PMID(sorted ascending)
chromosomal evolution of the chinese muntjac (muntiacus reevesi).the aim of this study was to test the validity of the hypothesis that the 2n=46 karyotype of the chinese muntjac (muntiacus reevesi) could have evolved through 12 tandem fusions from a 2n=70 hypothetical ancestral karyotype, which is still retained in chinese water deer (hydropotes inermis) and brown-brocket deer (mazama gouazoubira). combining fluorescence-activated chromosomal sorting and degenerate oligonucleotide-primed polymerase chain reaction, we generated chromosome-specific dna paint pr ...19979169585
defining the anatomy of the rangifer tarandus sex chromosomes.a comprehensive cytogenetic characterization of the unusally large reindeer (rangifer tarandus) sex chromosomes is presented for the purpose of studying the evolution of these atypical gonosomes. sex chromosome idiograms were constructed from g-banded and c-banded chromosomes to illustrate the relative amounts and locations of euchromatin and heterochromatin. hybridization with a mazama gouazoubira x whole-chromosome paint revealed that essentially all reindeer x-linked euchromatin and most rein ...19989567201
"osmetrichia" in the grey brocket deer (mazama gouazoubira).osmetrichia have been defined as hairs specialized in the storage of secretions used in olfactory communication between conspecifics (müller-schwarze, et al. 1977). these authors found highly specialized osmetrichia in the tarsal gland tufts of black-tailed but not white-tailed deer. chemical communication appears to be well developed in grey brocket deer: the bucks scent mark by rubbing their foreheads on bushes, and all deer urinate and defecate almost exclusively on dung heaps. brocket deer a ...199910904542
ticks associated with wild animals in the nhecolândia pantanal, brazil.a study of ticks associated with wild animals was carried out from september 1996 to april 1998 at the fazenda alegria (21,000 ha), in the nhecolândia pantanal, state of mato grosso do sul, brazil, a sunken plain bordering the upper paraguay river, located 19 x 08's; 56 x 46'w. a total of 81 wild animals (13 species, 6 orders) were captured with the aid of nets, and ticks were found on 63 (78%). tick species identified included boophilus microplus (canestrini), amblyomma cajennense (f.), a. parv ...200011193635
outbreak of malignant catarrhal fever in brown brocket deer (mazama gouazoubira) in brazil. 200212233830
ticks (acari: ixodidae) on wild animals from the porto-primavera hydroelectric power station area, brazil.from june 2000 to june 2001, a total of 741 ticks were collected from 51 free-living wild animals captured at the porto-primavera hydroelectric power station area, located alongside an approximately 180 km course of the paran river, between the states of s o paulo and mato grosso do sul, comprising 9 species of 3 genera: ambly-omma (7 species), boophilus (1) and anocentor (1). a total of 421 immature amblyomma ticks were reared in laboratory until the adult stage, allowing identification of the ...200312563479
disease survey of free-ranging grey brocket deer (mazama gouazoubira) in the gran chaco, bolivia.samples from 17 free-ranging hunter-killed grey brocket deer (mazama gouazoubira) in the gran chaco, bolivia, were collected during june-august 1999. all 17 deer appeared to be in good condition at the time of death. gross necropsies were performed, serum was collected for serologic evaluation of selected infectious disease agents, and feces and ectoparasites were collected for evaluation of internal and external parasites. serologic tests were positive for antibodies against bovine respiratory ...200415137493
monitoring ovarian cycles and pregnancy in brown brocket deer (mazama gouazoubira) by measurement of fecal progesterone the present study, pregnancy and the estrous cycle were monitored in captive brown brocket deer (mazama gouazoubira) by measuring fecal progestagens with a commercial enzyme immunoassay (eia), along with behavioral data. fecal samples were collected twice a week during pregnancy and daily during the estrous cycle and post-partum period. it was possible to distinguish between inter-luteal and luteal phases of the estrous cycle. behavioral estrus corresponded with low concentrations of fecal pr ...200615979135
