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candida haemulonii from clinical specimens in the usa.classical yeast identification procedures and dna relatedness studies confirmed the occurrence of candida haemulonii among clinical specimens in the usa, particularly isolations from the foot. none of the six clinical isolates studied produced identical api 20c profile codes.19911955954
transient fungemia caused by an amphotericin b-resistant isolate of candida haemulonii.a bloodstream infection due to candida haemulonii afflicting a patient with fever and a medical history of megaloblastic anemia is reported. the clinical isolate was misidentified by the api 20c and vitek identification systems. the results of susceptibility tests showed that the mic of amphotericin b for c. haemulonii was 4 microg/ml. additional susceptibility testing procedures based on the use of antibiotic medium 3 and iso-sensitest broth were performed, and killing curves were determined. t ...200212037106
torulopsis haemulonii nov. spec., a yeast from the atlantic ocean. 196214039875
candida alocasiicola sp. nov., candida hainanensis sp. nov., candida heveicola sp. nov. and candida musiphila sp. nov., novel anamorphic, ascomycetous yeast species isolated from plants.in a taxonomic study on the ascomycetous yeasts isolated from plant materials collected in tropical forests in yunnan and hainan provinces, southern china, four strains isolated from tree sap (yj2e(t)) and flowers (yf9e(t), ywzh3c(t) and yyf2a(t)) were revealed to represent four undescribed yeast species. molecular phylogenetic analysis based on the large subunit (26s) rrna gene d1/d2 domain sequences showed that strain yj2e(t) was located in a clade together with candida haemulonii and c. pseud ...200818344072
first three reported cases of nosocomial fungemia caused by candida auris.candida auris is a newly described species whose clinical significance is not clear. here, we describe the first three cases of nosocomial fungemia caused by c. auris, which confirms that it is a causative agent of bloodstream infections. all three patients presented persistent fungemia for 10 to 31 days. the isolates obtained from the three patients were misidentified as candida haemulonii and rhodotorula glutinis by the vitek 2 and the api 20c systems, respectively. c. auris was confirmed by s ...201121715586
multidrug-resistant candida haemulonii and c. auris, tel aviv, israel.candida auris and c. haemulonii are closely related, multidrug-resistant emerging fungal pathogens that are not readily distinguishable with phenotypic assays. we studied c. auris and c. haemulonii clinical isolates from 2 hospitals in central israel. c. auris was isolated in 5 patients with nosocomial bloodstream infection, and c. haemulonii was found as a colonizer of leg wounds at a peripheral vascular disease clinic. liberal use of topical miconazole and close contact among patients were imp ...201728098529
can multidrug-resistant candida auris be reliably identified in clinical microbiology laboratories? 201727881617
susceptibility to antifungal agents and enzymatic activity of candida haemulonii and cutaneotrichosporon dermatis isolated from soft corals on the brazilian reefs.candida is a common fungus with the capacity to cause infections in humans. however, most studies have concentrated on clinical isolates and little is known about the identity, ecology and drug resistance of free living species/strains. here, we isolate eight strains of candida haemulonii and four strains of cutaneotrichosporon dermatis from three marine cnidarian zoanthids species (palythoa caribaeorum, palythoa variabilis and zoanthus sociatus) collected from brazilian coral reefs. strains wer ...201627282152
nosocomial fungemia by candida auris: first four reported cases in continental europe.candida auris is an emerging multidrug-resistant yeast that can cause invasive infections and is associated with high mortality. it is typically resistant to fluconazole and voriconazole and, some cases, also to echinocandins and amphotericin b. this species, phylogenetically related to candida haemulonii, is frequently misidentified by commercial identification techniques in clinical laboratories; therefore, the real prevalence of c. auris infections may be underestimated.201728131716
planktonic growth and biofilm formation profiles in candida haemulonii species complex. 201728159990
multidrug-resistant candida auris misidentified as candida haemulonii: characterization by matrix-assisted laser desorption ionization-time of flight mass spectrometry and dna sequencing and its antifungal susceptibility profile variability by vitek 2, clsi broth microdilution, and etest method.candida auris is a multidrug-resistant yeast that causes a wide spectrum of infections, especially in intensive care settings. we investigated c. auris prevalence among 102 clinical isolates previously identified as candida haemulonii or candida famata by the vitek 2 system. internal transcribed spacer region (its) sequencing confirmed 88.2% of the isolates as c. auris, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) easily separated all related sp ...201525809970
protease and phospholipase activities of candida spp. isolated from cutaneous candidiasis.cases of superficial and invasive mycoses caused by emerging species of candida have been increasingly reported over the last thirty years. the production of hydrolytic enzymes plays a central role in the fungal infective process. in candida infections the secretion of both proteases and phospholipases are well-known virulence attributes.201524853474
