PMID(sorted descending)
ssu rdna characterization of lymnaeid snails transmitting human fascioliasis in south and central america.the small subunit (18s) rrna gene sequences of the lymnaeid morphs i and ii (mollusca: gastropoda: basommatophora: lymnaeidae) transmitting human fascioliasis in the high endemic zone of the northern bolivian altiplano and of lymnaea cubensis from mexico and guadeloupe island (caribbean) have been obtained by direct polymerase chain reaction pcr cycle sequencing and silver staining methods and compared to that of the 6 most common european lymnaeidae species. results allow us to establish defini ...19979406784
specific monoclonal antibodies to fasciola gigantica.monoclonal antibodies (mabs) were produced against soluble metabolic products (excretory/secretory; es) of the liver fluke, fasciola gigantica. the es antigen was obtained from spent culture medium in which the adult flukes had been maintained in vitro. from two cell fusions, the mabs produces were exclusively associated with either igg or igm isotypes. when screened against a panel of homologous and heterologous parasite antigens by indirect elisa, two mabs were highly specific for f. gigantica ...19979251848
several observations concerning cercarial sheddings of fasciola gigantica from lymnaea natalensis.the shedding of cercariae from lymnaea natalensis and their transformation into metacercariae occurred mainly at night, and 70.7% of the cysts were counted between 7 p.m. and 1 a.m. there is no infradian rhythm in the numerical distribution of the daily mean values as related to experiment duration or patent period duration. one quarter of the snails shed their cercariae in a single wave and the others in 2 to 13 waves. floating cysts represent 35% of the total number of metacercariae and their ...19949235192
the use of monoclonal antibody for the immunodiagnosis of fasciola gigantica infection in cattle.antigens that were specific to fasciola gigantica were obtained from the whole worm homogenate of the parasite by immunoaffinity chromatography in cyanogen bromide-activated sepharose 4b columns and used for the production of monoclonal antibodies. the f. gigantica-specific monoclonal antibody was labelled with horseradish peroxidase and used for the detection of circulating antigen by the direct elisa method in the sera of cattle experimentally infected with the parasite. circulating antigens w ...19979195733
comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with fasciola somatic antigen extract of fasciola gigantica was compared with excretory-secretory (es) antigen in an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of human fascioliasis gigantica. the absorbance values in elisa using the adult somatic antigen were not significantly different from the values obtaining using es antigen (p > 0.05). the diagnostic sensitivity, specificity and positive and negative predictive values of the test using adult somatic extract as antigen were 100%, 9 ...19969185271
the effect of experimental fascioliasis on the pharmacokinetics of antipyrine and sulphadimidine in desert sheep.healthy adult male desert sheep were experimentally infected with fasciola gigantica, to investigate the influence of experimental fasciolasis on the pharmacokinetics of antipyrine and sulphadimidine. each animal received 500 metacercariae orally. the experimental infection was confirmed histologically, by detection of fasciola eggs in faeces and by measuring the activities of the enzymes sorbitol dehydrogenase (sd), glutamate dehydrogenase (gd) and aspartate aminotransferase (ast) in plasma dur ...19979185081
fasciola gigantica and f hepatica: a comparative study of some characteristics of fasciola infection in lymnaea truncatula infected by either of the two trematodes.experimental infections were carried out using three lymnaea truncatula populations and two fasciola species in order to determine the trematode influence on six parameters of snail infection by either of the two trematodes. all experiments were performed using snails 4 mm long, two miracidia for each l truncatula, and a constant temperature of 20 degrees c. no significant influence of trematode species was detected in the following parameters: snail survival at day 30, the life span of infected ...19979112734
evidence for a major gene determining the resistance of indonesian thin tail sheep against fasciola gigantica.the genetic basis of the high resistance of indonesian thin tail (itt) sheep against fasciola gigantica has been studied by measuring the resistance levels of itt sheep, st. croix sheep and their f2 and f3 crossbreds. all of the sheep were bred and reared in the same environment. approximately half of the f2 and f3 crossbreds were as resistant as the itt parental breed, one-quarter had the low resistance of the st. croix parental breed and the rest had an intermediate level of resistance. it is ...19979106952
the isolation of fasciola gigantica-specific antigens and their use in the serodiagnosis of fasciolosis in sheep by the detection of circulating antigens.the 17kda and 69kda polypeptide antigens which are specific to fasciola gigantica were excised from polyacrylamide gels and used for the immunization of rabbits to raise monospecific antisera against these polypeptides. these sera were labelled with horseradish peroxidase and used for the detection of circulating 17 kda and 69 kda antigens by a direct enzyme-linked immunosorbent assay in the sera of sheep that were experimentally or naturally infected with f. gigantica. the 17 kda antigen was de ...19979066072
