sip) of soil dna at an agricultural field site. traditionally, sip-based investigations have been subject to the uncertainties posed by carbon cross-feeding. by altering our field-based, substrate-dosing methodologies, experiments were designed to look beyond primary degrad ...200516332760
validation of a microbiological method: the star protocol, a five-plate test, for the screening of antibiotic residues in milk.the results of an in-house laboratory validation of a microbiological method for the screening of antibiotic residues in milk are presented. the sensitivity of this five-plate test, called screening test for antibiotic residues (star), was established by the analysis of milk samples spiked with 66 antibiotics at eight different concentrations. ten different groups of antibiotics were studied: macrolides, aminoglycosides, cephalosporins, penicillins, quinolones, tetracyclines, sulphonamides, linc ...200415204543
kocuria marina sp. nov., a novel actinobacterium isolated from marine sediment.an actinobacterial strain was isolated from marine sediment taken from the troitsa bay of the gulf of peter the great, east siberian sea, and subjected to a taxonomic investigation. the isolate, designated kmm 3905t, was gram-positive, aerobic, non-motile and coccoid. the strain tolerated up to 15 % nacl in growth media, although its presence was not essential for growth. chemotaxonomic and phylogenetic properties of the strain were consistent with its classification in the genus kocuria. the st ...200415388718
contribution of starter cultures to the proteolytic process of a fermented non-dried whole muscle ham product.porcine longissimus dorsi muscles were cured by brine injection. curing brine containing 15% (w/v) nacl, 1.33% (w/v) glucose, 750 ppm sodium nitrite, and appropriate levels of either lactobacillus sakei lad, l. sakei lad plus kocuria varians ft4 (formally micrococcus varians), l. sakei lad plus papain and gdl (glucono-delta-lactone) plus k. varians ft4, was injected to the muscle at a pumping rate 15% w/v. the effect of these treatments on the proteolysis in the ham system was compared to a cont ...200415135960
catheter-related bacteremia due to kocuria rosea in a patient undergoing peripheral blood stem cell transplantation.micrococcus species may cause intracranial abscesses, meningitis, pneumonia, and septic arthritis in immunosuppressed or immunocompetent hosts. in addition, strains identified as micrococcus spp. have been reported recently in infections associated with indwelling intravenous lines, continuous ambulatory peritoneal dialysis fluids, ventricular shunts and prosthetic valves.200415615593
meat-model system development for proteolytic activity determination.many technological parameters that affect the nature and functional properties of proteins are involved in the preparation of meat products. the dry-curing process is quite complex because of the coexistence of enzymes from endogenous and bacterial origins. the protein breakdown that takes place during the ripening of dry fermented sausages leads to an increase in the concentration of peptides and free amino acids. the proteolytic events have been thoroughly investigated not only because of thei ...200415156060
antimicrobial potential of immobilized lactococcus lactis subsp. lactis atcc 11454 against selected bacteria.immobilization of living cells of lactic acid bacteria could be an alternative or complementary method of immobilizing organic acids and bacteriocins and inhibit undesirable bacteria in foods. this study evaluated the inhibition potential of immobilized lactococcus lactis subsp. lactis atcc 11454 on selected bacteria by a modified method of the agar spot test. l. lactis was immobilized in calcium alginate (1 to 2%)-whey protein concentrate (0 and 1%) beads. the antimicrobial potential of immobil ...200415222547
development of a multiplex pcr for the identification of staphylococcus genus and four staphylococcal species isolated from food.to develop a multiplex pcr that allows the identification of bacteria belonging to the staphylococcus genus and in particular to the species staphylococcus xylosus, s. saprophyticus, s. epidermidis and s. aureus isolated from food manufacturing plants.200415479426
interactions in biofilms between listeria monocytogenes and resident microorganisms from food industry premises.twenty nine bacterial strains were grown as binary culture biofilms with listeria monocytogenes to assess their influence on the settlement of the latter on stainless steel coupons. most of the strains had been isolated from food processing plants after cleaning and disinfection and were tentatively identified by the apilab plus 3.3.3 database (biomerieux). sixteen of them decreased l. monocytogenes biofilm colony forming units (cfu) counts. three strains, bacillus sp. ccl 9 an unidentified gram ...200415541798
bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations.heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ...200415574939
