| kloeckera apis st. nov.; the imperfect state of hanseniaspora guilliermondii pijper. | k. apiculata var. apis (nom. nud.) was found to be the imperfect state of h. guilliermondii pijper by a high degree of dna reassociation. the name is validated and raised to species rank, k. apis lavie ex smith, simione and meyer. k. apis and h. guilliermondii could be distinguished from h. uvarum and h. valbyensis by a low dna reassociation and by growth at 37 c. | 1977 | 596864 |
| ultrastructure of hanseniaspora ascospores. | a comparative study of the ultrastructure in sections of the ascospores of six hanseniaspora species showed three types of spores: (1) hat-shaped in h. valbyensis and h. guilliermondii, (2) spherical with an equatorial or subequatorial ledge, smooth or rough in h. occidentalis and h. uvarum, (3) spherical with warts in h. osmophila and h. vineae. development and germination of the spores of hanseniaspora guilliermondii is described in more detail. | 1977 | 596865 |
| biometric study of acetoin production in hanseniaspora guilliermondii and kloeckera apiculata. | gas chromatographic analysis by direct injection of samples yielded quantitative data on acetoin content. ninety-six strains of hanseniaspora guilliermondii and kloeckera apiculata were investigated for the ability to produce acetoin in synthetic medium and in must. high-level production of acetoin was found to be a characteristic of both species. in synthetic medium, the two species were not significantly different with respect to sugar utilization and ethanol or acetoin production. in grape mu ... | 1993 | 16348961 |
| rational selection of non-saccharomyces wine yeasts for mixed starters based on ester formation and enological traits. | thirty-eight yeast strains belonging to the genera candida, hanseniaspora, pichia, torulaspora and zygosaccharomyces were screened for ester formation on synthetic microbiological medium. the genera hanseniaspora and pichia stood out as the best acetate ester producers. based on the ester profile hanseniaspora guilliermondii 11027 and 11102, hanseniaspora osmophila 1471 and pichia membranifaciens 10113 and 10550 were selected for further characterization of enological traits. when growing on mus ... | 2008 | 18620969 |
| the microbiota of lafun, an african traditional cassava food product. | lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ... | 2009 | 19493582 |
| heavy sulphur compounds, higher alcohols and esters production profile of hanseniaspora uvarum and hanseniaspora guilliermondii grown as pure and mixed cultures in grape must. | hanseniaspora guilliermondii and hanseniaspora uvarum were tested in grape must fermentations as pure and mixed starter cultures with saccharomyces cerevisiae. in pure cultures, the specific growth rates found were 0.29 h(-1) for h. uvarum, 0.23 h(-1) for h. guilliermondii and 0.18 h(-1) for s. cerevisiae. no significant differences were observed between these values and those obtained in mixed cultures. results presented in this work show that growth of apiculate yeasts during the first days of ... | 2008 | 18457893 |
| synthesis, raman, ft-ir, nmr spectroscopic data and antimicrobial activity of mixed aza-oxo-thia macrocyclic compounds. | mixed aza-oxo-thia macrocyclic ligands 1,3,5,11,13,15-hexaaza-6,10,16,20-tetraoxo-8,18-dithia-2,3,4:12,13,14-dipyridine cyclocosane (l(1)); 1,3,5,12,14,16-hexeaza-6,11,17,22-tetraoxo-8,9,19,20-tetrathia-2,3,4:13,14,15-dipyridine cyclodocosane (l(2)); 1,3,5,13,15,17-hexaaza-6,12,18,24-tetraoxo-9,21-dithia-2,3,4:14,15,16-dipyridine cyclotetracosane (l(3)) and 1,3,5,14,16,18-hexaaza-6,13,19,26-tetraoxo-9,10,22,23-tetrathia-2,3,4:15,16,17-dipyridine cyclohexacosane (l(4)) were synthesised. the struc ... | 2009 | 18417256 |
| killer activity of yeasts isolated from natural environments against some medically important candida species. | twenty-five yeast cultures, mainly of human origin, belonging to four pathogenic yeast species--candida albicans, candida krusei, candida parapsilosis, and candida tropicalis were tested for their sensitivity to ten basidiomycetous and eleven ascomycetous yeast species isolated from the water and soil environments and from tree leaves. the best killer activity among basidiomycetous species was exhibited by rhodotorula glutinis, and r. mucilaginosa. the other carotenoid producing species cystofil ... | 2007 | 17419188 |
