Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
immunodiagnostic moieties in somatic and excretory/secretory antigens of fasciola gigantica.immunodiagnostic potential fractions in fluke antigens and excretory/secretory (e/s) antigen of f. gigantica have been identified using double immunodiffusion (did), immunoelectrophoresis (iep) and sodium dodecyl sulphate (sds) page electrophoresis. hyperimmunised rabbits elicited considerable level of antibody response with few precipitin lines showing reaction of identity in did suggesting presence of some common fractions in all antigens. mature fluke somatic (mfsag) and immature fluke somati ...19958786154
cytochemical localization of cytochrome c oxidase activity in mitochondria in the tegument and tegumental and parenchymal cells of the trematodes echinostoma trivolvis, zygocotyle lunata, schistosoma mansoni, fasciola gigantica and paragonimus ohirai.cytochrome c oxidase in the mitochondria of the tegument and tegumental and parenchymal cells was examined cytochemically in echinostoma trivolvis, zygocotyle lunata, schistosoma mansoni, fasciola gigantica and paragonimus ohirai, trematodes that inhabit different sites in their vertebrate hosts. clear differences in enzyme activity occurred in the mitochondria of these species, probably reflecting the different energy metabolisms of these worms. marked aerobic metabolism occurred in s. mansoni ...19958522764
evaluation of fasciola excretory-secretory (e/s) product in diagnosis of acute human fasciolosis by igm elisa.diagnosis of fasciolosis in the acute phase depends on a sensitive and accurate serological test. the present study is an evaluation of the efficacy of excretory-secretory fasciola gigantica adult worm antigen by igm elisa. thirty eight patients with acute fasciolosis and 14 in the chronic phase together with 23 patients with different parasitic infections were introduced in the study. seventeen healthy, parasite free individuals, were served as controls. a crude excretory-secretory antigen and ...19958525282
helminth parasites and hypobiosis of nematodes in n'dama cattle during the dry season in the gambia.three series of necropsies of cattle were performed, corresponding to early dry season, approximately 1 month after the last rains (november, n = 6), mid dry season (february, n = 6) and end dry season (april, n = 3). eggs per gram of faeces (epg) were determined just before necropsy. three trematodes (fasciola gigantica, schistosoma spp. and paramphistomatids) and 11 nematodes were identified from cattle, with the prevalence rate varying from 6.7% to 100%. haemonchus contortus was the most abun ...19958644452
detection of circulating antigen in sera of fasciola gigantica-infected cattle with antibodies reactive with a fasciola-specific 88-kda antigen.antibodies against a specific 88-kda antigen of fasciola gigantica were used for the detection of circulating antigen in the sera of cattle with experimental and natural infections of f. gigantica by a double antibody elisa. circulating antigen was detectable as early as the second and third weeks after infection and positive absorbance values were obtained for the entire duration of infection. absorbance values decreased below the cutoff point 3 weeks after chemotherapy with oxyclozanide. this ...19957571328
time-course analysis of antibody response by eitb and elisa before and after chemotherapy in sheep infected with fasciola gigantica.the time-course analysis of the antibody response to fascicola gigantica infection in sheep was studied by enzyme-linked immunosorbent assay (elisa) and enzyme-linked immunoelectrotransfer blot (eitb). sera from sheep experimentally infected with f. gigantica were reacted with excretory-secretory antigens of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in anti-fasciola antibody by 2 weeks after infection and there was a sharp decrease in an ...19957571329
separation of outer cysts from metacercariae of fasciola gigantica in an aqueous two-phase system.the outer cysts (oc) of fasciola gigantica metacercariae (mc) were separated in an aqueous two-phase system composed of polyethylene glycol and dextran in distilled water. a large number of separated mc without oc were recovered in the bottom layer rich in dextran in the system. ten separated mc in the layer were used to inoculate the mouse with a syringe and juveniles were recovered from the animals on the 14th day. when the number of juveniles recovered was compared with that from the mice inf ...19957622796
immunodiagnosis of fasciolosis in ruminants using a 28-kda cysteine protease of fasciola gigantica adult worms.a homogeneous extract of a 28-kda cysteine protease of fasciola gigantica adult worms was used as the antigen for immunodiagnosis of fasciolosis in cattle, sheep and goats using the falcon assay screening test enzyme-linked immunosorbent assay technique. this antiprotease assay technique was found to be very rapid and sensitive and may be useful as a supplementary method for the diagnosis of fasciolosis in ruminants.19957660568
development and characterization of a monoclonal antibody reactive with a 28 kda protease of fasciola gigantica.a monoclonal antibody that was reactive with a 28-kda protease of fasciola gigantica was produced after hyperimmunizing balb/c mice with a partially purified extract and a purified 28-kda protease obtained from the fluke. isotype analysis showed that the immunoglobulin is igg3. seven other monoclonals of isotypes igg1, igg2 and igg3 which reacted with the protease and one to three other protein bands were also produced. in addition, four other clones of hybridoma produced antibodies which recogn ...19957660572
[epidemiology of gastrointestinal helminthiasis in small ruminants in the tree-cropping pasture zone in senegal].an epidemiological survey on gastrointestinal helminthiasis in 51 sheep and 51 goats was held in the tree-cropping pasture region in senegal from october 1990 to september 1991. all the animals examined were infected with at least 1 helminth species. three trematodes (fasciola gigantica, schistosoma bovis, amphistomatids), 2 cestodes (moniezia expansa, cysticercus tenuicollis) and 9 nematodes were identified. the most important parasite in sheep was haemonchus contortus while trichostrongylus co ...19957735304
biomphalaria alexandrina naturally infected with fasciola gigantica in egypt. 19957747303