rapid and reliable identification of staphylococcus equorum by a species-specific pcr assay targeting the soda gene.rapid and reliable identification of staphylococcus (s.) equorum was achieved by species-specific pcr assays. a set of primers targeting the manganese-dependent superoxide dismutase (soda) gene of s. equorum was designed. species-specificity of the primer set was evaluated by using a total of 112 strains (including 27 reference strains of the dsm collection), representing 26 different species of the genus staphylococcus, 3 species of the genus kocuria, and different strains of macrococcus caseol ...200415612627
isolation and technological properties of coagulase negative staphylococci from fermented sausages of southern italy.the aims of this study were to characterize the population of micrococcaceae in different types of fermented sausages of southern italy and to determine the technological properties of staphylococcus strains in order to evaluate the suitability of selected strains as starter cultures in the processing of dry fermented pork sausages. ninety-six strains were studied to evaluate nitrate reductase, proteolytic, lipolytic and antioxidant activities as well as growth ability at different temperatures, ...200422061128
[occurrence and number of bacteria from the micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus genera on skin and mucous membranes in humans].the aim of work was to evaluate the frequency of occurrence of bacteria from genera of micrococcus, kocuria, nesterenkonia, kytococcus and dermatococcus on human skin and mucous membranes in healthy population. among 150 investigated persons these bacteria were found in 122 (81.3%). the frequency of isolation was similar in both sex (82.4% in female and 79.7% in male). most often the strains were isolated from oral cavity (48.7%), and from skin of palm and forearm (40.7% and 37.3%). least freque ...200312908417
infection of a ventriculoatrial shunt with phenotypically variable staphylococcus epidermidis masquerading as polymicrobial bacteremia due to various coagulase-negative staphylococci and kocuria varians.the diagnosis of bloodstream infection with coagulase-negative staphylococci is frequently based on the isolation of the same organism from more than one blood culture. phenotypic variation is a common characteristic of pathogenic strains of staphylococcus epidermidis which may affect species identification by the microbiology laboratory. we describe a patient with a new onset of nephritis and gram-positive bacteremia. gram-positive cocci grew in multiple blood cultures and were identified by th ...200312791862
ecology of mixed biofilms subjected daily to a chlorinated alkaline solution: spatial distribution of bacterial species suggests a protective effect of one species to another.three bacterial strains (kocuria sp. c714.1, brevibacterium linens b337.1 and staphylococcus sciuri ccl101) were grown together on stainless steel and were subjected daily to a commercial alkaline chlorine solution (22 mg l-1 of free chlorine, ph 11) over a period of 4 weeks. after the daily chemical shock, culture madia [1:20 dilution of tryptic soy broth (tsb-ye/20) or diluted whey] was deposited on the biofilms. the chemical shocks led first to a drop in the culturable population, followed by ...200312542714
[susceptibility to antibiotics of bacteria from genera micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus].two hundred and nineteen strains from genera micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus isolated from different sources, such as saliva, skin of palm and forearm and from vestibule of nose were tested. the susceptibility to doxycycline, cetriaxone, cefuroxyme, amikacyne, amoksycillin with clavulanic acid, trimethoprim/sulfamethoxazole, ampicillin, ciprofloxacin, penicillin and erythromycin were estimated. in general, bacteria from these genera are sensitive to most of select ...200312908418
[production of oil-processing compounds by microorganisms from the daqing oil field, china].twenty pure cultures isolated from formation waters of the daqing oil field were studied with respect to their capacity to produce surface-active compounds in media with individual hydrocarbons, lower alcohols, and fatty acids. aerobic saprotrophic bacteria belonging to the genera bacillus, brevibacillus, rhodococcus, dietzia, kocuria, gordonia, cellulomonas, clavibacter, pseudomonas, and acinetobacter decreased the surface tension of cultivation media from 55-63 to 28-44 mn/m. strains of bacill ...200312751245
combining denaturing gradient gel electrophoresis of 16s rdna v3 region and 16s-23s rdna spacer region polymorphism analyses for the identification of staphylococci from italian fermented sausages.separation of amplified v3 region from 16s rdna by denaturing gradient gel electrophoresis (pcr-dgge) and 16s-23s rdna intergenic spacer region polymorphism (isr-pcr) analyses were tested as tool for differentiation of staphylococcal strains commonly isolated from fermented sausages. variable v3 regions of 25 staphylococcal reference strains and 96 wild strains of species belonging to the genera staphylococcus, micrococcus and kocuria were analyzed. pcr-dgge profiles obtained were species-specif ...200314529185
