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[the effect of hetol on immature and mature stages of fasciola gigantica in artificially infected sheep]. 19675628510
[distomatosis in madagascar. the fasciola gigantica cycle]. 19675625568
[fasciola gigantica (tropical fluke) hepatic distomatosis. importance of the mental disorders]. 19675599911
[differences between the testicular morphology of fasciola hepatica and fasciola gigantica]. 19705535130
[treatment in tropical africa of hhepatobiliary fascioliasis caused by fasciola gigantica in zebu cattle. value of bilevon r bayer]. 19705534364
the pathology of experimental infection of cattle with fasciola gigantica. 19705526751
the experimental demonstration of lymnaea lagotis euphratica as an intermediate host of fasciola gigantica in iraq. 19705500109
[distomiasis in madagascar: use of molluscacides in the campaign against lymnaea natalensis hovarum (intermediate host of fasciola gigantica)]. 19695407991
[therapy and early diagnosis of fasciola gigantica infection in sheep]. 19695388487
studies on the activity of nitroxynil against fasciola gigantica in naturally infected buffaloes, cattle and sheep. 19695387806
a type of cercaria easily mistaken for fasciola gigantica in uganda. 19695386286
experimental infection of calves with fasciola gigantica. 19695362596
[anthelmintic activity of a halogen derivative of salicylanilide against fasciola gigantica]. 19715169494
oxyclozanide--activity against fasciola gigantica in naturally infected buffalo, cattle, sheep and goats. 19715164589
trials with rafoxanide. 2. efficacy against fasciola gigantica in cattle. 19715161408
trials with rafoxanide. 1. fasciola gigantica in cattle in angola. 19715161407
[method of evaluation of the effects of various anthelmintic drugs in relation to the immature forms of fasciola gigantica]. 19715153730
[role of nutritional factor in bovine and ovine distomatosis caused by fasciola gigantica]. 19715153729
a comparison of the activity of some fasciolicides against immature fasciola gigantica in experimentally infected calves. 19715153728
the life cycle of fasciola gigantica cobbold, 1885 i. the sporocyst myth? 19715153727
[apropos of the action of bitin-s and of bromophenophos on immature fasciola gigantica]. 19715106008
the occurrence of fasciola gigantica in the liver of an indian rhinoceros (r. unicornis). 19715103964
[study on hypersensitivity, precipitating antibodies and complement fixing antibodies during bovine infestation by fasciola gigantica]. 19715002886
[apropos of a case of hepatic distomatosis due to fasciola gigantica contracted in cameroon]. 19684973929
[case of hepatic distomatosis due to fasciola gigantica. diagnosis in the early stage of bilharziosis due to schistosoma mansoni]. 19684922836
lymnaea natalensis laboratory culture and production of fasciola gigantica metacercariae. 19684905987
a new technique for the recovery of fasciola gigantica eggs from cattle faeces. 19674887044
[fasciola gigantica infestation in small laboratory animals]. 19744838609
hepatic calcification due to fasciola gigantica. 19744808568
[a case of human fasciola gigantica douve. simultaneous hepatic and subcutaneous infestation]. 19734801860
[phacochoerus aethiopicus (pallas), new host of fasciola gigantica cobbold, 1855]. 19734801778
liver fluke fasciola gigantica in african buffalo and antelopes in uganda, east africa. 19724673717
trials with rafoxanide. 5. efficacy studies against fasciola hepatica, fasciola gigantica, paramphistomum microbothrium and various nematodes in sheep. 19724670768
a preliminary note on the susceptibility, prepatency and recovery of fasciola gigantica in small laboratory animals. 19724641407
note on the sporocyst in fasciola gigantica. 19744471455
[distomatosis of the domestic rabbit caused by fasciola gigantica]. 19744469355
the role of carbohydrates in the biology of the liver fluke, fasciola gigantica: utilization of exogenous glucose. 19744465014
the role of carbohydrates in the biology of the liver fluke, fasciola gigantica: on the active absorption of glucose. 19744465012
vertical distribution of fasciola gigantica metacercariae on stems of rice plant grown in a water pot. 19744444734
the pathogenic effect of experimental infections with fasciola gigantica in cattle. 19744434159
human infection with the liver fluke fasciola gigantica. 19744422226
biochemical and histochemical studies on the non-specific phosphomonoesterases of fasciola gigantica cobbold 1855. 19724336211
preliminary observations on antigen activity of fasciola gigantica by immunodiffusion test in sheep. 19744212259
notes on glycogen deposits in limnaea auricularia var. rufescens and the parasitic larval stages of fasciola gigantica and echinostoma revolutum. a histochemical study. 19684190113
