[study on polyphasic taxonomy of rhizobia isolated from lespedeza species].the diversity of rhizobia isolated from lespedeza spp. was determined on the basis of numerical analysis of phenotypic characteristics, sodium dodecyl sulfate-polyacrylarmide gel electrophoresis (sds-page) of proteins, dna-dna homology and restriction fragment length polymorphism (rflp) analysis of 16s-ribosomal dna genes. according to numerical analysis of 125 phenotypic characteristics, strains were divided into two groups at a similarity level of 67%. group i included all the fast-growing str ...199912555566
sericea lespedeza hay as a natural deworming agent against gastrointestinal nematode infection in goats.infection with gastrointestinal nematodes (gin), particularly haemonchus contortus, is the biggest constraint to profitable goat production in the united states (us). due to widespread prevalence of anthelmintic resistance in goat gin, alternative, non-chemical control methodologies are needed to increase profitability of small ruminant industries. a study was designed to test the efficacy of a high condensed tannin (ct) legume, sericea lespedeza [sl, lespedeza cuneata (dum.-cours. g. don)] agai ...200616564132
effect of sericea lespedeza (lespedeza cuneata) fed as hay, on natural and experimental haemonchus contortus infections in lambs.parasitic infection is one of the leading economic constraints in small ruminant production. this problem is exacerbated as the resistance of nematode populations to chemical treatment (anthelmintics) becomes increasingly more common. condensed tannin (ct) containing plants are being investigated as alternative solutions to address these problems. this investigation was conducted to evaluate the effect of the ct containing forage, lespedeza cuneata (sericea lespedeza, sl) fed as hay, on haemonch ...200616857320
effect of pelleting on efficacy of sericea lespedeza hay as a natural dewormer in goats.resistance of gastrointestinal nematodes (gin) to anthelmintic treatment has increased pressure to find alternative, non-chemical control methods. feeding hay of the high condensed tannin (ct) forage sericea lespedeza [sl; lespedeza cuneata (dum-cours.) g. don.] to sheep and goats has reduced gin fecal egg count (fec) and worm numbers in the abomasum and small intestines. this effect has been reported with both unground (long) and ground hay. pelleting of ground hay increases ease of storage, tr ...200717383822
affinity purification and characterization of a key enzyme responsible for circadian rhythmic control of nyctinasty in lespedeza cuneata l.the synthesis of an affinity gel aimed at leaf-opening factor beta-glucosidase (lofg) and affinity purification of lofg is presented. a gluconamidine-based beta-glucosidase inhibitor was used as the ligand of the affinity gel. beta-glucosidase exhibiting an activity shift throughout the day was selectively purified from lespedeza cuneata don by the affinity gel. the resulting lofg exhibited high substrate specificity toward the leaf-opening factor.200818308573
the effects of feeding sericea lespedeza hay on growth rate of goats naturally infected with gastrointestinal nematodes.goat production is increasing in the united states due to high ethnic demand, but infection with gastrointestinal nematode (gin) parasites is a major constraint to the industry. increasing gin resistance to chemical anthelmintics worldwide has led to the development of alternative control strategies, including use of forages containing condensed tannins (ct). an experiment was designed using infected and dewormed male kids (kiko x spanish, 6 mo old, 18.9 +/- 3.25 kg) fed diets containing 25% con ...200818469053
efficacy of sericea lespedeza hay as a natural dewormer in goats: dose titration study.gastrointestinal nematodes (gin) parasitism is the greatest threat to economic sheep and goat production in the southern usa, and there is widespread prevalence of gin resistance to broad-spectrum anthelmintics in this region. a natural alternative for controlling gin in small ruminants is feeding hay of sericea lespedeza [sl, lespedeza cuneata (dum.-cours., g. don)], a perennial warm-season legume high in condensed tannins. to determine the level of sl needed to reduce gin infection, a confinem ...200919464120
effect of feeding sericea lespedeza leaf meal in goats experimentally infected with haemonchus contortus.effect of sericea lespedeza [sl; lespedeza cuneata (dum-cours.) g. don.] leaf meal feeding was evaluated in two experiments in indoor reared goats with experimental infection of haemonchus contortus larvae. in the first experiment, ten 8-10 month old male spanish and alpine cross kids pair matched for body weight and age were fed sl or bermudagrass [bg; cynodon dactylon (l.) pers.] hay one week before infection and were infected with 5000 h. contortus l(3). the animals were maintained on the sam ...201021232867
dose titration of sericea lespedeza leaf meal on haemonchus contortus infection in lambs and kids.the objective of three experiments was to determine the impact of supplementing sericea lespedeza (lespedeza cuneata; sl) in three concentrations in a loose or pelleted diet on gastrointestinal nematodes (gin) in small ruminants. experiments on lambs were conducted at the usda, agricultural research service in booneville, ar (exp. 1) and at louisiana state university in baton rouge, la (exp. 2); an experiment on goat kids occurred at university of maryland-eastern shore (exp. 3). exp. 1 used cro ...201121621921
