PMID(sorted descending)
[main features of helminth parasitism in cattle in ituri (haut-zaire). iii. geographic distribution and prevalence of the main helminths].a necropsic survey, carried out in eleven slaughterhouses in ituri (haut-zaïre), has permitted to define the geographic distribution and prevalences of the main cattle helminths. trematodes, except the paramphistomes, had a very heterogeneous distribution. prevalence of fasciola gigantica ranged from 9 to 72% according to the sites, but these variations were not linked to topographic or climatologic parameters. the infection with schistosoma bovis was much variable as well (12.5 to 72%) and seem ...19911775693
a purified fasciola gigantica worm antigen for the serodiagnosis of human fascioliasis.partially purified fasciola gigantica worm antigens were evaluated in an enzyme-linked immunosorbent assay (elisa) for the specific serological diagnosis of fascioliasis. the diagnostic potential of 10 antigen fractions derived from gel-filtration peaks (ii & iii) were evaluated individually or in combination against a panel of admission sera obtained from 50 patients subsequently diagnosed as having a variety of systemic parasitic diseases, and from 10 healthy volunteers with no recent history ...19911755065
control of fasciola gigantica with triclabendazole in indonesian cattle.the efficacy of triclabendazole (tcbz) against natural infections with fasciola gigantica was evaluated in a field study using 102 indonesian cattle. the animals were divided into two groups; one received tcbz at 12 mg/kg orally every eight weeks for one year while the other remained untreated. relative to controls the fasciola faecal egg count in treated animals was significantly reduced (p less than 0.01) to almost zero at all observations. there were no differences between the groups in blood ...19911686688
the effects of fascioliasis on the activities of some drug-metabolizing enzymes in desert sheep liver.desert sheep experimentally or naturally infected with fasciola gigantica were used to study the influence of infection on the activities of some drug-metabolizing enzymes found in the liver. the enzymes investigated were aminopyrine n-demethylase, aniline 4-hydroxylase and udp-glucuronyltransferase. the experimental infection was confirmed histologically by detection of fasciola eggs in faeces and by measuring the activities of sorbitol dehydrogenase (sd), glutamate dehydrogenase (gd) and aspar ...19921617399
cloning and characterization of ribosomal rna genes from opisthorchis viverrini.the ribosomal dna (rdna) unit of the liver fluke opisthorchis viverrini has been cloned and characterized. the results demonstrated that the total length of this unit is approximately 13 kb, containing 4.2 kb of large subunit (lsu) rdna, 2.0 kb of small subunit (ssu) rdna, 1.0 kb of transcribed spacer dna and 5.8 kb of non-transcribed + external transcribed spacer dna. examination of the non-transcribed spacer region between different rdna units showed variation in the restriction sites rather t ...19921594296
glycogen, protein and lipid content of adult fasciola gigantica (trematoda).the concentration of carbohydrates, proteins, and lipids in the whole worm, gut, and body musculature of fasciola gigantica was investigated from worms collected from cattle slaughtered in the jos abattoir. protein accounted for the highest concentration of the constituents constituting 62.59 +/- 0.86% of the dry weight of the fluke. this was followed by lipid with 28.15 +/- 0.86% of the dry weight while carbohydrate (glycogen) was 6.29 +/- 0.11% of the dry weight. thin-layer chromatography reve ...19921590595
seasonal prevalence of some zoonotic trematode infections in cattle and pigs in the north-east montane zone in india.seasonal variations in the prevalence of fasciola gigantica and eurytrema pancreaticum in cattle, and opisthorchis noverca, artyfechinostomum malayanum, fasciolopsis buski and gastrodiscoides hominis in pigs, were studied post-necropsy over a 1 year period in a humid, subtropical north-east hilly region in india. the overall prevalence rate was 53.02% in cattle (n = 960) and 12.92% in pigs (n = 960). fasciola gigantica and e. pancreaticum occurred throughout the year with peaks during cold winte ...19921561763
redial generations of fasciola gigantica in the pulmonate snail lymnaea truncatula.lymnaea truncatula, 4 mm in height, were subjected to infection by a single miracidium of fasciola gigantica, then raised at 23 degrees c until day 60 of the experiment. histological study of these snails demonstrated a mean redial burden of 34 parasites at day 60, of which one third were degenerating forms. the mean number of living independent rediae did not exceed 5 for the first and second generations. conversely, in subsequent generations there were as many as 18 rediae per snail at day 60. ...19921452990