resistance of indonesian thin tail sheep against fasciola gigantica and f. hepatica.high resistance of indonesian thin tail (itt) sheep against fasciola gigantica has been confirmed. naive itt sheep had only 17% of the number of mature parasites collected from control st. croix sheep. in contrast, the level of resistance of itt sheep against f. hepatica was the same as that of the low resistance merino breed after both primary and secondary infections. it is suggested that resistance of itt sheep against f. gigantica was manifested in two phases. the major phase appeared to be ...19979066053
isolation, cloning, and expression of fatty-acid binding proteins from fasciola gigantica. 19979024206
antibody and circulating antigen profiles before and after chemotherapy in goats infected with fasciola gigantica.the profiles of antibody response and circulating antigen levels in goats infected with fasciola gigantica were studied by enzyme-linked immunoelectrotransfer blot (eitb) and enzyme-linked immunosorbent assay (elisa). in the antibody assay, sera from goats experimentally infected with f. gigantica were reacted with whole worm antigen of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in antibody level 2 weeks after infection. after chemotherap ...19969017880
approaches to the control of fasciolosis in ruminants.approaches to the control of fasciolosis in ruminants are compared for developed countries, and for developing countries with particular reference to regions growing irrigated rice. in all environments problem definition and investigation are based on one or more of observation, abattoir surveys, faecal egg counts, tracer animals and snail studies. in developed countries each husbandman grazes a large number of animals, controls access to pasture and water, and markets high value products. strat ...19968923144
detection of circulating fasciola gigantica antigen in experimental and natural infections of sheep with fasciolosis.detection of circulating fasciola gigantica antigen was performed in sera of sheep with experimental and natural f. gigantica infections using the direct enzyme-linked immunosorbent assay method. sera from sheep with monoinfections of schistosoma bovis, dicrocoelium hospes and paramphistomum microbothrium were included in the assay to ascertain specificity. circulating f. gigantica antigen (cfa) was detected as early as 1 week after infection in the experimentally infected sheep. no detectable c ...19968916398
the efficacy and pharmacokinetics of long-term low-level intraruminal administration of triclabendazole in buffalo with induced fasciolosis.a study was conducted on the pharmacokinetics and therapeutic efficacy of triclabendazole at three low dose rates of 0.5, 1.0 and 1.5 mg/kg body weight in buffaloes experimentally infected with fasciola gigantica. the pharmacokinetics were compared with the effects of a single intraruminal dose at 24.0 mg/kg body weight in uninfected buffaloes. at all three dose rates, an equilibrium between the absorption of triclabendazole and the disposition of its metabolites was observed by days 3 and 4 and ...19968908726
acquired resistance of merino sheep against fasciola gigantica.merino sheep acquired resistance against fasciola gigantica, which is contrary to previous observations of infections with f. hepatica in that breed. the acquired resistance was manifest against young adult parasites. st. croix sheep had previously been shown to have more resistance than european sheep against f. hepatica after primary infection; however, in f. gigantica infections in the present study there was no difference between the resistance levels of the breeds. antigenic analysis of the ...19968897512
seroepidemiological survey of taenia saginata cysticercosis in kenya.a sero-epidemiological study of taenia saginata cysticercosis was carried out to determine the prevalence and distribution of the infection in three provinces of kenya. serum samples and meat inspection records were collected from cattle at slaughter at export and district abattoirs. cattle origin and the presence of t. saginata cysticerci were noted as was the prevalence of other helminths such as echinococcus granulosus and fasciola gigantica. serum samples were screened for circulating parasi ...19968888551
studies on helminthosis at the centre for tropical veterinary medicine (ctvm).the research work relating to helminths, which has been conducted within the helminthology section of the ctvm, often in collaboration with colleagues from the tropics is reviewed and placed into a historical perspective. the research has, in the main, concentrated on the trematodes fasciola hepatica and fasciola gigantica and the cestodes taenia saginata and taenia solium, but work on other parasites including gastro-intestinal nematodes is also considered. all of these parasites are of obvious ...19968815611
