PMID(sorted ascending)
tegumental histological effects of mirazid(®) and myrrh volatile oil on adult fasciola evaluated the histological changes within the tegument of adult fasciola gigantica (f. gigantica) that led to the gross changes that were visible externally.201323730566
phenotypes of intermediate forms of fasciola hepatica and f. gigantica in buffaloes from central punjab, pakistan.fascioliasis is an important food-borne parasitic disease caused by the two trematode species, fasciola hepatica and fasciola gigantica. the phenotypic features of fasciolid adults and eggs infecting buffaloes inhabiting the central punjab area, pakistan, have been studied to characterize fasciolid populations involved. morphometric analyses were made with a computer image analysis system (cias) applied on the basis of standardized measurements. since it is the first study of this kind undertake ...201423731975
vaccine potential of recombinant cathepsin b against fasciola fasciola gigantica, cathepsin bs, especially cathepsin b2 and b3 are expressed in early juvenile stages, and are proposed to mediate the invasion of host tissues. thus they are thought to be the target vaccine candidates that can block the invasion and migration of the juvenile parasite. to evaluate their vaccine potential, the recombinant cathepsin b2 (rfgcatb2) and cathepsin b3 (rfgcatb3) were expressed in yeast, pichia pastoris, and used to immunize mice in combination with freund's adjuva ...201323811052
molecular cloning, characterization and functional analysis of a novel juvenile-specific cathepsin l of fasciola gigantica.cathepsin l proteases are a major class of endopeptidases expressed at a high level in fasciola parasites. several isoforms of cathepsin l were detected and they may perform different functions during the parasite development. in this study, a complete cdna encoding a cathepsin l protease was cloned from a newly excysted juvenile (nej) cdna library of fasciola gigantica and named fgcatl1h. it encoded a 326 amino acid preproenzyme which shared 62.8-83.1% and 39.5-42.9% identity to fasciola spp. a ...201323820262
direct and indirect affection of the central nervous system by fasciola infection.fascioliasis is a worldwide, zoonotic disease caused by the liver trematodes fasciola hepatica and fasciola gigantica. neurological fascioliasis has been widely reported in all continents, affecting both sexes and all ages. two types of records related to two physiopathogenic mechanisms may be distinguished: cases in which the neurological symptoms are due to direct effects of a migrating juvenile present in the brain or neighboring organ and with cerebral lesions suggesting migration through th ...201323829920
possible fasciocidal activity of methanol extract of dregea volubilis eastern and southern part of india dregea volubilis (family asclepediaceae) is widely used as anthelmintic in traditional system of medicine. the present study was conducted to evaluate the fasciocidal activity of the methanol extract of d. volubilis leaves (medv) and to observe the drug's effect on organisms through scanning electron microscopic (sem) study. live parasites (trematode: fasciola gigantica) were collected in 0.9% phosphate-buffered saline from the bile ducts of buffalo. those w ...201323850996
th1 and th2 cytokine gene expression in primary infection and vaccination against fasciola gigantica in buffaloes by real-time pcr.th1 and th2 cytokine gene expression in buffalo calves during primary infection with fasciola gigantica as well as in response to immunization with the parasite recombinant fatty acid binding protein (rfabp) and recombinant glutathione s-transferase (rgst) proteins was measured at 14th week of infection by real-time pcr with the double-stranded dna-binding dye sybr green. experimental animals were randomly distributed into fabp, gst, cocktail, challenge and healthy groups. animals in groups fabp ...201323873619
[effect evaluation of three elisa kits in detection of fasciolasis].to evaluate the effect of 3 elisa kits on detection of human fasciolasis.201323894840
immunization with recombinant leucine aminopeptidase showed protection against fasciola gigantica in mice.leucine aminopeptidase (lap) is expressed in all stages of fasciola gigantica and, hence, is considered as a potential vaccine candidate. in this study, we have tested a vaccine potential of lap and the types of immune responses it elicited in vaccinated mice. recombinant f. gigantica leucine aminopeptidase (rfglap) was expressed in escherichia coli, bl21 (de3). the imprinting control region mice subcutaneously immunized with 50 μg of rfglap combined with freund's adjuvant (n = 10) exhibited a s ...201323917327
immunodetection of fasciola gigantica circulating antigen in sera of infected individuals for laboratory diagnosis of human fascioliasis.currently, the laboratory diagnosis of human fascioliasis is based on the parasitological examination of parasite eggs in stool specimens and serological detection of specific antibodies in serum samples, which are often unreliable diagnostic approaches. ideally, a sensitive and specific diagnostic test for fasciola infection should be based on the detection of circulating fasciola antigen, which implies active infection. here, a 27-kda-molecular-mass antigen was identified in a fasciola giganti ...201323945158
an outbreak of human fascioliasis gigantica in southwest china.fascioliasis is a common parasitic disease in livestock in china. however, human fascioliasis is rarely reported in the country. here we describe an outbreak of human fascioliasis in yunnan province. we reviewed the complete clinical records of 29 patients and performed an epidemiological investigation on the general human population and animals in the outbreak locality. our findings support an outbreak due to fasciola gigantica with a peak in late november, 2011. the most common symptoms were r ...201323951181
development and hatching mechanism of fasciola eggs, light and scanning electron microscopic studies.both light microscopy and scanning electron microscopy were used in the description. there were variable measurements of eggs from the same fluke and there was no relationship between the size of the fluke and size of eggs. light microscopy revealed that the operculum has different shapes in fasciola gigantica and f. hepatica. under normal laboratory conditions of temperature (26 ± 1 °c), miracidia of f. gigantica developed within 12-16 days period, but those of f. hepatica developed within a pe ...201023961086
immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of fasciola gigantica paramyosin antigen.many immunological techniques have been developed over years using the different fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques, which are time consuming and usually lack sensitivity and reproducibility.201323961441