the effect of niclofolan on desert sheep experimentally infected with immature fasciola gigantica.eight desert sheep were each infected orally with 500 metacercariae of fasciola gigantica and, after 4 weeks, four of the animals were given niclofolan orally at the recommended therapeutic dose rate of 7 mg/kg, the other four remaining as controls. one week later, the animals were slaughtered and the fasciocidal effect of the drug was evaluated on the basis of worm burden, haemogram, some plasma constituents, and gross and histopathological lesions of the liver, as indicators of efficacy. the t ...19854094030
protection against fasciola gigantica infection in rats administered metacercarial antigens.immature worms were recovered from the liver parenchyma of rats which received extracts of metacercariae or adult worms of fasciola gigantica, after challenge infection. when resistance was assessed by counting the number of immature worms, exposure of the animals to extracts of these two stages of the flukes conferred a significant degree of protection against challenge with f gigantica metacercariae. rats given the extract of metacercariae were more resistant to infection than rats immunised w ...19854081346
[epidemiologic data on distomiasis due to fasciola gigantica cobbold on the island of madagascar. infestation of cattle between 1975 and 1981].stools examinations of zebus were made in madagascar between 1975 and 1981 for determining the importance of fascioliasis gigantica in the six provinces of the island during this time. high percentages of f. gigantica eggs were found in the stools of the different infected zebus-herds of five provinces. no fasciolid infection was noted in 1979 in the cattle of the province of diego-suarez. differences were noted between the infection rate obtained by fecal examination and the rate indicated in t ...19854075467
studies on the energy reserve of fasciola gigantica from nigerian zebu cattle.as energy reserves in the body tissue of mature liver flukes var. fasciola gigantica from the bile ducts of nigerian zebu cattle the following percentages were found: protein 60.29, lipid 11.92, carbohydrate 7.0. the analysis of the lipid fractions showed the following composition: 47% phospholipids (63% phosphatidyl choline, 28% phosphatidyl serine, 9% lysophosphatidyl choline) and 22% triglycerides, 16% fatty acids, 6% cholesterol, 5% monoacyl glycerol, and 4% cholesterol ester.19854038247
experimental cholecystitis in goats caused by mature fasciola gigantica.mature fasciola gigantica obtained from naturally infected cattle were surgically transferred into the gallbladders of six fluke-free goats. two of the goats died at two and 19 weeks and the remaining four were slaughtered at 12, 48, 48 and 80 weeks after the transfer. nine of 20 transferred f gigantica were recovered from the gallbladder and common hepatic duct of the experimental goats. the walls of gallbladders and bile ducts were thickened by epithelial proliferation, fibrosis and mononuclea ...19854012032
detection of fasciola gigantica eggs in faeces: a comparison of methods. 19873824857
immunization of zebu calves against fasciola gigantica, using irradiated metacercariae.the pathogenesis of unirradiated, 3 krad-irradiated and 20 krad-irradiated metacercarial infections was compared in zebu calves studied over a 10-week period. calves exposed to 1000 unirradiated metacercariae (mc) became hypoalbuminaemic, and showed elevated serum concentrations of liver enzymes, whereas neither of the other groups was significantly affected. at slaughter, a mean of 332 flukes was recovered from the 0 krad group, while only 23% and 12% of this number were recovered from the 3 kr ...19863734396
some biochemical indices in naturally occurring fascioliasis in goats.some biochemical indices were assayed in goats naturally harbouring fasciola gigantica infection and compared with uninfected control goats. infected goats had significantly lower levels of serum glucose (47.6 +/- 1.8 mg dl-1) and albumin (3.1 +/- 0.1 g dl-1) and reduced albumin:globulin ratio (1.1 +/- 0.1). total lipid (526.8 +/- 2.4 mg dl-1), serum glutamate dehydrogenase (15.3 +/- 0.9 iu litre-1) and serum alkaline phosphatase (31.6 +/- 1.9 kind's and king's unit dl-1) were high in infected g ...19863704349
studies on heterologous resistance between schistosoma bovis and fasciola gigantica in sudanese cattle.using the local strains of schistosoma bovis and fasciola gigantica, it was shown that sudanese zebu calves with mature primary infections of f. gigantica were highly resistant to challenge with s. bovis cercariae, and vice versa. liver enzyme tests showed that, in both cases, the primary infections had caused some liver damage. primary infection with irradiated s. bovis cercariae, which did not cause significant liver damage, did not protect significantly against challenge with f. gigantica.19863701021
