PMID(sorted ascending)
characterization of a fasciola gigantica protein carrying two dm9 domains reveals cellular relocalization property.even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. classified among the proteins of unknown function is a family of proteins harboring repeats of the dm9 domain, a 60-75 amino acids motif first described in a small number of drosophila melanogaster proteins. proteins may carry two or more dm9 domains either in combination with other domains or as their sole constituent. here we have charact ...201626946400
fasciola gigantica enolase is a major component of worm tegumental fraction protective against sheep fasciolosis.infection of cattle and sheep with the parasite fasciola gigantica is a cause of important economic losses throughout asia and africa. many of the available anthelmintics have undesirable side effects, and the parasite may acquire drug resistance as a result of mass and repeated treatments of livestock. accordingly, the need for developing a vaccine is evident. triton-soluble surface membrane and tegumental proteins (tsmtp) of 60, 32, and 28 kda previously shown to elicit protective immunity in ...201626970372
cross antigenicity of immunodominant polypeptides of somatic antigen of oesophagostomum columbianum with other helminths by western blotting.oesophagostomum columbianum in small ruminants in india is found as mixed infection commonly in sheep and goat. haemonchus contortus, an abomasal nematode is found as concurrent infection with it. eggs of haemonchus and o. columbianum cannot be easily distinguished. diagnosis of o. columbianum may only be possible if a non-cross antigenic polypeptide was available for immunodiagnosis.201527047030
staining of platyhelminthes by herbal dyes: an eco-friendly technique for the environment compatible technique to stain platyhelminthes, fasciola gigantica, gastrothylax crumenifer, taenia solium, and moniezia expansa using aqueous and alcoholic extract of sugar beet (beta vulgaris), china rose (hibiscus rosa-sinensis), and red rose (rosa hybrida) were described to minimized the deleterious effects of the synthetic dyes.201527047037
the role of ser-(arg-ser-arg-ser-glucnac)19-glucnac fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in the present study, the carbohydrate structures associated with fasciola gigantica adult worm were identified by indirect hemagglutination inhibition test. glucose was found to be the main monosaccharide associated with the fluke. according to indirect hemagglutination inhibition results, purification of glycoprotein fractions from worm crude extract was carried out by affinity chromatography immobilized glucose agarose gel and con-a lectin columns. the isolated glycoprotein fractions, fi and ...201627065591
in vitro tegumental alterations on adult fasciola gigantica caused by mefloquine.emergence of drug-resistant fasciola strains has drawn the attention of many authors to alternative drugs. the purpose of this study is to explore the in vitro effect of the antimalarial mefloquine against adult fasciola gigantica. light and scanning electron microscopic observations could be used to determine the target of the drug following 6 and 12 h of incubation in medium containing mefloquine at three different concentrations 10, 20 and 30 μg/ml, as morphological changes could be observed. ...201627065615
development of fasciola vaccine in an animal model.fasciola hepatica and f. gigantica are the parasites that cause the zoonotic parasitic disease called fasciolosis. although several anthelmintic drugs have been used to treat these parasitic infections, recombinant protein vaccines have been developed to overcome the anthelmintic resistance that has recently been reported in many regions of the world. these vaccines have been shown to induce high levels of immune responses and high percentages of protection in experimental and large animals. eff ...201627076294
cias detection of fasciola hepatica/f. gigantica intermediate forms in bovines from bangladesh.fascioliasis is an important food-borne parasitic zoonosis caused by two trematode species, fasciola hepatica and fasciola gigantica. the characterisation and differentiation of fasciola populations is crucial to control the disease, given the different transmission, epidemiology and pathology characteristics of the two species. lineal biometric features of adult liver flukes infecting livestock have been studied to characterise and discriminate fasciolids from bangladesh. an accurate analysis w ...201627078650