vaccine potential of recombinant saposin-like protein 2 against fasciolosis gigantica in mice.saposin-like protein 2 (sap-2) is a protein that adult of fasciola spp. use to lyse plasma membrane of red blood cells, so that their contents can be digested by proteases for the parasites' nutrients. thus sap-2 is a plausible target for vaccination against these parasites. recombinant fasciola gigantica saposin-like protein 2 (rfgsap-2) was expressed in escherichia coli bl21 (de3). a vaccination was performed in icr mice (n=10) by subcutaneous injection with 50μg of rfgsap-2 combined with freu ...201324075921
comparative toxicity of paraquat herbicide and some plant extracts in lymnaea natalensis snails.paraquat has been shown to be a highly toxic compound for humans and animals, and many cases of acute poisoning and death have been reported over the past few decades. the present study was undertaken to evaluate comprehensively herbicides (paraquat) and some plant extracts to biochemical aspects of lymnaea natalensis snails. it was found that the exposure of l. natalensis to paraquat and plant extracts led to a significant reduction in the infectivity of fasciola gigantica miracidia to the snai ...201624081640
immunodiagnosis as an aid for early detection of fasciola gigantica by glutathione s-transferase (gst).in the present study, effort to isolate purified gst from fasciola gigantica homogenate and its early detection for confirmation of infection has been successful. in this study, immunoprotection studies with gst and antibody titer post-infection has revealed gst to be an immunogenic protein with high sensitivity to detect anti-fasciola antibody as early as two weeks post-infection in large ruminants.201224082529
the in vitro anthelmintic effects of plumbagin on newly excysted and 4-weeks-old juvenile parasites of fasciola gigantica.the effect of plumbagin (pb, 5-hydroxy-2-methyl-1,4-naphthoquinone) against newly excysted juveniles (nejs) and 4-weeks-old immature parasites of fasciola gigantica were compared with triclabendazole (tcz). the anthelmintic efficacy of 1, 10 and 100μg/ml of pb or tcz following incubation in vitro for 1-24h was compared using a combination of relative motility (rm), survival index (si) and larval migration inhibition (lmi) assays for parasite viability. the rm and si values of the pb-treated grou ...201424157317
tertiary combination of freeze-dried urine of indian breeds of cow with plant products against snail lymnaea acuminata.snail lymnaea acuminata is the intermediate host of liver fluke fasciola gigantica, which cause endemic fasciolosis among cattle population of eastern uttar pradesh. control of snail population by molluscicides is one of the effective methods to control fasciolosis. in the present study molluscicidal activity of tertiary combination of freeze-dried urine of different indian breeds of cow sahiwal, geer and tharparkar with annona squamosa seed powder, ferula asafoetida root latex, azadirachta indi ...201224199478
praziquantel treatment in trematode and cestode infections: an update.status and emerging issues in the use of praziquantel for treatment of human trematode and cestode infections are briefly reviewed. since praziquantel was first introduced as a broadspectrum anthelmintic in 1975, innumerable articles describing its successful use in the treatment of the majority of human-infecting trematodes and cestodes have been published. the target trematode and cestode diseases include schistosomiasis, clonorchiasis and opisthorchiasis, paragonimiasis, heterophyidiasis, ech ...201324265948
neurological and ocular fascioliasis in humans.fascioliasis is a food-borne parasitic disease caused by the trematode species fasciola hepatica, distributed worldwide, and fasciola gigantica, restricted to given regions of africa and asia. this disease in humans shows an increasing importance, which relies on its recent widespread emergence related to climate and global changes and also on its pathogenicity in the invasive, biliary, and advanced chronic phases in the human endemic areas, mainly of developing countries. in spite of the large ...201424480313
serodiagnosis of bovine fasciolosis by dot-enzyme immuno assay in chennai, tamil nadu.excretory/secretory (e/s) antigen of adult fasciola gigantica was used for diagnosis of fasciolosis in cattle and buffaloes sera from slaughter houses and different places in and around chennai, tamil nadu. a total of 6.4 mg of e/s antigen was obtained from the 60 flukes collected from slaughter houses. coprological examination was done to detect endoparasitic infection in 321 fecal samples. dot-enzyme immuno assay (dot-eia) was standardized with optimum working dilution of e/s antigen, serum, a ...201424505174
survey on gastrointestinal parasites and detection of cryptosporidium spp. on cattle in west java, evaluate the presence of gastrointestinal parasites on cattle in indonesia because the prevalence of parasites varies between countries depending on the terrain surrounding livestock farms and investigations in indonesia have never been performed.201424507639
prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region.201424507641
protection against fasciola gigantica using paramyosin antigen as a candidate for vaccine production.yet no vaccine to protect ruminants against liver fluke infection has been commercialized. in an attempt to develop a suitable vaccine against fasciola gigantica (f. gigantica) infection in rabbits, using 97 kda pmy antigen. it was found that, the mean worm burdens and bile egg count after challenge were reduced significantly by 58.40 and 61.40%, respectively. on the other hand, immunization of rabbits with pmy induced a significant expression of humoral antibodies (igm, total igg, igg1, igg2 an ...201324511686
invasion biology meets parasitology: a case study of parasite spill-back with egyptian fasciola gigantica in the invasive snail pseudosuccinea columella.the liver fluke fasciola gigantica is a trematode parasite of ruminants and humans that occurs naturally in africa and asia. cases of human fascioliasis, attributable at least in part to f. gigantica, are significantly increasing in the last decades. the introduced snail species galba truncatula was already identified to be an important intermediate host for this parasite and the efficient invader pseudosuccinea columella is another suspect in this case. therefore, we investigated snails collect ...201424523913
a consistent pcr-rflp assay based on its-2 ribosomal dna for differentiation of fasciola species.fascioliasis is a zoonotic parasitic disease caused by liver fluke species of fasciola hepatica and fasciola gigantica. differentiation of these two species, based on their morphological characteristics, is difficult. the current study aimed to use pcr-rflp assay to distinguish between f. hepatica and f. gigantica, based on profiles of rflp, produced by effect of endonucleases on its2 of the ribosomal dna genes from these two species.201324570833