studies on naturally-occurring ovine fascioliasis in the sudan.haematological, biochemical and pathological changes were investigated in 214 sheep naturally infected with fasciola gigantica in an endemic area in the sudan together with 82 uninfected controls. infected animals showed a clear decrease in erythrocyte counts, haemoglobin concentration and packed cell volume, a normochromic, normocytic anaemia, leucocytosis and eosinophilia. serum concentrations of the enzymes glutamate dehydrogenase, sorbitol dehydrogenase and glutamate oxaloacetic acid transam ...19863701020
differentiation of the rediae of fasciola hepatica and fasciola gigantica. 19863632105
the protective effect of irradiated metacercariae of fasciola gigantica against homologous challenge in sheep.five lambs were each sensitized with 400 metacercariae of fasciola gigantica irradiated at 3 kr gamma rays. eight weeks later, they were each challenged together with 5 controls with 500 non-irradiated metacercariae. eight weeks later, all of the animals were necropsied and the worm burdens determined. the lambs which received the sensitizing infections had 80% less worms than the controls. the sensitized lambs also showed less hepatic damage compared with the controls as indicated by lower leve ...19873611708
fasciola gigantica in gallbladder. 19873583338
serodiagnosis of fascioliasis in buffaloes by immunoprecipitation using adult fasciola gigantica antigens.partial purification of a saline extract of adult fasciola gigantica by chromatography on sephadex g200 gave four peaks, of which only the first two contained material which gave a precipitation reaction with sera from infected buffaloes. none of these gave false reactions with sera from uninfected animals but even the first and most sensitive fraction only reacted with 57.5 per cent of sera from animals known to be infected with moderate numbers of f gigantica.19873547527
hepatic fascioliasis in the abattoirs of basrah.hepatic fascioliasis due to fasciola gigantica among slaughtered animals was studied at the basrah abattoirs from october 1984 to september 1985. the infection rates were 4.8, 3.3, 0.72 and 0.13% for buffaloes, cattle, sheep and goats respectively. the approximate annual loss due to condemnation of infected livers is estimated as 29,855 iraqi dinars.19873446027
studies on the energy reserve of fasciola gigantica (cobb.) from nigerian zebu cattle. ii. the fatty acid composition.the fatty acids were studied of the total lipid extracts of mature liver flukes fasciola gigantica from the bile ducts of nigerian zebu cattle. the high content of saturated fatty acids, which amounted to more than 54% of the total fatty acids, conferred a high level of saturation on these parasites. this was brought about by a general increase in the values of palmitic acid. the results are discussed in connection with the parasite-host relationship.19873426530
protective effect of irradiated metacercariae of fasciola gigantica and irradiated cercariae of schistosoma bovis against fascioliasis in goats.sensitisation of goats for eight weeks with metacercariae of fasciola gigantica gamma-irradiated at 3 kr resulted in significant resistance to an homologous challenge with normal metacercariae. however, serum sorbitol dehydrogenase assay suggested that, whereas little damage was produced by immunising infections, considerable hepatic damage was caused by flukes remaining from challenge infections. on the other hand sensitisation with cercariae of schistosoma bovis gamma-irradiated at 3 kr did no ...19873424453
liver fluke infections of cattle in mali. an abattoir survey on prevalence and geographic distribution.six hundred and ninety two cattle, slaughtered in various abattoirs in mali between august 1983 and november 1984 were examined for evidence of fasciola gigantica and dicrocoelium hospes infections. a great variation in geographic prevalence was observed. sufficient knowledge was acquired to demonstrate both the importance of fascioliasis in some areas and also the high prevalence of d. hospes in other areas. we suggest that the difference in prevalence from one geographic area to another is inf ...19883400112
[apropos of the origin of some diseases raging in madagascar].madagascar had been separated from african continent since 5 millions years and, if flora and fauna are endemic (40 species of lemuriens) all of parasites and humans populations are imported, from africa with respect to p. falciparum, p. malariae, s. mansoni and s. haematobium (but snails are endemic ), fasciola gigantica (imported with zebus) and perhaps p. vivax, and from asia with respect to w. bancrofti, n. americanus, c. cellulosae (imported with pigs) and perhaps p. vivax, chromoblastomyco ...19873326688