fasciola gigantica thioredoxin glutathione reductase: biochemical properties and structural modeling.platyhelminth thioredoxin glutathione reductase (tgr) is a multifunctional enzyme that crosstalk between the conventional thioredoxin (trx) and glutathione (gsh) system. it has been validated as a potential drug target in blood flukes. in the present study, we have performed a biochemical study on fasciola gigantica tgr with substrates dtnb and gssg. the michaelis constant (km) with dtnb was found to be 4.34±0.12μm while it was 61.15±1.50μm with gssg. the kinetic results were compared with the t ...201627112978
antigenic profile, isolation and characterization of whole body extract of paramphistomum antigenic component of adult paramphistomum gracile was characterized by means of indirect enzyme-linked immunosorbent assay (indirect elisa), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting using sera from cattle naturally infected with p. gracile, eurytrema pancreaticum, fasciola gigantica, moniezia benedeni, strongylids, trichuris sp. and strongyloides sp. the whole body (wb) extracts of p. gracile were fractionated by gel filtration chromatography ...201627135198
purification and characterization of two-domain glutaredoxin in the parasitic helminth fasciola gigantica.glutaredoxins (grxs) are small thiol-dependent proteins and key elements of redox signaling as they regulate the redox state of important cellular proteins. in the present study, the complete sequence of a glutaredoxin protein, obtained from the liver fluke fasciola gigantica, was pcr-amplified and cloned. the 690-bp open reading frame (orf) encodes a 230-amino acid protein with two conserved domains (fggrxd1 and fggrxd2) and has similarities with two monothiol grxs of saccharomyces cerevisiae, ...201627189489
use of bait containing triclabendazole against fasciola gigantica in a herd of captive wild impala (aepyceros melampus).although the efficacy of triclabendazole treatment against fasciola gigantica has been shown before in cattle and buffaloes, there appears to be no published report on the efficacy of triclabendazole in impala or other antelope species. as part of a health monitoring programme at mulungushi international conference centre, lusaka, zambia, a coprological examination was undertaken to investigate the helminthological status of captive impala (aepyceros melampus). of 39 fresh coprological samples, ...201727225321
anthelmintic activity of chlorophyllin against different larval stages of fasciola gigantica.fasciolosis is a food borne zoonosis, caused by the digenetic trematode fasciola. freshwater lymnaeid snails are the intermediate host of the trematodes. chlorophyllin, a semi-synthetic derivative of chlorophyll and its formulations obtained from freeze dried cow urine (fcu) had their toxicity tested against redia and cercaria larvae of f. gigantica. the larvicidal activity of chlorophyllin and its formulations were found to depend on both, time and concentration used against the larvae. toxicit ...201627253741
molecular characterization and phylogenetic analysis of fasciola gigantica from western java, indonesia.fasciola gigantica and aspermic (hybrid) fasciola flukes are thought to be distributed in southeast asian countries. the objectives of this study were to investigate the distribution of these flukes from unidentified ruminants in western java, indonesia, and to determine their distribution history into the area. sixty fasciola flukes from western java were identified as f. gigantica based on the nucleotide sequences of the nuclear phosphoenolpyruvate carboxykinase (pepck) and dna polymerase delt ...201627266482
molecular characterization of fasciola gigantica in delhi, india and its phylogenetic relation to the species from south asian countries.the aim of this study was to phylogenetically analyze fasciola gigantica (f. gigantica) from mainland india and to reveal the expansion history of f. gigantica in the indian subcontinent. we analyzed 40 fasciola flukes that were collected from delhi, in the indian mainland, and identified them as f. gigantica by using nucleotide analyses of the nuclear phosphoenolpyruvate carboxykinase (pepck) and dna polymerase delta (pold) genes. based on the nucleotide sequence of mitochondrial nadh dehydroge ...201627301703