mitochondrial and nuclear phylogenies of cervidae (mammalia, ruminantia): systematics, morphology, and biogeography.the family cervidae includes 40 species of deer distributed throughout the northern hemisphere, as well as in south america and southeast asia. here, we examine the phylogeny of this family by analyzing two mitochondrial protein-coding genes and two nuclear introns for 25 species of deer representing most of the taxonomic diversity of the family. our results provide strong support for intergeneric relationships. to reconcile taxonomy and phylogeny, we propose a new classification where the famil ...200616584894
sex-related macrostructural organization of the deer's brachial plexus.we describe the morphological organization of the deer brachial plexus in order to supply data to veterinary neuroclinics and anaesthesiology. the deer (mazama gouazoubira) brachial plexus is composed of four roots: three cervical (c6, c7 and c8) and one thoracic (t1). within each sex group, no variations are observed between the left and the right brachial plexus, though sex-related differences are seen especially in its origin. the origin of axillary and radial nerves was: c6, c7, c8 and t1 in ...200717617108
ophthalmic patterns of captive brown brocket deer (mazama gouazoubira).captive brown brocket deer (mazama gouazoubira) were manually restrained to assess tear production by the schirmer tear test i to measure intraocular pressure by applanation tonometry, to examine ocular conjunctival epithelial cells via cytologic and histologic samples, and to survey ocular conjunctival microflora by microbiologic culture. the mean value for the schirmer tear test i was 8.9 +/- 1.8 mm/min, and the mean intraocular pressure was 15.3 +/- 3.1 mm hg. conjunctival epithelium containe ...200718229857
[phylogenetic relationships among neotropical deer genera (artiodactyla: cervidae) by means of dnamt sequences and microsatellite markers].the current work shows two molecular phylogenetic analyses on neotropical deers. in the first analysis, the mitochondrial control region (d-loop) was sequenced in six odocoileinae species from latin america, using the sequences of two muntiacinae as outgroups. the results obtained were as follows: a sequence of mazama americana showed a striking relationship with several sequences of odocoileus in contrast to that expected, since this m. americana haplotype, from a mexican origin, was not associ ...200719069784
comparison of two methods of synchronization of estrus in brown brocket deer (mazama gouazoubira).this study aimed to establish a protocol for synchronization of estrus in brown brocket deer (mazama gouazoubira). two groups of hinds (n=3) were submitted to two different protocols: treatment 1 received an intravaginal progesterone (cidr) device for 8 days, followed by 265microg injection of cloprostenol at the time of removal; and treatment 2 received two injections of 265microg of cloprostenol 11 days apart. after 30 days, each group of three hinds received the other treatment. treatment eff ...201019501991
carotid artery exteriorization in brown brocket deer (mazama gouazoubira) for an experimental study of anesthesia.this report evaluates the carotid artery exteriorization technique to allow repeated percutaneous artery catheterization in six brown brocket deer (mazama gouazoubira). repeated percutaneous catheterization of the carotid artery was possible for periods of 3 mo to obtain arterial blood and monitor arterial blood pressure of deer without risk of arterial rupture. the artery pulse was easily palpable for periods up to 15 mo. postoperative complication and/or arterial damage was not observed.200919746862
hosts, distribution and genetic divergence (16s rdna) of amblyomma dubitatum (acari: ixodidae).we supply information about hosts and distribution of amblyomma dubitatum. in addition, we carry out an analysis of genetic divergence among specimens of a. dubitatum from different localities and with respect to other neotropical amblyomma species, using sequences of 16s rdna gene. although specimens of a. dubitatum were collected on several mammal species as cattle horse, tapirus terrestris, mazama gouazoubira, tayassu pecari, sus scrofa, cerdocyon thous, myocastor coypus, allouata caraya, glo ...201020084537
reconstruction of the putative cervidae ancestral karyotype by chromosome painting of siberian roe deer (capreolus pygargus) with dromedary probes.the siberian roe deer (capreolus pygargus) is one of a few deer species presumably preserving the ancestral cervid karyotype. the comparative genomic data of the siberian roe deer are critical for our understanding of the karyotypic relationships within artiodactyls. we have established chromosomal homologies between the siberian roe deer and the dromedary (camelus dromedarius) by cross-species chromosome painting with dromedary chromosome-specific painting probes. dromedary chromosome paints de ...201020413959