fasciola gigantica: pathological and helminthological observations in experimental infection of yankassa lambs. 19883253861
fasciola gigantica: the second case of human infection in thailand. 19883251969
environmental stress and parasitic infections: ii. effects of gramoxone and hexadrin (pesticides) on the survival characteristics of fasciola gigantica miracidia.miracidia of fasciola gigantica have a maximum longevity of 9 h. miracidial survival was found to be age dependent and the instantaneous mortality rate increased exponentially with miracidial age and pesticide treatment. mortality of the pesticide-treated miracidia differs significantly from that of the control. the lc50 value for hexadrin was calculated at 1.93 ppm and for gramoxone at 0.75 ppm. dosage-mortality lines when tested statistically show no significant difference between the two pest ...19883245635
on the viability of fasciola gigantica metacercariae ingested by lymnaea auricularia.the effect of ingestion by lymnaea auricularia on the viability and infectivity of fasciola gigantica metacercariae was studied. the cyst wall was unaffected by the snail's digestive processes. two rabbits, each infected with 50 ingested metacercariae, died at 83 and 87 days post-infection. eight and 10 immature flukes were recovered from the livers, indicating that the metacercariae had remained infective after passing through the intestine of the snail.19883235797
[role of nitroxynil in the integrated control of fasciola gigantica fasciliasis in cattle in madagascar]. 19883217565
environmental stress and parasitic infections: i. the effects of gramoxone and hexadrin on embryo and hatching of fasciola gigantica miracidia.the effect of two chemical compounds commonly used in agriculture, gramoxone (a herbicide) and hexadrin (an insecticide) on embryonic development and hatching of fasciola gigantica miracidia were experimentally assessed. these two pesticides were introduced in varying quantities into petri dishes containing unembryonated eggs of the trematode for a period of 30 days. lc50 (lethal concentration for 50% hatching) values were determined for them. for gramoxone it was 2 ppm and for hexadrin it was 2 ...19883202331
the effect of levamisole (l. tetramisole) treatment on the susceptibility to fasciola gigantica infection in goats. 19883201025
fasciola in horses in the republic of south africa: a single natural case of fasciola hepatica and the failure to infest ten horses either with f. hepatica or fasciola gigantica.the faeces of 11 horses were examined for fasciola spp. eggs. one of them was positive for fasciola hepatica, a finding which was confirmed post-mortem. the 10 negative horses were subsequently infested with either f. hepatica or fasciola gigantica, each animal receiving orally from 500-9,500 metacercariae. no clinical signs were observed and no fluke eggs were detected in the faeces, and neither immature nor adult fasciola worms were recovered from the horses slaughtered 16-26 weeks post-infest ...19883194116
serodiagnosis of fasciola gigantica infection in buffaloes.the agar gel precipitation test (agpt), counter immunoelectrophoresis (ciep) and indirect haemagglutination (iha) were evaluated for the diagnosis of fascioliasis due to fasciola gigantica in buffaloes. the sensitivity of these tests varied with the intensity of infection; and was greatest when the fluke burden in liver exceeded 100. ciep detected 76.06% of infected sera and was most sensitive, followed by iha which detected 68.37% of the infected sera. the agpt was found to be least sensitive, ...19873109111
diacetyl reductase activity in the extracts of fasciola gigantica. 19883059781
studies on the prevalence and laboratory transmission of fascioliasis in animals in the kashmir valley.in kashmir, 85.1% of cattle, 51.3% of sheep and 14.8% of goats were found infected with fasciola spp. the prevalence rate varied from 66.6 to 100.0%, 25.0 to 100% and nil to 66.0% in cattle, sheep and goats respectively in different months of the year. fasciola gigantica was the predominant species in all animal species but sheep harboured both f. gigantica and f. hepatica. the prevalence of f. hepatica infection in sheep happens to be the first report from india. lymnaea auricularia sensu stric ...19892920277