the purification and characterization of a cysteine protease of fasciola gigantica adult worms.a 26-28 kda protease was isolated from fasciola gigantica adult worms by a two-stage purification process of column chromatography in a sephacryl s-200 column and affinity chromatography in an l-phenylalanine-agarose column. this protease is a cysteine (thiol) proteinase with an optimum ph of 4.5 and is not inhibited by anti f. gigantica immunoglobulin g. the enzyme was inhibited by protease inhibitors known to inhibit cysteine proteases but not by metallo-, aspartate or serine protease inhibito ...19921413454
larval stages of medically important flukes (trematoda) from vientiane province, laos. part ii. cercariae.the cercariae of five flukes, which are potential human parasites, opisthorchis viverrini (family opisthorchiidae), haplorchis taichui (heterophyidae), schistosoma spindale (schistosomatidae), fasciolopsis buski, and fasciola gigantica (fasciolidae) were found in freshwater snails from laos. the cercariae of the above species, recorded in laos for the first time, are illustrated and their morphology is briefly described. the snail helicorbis umbilicalis represents a new intermediate host of f. b ...19921290378
some clinico-pathological aspects of experimental fasciola gigantica infection in calves. 19761262690
fasciola gigantica cobbold 1856 in the snail.intramolluscan stages of fasciola gigantica were followed experimentally in laboratory-reared lymnaea natalensis, from the time of miracidial entry until the 85th day. contrary to previous accounts, the miracidium was found to metamorphose directly into a first-generation redia during the first 4 days. about the 16th day, the redia migrated to the snail liver. second-generation rediae gave rise to 3rd-generation rediae and/or cercariae by the 45th to 50th day. the release of cercariae from 3rd-g ...19761255351
the role of carbohydrates in the biology of the liver fluke, fasciola gigantica: glycogen content, its utilization and resynthesis by starved flukes. 19751225417
in vitro studies of the action of carbon tetrachloride on glucose consumption of fasciola gigantica. 19751225412
[attempted induction of acquired resistance to fasciola gigantica infestation]. 19751208923
the structure and cytochemistry of the neurosecretory cells of fasciola gigantica cobbold and fasciola hepatica l.histochemical studies of the nervous system of fasciola gigantica and fasciola hepatica were undertaken. neurosecretory cells were detected by gomori's aldehydefuchsin, bargmann's chrome hematoxylin-phloxin, mallory's triple stain, periodic acid-schiff, heidenhain's azan and alcian blue after potassium permanganate oxidation. two types of neurosecretory cells were recognized and designated as "a" and "b". type "a" cells occurred in small numbers in the brain and subesophageal mass and type "b" c ...19751189578
clinico-pathological studies on naturally-occurring bovine fascioliasis in the investigation was made of the pathological, haematological and biochemical aspects of naturally-occurring bovine fascioliasis in the sudan. 228 animals infected with fasciola gigantica and 25 non-infected controls were used in the study. the infected cattle revealed emaciation, typical liver pathology, and, occasionally, lesions in the lung and the pancreas. analysis of their sera also showed reduced albumin values, increased globulin concentrations and decrease albumin/globulin ratio, in add ...19751184942
[the efficacy of dirian against fasciola gigantica and paramphistomes in naturally infected cattle in madagascar]. 19751164394
the comparative efficacy of diamphenethide and rafoxanide against fasciola gigantica in sheep.efficacy of diamphenethide ("coriban", burroughs wellcome) and rafoxanide ("ranide", merck sharpe & dohme, ltd.) was evaluated in lambs against 8-week old experimentally induced fasciola gigantica infection. results were assessed by the reduction in worm counts at post-mortem examination 8 weeks after treatment. diamphenethide at a dosage rate of 100 mg/kg body weight was found 99.4 per cent effective. rafoxanide at a dosage rate of 10 mg/kg body weight showed 98.78 per cent efficacy. six weeks ...19751162735
methods for in vitro study of the invasive processes of fasciola vitro techniques have been developed to study the invasive processes of fasciola gigantica. the conditions necessary for excystment were identical with those rquired by f hepatica. the ability of larvae to penetrate mouse gut in vitro was influenced by the composition of the fluid within the gut, and by the region of gut wall to which the larvae were exposed. larvae remained viable on spleen cell monolayers for at least 60 days.19751153904