analysis of the expression and antioxidant activity of 2-cys peroxiredoxin protein in fasciola gigantica.2-cys peroxiredoxin (prx) is the main antioxidant enzyme in fasciola species for detoxifying hydrogen peroxide which is generated from the hosts' immune effector cells and the parasites' own metabolism. in this study, the recombinant prx protein from fasciola gigantica (rfgprx-2) was expressed and purified in a prokaryotic expression system. this recombinant protein with molecular weight of 26 kda was enzymatically active in reduction of hydrogen peroxide both in presence of thioredoxin and glut ...201424594261
role of oxidative stress in concomitant occurrence of fasciola gigantica and leiomyoma in cattle.fasciola gigantica is a parasitic helminth that predominantly infects the liver and bile ducts of cattle and causes great losses of cattle production in the southwestern regions of iran. the purpose of the present study was to find out the possible relationship between the extent of liver destruction and oxidative stress by estimating the level of mda, and sod and gpx enzymes in the liver of cattle infected with f. gigantica. studies were carried out on 49 infected and 20 healthy livers. based o ...201424636711
immunodiagnosis of egyptian human fasciolosis gigantica using fas1 and fas2 cysteine proteinase antigens.fasciolosis caused by fasciola gigantica is one of the major public health problems in the world including egypt. immunodiagnostic methods are more applicable for their better sensitivity and specificity than other methods. the present study was conducted to cysteine proteinase (cp) antigens of f. gigantica in igg-elisa to diagnose human fasciolosis. igg-elisa with 2 cysteine proteinases of 27 kda (fas1) and 29 kda (fas2), obtained from the regurgitated materials of adult worms, were evaluated u ...201324640878
trematode infections in cattle in arumeru district, tanzania are associated with irrigation.the relationship between the environment and infection of cattle with trematodes was studied at arumeru district, arusha region, northern tanzania. randomly selected villages were grouped into three cattle management strata, (i) zero-grazing (zz) (ii) communal grazing without irrigation (zc) and (iii) communal grazing with irrigation (zci).201424650420
complete mitochondrial genomes of the 'intermediate form' of fasciola and fasciola gigantica, and their comparison with f. hepatica.fascioliasis is an important and neglected disease of humans and other mammals, caused by trematodes of the genus fasciola. fasciola hepatica and f. gigantica are valid species that infect humans and animals, but the specific status of fasciola sp. ('intermediate form') is unclear.201424685294
prevalence of fasciola gigantica infection in slaughtered animals in south-eastern lake chad area in relation to husbandry practices and seasonal water levels.fasciolosis has been described in sub-saharan africa in many accounts, but the latest reports from chad are from the 1970s. mobile pastoralists perceive liver parasites as a significant problem and think that proximity to lake chad can lead to infection. this study aimed to assess the importance of liver fluke infections in mobile pastoralists' livestock in the south-eastern lake chad 2011, all animals presented at three slaughter slabs near gredaya in the south-eastern lake chad area ...201424708774
identification and expression of fasciola gigantica the present study, a cdna encoding trx from f. gigantica (fgtrx) was cloned by polymerase chain reaction (pcr). the sequence of fgtrx, analyzed by blast, signalp, and clustralw programs, showed 315 bp of an open reading frame (orf), 12 bp 5'utr, 78 bp 3'utr, and the putative fgtrx peptide comprising of 104 amino acids, with a molecular weight of 11.68 kda, with the active site containing five amino acids (tryptophan, cysteine, glycine, proline, cysteine) with a conserved dithiol motif from th ...201424718754
production and characterization of a monoclonal antibody against recombinant cathepsin l1 of fasciola gigantica.monoclonal antibodies (moabs) against a recombinant cathepsin l1 of fasciola gigantica (rfgcatl1) were produced in vitro by fusion of balb/c mice spleen cells immunized with rfgcatl1 and mouse myeloma cells. reactivity and specificity of these moabs were evaluated by indirect elisa and immunoblotting techniques. seven moab clones were selected from the stable hybridoma clones, namely 1e10, 1f5, 3d11, 4b10, 4d3, 4e3 and 5e7. clones 1e10, 1f5 and 3d11 were igm, whereas clones 4b10, 4d3, 4e3 and 5e ...201424736227
time-dependent tegumental surface changes in juvenile fasciola gigantica in response to triclabendazole treatment in goat.triclabendazole (tcbz), the anthelmintic drug active against both mature and immature liver flukes, was used to investigate the effect of in vivo treatment on the tegumental surface of juvenile fasciola gigantica. five goats were infected with 150 f. gigantica metacercariae each by oral gavage. four of them were treated with single dose of tcbz at 10mg/kg at four weeks post-infection. they were euthanized at 0 (untreated), 24, 48, 72 and 96h post treatment. juvenile flukes were manually retrieve ...201424742909
characteristics and molecular phylogeny of fasciola flukes from bangladesh, determined based on spermatogenesis and nuclear and mitochondrial dna analyses.this study aimed to precisely discriminate fasciola spp. based on dna sequences of nuclear internal transcribed spacer 1 (its1) and mitochondrial nicotinamide adenine dinucleotide (nadh) dehydrogenase subunit 1 (nad1) gene. we collected 150 adult flukes from the bile ducts of cattle, buffaloes, sheep, and goats from six different regions of bangladesh. spermatogenic status was determined by analyzing stained seminal vesicles. the its1 types were analyzed using the polymerase chain reaction-restr ...201424781019
[comparison between albendazole and triclabendazole against fasciola gigantica in human].to compare the anthelmintic effect of albendazole with that of triclabendazole against fasciola gigantica.201424800585
thiosulphate: cyanide sulphur transferase activity in some species of helminth parasites.investigation on the characteristic pattern of enzymes in various organisms may be of particular importance, as this type of information can help to understand certain physiological and biochemical processes in living organisms. thiosulfate: cyanide sulfurtransferase (rhodanese) is a ubiquitous multifunctional enzyme, which its major function is generally believed to be in cyanide detoxification. the present study was conducted to determine the activity of this enzyme in some parasitic helminths ...201424808648
[case report: fasciola gigantica infection treated by triclabendazole]. 201324809182
a case of fasciola hepatica infection mimicking cholangiocarcinoma and its-1 sequencing of the worm.fascioliasis is a zoonotic infection caused by fasciola hepatica or fasciola gigantica. we report an 87-year-old korean male patient with postprandial abdominal pain and discomfort due to f. hepatica infection who was diagnosed and managed by endoscopic retrograde cholangiopancreatography (ercp) with extraction of 2 worms. at his first visit to the hospital, a gallbladder stone was suspected. ct and magnetic retrograde cholangiopancreatography (mrcp) showed an intraductal mass in the common bile ...201424850964