helminthological screening of free-ranging grey brocket deer mazama gouazoubira fischer, 1817 (cervidae: odocoileini) from brazilian pantanal wetlands, with considerations on pygarginema verrucosa (molin, 1860) kadenatzii, 1948 (spirocercidae: ascaropsinae).the brazilian pantanal wetlands are a unique ecosystem with a faunistic composition similar to that of the adjacent biomes but in higher densities. the early establishment of domestic cattle husbandry in that area introduced pathogens that may influence the indigenous pantanalian fauna. in this paper, the authors describe the helminthfauna of ten free-ranging adult mazama gouazoubira from the paiaguás sub-region, along with the descriptors of infection. morphological description of pygarginema v ...201020549069
different responses to doxorubicin-induced chromosome aberrations in brazilian deer species.the tendency toward chromosome fragility is one of the theories that may explain chromosome variation in brocket deer species (genus mazama). we tested doxorubicin as an inducer of chromosome aberrations in lymphocytes of three brocket deer species, mazama gouazoubira, m. americana and m. nana, compared to the marsh deer, blastocerus dichotomus. doxorubicin, at a concentration of 0.25 microg/ml, induced chromosome aberrations and fragile sites in all four species; the highest frequencies were se ...201020714996
effects of physical and chemical immobilization on hematologic and biochemical variables in captive brown brocket deer (mazama gouazoubira).alterations in cellular and chemical constituents in blood are indicators of physical or chemical restraint in deer.201021039715
detection of theileria and babesia in brown brocket deer (mazama gouazoubira) and marsh deer (blastocerus dichotomus) in the state of minas gerais, brazil.intraerythrocytic protozoan species of the genera theileria and babesia are known to infect both wild and domestic animals, and both are transmitted by hard-ticks of the family ixodidae. the prevalences of hemoprotozoa and ectoparasites in 15 free-living mazama gouazoubira, two captive m. gouazoubira and four captive blastocerus dichotomus from the state of minas gerais, brazil, have been determined through the examination of blood smears and the use of nested polymerase chain reaction (npcr). t ...201021354704
anatomical description of arterial segments of the spleen of deer.with 2 figures201121426378
do precipitation and food affect the reproduction of brown brocket deer mazama gouazoubira in conditions of semi-captivity?the births of brown brocket deer ( mazama gouazoubira) in a secondary lower montane forest called "yunga" in northwestern argentina were compared with rainfall. analyses were performed with rainfall and flower-fruit fall in an attempt to determine the possible importance of these seasonal variables in birthing. the births were not directly correlated with rainfall, but rather with the flower and fruit fall of exotic plant species. this may be related to favor the development of fawns, which eat ...201021562691
polyspecific associations between squirrel monkeys (saimiri sciureus) and other primates in eastern of the most common types of polyspecific association observed in neotropical primate communities is that between squirrel monkeys (saimiri) and capuchins (cebus). the present study focused on association patterns in two saimiri sciureus groups in eastern brazilian amazonia, between march and october, 2009. the associations were analyzed in terms of the species involved, the degree of association, and niche breadth and overlap. the study involved two s. sciureus groups (b4 and gi) on the righ ...201121809365
histomorphometric evaluation of the neotropical brown brocket deer mazama gouazoubira testis, with an emphasis on cell population indexes of spermatogenic yield.information on the reproductive biology of neotropical cervids is scarce. therefore, the aim of this study was to perform biometric, histologic and stereologic analyses of the brown brocket deer mazama gouazoubira testis, with an emphasis on the intrinsic yield and the sertoli cell index. seven adult males kept in captivity were used. the animals were immobilized; anesthetized and testicle fragments were obtained by biopsy incision. the material was fixed, processed and examined by routine histo ...201121889273