partial characterization of larval antigens of strongyloides papillosus by western blot analysis.western blotting was used to analyse the antigens in extracts of adult and infective larvae (sil) of strongyloides papillosus with serum of infected rabbits. several proteins in both the extracts reacted with immunoglobulin g in the serum. a protein of about 16 kda in the extract of sil showed an especially strong reaction. no cross-reaction was observed among proteins in the extracts of adult worms of such cattle helminths as fasciola gigantica, homalogaster paloniae, trichuris discolor and set ...19947812315
molecular weight determination of fasciola antigens specific for diagnosis of acute fascioliasis.acute human fascioliasis depends in its diagnosis on detecting specific antibodies in the sera of patients. it was observed that both somatic fraction i and crude e/s products of adult fasciola gigantica worm gave sensitive and specific results. using igm elisa, the cut off o.d was 0.6 and 0.3 nm. and the accuracy was 93% and 98% for the two antigens respectively. determination of the molecular weight of these two antigens was undertaken by sds-page. the molecular weight of the somatic fraction ...19947844411
serodifferentiation of human fascioliasis from schistosomiasis.a dot enzyme-linked immunosorbent assay (dot-elisa) was developed using excretory-secretory (es) antigens of fasciola gigantica adult worms and freeze-thaw antigen (aft) of adult schistosoma mansoni worms. specific igg and igm antibodies were simultaneously investigated in sera from 17 febrile eosinophilic hospitalized patients and 20 healthy controls. dot-elisa was shown useful and could differentiate fascioliasis from schistosomiasis prior to parasite egg detection in patient's stool. this ass ...19947855923
efficacy of triclabendazole against fasciola gigantica in buffaloes in eastern nepal. 19947886900
[immunodiagnosis of fasciola gigantica fascioliasis by elisa technique in senegal. preliminary observations in 2 lambs].the purpose of this work was to investigate the use of the elisa test for the diagnosis of fasciolosis (fasciola gigantica) in sheep, in senegal, using fasciola gigantica metabolic excretion-secretion products as antigens. coprological analysis results were negative until the fourteenth week following infection; using the elisa test, the first anti-fasciola gigantica antibodies can be detected as early as the fourth week following infection.19947709030
several observations concerning cercarial sheddings of fasciola gigantica from lymnaea natalensis.the shedding of cercariae from lymnaea natalensis and their transformation into metacercariae occurred mainly at night, and 70.7% of the cysts were counted between 7 p.m. and 1 a.m. there is no infradian rhythm in the numerical distribution of the daily mean values as related to experiment duration or patent period duration. one quarter of the snails shed their cercariae in a single wave and the others in 2 to 13 waves. floating cysts represent 35% of the total number of metacercariae and their ...19949235192
distribution of antigens of fasciola gigantica at various stages of development.an indirect immunofluorescent antibody test on the serum of a fasciola gigantica-infected rabbit, showed specific fluorescence on the tegumental surface of miracidia, sporocysts, rediae, cercariae, metacercariae and juvenile worms aged between one and seven weeks. the same reaction occurred in the gut cells of cercariae and metacercariae and in the gut caeca of juveniles in the liver. the results of the absorption test indicated that stage-specific antigen(s) in metacercariae and seven-week-old ...19938460268
comparison of the second internal transcribed spacer (ribosomal dna) from populations and species of fasciolidae (digenea).the nucleotide sequence of the 3' end of the second internal transcribed spacer region (its2) was determined for four isolates of fasciola hepatica, two isolates of fasciola gigantica, one isolate of fascioloides magna and one isolate of fasciola sp. from japan. intraspecific sequence divergence was negligible. sequence divergence between f. hepatica and f. gigantica was 2.8% and between fasciola hepatica and fascioloides magna was 13.2%. the sequence of fasciola sp. from japan matched closely t ...19938359995
effects of ration and fasciola infection on some blood parameters of ewes.this study was conducted using fourty cross-bred ewes in 5 groups of which one was kept as a control and the others were infected with metacercariae of fasciola gigantica twice at the beginning of the experiment and 200 days from the first. the four infected groups fed on basal ration or high protein ration with or without mineral mixture. blood samples were taken from the five groups to assess the concentrations of plasma fibrinogen and serum total proteins, albumin, globulin, calcium and inorg ...19938308350
cloning and sequence analysis of the major cysteine protease expressed in the trematode parasite fasciola sp.a cdna encoding the fasciola cysteine protease precursor has been cloned and sequenced. the deduced precursor contains 325 amino acid residues (m(r) 36,768) consisting of a signal sequence (pre-region, 15 residues), pro-region (90 residues), and mature protease (220 residues, m(r) 24,371). cys27, his164, and asn184 form the catalytic triad in the active site of the mature enzyme, and their adjacent regions are highly conserved. the parasite enzyme showed 50, 44, and 27% amino acid sequence homol ...19938118429
liver fluke infections in livestock in borno state, nigeria.a survey was conducted on the prevalence of fasciola gigantica and dicrocoelium hospes eggs in gall bladders of cattle and small ruminants (sheep and goats) slaughtered between november 1987 and december 1988 in maiduguri, borno state, nigeria. prevalence rates of 42.7% and 5.7% were recorded for f. gigantica and d. hospes eggs, respectively, in 246 cattle and 718 sheep and goats examined. out of the 246 cattle examined, 52 (21.1%), 45 (18.3%) and 8 (3.3%) excreted eggs of f. gigantica, d. hospe ...19938122358
schistosomiasis and intestinal parasites in rural villages in southwest nigeria: an indication for expanded programme on drug distribution and integrated control programme in nigeria.a cross sectional survey of schistosomiasis, intestinal parasite infections and pattern of schistosomiasis transmission was undertaken in four rural villages around the erinle dam, nigeria. the prevalence of schistosoma haematobium ranged from 10% to 60%. 30% of infected school children excreted over 50 eggs/10 ml urine and high rates of haematuria, proteinuria, leucocyturia and nitrites in urine were observed in infected children and the villagers. prevalence of infection with s. mansoni ranged ...19938256092