first insight into cd59-like molecules of adult fasciola hepatica.the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ...201424955521
molecular and morphological characterization of fasciola spp. isolated from different host species in a newly emerging focus of human fascioliasis in iran.the current study aimed to find out the morphometric and genotypic divergences of the flukes isolated from different hosts in a newly emerging focus of human fascioliasis in iran. adult fasciola spp. were collected from 34 cattle, 13 sheep, and 11 goats from kohgiluyeh and boyer-ahmad province, southwest of iran. genomic dna was extracted from the flukes and pcr-rflp was used to characterize the isolates. the its1, its2, and mitochondrial genes (mtdna) of ndi and coi from individual liver flukes ...201425018891
molecular phylogenetic identification of fasciola flukes in nepal.eighty-one fasciola flukes collected from 8 districts in nepal were analyzed for their species identification on the basis of their spermatogenic status and nuclear ribosomal internal transcribed spacer 1 (its1) and for their phylogenetic relation with fasciola flukes from other asian countries on the basis of the mitochondrial nadh dehydrogenase subunit 1 (nad1) gene. sixty-one flukes (75.3%) were aspermic fasciola sp., and 20 flukes (24.7%) were identified as fasciola gigantica. all of the asp ...201425025757
effect of feeding deoiled mahua (bassia latifolia) seed cake on the growth performance, digestibility and balance of nutrients in cross-bred calves during pre-patent period of fasciola gigantica infection.a study was conducted to assess the growth performance and nutrient utilization of cross-bred calves serially infected with fasciola gigantica and/or supplemented with deoiled mahua seed cake (dmsc) during pre-patent period of infection. twenty healthy cross-bred (holstein friesian × haryana) male calves aged 6-7 months with an initial average live weight of 79.6 ± 2.71 kg were divided into four treatment groups (con, con-inf, dmc and dmc-inf) of five animals each following randomized block desi ...201525065976
fasciola gigantica transmission in the zoonotic fascioliasis endemic lowlands of guilan, iran: experimental assessment.the lowland flatlands around the city of bandar-anzali, at the caspian sea shore, guilan province, are an endemic area where fasciola gigantica appears to be the fasciolid species involved and past outbreaks affecting around 15,000 people and the highest human infection rates in iran have been reported. fascioliasis transmission in that area has been experimentally analysed for the first time, by means of assays of monomiracidial (group a: 120 snails) and pentamiracidial (group b: 96 snails) inf ...201425095734
opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like.concurrent deficiency of rhophilin associated tail protein (ropn1) and ropn1-like (ropn1l) in mice causes structural abnormalities and immotility of sperm and thereby infertility. in the present research, ropn1l of the human liver fluke opisthorchis viverrini was molecularly characterized and showed unexpected potential as a diagnostic tool. ropn1l transcripts were detected in 2-week-old juveniles by rt-pcr. immunohistochemical analysis of the adult worm localized the protein in testis lobes, se ...201425124046
a 170kda multi-domain cystatin of fasciola gigantica is active in the male reproductive system.cystatins are functional as intra- and extracellular inhibitors of cysteine proteases and are expressed as single or multi-domain proteins. we have previously described two single domain type 1 cystatins in the trematode fasciola gigantica that are released into the parasite's intestinal tract and exhibit inhibitory activity against endogenous and host cathepsin l and b proteases. in contrast, the here presented 170kda multi-domain cystatin (fgmdc) comprises signal peptide and 12 tandem repeated ...201425176159
liver fluke vaccines in ruminants: strategies, progress and future opportunities.the development of a vaccine for fasciola spp. in livestock is a challenge and would be advanced by harnessing our knowledge of acquired immune mechanisms expressed by resistant livestock against fluke infection. antibody-dependent cell-mediated cytotoxicity directed to the surface tegument of juvenile/immature flukes is a host immune effector mechanism, suggesting that antigens on the surface of young flukes may represent prime candidates for a fluke vaccine. a type 1 immune response shortly af ...201425200351
rnai dynamics in juvenile fasciola spp. liver flukes reveals the persistence of gene silencing in vitro.fasciola spp. liver fluke cause pernicious disease in humans and animals. whilst current control is unsustainable due to anthelmintic resistance, gene silencing (rna interference, rnai) has the potential to contribute to functional validation of new therapeutic targets. the susceptibility of juvenile fasciola hepatica to double stranded (ds)rna-induced rnai has been reported. to exploit this we probe rnai dynamics, penetrance and persistence with the aim of building a robust platform for reverse ...201425254508
specific diagnosis of opisthorchis viverrini using loop-mediated isothermal amplification (lamp) targeting parasite microsatellites.opisthorchis viverrini and other food-borne trematode infections are major health problems in thailand, the lao people's democratic republic, vietnam and cambodia. differential diagnosis of o. viverrini based on the microscopic observation of parasite eggs is difficult in areas where clonorchis sinensis and minute intestinal flukes coexist. recently, loop-mediated isothermal amplification (lamp) has been widely used for detection and identification of trematode for its simple method that is usef ...201525268466
fascioliasis: a worldwide parasitic disease of importance in travel medicine.fascioliasis is a foodborne zoonotic disease caused by the two parasite species fasciola hepatica and fasciola gigantica. this trematodiasis has never been claimed special relevance for travellers and migrants. however, the situation has drastically changed in the last two decades, in a way that fascioliasis should today be included in the list of diseases to be enhanced in travel medicine. different kind of travellers have been involved in human infection reports: business travellers, tourists, ...201425287722
identification of fasciola species based on mitochondrial and nuclear dna reveals the co-existence of intermediate fasciola and fasciola gigantica in thailand.molecular techniques were used to identify fasciola species collected from chiang mai thailand. morphometrically, 65 stained and 45 fresh worms collected from cattle suggested the possible occurrence of both f. gigantica and f. hepatica. twenty-two worms comprising 15 from cattle and 7 from human patients, were identified subsequently based on three genetic markers: mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1), mitochondrial cytochrome c oxidase subunit 1 (cox1) ...201425300764