efficacy and pharmacokinetics of triclabendazole in buffalo with induced fasciolosis.a study was conducted to understand pharmacokinetics and flukicidal activity of intraruminal administration of triclabendazole (tcbz) at 12.0, 24.0 and 36.0 mg kg-1 body weight in experimentally fasciola gigantica-infected buffaloes on week 2 and 10 post-infection. no fluke eggs in faeces and no flukes could be recovered from the liver of buffaloes following intraruminal administration of triclabendazole at 24.0 and 36.0 mg kg-1 body weight both on weeks 2 and 10 post-infection, while the recomm ...19968792582
immunodiagnostic moieties in somatic and excretory/secretory antigens of fasciola gigantica.immunodiagnostic potential fractions in fluke antigens and excretory/secretory (e/s) antigen of f. gigantica have been identified using double immunodiffusion (did), immunoelectrophoresis (iep) and sodium dodecyl sulphate (sds) page electrophoresis. hyperimmunised rabbits elicited considerable level of antibody response with few precipitin lines showing reaction of identity in did suggesting presence of some common fractions in all antigens. mature fluke somatic (mfsag) and immature fluke somati ...19958786154
fasciola gigantica in the common bile duct: diagnosis by ercp. 19968781802
[paramphostomid infestation in equids in egypt].the investigation of 156 donkeys in the area of the veterinary faculty of the tanta university in kafr el-sheikh showed that 49 animals (31.41%) were infected with trematodes, 34 donkeys (21.80%) served as hosts for gastrodiscus aegyptiacus, 22 (14.10%) were infected with fasciola gigantica und 7 (4.49%) with both trematodes. 6 of 40 examined horses (15.0%) showed monoinfections with g. aegyptiacus only. because of the fragmentary knowledge on these paramphistomides of equids, some aspects conce ...19968765538
helminth parasites and hypobiosis of nematodes in n'dama cattle during the dry season in the gambia.three series of necropsies of cattle were performed, corresponding to early dry season, approximately 1 month after the last rains (november, n = 6), mid dry season (february, n = 6) and end dry season (april, n = 3). eggs per gram of faeces (epg) were determined just before necropsy. three trematodes (fasciola gigantica, schistosoma spp. and paramphistomatids) and 11 nematodes were identified from cattle, with the prevalence rate varying from 6.7% to 100%. haemonchus contortus was the most abun ...19958644452
prevalence of hepatic helminths and associated pathology in impala (aepyceros melampus) in swaziland.the livers of impala (aepyceros melampus) collected in the mlawula-mbuluzi-simunye nature reserve and protected area complex in northeastern swaziland from october 1985 to september 1986 were infected with three species of helminths: a nematode (cooperioides hepaticae), a cestode (stilesia hepatica), and a trematode (fasciola gigantica). cooperioides hepaticae caused a severe cholangitis. lambs were infected at 1.5 to 2 mo of age, and the prevalence increased to 100% by 5 mo of age. the prevalen ...19968627927
evaluation of fasciola excretory-secretory (e/s) product in diagnosis of acute human fasciolosis by igm elisa.diagnosis of fasciolosis in the acute phase depends on a sensitive and accurate serological test. the present study is an evaluation of the efficacy of excretory-secretory fasciola gigantica adult worm antigen by igm elisa. thirty eight patients with acute fasciolosis and 14 in the chronic phase together with 23 patients with different parasitic infections were introduced in the study. seventeen healthy, parasite free individuals, were served as controls. a crude excretory-secretory antigen and ...19958525282
cytochemical localization of cytochrome c oxidase activity in mitochondria in the tegument and tegumental and parenchymal cells of the trematodes echinostoma trivolvis, zygocotyle lunata, schistosoma mansoni, fasciola gigantica and paragonimus ohirai.cytochrome c oxidase in the mitochondria of the tegument and tegumental and parenchymal cells was examined cytochemically in echinostoma trivolvis, zygocotyle lunata, schistosoma mansoni, fasciola gigantica and paragonimus ohirai, trematodes that inhabit different sites in their vertebrate hosts. clear differences in enzyme activity occurred in the mitochondria of these species, probably reflecting the different energy metabolisms of these worms. marked aerobic metabolism occurred in s. mansoni ...19958522764
distribution of antigens of fasciola gigantica at various stages of indirect immunofluorescent antibody test on the serum of a fasciola gigantica-infected rabbit, showed specific fluorescence on the tegumental surface of miracidia, sporocysts, rediae, cercariae, metacercariae and juvenile worms aged between one and seven weeks. the same reaction occurred in the gut cells of cercariae and metacercariae and in the gut caeca of juveniles in the liver. the results of the absorption test indicated that stage-specific antigen(s) in metacercariae and seven-week-old ...19938460268
banding patterns of fasciola hepatica and fasciola gigantica (trematoda) by isoelectric focusing.liver flukes fasciola hepatica and fasciola gigantica are polymorphic and vary morphologically depending upon the host being parasitized. it is known also that mixed infection occurs where both species are present. a technique involving protein separation was used to distinguish the 2 species. isoelectric focusing of soluble proteins was performed on polyacrylamide gels using whole-body proteins from adult flukes. although many bands appeared common to both species and some were shared with host ...19938437051