the protective role of fasciola tegument antigen against trichinella spiralis infection.fasciola gigantica tegument antigen was prepared to be used for induction of immunity against trichinella spiralis in albino mice. the results of the study showed that the antigen was more effective when administered in a dose of 3 micrograms than in a dose of 30 micrograms. this was evidenced by the reduction in adult count 72%, adult length 61.4%, larval count 78.7%, length of muscle larvae 32.2% and the significant reduction in reproductive capacity index.19938376868
banding patterns of fasciola hepatica and fasciola gigantica (trematoda) by isoelectric focusing.liver flukes fasciola hepatica and fasciola gigantica are polymorphic and vary morphologically depending upon the host being parasitized. it is known also that mixed infection occurs where both species are present. a technique involving protein separation was used to distinguish the 2 species. isoelectric focusing of soluble proteins was performed on polyacrylamide gels using whole-body proteins from adult flukes. although many bands appeared common to both species and some were shared with host ...19938437051
larval stages of medically important flukes (trematoda) from vientiane province, laos. part ii. cercariae.the cercariae of five flukes, which are potential human parasites, opisthorchis viverrini (family opisthorchiidae), haplorchis taichui (heterophyidae), schistosoma spindale (schistosomatidae), fasciolopsis buski, and fasciola gigantica (fasciolidae) were found in freshwater snails from laos. the cercariae of the above species, recorded in laos for the first time, are illustrated and their morphology is briefly described. the snail helicorbis umbilicalis represents a new intermediate host of f. b ...19921290378
the purification and characterization of a cysteine protease of fasciola gigantica adult worms.a 26-28 kda protease was isolated from fasciola gigantica adult worms by a two-stage purification process of column chromatography in a sephacryl s-200 column and affinity chromatography in an l-phenylalanine-agarose column. this protease is a cysteine (thiol) proteinase with an optimum ph of 4.5 and is not inhibited by anti f. gigantica immunoglobulin g. the enzyme was inhibited by protease inhibitors known to inhibit cysteine proteases but not by metallo-, aspartate or serine protease inhibito ...19921413454
redial generations of fasciola gigantica in the pulmonate snail lymnaea truncatula.lymnaea truncatula, 4 mm in height, were subjected to infection by a single miracidium of fasciola gigantica, then raised at 23 degrees c until day 60 of the experiment. histological study of these snails demonstrated a mean redial burden of 34 parasites at day 60, of which one third were degenerating forms. the mean number of living independent rediae did not exceed 5 for the first and second generations. conversely, in subsequent generations there were as many as 18 rediae per snail at day 60. ...19921452990
seasonal prevalence of some zoonotic trematode infections in cattle and pigs in the north-east montane zone in india.seasonal variations in the prevalence of fasciola gigantica and eurytrema pancreaticum in cattle, and opisthorchis noverca, artyfechinostomum malayanum, fasciolopsis buski and gastrodiscoides hominis in pigs, were studied post-necropsy over a 1 year period in a humid, subtropical north-east hilly region in india. the overall prevalence rate was 53.02% in cattle (n = 960) and 12.92% in pigs (n = 960). fasciola gigantica and e. pancreaticum occurred throughout the year with peaks during cold winte ...19921561763
glycogen, protein and lipid content of adult fasciola gigantica (trematoda).the concentration of carbohydrates, proteins, and lipids in the whole worm, gut, and body musculature of fasciola gigantica was investigated from worms collected from cattle slaughtered in the jos abattoir. protein accounted for the highest concentration of the constituents constituting 62.59 +/- 0.86% of the dry weight of the fluke. this was followed by lipid with 28.15 +/- 0.86% of the dry weight while carbohydrate (glycogen) was 6.29 +/- 0.11% of the dry weight. thin-layer chromatography reve ...19921590595
cloning and characterization of ribosomal rna genes from opisthorchis viverrini.the ribosomal dna (rdna) unit of the liver fluke opisthorchis viverrini has been cloned and characterized. the results demonstrated that the total length of this unit is approximately 13 kb, containing 4.2 kb of large subunit (lsu) rdna, 2.0 kb of small subunit (ssu) rdna, 1.0 kb of transcribed spacer dna and 5.8 kb of non-transcribed + external transcribed spacer dna. examination of the non-transcribed spacer region between different rdna units showed variation in the restriction sites rather t ...19921594296
the effects of fascioliasis on the activities of some drug-metabolizing enzymes in desert sheep liver.desert sheep experimentally or naturally infected with fasciola gigantica were used to study the influence of infection on the activities of some drug-metabolizing enzymes found in the liver. the enzymes investigated were aminopyrine n-demethylase, aniline 4-hydroxylase and udp-glucuronyltransferase. the experimental infection was confirmed histologically by detection of fasciola eggs in faeces and by measuring the activities of sorbitol dehydrogenase (sd), glutamate dehydrogenase (gd) and aspar ...19921617399
control of fasciola gigantica with triclabendazole in indonesian cattle.the efficacy of triclabendazole (tcbz) against natural infections with fasciola gigantica was evaluated in a field study using 102 indonesian cattle. the animals were divided into two groups; one received tcbz at 12 mg/kg orally every eight weeks for one year while the other remained untreated. relative to controls the fasciola faecal egg count in treated animals was significantly reduced (p less than 0.01) to almost zero at all observations. there were no differences between the groups in blood ...19911686688
a purified fasciola gigantica worm antigen for the serodiagnosis of human fascioliasis.partially purified fasciola gigantica worm antigens were evaluated in an enzyme-linked immunosorbent assay (elisa) for the specific serological diagnosis of fascioliasis. the diagnostic potential of 10 antigen fractions derived from gel-filtration peaks (ii & iii) were evaluated individually or in combination against a panel of admission sera obtained from 50 patients subsequently diagnosed as having a variety of systemic parasitic diseases, and from 10 healthy volunteers with no recent history ...19911755065