ovarian mucinous cystadenoma in a gray brocket deer (mazama gouazoupira).an ovarian mucinous cystadenoma is described in a gray brocket deer (mazama gouazoupira). the tumor was histologically characterized by the presence of cysts and proliferation of papillae, both lined by single- or multi-layered pleomorphic epithelial cells that contained alcian blue-positive mucins.201121908297
comparison of three protocols for superovulation of brown brocket deer (mazama gouazoubira).this study aims to evaluate the ovulation rate and the presence of functional corpora lutea after treatment by three different protocols designed to cause superovulation in brown brocket deer. six female received an intravaginal device containing 0.33 g of progesterone (cidr®) for 8 days, followed by 0.5 mg injection of estradiol benzoate at the time of insertion and 265 µg of cloprostenol at the time of removal. afterwards, the hinds were divided into three groups (n = 2): treatment a received ...201322057947
molecular detection of tick-borne pathogens of the family anaplasmataceae in brazilian brown brocket deer (mazama gouazoubira, fischer, 1814) and marsh deer (blastocerus dichotomus, illiger, 1815).deer are important natural reservoir hosts of anaplasmataceae. the present study used nested pcr and nucleotide sequencing to evaluate the occurrence of anaplasmataceae species in 23 free-living and six captive specimens of the cervids mazama gouazoubira and blastocerus dichotomus in minas gerais state, brazil. blood samples were tested for the presence of ehrlichia and anaplasma spp. using npcr assays and sequencing of the msp4, msp1 and 16s rrna genes. the identity of each sequence was confirm ...201122136597
natural infection by endoparasites among free-living wild animals.the objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living wild animals. fecal samples from wild mammals and birds at rehabilitation centers in the states of mato grosso do sul and são paulo were analyzed by sedimentation and flotation-centrifugation methods. parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. cryptosporidium sp. oocysts were detected in fecal samples from oncillas (leopardus ti ...201323778826
morphological examination of the obturator notch and canal in cervidae.the purpose of this study was to investigate gross findings of the obturator notch (on) and obturator canal (oc) in cervidae. a total of 183 pelvic girdles from 26 species of deer were examined, and the obturator canal (oc) was classified into 4 types based on the degree of separation from the obturator foramen (of). the deep on was observed primarily in the subfamily capreolinae (telemetacarpal deer). the small bony oc was frequently observed in hydropotes inermis, mazama gouazoubira and ozotoc ...201424430657
physiologic effects of three different protocols of isoflurane anesthesia in captive brown brocket deer (mazama gouazoubira).this study evaluates the cardiorespiratory and physiologic effects of three different protocols of chemical restraint using isoflurane in captive brown brocket deer (mazama gouazoubira). six adult deer, two males and four females, aged 3-6 yr old and weighing 16.3 +/- 1.5 kg (mean +/- sd), were used. the deer were physically restrained and anesthetized using one of three protocols: protocol 1 (p1), anesthesia was induced directly with isoflurane using a facemask and maintained for 1 hr with isof ...201324450047
arterial thoracic vascularization in some deer species: pampas deer (ozotoceros bezoarticus), brown brocket deer (mazama gouazoubira) and axis deer (axis axis).in this study, the arterial distributions of the aortic arches of three deer species (axis axis, ozotoceros bezoarticus and mazama gouazoubira) were described. the animals were dissected immediately after being found dead. latex injection method was used to observe the vascularization of the thorax. the branching pattern of the arteries of the thoracic aorta in o. bezoarticus was similar to domestic ruminants. in the m. gouazoubira and a. axis, there were no bicarotid trunk. interestingly, the f ...201424611999
comparing two different superovulation protocols on ovarian activity and fecal glucocorticoid levels in the brown brocket deer (mazama gouazoubira).stress is a limiting factor in assisted reproduction in wild animals maintained in captivity. however, the knowledge of assisted reproduction techniques for wild animals is useful for future in situ and ex situ conservation programs. thus, this study evaluated the ovulation rate, presence of functional corpora lutea and fecal glucocorticoid levels following treatments promoting superovulation in captive brown brocket deer.201424646096
