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the p gene of the porcine paramyxovirus lpmv encodes three possible polypeptides p, v and c: the p protein mrna is edited.the nucleotide sequence of the p gene of the porcine paramyxovirus la-piedad-michoacan-mexico virus (lpmv) was analysed. three long open reading frames (orfs) were found in the mrna sense. insertion of two g residues is necessary to obtain an orf encoding the p protein, which gives a p protein of 404 amino acids with a calculated mr of 42475. this form of editing was demonstrated, two non-templated g residues being added in a portion of the mrna transcripts. the lpmv v protein, which has a conse ...19921588322
a comparative study on the use of virus and antibody detection techniques for the diagnosis of la piedad michoacan paramyxovirus (lpmv) infection in pigs.la piedad michoacan paramyxovirus (lpmv) is newly recognized paramyxovirus that has been associated with neurologic and reproductive disorders in pigs in mexico. to date, no comparative study of methods for the diagnosis of infection with this virus has been published. in this study, we identified tissues containing maximum virus load to optimize virus isolation procedures, and we compared this method to a rapid diagnostic test employing immunostaining of impression smears for lpmv antigens. in ...19979087918
development of a blocking elisa for screening antibodies to porcine rubulavirus, la piedad michoacan virus.a blocking enzyme-linked immunosorbent assay (elisa) was developed to detect antibodies to porcine rubulavirus (la piedad michoacan virus [lpmv]) in serum samples from pigs. the test, based on a monoclonal antibody against the lpmv hemagglutinin-neuraminidase glycoprotein, had a sensitivity of 99% and a specificity of 97%. the results of this test were in agreement with those obtained by an indirect elisa and hemagglutination inhibition, indirect immunofluorescence, and virus neutralization test ...199910424646
both the p and v proteins of the porcine rubulavirus lpmv interact with the np protein via their respective c-terminal unique parts.in this paper we show that the porcine rubulavirus lpmv phosphoprotein (p) and v protein (v) both interact with the nucleoprotein (np). there are also indications for an interaction between p and v with l protein. further analysis of the domains of the p and v which are necessary for interaction with the np protein demonstrates that the interaction is not mediated from their common part but instead from their unique c-terminal parts, respectively. the common n-terminus of p and v appear to media ...200211864739
apoptosis in lymph nodes and changes in lymphocyte subpopulations in peripheral blood of pigs infected with porcine rubulavirus.in a first experiment, five pigs were inoculated intranasally with porcine rubulavirus (porv) at 5 days of age and killed 7 days post-infection (pi). in a second experiment, four pigs were infected with the same virus at 17 days of age and killed at 9 or 15 days pi. control piglets in each experiment received uninfected cell culture supernate. all porv-infected pigs developed respiratory and nervous signs, and histological lesions of non-suppurative encephalitis and interstitial pneumonia. all c ...200312531681
experimental porcine rubulavirus (la piedad-michoacan virus) infection in pregnant gilts.porcine rubulavirus (la piedad-michoacan virus) (porv-lpmv) is a member of the paramyxoviridae family that causes encephalitis in young piglets and infertility in adult sows and boars. infertility in sows naturally infected by porv-lpmv is characterized by an increased number of returns to oestrus, stillbirths and mummified fetuses. in this study, nine seronegative gilts were inoculated intranasally with the pac-3 strain of porv-lpmv at week 6 or 10 of gestation. these animals were then killed a ...200414693118
generation and characterization of monoclonal antibodies against the flounder paralichthys olivaceus rhabdovirus.two mabs (3c7 and 3c9) against flounder paralichthys olivaceus rhabdovirus (porv) were generated with hybridoma cell fusion technology and characterized by an indirect enzyme-linked immunosorbent assay, isotype test, western blot and immunodot analysis and immunofluorescence assay. isotyping tests demonstrated that both of the two mabs belonged to igm subclass. western blot analysis showed the mabs reacted with 42, 30, and 22 kda viral proteins, which were localized within the cytoplasm of porv- ...200818191468
development and characterization of monoclonal antibodies to spring viraemia of carp virus.five monoclonal antibodies (mabs) against spring viraemia of carp (svcv0504, isolated from common carp in china) were produced from mice immunized with purified virus preparations. the virion of svcv contains five structural proteins, representing the nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g) and rna-dependent rna polymerase (l). western blotting analysis revealed that three mabs (1h5, 1e10, and 1h7) recognized specifically to a single protein of 47kda (n), the ...200818378003
