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multiplex rt-pcr detection of four aphid-borne strawberry viruses in fragaria spp. in combination with a plant mrna specific internal control.the principal aphid-borne viruses infecting strawberry (fragaria spp.) strawberry crinkle virus (scv), strawberry mild yellow edge virus (smyev), strawberry mottle virus (smov) and strawberry vein banding virus (svbv) can cause serious crop losses. in this paper, a multiplex reverse transcriptase polymerase chain reaction (rt-pcr) method is described for the simultaneous detection of all four viruses in combination with a plant mrna specific internal control which can be used as an indicator of ...200312880923
strawberry vein banding virus detection by double antibody sandwich elisa with antibodies to recombinant coat protein. 200212387506
preferential banding of secondary veins in strawberry is caused by mixed virus infection.leaves of fragaria ananassa duch. cv. redgauntlet with mottle and mild dwarf symptoms were grafted onto f. vesca indicator clones. the youngest leaves developed specific vein banding pattern located preferentially on secondary veins near the edge of the leaves. electron microscopy of ultrathin sections and negatively stained purified virus preparations from symptom-bearing strawberry leaves revealed presence of different-sized isometric virions. particles of about 50 nm and 23 nm in diameter wer ...199910825923
strawberry vein banding virus--definitive member of the genus caulimovirus.the complete dna sequence (7876 nucleotides) of strawberry vein banding virus (svbv) has been determined. seven open reading frames are detected that potentially code for proteins of calculated weight 37.8; 18.3; 16.6; 56.0; 81.1; 59.0 and 12.6 kda, respectively. their position on the viral genome is the same as that of the corresponding proteins on the cauliflower mosaic virus (camv) genome. phylogenetic analysis based on the amino acid sequence of this protein shows a closer relationship of sv ...19989654684
diagnosis of strawberry vein banding virus by a non-radioactive probe.a non-radioactive digoxigenin-labelled cdna probe was prepared from genomic dna of american isolate no. 45058 of strawberry vein banding virus (svbv). five different air-dried svbv-containing strawberry leaf samples originating from national clonal germplasm repository, corvallis, usa, reacted positively in dot blot hybridization with this probe. six of twelve strawberry samples from the czech republic exhibiting symptoms of svbv-like infection gave positive reaction with this probe. our results ...19968891093
quarantine strawberry vein banding virus firstly detected in slovakia and serbia.strawberry vein banding virus (svbv) was detected by polymerase chain reaction (pcr) and dot-blot hybridisation in samples of cultivated strawberry plants originating from central slovakia and in samples of wild strawberry plants from south-eastern serbia in federal republic of yugoslavia (fry). this is the first finding of svbv in these countries as well as of svbv in wild strawberry plants in europe.19989770076
expression and genomic integration of transgenes after agrobacterium-mediated transformation of mature barley embryos.mature embryos in tissue cultures are advantageous because of their abundance and rapid germination, which reduces genomic instability problems. in this study, 2-day-old isolated mature barley embryos were infected with 2 agrobacterium hypervirulent strains (agl1 and eha105), followed by a 3-day period of co-cultivation in the presence of l-cystein amino acid. chimeric expression of the b-glucuronidase gene (gusa) directed by a viral promoter of strawberry vein banding virus was observed in cole ...201525730049
transcriptome analysis of woodland strawberry (fragaria vesca) response to the infection by strawberry vein banding virus (svbv).woodland strawberry (fragaria vesca) infected with strawberry vein banding virus (svbv) exhibits chlorotic symptoms along the leaf veins. however, little is known about the molecular mechanism of strawberry disease caused by svbv.201627411713
complete nucleotide sequence of strawberry vein banding virus chinese isolate and infectivity of its full-length dna clone.strawberry vein banding virus (svbv) is a double-stranded dna plant virus, which has been found in north america, australia, brazil, japan, europe and several provinces of china. infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins. it is one of the most economically important diseases in asiatic, european and north american strawberry-growing areas.201627716385
strawberry vein banding virus isolates in eastern canada are molecularly divergent from other isolates.the complete sequence of a strawberry vein banding virus (svbv) isolate collected in nova scotia, canada, and designated ns8, was determined. the 7,856-nucleotide circular double-stranded dna genome contains seven open-reading frames (orfs), which is consistent with other svbv isolates and other members of the genus caulimovirus. comparison of ns8 with other whole-genome sequences retrieved from databases revealed that ns8 shares the highest sequence similarity (96.5% identity) with isolate chin ...201728190195
detection of strawberry vein banding virus by polymerase chain reaction and dot blot hybridization.strawberry vein banding virus (svbv) is one of seventeen members of the family caulimoviridae. natural infection with the virus is known in fragaria species only. infections caused by svbv are often symptomless (1), but their significance increases in mixed infections with strawberry crinkle or strawberry latent c viruses (2,3). this virus has been originally found on strawberries in usa and firstly described by frazier (4), but it is probably world-wide distributed by planting or breeding mater ...19979391655
use of conserved genomic regions and degenerate primers in a pcr-based assay for the detection of members of the genus caulimovirus.the genus caulimovirus consists of several distinct virus species with a double-stranded dna genome that infect diverse plant species. a comparative analysis of the sequences of known caulimovirus species revealed two regions that are conserved in all caulimovirus species with the exception of strawberry vein banding virus. degenerate primers based on these two regions were designed and tested in a polymerase chain reaction-based assay for broad spectrum detection of members of this genus. cauli ...200919100290
evaluation of constitutive viral promoters in transgenic soybean roots and nodules.the efficiency of beta-glucuronidase (gus) expression was evaluated with five viral promoters to identify the most suitable promoter or promoters for use in soybean hairy roots, including applications to study the symbiotic interaction with bradyrhizobium japonicum. levels of gus activity were fluorimetrically and histochemically assayed when the gus (uida) gene was driven by the cauliflower mosaic virus (camv) 35s promoter and enhanced 35s (e35s) promoter, the cassava vein mosaic virus (csvmv) ...200818616399
infectivity of recombinant strawberry vein banding virus dna.infectivity of the cloned dna genome of strawberry vein banding virus (svbv) was demonstrated by particle bombardment of 4-week-old strawberry (fragaria vesca l. var. uc-5) plants with gold particles coated with the putative full-length 7.9 kb viral dna. vein banding symptoms developed on 15 % of inoculated plants 6-7 weeks post-inoculation. an approximate 1.25-mer of the viral dna was cloned into the binary vector pcgn1547. particle bombardment of this construct into strawberry plants gave an i ...200312771404
identification of a novel plant virus promoter using a potyvirus infectious clone.a putative promoter from the strawberry vein banding caulimovirus (svbv) genome was identified by its ability to drive infection with full-length cdna of the zucchini yellow mosaic rna potyvirus (zymv). a high rate of infection was obtained with the cdna under control of the svbv promoter using particle bombardment technology. the svbv promoter shows 60% homology to the cauliflower mosaic virus 35s promoter in the domain spanning the conserved motifs of ccact (at -83) and the tata box (at -31), ...200010766302
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