design and evaluation of specific pcr primers for rapid and reliable identification of staphylococcus xylosus strains isolated from dry fermented sausages.rapid and reliable identification of staphylococcus xylosus was achieved by species-specific pcr assays. two sets of primers, targeting on xylulokinase (xylb) and 60 kda heat-shock protein (hsp60) genes of s. xylosus, respectively, were designed. species-specificity of both sets of primers was evaluated by using 27 reference strains of the dsm collection, representing 23 different species of the staphylococcus genus and 3 species of the kocuria genus. moreover, 90 wild strains isolated from diff ...200314666989
a survey of antibiotic resistance in micrococcaceae isolated from italian dry fermented sausages.the transfer of bacteria that are resistant to antimicrobial agents or resistance genes from animals to humans via the food chain is increasingly a problem. therefore, it is important to determine the species and the numbers of bacteria involved in this phenomenon. for this purpose, 148 strains of microstaphylococci were isolated from three types of italian dry fermented sausages. eight of 148 strains belonged to the genera kocuria and micrococcus. the remaining 140 strains belonged to 11 differ ...200312800992
kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.strain cms 76ort, an orange-pigmented bacterium, was isolated from a cyanobacterial mat sample from a pond located in mcmurdo dry valley, antarctica. on the basis of chemotaxonomic and phylogenetic properties, strain cms 76ort was identified as a member of the genus kocuria. it exhibited a 16s rdna similarity of 99.8% and dna-dna similarity of 71% with kocuria rosea (atcc 186t). phenotypic traits confirmed that strain cms 78ort and k. rosea were well differentiated. furthermore, strain cms 76ort ...200312656171
time series analysis of aerobic bacterial flora during miso fermentation.this article reports a microbiological study of aerobic mesophilic bacteria that are present during the fermentation process of miso.200312859661
reclassification of atcc 9341 from micrococcus luteus to kocuria rhizophila.strain atcc 9341, currently known as micrococcus luteus, has been designated as a quality-control strain in a number of applications. it is also cited as the standard culture in several official methods and manuals, as well as the code of federal regulations. over the years, it has become apparent that atcc 9341 does not resemble other m. luteus strains; however, its phenotypic characteristics alone were ambiguous. recently, a polyphasic study was performed in which molecular data were combined ...200312892116
bacterial and fungal aerosols in indoor environment in central and eastern european countries.studies of indoor bioaerosols conducted in central and eastern european countries, as a result of the scarcity of funding, mostly do not attain the level presented by similar studies in northern america and western europe. for socio-economic reasons, most of the intense studies on indoor bioaerosols in central and eastern european countries were carried out in industrial facilities and have contributed significantly to occupational health science. in contrast, until recently, insufficient of stu ...200212088392
prevalent bacterial species and novel phylotypes in advanced noma lesions.the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ...200212037085
aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis angusticollis.to demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis.200211849325
catheter-related bacteremia due to kocuria kristinae in a patient with ovarian cancer.we report on the first case of a catheter-related recurrent bacteremia caused by kocuria kristinae, a gram-positive microorganism belonging to the family micrococcaceae, in a 51-year-old woman with ovarian cancer. this unusual pathogen may cause opportunistic infections in patients with severe underlying diseases.200211773142
[occurrence of bacteria in the mouth from genera of micrococcus, kocuria, nesterenkonia, kytococcus and dermacoccus].the aim of the study was to assess the prevalence of different bacteria in the oral cavity. the bacteria were present in the oral cavities of 73 (48.7%) of 150 individuals. nesterenkonia halobia, the most frequently isolated species, was found in 20 (27%) individuals, micrococcus luteus in 16 (22%), kocuria kristinae in 12 (16%), kocuria varians in 10 (14%), dermacoccus sedentarius in 9 (12%), micrococcus lylae in 8 (11%), and kytococcus nishinomiyaensis in 3 (4%). mean counts of these microorga ...200212185681