cholestasis caused by fasciola gigantica.fascioliasis is an infectious disease caused by the hepatic trematodes fasciola hepatica and fasciola gigantica. here, we report the case of fasciola gigantica presenting with biliary obstruction and abdominal pain that was diagnosed and treated by endoscopic retrograde cholangiography (ercp). a 46-year-old woman presented with right upper quadrant abdominal pain and jaundice. physical examination revealed icterus and hepatomegaly. laboratory findings revealed an increase in liver transaminases ...201425308461
monoclonal antibody against recombinant fasciola gigantica cathepsin l1h could detect juvenile and adult cathepsin ls of fasciola gigantica.cathepsin ls (catls), the major cysteine protease secreted by fasciola spp., are important for parasite digestion and tissue invasion. fasciola gigantica cathepsin l1h (fgcatl1h) is the isotype expressed in the early stages for migration and invasion. in the present study, a monoclonal antibody (moab) against recombinant f. gigantica cathepsin l1h (rfgcatl1h) was produced by hybridoma technique using spleen cells from balb/c mice immunized with recombinant profgcatl1h (rprofgcatl1h). this moab i ...201525324133
distribution and abundance of schistosomiasis and fascioliasis host snails along the mara river in kenya and tanzania.we purposively selected 39 sampling sites along the mara river and its two perennial tributaries of amala and nyangores and sampled snails. in addition, water physicochemical parameters (temperature, turbidity, dissolved oxygen, conductivity, alkalinity, salinity and ph) were taken to establish their influence on the snail abundance and habitat preference. out of the 39 sites sampled, 10 (25.6%) had snails. the snail species encountered included biomphalaria pfeifferi krauss - the intermediate h ...201425405008
distribution of fasciola hepatica and f. gigantica in the endemic area of guilan, iran: relationships between zonal overlap and phenotypic traits.fascioliasis is a zoonotic disease emerging in numerous parts of the world. in any endemic area, the characterisation of scenarios and patterns of infection must always be considered the starting point before implementing any control measure. fascioliasis is a parasitic disease of different epidemiological, pathological and control characteristics depending on the endemic area and the causal agent, fasciola hepatica and fasciolagigantica. classically it has been accepted that f. hepatica is pres ...201525602718
high prevalence of schistosoma japonicum and fasciola gigantica in bovines from northern samar, the philippines.the cause of zoonotic schistosomiasis in the philippines is schistosoma japonicum, which infects up to 46 mammalian hosts, including humans and bovines. in china, water buffaloes have been identified as major reservoir hosts for schistosomiasis japonica, contributing up to 75% of human transmission. in the philippines, water buffaloes (carabao; bubalus bubalis carabanesis) have, historically, been considered unimportant reservoirs. we therefore revisited the possible role of bovines in schistoso ...201525643317
a comparative study of haemocytes from resistant and susceptible lymnaea natalensis snails exposed to fasciola gigantica miracidia.effect ot infection with fasciola gigantica on total and differential haemocytes count of resistant and susceptible lymnaea natalensis snails were studied. exposure of l. natalensis resistant and susceptible strains to f. gigantica on miracidia caused gradual increase in the number of circulating haemocytes at the same time of exposure. in susceptible strain, the increase in the number of circulating haemocytes became significant at the second week post exposure being 2560 cell/ml (p<o.05) in co ...201425643506
isolation and characterization of cu/zn-superoxide dismutase in fasciola gigantica.a full-length complementary dna (cdna) encoding cu/zn-superoxide dismutase was isolated from fasciola gigantica that on nucleotide sequencing showed a close homology (98.9%) with cu/zn-superoxide dismutase (sod) of the temperate liver fluke, f. hepatica. expression of the gene was found in all the three developmental stages of the parasite viz. adult, newly excysted juvenile and metacercaria at transcriptional level by reverse transcription-polymerase chain reaction (rt-pcr) and at the protein l ...201525655406
saposin-like protein 2 has an immunodiagnostic potential for detecting fasciolosis gigantica.saposin-like protein 2 (sap-2) plays an important role in the digestive process of fasciola gigantica (fg). it is one of the major proteins synthesized by the caecal epithelial cells and released into fluke's excretion-secretion. therefore, fgsap-2 is a plausible target for detecting fasciolosis. a polyclonal antibody (poab) against recombinant fgsap-2 was produced by immunizing rabbits with the recombinant protein (rfgsap-2), and used in sandwich elisa assay to detect the circulating fgsap-2 in ...201525662434
pcr-rflp analysis of 28 srdna for specification of fasciola gigantica (cobbold, 1855) in the infected lymnaea auricularia (linnaeus, 1785) snails from northwestern iran.fasciolosis in livestock is a crucial concern in the globe, mainly due to its impact on human health. the aim of this study was to determine the rate of infection with fasciola gigantica (cobbold, 1855) larvae in the field-collected snails of lymnaea auricularia (linnaeus, 1785) from northwestern iran using a molecular approach.201425678920
detection of coproantigens by sandwich elisa in rabbits experimentally infected with fasciola gigantica.the study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of fasciola gigantica experimentally infected rabbits.201425678922
genetic characterization of fasciola gigantica from different geographical regions of india by ribosomal dna markers.ribosomal dna sequences of the second internal transcribed spacer (its-2) and 28s ribosomal dna (618 bp) of fasciola gigantica collected from cattle and buffaloes from four different geographical locations of india, were characterized for genotyping. its-2 sequence was analyzed in 28 worms that was typical of f. gigantica and differed at six positions, with one of these being a distinguishing deletion (t) at the 327th position in f. gigantica relative to f. hepatica. however, fasciola specimens ...201325698855
protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l.fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ...201525701317