use of filter papers to determine seroprevalence of toxoplasma gondii among hunted ungulates in remote peruvian amazon.toxoplasmosis is a zoonosis caused by the protozoan toxoplasma gondii, and it is found worldwide. to determine whether ungulates are reservoirs of t. gondii in an isolated and remote region of the northeastern peruvian amazon, antibodies to t. gondii were determined in 5 species of ungulates by the modified agglutination test (mat). these animals were hunted by subsistence hunters along the yavarí-mirín river, in the northeastern peruvian amazon. blood samples were collected by hunters on filter ...201424918073
post-mortem hemoparasite detection in free-living brazilian brown brocket deer (mazama gouazoubira, fischer 1814).tick-borne infections can result in serious health problems for wild ruminants, and some of these infectious agents can be considered zoonosis. the aim of the present study was the post-mortem detection of hemoparasites in free-living mazama gouazoubira from minas gerais state, brazil. the deer samples consisted of free-living m. gouazoubira (n = 9) individuals that died after capture. necropsy examinations of the carcasses were performed to search for macroscopic alterations. organ samples were ...201425054500
characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer mazama gouazoubira.the complete mitochondrial genome of the brown brocket deer mazama gouazoubira and a set of polymorphic microsatellite markers were identified by 454-pyrosequencing. de novo genome assembly recovered 98% of the mitochondrial genome with a mean coverage of 9-fold. the mitogenome consisted of 16,356 base pairs that included 13 protein-coding genes, two ribosomal subunit genes, 22 transfer rnas and the control region, as found in other deer. the genetic divergence between the mitogenome described h ...201726500438
medium- and large-sized mammals in a steppic savanna area of the brazilian pampa: survey and conservation issues of a poorly known fauna.the wildlife of the brazilian pampa is threatened by large-scale habitat loss, due in particular to the expansion of soybean cultivation and the conversion of grasslands areas into extensive areas of silviculture. it is essential to study how the mammal fauna copes with the highly fragmented, human-influenced, non-protected landscape. our study presents the results of a survey of the large- and medium-sized mammals of a typical human-influenced steppic savanna area of the pampa biome. the survey ...201626840582
medium- and large-sized mammals in a steppic savanna area of the brazilian pampa: survey and conservation issues of a poorly known fauna.the wildlife of the brazilian pampa is threatened by large-scale habitat loss, due in particular to the expansion of soybean cultivation and the conversion of grasslands areas into extensive areas of silviculture. it is essential to study how the mammal fauna copes with the highly fragmented, human-influenced, non-protected landscape. our study presents the results of a survey of the large- and medium-sized mammals of a typical human-influenced steppic savanna area of the pampa biome. the survey ...201626909626
contrasting origin of b chromosomes in two cervids (siberian roe deer and grey brocket deer) unravelled by chromosome-specific dna sequencing.b chromosomes are dispensable and variable karyotypic elements found in some species of animals, plants and fungi. they often originate from duplications and translocations of host genomic regions or result from hybridization. in most species, little is known about their dna content. here we perform high-throughput sequencing and analysis of b chromosomes of roe deer and brocket deer, the only representatives of cetartiodactyla known to have b chromosomes.201627516089
isolation and attempted cultivation of an anaplasma marginale strain from brazilian brown brocket deer (mazama gouazoubira, fisher, 1814) in the tick cell line ide8.the aim of the study was to isolate and establish an anaplasma marginale strain from brazilian brown brocket deer, mazama gouazoubira, in the ixodes scapularis cell line ide8. blood from a free-living adult female m. gouazoubira naturally infected with a. marginale (mgi5) was inoculated intravenously into a splenectomized calf. when a. marginale rickettsemia was 2.5%, blood was collected and cryopreserved in liquid nitrogen with dimethylsulfoxide (dmso). ide8 cell cultures were infected with cal ...201627612925