serum enzyme activity and bilirubin concentration in sheep experimentally infected with fasciola gigantica.fasciola gigantica infection in sheep produced liver and lung lesions characterized by damage to blood vessels and parenchymal necrosis. in the lungs, the lesions were those of parasitic bronchopneumonia. the damage to the liver and lung tissues was accompanied by increased activity in serum of ast after 2 weeks, gd and sd after 4 to 5 weeks and ggt and 5'-nt from 8 weeks onwards. bilirubin concentration was not affected.19852866203
comparative study of sheep and buffalo isolates of fasciola gigantica in the intermediate host lymnaea auricularia.a comparison was made of the biological behaviour of two isolates of fasciola gigantica, obtained from sheep and buffalo, in the aquatic snail lymnaea auricularia. the sheep isolate exhibited a lower rate of infection, slower larval development and the production of fewer cercariae. larger eggs, miracidia and metacercariae were obtained from the buffalo isolate while the rediae and cercariae were smaller. metacercariae of sheep origin were dark brown with a mortality after 80 days of storage at ...19892799087
sensitivity of crude and purified fasciola antigens in immunological diagnosis of human fascioliasis.a study has been done to find the effect of purification of crude antigens, extracted from adult fasciola gigantica and fasciola hepatica worms on the sensitivity of some serological techniques in diagnosis of fascioliasis. using c.i.e.p. test, the partially purified antigens of both fasciola species were less sensitive than their corresponding crude ones. using i.h.a., the difference between results of crude and partially purified antigens for the two species were statistically insignificant, b ...19892768848
response of goats to repeated infections with fasciola gigantica.one or two mature primary infections with fasciola gigantica which had been removed by anthelmintic treatment resulted in a significant reduction in the number of flukes recovered from challenge infection as compared with that from controls. characteristic lesions of fascioliasis were seen in the livers of the 3 groups, however, goats with two primary abbreviated infections prior to challenge showed more severe lesions than those of animals with one primary abbreviated infection or those of chal ...19892728319
acute cholecystitis and fasciola sp. infection in thailand: report of two cases.two cases of fascioliasis gigantica from northeast thailand presenting with cholecystitis and gall stones were reported. both cases complained of abdominal pain. on laparotomy the worms were found. in one case five worms were recovered during bile duct exploration and bile drainage; eggs were also revealed in the bile. in the other case of ectopic fascioliasis one young adult worm was found in a nodule which adhered to liver and diaphragm. the parasites were identified as fasciola gigantica.19892699088
specific detection of circulating surface/secreted glycoproteins of viable cysticerci in taenia saginata cysticercosis.a mouse monoclonal antibody (moab), reactive with a repetitive carbohydrate epitope on lentil-lectin adherent glycoproteins present on the surface and in the secretions of taenia saginata cysticerci, was used in the construction of a diagnostic elisa assay to detect these glycoproteins in the serum of t. saginata infected cattle. the moab was used as the trapping layer and subsequently bound glycoprotein was revealed using the same moab and biotinylated peroxidase-streptavidin complex as the dev ...19892674862
susceptibility of japanese lymnaea ollula to nigerian fasciola gigantica. 19892585940
restriction enzyme mapping of ribosomal dna can distinguish between fasciolid (liver fluke) species.recognition sites for nine different restriction endonucleases were mapped on rdna genes of fasciolid species. southern blots of digested dna from individual worms were probed sequentially with three different probes derived from rdna of schistosoma mansoni and known to span between them the entire rdna repeat unit in that species. eighteen recognition sites were mapped for fasciola hepatica, and seventeen for fasciola gigantica and fascioloides magna. each fasciolid species had no more than two ...19892552311
properties of phenol oxidase in fasciola gigantica.phenol oxidase of fasciola gigantica exists both as the soluble form as well as the membrane-bound form. the membrane-bound enzyme is considered to be a tyrosinase type because it is capable of oxidizing mono- and diphenol and is inefficient in oxidizing paraphenols. the soluble enzyme is a laccase type showing more affinity to various diphenols and paraphenols. membrane-bound enzyme exists as isoenzymes, showing 3 fractions, of which the slow-moving fraction is capable of oxidizing both 4-methy ...19892514408