toxicity of chlorophyllin in different wavelengths of visible light against fasciola gigantica larvae.toxicity of chlorophyllin against redia and cercaria larvae of fasciola gigantica was studied under irradiation of visible light. highest and lowest toxicity of chlorophyllin against both larvae was noted under red (redia - 8 h lc₅₀ 7.88 mg/10 ml and cercaria - 11.99 mg/10 ml) and green (redia - 8 h lc₅₀ 32.12 mg/10 ml and cercaria - 8 h lc₅₀ 43.80 mg/10 ml) light irradiation respectively. the highest toxicity of chlorophyllin under red light irradiation against redia (8h lc₅₀ 7.88 mg/10 ml)/cer ...201525720554
experimental life history and biological characteristics of fasciola gigantica (digenea: fasciolidae).this study was conducted to investigate the life history, morphology, and maturation of larval stages and adult worms of fasciola gigantica in experimental mice. lymnaea auricularia rubiginosa was used as the intermediate host, and oryza sativa was used for encystment of the metacercariae, while mus musculus was used as the definitive host for maturation study. fresh eggs from the gall bladder of water buffaloes fully developed into embryonated ones and hatched out at days 11-12 after incubation ...201525748710
molecular evidence shows that the liver fluke fasciola gigantica is the predominant fasciola species in ruminants from pakistan.fascioliasis is an important disease affecting livestock, with great costs to producers worldwide. it has also become a serious issue for human populations in some endemic areas as an emerging zoonotic infection. there are two fasciola species of liver fluke responsible for this disease, which occur worldwide, fasciola hepatica and fasciola gigantica. identifying these two species on the basis of adult or egg morphology requires specialist knowledge due to the similarity of characters, and may m ...201625758445
molecular cloning and characterization of fasciola gigantica thioredoxin-glutathione reductase.the fasciola gigantica thioredoxin-glutathione reductase (fgtgr) gene is a fusion between thioredoxin reductase (tr) and a glutaredoxin (grx) gene. fgtgr was cloned by polymerase chain reaction (pcr) from adult complementary dna (cdna), and its sequences showed two isoforms, i.e., the cytosolic and mitochondrial fgtgr. cytosolic fgtgr (cytfgtgr) was composed of 2370 bp, and its peptide had no signal sequence and hence was not a secreted protein. mitochondrial fgtgr (mitfgtgr) was composed of 250 ...201525782678
mitochondrial genome of hypoderaeum conoideum - comparison with selected trematodes.hypoderaeum conoideum is a neglected but important trematode. the life cycle of this parasite is complex: snails serve as the first intermediate hosts: bivalves, fishes or tadpoles serve as the second intermediate hosts, and poultry (such as chickens and ducks) act as definitive hosts. in recent years, h. conoideum has caused significant economic losses to the poultry industry in some asian countries. despite its importance, little is known about the molecular ecology and population genetics of ...201525889473
molecular differentiation of fasciola species and characterization of genetic diversity of f. gigantica using nadh dehydrogenase i (nd1) gene in the endemic areas of iran.fasciola hepatica and f. gigantica are the causative agents of fasciolosis in domestic animals and humans. based on the morphometric criteria, differential diagnosis between them is problematic. in addition, intermediate forms of fasciola have been found in iran, which makes the differentiation more difficult. the aim of the present study was to provide molecular evidence for the existence of f. gigantica in iran using sequencing analysis of nd1 and pcr-rflp analysis of its2 regions and to study ...201725904941
molecular phylogenetic analysis of fasciola flukes from eastern india.fasciola flukes from eastern india were characterized on the basis of spermatogenesis status and nuclear its1. both fasciola gigantica and aspermic fasciola flukes were detected in imphal, kohima, and gantoku districts. the sequences of mitochondrial nad1 were analyzed to infer their phylogenetical relationship with neighboring countries. the haplotypes of aspermic fasciola flukes were identical or showed a single nucleotide substitution compared to those from populations in the neighboring coun ...201525936970
fasciola hepatica from naturally infected sheep and cattle in great britain are diploid.diploid (2n = 2x = 20) and triploid (2n = 3x = 30) fasciola hepatica have been reported in the uk, and in asia diploid, triploid and mixoploid (2x/3x) fasciola spp. exist but there is little information to indicate how common triploidy is, particularly in uk fluke. here the ploidy of 565 adult f. hepatica from 66 naturally infected british sheep and 150 adult f. hepatica from 35 naturally infected british cattle was determined. all 715 of these parasites were diploid, based on observation of 10 ...201525990533
somatic antigens of tropical liver flukes ameliorate collagen-induced arthritis in wistar rats.parasitic helminths polarize immune response of their vertebrate hosts towards anti-inflammatory th2 type and therefore it is hypothesized that they may suppress the inflammatory conditions in autoimmune disorders. the present study was undertaken to investigate in vivo immunomodulatory and therapeutic potential of somatic antigens (ag) of liver infecting digenetic trematodes [fasciola gigantica (fg) and gigantocotyle explanatum (ge)] in collagen-induced arthritic (cia) wistar rats. the cia rats ...201525992888
juvenile-specific cathepsin proteases in fasciola spp.: their characteristics and vaccine efficacies.fasciolosis, caused by fasciola hepatica and fasciola gigantica, is one of the most neglected tropical zoonotic diseases. one sustainable control strategy against these infections is the employment of vaccines that target proteins essential for parasites' invasion and nutrition acquiring processes. cathepsin proteases are the most abundantly expressed proteins in fasciola spp. that have been tested successfully as vaccines against fasciolosis in experimental as well as large animals because of t ...201526099239
vaccine potential of recombinant pro- and mature cathepsinl1 against fasciolosis gigantica in fasciola gigantica cathepsin l1 (catl1) is a family of predominant proteases that is expressed in caecal epithelial cells and secreted into the excretory-secretory products (es). catl1 isotypes are expressed in both early and late stages of the life cycle and the parasites use them for migration and digestion. therefore, catl1 is a plausible target for vaccination against this parasite. recombinant pro-f.gigantica catl1 (rprofgcatl1) and recombinant mature f.gigantica catl1 (rmatfgcatl1) were ...201526116785
associations between trematode infections in cattle and freshwater snails in highland and lowland areas of iringa rural district, tanzania.the epidemiology of trematode infections in cattle was investigated within highland and lowland areas of iringa rural district, in southern tanzania. fecal samples were collected from 450 cattle in 15 villages at altitudes ranging from 696 to 1800 m above the sea level. freshwater snails were collected from selected water bodies and screened for emergence of cercariae. the infection rates in cattle were fasciola gigantica 28·2%, paramphistomes 62·8% and schistosoma bovis 4·8%. notably, prevalenc ...201526152614