| characterization and comparison of microbial community of different typical chinese liquor daqus by pcr-dgge. | aims: to identify and compare microbiota in chinese liquor daqu, which were produced in the different regions using different production process. methods and results: the dna exacted from daqu samples was used as a template for pcr with universal primers of 16s rrna, 26s rrna and 18s rrna, respectively. the amplicons were analysed using denaturing gradient gel electrophoresis (dgge). it was observed that the bacterial dgge profile indicated high diversity and predominance of lactic acid bacter ... | 2011 | 21554340 |
| yeast populations residing on healthy or botrytis-infected grapes from a vineyard in attica, greece. | the yeast flora associated with healthy and botrytis-infected grapes was assessed. molecular identification methods assigned isolates to six genera and nine species. for the first time hanseniaspora opuntiae was encountered as an inhabitant of the grape ecosystem. by using drai, an informative restriction fragment length polymorphism pattern was generated to distinguish h. opuntiae from the closely related organism hanseniaspora guilliermondii. botrytis infection resulted in a larger population ... | 2007 | 17293525 |
| synthesis, characterization and antimicrobial activity of fe(ii), zn(ii), cd(ii) and hg(ii) complexes with 2,6-bis(benzimidazol-2-yl) pyridine ligand. | 2,6-bis(benzimidazol-2-yl)pyridine (l) ligand and complexes [m(l)cl(2)] and [fe(l)(2)](clo(4))(2) (m=zn, cd, hg) have been synthesized. the geometries of the [m(l)cl(2)] complexes were derived from theoretical calculation in dgauss/dft level (dzvp basis set) on cache. the central m(ii) ion is penta-coordinated and surrounded by n(3)cl(2) environment, adopting a distorted trigonal bipyramidal geometry. the ligand is tridentate, via three nitrogen atoms to metal centre and two chloride ions lie on ... | 2007 | 17189664 |
| use of molecular methods for the identification of yeast associated with table olives. | a molecular approach is used for the identification of yeast isolated from table olives. our results validate those obtained in the past by the classical biochemical methodology. yeast were isolated from both aerobically and anaerobically processed black table olives and also from canned seasoned green table olives. molecular identification methodology used included restriction pattern analysis of both pcr-amplified 5.8s rrna gene and internal transcribed spacers its(1) and its(2). for some spec ... | 2006 | 16943084 |
| [geographical races of certain species of ascomycetous yeasts in the moscow and novosibirsk regions]. | strains of three species of the ascomycetous yeasts hanseniaspora guilliermondii, torulaspora delbrueckii, and debaryomyces hansenii, isolated from the above-ground parts of plants in similar biocenoses of distant geographic regions (moscow and novosibirsk regions), have been investigated. the strains in each species were indistinguishable with respect to phenotypic features and general dna characteristics as determined by restriction analysis. however, comparison of the strains using pcr analys ... | 2005 | 16315988 |
| alcohols, esters and heavy sulphur compounds production by pure and mixed cultures of apiculate wine yeasts. | strains of hanseniaspora uvarum, hanseniaspora guilliermondii and saccharomyces cerevisiae were used as pure or mixed starter cultures in commercial medium, in order to compare their kinetic parameters and fermentation patterns. in pure and mixed cultures, yeasts presented similar ethanol yield and productivity. pure cultures of h. uvarum and s. cerevisiae showed a specific growth rate of 0.38 h(-1); however, this value decreased when these yeasts were grown in mixed cultures with h. guilliermon ... | 2005 | 16099313 |