the corticosteroid content of cattle skin vitro techniques have been developed to study the invasive processes of fasciola gigantica. the conditions necessary for excystment were identical with those required by f hepatica. the ability of larvae to penetrate mouse gut in vitro was influenced by the composition of the fluid within the gut, and by the region of gut wall to which the larvae were exposed. larvae remained viable on spleen cell monolayers for at least 60 days.19751153903
v. effect of enzymes and temperature on the serological activity of the antigen of fasciola gigantica.the partially purified extract of fasciola gigantica was incubated with enzyme pepsin and samples were taken at different time intervals from 0-24 hours, and tested intradermally for diagnosing bilharziasis. the enzymatic degradation of the protein molecule results in the progressive decrease and eventually the complete disappearance of serological activity. this showed that the protein portion of the antigen was deemed necessary for its activity in intradermal tests for diagnosing schistosomias ...19751095775
trematodes of domestic ruminants of afghanistan and their role in trematode species were found in the bile ducts and rumen of domestic ruminants: fasciola gigantica, f. hepatica, gigantocotyle explanatum, g. siemense, paramphistomum epiclitum, fischoederius cobboldi and gastrothylax cruminifer. the parasites caused marked pathological changes in the liver and rumen.19761010490
in vitro maintenance of juvenile fasciola gigantica and their use to establish infections in mice.studies on the growth of juvenile fasciola gigantica in vitro on tissue culture feeder layers and their subsequent infectivity for mice have indicated that although no significant growth occurs in vitro the juveniles are not damaged physiologically for at least six weeks. thus the in vitro system may be used with confidence in studies of the physiology of newly excysted flukes. significantly more flukes became established in mice from an intraperitoneal injection of juveniles than from an oral i ...1976968196
[rafoxanide in the treatment of fasciola gigantica fascioliasis in the zebu of chad]. 1976959621
an in vitro study of the relative importance of bile and carbon dioxide in the activation of fasciola gigantica metacercariae.the relative importance of bile and atmospheric stimuli in activating metacercariae of fasciola gigantica prior to excystment has been studied by omitting each in turn from the in vitro excystment medium, and by separating each from the period of incubation at 37 degrees c. the results indicate that bile is less important than carbon dioxide in triggering excystment, and may serve only to increase the permeability of the cyst wall to carbon dioxide. it is speculated that activation involves init ...1976935671
occurrence of a sporocyst generation of fasciola gigantica in the mollusc lymnaea rubiginosa.thirty laboratory-reared, juvenile lymnae rubiginosa were exposed to 20 miracidia each of fasciola gigantica. eight days postinfection snails were dissected, and clearly recognizable sporocysts were recovered from the mantle collar and renal vein. behavior of sporocysts and mother rediae differed markedly. these observations contradict those of direct metamorphosis of miracidium into redia as reported by ogambo-ongoma and goodman (1976).1977859085
the pattern of parasitic infection in human gut at the specialist hospital, benin city, a survey of 6213 persons conducted between january 1973 to december 1974, at the specialist hospital, benin city, the most common helminths were necator americanus (16.8%), ascaris lumbricoides (19l5%) and trichuris trichiur (5-9%). dicrocoelium hospes (0-06%) was also recorded and this may become an important liver parasite of man in nigeria. its snail vectors are believed to be species of limicolaria and achatina which are widely dispersed in nigeria. entamoeba coli and e. histolytica showe ...1977849017
laboratory investigations on natural infection in zebu cattle with fasciola gigantica and schistosoma bovis. 1977835347
initial fractionation of adult schistosoma bovis antigen for diagnosis of infection in cattle.initial work on the fractionation of schistosoma bovis adult worms is described. both male and female worms were homogenized and sonicated before being passed through sephadex g200. five peaks were obtained with male fractionation (designed m1 to m5) and four with female (designated f1 and f4). using the complement fixation test s. bovis antigen activity was detected primarily in peaks m1 and f1. with the indirect haemagglutination and indirect haemagglutination inhibition tests s. bovis antigen ...1975805168