experiences with integrated concepts for the control of haemonchus contortus in sheep and goats in the united states.the generally warm, moist environmental conditions in the southern united states (u.s.) are ideal for survival and growth of the egg and larval stages of haemonchus contortus and other gastrointestinal nematodes (gin) of sheep and goats. consequently, infection with gin is the greatest threat to economic small ruminant production in this region. with anthelmintic resistance now reaching epidemic proportions in small ruminants in the u.s., non-chemical control alternatives are critically needed. ...201122178411
effect of sericea lespedeza leaf meal pellets on adult female haemonchus contortus in goats.sericea lespedeza (sl; lespedeza cuneata) is a perennial warm-season forage rich in condensed tannins (ct) that has been reported to have anthelmintic activity against small ruminant gastrointestinal nematodes (gin), particularly haemonchus contortus, a highly pathogenic blood-feeder, but the mechanism of action of ct against h. contortus is not clearly understood. an experiment with young goats was designed to study the effect of sl leaf meal pellets on (1) a mature h. contortus infection, and ...201525465738
effect of fall-grazed sericea lespedeza (lespedeza cuneata) on gastrointestinal nematode infections of growing goats.high prevalence of anthelmintic-resistant gastrointestinal nematodes (gin) in goats has increased pressure to find effective, alternative non-synthetic control methods, one of which is adding forage of the high condensed tannin (ct) legume sericea lespedeza (sl; lespedeza cuneata) to the animal's diet. previous work has demonstrated good efficacy of dried sl (hay, pellets) against small ruminant gin, but information is lacking on consumption of fresh sl, particularly during the late summer-autum ...201424996964
effect of molecular weight and concentration of legume condensed tannins on in vitro larval migration inhibition of haemonchus contortus.the effect of molecular weight of condensed tannins (ct) from a variety of warm-season perennial legumes commonly consumed by sheep and goats on anthelmintic activity has not been previously explored. the objectives of this study were to determine if molecular weight of ct from warm-season perennial legumes could predict the biological activity of ct relative to anthelmintic activity against ivermectin resistant l3 stage haemonchus contortus (hc) using a larval migration inhibition (lmi) assay. ...201424126088
effect of supplemental sericea lespedeza leaf meal pellets on gastrointestinal nematode infection in grazing goats.feeding sun-dried sericea lespedeza [sl; lespedeza cuneata (dum-cours.) g. don.] reduces gastrointestinal nematode (gin) infection in goats fed in confinement, but effects of this forage when fed as a supplement to goats on pasture are unclear. a study was completed in which supplemental feeds (75 and 95% sl leaf meal pellets and a commercial pellet, all fed at 0.91 kg/head/day) were offered to thirty growing male spanish goats (9 months old, 20.6 ± 2.8 kg, 10/treatment) grazing perennial warm-s ...201322985929
in vitro anthelmintic activity of crude extracts of selected medicinal plants against haemonchus contortus from sheep.ethanol extracts of 25 plant species were screened for anthelmintic effects against haemonchus contortus. ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°c with control cultures which were treated with ethanol for 48 h. five plants with high efficacies (ananas comosus, aloe ferox, allium sativum, lespedeza cuneata and warburgia salutaris) were selected from the first screening for further investigation using eth ...201322449714
reaction of field-grown sericea lespedeza to selected meloidogyne spp.five sericea lespedeza (lespedeza cuneata [dumont] g. don) breeding lines resistant to meloidogyne incognita, m. incognita acrita, and m. hapla as seedlings in greenhouse tests and two varieties were resistant to m. incognita acrita in field experiments. root-knot galling and larvae numbers were less for resistant entries than for the susceptible check when grown in root-knot infested field soil for three growing seasons. forage yields were as much as 57 times greater for resistant entries than ...197119322393
[pb, zn accumulation and nutrient uptake of 15 plant species grown in abandoned mine tailings].vegetation restoration field test was carried out in the abandoned lead-zinc tailings for 3 years. the study showed that accumulation of lead (pb), zinc (zn) and nutrient uptake differed with plant species and organs, heavy metals, and planting time. pb was mainly accumulated in tree roots, and its content distribution in tree organs was generally in the order of roots > leaves > stems. but zn concentrations in leaves of several tree species were higher than those in roots and stems. within the ...201222946191