fauna used by rural communities surrounding the protected area of chapada do araripe, brazil.studies on the inter-relations between people and animals have been considered essential to better understand the dynamics of socio-ecological systems. this study aimed to register the animal species known by the communities adjacent to national forest of araripe, their uses and if the close relationship affects the knowledge of useful species.201627642747
molecular characterization of anaplasma sp. in free-living gray brockets (mazama gouazoubira).bacteria in the genus anaplasma are responsible for diseases in animals and humans. studies carried out in brazil have demonstrated that brazilian deer are able to act as hosts of agents in the family anaplasmataceae and are possibly potential reservoirs of these pathogens. molecular and phylogenetic studies have been carried out on samples of two gray brocket specimens (mazama gouazoubira) from the city of guarapuava, state of paraná, brazil, for the detection of anaplasma sp. in these animals. ...201727992293
using sperm morphometry and multivariate analysis to differentiate species of gray mazama.there is genetic evidence that the two species of brazilian gray mazama, mazama gouazoubira and mazama nemorivaga, belong to different genera. this study identified significant differences that separated them into distinct groups, based on characteristics of the spermatozoa and ejaculate of both species. the characteristics that most clearly differentiated between the species were ejaculate colour, white for m. gouazoubira and reddish for m. nemorivaga, and sperm head dimensions. multivariate an ...201628018612
are brazilian cervids at risk of prion diseases?prion diseases are neurodegenerative fatal disorders that affect human and non-human mammals. chronic wasting disease (cwd) is a prion disease of cervids regarded as a public health problem in north america, and polymorphisms at specific codons in the prnp gene are associated with this disease. to assess the potential cwd susceptibility of south american free-ranging deer, the presence of these polymorphisms was examined in mazama gouazoubira, ozotoceros bezoarticus and blastocerus dichotomus. d ...201728281927
identifying mazama gouazoubira (artiodactyla; cervidae) chromosomes involved in rearrangements induced by doxorubicin.the process of karyotype evolution in cervidae from a common ancestor (2n = 70, fn = 70) has been marked by complex chromosomal rearrangements. this ancestral karyotype has been retained by the current species mazama gouazoubira (fischer 1814), for which a chromosomal polymorphism (robertsonian translocations and the presence of b chromosomes) has been described, presumably caused by a chromosome fragility. thus, this study has identified doxorubicin-induced chromosome aberrations and mapped the ...201728590504
detection of bartonella sp. in deer louse flies (lipoptena mazamae) on gray brocket deer (mazama gouazoubira) in the neotropics.louse flies or deer keds, lipoptena spp., are widespread in neotropical cervids, but the vector-borne pathogens of louse flies had only been previously reported in the northern hemisphere. this is the first report of bartonella spp. in deer louse flies (lipoptena mazamae) in the neotropics collected from gray brocket deer ( mazama gouazoubira ) in southern brazil. dna from bartonella sp. was detected in all 429 l. mazamae collected from 11 road-killed gray brocket deer. the same sequences of dna ...201728749268
the effects of cryopreservation on different passages of fibroblast cell culture in brown brocket deer (mazama gouazoubira).the brown brocket deer mazama gouazoubira is 1 of the 10 recognized brocket deer of the neotropical region. recently, this species has suffered a population decline due to current threats, mainly poaching and habitat loss. several studies have shown that some endangered species can benefit from interspecies somatic cell nuclear transfer technology through the use of their somatic cells, such as the fibroblasts. thus, the aim of this study was to verify the viability and the effect of cryopreserv ...201728922611