immunodiagnostic monoclonal antibody-based sandwich elisa of fasciolosis by detection of fasciola gigantica circulating fatty acid binding protein.up to now, parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. hence, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden. in the present study, a monoclonal antibody (moab) against recombinant fasciola gigantica fatty acid binding protein (rfgfabp) has been produced. as well, a reliable sandwich enzyme-linked immunosorbent assay (sandwich elisa) has been ...201627312522
a prospective view of animal and human fasciolosis.fasciolosis, a food-borne trematodiasis, results following infection with the parasites, fasciola hepatica and fasciola gigantica. these trematodes greatly affect the global agricultural community, infecting millions of ruminants worldwide and causing annual economic losses in excess of us $3 billion. fasciolosis, an important zoonosis, is classified by who as a neglected tropical disease with an estimated 17 million people infected and a further 180 million people at risk of infection. the sign ...201627314903
cytosolic superoxide dismutase can provide protection against fasciola gigantica.superoxide dismutases (sod), antioxidant metallo-enzymes, are a part of the first line of defense in the trematode parasites which act as the chief scavengers for reactive oxygen species (ros). a recombinant fasciola gigantica cytosolic sod (fgsod) was expressed in escherichia coli bl21 (de3) and used for immunizing rabbits to obtain polyclonal antibodies (anti-rfgsod). this rabbit anti-rfgsod reacted with the native fgsod at a molecular weight of 17.5kda. the fgsod protein was expressed at high ...201627338185
a retrospective study of the prevalence of bovine fasciolosis at major abattoirs in botswana.a retrospective study covering a period of ten years (2001-2010) was conducted using postmortem meat inspection records of the department of veterinary services in gaborone to determine the prevalence of bovine fasciolosis in botswana. meat inspection records of monthly and annual returns from the two main export abattoirs in the country were examined, as well as the data collected on the total number of cattle slaughtered and the number of livers condemned due to fasciola gigantica infection. o ...201627380655
identification of chalcones as fasciola hepatica cathepsin l inhibitors using a comprehensive experimental and computational approach.increased reports of human infections have led fasciolosis, a widespread disease of cattle and sheep caused by the liver flukes fasciola hepatica and fasciola gigantica, to be considered an emerging zoonotic disease. chemotherapy is the main control measure available, and triclabendazole is the preferred drug since is effective against both juvenile and mature parasites. however, resistance to triclabendazole has been reported in several countries urging the search of new chemical entities and t ...201627463369
vaccine potential of recombinant cathepsinl1g against fasciola gigantica in this study, we characterized and investigated the vaccine potential of fgcatl1g against fasciola gigantica infection in mice. recombinant mature fgcatl1g (rmfgcatl1g) was expressed in escherichia coli bl21. the vaccination was performed in imprinting control region (icr) mice (n=10) by subcutaneous injection with 50μg of rmfgcatl1g combined with freund's adjuvant. two weeks after the second boost, mice were infected with 15 metacercariae by the oral route. the percents of protection of rmfgca ...201627514897
cross-sectional study of fasciola gigantica and other trematode infections of cattle in edu local government area, kwara state, north-central nigeria.trematode infections of livestock are of global veterinary and public health importance causing serious economic losses. majority of data on burden of trematode infections in nigeria are based on abattoir surveys and there are very few data on herd level risk factors. the present study investigated the prevalence of, and herd level risk factors for, fasciolosis and other trematode infections in cattle in edu local government area (lga).201627565768
effect of induced fasciola gigantica infection during pre-patency on the performance of buffalo calves fed on different percentage of protein.thirty growing murrah buffalo calves (8-12 months of age, 109.85 ± 2.43) were reared in parasite free conditions and randomly divided into three equal groups as per crd. they were fed on iso-caloric (2.01 me mcal/kg diet) diets containing standard protein (sp) diet at 100 %, 90 % of sp (medium protein, mp) and 80 % of sp (low protein, lp) of the protein requirements (kearl 1982). after 21 days of feeding, each group was further subdivided into two sub-groups (a & b). animals in sub-groups 'a' se ...201627605773