hepatic changes in natural fasciola gigantica infestations of the fulani zebu. 1978749966
[effectiveness of injectable rafoxanide against the adult fasciola gigantica in the madagascar zebu]. 1978734202
[capacity of resistance to dryness of the snail (limnaea natalensis), an intermediate host of fasciola gigantica in senegal]. 1978715292
viability of fasciola gigantica metacercariae. 1978671936
studies on the intestinal parasites in african patients in owamboland, south west africa.the paper gives the results of a survey of intestinal parasites among 501 in-patients drawn from four mission hospitals in owambo and kavango in northern south west africa. most of the patients (90%) were examined twice by the formol-ether concentration method, while the remaining 10% had one stool specimen examined. 10 species of helminths and 3 species of potentially-pathogenic protozoa were recorded--necator americanus, strongyloides stercoralis, strongyloides fuelleborni, hymenolepis nana, t ...1978635971
enzyme histochemistry of fasciola gigantica. 1978631652
a survey on liver infections with fasciola gigantica among slaughtered animals in iraq. 1977617942
schistosoma mattheei in the ox: the serological response.thirty friesian steers were infected with schistosoma mattheei and the antibody response was followed for up to 76 weeks by the complement fixation (cf), indirect haemagglutination (ih) and indirect immunofluorescent (if) tests. cf and if antibodies rose to a peak at about 25 weeks and then fell, while ih antibodies rose more slowly and remained high. peak ih and if titres were proportional to the level of infection. peak cf titres were reduced in animals on a low plane of nutrition. there was a ...1977605296
parasites of domestic and wild animals in south africa. x. helminths in impala.two to four impala in the nylsvley nature reserve were culled each month from february 1975-february 1976. two trematode species, 1 cestode species and 13 species of nematodes were recovered from these antelope. of these, fasciola gigantica, gongylonema pulchrum, haemonchus placei and trichostrongylus falculatus are new records for impala. in general, h. placei, longistrongylus sabie and impalaia tuberculata exhibited a similar pattern of seasonal occurrence. adult worms were present during nove ...1978572950
nitroxynil. anthelmintic activity in cattle following subcutaneous injection.nitroxynil injected subcutaneously at 10 mg/kg live mass achieved class a efficacy when evaluated by the non parametric method against adult fasciola gigantica. haemonchus placei, bonustomum phlebotomum and oesophagostomum radiatum in cattle. the compound was not effective against adult cooperia spp. at the same dosage.1978569707
a preliminary observation on the pathogenicity of fasciola gigantica in pregnant west african dwarf ewes. 1979554281
the epidemiology of fascioliasis in malawi: i. the epidemiology in the intermediate host.fasciola gigantica cobbold was studied in the intermediate host snail. on snail survey lymnaea natalensis krauss was the species found responsible for the transmission of fasciola gigantica in malawi. the ecology of this snail was studied at 5 different habitats spread throughout malawi. the snail population was recorded high from april to october. the epidemiological cycle of fasciola gigantica in the snail was determined and it was shown that snail infection was high in april and may and thus ...1979552677
effect of anthelmintic treatment on the performance of awassi sheep in iraq.studies are reported on the effect of anthelmintic treatment on the body weight gain and wool growth of ewes with subclinical helminth infections. the parasites involved were fasciola gigantica and ostertagia spp., together with some haemonchus contortus, trichostrongylus spp., and dictyocaulus filaria. in 1 group, 5 sheep, which were treated twice with tetramisole at an interval of 4 weeks to remove gastro-intestinal nematodes and lungworms, showed an increase of 41% in weight gain and 3.2% in ...1979552676
the anthelmintic efficacy of albendazole against gastrointestinal roundworms, tapeworms, lungworms and liverflukes in sheep.anthelmintic trials were carried out to evaluate the efficacy of albendazole against helmi of 2,5 to 3,8 mg/kg administered orally, resulted in a 98,8 to 100% reduction of adult parasites of the genera haemonchus, ostertagia, trichostrongylus, nematodirus, gaigeria, oesophagostomum, chabertia, marshallagia and cooperia. against the immature stages of these genera, except for marshallagia and cooperia, which were not tested, a dose level of 2,5 to 3,8 mg/kg was 83,9-100% effective. albendazole at ...1979551183