karyotype relationships among selected deer species and cattle revealed by bovine fish probes.the cervidae family comprises more than fifty species divided into three subfamilies: capreolinae, cervinae and hydropotinae. a characteristic attribute for the species included in this family is the great karyotype diversity, with the chromosomal numbers ranging from 2n = 6 observed in female muntiacus muntjak vaginalis to 2n = 70 found in mazama gouazoubira as a result of numerous robertsonian and tandem fusions. this work reports chromosomal homologies between cattle (bos taurus, 2n = 60) and ...201729112970
activity pattern of medium and large sized mammals and density estimates of cuniculus paca (rodentia: cuniculidae) in the brazilian pampa.between july 2014 and april 2015, we conducted weekly inventories of the circadian activity patterns of mammals in passo novo locality, municipality of alegrete, southern brazil. the vegetation is comprised by a grassy-woody steppe (grassland). we used two camera traps alternately located on one of four 1 km transects, each separated by 1 km. we classified the activity pattern of species by the percentage of photographic records taken in each daily period. we identify cuniculus paca individuals ...201829489928
intrapopulation chromosomal polymorphism in mazama gouazoubira (cetartiodactyla; cervidae): the emergence of a new species?mazama gouazoubira is a small deer species widely distributed in south america. previous studies have shown that this species presents intraspecific chromosomal polymorphisms, which could affect fertility due to the effects of chromosomal rearrangements on gamete formation. important aspects regarding the karyotype evolution of this species and the genus remain undefined due to the lack of information concerning the causes of this chromosomal variation. nineteen individuals belonging to the maza ...201829656282
a retrospective pathology study of two neotropical deer species (1995-2015), brazil: marsh deer (blastocerus dichotomus) and brown brocket deer (mazama gouazoubira).this retrospective study describes the biological and epidemiological aspects, gross and microscopical findings, and most likely causes of death (cd) in two species of neotropical deer in brazil. the animals were collected between 1995 and 2015 and represented 75 marsh deer (md) and 136 brown brocket deer (bbd). summarized, pneumonia was diagnosed microscopically in 48 md and 52 bbd; 76 deer suffered trauma, involving dog attack (14 bbd) and vehicle-collision (14 bbd). pulmonary edema (50 md; 55 ...201829879222
molecular survey of rickettsia spp. in the neotropical deer tick haemaphysalis juxtakochi from brazilian pampa.spotted fever (sf) is a tick-borne disease associated with rickettsia spp.. in the pampa biome, southern brazil, cases of sf seem to be strongly linked with the practice of hunting wild animals. an investigation of rickettsiae in tick species found on wild animals could provide more information regarding the rickettsiosis enzootic cycle. the aim of this study is to describe the results of a molecular survey of rickettsia spp. in the neotropical deer tick, haemaphysalis juxtakochi cooley, 1946 (a ...201829980888
variability, morphometrics, and co-variation of the os lacrimale in ruminantia, the lacrimal bone forms a considerable part of the facial skeleton, and the morphology of its facial facet is highly variable when compared to other mammals. in this study, we quantify the species-specific variability in size and shape of the lacrimal facial facet in species of cervidae (deer) and relate it to systematics and various aspects of their ecology and behavior. we sampled 143 skull specimens from 10 genera; 12 moschus and 3 tragulus specimens were used as outgroups. we ...201931095779
evidence of exposure to coxiella burnetii in neotropical free-living cervids in south america.coxiella burnetii (order legionellales, family coxiellaceae), the etiological agent of q fever, is a pleomorphic, obligate gram-negative intracellular bacillococcus that can infect humans and animals. among the mammals hosting this agent, both domestic and wild ruminants are of particular economic and public health importance. ticks and other hematophagous arthropods or aerosols are incriminated in the transmission between reservoirs and susceptible hosts. this study used serological and molecul ...201931128095
non-homogeneous distribution of steroids in fecal pellets: an example in brown brocket deer (mazama gouazoubira) with progesterone metabolites.measuring reproductive hormones in feces has become an important tool in the endocrine characterization of wild animals' reproduction. however, several factors may influence its success, such as fecal collection and storage techniques, knowledge of steroid hormone metabolism, the extraction procedure, immunoassay selection, inherent factors, and the distribution of steroid hormones in the feces. it is known that the distribution of these hormones in the feces is not homogeneous, and prior to the ...201931201799