2d-page analysis of the soluble proteins of the tropical liver fluke, fasciola gigantica and biliary amphistome, gigantocotyle explanatum, concurrently infecting bubalus bubalis.the digenetic trematodes, fasciola gigantica and gigantocotyle explanatum, belonging to the family fasciolidae and paramphistomidae respectively, have been often found to concurrently infect the liver of indian water buffalo bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. in the present study the soluble gene products of both f. gigantica and g. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. the soluble prote ...201627605808
development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis.cystic echinococcosis (ce) or hydatidosis, caused by the larval stage of echinococcus granulosus (eg)-complex, is a neglected parasitic disease of public health importance. the disease is endemic in many african and mediterranean countries including the sudan. the objective of the present study was to develop and evaluate a real-time loop-mediated isothermal amplification (lamp) assay for simple and rapid detection of ce in humans and domestic live stock in sudan.201627619674
toxicity of chlorophyllin against lymnaea acuminata at different wavelengths of visible light.fasciolosis is a water and food-borne disease caused by the liver fluke fasciola hepatica and fasciola gigantica. this disease is widespread in different parts of the world. lymnaeidae and planorbidae snails are the intermediate hosts of these flukes. snail population management is a good tool to control fasciolosis because gastropods represent the weakest link in the life-cycle of trematodes. chlorophyll can be extracted from any green plant. chlorophyllin was prepared from spinach in 100% etha ...201627688849
molecular characterization and phylogenetic analysis of fasciola gigantica from nigeria.fasciola gigantica is considered the major pathogen causing fasciolosis in africa; however, molecular characterization of this fluke has not been adequately elucidated. it is important to scientifically elucidate the dispersal history of f. gigantica by analyzing its genetic diversity. fasciola flukes from nigeria were analyzed using nuclear and mitochondrial dna markers. a total of 172 fasciola flukes collected from cattle were identified as f. gigantica because they displayed the f. gigantica ...201727771460
molecular and immunological characterisation of fasciola species.fasciola hepatica and f. gigantica are polymorphic liver flukes that show considerable overlap between species, and various protein separation techniques have been used as alternative means of differentiation. acid and alkaline polyacrylamide gel electrophoresis (page) show differences between f. hepatica and f. gigantica. following sds-page, f. hepatica proteins are characterised by the presence of eight major peptide bands, with molecular weights estimated as 48, 45, 43.5, 37, 33, 29, 27 and 2 ...200227786093
nuclear and mitochondrial dna analysis reveals that hybridization between fasciola hepatica and fasciola gigantica occurred in china.the well-known pathogens of fasciolosis, fasciola hepatica (fh) and fasciola gigantica (fg), possess abundant mature sperms in their seminal vesicles, and thus, they reproduce bisexually. on the other hand, aspermic fasciola flukes reported from asian countries, which have no sperm in their seminal vesicles, probably reproduce parthenogenetically. the aim of this study was to reveal the origin of aspermic fasciola flukes. the nuclear single copy markers, phosphoenolpyruvate carboxykinase and dna ...201727804894
comparison between in-house indirect elisa and dot-elisa for the diagnosis of fasciola gigantica in cattle.this survey was done to investigate the efficacy of the in-house indirect elisa (ielisa) and dot-elisa methods prepared from excretion-secretory (es ag) and crude (cr ag) antigens of fasciola for sero-diagnosis of fasciola gigantica in cattle. the liver specimens of slaughtered cattle were collected and their liver examined macroscopically and microscopically for infestation to fasciolosis. sera from two groups of cattle, one infected with fasciolosis (n = 60) and the other non-infected with fas ...201627876956
detection of fasciola hepatica and fasciola gigantica common and uncommon antigens, using rabbit hyper immune serum raised against their excretory-secretory and somatic antigens.fasciolosis is an important neglected helminth disease caused by two liver flukes, fasciola hepatica and fasciola gigantica. the two species of fasciola are usually different in their morphological and molecular features. they have also common and uncommon antigens in both their somatic and excretory secretory metabolites. in this study, we compared somatic and excretory-secretory (es) antigens of f. hepatica and f. gigantica, by using rabbit hyper immune serum raised against these antigens. adu ...201627876981