| yeast populations associated with ghanaian cocoa fermentations analysed using denaturing gradient gel electrophoresis (dgge). | the yeast populations associated with the fermentation of ghanaian cocoa were investigated using denaturing gradient gel electrophoresis (dgge). samples were collected at 12-24 h intervals from heap and tray fermentations, at three different fermentation sites and different periods during the season. eukaryotic universal primers were used to amplify a fragment of the 26s rrna gene. the dgge profiles were relatively complex, underlining that the fermentation of cocoa is a complex microbial proces ... | 2005 | 15704234 |
| occurrence and diversity of yeasts involved in fermentation of west african cocoa beans. | samples of cocoa beans were taken on two separate occasions during heap and tray fermentations in ghana, west africa. in total 496 yeast isolates were identified by conventional microbiological analyses and by amplification of their its1-5.8s rdna-its2 regions. for important species the identifications were confirmed by sequencing of the d1/d2 domain of the 5' end of the large subunit (26s) rdna. assimilations of organic acids and other carbon compounds were conducted. for dominant yeasts intras ... | 2005 | 15691749 |
| hanseniaspora meyeri sp. nov., hanseniaspora clermontiae sp. nov., hanseniaspora lachancei sp. nov. and hanseniaspora opuntiae sp. nov., novel apiculate yeast species. | fourteen apiculate yeast strains isolated from various sources in south africa, north america and the hawaiian islands were found to be genetically divergent from other hanseniaspora-kloeckera species by using randomly amplified polymorphic dna (rapd)-pcr. after cluster analysis of the rapd-pcr fingerprints, five groups were recognized. dna reassociation values among representatives of these groups and strains of hanseniaspora-kloeckera species revealed that the strains represent five novel spec ... | 2003 | 13130067 |
| acetate ester formation in wine by mixed cultures in laboratory fermentations. | two non-saccharomyces wine yeast strains, hanseniaspora guilliermondii 11104 and pichia anomala 10590, selected as good producers of acetate esters when grown on synthetic microbiological medium, have been tested in wine fermentations as mixed cultures together with saccharomyces cerevisiae. wines produced using mixed cultures showed levels of acetaldehyde, acetic acid, glycerol and total higher alcohols within the ranges described for wine, whereas an increase in acetate ester concentrations wa ... | 2003 | 12892933 |
| physiological behaviour of hanseniaspora guilliermondii in aerobic glucose-limited continuous cultures. | the physiology of hanseniaspora guilliermondii was studied under aerobic glucose-limited conditions using the accelerostat procedure (continuous acceleration of dilution rate) and classical chemostat cultures. by both cultivation techniques this yeast was found to be crabtree-positive. up to a dilution rate of 0.25 h(-1), glucose was completely metabolised into biomass, glycerol and carbon dioxide. above this value, an increase in the dilution rate was accompanied by the production of other meta ... | 2003 | 12702454 |
| effects of low electric treatment on yeast microflora. | to contribute to the understanding of phenomena related to different intensity electric current treatments on the growth and metabolism of selected micro-organisms using laboratory samples of pure and co-cultures (saccharomyces cerevisiae strain 404 and hanseniaspora guilliermondii strain 465). | 2002 | 12392536 |
| studies on acetate ester production by non-saccharomyces wine yeasts. | a double coupling bioreactor system was used to fast screen yeast strains for the production of acetate esters. eleven yeast strains were used belonging to the genera candida, hanseniaspora, metschnikowia, pichia, schizosaccharomyces and zygosacharomyces, mainly isolated from grapes and wine, and two wine saccharomyces cerevisiae strains. the acetate ester forming activities of yeast strains belonging to the genera hanseniaspora (hanseniaspora guilliermondii and h. uvarum) and pichia (pichia ano ... | 2001 | 11764193 |
| molecular characterisation of hanseniaspora species. | the sequences of the internal transcribed spacers (its regions) and the 5.8s rrna gene, together with the electrophoretic karyotypes of 27 strains representative of the six species belonging to the genus hanseniaspora, were examined. from the analysis of the 5.8s rrna gene and the its regions, the genus hanseniaspora is monophyletic and can be divided into two subgroups. this subdivision was supported by electrophoretic chromosome patterns. hanseniaspora guilliermondii, h. uvarum and h. valbyens ... | 2001 | 11761370 |