[principal helminths of small ruminants in ituri (upper zaire)].from august 1986 to november 1987, about 180 slaughtered sheep and goats were examined at bunia (haut-zaïre) in order to establish the main helminth parasites. seventeen and fifteen species were identified in sheep and goats respectively. the occurrence of stilesia hepatica, oesophagostomum multifoliatum and muellerius capillaris as well as the absence of echinococcus granulosus constituted the original features of this check-list in the area. parasitism the most frequently encountered both in s ...19902369171
antigens and antibodies in the ascitic fluid of mice infected with fasciola gigantica.fluke antigens were detected in the ascitic fluid of mice infected with fasciola gigantica. the precipitating antibodies reactive against the antigens from metacercariae were detected as early as 2 weeks after the infection, and those against the adult and immature worms appeared 3 and 4 weeks later.19902343528
comparative evaluation of the enzyme-linked immunosorbent assay (elisa) in the diagnosis of natural fasciola gigantica infection in cattle.the enzyme-linked immunosorbent assay (elisa) was evaluated for the diagnosis of naturally acquired fasciola gigantica infection in cattle in comparison with conventional parasitological techniques. using unfractionated whole worm extract of f. gigantica as the antigen, it was observed that there was a good correlation between elisa positivity and positive diagnosis of fascioliasis by post mortem liver examination, bile egg sedimentation and faecal egg sedimentation. there was, however, a dispar ...19902321350
countercurrent immunoelectrophoresis in the diagnosis of taenia hydatigena cysticercosis in goats.the procedure of countercurrent immunoelectrophoresis in the diagnosis of taenia hydatigena cysticercosis in goats was carried out for antemortem diagnosis of t. hydatigena cysticercosis in experimentally and naturally infected goats. the antigens of cyst fluid, scolex and membrane of t. hydatigena metacestodes were purified and compared. the sensitivity of the test in experimentally and naturally infected goats was 57.1 and 52.5%, respectively, whereas its specificity using antisera raised agai ...19902267724
causes of mortality in impala (aepyceros melampus) on 20 game farms in zimbabwe.the most common cause of death in impala (aepyceros melampus) herds on 20 game farms in zimbabwe was stress or physical injury during capture. acute fascioliasis due to fasciola gigantica was identified as the main disease problem associated with smaller game farms and where impala had been recently introduced. losses associated with ticks and tick-borne diseases were rare. impala in zimbabwe are maintained in a healthy condition on game farms where they are given supplementary feed during the d ...19902238405
triclabendazole in the treatment of fasciola gigantica infection in swamp buffalo (bubalus bubalis). 19902222372
[inventory of fresh water snails in mayotte, comoro islands].no trematodosis is known at the present time in mayotte despite the close historical relationships between the mayotte archipelago and the neighbouring island of madagascar, which is heavily infested. it is noteworthy that no intermediate host has been described in previous studies in mayotte. an inventory of fresh water snails was performed from 1985 to 1988, in 53 experimental stations during the rainy and the dry seasons: 3,940 snails of eight different species were collected and further iden ...19902092350
characterization of the opisthorchis viverrini genome.the methylations of trematode genomic dna were analyzed using restriction enzymes and southern blot hybridization. restriction enzymes mspi, hpaii, hhai were used to probe cpg methylation while mboi, sau3a, dpni were used for a methylation. the results revealed that opisthorchis viverrini, fasciola gigantica and gigantocotyle siamensis had neither cpg nor a methylations. the presence of highly repeated dna elements was also demonstrated in o. viverrini genomic dna.19912050985
high resistance to experimental infection with fasciola gigantica in javanese thin-tailed sheep.innate resistance of javanese thin-tailed sheep to fasciola gigantica was investigated in animals infected with single doses of 150 or 500 metacercariae and killed 4, 8, 12 or 16 weeks after infection. infected and non-infected sheep had similar values for packed cell volume, mean corpuscular volume, mean corpuscular haemoglobin concentration, serum glutamate dehydrogenase, serum gamma glutamyl transferase and serum aspartate transferase throughout the trial, except for one animal infected with ...19901979191