re-infection with fasciola gigantica 6-month post-treatment with triclabendazole in cattle from mobile pastoralist husbandry systems at lake lake chad in central africa, livestock fascioliasis caused by fasciola gigantica represents a major veterinary health problem, particularly in cattle reared in mobile pastoralist husbandry systems. we assessed re-infection after a single dose of triclabendazole with fascioliasis in cattle in a mobile pastoralist setting towards the end of the dry season. within the cattle herds of 14 groups of mobile pastoralists, 375 cattle were randomly selected. a faecal sample was obtained from each anima ...201627884440
animal fascioliasis: perspectives from high altitudinal regions.the parasitic flukes of the genus fasciola (platyhelminthes: trematoda: digenea) cause fascioliasis or liver-rot disease in ruminant livestock in tropical and sub-tropical regions of the world. classically, two species of fasciola- f. hepatica and f. gigantica, are universally recognized as taxonomically valid species. our survey studies on ovid and bovid animals including yak and mithun from high altitudinal mountainous regions in northeast india revealed the occurrence of fasciola gigantica an ...201627890078
identity of fasciola spp. in sheep in egypt, liver flukes, fasciola spp. (digenea: fasciolidae), have a serious impact on the farming industry and public health. both fasciola hepatica and fasciola gigantica are known to occur in cattle, providing the opportunity for genetic recombination. little is known on the identity and genetic variability of fasciola populations in sheep.201627906040
molecular characterization of animal fasciola spp. isolates from kermanshah, western iran.we evaluated the genetic diversity of samples identified morphologically as fasciola spp. from sheep, cattle and goat from kermanshah province, western iran using pcr-rflp method.201627957438
characterization and vaccine potential of fasciola gigantica saposin-like protein 1 (sap-1).the recombinant fasciola gigantica saposin-like protien-1 (rfgsap-1) was cloned by polymerase chain reaction (pcr) from nej cdna, expressed in escherichia coli bl21 (de3) and used for production of a polyclonal antibody in rabbits (anti-rfgsap-1). by immunoblotting and immunohistochemistry, rabbit igg anti-rfgsap-1 reacted with rfgsap-1 at a molecular weight 12kda, but not with rfgsap-2. the rfgsap-1 reacted with antisera from mouse infected with f. gigantica metacercariae collected at 2, 4, and ...201728043381
photomediated larvicidal activity of pheophorbide a against cercaria larvae of fasciola gigantica.fasciolosis is a parasitic disease caused by fasciola gigantica. the freshwater snail lymnaea acuminata is the intermediate host of f. gigantica which cause endemic fasciolosis in the northern part of india. to investigate larvicidal activity of pure and laboratory extracted pheophorbide a (pa) against cercaria larvae of f. gigantica, data were analyzed in different spectra of visible light, sunlight, and laboratory conditions. photostimulation of chlorophyll derivative pheophorbide a (pa) cause ...201728127494
transcriptomic responses of water buffalo liver to infection with the digenetic fluke fasciola gigantica.fasciola gigantica, the tropical liver fluke, infects buffaloes in asian and african countries and causes significant economic losses and poses public health threat in these countries. however, little is known of the transcriptional response of buffaloes to infection with f. gigantica. the objective of the present study was to perform the first transcriptomic analysis of buffalo liver infected by f. gigantica. understanding the mechanisms that underpin f. gigantica infection in buffaloes will co ...201728143561
anthelmintic potential of thymoquinone and curcumin on fasciola gigantica.fasciolosis an economically important global disease of ruminants in the temperate and tropical regions, caused by fasciola hepatica and f. gigantica, respectively, also poses a potential zoonotic threat. in india alone it causes huge losses to stakeholders. anthelmintics including triclabendazole have been used to control this menace but the emerging resistance against the available compounds necessitates identification of novel and alternative therapeutic measures involving plant derived natur ...201728152102