the protective role of fasciola tegument antigen against trichinella spiralis infection.fasciola gigantica tegument antigen was prepared to be used for induction of immunity against trichinella spiralis in albino mice. the results of the study showed that the antigen was more effective when administered in a dose of 3 micrograms than in a dose of 30 micrograms. this was evidenced by the reduction in adult count 72%, adult length 61.4%, larval count 78.7%, length of muscle larvae 32.2% and the significant reduction in reproductive capacity index.19938376868
comparison of the second internal transcribed spacer (ribosomal dna) from populations and species of fasciolidae (digenea).the nucleotide sequence of the 3' end of the second internal transcribed spacer region (its2) was determined for four isolates of fasciola hepatica, two isolates of fasciola gigantica, one isolate of fascioloides magna and one isolate of fasciola sp. from japan. intraspecific sequence divergence was negligible. sequence divergence between f. hepatica and f. gigantica was 2.8% and between fasciola hepatica and fascioloides magna was 13.2%. the sequence of fasciola sp. from japan matched closely t ...19938359995
effects of ration and fasciola infection on some blood parameters of ewes.this study was conducted using fourty cross-bred ewes in 5 groups of which one was kept as a control and the others were infected with metacercariae of fasciola gigantica twice at the beginning of the experiment and 200 days from the first. the four infected groups fed on basal ration or high protein ration with or without mineral mixture. blood samples were taken from the five groups to assess the concentrations of plasma fibrinogen and serum total proteins, albumin, globulin, calcium and inorg ...19938308350
schistosomiasis and intestinal parasites in rural villages in southwest nigeria: an indication for expanded programme on drug distribution and integrated control programme in nigeria.a cross sectional survey of schistosomiasis, intestinal parasite infections and pattern of schistosomiasis transmission was undertaken in four rural villages around the erinle dam, nigeria. the prevalence of schistosoma haematobium ranged from 10% to 60%. 30% of infected school children excreted over 50 eggs/10 ml urine and high rates of haematuria, proteinuria, leucocyturia and nitrites in urine were observed in infected children and the villagers. prevalence of infection with s. mansoni ranged ...19938256092
liver fluke infections in livestock in borno state, nigeria.a survey was conducted on the prevalence of fasciola gigantica and dicrocoelium hospes eggs in gall bladders of cattle and small ruminants (sheep and goats) slaughtered between november 1987 and december 1988 in maiduguri, borno state, nigeria. prevalence rates of 42.7% and 5.7% were recorded for f. gigantica and d. hospes eggs, respectively, in 246 cattle and 718 sheep and goats examined. out of the 246 cattle examined, 52 (21.1%), 45 (18.3%) and 8 (3.3%) excreted eggs of f. gigantica, d. hospe ...19938122358
cloning and sequence analysis of the major cysteine protease expressed in the trematode parasite fasciola sp.a cdna encoding the fasciola cysteine protease precursor has been cloned and sequenced. the deduced precursor contains 325 amino acid residues (m(r) 36,768) consisting of a signal sequence (pre-region, 15 residues), pro-region (90 residues), and mature protease (220 residues, m(r) 24,371). cys27, his164, and asn184 form the catalytic triad in the active site of the mature enzyme, and their adjacent regions are highly conserved. the parasite enzyme showed 50, 44, and 27% amino acid sequence homol ...19938118429
efficacy of triclabendazole against fasciola gigantica in buffaloes in eastern nepal. 19947886900
serodifferentiation of human fascioliasis from schistosomiasis.a dot enzyme-linked immunosorbent assay (dot-elisa) was developed using excretory-secretory (es) antigens of fasciola gigantica adult worms and freeze-thaw antigen (aft) of adult schistosoma mansoni worms. specific igg and igm antibodies were simultaneously investigated in sera from 17 febrile eosinophilic hospitalized patients and 20 healthy controls. dot-elisa was shown useful and could differentiate fascioliasis from schistosomiasis prior to parasite egg detection in patient's stool. this ass ...19947855923
molecular weight determination of fasciola antigens specific for diagnosis of acute fascioliasis.acute human fascioliasis depends in its diagnosis on detecting specific antibodies in the sera of patients. it was observed that both somatic fraction i and crude e/s products of adult fasciola gigantica worm gave sensitive and specific results. using igm elisa, the cut off o.d was 0.6 and 0.3 nm. and the accuracy was 93% and 98% for the two antigens respectively. determination of the molecular weight of these two antigens was undertaken by sds-page. the molecular weight of the somatic fraction ...19947844411
partial characterization of larval antigens of strongyloides papillosus by western blot analysis.western blotting was used to analyse the antigens in extracts of adult and infective larvae (sil) of strongyloides papillosus with serum of infected rabbits. several proteins in both the extracts reacted with immunoglobulin g in the serum. a protein of about 16 kda in the extract of sil showed an especially strong reaction. no cross-reaction was observed among proteins in the extracts of adult worms of such cattle helminths as fasciola gigantica, homalogaster paloniae, trichuris discolor and set ...19947812315