[main features of helminth parasitism in cattle in ituri (haut-zaire). iii. geographic distribution and prevalence of the main helminths].a necropsic survey, carried out in eleven slaughterhouses in ituri (haut-zaïre), has permitted to define the geographic distribution and prevalences of the main cattle helminths. trematodes, except the paramphistomes, had a very heterogeneous distribution. prevalence of fasciola gigantica ranged from 9 to 72% according to the sites, but these variations were not linked to topographic or climatologic parameters. the infection with schistosoma bovis was much variable as well (12.5 to 72%) and seem ...19911775693
susceptibility to homologous reinfection with fasciola gigantica in goats.goats previously infected with 100 viable fasciola gigantica metacercariae and treated with rafoxanide (ranide, 7.5 mg/kg) at week 4 were not protected against subsequent homologous challenge with 250 metacercariae administered two weeks later. reinfection resulted in more severe hepatic lesions and a higher percentage of flukes recovered as compared with primarily infected controls. however, the size of flukes originating from the second (challenge) infection was considerably reduced. the plasm ...19911775694
comparative pathological study of hepatic changes induced by fasciola gigantica and gigantocotyle explanatum in javanese thin-tailed sheep.pathological changes in the liver induced by fasciola gigantica and gigantocotyle explanatum were readily distinguishable from each other. lesions associated with the migration of immature flukes through the parenchyma were a prominent feature of infection with fasciola gigantica, whereas lesions induced by gigantocotyle explanatum were confined to the large bile ducts. the size of the hepatic lesions increased during the course of infection with fasciola gigantica and was associated with the fo ...19911779036
partial purification and characterisation of the proteolytic enzymes of fasciola gigantica adult worms.the proteases of adult fasciola gigantica whole worm were analysed by preparative isoelectric focusing and by gelatin-substrate gel electrophoresis at acidic and neutral ph (4.5 and 7.0). at least 15 bands of proteases were observed. these proteases had molecular weights ranging from 26 to 193 kda and isoelectric points of 4.92-7.63. protease-rich fractions were subsequently separated from whole worm preparation of the parasite by filtration in sephacryl s-200. the proteases were able to digest ...19911788929
problem of fascioliasis in animal husbandry in thailand.one of the most important parasitic diseases in adult cattle and buffalo in thailand is fascioliasis, caused mainly by fasciola gigantica. the economic loss from fascioliasis in cattle and buffalo throughout thailand has been assessed at not less than 100 million baht. recent investigations have been shown that the average prevalence of f. gigantica in cattle and buffalo in thailand was 11.8%. however, the prevalence varies considerably between villages, ranging from 0 to 85%. the prevalence is ...19911822925
first report of infectious necrotic hepatitis (black disease) among nubian goats in sudan.in a flock of 425 female and male nubian goats in the khartoum province, an outbreak of a disease causing sudden death of 18 apparently healthy goats occurred (11 females and 7 males, 3-6 years old). adult fasciola gigantica were found in the livers of all goats and in seven of them cysticercus tenuicollis cysts. these organs showed necrotic and severe histopathological changes. clostridium novyi type b was isolated from necrotic areas of all livers and found to be highly pathogenic and toxigeni ...19911824134
common antigens of fasciola gigantica, dicrocoelium hospes and schistosoma bovis and their relevance to serology.the possession of common antigens by three trematode parasites which commonly occur together in ruminants in the tropics, fasciola gigantica, dicrocoelium hospes and schistosoma bovis was studied in relation to the reliability of serodiagnosis of infection with these helminths. the crude antigenic extracts of the three trematodes were subjected to sephacryl s-300 column chromatography and f. gigantica was fractionated into six peaks, s. bovis into nine peaks and d. hospes into seven peaks. commo ...19911909064
[does human distomiasis due to fasiola gigantica exist in the delta of the niger river in mali? (apropos of a serological survey)].finding of specific anti-fasciola antibodies in persons living in the central area of the delta of niger shows that human fascioliasis is a reality in mali. fasciola gigantica, the single species affecting livestock is probably the parasite concerned. we think that this parasitic disease in humans is underestimated, obscured by schistosomiasis which is hyperendemic in the country. complementary studies are much needed for better identification of the problem.19911943637
early diagnosis of human fascioliasis by the detection of copro-antigens using counterimmunoelectrophoresis.we have reported the use of partially purified fasciola gigantica worm antigens (peaks ii and iii separated by sephadex g-200) in counterimmunoelectrophoresis (ciep) for the specific and early detection of antibodies in sera of fascioliasis patients. in the present study, we report the use of hyperimmune serum from rabbits immunized with these partially purified worm antigens in ciep for the detection of parasite antigens in saline extracts of patients' stools. this test was conducted on stool s ...19911949144
characterization of the opisthorchis viverrini genome.the methylations of trematode genomic dna were analyzed using restriction enzymes and southern blot hybridization. restriction enzymes mspi, hpaii, hhai were used to probe cpg methylation while mboi, sau3a, dpni were used for a methylation. the results revealed that opisthorchis viverrini, fasciola gigantica and gigantocotyle siamensis had neither cpg nor a methylations. the presence of highly repeated dna elements was also demonstrated in o. viverrini genomic dna.19912050985
[inventory of fresh water snails in mayotte, comoro islands].no trematodosis is known at the present time in mayotte despite the close historical relationships between the mayotte archipelago and the neighbouring island of madagascar, which is heavily infested. it is noteworthy that no intermediate host has been described in previous studies in mayotte. an inventory of fresh water snails was performed from 1985 to 1988, in 53 experimental stations during the rainy and the dry seasons: 3,940 snails of eight different species were collected and further iden ...19902092350
triclabendazole in the treatment of fasciola gigantica infection in swamp buffalo (bubalus bubalis). 19902222372