fasciola hepatica infections in cattle and the freshwater snail galba truncatula from dakhla oasis, egypt.infection by fasciola species was investigated in seven districts of dakhla oasis, egypt, through abattoir inspection of cattle livers for adult worms and sedimentation of faecal samples from local cattle to detect fasciola eggs. in addition, lymnaeid snails collected from the study area were examined microscopically for developmental stages of fasciola spp. abattoir inspection revealed that 51 out of 458 cattle livers (11.1%) contained adult flukes, which were identified morphologically as fasc ...201728162101
immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich elisa for detection of paramphistomum gracile circulating 16 kda this study, we have produced a monoclonal antibody (moab) against 16 kda antigen of paramphistomum gracile (16 kdaagpg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich elisa) for the detection of circulating 16 kdaag in the serum and fecal samples from cattle naturally infected with p. gracile. moab 1d10 was immobilized on a microtitre plate, and the antigen in the samples was captured and detected with biotinylated rabbit anti-16 kdaagpg antibody. the lower de ...201728219472
fasciolopsis buski (digenea: fasciolidae) from china and india may represent distinct taxa based on mitochondrial and nuclear ribosomal dna sequences.fasciolopsis buski is a zoonotic intestinal fluke infecting humans and pigs, but it has been seriously neglected. it is yet to know whether there is any genetic diversity among f. buski from different geographical locations, particularly in sequences of nuclear ribosomal dna (rdna) and mitochondrial (mt) dna. therefore, we determined the sequences of partial 18s, the complete internal transcribed spacer (its) rdna and the complete mt genome of f. buski from china, compared the rdna and mtdna seq ...201728228149
de novo transcriptome sequencing and analysis of the juvenile and adult stages of fasciola gigantica.fasciola gigantica is regarded as the major liver fluke causing fasciolosis in livestock in tropical countries. despite the significant economic and public health impacts of f. gigantica there are few studies on the pathogenesis of this parasite and our understanding is further limited by the lack of genome and transcriptome information. in this study, de novo illumina rna sequencing (rna-seq) was performed to obtain a comprehensive transcriptome profile of the juvenile (42days post infection) a ...201728286139
chronic bovine fasciolosis associated cholangiolithiasis: a case report.cholangiolithiasis is a rare condition in animals wherein the choleliths are formed in the biliary tracts causing chronic inflammation. in the current study, choleliths which were firm and friable concretions of approximately 3-4 cm in diameter were found in the bile ducts of the liver of an adult cattle infected with fasciola gigantica. two such irregularly shaped concretions were encountered in the two biliary tracts leading to common bile duct in the liver. there were localized irregular sacc ...201728316393
fasciola hepatica immune regulates cd11c(+) cells by interacting with the macrophage gal/galnac lectin.fasciolosis, caused by fasciola hepatica and fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. like other helminths, f. hepatica modulates the host immune response by inducing potent polarized th2 and regulatory t cell immune responses and by downregulating the production of th1 cytokines. in this work, we show that f. hepatica glycans increase th2 immune responses by immunomodulating tlr-induced maturation and function of dendritic cells (dcs). t ...201728360908
prevalence, economic analysis and chemotherapeutic control of small ruminant fasciolosis in the sargodha district of punjab, pakistan.this study describes the epidemiology, the economic significance of small ruminant fasciolosis in animals slaughtered in the abattoirs of the sargodha district, punjab, pakistan between january and june 2012. in vivo fasciolicidal efficacy of commercially available compounds was examined using a randomised complete block design. microscopically screened faecal samples revealed 40.51% positive animals for fasciolosis. the prevalent species included fasciola hepatica (35.64%) and fasciola gigantic ...201728365925
molecular and morphometrical characterization of fasciola species isolated from domestic ruminants in ardabil province, northwestern iran.we aimed to describe morphological and morphometrical characteristics of fasciola spp. in livestock from ardabil province, northwest iran.201728435817