[cloning, expression and immunodiagnostic evaluation of the fasciola gigantica thioredoxin peroxidase].to immunoscreen the gene encoding thioredoxin peroxidase (tpx) from a cdna library made from adult fasciola gigantica worms, clone and express the gene, and evaluate the immunodiagnostic value of tpx recombinant protein.201526245114
serological and coprological analyses for the diagnosis of fasciola gigantica infections in bovine hosts from sargodha, pakistan.a serological and coprological survey of fasciolosis was conducted in bovine hosts from the sargodha district, pakistan using excretory-secretory (es) antigens of fasciola gigantica from cattle and buffaloes. livers, faecal and blood samples of 146 cattle and 184 buffaloes were collected from slaughterhouses and examined for the presence of any fasciola in bile ducts and ova in faeces. serum was separated. es antigens were prepared by incubating adult fasciola in phosphate-buffered saline for 6- ...201626300295
a case of fascioliasis treated successfully without sequelae in a japanese expatriate living in jakarta.a 46-year-old japanese female expatriate living in jakarta presented with intermittent fever lasting for a month. although she was considered at low risk of fasciola spp. infection because she lived in an upper-class residential area of the city, the patient presented with eosinophilia after consuming organic raw vegetables; in addition, contrast-enhanced computed tomography detected microabscesses in a tractlike pattern in the liver. these findings led to an early diagnosis of fascioliasis, whi ...201526309420
molecular characterization of veterinary important trematode and cestode species in the mithun bos frontalis from north-east india.helminth infections in the mithun bos frontalis, including the liver fluke fasciola gigantica, hepato-gastric amphistomes explanatum explanatum, paramphistomum epiclitum and calicophoron calicophorum, and the cestodes echinococcus granulosus and e. ortleppi were studied in north-east india over a 2-year period from 2012 to 2014. cystic echinococcosis caused by e. granulosus and e. ortleppi was found to be highly prevalent in the mithun, with e. ortleppi being reported for the first time. molecul ...201626343353
diagnotic value of some fasciola gigantica antigens.the present study was aimed to select the specificity of antigens for fasciola gigantica depending on its diagnostic utility and field applications. the tested antigens were coproantigen, excretory-secretory (es) antigen and egg antigen. coproantigen and copro hyperimmune serum were able to reflect the lowest level of cross-reaction with other tested f. gigantica antigens. by using sds-page, a structural homology was observed in f. gigantica es and egg antigens. intense cross reaction was observ ...201526345056
significance of a common 65 kda antigen in the experimental fasciolosis and the current study, cross-reaction between two important zoonotic parasites; extracellular helminthes fasciola gigantica and intracellular protozoa toxoplasma gondii was proved by enzyme linked immunosorbent assay. five antigens were used to identify and compare the cross-binding activities in the prepared antisera. two f. gigantica antigens; adult flukes (fga) and eggs (fgea) were used to detect igg in t. gondii naturally infected human sera (tgihs) and experimentally infected sera of sheep ( ...201526345069
biliary fasciola gigantica infestation in a nonendemic area--an intraoperative surprise.a 7year old girl infected with the zoonotic trematode, fasciola gigantica is reported because of the extreme rarity of this condition in our region. because of the overlap in symptomatology and radiological features, the more common amebic/pyogenic liver abscess in the initial hepatic migratory phase and later choledochal cyst/biliary ascariasis when the parasite was finally located in the extrahepatic bile ducts, were thought of delaying effective treatment. the diagnosis was confirmed only by ...201526362003
clinical research abstracts of the british equine veterinary association congress 2015.although fasciolosis is an important livestock disease worldwide, the public health importance of human fasciolosis has increased in recent years and it is recognised as an important re-emerging zoonotic disease, its epidemiology and pathogenicity in donkeys, and the epidemiological role they may play have not been determined.201526375729
preferential regeneration of thioredoxin from parasitic flatworm fasciola gigantica using glutathione system.the maintenance of cellular redox homeostasis is a crucial adaptive problem faced by parasites, and its disruption can shift the biochemical balance toward the host. the thioredoxin (trx) system plays a key role in redox metabolism and defense against oxidative stress. in this study, biochemical experiments were performed on fasciola gigantica thioredoxin1 (fgtrx1). the recombinant fgtrx1 exists as a monomer and catalyzes the reduction of insulin. fgtrx1 is preferentially regenerated by the glut ...201526408792
fasciola gigantica cathepsin b5 is an acidic endo- and exopeptidase of the immature and mature parasite.cysteine proteases of the liver fluke fasciola have been described as essential molecules in the infection process of the mammalian host. destinct cathepsin bs, which are already expressed in the metacercarial stage and released by the newly excysted juvenile are major actors in this process. following infection their expression is stopped and the proteins will not be detectable any longer after the first month of development. on the contrary, the novel cathepsin b5 of fasciola gigantica (fgcb5) ...201526453811
comparing an in vivo egg reduction test and in vitro egg hatching assay for different anthelmintics against fasciola species, in cattle.this study aimed to compare between the efficiency of in vivo fecal egg reduction test (fert) and in vitro egg hatching assay (eha) in evaluating of the anti-fasciola activity of albendazole, triclabendazole, oxyclozanide and praziquantel. a field trial was carried out on fifty naturally fasciola infected cattle that were divided equally into 5 groups (a-e). on day zero; groups a-d were drenched with albendazole, triclabendazole, oxyclozanide or praziquantel, respectively, while the remaining on ...201526455573
dna sequence analyses reveal co-occurrence of novel haplotypes of fasciola gigantica with f. hepatica in south africa and zimbabwe.the aim of this study was to identify and determine the genetic diversity of fasciola species in cattle from zimbabwe, the kwazulu-natal and mpumalanga provinces of south africa and selected wildlife hosts from zimbabwe. this was based on analysis of dna sequences of the nuclear ribosomal internal transcribed spacer (its1 and 2) and mitochondrial cytochrome oxidase 1 (co1) regions. the sample of 120 flukes was collected from livers of 57 cattle at 4 abattoirs in zimbabwe and 47 cattle at 6 abatt ...201526476916