causes of mortality in impala (aepyceros melampus) on 20 game farms in zimbabwe.the most common cause of death in impala (aepyceros melampus) herds on 20 game farms in zimbabwe was stress or physical injury during capture. acute fascioliasis due to fasciola gigantica was identified as the main disease problem associated with smaller game farms and where impala had been recently introduced. losses associated with ticks and tick-borne diseases were rare. impala in zimbabwe are maintained in a healthy condition on game farms where they are given supplementary feed during the d ...19902238405
countercurrent immunoelectrophoresis in the diagnosis of taenia hydatigena cysticercosis in goats.the procedure of countercurrent immunoelectrophoresis in the diagnosis of taenia hydatigena cysticercosis in goats was carried out for antemortem diagnosis of t. hydatigena cysticercosis in experimentally and naturally infected goats. the antigens of cyst fluid, scolex and membrane of t. hydatigena metacestodes were purified and compared. the sensitivity of the test in experimentally and naturally infected goats was 57.1 and 52.5%, respectively, whereas its specificity using antisera raised agai ...19902267724
comparative evaluation of the enzyme-linked immunosorbent assay (elisa) in the diagnosis of natural fasciola gigantica infection in cattle.the enzyme-linked immunosorbent assay (elisa) was evaluated for the diagnosis of naturally acquired fasciola gigantica infection in cattle in comparison with conventional parasitological techniques. using unfractionated whole worm extract of f. gigantica as the antigen, it was observed that there was a good correlation between elisa positivity and positive diagnosis of fascioliasis by post mortem liver examination, bile egg sedimentation and faecal egg sedimentation. there was, however, a dispar ...19902321350
antigens and antibodies in the ascitic fluid of mice infected with fasciola gigantica.fluke antigens were detected in the ascitic fluid of mice infected with fasciola gigantica. the precipitating antibodies reactive against the antigens from metacercariae were detected as early as 2 weeks after the infection, and those against the adult and immature worms appeared 3 and 4 weeks later.19902343528
[principal helminths of small ruminants in ituri (upper zaire)].from august 1986 to november 1987, about 180 slaughtered sheep and goats were examined at bunia (haut-zaïre) in order to establish the main helminth parasites. seventeen and fifteen species were identified in sheep and goats respectively. the occurrence of stilesia hepatica, oesophagostomum multifoliatum and muellerius capillaris as well as the absence of echinococcus granulosus constituted the original features of this check-list in the area. parasitism the most frequently encountered both in s ...19902369171
high resistance to experimental infection with fasciola gigantica in javanese thin-tailed sheep.innate resistance of javanese thin-tailed sheep to fasciola gigantica was investigated in animals infected with single doses of 150 or 500 metacercariae and killed 4, 8, 12 or 16 weeks after infection. infected and non-infected sheep had similar values for packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin concentration, serum glutamate dehydrogenase, serum gamma glutamyl transferase and serum aspartate transferase throughout the trial, except for one animal infected with ...19901979191
properties of phenol oxidase in fasciola gigantica.phenol oxidase of fasciola gigantica exists both as the soluble form as well as the membrane-bound form. the membrane-bound enzyme is considered to be a tyrosinase type because it is capable of oxidizing mono- and diphenol and is inefficient in oxidizing paraphenols. the soluble enzyme is a laccase type showing more affinity to various diphenols and paraphenols. membrane-bound enzyme exists as isoenzymes, showing 3 fractions, of which the slow-moving fraction is capable of oxidizing both 4-methy ...19892514408
restriction enzyme mapping of ribosomal dna can distinguish between fasciolid (liver fluke) species.recognition sites for nine different restriction endonucleases were mapped on rdna genes of fasciolid species. southern blots of digested dna from individual worms were probed sequentially with three different probes derived from rdna of schistosoma mansoni and known to span between them the entire rdna repeat unit in that species. eighteen recognition sites were mapped for fasciola hepatica, and seventeen for fasciola gigantica and fascioloides magna. each fasciolid species had no more than two ...19892552311
susceptibility of japanese lymnaea ollula to nigerian fasciola gigantica. 19892585940
specific detection of circulating surface/secreted glycoproteins of viable cysticerci in taenia saginata cysticercosis.a mouse monoclonal antibody (moab), reactive with a repetitive carbohydrate epitope on lentil-lectin adherent glycoproteins present on the surface and in the secretions of taenia saginata cysticerci, was used in the construction of a diagnostic elisa assay to detect these glycoproteins in the serum of t. saginata infected cattle. the moab was used as the trapping layer and subsequently bound glycoprotein was revealed using the same moab and biotinylated peroxidase-streptavidin complex as the dev ...19892674862
acute cholecystitis and fasciola sp. infection in thailand: report of two cases.two cases of fascioliasis gigantica from northeast thailand presenting with cholecystitis and gall stones were reported. both cases complained of abdominal pain. on laparotomy the worms were found. in one case five worms were recovered during bile duct exploration and bile drainage; eggs were also revealed in the bile. in the other case of ectopic fascioliasis one young adult worm was found in a nodule which adhered to liver and diaphragm. the parasites were identified as fasciola gigantica.19892699088
response of goats to repeated infections with fasciola gigantica.one or two mature primary infections with fasciola gigantica which had been removed by anthelmintic treatment resulted in a significant reduction in the number of flukes recovered from challenge infection as compared with that from controls. characteristic lesions of fascioliasis were seen in the livers of the 3 groups, however, goats with two primary abbreviated infections prior to challenge showed more severe lesions than those of animals with one primary abbreviated infection or those of chal ...19892728319