molecular characterization of the hemagglutinin-neuraminidase gene of porcine rubulavirus isolates associated with neurological disorders in fattening and adult pigs."blue eye disease" is a viral infection of swine endemic in mexico, which produces fatal encephalitis accompanied by respiratory signs and corneal opacity in suckling piglets. an atypical blue eye disease outbreak presented high rates of neurological signs in fattening and adult pigs from 2000 to 2003. in order to identify the basis of increased neurovirulence, the hemagglutinin-neuraminidase (hn) gene of several porcine rubulavirus isolates were sequenced and compared with that of la piedad mic ...200818031776
identification of potential b cell epitope determinants by computer techniques, in hemagglutinin-neuraminidase from the porcine rubulavirus la piedad michoacan.hemagglutinin-neuraminidase (hn) from porcine rubulavirus la piedad michoacan (rvplpm) is one of the most antigenic proteins known, and is responsible for virus-host cell interaction. we analyzed the amino acid sequence of hn, using computer-assisted techniques to identify b cell epitopes. from a pool of 18 possible antigenic peptides, we evaluated the antigenicity of the 2 peptides with the highest scores and the 1 with lowest score. antibodies from rvplpm-infected pigs recognized the synthesiz ...200717603842
multiplex pcr and multiplex rt-pcr for inclusive detection of major swine dna and rna viruses in pigs with multiple infections.multiplex pcr and multiplex rt-pcr were developed to identify nine viruses in pigs with multiple infections. these viruses are: porcine circovirus type 2 (pcv2), suid herpesvirus 1, porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus, porcine rotavirus a (porv-a), porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and getah virus. these methods were shown to be high specificity and sensitivity. in the ...200919467264
semen alterations in porcine rubulavirus-infected boars are related to viral excretion and have implications for artificial insemination.porcine rubulavirus (porv), also known as blue eye disease (bed) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. to study the effect of porv infection on semen production, five boars were infected with 1 x 10(6) tcid(50)/ml of porv strain pac-3 and evaluated for 59 days post inoculation (dpi). infected boars developed reproductive tract pathology that included swelling of the testes and epididymides. analysis of the semen showed that the infection ...200717368687
blue eye disease porcine rubulavirus (porv) infects pig neurons and glial cells using sialo-glycoprotein as receptor.pig neural cells express glycoproteins with sialylated n-linked oligosaccharide chains (snoc) which are used by the porcine rubulavirus (porv) as receptors. pig neuronal or glial cell cultures were employed to investigate (a) whether porv infects such cells using a molecule expressing snoc, and (b) the role of viral envelope glycoproteins in establishing the infection. enriched neuronal or glial cell cultures were exposed to porv and infection was detected immunocytochemically. neuronal cultures ...200716459111
serological survey of veterinarians to assess the zoonotic potential of three emerging swine diseases in mexico.we conducted an immunological assay of blood samples taken from 85 swine-specialist veterinarians attending the congress of the mexican association of swine specialist veterinarians in mexico in 2011. serum samples were assayed for porcine rubulavirus (porpv), encephalomyocarditis virus (emcv) and leptospira spp. antibodies. using a hemagglutination inhibition test, we registered 2.3% and 27% seropositivity for porpv and emcv, respectively. using viral neutralization tests, we registered 5.8% an ...201423734711
purification of the porcine rubulavirus attachment protein by liquid isoelectric focusing.porcine rubulavirus (porv) is an emerging virus responsible for meningoencephalitis, respiratory distress, and reproductive alterations in pigs. the hemagglutinin-neuraminidase (hn) glycoprotein is the most exposed and antigenic of the virus proteins. hn plays central roles in porv infection; i.e., it recognizes sialic acid-containing cell receptors that mediate virus attachment and penetration; in addition, its neuraminidase (sialic acid hydrolysis) activity has been proposed to be a virulence ...200415039074