a novel ex vivo immunoproteomic approach characterising fasciola hepatica tegumental antigens identified using immune antibody from resistant sheep.a more thorough understanding of the immunological interactions between fasciola spp. and their hosts is required if we are to develop new immunotherapies to control fasciolosis. deeper knowledge of the antigens that are the target of the acquired immune responses of definitive hosts against both fasciola hepatica and fasciola gigantica will potentially identify candidate vaccine antigens. indonesian thin tail sheep express a high level of acquired immunity to infection by f. gigantica within 4w ...201728455238
a review of bovine fasciolosis and other trematode infections in nigeria.trematode infections cause serious economic losses to livestock worldwide. global production losses due to fasciolosis alone exceed us$3 billion annually. many trematode infections are also zoonotic and thus a public health concern. the world health organization has estimated that about 56 million people worldwide are infected by at least one zoonotic trematode species, and up to 750 million people are at risk of infection. fasciolosis caused by the fluke fasciola gigantica is endemic in nigeria ...201728528590
gastrointestinal helminthosis: prevalence and associated determinants in goats of jabalpur, india.a total of 632 faecal samples of goats of jabalpur district of madhya pradesh state were examined during three years of the study period (november 2012-october 2015), out of which 82.75 % samples were positive for helminthic infections. among various helminths, maximum prevalence was of strongyles (32.59 %) followed by amphistomes (14.40 %), moniezia spp. (12.50 %), strongyloides spp. (0.79 %), trichuris spp. (0.47 %) and fasciolagigantica (0.32 %). year wise prevalence revealed a highly signifi ...201728615851
genotoxic potential of fasciolagigantica infection in experimentally infected rabbits.a number of parasitic platyhelminthes are known to cause genotoxicity in humans and animals. however no such information is available on tropical liver fluke, fasciola gigantica, which incurs huge economic losses worldwide. in the present study the genotoxic potential of f. gigantica infection in rabbits, experimentally infected with the metacercarial cysts of this parasite, has been investigated using the standard comet assay and micronucleus (mni) test on the isolated hepatocytes and the whole ...201728615853
identification of potential inhibitors of fasciola gigantica thioredoxin1: computational screening, molecular dynamics simulation, and binding free energy studies.fasciola gigantica is the causative organism of fascioliasis and is responsible for major economic losses in livestock production globally. f. gigantica thioredoxin1 (fgtrx1) is an important redox-active enzyme involved in maintaining the redox homeostasis in the cell. to identify a potential anti-fasciolid compound, we conducted a structure-based virtual screening of natural compounds from the zinc database (n = 1,67,740) against the fgtrx1 structure. the ligands were docked against fgtrx1 and ...201728627969
immunodiagnosis of human fascioliasis: an update of concepts and performances of the serological assays.human fascioliasis (hf) is a foodborne neglected parasitic disease caused by fasciola hepatica and fasciola gigantica. new epidemiological data suggest that the endemic areas of the disease are expanding and hf is being reported from areas where it was previously not observed. diagnosis of hf is challenging. performances of parasitological approaches, based on the detection of parasite's egg in the stool, are not satisfactory. currently serological methods for the diagnosis of hf are mainly base ...201728764235
the in vitro anthelmintic activity of the ethanol leaf extracts of terminalia catappa l. on fasciola present, there are no medicinal plant extracts currently available for treatment and control of fasciolosis. the present work could provide, for the first study, conclusions on the in vitro fasciolicidal properties of the ethanol extract of terminalia catappa l. (tcce) leaves against adult fasciola gigantica after incubation with rpmi-1640 medium containing the tcce at various concentrations and times when compared with triclabendazole (tcz). the relative motility and survival index values of ...201728805167
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