sensitivity of crude and purified fasciola antigens in immunological diagnosis of human fascioliasis.a study has been done to find the effect of purification of crude antigens, extracted from adult fasciola gigantica and fasciola hepatica worms on the sensitivity of some serological techniques in diagnosis of fascioliasis. using c.i.e.p. test, the partially purified antigens of both fasciola species were less sensitive than their corresponding crude ones. using i.h.a., the difference between results of crude and partially purified antigens for the two species were statistically insignificant, b ...19892768848
comparative study of sheep and buffalo isolates of fasciola gigantica in the intermediate host lymnaea auricularia.a comparison was made of the biological behaviour of two isolates of fasciola gigantica, obtained from sheep and buffalo, in the aquatic snail lymnaea auricularia. the sheep isolate exhibited a lower rate of infection, slower larval development and the production of fewer cercariae. larger eggs, miracidia and metacercariae were obtained from the buffalo isolate while the rediae and cercariae were smaller. metacercariae of sheep origin were dark brown with a mortality after 80 days of storage at ...19892799087
studies on the prevalence and laboratory transmission of fascioliasis in animals in the kashmir valley.in kashmir, 85.1% of cattle, 51.3% of sheep and 14.8% of goats were found infected with fasciola spp. the prevalence rate varied from 66.6 to 100.0%, 25.0 to 100% and nil to 66.0% in cattle, sheep and goats respectively in different months of the year. fasciola gigantica was the predominant species in all animal species but sheep harboured both f. gigantica and f. hepatica. the prevalence of f. hepatica infection in sheep happens to be the first report from india. lymnaea auricularia sensu stric ...19892920277
diacetyl reductase activity in the extracts of fasciola gigantica. 19883059781
fasciola in horses in the republic of south africa: a single natural case of fasciola hepatica and the failure to infest ten horses either with f. hepatica or fasciola gigantica.the faeces of 11 horses were examined for fasciola spp. eggs. one of them was positive for fasciola hepatica, a finding which was confirmed post-mortem. the 10 negative horses were subsequently infested with either f. hepatica or fasciola gigantica, each animal receiving orally from 500-9,500 metacercariae. no clinical signs were observed and no fluke eggs were detected in the faeces, and neither immature nor adult fasciola worms were recovered from the horses slaughtered 16-26 weeks post-infest ...19883194116
the effect of levamisole (l. tetramisole) treatment on the susceptibility to fasciola gigantica infection in goats. 19883201025
environmental stress and parasitic infections: i. the effects of gramoxone and hexadrin on embryo and hatching of fasciola gigantica miracidia.the effect of two chemical compounds commonly used in agriculture, gramoxone (a herbicide) and hexadrin (an insecticide) on embryonic development and hatching of fasciola gigantica miracidia were experimentally assessed. these two pesticides were introduced in varying quantities into petri dishes containing unembryonated eggs of the trematode for a period of 30 days. lc50 (lethal concentration for 50% hatching) values were determined for them. for gramoxone it was 2 ppm and for hexadrin it was 2 ...19883202331
[role of nitroxynil in the integrated control of fasciola gigantica fasciliasis in cattle in madagascar]. 19883217565
on the viability of fasciola gigantica metacercariae ingested by lymnaea auricularia.the effect of ingestion by lymnaea auricularia on the viability and infectivity of fasciola gigantica metacercariae was studied. the cyst wall was unaffected by the snail's digestive processes. two rabbits, each infected with 50 ingested metacercariae, died at 83 and 87 days post-infection. eight and 10 immature flukes were recovered from the livers, indicating that the metacercariae had remained infective after passing through the intestine of the snail.19883235797
environmental stress and parasitic infections: ii. effects of gramoxone and hexadrin (pesticides) on the survival characteristics of fasciola gigantica miracidia.miracidia of fasciola gigantica have a maximum longevity of 9 h. miracidial survival was found to be age dependent and the instantaneous mortality rate increased exponentially with miracidial age and pesticide treatment. mortality of the pesticide-treated miracidia differs significantly from that of the control. the lc50 value for hexadrin was calculated at 1.93 ppm and for gramoxone at 0.75 ppm. dosage-mortality lines when tested statistically show no significant difference between the two pest ...19883245635
fasciola gigantica: the second case of human infection in thailand. 19883251969
fasciola gigantica: pathological and helminthological observations in experimental infection of yankassa lambs. 19883253861
liver fluke infections of cattle in mali. an abattoir survey on prevalence and geographic distribution.six hundred and ninety two cattle, slaughtered in various abattoirs in mali between august 1983 and november 1984 were examined for evidence of fasciola gigantica and dicrocoelium hospes infections. a great variation in geographic prevalence was observed. sufficient knowledge was acquired to demonstrate both the importance of fascioliasis in some areas and also the high prevalence of d. hospes in other areas. we suggest that the difference in prevalence from one geographic area to another is inf ...19883400112
protective effect of irradiated metacercariae of fasciola gigantica and irradiated cercariae of schistosoma bovis against fascioliasis in goats.sensitisation of goats for eight weeks with metacercariae of fasciola gigantica gamma-irradiated at 3 kr resulted in significant resistance to an homologous challenge with normal metacercariae. however, serum sorbitol dehydrogenase assay suggested that, whereas little damage was produced by immunising infections, considerable hepatic damage was caused by flukes remaining from challenge infections. on the other hand sensitisation with cercariae of schistosoma bovis gamma-irradiated at 3 kr did no ...19873424453
studies on the energy reserve of fasciola gigantica (cobb.) from nigerian zebu cattle. ii. the fatty acid composition.the fatty acids were studied of the total lipid extracts of mature liver flukes fasciola gigantica from the bile ducts of nigerian zebu cattle. the high content of saturated fatty acids, which amounted to more than 54% of the total fatty acids, conferred a high level of saturation on these parasites. this was brought about by a general increase in the values of palmitic acid. the results are discussed in connection with the parasite-host relationship.19873426530