peanut and amaranthus leucocarpus lectins discriminate between memory and naive/quiescent porcine lymphocytes.lectins are relevant tools to isolate and characterize different cellular sub-populations. in this work, we used the lectins arachis hypogaea (peanut agglutinin, pna) and amaranthus leucocarpus (all), specific for galss1, 3galnac, to characterize naive and memory lymphocytes from pigs, experimentally infected with the porcine rubulavirus (rvp). our results showed that both lectins recognized preferentially lymphocytes with the cd4(+)cd8(+) phenotype (p<0.05). the phenotypic analysis of the cells ...200211825599
uptake of porcine rubulavirus (lpmv) by pk-15 cells.the porcine virus denominated la piedad michoacan virus (lpmv) is a member of the family paramyxoviridae and is the cause of a disease in pigs present only in mexico. the disease is characterized by meningoencephalitis and respiratory distress in young pigs, epididymitis and orchitis in boars, and reproductive failure and abortion in sows.200111578755
purification and characterization of the hemagglutinin-neuraminidase of porcine rubulavirus lpmv.the hemagglutinin-neuraminidase (hn) from the lpmv strain of porcine rubulavirus was purified from virions by ultracentrifugation in a continuous 20-60% sucrose gradient and by ion exchange chromatography. the hn is a glycoprotein of 66 kda constituted by 50.5, 13.3 and 13.6% of non polar, uncharged polar, and charged polar amino acids, respectively. the hn contains 4% of carbohydrates, its glycannic portion is constituted by man, gal, glcnac, galnac, and neu5ac in 3:3:4:1:1 molar ratios. the hn ...199910815988
porcine rubulavirus lpmv rna persists in the central nervous system of pigs after recovery from acute infection.in order to study persistence of the porcine rubulavirus lpmv, we examined tissue samples collected from pigs 53 days after experimental infection. these pigs survived the initial infection and could clinically be considered to have recovered from the infection. two of the pigs used in this study were chemically immunosuppressed during the last 4 days before necropsy. no infectious virus or viral antigen could be detected in any tissue using standard methods for virus isolation and detection. ho ...19989839652
multiple factors including subgenomic rnas and reduced viral protein expression are associated with a persistent infection by porcine rubulavirus (lpmv).the synthesis of virus specific rna and the expression of viral proteins in pk-15 cells persistently infected with the porcine rubulavirus lpmv have been studied at two different cell-passages following establishment of persistency (passages 25 and 65). protein analysis of persistently infected cells and the virus particles released from these failed to demonstrate the presence of the polymerase (l) protein. a decrease in the amount of the phospho- (p) protein was also noted. the genome and mrna ...19989572545
secondary structure prediction of the hemagglutinin-neuraminidase from a porcine rubulavirus.the hemagglutinin-neuraminidase (hn) from 'la piedad, michoacan' porcine rubulavirus (lpmv) interacts specifically with neuac alpha 2,3 lactose residues on the target cell. in this work we report the secondary structure of this protein, determined with five different theoretical algorithms. results indicate that the hn protein is organized in: an intracellular region (from amino acid 1 to 25); in a beta-strand transmembrane region (residue 26 to 47), typically hydrophobic, rigid and solvent inac ...19989541617
lesions in the reproductive tract of boars experimentally infected with porcine rubulavirus."blue eye" disease of pigs in mexico is caused by porcine rubulavirus and characterized by infertility in sows and boars, nervous signs in young pigs, and corneal opacity in pigs of all ages. the pathogenesis of reproductive tract lesions in rubulavirus-infected boars has not previously been investigated. in a first experiment, four 9-month-old boars were inoculated with porcine rubulavirus and killed 5, 15, 30 or 45 days post-inoculation (pi). in a second experiment, four similar boars were ino ...19979447484
establishment and characterisation of a porcine rubulavirus (lpmv) persistent infection in porcine kidney cells.porcine rubulavirus (lpmv) can establish persistent infections in porcine kidney cells. cell cultures characterised at passages 25 and 65 demonstrated haemadsorption, formation of syncytia, and a slower growth rate. the nucleoprotein (np) and haemagglutinin-neuraminidase (hn) protein were present in all cells, although not to the same extent as in wild type infected cells. incubation of the cell cultures with virus neutralising antibodies could not cure them from the infection. the cells were re ...19979444775