[phylogenetic diversity of aerobic saprotrophic bacteria isolated from the daqing oil field].a diverse and active microbial community in the stratal waters of the daqing oil field (china), which is exploited with the use of water-flooding, was found to contain aerobic chemoheterotrophic bacteria (including hydrocarbon-oxidizing ones) and anaerobic fermentative, sulfate-reducing, and methanogenic bacteria. the aerobic bacteria were most abundant in the near-bottom zones of injection wells. twenty pure cultures of aerobic saprotrophic bacteria were isolated from the stratal waters. under ...200211910798
gamma irradiation and cellular damage in kocuria rosea: investigation by one- and two-dimensional infrared spectroscopy.fourier transform infrared (ft-ir) spectroscopy was used to investigate the radiation-induced effects on kocuria rosea. bacterial suspensions at the stationary phase were exposed to increasing doses of gamma radiation. in the region 1350-840cm(-1), assigned to phosphodiester backbone, nucleic acids, and sugar rings, the radical damaging effects were dose-dependent, with the first threshold at 2.75kgy and the second at 13.75kgy inducing more striking spectral variations. postirradiation reincubat ...200212464274
analysis of biogenic amines in northern and southern european sausages and role of flora in amine production.the biogenic amine contents, microbial counts and flora producing amines were investigated in four types of fermented sausages. southern type european sausages (italian and belgian) showed higher tyramine and phenylethylamine values than northern type ones (norwegian and belgian). the spontaneous non-starter lactic acid bacteria could be responsible for the production of these amines in the italian products, and the cocci gram positive in the belgian south ones. the norwegian sausages showed the ...200222064002
identification by 16s-23s rdna intergenic region amplification, genotypic and phenotypic clustering of staphylococcus xylosus strains from dry sausages.to ascertain the identification and typing of the gram-positive, coagulase-negative cocci present in 'salsiccia sotto sugna', an italian artisanal sausage.200111298231
the application of a fermented food ingredient containing 'variacin', a novel antimicrobial produced by kocuria varians, to control the growth of bacillus cereus in chilled dairy products.the feasibility of applying variacin, a lantibiotic produced by kocuria varians in the form of a spray-dried fermented ingredient to control the growth of psychrotrophic bacillus cereus strains in chilled dairy foods, was evaluated.200111155129
potential use of presumptive enterococci and staphylococci as indicators of sanitary condition in plants making hard italian-type cheese.raw milk, pasteurized milk, unripened cheese (1 day old), and partially ripened cheese (3 months) from 42 milk lots at a plant making hard italian-type cheese were analyzed for presumptive enterococci using kanamycin esculin azide agar pour plates. fully ripened (> or =10 months) cheeses, derived from other milk lots, were also tested. numbers of presumptive staphylococci (baird-parker agar [b-p]) were determined in the partially and fully ripened cheeses. presumptive enterococci were ubiquitous ...200011131893
[unique properties of highly radioresistant bacteria].in connection with the chernobyl nuclear power plant (chnpp) accident and the negative ecological after-effects for biota in this zone the interest has arisen to radioresistant bacteria, as to the most dynamic model of the given ecosystem, and to mechanisms which provide resistance of bacteria to ionizing radiation. the analysis of published data has shown that the radioresistant bacteria are not interrelated taxonomically and phylogenetically. the extreme radioresistant bacteria are represented ...200011300085
characterization of micrococcaceae isolated from salt used for spanish dry-cured ham.the microbial flora of salt used in the production of spanish dry-cured ham was studied. the results indicated that micrococcaceae constituted the predominant flora. identification of the 369 isolates belonging to the family micrococcaceae revealed that 60% belonged to genus staphylococcus, 25% to micrococcus, 6% to kocuria, 5.4% to dermacoccus and 0.5% to stomatococcus. the species most often isolated was staph. xylosus (28.9%), followed by m. lylae (21.4%), staph. equorum (18.55%) and d. nishi ...200011068912
the status of the genus pelczaria (poston 1994) and the species pelczaria aurantia (poston 1994). request for an opinion.based upon the results of another publication [p. schumann et al. (2000). int j syst evol microbiol 50, 1421-1424) it is concluded that the culture pelczaria aurantia atcc 49321t (= dsm 12801t) currently being distributed does not conform to the description of the type strain of pelczaria aurantia (poston 1994) and the type species of the genus pelczaria (poston 1994). it is proposed that the judicial commission consider (1) that the organism currently deposited as atcc 49321t and dsm 12801t be ...200010939680