hepatic fascioliasis in the abattoirs of basrah.hepatic fascioliasis due to fasciola gigantica among slaughtered animals was studied at the basrah abattoirs from october 1984 to september 1985. the infection rates were 4.8, 3.3, 0.72 and 0.13% for buffaloes, cattle, sheep and goats respectively. the approximate annual loss due to condemnation of infected livers is estimated as 29,855 iraqi dinars.19873446027
serodiagnosis of fascioliasis in buffaloes by immunoprecipitation using adult fasciola gigantica antigens.partial purification of a saline extract of adult fasciola gigantica by chromatography on sephadex g200 gave four peaks, of which only the first two contained material which gave a precipitation reaction with sera from infected buffaloes. none of these gave false reactions with sera from uninfected animals but even the first and most sensitive fraction only reacted with 57.5 per cent of sera from animals known to be infected with moderate numbers of f gigantica.19873547527
fasciola gigantica in gallbladder. 19873583338
the protective effect of irradiated metacercariae of fasciola gigantica against homologous challenge in sheep.five lambs were each sensitized with 400 metacercariae of fasciola gigantica irradiated at 3 kr gamma rays. eight weeks later, they were each challenged together with 5 controls with 500 non-irradiated metacercariae. eight weeks later, all of the animals were necropsied and the worm burdens determined. the lambs which received the sensitizing infections had 80% less worms than the controls. the sensitized lambs also showed less hepatic damage compared with the controls as indicated by lower leve ...19873611708
[apropos of the origin of some diseases raging in madagascar].madagascar had been separated from african continent since 5 millions years and, if flora and fauna are endemic (40 species of lemuriens) all of parasites and humans populations are imported, from africa with respect to p. falciparum, p. malariae, s. mansoni and s. haematobium (but snails are endemic ), fasciola gigantica (imported with zebus) and perhaps p. vivax, and from asia with respect to w. bancrofti, n. americanus, c. cellulosae (imported with pigs) and perhaps p. vivax, chromoblastomyco ...19873326688
detection of fasciola gigantica eggs in faeces: a comparison of methods. 19873824857
serodiagnosis of fasciola gigantica infection in buffaloes.the agar gel precipitation test (agpt), counter immunoelectrophoresis (ciep) and indirect haemagglutination (iha) were evaluated for the diagnosis of fascioliasis due to fasciola gigantica in buffaloes. the sensitivity of these tests varied with the intensity of infection; and was greatest when the fluke burden in liver exceeded 100. ciep detected 76.06% of infected sera and was most sensitive, followed by iha which detected 68.37% of the infected sera. the agpt was found to be least sensitive, ...19873109111
differentiation of the rediae of fasciola hepatica and fasciola gigantica. 19863632105
studies on naturally-occurring ovine fascioliasis in the sudan.haematological, biochemical and pathological changes were investigated in 214 sheep naturally infected with fasciola gigantica in an endemic area in the sudan together with 82 uninfected controls. infected animals showed a clear decrease in erythrocyte counts, haemoglobin concentration and packed cell volume, a normochromic, normocytic anaemia, leucocytosis and eosinophilia. serum concentrations of the enzymes glutamate dehydrogenase, sorbitol dehydrogenase and glutamate oxaloacetic acid transam ...19863701020
studies on heterologous resistance between schistosoma bovis and fasciola gigantica in sudanese cattle.using the local strains of schistosoma bovis and fasciola gigantica, it was shown that sudanese zebu calves with mature primary infections of f. gigantica were highly resistant to challenge with s. bovis cercariae, and vice versa. liver enzyme tests showed that, in both cases, the primary infections had caused some liver damage. primary infection with irradiated s. bovis cercariae, which did not cause significant liver damage, did not protect significantly against challenge with f. gigantica.19863701021
some biochemical indices in naturally occurring fascioliasis in goats.some biochemical indices were assayed in goats naturally harbouring fasciola gigantica infection and compared with uninfected control goats. infected goats had significantly lower levels of serum glucose (47.6 +/- 1.8 mg dl-1) and albumin (3.1 +/- 0.1 g dl-1) and reduced albumin:globulin ratio (1.1 +/- 0.1). total lipid (526.8 +/- 2.4 mg dl-1), serum glutamate dehydrogenase (15.3 +/- 0.9 iu litre-1) and serum alkaline phosphatase (31.6 +/- 1.9 kind's and king's unit dl-1) were high in infected g ...19863704349
immunization of zebu calves against fasciola gigantica, using irradiated metacercariae.the pathogenesis of unirradiated, 3 krad-irradiated and 20 krad-irradiated metacercarial infections was compared in zebu calves studied over a 10-week period. calves exposed to 1000 unirradiated metacercariae (mc) became hypoalbuminaemic, and showed elevated serum concentrations of liver enzymes, whereas neither of the other groups was significantly affected. at slaughter, a mean of 332 flukes was recovered from the 0 krad group, while only 23% and 12% of this number were recovered from the 3 kr ...19863734396
experimental cholecystitis in goats caused by mature fasciola gigantica.mature fasciola gigantica obtained from naturally infected cattle were surgically transferred into the gallbladders of six fluke-free goats. two of the goats died at two and 19 weeks and the remaining four were slaughtered at 12, 48, 48 and 80 weeks after the transfer. nine of 20 transferred f gigantica were recovered from the gallbladder and common hepatic duct of the experimental goats. the walls of gallbladders and bile ducts were thickened by epithelial proliferation, fibrosis and mononuclea ...19854012032
studies on the energy reserve of fasciola gigantica from nigerian zebu cattle.as energy reserves in the body tissue of mature liver flukes var. fasciola gigantica from the bile ducts of nigerian zebu cattle the following percentages were found: protein 60.29, lipid 11.92, carbohydrate 7.0. the analysis of the lipid fractions showed the following composition: 47% phospholipids (63% phosphatidyl choline, 28% phosphatidyl serine, 9% lysophosphatidyl choline) and 22% triglycerides, 16% fatty acids, 6% cholesterol, 5% monoacyl glycerol, and 4% cholesterol ester.19854038247