analysis of the large (l) protein gene of the porcine rubulavirus lpmv: identification of possible functional domains.the complete nucleotide sequence of the porcine rubulavirus lpmv (la piedad michoacan virus) large (l) protein gene was determined and analysed. the l mrna was found to span 6,786 nucleotides, containing one single large open reading frame (orf), putatively encoding a polypeptide of 2,251 amino acids. by aligning the amino acid sequence of the lpmv l-protein with l-protein of a number of viruses belonging to the order mononegavirale, a high degree of similarity between the lpmv l-protein and oth ...19979140194
a sequential study of experimental porcine paramyxovirus (lpmv) infection of pigs: immunostaining of cryostat sections and virus isolation.la piedad michoacan paramyxovirus (lpmv) is a recently recognized paramyxovirus infecting pigs throughout mexico. disease syndromes observed in field cases associated with lpmv infection include neurologic, respiratory, and reproductive disorders. clinical signs and the distribution of lpmv virus and antigen in tissue samples from pigs experimentally infected with lpmv by natural routes were studied. severe neurologic disease and death occurred following experimental inoculation of 3- and 17-day ...19968953523
the structural proteins of a porcine paramyxovirus (lpmv).the porcine paramyxovirus is a newly identified agent of a fatal disease in piglets, endemic in mexico since 1980, where it was seen around the town of la piedad, michoacan, mexico (hence lpm virus). at least six [35s]methionine-labelled proteins could be resolved by sds-page and five of them were clearly immunoprecipitated. selective labelling of lpmv-infected cells with [3h]glucosamine revealed two bands with an mr of about 66k and 59k, corresponding to the two viral glycoproteins, the haemagg ...19902313267
identification of the porcine paramyxovirus lpmv matrix protein gene: comparative sequence analysis with other paramyxoviruses.the complete nucleotide sequence of the gene encoding the matrix protein (m) of the porcine paramyxovirus lpmv has been determined. the gene is 1376 nucleotides long including 5' and 3' non-coding sequences with a protein-coding sequence of 1107 nucleotides. the deduced protein, containing 369 amino acids with a calculated mr of 41,657, is hydrophobic overall with a net positive charge of +17.5. comparative sequence analysis revealed high amino acid homology to other paramyxovirus m proteins, wi ...19912033388
the molecular biology of the porcine paramyxovirus lpmv.protein and genomic studies of a previously uncharacterized porcine paramyxovirus (designated lpmv) confirmed that it was a member of the paramyxovirus genus. the nucleotide sequences and deduced amino acids of the complete p-gene, m-gene, f-gene and hn-gene as well as the intergenic sequences have been determined. comparative sequence analysis of the m-gene of lpmv revealed the closest relationship of lpmv was to human mumps virus with a homology of 46% and 55% at the amino acid and nucleotide ...19921481359
investigation of t-cell responses and viral mrna persistence in lymph nodes of pigs infected with porcine rubulavirus.selected lymphocyte subpopulations were studied and the distribution of viral mrna were investigated during acute and persistent porcine rubulavirus (porv-lpmv) infection in vietnamese pot-bellied pigs. six pigs infected with porv-lpmv at 17 days of age exhibited clinical signs 7-10 days post-inoculation (pi). one infected piglet died 11 days pi while the other five recovered around day 13 pi and survived until euthanasia on day 277 pi. increased numbers of cd8+, cd4+ and cd2+ t cells were detec ...200918986711
[biological characterization of three natural isolates of the porcine rubulavirus (mexico)].biological characterization of three natural isolates of the porcine rubulavirus (mexico). porcine rubulavirus (porv) produces a neurological and reproductive syndrome in pigs called the blue-eye disease, known only from mexico. several isolates were grouped by the main symptoms presented during outbreaks: a) neurotropic in piglets, (b) broadly neurotropic in piglets and gonadotropic in adults, and (c) gonadotropic in adults. we studied some biological properties of three strains, which fall in ...200819256422
full-length genome sequence and genetic relationship of two paramyxoviruses isolated from bat and pigs in the americas.mapuera virus (mprv) was isolated from a fruit bat in brazil in 1979, but its host range and disease-causing potential are unknown. porcine rubulavirus (porv) was identified as the aetiological agent of disease outbreaks in pigs in mexico during early 1980s, but the origin of porv remains elusive. in this study, the completed genome sequence of mprv was determined, and the complete genome sequence of porv was assembled from previously published protein-coding genes and the non-coding genome regi ...200717385069