flavor compounds and quality parameters of chevon as influenced by sericea lespedeza hay.this research assessed the utilization of sericea lespedeza (sl, lespedeza cuneata ) hay, a highly condensed tannin (ct) forage (87-181 g ct/kg), as a dietary regimen of meat goats, and thereby the effects on physicochemical properties of goat meat (chevon) and flavor compounds in cooked chevon chops were evaluated. although it is commonly believed that higher amounts of ct can have deleterious effects on animal performance due to low digestibility and low voluntary intakes in ruminants, feeding ...201222432963
phytoremediation potential of transplanted bare-root seedlings of trees for lead/zinc and copper mine tailings.selecting plant species that can overcome unfavorable conditions and increase the recovery of degraded mined lands remains a challenge. a pot experiment was conducted to evaluate the feasibility of using transplanted tree seedlings for the phytoremediation of lead/zinc and copper mine tailings. one-year-old bare-root of woody species (rhus chinensis mill, quercus acutissima carruth, liquidambar formosana hance, vitex trifolia linn. var. simplicifolia cham, lespedeza cuneata and amorpha fruticosa ...201627216539
anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on caenorhabditis elegans, and their antioxidant capacity.although tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (ct), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (ht). we evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either most ...201323102761
the biology and preliminary host range of megacopta cribraria (heteroptera: plataspidae) and its impact on kudzu growth.the bean plataspid, megacopta cribraria (f.), recently was discovered in the united states feeding on kudzu, pueraria montana lour. (merr.) variety lobata (willd.), an economically important invasive vine. we studied its biology on kudzu and its impact on kudzu growth. we also tested its ability to use other common forest legumes for oviposition and development. flight intercept traps operated from 17 may 2010 to 31 may 2011 in a kudzu field near athens, ga showed three peaks of adult flight act ...201222525058
yield and nutritive quality of sericea lespedeza (lespedeza cuneata) and little bluestem (schizachyrium scoparium) exposed to ground-level ozone.sericea lespedeza (lespedeza cuneata cv. interstate 76) and little bluestem (schizachyrium scoparium cv. aldous) were raised from seed in a glasshouse, transplanted into 5.7-l pots and placed into open-top chambers (otc) on 6 june 1999. following a 7-day adjustment period, each of six otcs (duplicate otcs per treatment) was ventilated with either air that had been carbon-filtered (cf) to remove ambient ozone (o3); non-filtered (nf), representative of ambient air; or enriched to twice-ambient o3 ...200312547521
allelopathic effects of juglone on germination and growth of several herbaceous and woody species.laboratory experiments were conducted to determine juglone sensitivity of 16 species (trifolium incarnatum, coronilla varia, vicia villosa, lespedeza stipulacea, l. cuneata, acer ginnala, caragana arbor-escens, elaegnus angustifolia, e. umbellata, lonicera maackii, quercus alba, fraxinus americana, liriodendron tulipifera, alnus glutinosa, pinus strobus, andp. sylvestris) being considered for mixed plantings withjugions nigra (black walnut). all species were sensitive to juglone, but seed germin ...198324407348
d-pinitol in fabaceae: an oviposition stimulant for the common grass yellow butterfly, eurema mandarina.the common grass yellow butterfly, eurema mandarina (formerly eurema hecabe mandarina) (lepidoptera, pieridae), recently has been separated taxonomically from a subtropical population of eurema hecabe in japan. this species is widely distributed in the temperate region of japan, and feeds mainly on various ligneous plants within the fabaceae. we attempted to identify an oviposition stimulant for e. mandarina from its primary hosts, albizia julibrissin and lespedeza cuneata. in both hosts, crude ...201627714574
supplemental polyethylene glycol affects intake of and preference for sericea lespedeza by cattle.we tested the hypothesis that supplemental polyethylene glycol (peg), a polymer that neutralizes the effects of tannins, would increase intake and preference of cattle for fresh-cut sericea lespedeza (sl; lespedeza cuneata), a legume with high concentrations of condensed tannins. sixteen crossbred steers (initial bw of 249 +/- 6.6 kg) were randomly assigned to 2 treatments: 1) grain supplement with peg (peg-s) and 2) grain supplement without peg (control). to assess intake, we conducted a trial ...200918849376
forage systems for cow-calf production in the appalachian region.small cow-calf operations are common in the appalachian region. tall fescue [lolium arundinaceum (schreb.) s. j. darbyshire] is the dominant forage in these systems for direct grazing as well as for stockpiling. the present study was conducted from 2001 to 2005. a total of 108 angus and angus crossbred cows were allotted randomly to 6 forage systems and then to 3 replicates within each system. in brief, system 1 had a stocking rate of 0.91 ha/cow in a middleburg 3-paddock (a, b, and c) system. s ...200818407993
characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose identify simple screening tools for selecting condensed tannin (ct)-containing forages as candidate sources for further study, ct were isolated from nine legumes, and their molecular weights (mw), chromophore production, capacity to precipitate bovine serum albumin (bsa) and fraction 1 protein (rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. sources were as follows: leaves of sericea lespedeza (lespedeza cuneata dum.-cours.), crown vetch (coronilla vari ...200516132212
the effect of grazing forage containing condensed tannins on gastro-intestinal parasite infection and milk composition in angora does.the objective of this study was to evaluate effects of the condensed tannin (ct)-containing forage sericea lespedeza (sericea lespedeza (sl); lespedeza cuneata; 15.2% ct), on fecal egg count (fec), larval development (larvae/10 g of feces), worm burden and immune response compared with a crabgrass (digitaria ischaemum)/kentucky 31 tall fescue (festuca arundinacea; control forage (ctf)) forage low in ct (0.32% ct) in grazing angora does and their kids. fifty worm-free mixed-age does were randomly ...200515893077
lc-ms-based chemotaxonomic classification of wild-type lespedeza sp. and its correlation with this study, 39 specimens belonging to lespedeza species (lespedeza cyrtobotrya, l. bicolor, l. maximowiczii, and lespedeza cuneata) (leguminosae) were classified phenotypically and genotypically. we constructed a phylogenetic tree based on the combined nrdna (internal transcribed spacer; its) and cpdna (trnl-trnf) sequences with the aim of classifying the genotypes. samples were mainly divided into three genotypes. samples of l. cyrtobotrya and l. bicolor were mixed in a single branch, wherea ...201222847333
herbaceous vegetation productivity, persistence, and metals uptake on a biosolids-amended mine soil.the selection of plant species is critical for the successful establishment and long-term maintenance of vegetation on reclaimed surface mined soils. a study was conducted to assess the capability of 16 forage grass and legume species in monocultures and mixes to establish and thrive on a reclaimed appalachian surface mine amended with biosolids. the 0.15-ha coarse-textured, rocky, non-acid forming mined site was prepared for planting by grading to a 2% slope and amending sandstone overburden ma ...201216151233
characterization of copper-resistant agrobacterium isolated from legume nodule in mining tailings.a copper-resistant bacteria ccnwsx2332 was isolated from root nodules of lespedeza cuneata growing in a gold mining tailing region in northwest of china. the specific growth rate of the strain was 0.62 microh(-1) in the presence of 2.0 mm cu(2+) in ty liquid media, and the maximum copper accumulation of whole cell reached 147.03 microm cu(2+) per gram (dry weight) after 4 h incubation. a partial sequence of the copper resistance gene copa was amplified from the strain, and the phylogenetic analy ...200918979058
restoration of drastically eroded land using coal fly ash and poultry biosolid.a 3-year field study was conducted at a 12 ha soil-borrow area adjacent to the columbia metropolitan airport, south carolina to investigate the restorative effects of co-application of coal fly ash (fa) and a poultry biosolid (pb). fa was applied at 0, 22, 280, 560 and 1120 mg (tonne) ha(-1), and pb at 5 and 10 mg ha(-1). the area was seeded with erosion-control species atlantic coastal panic grass (panicum amarum var amarum l.), sericea (lespedeza cuneata var. appalow [dumont] g. don.) and weep ...200212398338
in vivo effect of selected medicinal plants against gastrointestinal nematodes of sheep.nematode resistance to anthelmintic drugs affects small ruminant production globally. this study evaluated in vivo effects of five plant extracts as alternative nematode treatments. animals were divided into six groups. each group was randomly assigned a treatment: abamectin and praziquantel (cap) (positive control), ethanol extracts of ananas comosus (ac), aloe ferox (af), allium sativum (as), lespedeza cuneata (lc) and warburgia salutaris (ws). these were applied as an oral dose (100 mg kg(-1) ...201424293151
isolation and characterization of the heavy metal resistant bacteria ccnwrs33-2 isolated from root nodule of lespedeza cuneata in gold mine tailings in china.a total of 108 strains of bacteria were isolated from root nodules of wild legumes growing in gold mine tailings in northwest of china and were tested for heavy metal resistance. the results showed that the bacterial strain ccnwrs33-2 isolated from lespedeza cuneata was highly resistant to copper, cadmium, lead and zinc. the strain had a relatively high mean specific growth rate under each heavy metal stress test and exhibited a high degree of bioaccumulation ability. the partial sequence of the ...200918562095
detecting invasive sericea lespedeza (lespedeza cuneata) in mid-missouri pastureland using hyperspectral imagery.missouri ranks second in cow-calf numbers in the united states and its pastureland has experienced invasion of various plant species. as one of the invasive weeds, sericea lespedeza is becoming a serious threat to pasturelands in this state. the in-situ field survey in these vast pastures is time consuming and often impossible because of accessibility. typical aerial survey is also difficult to detect sericea because the plant is of similar size and color as natural grass and, thus, cannot be ef ...200818363054
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