candida haemulonii complex species, brazil, january 2010-march 2015. 201626891028
primary deep cutaneous candidiasis caused by candida duobushaemulonii in a 68-year-old man: the first case report and literature review.superficial candida infections of the skin are common, but deep cutaneous candidiasis, including secondary dissemination to the skin from systemic candidiasis, candidaemia or primary invasion due to skin defects such as trauma, is rare. these patients are usually immunosuppressed, but immunocompetent hosts can be affected as well. candida albicans is the most common pathogen. however, non-albicans candida species can cause deep skin invasion in rare circumstances. we report a case of deep cutane ...201627600646
species distribution and susceptibility profile of yeasts isolated from blood cultures: results of a multicenter active laboratory-based surveillance study in argentina.the mycology department of the instituto nacional de enfermedades infecciosas "dr. c. malbrán", conducted the second national multicenter survey on fungemia due to yeasts in argentina. the aim was to obtain updated data of the frequency of the causative species encountered and their in vitro susceptibility to seven antifungal agents. yeast species were identified by micromorphological and biochemical studies. antifungal susceptibility testing was performed by the reference microdilution method e ...201122430989
a case of candida haemulonii osteitis: clinical features, biochemical characteristics, and antifungal resistance profile.we describe here the first case of osteitis caused by candida haemulonii in a young immunocompetent patient. this patient presented a history of severe peripheral vascular disease associated with a lack of hygienic conditions as the only risk factors for such an uncommon infection. clinical signs and histological examination allowed us to determine that it was a c. haemulonii infection and not colonization. the outcome was favourable with oral voriconazole therapy and surgical revascularization. ...201121375662
rare occurrence of fatal candida haemulonii peritonitis in a diabetic capd patient.a 68-year-old diabetic chronic kidney disease patient on continuous ambulatory peritoneal dialysis for two years developed candida haemulonii peritonitis without any predisposing factors. there is no effective treatment for this fungus. a peritoneal biopsy showed morphological changes of acute inflammation and chronicity.201425076189
multidrug-resistant endemic clonal strain of candida auris in india.candida auris is a recently described rare agent of fungemia. it is notable for its antifungal resistance. a total of 15 c. auris isolates, originating from seven cases of fungemia, three cases of diabetic gangrenous foot, and one case of bronchopneumonia from a tertiary care hospital in south india, were investigated. all of the 15 isolates were identified by sequencing and 14 of these along with 12 c. auris isolates previously reported from two hospitals in delhi, north india, two each from ja ...201424357342
first report of a clinical isolate of candida haemulonii in brazil. 201223070353
catheter-related candidemia caused by candida haemulonii in a patient in long-term hospital care.candida haemulonii, one of the non-albicans candida species, is an emerging yeast pathogen that is known to be resistant to amphotericin b and other antifungal agents such as azoles. these anti-fungal agents have often been associated with clinical treatment failure, so no treatment regimen has been clearly established for invasive c. haemulonii infections. we investigated a catheter-related infection of c. haemulonii candidemia in an adult patient in long-term hospital care. in the early stages ...201121286025
molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, penaeus monodon, and its expression profile in response to various immunostimulants and challenge with wssv.a crustin-like antimicrobial peptide from the haemocytes of giant tiger shrimp, penaeus monodon was partially characterized at the molecular level and phylogenetic analysis was performed. the partial coding sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic of the shrimp crustins. phylogenetic tree and sequence comparison clearly confirmed divergence of this crustin-like amp from other shrimp crustins. the differential expression of the cru ...201120580462
detection of amphotericin b resistance in candida haemulonii and closely related species by use of the etest, vitek-2 yeast susceptibility system, and clsi and eucast broth microdilution methods.the emerging fungal pathogens candida haemulonii and candida pseudohaemulonii often show high-level resistance to amphotericin b (amb). we compared the utilities of five antifungal susceptibility testing methods, i.e., the etest using mueller-hinton agar supplemented with glucose and methylene blue (etest-mh), the etest using rpmi agar supplemented with glucose (etest-rpg), the vitek-2 yeast susceptibility system, and the clinical and laboratory standards institute (clsi) and european committee ...201222442324
biofilm formation and genotyping of candida haemulonii, candida pseudohaemulonii, and a proposed new species (candida auris) isolates from korea.emergence of candida haemulonii and closely related species at five korean hospitals has been recently described. we examined biofilm formation by these isolates and assessed their genotypic relatedness by pulsed-field gel electrophoresis (pfge). this study is the first to show that all bloodstream isolates of candida pseudohaemulonii can form significant biofilms in glucose-containing medium. pfge of noti-digested genomic dna revealed that c. pseudohaemulonii isolates recovered from seven patie ...201120560864