inhibition of acetylcholinesterase and cytochrome oxidase activity in fasciola gigantica cercaria by phytoconstituents.fasciolosis is an important cattle and human disease caused by fasciola hepatica and fasciola gigantica. one of the possible methods to control this problem is to interrupt the life cycle of fasciola by killing its larva (redia and cercaria) in host snail. molecular identification of cercaria larva of f. gigantica was done by comparing the nucleotide sequencing with adult f. gigantica. it was noted that nucleotide sequencing of cercaria larva and adult f. gigantica were 99% same. every month dur ...201626536397
monitoring of fasciola species contamination in water dropwort by cox1 mitochondrial and its-2 rdna sequencing analysis.fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. water dropwort (oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of fasciola, and the fresh stems and leaves are widely used as a seasoning in the korean diet. however, no information regarding fasciola species contamination in water dropwort is available. here, we collected 500 samples of water dropwort in 3 areas in korea during february and m ...201526537044
current status of fasciolosis in vietnam: an update and perspectives.vietnam is recognized to be endemic for fasciolosis. however, most of the available publications have not been published in international journals. this review is based on national and international vietnamese publications and highlights the current status of fasciolosis in vietnam. it also provides some information available for neighbouring countries. updated data on responsible species, distribution, transmission and control aspects are summarized. the central region of vietnam is reported as ...201626564097
partial hepatectomy for the resistant fasciola hepatica infection in a child.fascioliasis is an emerging and important chronic parasitic disease caused by two trematode liver fluke species: fasciola hepatica (f. hepatica) and fasciola gigantica (f. gigantica) infecting several herbivorous mammals including cattle, goats, sheep, and humans. we report a 9-year-old girl who suffered from f. hepatica infection and underwent right hepatectomy because of increasing abdominal pain resistant to anthelmintic chemotherapy. when anthelmintic drug treatment is not effective and abdo ...201726623254
novel methods for the molecular discrimination of fasciola spp. on the basis of nuclear protein-coding genes.fasciolosis is an economically important disease of livestock caused by fasciola hepatica, fasciola gigantica, and aspermic fasciola flukes. the aspermic fasciola flukes have been discriminated morphologically from the two other species by the absence of sperm in their seminal vesicles. to date, the molecular discrimination of f. hepatica and f. gigantica has relied on the nucleotide sequences of the internal transcribed spacer 1 (its1) region. however, its1 genotypes of aspermic fasciola flukes ...201626680160
biochemical assessment of oxidative status versus liver enzymes in patients with chronic fascioliasis.the aim of this study was to examine the oxidative status in egyptian patients suffering chronic fascioliasis. the relationship between serum malondialdehyde (mda) levels, superoxide dismutase (sod), catalase (cat), and glutathione peroxidase (gpx) activities was investigated in relation to the level of liver enzymes; alt and ast compared to healthy controls. twenty patients versus ten controls were included in the study. among cases the mda, cat, ast and alt were higher than controls, while sod ...201526688624
an enzyme-linked immunosorbent assay for diagnosis of fasciola gigantica infection in cattle and buffaloes.the enzyme-linked immunosorbent assay (elisa) was evaluated for the diagnosis of fasciola gigantica infection in cattle and buffaloes. the excretory-secretory (e-s ag) antigen of f. gigantica adult flukes obtained after invitro incubation was used as an antigen. the test was conducted with 276 sera collected from cattle and buffaloes which included 22 sera each from naturally infected cattle and buffaloes (known positive serum) and with similar number of samples with healthy cattle and buffaloes ...201526688653
in vitro screening of cymbopogon jwarancusa and conyza canadensis against liver flukes.aim of present study was to screen medicinal plants for flukicidal activity in vitro to develop alternative sources of treatment for trematodes infection. for this purpose, crude methanolic extracts (cme) of cymbopogn jwarancusa and conyza canadensis were prepared and live adult flukes viz; fasciola gigantica, and paramphistomum cervi isolated from liver and bile ducts of slaughtered buffalo were subjected to different drug concentrations as well as positive and negative control. motility inhibi ...201526695200
immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich elisa and immunochromatographic assay for detection of circulating cathepsin l1 protease.tropical fasciolosis caused by fasciola gigantica infection is one of the major diseases infecting ruminants in the tropical regions of africa and asia including thailand. parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. therefore, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden.201626731402
higher physiopathogenicity by fasciola gigantica than by the genetically close f. hepatica: experimental long-term follow-up of biochemical markers.fascioliasis is caused by fasciola hepatica and f. gigantica. the latter, always considered secondary in human infection, nowadays appears increasingly involved in africa and asia. unfortunately, little is known about its pathogenicity, mainly due to difficulties in assessing the moment a patient first becomes infected and the differential diagnosis with f. hepatica.201626740363
a singleplex real-time fluorescence resonance energy transfer pcr with melting curve analysis for the differential detection of paragonimus heterotremus, echinostoma malayanum and fasciola gigantica eggs in faeces.because the eggs of paragonimus, echinostoma and fasciola are very similar in size and shape, it is difficult to distinguish and accurately identify species by the morphology of their eggs, which is a standard diagnostic method.201626740365
screening for zoonotic fascioliasis in slaughtered large ruminants in abattoirs in perak, malaysia.fascioliasis, or trematode infestation, is an important disease caused by fasciola hepatica and fasciola gigantica. both species are hepatic parasites that affect humans. we have examined the zoonotic aspects of fascioliasis. a total of 80 fresh liver samples were collected from 67 kedah-kelantan crossbred cattle and 13 murrah buffalo at 4 local abattoirs in perak, malaysia. the samples were examined macroscopically to detect the presence of fasciola spp. the results show 7.50% (6 of 80) of the ...201526868715
Displaying items 601 - 700 of 754