development and evaluation of a duplex taqman qpcr assay for detection and quantification of trypanosoma cruzi infection in domestic and sylvatic reservoir hosts.a question of epidemiological relevance in chagas disease studies is to understand trypanosoma cruzi transmission cycles and trace the origins of (re)emerging cases in areas under vector or disease surveillance. conventional parasitological methods lack sensitivity whereas molecular approaches can fill in this gap, provided that an adequate sample can be collected and processed and a nucleic acid amplification method can be developed and standardized. we developed a duplex qpcr assay for accurat ...201931783770
protein profile of the ovarian follicular fluid of brown brocket deer (mazama gouazoubira; fisher, 1814).the aim of this study was to characterize the protein profile of ovarian follicular fluid (ff) of brown brocket deer (mazama gouazoubira). five adult females received an ovarian stimulation treatment and the ff was collected by laparoscopy from small/medium (≤3.5 mm) and large (>3.5 mm) follicles. concentrations of soluble proteins in ff samples were measured and proteins were analyzed by 1-d sds-page followed by tryptic digestion and tandem mass spectrometry. data from protein list defined afte ...201931787122
new records and genetic diversity of mycoplasma ovis in free-ranging deer in brazil.cervids represent a mammal group which plays an important role in the maintenance of ecological balance. recent studies have highlighted the role of these species as reservoirs for several arthropods-borne pathogens. globally, hemotropic mycoplasmas (haemoplasmas) are emerging or remerging bacteria that attach to red blood cells of several mammals species causing hemolytic anaemia. therefore, the aim of this study was to investigate the occurrence and assess the phylogenetic positioning of mycop ...202031933451
in vitro development and mitochondrial gene expression in brown brocket deer (mazama gouazoubira) embryos obtained by interspecific somatic cell nuclear transfer.the genetic diversity of neotropical deer is increasingly jeopardized, owing to declining population size. thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. the transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. accordingly, the aim of this study was to produce brown brocket deer embryos by interspecific somatic cell nuclear transfer (iscnt), using goat or cattle oocytes ...202032559409
detection of a putative novel genotype of anaplasma in gray-brocket deer (mazama gouazoubira) from uruguay.anaplasmataceae includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia, comprising a group of obligate intracellular bacteria. the genus anaplasma has pathogenic species transmitted by ticks of veterinary and human health importance. wild ungulates such as deer represent important reservoirs and amplifiers of anaplasmataceae. the interaction between deer and domestic ruminants represents a serious problem due to the transmission of these pathogens through their ectoparasites. in t ...202032647904
characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (mazama gouazoubira, fischer, 1814).populations of gray brocket deer (mazama gouazoubira) are declining; yet, knowledge on the reproductive biology of this species remains limited. therefore, this study aimed to describe morphology, viability, membrane integrity, mitochondrial activity, morphometry, micromorphology, and ultrastructure of the gray brocket deer sperm. three adult male gray brocket deer were used in the study. semen collection was performed using electroejaculation. semen were analyzed by evaluating ph, motilities, v ...202032959457
cryptosporidium parvum in brown brocket (mazama gouazoubira) from brazil: first report of the subtype iiaa16g3r1 in cervids.this research had as objective to evaluate the occurrence and to characterize genetically the infections by cryptosporidium in mazama gouazoubira. by a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six brazilian localities. the cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp), using the mitoch ...202033137502
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