pelczaria aurantia atcc 49321t (=dsm 12801t) is a strain of kocuria rosea (flügge 1886) stackebrandt et al. 1995.phylogenetic and chemotaxonomic analyses of pelczaria aurantia atcc 49321t (= dsm 12801t) indicate that this species is very closely related to kocuria rosea. the dna-dna reassociation value of 87.1% determined for the type strains of the two species supports this finding. the results of phylogenetic analysis of the 16s rdna of a subculture of the original strain of pelczaria aurantia, deposited at the national institutes of health, bethesda, md, usa, as 'neisseria aurantia', are identical to th ...200010939645
phylogenetic and physiological diversity of arthrobacter strains isolated from unconsolidated subsurface sediments.forty strains of gram-positive, aerobic, heterotrophic bacteria isolated from saturated subsurface lacustrine, paleosol and fluvial sediments at the us department of energy's hanford site in south central washington state were characterized by phylogenetic analysis of 16s rrna gene sequences and by determination of selected morphological, physiological and biochemical traits. phylogenetic analyses of 16s rdna sequences from subsurface isolates in the context of similar sequences from previously ...200010846209
[the use of batumin-containing disks for the rapid identification of staphylococci].the study of 467 microbial strains obtained from collections and from clinical sources revealed that microorganisms of the genus staphylococcus were highly sensitive to batumin, a new antibiotic obtained from bacteria of the genus pseudomonas. 378 strains of 15 staphylococcus species proved to be highly sensitive to the diagnostic preparation "diastaph", developed on the basis of batumin (antibiotic-impregnated discs); after 18-hour incubation the diameter of the growth inhibition zones on agar- ...199910852028
size distribution of bacterial and fungal bioaerosols in indoor air.the aim of this study was to determine the size distribution of bacteria and fungi occurring in the air of human dwellings. the concentration and size distribution of particulate aerosol, gram-positive mesophilic bacteria, gram-negative mesophilic bacteria and fungi were examined in 60 flats situated in the upper silesia conurbation, southern poland. the investigated flats comprised three quantitatively equal (20 flats each) groups: flats without additional emission sources of particulate aeroso ...199910607991
a 23s rdna-targeted polymerase chain reaction-based system for detection of staphylococcus aureus in meat starter cultures and dairy products.a polymerase chain reaction-based system for detection of staphylococcus aureus was developed. the system consisted of the following components: (i) selective enrichment, (ii) dna isolation, (iii) amplification of dna with primers targeted against the 23s rrna gene, and (iv) evaluation of the specificity of the polymerase chain reaction by southern hybridization and nested polymerase chain reaction. the method achieved a high degree of sensitivity and unambiguity as required for the detection of ...199910528718
kocuria palustris sp. nov. and kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (typha angustifolia).two gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (typha angustifolia) collected from a floating mat in the soroksár tributary of the danube river, hungary. sequence comparisons of the 16s rdna indicated these isolates to be phylogenetic neighbours of members of the genus kocuria, family micrococcaceae, in which they represent two novel lineages. the phylogenetic distinctness of the two organisms ta68t and taga27t was supported by dna- ...199910028258
phylogenetic diversity of the deinococci as determined by 16s ribosomal dna sequence comparison.16s ribosomal dna (rdna) sequences were determined for the five species of the genus deinococcus (deinococcus erythromyxa, deinococcus proteolyticus, deinococcus radiodurans, deinococcus radiophilus, and deinococcus radiopugnans) and the single species of the genus. deinobacter (deinobacter grandis). with the exception of deinococcus erythromyxa, the deinococci form a coherent phylogenetic cluster which is related to the thermus-meiothermus lineage. an analysis of the 16s rdna sequence of deinoc ...19979103641
taxonomic dissection of the genus micrococcus: kocuria gen. nov., nesterenkonia gen. nov., kytococcus gen. nov., dermacoccus gen. nov., and micrococcus cohn 1872 gen. emend.the results of a phylogenetic and chemotaxonomic analysis of the genus micrococcus indicated that it is significantly heterogeneous. except for micrococcus lylae, no species groups phylogenetically with the type species of the genus, micrococcus luteus. the other members of the genus form three separate phylogenetic lines which on the basis of chemotaxonomic properties can be assigned to four genera. these genera are the genus kocuria gen. nov. for micrococcus roseus, micrococcus varians, and mi ...19957547287
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