identification of antigenic variants of the porcine rubulavirus in sera of field swine and their seroprevalence.we sampled sera from 1013 non-vaccinated swine from four states in mexico, guanajuato, jalisco, michoacán and the estado de mexico, to analyse anti-porcine rubulavirus antibody titres against three different porcine rubulavirus isolates (pac-4/1993, pac-6/2001, and pac-9/2003) using a hemagglutination inhibition assay. the results revealed that there were antigenic differences among the isolates assessed. in particular, the estimated correlation between the pac-4/1993 and pac-6/2001 (0.50) isola ...201122171991
comparative evaluation of the cd4+cd8+ and cd4+cd8- lymphocytes in the immune response to porcine rubulavirus.the porcine immune system is unique in the expression of cd4+cd8+ (double-positive, dp) lymphocytes. these cells have been associated with immunological memory due to their gradual increase with age, the expression of memory phenotype and their ability to respond to recall viral antigen. this work analyzes the biological function of cd4+cd8- and cd4+cd8+ lymphocytes in the immune response to porcine rubulavirus (prv). cd4+cd8- cells isolated from pigs 3 weeks after infection with porcine rubulav ...200111389959
immunity to porcine rubulavirus infection in adult swine.the immune response against the porcine rubulavirus was analyzed in experimentally infected adult pigs. high titers of virus neutralizing and hemagglutinating inhibitory antibodies were identified in infected animals. the antibody specificity was directed towards hn, m, and np rubula virion proteins; immunodominance of hn proteins was demonstrated. peripheral blood mononuclear cells from infected, but not from non-infected pigs proliferated in vitro in response to virus antigenic stimuli, showin ...19989764728
neuac alpha 2,3gal-glycoconjugate expression determines cell susceptibility to the porcine rubulavirus lpmv.relevance of membrane sialoglycoconjugates as receptors for infection by the porcine rubulavirus has been determined in vitro by sugar and lectin competition assays and by inhibition of glycosylation. our results show that neuac alpha 2,3gal but not neuac alpha 2,6gal inhibits the virus infectivity of vero cells, and the virus was effectively blocked with the lectin maackia amurensis, specific for neuac alpha 2,3gal. inhibition of the cellular glycosylation with tunicamycin, deoxinojirimycin as ...19979440225
analysis of the fusion protein gene of the porcine rubulavirus lpmv: comparative analysis of paramyxovirus f proteins.complementary dna clones representing the fusion (f) protein gene of the porcine rubulavirus lpmv were isolated and sequenced. the f gene was found to be 1,845 nucleotides long containing one long open reading frame capable of encoding a protein of 541 amino acids. the cleavage motif for f0 into f1 and f2 is his-arg-lys-lys-arg. a sequence comparison and a phylogenetic analysis was performed in order to identify possible functional domains of paramyxovirus fusion proteins and also to classify th ...19979208455
the haemagglutinin-neuraminidase glycoprotein of the porcine paramyxovirus lpmv: comparison with other paramyxoviruses revealed the closest relationship to simian virus 5 and mumps virus.the complete nucleotide sequence of the haemagglutinin-neuraminidase (hn) gene of the porcine paramyxovirus lpmv, was determined from cdna derived from viral genomic rna. the gene was 1906 nucleotides long including a putative gene end and poly a signal. one long open reading frame was found encoding a protein of 576 amino acids with a calculated molecular weight of 63,324. the protein contains four potential n-glycosylation sites and a major hydrophobic region near the n-terminal, suggesting a ...19921731697
completion of the full-length genome sequence of human parainfluenza virus types 4a and 4b: sequence analysis of the large protein genes and gene start, intergenic and end sequences.we have already reported the nucleotide sequences of the np, p/v, m, f and hn genes of human parainfluenza virus type 4a (hpiv-4a) and type 4b (hpiv-4b). here, we have determined the sequences of the l protein genes as well as the gene start, intergenic and end sequences, thereby completing the full-length genome sequence of hpiv-4a and 4b. hpiv-4a and 4b have 17,052 and 17,304 nucleotides, respectively. the end sequence of hpiv-4, especially 4b, was extraordinarily long. in a comparison with me ...201020963613
cloning, expression and characterization of potential immunogenic recombinant hemagglutinin-neuraminidase protein of porcine rubulavirus.blue eye disease caused by porcine rubulavirus (porpv) is an endemic viral infection of swine causing neurological and respiratory disease in piglets, and reproductive failure in sows and boars. the hemagglutinin-neuraminidase (hn) glycoprotein of porpv is the most abundant component in the viral envelope and the main target of the immune response in infected animals. in this study, we expressed the hn-porpv-recombinant (rhn-porpv) protein in an escherichia coli system and analyzed the immune re ...201627496728