early diagnosis of human fascioliasis by the detection of copro-antigens using counterimmunoelectrophoresis.we have reported the use of partially purified fasciola gigantica worm antigens (peaks ii and iii separated by sephadex g-200) in counterimmunoelectrophoresis (ciep) for the specific and early detection of antibodies in sera of fascioliasis patients. in the present study, we report the use of hyperimmune serum from rabbits immunized with these partially purified worm antigens in ciep for the detection of parasite antigens in saline extracts of patients' stools. this test was conducted on stool s ...19911949144
[does human distomiasis due to fasiola gigantica exist in the delta of the niger river in mali? (apropos of a serological survey)].finding of specific anti-fasciola antibodies in persons living in the central area of the delta of niger shows that human fascioliasis is a reality in mali. fasciola gigantica, the single species affecting livestock is probably the parasite concerned. we think that this parasitic disease in humans is underestimated, obscured by schistosomiasis which is hyperendemic in the country. complementary studies are much needed for better identification of the problem.19911943637
common antigens of fasciola gigantica, dicrocoelium hospes and schistosoma bovis and their relevance to serology.the possession of common antigens by three trematode parasites which commonly occur together in ruminants in the tropics, fasciola gigantica, dicrocoelium hospes and schistosoma bovis was studied in relation to the reliability of serodiagnosis of infection with these helminths. the crude antigenic extracts of the three trematodes were subjected to sephacryl s-300 column chromatography and f. gigantica was fractionated into six peaks, s. bovis into nine peaks and d. hospes into seven peaks. commo ...19911909064
first report of infectious necrotic hepatitis (black disease) among nubian goats in sudan.in a flock of 425 female and male nubian goats in the khartoum province, an outbreak of a disease causing sudden death of 18 apparently healthy goats occurred (11 females and 7 males, 3-6 years old). adult fasciola gigantica were found in the livers of all goats and in seven of them cysticercus tenuicollis cysts. these organs showed necrotic and severe histopathological changes. clostridium novyi type b was isolated from necrotic areas of all livers and found to be highly pathogenic and toxigeni ...19911824134
problem of fascioliasis in animal husbandry in thailand.one of the most important parasitic diseases in adult cattle and buffalo in thailand is fascioliasis, caused mainly by fasciola gigantica. the economic loss from fascioliasis in cattle and buffalo throughout thailand has been assessed at not less than 100 million baht. recent investigations have been shown that the average prevalence of f. gigantica in cattle and buffalo in thailand was 11.8%. however, the prevalence varies considerably between villages, ranging from 0 to 85%. the prevalence is ...19911822925
partial purification and characterisation of the proteolytic enzymes of fasciola gigantica adult worms.the proteases of adult fasciola gigantica whole worm were analysed by preparative isoelectric focusing and by gelatin-substrate gel electrophoresis at acidic and neutral ph (4.5 and 7.0). at least 15 bands of proteases were observed. these proteases had molecular weights ranging from 26 to 193 kda and isoelectric points of 4.92-7.63. protease-rich fractions were subsequently separated from whole worm preparation of the parasite by filtration in sephacryl s-200. the proteases were able to digest ...19911788929
comparative pathological study of hepatic changes induced by fasciola gigantica and gigantocotyle explanatum in javanese thin-tailed sheep.pathological changes in the liver induced by fasciola gigantica and gigantocotyle explanatum were readily distinguishable from each other. lesions associated with the migration of immature flukes through the parenchyma were a prominent feature of infection with fasciola gigantica, whereas lesions induced by gigantocotyle explanatum were confined to the large bile ducts. the size of the hepatic lesions increased during the course of infection with fasciola gigantica and was associated with the fo ...19911779036
susceptibility to homologous reinfection with fasciola gigantica in goats.goats previously infected with 100 viable fasciola gigantica metacercariae and treated with rafoxanide (ranide, 7.5 mg/kg) at week 4 were not protected against subsequent homologous challenge with 250 metacercariae administered two weeks later. reinfection resulted in more severe hepatic lesions and a higher percentage of flukes recovered as compared with primarily infected controls. however, the size of flukes originating from the second (challenge) infection was considerably reduced. the plasm ...19911775694
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