infections due to candida haemulonii: species identification, antifungal susceptibility and outcomes.here we report the clinical features and treatment outcomes of three patients with candida haemulonii infection. candida haemulonii was confirmed by sequence analysis of the internal transcribed spacer (its) regions of the rrna genes and the 18s rrna genes. two of the three isolates were associated with fungaemia and reduced susceptibility to fluconazole [minimum inhibitory concentrations (mics) of 16 mg/l] and amphotericin b (mics of 2 mg/l). however, one of these two patients responded to fluc ...201019786341
candida haemulonii and closely related species at 5 university hospitals in korea: identification, antifungal susceptibility, and clinical features.background. candida haemulonii, a yeast species that often exhibits antifungal resistance, rarely causes human infection. during 2004-2006, unusual yeast isolates with phenotypic similarity to c. haemulonii were recovered from 23 patients (8 patients with fungemia and 15 patients with chronic otitis media) in 5 hospitals in korea. methods. isolates were characterized using d1/d2 domain and its gene sequencing, and the susceptibility of the isolates to 6 antifungal agents was tested in vitro. res ...200919193113
candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a japanese hospital.a single strain of a novel ascomycetous yeast species belonging to the genus candida was isolated from the external ear canal of an inpatient in a japanese hospital. analyses of the 26s rdna d1/d2 domain, nuclear ribosomal dna its region sequences, and chemotaxonomic studies indicated that this strain represents a new species with a close phylogenetic relationship to candida ruelliae and candida haemulonii in the metschnikowiaceae clade. this strain grew well at 40 degrees c, but showed slow and ...200919161556
candida ruelliae sp. nov., a novel yeast species isolated from flowers of ruellia sp. (acanthaceae).two novel yeast strains designated as 16q1 and 16q3 were isolated from flowers of the ruellia species of the acanthaceae family. the d1/d2 domain and its sequences of these two strains were identical. sequence analysis of the d1/d2 domain of large-subunit rrna gene indicated their relationship to species of the candida haemulonii cluster. however, they differ from c. haemulonii by 14% nucleotide sequence divergence, from candida pseudohaemulonii by 16.1% and from c. haemulonii type ii by 16.5%. ...200818422614
reverse line blot hybridization assay for identification of medically important fungi from culture and clinical specimens.we evaluated a combined panfungal pcr-reverse line blot (rlb) hybridization assay based on internal transcribed spacer 1 (its1) and its2 region polymorphisms to identify 159 candida, cryptococcus neoformans, and aspergillus isolates (22 species). its utility to identify fungal pathogens directly from 27 clinical specimens was also determined. its sequence analysis was performed to resolve discrepant identifications or where no rlb result was obtained. species-specific its2- and its1-based probes ...200717634313
outbreak of fungemia among neonates caused by candida haemulonii resistant to amphotericin b, itraconazole, and fluconazole.the first outbreak of candida haemulonii fungemia is described. the seven isolates from the blood of four neonates were identified by dna sequencing of the ribosomal dna. they were all resistant to amphotericin b, fluconazole, and itraconazole. this report highlights the emergence of c. haemulonii as an opportunistic pathogen in immunocompromised patients.200717428940
kodamaea ohmeri isolates from patients in a university hospital: identification, antifungal susceptibility, and pulsed-field gel electrophoresis analysis.data on clinical isolates of kodamaea (pichia) ohmeri, an emerging fungal pathogen, are scarce. over the past 5 years, we identified yeast isolates from nine patients with fungemia as k. ohmeri by using the api 20c system. here, we reanalyzed these isolates first by sequencing the internal transcribed spacer 2 (its2) regions and then by growing the isolates on chromagar candida medium and subjecting them to pulsed-field gel electrophoresis (pfge). based on their its2 sequences, six of the nine i ...200717251396
candida pseudohaemulonii sp. nov., an amphotericin b-and azole-resistant yeast species, isolated from the blood of a patient from thailand.candida haemulonii(types i and ii) is rarely isolated from clinical specimens. we isolated a strain that is phylogenetically close to c. haemulonii from the blood of a thai patient, and named it c. pseudohaemulonii sp. nov. (cbs 10099t = jcm 12453t = dmst 17134t). the new species and c. haemulonii types i and ii were resistant to amphotericin b and azole agents but were susceptible to a 1,3-beta-d-glucan synthetase inhibitor, micafungin, and 5-flucytosine. the species were easily distinguished u ...200616785719
mortality caused by experimental infection with the yeast candida haemulonii in the adults of ornithodoros moubata (acarina: argasidae).a relatively high rate of mortality among engorged females of ornithodoros moubata (murray, 1877) was observed in our laboratory colony. the general aim of the study was to identify the causative agent responsible for this mortality. the diagnostic tests were performed by yeast identification service (cbs-delft, netherlands) and the pathogen was identified as the yeast candida haemulonii (van uden et kolipinski, 1962) meyer et yarrovi, 1978. the artificial infection study was performed by intrah ...200111437130