an aberrant location of fasciola gigantica in spleen and lung of indian buffaloes (bubalus bubalis) and pathological study. 1979536042
assessment of the attenuation produced by irradiation of fasciola gigantica metacercariae in hamsters.severe pathological changes were seen in hamsters given metacercariae of fasciola gigantica irradiated at doses below 4 krads. no adult flukes were recovered from hamsters given metacercariae irradiated at doses above 4 krads and little hepatic damage was caused. it was not ascertained whether resistance resulted following these latter infections.1979523813
ovine facioliasis (fasciola gigantica) on the ahmadu bello university farm.outbreaks of fascioliasis (fasciola gigantica) in sheep were observed on the ahmadu bello university farm during the end of the dry season in 1972-74 and in 1978. during 1972 especially the pregnant ewes were affected. pre- and post-mortem exmaination of the affected sheep showed typical signs of acute fascioliasis; the number of flukes in the liver ranged between 77 and 239. the infections were acquired when the animals grazed near the man-made lake on the farm. a limited snail survey revealed ...1979505587
the serodiagnosis of chronic fascioliasis (fasciola gigantica) using a fluorescent-antibody technique with single and multiple whole-fluke antigens.sera from cattle with single or mixed infections of fasciola gigantica. dicrocoelium hospes, schistosoma bovis and paramphistomes were examined for fluorescent antibodies. cross-reacting antibodies of the heterologous flukes were easily detected by using cryostat sections with whole-fluke-antigen of all three flukes. the presence of f. gigantica antibodies in a cattle herd was tested at two monthly intervals in order to establish the occurrence of chronic fascioliasis. the lack of significant di ...1979384628
antibody response of calves to a single infection of fasciola gigantica determined by an indirect fluorescent antibody technique.the antibody response of six calves infected with 500 metacercariae of fasciola gigantica each was monitored throughout 30 weeks of infection using an indirect fluorescent antibody technique (ifa). in vitro excysted f gigantica were employed as the test antigen. all animals showed high antibody titres from two to six weeks post-infection. thereafter the antibody titres diminished gradually. although all the experimentally infected animals harboured fluke burdens at autopsy, most gave negative if ...1977327523
trials with rafoxanide. 8. efficacy of an injectable solution against trematodes and nematodes in cattle.four experiments are described in which the efficacy of an experimental 5% injectable solution of rafoxanide was evaluated against various adult and immature helminths in cattle. subcutaneous injection at a dosage of 3 mg/kg live mass resulted in the following reductions in mean worm burdens: adult fasciola hepatica, 82,6%; adult fasciola gigantica, 99,8% immature paramphistomum microbothrium, 10,1% adult haemonchus placei, 99,6%, third stage h. placei, 73,7%; adult bunostomum phlebotomum, 99,8% ...1977144191
changes in serum protein values of sheep infected with fasciola gigantica.serum protein values were determined in 6 lambs given 1 dose of 200 metacercariae of fasciola gigantica. over the 22 weeks of the experiment, total serum protein values in these lambs were only moderately increased over those of control lambs (n = 6). the increase was mainly associated with the change in alpha2-globulin and gamma-globulin fractions. the highest value of gamma-globulin (39.03%) of total serum protein was observed at 18 weeks after infection. mean albumin concentration in the expo ...197656910
fasciola hepatica and fasciola gigantica: total cholinesterase, characteristics, and effects of specific inhibitors. 197716762
the role of carbohydrates in the biology of the liver fluke, fasciola gigantica: effects of temperature and ph upon glucose uptake. 19755167
non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes.alkaline phosphatases from different trematodes occupying the same habitat have identical ph otima but different levels of enzyme activities. isoparorchis hypselobagri, from the fish wallago attu, shows four to six times more enzyme activity than fasciolopsis buski, gastrodiscoides hominis and echinostoma malayanum, from the pig sus scrofa, and fasciola gigantica, gigantocotyle explanatum, cotylophoron cotylophorum and gastrothylax crumenifer, from the buffalo bubalus bubalis. at least two peaks ...19751442
Displaying items 701 - 754 of 754