biomphalaria alexandrina naturally infected with fasciola gigantica in egypt. 19957747303
[epidemiology of gastrointestinal helminthiasis in small ruminants in the tree-cropping pasture zone in senegal].an epidemiological survey on gastrointestinal helminthiasis in 51 sheep and 51 goats was held in the tree-cropping pasture region in senegal from october 1990 to september 1991. all the animals examined were infected with at least 1 helminth species. three trematodes (fasciola gigantica, schistosoma bovis, amphistomatids), 2 cestodes (moniezia expansa, cysticercus tenuicollis) and 9 nematodes were identified. the most important parasite in sheep was haemonchus contortus while trichostrongylus co ...19957735304
[immunodiagnosis of fasciola gigantica fascioliasis by elisa technique in senegal. preliminary observations in 2 lambs].the purpose of this work was to investigate the use of the elisa test for the diagnosis of fasciolosis (fasciola gigantica) in sheep, in senegal, using fasciola gigantica metabolic excretion-secretion products as antigens. coprological analysis results were negative until the fourteenth week following infection; using the elisa test, the first anti-fasciola gigantica antibodies can be detected as early as the fourth week following infection.19947709030
development and characterization of a monoclonal antibody reactive with a 28 kda protease of fasciola gigantica.a monoclonal antibody that was reactive with a 28-kda protease of fasciola gigantica was produced after hyperimmunizing balb/c mice with a partially purified extract and a purified 28-kda protease obtained from the fluke. isotype analysis showed that the immunoglobulin is igg3. seven other monoclonals of isotypes igg1, igg2 and igg3 which reacted with the protease and one to three other protein bands were also produced. in addition, four other clones of hybridoma produced antibodies which recogn ...19957660572
immunodiagnosis of fasciolosis in ruminants using a 28-kda cysteine protease of fasciola gigantica adult worms.a homogeneous extract of a 28-kda cysteine protease of fasciola gigantica adult worms was used as the antigen for immunodiagnosis of fasciolosis in cattle, sheep and goats using the falcon assay screening test enzyme-linked immunosorbent assay technique. this antiprotease assay technique was found to be very rapid and sensitive and may be useful as a supplementary method for the diagnosis of fasciolosis in ruminants.19957660568
separation of outer cysts from metacercariae of fasciola gigantica in an aqueous two-phase system.the outer cysts (oc) of fasciola gigantica metacercariae (mc) were separated in an aqueous two-phase system composed of polyethylene glycol and dextran in distilled water. a large number of separated mc without oc were recovered in the bottom layer rich in dextran in the system. ten separated mc in the layer were used to inoculate the mouse with a syringe and juveniles were recovered from the animals on the 14th day. when the number of juveniles recovered was compared with that from the mice inf ...19957622796
time-course analysis of antibody response by eitb and elisa before and after chemotherapy in sheep infected with fasciola gigantica.the time-course analysis of the antibody response to fascicola gigantica infection in sheep was studied by enzyme-linked immunosorbent assay (elisa) and enzyme-linked immunoelectrotransfer blot (eitb). sera from sheep experimentally infected with f. gigantica were reacted with excretory-secretory antigens of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in anti-fasciola antibody by 2 weeks after infection and there was a sharp decrease in an ...19957571329
detection of circulating antigen in sera of fasciola gigantica-infected cattle with antibodies reactive with a fasciola-specific 88-kda antigen.antibodies against a specific 88-kda antigen of fasciola gigantica were used for the detection of circulating antigen in the sera of cattle with experimental and natural infections of f. gigantica by a double antibody elisa. circulating antigen was detectable as early as the second and third weeks after infection and positive absorbance values were obtained for the entire duration of infection. absorbance values decreased below the cutoff point 3 weeks after chemotherapy with oxyclozanide. this ...19957571328
comparative studies on the productivity of fasciola gigantica and f. hepatica sporocysts in lymnaea tomentosa that died after a cercarial shedding or without emission.a histology study was performed on fasciola gigantica- or f. hepatica-infected lymnaea tomentosa that died after a cercarial shedding or without emission to compare the parasite productivity of each trematode. degenerated rediae increased in number throughout the experiment. their number rose rapidly after day 79 in snails that died after shedding in the f. gigantica group; they increased more slowly in snails that died without shedding. in the f. hepatica group the number rose after day 63 in t ...19957567914
malumfashi endemic diseases research project xii. schistosoma bovis and fasciola gigantica in livestock. 19807458459
extermination of fasciola gigantica metacercariae. 19807420254