| coflocculation of escherichia coli and schizosaccharomyces pombe. | several yeasts, such as candida utilis, dekkera bruxellensis, hanseniaspora guilliermondii, kloeckera apiculata, saccharomyces cerevisiae and schizosaccharomyces pombe, were found to coaggregate with escherichia coli, but s. pombe showed much less coflocculation than the other yeasts (peng et al. 2001)). s. pombe is known to have galactose-rich cell walls and we investigated whether this might be responsible for its different behavior by studying the wild-type tp4-1d, with a mannose to galactose ... | 2001 | 11693916 |
| flocculation and coflocculation of bacteria by yeasts. | biotransformations in natural environments frequently involve interactions between microorganisms. although there are many reports on the interactions between bacteria, interactions between yeasts and bacteria have not been extensively studied. previously we reported on the flocculation and coflocculation of pediococcus damnosus by saccharomyces cerevisiae. now we report that several other yeasts, such as candida utilis, dekkera bruxellensis, hanseniaspora guilliermondii, kloeckera apiculata, an ... | 2001 | 11525628 |
| glycerol and other fermentation products of apiculate wine yeasts. | ninety-six strains of apiculate wine yeasts were studied for their ability to produce glycerol, acetaldehyde, ethyl acetate, sulphur dioxide and hydrogen sulphide in synthetic medium. hanseniaspora guilliermondii produced smaller quantities of glycerol, acetaldehyde and hydrogen sulphide than kloeckera apiculata, whereas the production of ethyl acetate and sulphur dioxide was found to be similar. strains characterized by different capacities and properties were found for both species. the existe ... | 1997 | 9172403 |
| saccharomyces cerevisiae ccmi 885 secretes peptides that inhibit the growth of some non-saccharomyces wine-related strains. | the nature of the toxic compounds produced by saccharomyces cerevisiae ccmi 885 that induce the early death of hanseniaspora guilliermondii during mixed fermentations, as well as their ability to inhibit the growth of other non-saccharomyces wine-related strains, was investigated. the killing effect of mixed supernatants towards h. guilliermondii was inactivated by protease treatments, thus revealing the proteinaceous nature of the toxic compounds. analysis of the protein pattern of mixed supern ... | 2010 | 20039034 |
| biodiversity and phytase capacity of yeasts isolated from tanzanian togwa. | the focus of the present investigation was on the tanzanian fermented food togwa as a source for dietary iron and zinc, and the potential for mineral availability improvements using selected yeasts. to establish the content of target minerals and main inhibitor for intestinal uptake, iron and zinc as well as the mineral chelating phytic acid, (ip(6) or phytate) were determined in naturally fermented togwa. yeasts were isolated from sorghum, maize and cassava based togwa, and identified by sequen ... | 2010 | 19906458 |
| natural yeast flora of different varieties of grapes used for wine making in india. | the natural saccharomyces and non-saccharomyces yeast flora present on the grape berries significantly affect wine production. six grape varieties, bangalore blue, zinfandel, cabernet, chenin blanc, sauvignon blanc and shiraz are being used in india for wine making. the yeast diversity was studied on the basis of morphological, colony, physiological characteristics and 5.8s-its sequencing of rdna of the isolates. eleven different species belonging to seven genera were identified as: candida azym ... | 2009 | 19835764 |
| selection, growth, and chemo-sensory evaluation of flocculent starter culture strains of saccharomyces cerevisiae in the large-scale production of traditional brazilian cachaça. | the physiological and kinetic capabilities of 233 saccharomyces cerevisiae isolates, originating from traditional brazilian cachaça fermentation, were evaluated under laboratory conditions to select flocculent and non-h2s producing strains to be employed in beverage production. three flocculent s. cerevisiae strains were selected, two non-h2s producing and one h2s producing, and their kinetic performances were analysed during two large-scale fermentation experiments in a traditional cachaça dist ... | 2009 | 19329211 |