co-infection of classic swine h1n1 influenza virus in pigs persistently infected with porcine rubulavirus.porcine rubulavirus (porpv) and swine influenza virus infection causes respiratory disease in pigs. porpv persistent infection could facilitate the establishment of secondary infections. the aim of this study was to analyse the pathogenicity of classic swine h1n1 influenza virus (swh1n1) in growing pigs persistently infected with porcine rubulavirus. conventional six-week-old pigs were intranasally inoculated with porpv, swh1n1, or porpv/swh1n1. a mock-infected group was included. the co-infecti ...201626854342
full-genome sequencing and phylogenetic analysis of four neurovirulent mexican isolates of porcine rubulavirus.we report the complete genome sequences of four neurovirulent isolates of porcine rubulavirus (porpv) from 2015 and one historical porpv isolate from 1984 obtained by next-generation sequencing. a phylogenetic tree constructed using the individual sequences of the complete hn genes of the 2015 isolates and other historical sequences deposited in the genbank database revealed that several recent neurovirulent isolates of porpv (2008-2015) cluster together in a separate clade. phylogenetic analysi ...201728185101
acute neurologic disease in porcine rubulavirus experimentally infected piglets.the objective of this study was to evaluate the clinical disease, humoral response and viral distribution of recent porcine rubulavirus (porpv) isolates in experimentally infected pigs. four, 6-piglet (5-days old) groups were employed (g1-84, g2-93, g3-147, and g4-t). three viral strains were used for the experimental infection: the reference strain lpmv-1984 (michoacán 1984) and two other strains isolated in 2013, one in queretaro (qro/93/2013) and the other in michoacán (mich/147/2013). each s ...201728104449
production of an enzymatically active and immunogenic form of ectodomain of porcine rubulavirus hemagglutinin-neuraminidase in the yeast pichia pastoris.blue-eye disease (bed) of swine is a viral disease endemic in mexico. the etiological agent is a paramyxovirus classified as porcine rubulavirus (porv-lpmv), which exhibits in its envelope the hemagglutinin-neuraminidase (hn) glycoprotein, the most immunogenic and a major target for vaccine development. we report in this study the obtaining of ectodomain of porv hn (ehn) through the pichia pastoris expression system. the expression vector (ppiczαb-hn) was integrated by displacement into the yeas ...201626940828
molecular characterisation of porcine rubulavirus (porpv) isolates from different outbreaks in mexico.since the report of the initial outbreak of porcine rubulavirus (porpv) infection in pigs, only one full-length genome from 1984 (porpv-lpmv/1984) has been characterised. to investigate the overall genetic variation, full-length gene nucleotide sequences of current porpv isolates were obtained from different clinical cases of infected swine. genome organisation and sequence analysis of the encoded proteins (np, p, f, m, hn and l) revealed high sequence conservation of the np protein and the expr ...201626728078
molecular and epidemiological studies of porcine rubulavirus infection - an overview.porcine rubulavirus-la piedad-michoacan-mexico virus (porpv-lpmv) was identified as the causative agent of a viral disease that emerged spontaneously in mexican swine in the 1980s. since the report of the initial outbreak of the disease, only one full-length genome from a strain isolated in 1984 (porpv-lpmv/1984) has been sequenced; sequence data are scarce from other isolates. the genetic variation of this virus that has spread throughout the main endemic region of mexico is almost a complete m ...201526584829
la piedad michoacán mexico virus v protein antagonizes type i interferon response by binding stat2 protein and preventing stats nuclear translocation.la piedad michoacán mexico virus (lpmv) is a member of the rubulavirus genus within the paramyxoviridae family. lpmv is the etiologic agent of "blue eye disease", causing a significant disease burden in swine in mexico with long-term implications for the agricultural industry. this virus mainly affects piglets and is characterized by meningoencephalitis and respiratory distress. it also affects adult pigs, causing reduced fertility and abortions in females, and orchitis and epididymitis in males ...201626546155