[epidemiologic data on distomiasis due to fasciola gigantica cobbold on the island of madagascar. infestation of cattle between 1975 and 1981].stools examinations of zebus were made in madagascar between 1975 and 1981 for determining the importance of fascioliasis gigantica in the six provinces of the island during this time. high percentages of f. gigantica eggs were found in the stools of the different infected zebus-herds of five provinces. no fasciolid infection was noted in 1979 in the cattle of the province of diego-suarez. differences were noted between the infection rate obtained by fecal examination and the rate indicated in t ...19854075467
protection against fasciola gigantica infection in rats administered metacercarial antigens.immature worms were recovered from the liver parenchyma of rats which received extracts of metacercariae or adult worms of fasciola gigantica, after challenge infection. when resistance was assessed by counting the number of immature worms, exposure of the animals to extracts of these two stages of the flukes conferred a significant degree of protection against challenge with f gigantica metacercariae. rats given the extract of metacercariae were more resistant to infection than rats immunised w ...19854081346
the effect of niclofolan on desert sheep experimentally infected with immature fasciola gigantica.eight desert sheep were each infected orally with 500 metacercariae of fasciola gigantica and, after 4 weeks, four of the animals were given niclofolan orally at the recommended therapeutic dose rate of 7 mg/kg, the other four remaining as controls. one week later, the animals were slaughtered and the fasciocidal effect of the drug was evaluated on the basis of worm burden, haemogram, some plasma constituents, and gross and histopathological lesions of the liver, as indicators of efficacy. the t ...19854094030
serum enzyme activity and bilirubin concentration in sheep experimentally infected with fasciola gigantica.fasciola gigantica infection in sheep produced liver and lung lesions characterized by damage to blood vessels and parenchymal necrosis. in the lungs, the lesions were those of parasitic bronchopneumonia. the damage to the liver and lung tissues was accompanied by increased activity in serum of ast after 2 weeks, gd and sd after 4 to 5 weeks and ggt and 5'-nt from 8 weeks onwards. bilirubin concentration was not affected.19852866203
infectivity and pathogenicity of fasciola gigantica in west african dwarf sheep and goats.the infectivity and pathogenicity of fasciola gigantica for sheep and goats were investigated in animals infected with single doses of between 200 and 2,000 metacercariae. acute fascioliasis occurred in sheep infected with 1,000 to 2,000 cysts in which 10 to 17% of the cysts became established. the infected animals died 80 to 90 days after infection. in goats given the same infective doses death from acute fascioliasis occurred earlier (70 to 83 days after infection) and 12 to 38% of cysts becam ...19846485107
biology, life-tables, and intrinsic rates of increase of bulinus globosus morelet and lymnea natalensis kraus (gastropoda: pulmonata)--the snail intermediate hosts of schistosoma haematobium and fasciola gigantica, respectively, in southeastern nigeria.two tables and a figure are used to present the epidemiological and epizoological particulars of bulinus globosus and lymnaea natalensis, the intermediate hosts of schistosoma haematobium and fasciola gigantica in southeastern nigeria. possible ways of reducing the populations of these intermediate hosts are seen in the combined application of chemical agents and environmental shaping.19846529436
the effect of sex on seasonal variation in single and double infection of cattle in sierra leone by dicrocoelium hospes and fasciola gigantica.the incidence of dicrocoeliasis (61.8%) is higher than that of fascioliasis (25.5%) and their peaks do not occur at the same time of the year. the peak for the former occurs in the dry season while that of the latter occurs in the rainy season. the incidence of dicrocoeliasis and fascioliasis in female cattle was found to be higher than that of male cattle. double infection was higher in female (14.7%) than in male hosts (9.6%).19846541838
efficacy of ivermectin against internal parasites of sheep.ivermectin in an oral formulation was evaluated by the non-parametric method against a wide range of endoparasites of sheep in 15 trials involving a total of 297 sheep. ivermectin at 200 micrograms/kg was more than 80% effective in more than 80% of the treated animals (i.e. class a) against induced infestations of 3rd and 4th stage larvae and adults of chabertia ovina, dictyocaulus filaria, haemonchus contortus (including a benzimidazole-resistant strain), nematodirus spathiger, oesophagostomum ...19846549472
clinical diagnosis of schistosomiasis in sudanese cattle.this study was done in the white nile province to characterise the history and signs of naturally occurring schistosoma bovis infection in cattle (gorag). necropsy and laboratory examinations were performed on 10 animals six to 30 months of age which were in poor condition. they were selected because of a history suggestive of schistosomiasis. all the animals showed some degree of s. bovis infection; eight had a moderate or heavy degree of infection. also all had liver damage due to either past ...19836623642
effect of bithionol on b-glucuronidase in serum and liver of rabbits infected with fasciola gigantica. 19836631102
[epidemiology of hepatic fascioliasis (fasciola gigantica) at the dairy farm of the lubumbashi (zaire) ranch. results of a deparasitization trial. recommendations]. 19836672883
igg response in natural and experimental infections of cattle with fasciola gigantica.a specific igg response was detectable in serum and bile of cattle infected with fasciola gigantica. although there was an increase in both specific and total igg concentration in the sera of fluke-infected cattle, there was no significant correlation between igg1 concentration and the worm burden (r = 0.17; p greater than 0.05) in chronic fascioliasis. however, there was a highly significant correlation between the levels of igg1 in the serum and bile of infected cattle (r = 0.87; p less than 0 ...19836686385
chemosterilization and its reversal in the snail lymnaea acuminata.treatment of the snail lymnaea acuminata, the vector for the liver flukes fasciola hepatica and fasciola gigantica, with two alkylating agents (cyclophosphamide and busulfan) and one thiocarbamide (thiourea) caused reduction in the number of eggs and production of non-viable embryos. all the three drugs caused reduction in the levels of dna, rna and protein and the activity of alkaline phosphatase in the gonadal tissues of this snail. there was enhancement in free amino acid levels and activity ...19836189361
Displaying items 501 - 600 of 754