| synthesis, characterization and antimicrobial activity of water soluble dendritic macromolecules. | several families of water soluble dendrimers were synthesized based on poly(propyleneoxide) amines (jeffamines) (p(1)). p(1)-core and branched units were constructed from both methylacrylate and ethylenediamine (p(2)-p(9), and generations 0-3 with -nh(2), -cooh functionalities). they were characterized by elemental analysis (ea), gel permeation chromatography (gpc), ft-ir, (1)h, and (13)c nmr. the antimicrobial activities of only water soluble compounds (p(1), p(3), p(4), p(6), p(7) and p(9)) we ... | 2009 | 18657884 |
| yeast species associated with wine grapes in china. | having more information on the yeast ecology of grapes is important for wine-makers to produce wine with high quality and typical attributes. china is a significant wine-consuming country and is becoming a serious wine-producer, but little has been reported about the yeast ecology of local ecosystems. this study provides the first step towards the exploitation of the yeast wealth in china's vine-growing regions. the aim of this study was to investigate the yeast population density and diversity ... | 2010 | 20116124 |
| diversity of saccharomyces and non-saccharomyces yeasts in three red grape varieties cultured in the serranía de ronda (spain) vine-growing region. | for the first time, an ecological survey of wine yeasts present in grapes growing in two vineyards located in the region of "serranía de ronda" (málaga, southern spain) has been carried out. during the 2006 and 2007 vintages, grapes from different varieties were aseptically collected and allowed to ferment spontaneously in the laboratory. from a total of 1586 colonies isolated from microvinifications, 1281 were identified according to its polymorphisms and their identity confirmed by sequencing ... | 2010 | 20851489 |
| Analysis and direct quantification of Saccharomyces cerevisiae and Hanseniaspora guilliermondii populations during alcoholic fermentation by fluorescence in situ hybridization, flow cytometry and quantitative PCR. | Traditionally, it was assumed that non-Saccharomyces (NS) yeasts could only survive in the early stages of alcoholic fermentations. However, recent studies applying culture-independent methods have shown that NS populations persist throughout the fermentation process. The aim of the present work was to analyze and quantify Saccharomyces cerevisiae (Sc) and Hanseniaspora guilliermondii (Hg) populations during alcoholic fermentations by plating and culture-independent methods, such as fluorescence ... | 2011 | 21925033 |
| Degradation of phytate by Pichia kudriavzevii TY13 and Hanseniaspora guilliermondii TY14 in Tanzanian togwa. | The fermented cereal-based gruel togwa is used as weaning food for children in Tanzania. Togwa is rich in minerals but these are often not available for uptake in the human intestine due to natural inhibitors, such as phytate (IP(6)). The yeasts Pichia kudriavzevii TY13, Hanseniaspora guilliermondii TY14 and TY20, isolated from Tanzanian togwa, and selected for high phytase activity in complex yeast medium YPD, were now studied regarding their ability to degrade IP(6) in maize-based model togwa. ... | 2011 | 22112916 |
| the microbiology of ghanaian cocoa fermentations analysed using culture-dependent and culture-independent methods. | export of cocoa beans is of great economic importance in ghana and several other tropical countries. raw cocoa has an astringent unpleasant taste and a spontaneous fermentation is the first step in a process leading to cocoa beans with the characteristic cocoa flavour and taste. the microbiology of ghanaian cocoa fermentations was investigated using culture-dependent and culture-independent methods. samples were collected at 12 hour intervals during 96-144 hour tray and traditional heap fermenta ... | 2007 | 17161485 |