long-term rna persistence of porcine rubulavirus (porpv-lpmv) after an outbreak of a natural infection: the detection of viral mrna in sentinel pigs suggests viral transmission.the persistence of porcine rubulavirus (porpv-lpmv) in five pigs that had survived an outbreak of a natural infection was determined. after the resolution of the outbreak, each animal was housed in an isolation pen together with one sentinel pig. approximately every 2 months thereafter one group of animals was euthanized and tissue samples taken for virological and serological analysis. infectious virus was not isolated from any samples; antibodies to porpv-lpmv were detected in convalescent pig ...201424768705
respiratory disease in growing pigs after porcine rubulavirus experimental infection.the aim of this study was to analyze the pathogenicity and distribution of porcine rubulavirus (porpv) in the respiratory tract of experimentally infected pigs. nine 6-week-old pigs were infected with porpv and examined clinically. blood, nasal swab, and tissue samples were collected on different days post-infection (dpi). the humoral immune responses and viral loads were evaluated. the infected pigs exhibited an increase in the respiratory clinical signs. in addition, the excretion of porpv was ...201323770154
efficacy of quantitative rt-pcr for detection of the nucleoprotein gene from different porcine rubulavirus strains.blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (porv). we have developed a qrt-pcr method to detect and quantify expression of the nucleoprotein gene for different porv strains. the limit of detection for this assay was 10(2) copies of synthetic rna. viral rna from porv was detectable at a tcid50 of 0.01. significant differences were observed between viral rna quantification and virus titration results for nine porv strains. for nasal and oral swab samples th ...201323525730
development of a real-time rt-pcr method for detection of porcine rubulavirus (porv-lpmv).in order to provide a rapid and sensitive method for detection of the porcine rubulavirus la piedad-michoacan-mexico virus (porv-lpmv), we have developed a specific real-time reverse transcriptase polymerase chain reaction assay. the detection of porv-lpmv, represents a diagnostic challenge due to the viral rna being present in very small amounts in tissue samples. in this study, a taqman(®) real-time pcr assay was designed based on the phosphoprotein gene of porv-lpmv, to allow specific amplifi ...201323305816
persistence of porcine rubulavirus in experimentally infected boars.porcine rubulavirus is the etiological agent of blue eye disease in pigs. in boars, this virus causes orchitis and epididymitis and reduces seminal quality. the objective of this study was to determine the persistence of porcine rubulavirus in experimentally infected boars. nine 12-month-old boars were infected with 5 ml of the pac-3 strain of porcine rubulavirus at 1 × 10(5) tcid(50)/ml and held for 142 days post infection (dpi) to evaluate humoral immune response. the virus was isolated in cel ...201323201243
genetic and antigenic changes in porcine rubulavirus.blue eye disease, caused by a porcine rubulavirus (porv), is an emergent viral swine disease that has been endemic in mexico since 1980. atypical outbreaks were detected in 1990 and 2003. growing and adult pigs presented neurological signs, mild neurological signs were observed in piglets, and severe reproductive problems were observed in adults. amino acid sequence comparisons and phylogenetic analysis of the hemagglutinin-neuraminidase (hn) protein revealed genetically different lineages. we u ...201222754092
transcription of interferon stimulated genes in response to porcine rubulavirus infection in vitro.porcine rubulavirus (porv) is an emerging virus causing meningo-encephalitis and reproductive failures in pigs. little is known about the pathogenesis and immune evasion of this virus; therefore research on the mechanisms underlying tissue damage during infection is essential. to explore these mechanisms, the effect of porv on the transcription of interferon (ifn) pathway members was analyzed in vitro by semi-quantitative rt-pcr. ten tcid50 of porv stimulated transcription of ifnα, ifnβ, stat1, ...201124031738
neuraminidase activity of blue eye disease porcine rubulavirus: specificity, affinity and inhibition studies.porcine rubulavirus (porpv), also known as la piedad michoacan virus (lpmv) causes encephalitis and reproductive failure in newborn and adult pigs, respectively. the hemagglutinin-neuraminidase (hn) glycoprotein is the most exposed and antigenic of the virus proteins. hn plays central roles in porpv infection; i.e., it recognizes sialic acid-containing cell receptors that mediate virus attachment and penetration; in addition, its neuraminidase (sialic acid releasing) activity has been proposed a ...201728502901
[atomospheric pollens in ciudad de la piedad, mich]. 19675610196
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