incidence of liver fluke infections (fasciola gigantica and dicrocoelium hospes) in ruminants in northern thousand and twenty-four cattle, 550 sheep and 1,748 goats slaughtered in a rural slaughter slab during 1973 to 1975 were examined for evidence of liverfluke infections. the prevalence rate of fasciola gigantica and dicrocoelium hospes infections was respectively 65.4% and 56.0% in cattle, 40.8 and 13.1% in sheep and 17.6 and 5.2% in goats. other trematodes detected were schistosoma bovis and paramphistomes. the seasonal incidence of f. gigantica as well as of d. hospes was highest during an ...19807414705
carbohydrate energy metabolism in fasciola gigantica (trematoda). 19807409972
the effect of gamma-irradiation on fasciola gigantica infectivity to hamsters. 19807364501
cyclophosphamide as a potential chemosterilant for harmful snails.cyclophosphamide induced changes in the ovotestis of the snail lymnaea acuminata, the vector of the giant liverflukes fasciola hepatica and fasciola gigantica were studied in order to explore potential of the drug as a chemosterilant for snails. the drug caused a dose dependent reduction in the levels of dna, rna and proteins and the activity of the enzyme alkaline phosphatase. it increased the activity of acid phosphatase and the levels of total free amino acids in the ovotestis. while the anim ...19817336977
red blood cell survival and faecal clearance in sheep infected with fasciola gigantica. 19817320242
in vitro effect of nitroxynil (trodax) and rafoxanide on adult fasciola gigantica. 19817313227
a method for laboratory maintenance of lymnaea natalensis and for mass production of fasciola gigantica metacercariae. 19817299589
the effect of five fasciolicides on malate dehydrogenase activity and mortality of fasciola gigantica, fasciolopsis buski and paramphistomum explanatum.the effect of oxyclozanide, hexachlorophene, nitroxynil, rafoxanide and diamphenethide on malate dehydrogenase activity of homogenates of fasciola gigantica, fasciolopsis buski and paramphistomum explanatum was investigated. the ratio of oxaloacetate reduction to malate oxidation in homogenates of fasciola gigantica, fasciolopsis buski and p. explanatum was 4.5:1, 3.6:1 and 5.2:1 respectively. oxyclozanide and rafoxanide at 10(-3) m inhibited enzyme activity by 100% in homogenates from all three ...19817264272
the epidemiology of fascioliasis in malawi. part ii. epidemiology in the definitive host.the epidemiology of fasciola gigantica cobbold was studied in the definitive host and the epidemiological cycle was determined. the incidence of immature flukes was high in september to december and mature flukes from december to march. a survey of chronic fascioliasis was conducted covering almost the whole country. the prevalence of the disease was high in the northern and central regions. based on the epidemiological findings a control programme for the disease in malawi was prepared.19817256868
[the large island--impressions of a medical parasitological trip to madagascar].the course of an investigation trip through madagascar is sketched. it allowed to analyze the parasitological situation. it is explained with the sociological and economical facts and the unusual fauna of this island. the two biggest problems are malaria tropica and schistosomatosis (bilharziasis). further, ancylostoma, ascaris, taeniids, and wuchereria occur. plague is rare. fasciola gigantica has been introduced since short time only. introduction of other parasites must be feared. sleeping il ...19817235291
susceptibility of different species of lymnaea snails to miracidia of fasciola gigantica and f. hepatica in iran. 19807217650
[epidemiologic study of s. haematobium bilharziasis in the rice fields of yagoua (north cameroon) ii. distribution and ecology of intermediate hosts].while inventoring the local malacological fauna and evaluating the density of species acting as intermediate hosts of human schistosomes it has been shown that bulinus truncatus is predominant in the study area. bulinus globosus is scarcely encountered. biomphalaria pfeifferi is only found in the mayo guerleo, in irrigation canals and draining ditches. but b. globosus and b. pfeifferi have not yet invaded the recent aquatic network and are not found in the lake nor in the newly created irrigatio ...19827201892
effect of sodium benzene sulphonate on adults and eggs of lymnaea natalensis and miracidia of fasciola gigantica. 19827199774
studies on specific inhibitors and substrates of cholinesterases of fasciola gigantica from cattle and buffaloes. 19827180251
alpha esterase enzyme in liver of rabbits infected with fasciola gigantica and treated with bithionol. 19827153561
changes in blood cellular components, serum protein concentrations and serum enzyme activities in buffaloes infested with fasciola gigantica.of 510 adult buffaloes examined, 88 (17.3 per cent) were found to be suffering from fasciola gigantica infestation. there was a reduction in the haemoglobin, packed cell volume and red blood cell count in the fasciola affected buffaloes and an increase in their white blood cell count. there was no significant change in mean corpuscular volume, mean corpuscular haemoglobin or mean corpuscular haemoglobin concentration in the fasciola affected buffaloes. there was also a decrease in total serum pr ...19827146634
