Publications

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a pospiviroid from symptomless portulaca plants closely related to iresine viroid 1.in symptomless plants of portulaca a potential new pospiviroid was characterized. analysis by both double and return page showed the presence of a circular rna. rt-pcr and sequencing revealed a genome of 351 nt with properties characteristic of members of the genus pospiviroid and with highest sequence identity (circa 80%) with iresine viroid 1 (irvd-1). the circular rna from portulaca was shown to replicate independently in its original host, thus showing that it is indeed a viroid. based on it ...201525987478
a single polyprobe for detecting simultaneously eight pospiviroids infecting ornamentals and vegetables.the spread of viroids belonging to the genus pospiviroid (family pospiviroidae), recorded recently in ornamentals and vegetables in several european countries, calls for fast, efficient and sensitive detection methods. based on bioinformatics analyses of sequence identity among all pospiviroids, a digoxigenin-labeled polyprobe (pospiprobe) was developed that, when tested by dot-blot and northern-blot hybridization, detected potato spindle tuber viroid, citrus exocortis viroid, columnea latent vi ...201222935607
an rt-pcr primer pair for the detection of pospiviroid and its application in surveying ornamental plants for viroids.a primer pair for reverse transcription-polymerase chain reaction (rt-pcr), based on the conserved sequences of the members of genus pospiviroid was designed to yield a fragment of about 200 base pairs (bp). since pospiviroids infect a large number of plants species and a few members of the genus pospiviroid have been already detected in some ornamental plants, the primer pair was evaluated for its efficacy using ornamental plants. the method of return-polyacrylamide gel electrophoresis (r-page) ...200414738987
the molecular structure of iresine viroid, a new viroid species from iresine herbstii ('beefsteak plant').a viroid was isolated from iresine herbstii plants using the bidirectional page method for analysis of small circular rna molecules. the viroid was transmitted to viroid-free iresine herbstii plants of the cultivar 'aureoreticulata' by mechanical inoculation. infected plants did not develop any symptoms. the new viroid species has been named iresine viroid (irvd). it is a member of the potato spindle tuber viroid group and consists of 370 nucleotides, 227 g+c, 143 a+u (gc content, 61.4%). the mo ...19968887500
survey and analysis of simple sequence repeats (ssrs) present in the genomes of plant viroids.extensive simple sequence repeat (ssr) surveys have been performed for eukaryotic prokaryotic and viral genomes, but information regarding ssrs in viroids is limited. we undertook a survey to examine the presence of ssrs in viroid genomes. our results show that the distribution of ssrs in viroids may influence secondary structure, and that ssrs could play a role in generating genetic diversity. we also discuss the potential evolutionary role of repeated sequences in the viroid genome. this is th ...201424649400
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