| sequence analysis of rna-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species. | the complete nucleotide sequence of rna-2 from a japanese isolate ip of broad bean wilt virus (bbwv) was determined. the sequence encodes a single large polyprotein, which contains a putative movement protein and two coat proteins (cps). the 3'-terminal sequences of rna-2 were also determined for three other japanese isolates and two atcc isolates (pv132 and pv176) of bbwv. the cps of the four japanese isolates share 86.8-98.0% amino acid sequences homology with one another and 88.3-96.5% with t ... | 1999 | 10481749 |
| the complete sequence of a spanish isolate of broad bean wilt virus 1 (bbwv-1) reveals a high variability and conserved motifs in the genus fabavirus. | the genome of a spanish isolate of broad bean wilt virus-1 (bbwv-1) was completely sequenced and compared with available sequences of other isolates of the genus fabavirus (bbwv-1 and bbwv-2). this consisted of two rnas of 5814 and 3431 nucleotides, respectively, and their organization was similar to that of other members of the family comoviridae. its mean nucleotide identity with a bbwv-1 american isolate was 81.5%, and between 59.8 and 63.5% with seven bbwv-2 isolates. our analysis showed seq ... | 2005 | 15986170 |
| broad bean wilt virus causes necrotic symptoms and generates defective rnas in capsicum annuum. | abstract a virus was isolated from hot pepper (capsicum annuum cv. hyang chon) growing in korea and displaying necrotic spots or streaks on leaves and stems followed by stunting and death of plants. morphological and host range analyses of extracts from infected plants suggested that the causal agent of disease was a broad bean wilt virus (bbwv), and the virus was tentatively named a korean isolate of bbwv (bbwv-k). when the isolate was back-inoculated onto hot pepper plants, it induced symptoms ... | 2000 | 18943381 |
| gentian mosaic virus: a new species in the genus fabavirus. | abstract a viral isolate, designated n-1 and obtained from a gentian (gentiana scabra) plant that exhibited mosaic symptoms, was transmitted mechanically to nine plant species in six families. these plants are known as hosts of fabaviruses. the n-1 isolate was composed of isometric particles 30 nm in diameter and included two rna molecules of approximately 6.0 and 3.6 kb in length, as estimated by agarose gel electrophoresis. the rnas were encapsidated separately in two of the three types of par ... | 2005 | 18943990 |
| detection and identification of species of the genus fabavirus by rt-pcr with a single pair of primers. | the genus fabavirus includes three species: broad bean wilt virus 1 (bbwv-1), bbwv-2 and lamium mild mosaic virus (lmmv), but a new candidate species, gentian mosaic virus (gemv), has been proposed. analysis of the complete nucleotide sequences of fabaviruses was used to design a pair of conserved primers for specific detection of members of this genus. these primers encompassed the 5'-terminal non-translatable region (ntr) , whose size for bbwv-1, bbwv-2 and gemv was different. rt-pcr, with thi ... | 2007 | 17475342 |
| occurrence of viruses infecting pea in iran. | a survey was conducted to determine the incidence of alfalfa mosaic virus (amv), bean yellow mosaic virus (bymv), broad bean wilt virus-1 (bbwv), pea leafroll virus (plrv), pea enation mosaic virus (pemv), pea seed borne mosaic virus (psbmv), potato virus x(pvx), tomato mosaic virus (tomv), tomato spotted wilt virus (tswv) on pea (pisum sativum) in iran. a total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in tehran province of iran, whe ... | 2006 | 17390891 |
| new molecular methods for identification of broad bean wilt virus 1. | broad bean wilt virus 1 (bbwv-1) causes damages in economically important plant crops such as pepper, bean, spinach, etc. fast, cheap and reliable diagnostic tools are crucial to limit or control the disease. in this work, tissue blot immunoassay (tbia), dot-blot (db) and tissue-print (tp)-hybridization were developed for bbwv-1 diagnosis and evaluated for sensitivity, specificity and reliability in plants of several host species grown in the greenhouse or in the field, in comparison with elisa ... | 2008 | 18417319 |
| genetic variability and evolution of broad bean wilt virus 1: role of recombination, selection and gene flow. | analysis of four genomic regions from 37 geographically diverse isolates of broad bean wilt virus 1 (bbwv-1) showed high genetic diversity in comparison to most plant viruses. comparison of synonymous and nonsynonymous substitutions of the small coat protein gene (scp) revealed negative selection for most amino acid positions. phylogenetic analysis of scp showed that some bbwv-1 isolates from distant geographical areas were genetically close, suggesting long-distance migration. analysis of genet ... | 2014 | 24122109 |
| detection and identification of fabavirus species by one-step rt-pcr and multiplex rt-pcr. | the genus fabavirus of the family secoviridae comprises a group of poorly characterized viruses. to date, only five species have been described: broad bean wilt virus 1 (bbwv-1), broad bean wilt virus 2 (bbwv-2), lamium mild mosaic virus (lmmv), gentian mosaic virus (gemv) and cucurbit mild mosaic virus (cummv). the development is described of two rt-pcr procedures for the detection and identification of fabavirus species: a one-step rt-pcr using a single pair of conserved primers for the detect ... | 2014 | 24361876 |
| molecular and biological characterization of highly infectious transcripts from full-length cdna clones of broad bean wilt virus 1. | broad bean wilt virus 1 (bbwv-1), genus fabavirus, has a genome composed of two single-stranded positive-sense rnas of ∼5.8 (rna1) and 3.4kb (rna2). full-length cdna clones of both genomic rnas (pbenr1 and pbenr2) from bbwv-1 isolate ben were constructed under the control of the t7 promoter. in vitro derived capped transcripts were infectious in nicotiana benthamiana, chenopodium quinoa and vicia faba plants. the biological activity of viral transcripts was not affected by extra bases at the 5'- ... | 2016 | 26951858 |
| detection and absolute quantitation of broad bean wilt virus 1 (bbwv-1) and bbwv-2 by real time rt-pcr. | broad bean wilt virus 1 (bbwv-1) and bbwv-2 are the two most significant viruses in the genus fabavirus, causing damage to many economically important agricultural crops worldwide. a quantitative real-time reverse transcription-polymerase chain reaction (rt-qpcr) procedure using two taqman(®)mgb probes was developed for sensitive and specific detection and quantitation of bbwv-1 and bbwv-2. primers and probes were designed from conserved sequence stretches to detect all isolates of each virus. s ... | 2011 | 21855578 |
| antipredator behaviour of myzus persicae affects transmission efficiency of broad bean wilt virus 1. | biological control has the potential to limit the population growth of arthropod vectors and consequently may be expected to reduce plant virus spread in a crop. however, reduction of vector abundance is not the only effect of biological control. natural enemies might induce antipredator behaviour that affects feeding and dispersal of vectors, and therefore virus spread. here we test the effect of two natural enemies on dispersal of the aphid vector myzus persicae and transmission of broad bean ... | 2011 | 21549771 |
| genome sequence and characterization of a new virus infecting mikania micrantha h.b.k. | the complete rna genomic sequence of a new virus infecting mikania micrantha, designated as mikania micrantha wilt virus (mmwv), has been determined. the genomic sequence and the predicted gene products of mmwv were similar to those of the other viruses of the genus fabavirus. the mmwv nucleotide sequence showed 75.6% identity to that of gentian mosaic virus, 56.6 and 57% identity to those of two broad bean wilt virus 1 isolates, and between 55.7 and 58% identity to those of seven broad bean wil ... | 2008 | 18679764 |