bovine fascioliasis in nigeria--intercurrent parasitic and bacterial infections.the bacterial and parasitic infections associated with field cases of bovine fascioliasis due to fasciola gigantica were investigated; 47.6% of infected cattle had blood parasites, 61.9% had faecal-borne parasites and 85.8% had positive bacterial isolates in the bile in contrast to 13.3% with blood parasites, 71.1% with faecal-borne parasites and 28.9% positive for bile bacteria among uninfected cattle. trypanosoma spp., babesia, paramphistomum and schistosoma were the most commonly found parasi ...19827101464
specific inhibitors and substrates studies on the cholinesterases of fasciola gigantica from sheep and goats. 19836871931
activation of prophenol oxidase in the liver fluke fasciola gigantica cobbold.activation of prophenol oxidase of fasciola gigantica has been demonstrated using biochemical and electrophoretic techniques. based on the difference in the electrophoretic pattern of the proenzyme and activated enzyme, a probable mechanism of activation of the prophenol oxidase is suggested. a role for mehlis' gland in the activation process is suggested. the significance of the results is discussed in the light of related studies in insects.19826817288
igg response in natural and experimental infections of cattle with fasciola gigantica.a specific igg response was detectable in serum and bile of cattle infected with fasciola gigantica. although there was an increase in both specific and total igg concentration in the sera of fluke-infected cattle, there was no significant correlation between igg1 concentration and the worm burden (r = 0.17; p greater than 0.05) in chronic fascioliasis. however, there was a highly significant correlation between the levels of igg1 in the serum and bile of infected cattle (r = 0.87; p less than 0 ...19836686385
[epidemiology of hepatic fascioliasis (fasciola gigantica) at the dairy farm of the lubumbashi (zaire) ranch. results of a deparasitization trial. recommendations]. 19836672883
effect of bithionol on b-glucuronidase in serum and liver of rabbits infected with fasciola gigantica. 19836631102
clinical diagnosis of schistosomiasis in sudanese cattle.this study was done in the white nile province to characterise the history and signs of naturally occurring schistosoma bovis infection in cattle (gorag). necropsy and laboratory examinations were performed on 10 animals six to 30 months of age which were in poor condition. they were selected because of a history suggestive of schistosomiasis. all the animals showed some degree of s. bovis infection; eight had a moderate or heavy degree of infection. also all had liver damage due to either past ...19836623642
efficacy of ivermectin against internal parasites of sheep.ivermectin in an oral formulation was evaluated by the non-parametric method against a wide range of endoparasites of sheep in 15 trials involving a total of 297 sheep. ivermectin at 200 micrograms/kg was more than 80% effective in more than 80% of the treated animals (i.e. class a) against induced infestations of 3rd and 4th stage larvae and adults of chabertia ovina, dictyocaulus filaria, haemonchus contortus (including a benzimidazole-resistant strain), nematodirus spathiger, oesophagostomum ...19846549472
the effect of sex on seasonal variation in single and double infection of cattle in sierra leone by dicrocoelium hospes and fasciola gigantica.the incidence of dicrocoeliasis (61.8%) is higher than that of fascioliasis (25.5%) and their peaks do not occur at the same time of the year. the peak for the former occurs in the dry season while that of the latter occurs in the rainy season. the incidence of dicrocoeliasis and fascioliasis in female cattle was found to be higher than that of male cattle. double infection was higher in female (14.7%) than in male hosts (9.6%).19846541838
biology, life-tables, and intrinsic rates of increase of bulinus globosus morelet and lymnea natalensis kraus (gastropoda: pulmonata)--the snail intermediate hosts of schistosoma haematobium and fasciola gigantica, respectively, in southeastern nigeria.two tables and a figure are used to present the epidemiological and epizoological particulars of bulinus globosus and lymnaea natalensis, the intermediate hosts of schistosoma haematobium and fasciola gigantica in southeastern nigeria. possible ways of reducing the populations of these intermediate hosts are seen in the combined application of chemical agents and environmental shaping.19846529436
infectivity and pathogenicity of fasciola gigantica in west african dwarf sheep and goats.the infectivity and pathogenicity of fasciola gigantica for sheep and goats were investigated in animals infected with single doses of between 200 and 2,000 metacercariae. acute fascioliasis occurred in sheep infected with 1,000 to 2,000 cysts in which 10 to 17% of the cysts became established. the infected animals died 80 to 90 days after infection. in goats given the same infective doses death from acute fascioliasis occurred earlier (70 to 83 days after infection) and 12 to 38% of cysts becam ...19846485107
use of a partially purified fasciola gigantica worm antigen in the serological diagnosis of human fascioliasis in egypt.crude extracts of fasciola gigantica adult worms, when used as an antigen in indirect hemagglutination (iha) and counterimmunoelectrophoresis (ciep) tests, detected all independently diagnosed human f. gigantica and f. hepatica infections but cross-reacted with sera of patients with schistosomiasis and amebiasis. fractionation of this crude worm extract using sephadex g-200 chromatography demonstrated four major protein peaks. antigen from the crest and descending portion of peak ii (mol. wt. ap ...19836305221
