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some factors controlling the attachment of the rumen holotrich protozoa isotricha intestinalis and i. prostoma to plant particles in vitro.the rumen holotrich protozoa isotricha intestinalis and i. prostoma showed chemotaxis to sucrose, glucose and fructose. they attached themselves, by means of an organelle on the anterior cell surface, to particulate sources of these carbohydrates provided soluble protein was present in the medium. the concentration of protein eliciting attachment varied with the species and the state of nutrition of the cell, but was between 20 and 150 microgram ml-1. attachment occurred only if the concentratio ...1978418148
incorporation of 14c-labelled components of escherichia coli and of amino acids by isotricha intestinalis and isotricha prostoma from the sheep rumen. 19674965675
hydrogenosomes in a mixed isolate of isotricha prostoma and isotricha intestinalis from ovine rumen contents.1. both isotricha intestinalis and i. prostoma possess microbody-like organelles, with a highly granular appearance. 2. these organelles, which are sedimentable at 10(5) g-min, bear no morphological similarity to mitochondria, but are enzymatically similar to organelles possessed by certain other anaerobic protozoa and termed hydrogenosomes. 3. the hydrogenosomes isolated from a preparation of mixed isotrichs bear a closer similarity to those isolated from the other rumen holotrich. dasytricha r ...19836403285
ruminal fermentation and duodenal flow following progressive inoculations of fauna-free wethers with major individual species of ciliate protozoa or total fauna.naturally fauna-free (ff) wethers, equipped with ruminal and duodenal cannulas, were used in two groups of eight (group a) and seven (group b) animals in six consecutive experimental periods, each lasting for 28 d. the objective was to measure ruminal fermentation traits, and flows of nonammonia nitrogen (nan), total amino acid (taa), and bacterial nitrogen (bn) from the stomach after inoculation with individual ciliate protozoa species in each period. the wethers in both groups were fed a diet ...200010764084
the rumen ciliate fauna of domestic sheep (ovis ammon aires) from the turkish republic of northern cyprus.concentration and composition of ciliate protozoa in the families ophryoscolecidae and isotrichidae were determined in rumen contents of domestic sheep (ovis ammon aries) from cyprus. a total of five genera of ophryoscolecidae were identified, metadinium, enoploplastron, polyplastron, epidinium, and ophrvoscolex, which included six species: metadinium affine, enoploplastron triloricatum, polyplastron multivesiculatum, epidinium ecaudatum, epidinium graini, and ophryoscolex purkynjei. eight separ ...200111456322
lipid metabolism of rumen ciliates and bacteria. ii. uptake of fatty acids and lipid analysis of isotricha intestinalis and rumen bacteria with further information on entodinium simplex.the total lipid and free fatty acid contents of isotricha intestinalis, entodinium simplex, and the rumen bacterial flora of the respective protozoa were determined. warburg manometric data showed that the sodium salts of tributyrin, oleic, and acetic acids stimulated gas production in i. intestinalis, whereas tributyrin was stimulatory with e. simplex and less active with oleic and acetic acids. rumen bacteria provided fatty acids produced lower manometric gaseous increases when compared with t ...196314000915
potential of galvanotaxis to separation and cleaning of rumen ciliates.the ability of rumen ciliate protozoa to move in a unidirectional electrical field from the anode to the cathode was tested in large-volume electromigration equipment; the aim was to concentrate the microorganisms and clean them of impurities. during galvanotaxis in the freshly harvested rumen fluid and at a voltage of 10 v (i=0.8 ma), cells of isotricha (isotricha prostoma, isotricha intestinalis) were the first to swim towards the cathode; 1 min later, they were followed by dasytricha ruminant ...200415003689
horizontal gene transfer from bacteria to rumen ciliates indicates adaptation to their anaerobic, carbohydrates-rich environment.the horizontal transfer of expressed genes from bacteria into ciliates which live in close contact with each other in the rumen (the foregut of ruminants) was studied using ciliate expressed sequence tags (ests). more than 4000 ests were sequenced from representatives of the two major groups of rumen cilates: the order entodiniomorphida (entodinium simplex, entodinium caudatum, eudiplodinium maggii, metadinium medium, diploplastron affine, polyplastron multivesiculatum and epidinium ecaudatum) a ...200616472398
methane production and substrate degradation by rumen microbial communities containing single protozoal species in vitro.to assess the effect of protozoal species on rumen fermentation characteristics in vitro.200717944841
assessment of protozoa in yunnan yellow cattle rumen based on the 18s rrna sequences.library of ruminal protozoal 18s rrna of yunnan yellow cattle has been constructed in the present study. phylogenic analysis of sequences was meanwhile employed to reveal the diversity of protozoa in the rumen of yunnan yellow cattle. one yellow cattle was fed malt meal (ycrpb) and the other was fed wheat straw (ycrps). a protozoa-specific primer (p-ssu-342f) and a eukarya-specific primer (medlin b) were used to amplify a 1,360-bp fragment of dna encoding protozoal small subunit (ssu) ribosomal ...201120358294
detection of methanogens and proteobacteria from a single cell of rumen ciliate protozoa.rumen ciliate-associated bacteria and methanogenic archaea were analyzed by a 16s rrna gene retrieved from a single cell of polyplastron multivesiculatum, isotricha intestinalis, and ophryoscolex purkynjei. rumen fluid was taken from a ruminally fistulated goat to prepare a ciliate fraction. ciliate mixtures were incubated under mixtures of antibiotics for 48 h to eliminate extracellular bacteria. individual cells of rumen ciliates were selected under microscopic observation after fixation with ...200415754246
isotricha jalaludinii n. sp. found from the rumen of lesser mouse deer, tragulus javanicus, in malaysia.isotricha jalaludinii n. sp. found in the rumen of lesser mouse deer, tragulus javanicus, in malaysia was described and illustrated. this new species is characterized by the location and direction of the vestibulum, shape of the macronucleus, and absence of a dent at the vestibular opening. the presence of single peculiar isotrichid species in the rumen of mouse deer, which is recognized as one of the most primitive ruminants, suggests that the isotrichid ciliates similar to i. jalaludinii and i ...19957728142
[ultrastructure and taxonomic position of the infusorian isotricha intestinalis from the rumen of ruminants]. 19816795776
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