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epidemiological and pathological studies on congenital diffuse hyperplastic goiter in calves.perinatal diseases such as weak calf, stillbirth, or abortion were found in 86 calves from about 600 cows in a farm (a farm) in 1987 and 1988. such perinatal diseases were decreased to 36 calves in 1989 and 1990 after an additional supply of seaweed was made to the maternal feeds. from these cases, 23 stillborn and weak calves were pathologically examined. the mean weight with standard deviation of the 18 thyroid glands was 36.3 +/- 28.6 g before feeding of the seaweed supplement, and 12 (67%) o ...19911790233
[somatic hybrids between rorippa indica (linn.) hiern and brassica napus l. through protoplast-fusion system].high yield of protoplast isolation was achieved from hypocotyls of b. napus l. and leaves of rorippa indica (linn.) hiern. the isolated protoplasts were used to establish an efficient protoplast-fusion system between the two cruciferous species by peg-dmso method and culturing with ms liquid medium. ten somatic fusion hybrids between b. napus and r. indica were obtained. the enzyme combinations for isolating protoplast from b. napus l. and r. indica were 1% cellulase + 0.2% macerozyme + 3 mmol/l ...200717650493
functional characterization of rorippa indica defensin and its efficacy against lipaphis erysimi.rorippa indica, a wild crucifer, has been previously reported as the first identified plant in the germplasm of brassicaceae known to be tolerant towards the mustard aphid lipaphis erysimi kaltenbach. we herein report the full-length cloning, expression, purification and characterization of a novel r. indica defensin (rid) and its efficacy against l. erysimi. structural analysis through homology modeling of rid showed longer α-helix and 3rd β-sheet as compared to brassica juncea defensin (bjd). ...201627186475
glucosinolate diversity within a phylogenetic framework of the tribe cardamineae (brassicaceae) unraveled with hplc-ms/ms and nmr-based analytical distinction of 70 desulfoglucosinolates.as a basis for future investigations of evolutionary trajectories and biosynthetic mechanisms underlying variations in glucosinolate structures, we screened members of the crucifer tribe cardamineae by hplc-ms/ms, isolated and identified glucosinolates by nmr, searched the literature for previous data for the tribe, and collected hplc-ms/ms data for nearly all glucosinolates known from the tribe as well as some related structures (70 in total). this is a considerable proportion of the approximat ...201627743600
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