study of phosphagens in two trematodes.extracts from the adults of two trematodes fasciola gigantica and paramphistomum explanatum have been shown to contain three phosphagens, phosphoarginine, phosphocreatine, and phosphoglycocyamine. the three phosphokinases involved in the transphosphorylation of these phosphagens in both worms were inhibited by edta and mercuric ions while iodoacetate had no effect.19806245542
chemosterilization and its reversal in the snail lymnaea acuminata.treatment of the snail lymnaea acuminata, the vector for the liver flukes fasciola hepatica and fasciola gigantica, with two alkylating agents (cyclophosphamide and busulfan) and one thiocarbamide (thiourea) caused reduction in the number of eggs and production of non-viable embryos. all the three drugs caused reduction in the levels of dna, rna and protein and the activity of alkaline phosphatase in the gonadal tissues of this snail. there was enhancement in free amino acid levels and activity ...19836189361
[additional data on superficial argentophilic structures of the miracidium and cercaria of fasciola gigantica (cobbold, 1855) (trematoda:fasciolidae) and on the epidemiology of this parasite].the life cycle of a senegalese strain of fasciola gigantica was carried out in france from adults recovered in the liver of naturally infected cattle (bos taurus). laboratory-reared lymnaea natalensis experimentally infected shed numerous cercariae. metacercariae were used for successful infection of sheep. in the miracidium, variations are observed in the disposition of the medio-ventral epithelial cell of the second tier. the distribution of the superficial argentophilic structures is similar ...19806164333
the enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths.the activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in crithidia fasciculata, trypanosoma cruzi, leishmania major, trichomonas vaginalis, eimeria tenella, toxoplasma gondii, plasmodium berghei, fasciola gigantica, schistosoma mansoni, hymenolepis diminuta, nippostrongylus brasiliensis and trichuris muris. the m ...19816111750
dynamics of lymnaea natalensis populations in the zaria area (nigeria) and the relation to fasciola gigantica infections.observations on the seasonal changes in the lymnaea natalensis population and the infection rate of these snails with fasciola gigantica during 1972-1976 are reported. the data were obtained from snails in three different locations near zaria in northern nigeria. one of the survey sites dried out during the drought of 1972-1974 and had to be abandoned. the snail population showed seasonal variations with the lowest number of snails at the very end of the dry season. many juvenile snails develope ...19806106353
[case of infection with fasciola gigantica cobbold, 1856]. 19676063274
comparison of hemoglobins of fasciola gigantica (trematoda: digenea) and its host. 19676026225
a note on dicrocoeliasis and fasciola gigantica infection in livestock in northern ghana, with a record of spurious and of genuine dicrocoelium hospes infections in man. 19666006926
on the comparative susceptibility of lymnaea natalensis (kraus) and l. rufescens (gray) to infection with fasciola gigantica (west african strain) and the tissue responses in the snails. 19665961523
osmotic and ionic regulation in fasciola gigantica (trematoda: digenea). 19665960789
[preliminary note on the presence of fasciola gigantica in madagascar]. 19665951730
the occurrence of fasciola gigantica in the lungs of indian buffaloes (bos bubalis). 19655890486
effect of the seasonal variations of temperature on the development of fasciola gigantica in the snail host in the kenya highlands. 19635889258
histopathological changes in the liver of a spotted deer (axis axis) infected with fasciola gigantica. 19695813688
[3 cases of hepatic fasciolasis due to fasciola gigantica contracted in central africa]. 19685759965
suitability of extracts of fasciola gigantica for diagnosis of schistosomal cases. 19685736436
3. fractionation of the antigen of fasciola gigantica by electrophoresis and the use of the fractions in the intradermal test for diagnosing bilharziasis. 19685726943
ii. fractionation of the antigen of fasciola gigantica by salting out with reference to the use of the isolated fractions as skin test antigens in cases of urinary schistosomiasis. 19685726942
[some data on comparative biochemical and immunological studies of the number of antigenic preparations from fasciola hepatica and fasciola gigantica]. 19685712600
i. protein and polysaccharide content of fasciola gigantica skin test antigens and their reactivity in the diagnosis of bilharziasis. 19685702063
[the effect of bilevon m against immature and mature stages of fasciola gigantica in artificially infected sheep]. 19685693411
[effectiveness of zanil against immature and sexually mature stages of fasciola gigantica in artifically infected sheep]. 19685692951
[hepatic distomatosis due to fasciola gigantica (tropical fluke). importance of psychic problems]. 19685688554
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