candida aquaetextoris sp. nov., a new species of yeast occurring in sludge from a textile industry wastewater treatment plant in tuscany, italy.we describe candida aquaetextoris, a new yeast species isolated from sludge that accumulates at the main wastewater treatment facility which processes discharges from textile factories located in the prato metropolitan district, northern tuscany, italy. this yeast degrades 4-(1-nonyl)phenol, a toxic intermediate originating from the microbial attack of nonylphenol polyethoxylates, which are nonionic surfactants largely used in leather and textile industries. in the investigation we employed conv ...19979103618
unrelatedness of groups of yeasts within the candida haemulonii complex.isoenzyme and protein profiles of clinical isolates initially identified as candida haemulonii demonstrated the presence of two distinct groups. dna relatedness studies with representative cultures confirmed the presence of two species. physiological features that can be used to separate two groups within c. haemulonii are reported.19938349743
identification of candida haemulonii complex species: use of clinprotools(tm) to overcome limitations of the bruker biotyper(tm), vitek ms(tm) ivd, and vitek ms(tm) ruo databases.candida haemulonii is now considered a complex of two species and one variety: c. haemulonii sensu stricto, candida duobushaemulonii and the variety c. haemulonii var. vulnera. identification (id) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species id for these emergent pathogens. hence, we evaluated the maldi-tof ms performance for the id of c. haemulonii species, analyzin ...201627379069
candida vulturna pro tempore sp. nov., a dimorphic yeast species related to the candida haemulonis species complex isolated from flowers and clinical sample.in a taxonomic study of yeasts isolated from flowers in cagayan de oro, mindenao island, the philippines, strains were identified as representing kabatiella microsticta, metschnikowia koreensis and a hitherto undescribed dimorphic species. sequences of the d1/d2 domains of the lsu 26s rrna genes, the internal transcribed spacer (its) regions and the ssu 18s rrna genes were identical in the strains of the last-named group and differed from the corresponding sequences of the type strain of the clo ...201627411802
identification and antifungal susceptibility profiles of candida haemulonii species complex clinical isolates from a multicenter study in china.candida haemulonii complex (candida haemulonii, candida haemulonii var. vulnera, and candida duobushaemulonii) consists of emerging pathogens. thirty-one isolates from 14 hospitals in china were studied for their species classification and antifungal susceptibilities. performances of molecular (i.e., ribosomal dna [rdna] internal transcribed spacer [its] sequencing, d1/d2 sequencing, and its sequencer-based capillary gel electrophoresis [scge]) and phenotypic identification methods in species id ...201627535688
different classes of hydrolytic enzymes produced by multidrug-resistant yeasts comprising the candida haemulonii complex.the production of enzymes in clinical isolates of candida haemulonii (ch, n = 5), candida duobushaemulonii (cd, n = 4) and candida haemulonii var. vulnera (chv, n = 3) was identified by agar plate. aspartic protease, phytase, caseinolytic and hemolytic activities were detected in all the isolates. a distinct scenario was evidenced regarding the production of lipases. in this way, 80%, 50% and 100% of ch, cd and chv, respectively, were phospholipase producers. regarding esterase activity, 100%, 5 ...201727555559
identification of yeasts isolated from raffia wine (raphia hookeri) produced in côte d'ivoire and genotyping of saccharomyces cerevisiae strains by pcr inter-delta.raffia wine is a traditional alcoholic beverage produced in several african countries where it plays a significant role in traditional customs and population diet. alcoholic fermentation of this beverage is ensured by a complex natural yeast flora which plays a decisive role in the quality of the final product. this present study aims to evaluate the distribution and the diversity of the yeast strains isolated in raffia wine from four sampling areas (abengourou, alépé, grand-lahou and adzopé) in ...201627339306
candida haemulonii species complex: an emerging species in india and its genetic diversity assessed with multilocus sequence and amplified fragment-length polymorphism analyses. 201627222324
[clinical analysis of 8 cases of neonatal septicemia caused by candida haemulonii in neonatal intensive care unit].to study the clinical characteristics, antibiotics sensitivity, outcome and risk factors of neonatal septicemia caused by candida haemulonii.201626957065
neonatal candidemia caused by candida haemulonii: case report and review of literature.candidemia is a frequent condition in neonatal intensive care units (nicu) and usually complicates the newborns clinical course. several factors are responsible for candidiasis, such as prematurity and use of broad-spectrum antibiotics, and in these cases, there are the involvement of various candida species, as c. albicans, and c. parapsilosis. however, other species as c. haemulonii has been rarely described in candidemia cases, being considered an emergent pathogen. thus, we report a case of ...201525666389
immune gene expression profile of penaeus monodon in response to marine yeast glucan application and white spot syndrome virus challenge.immunostimulant potential of eight marine yeast glucans (yg) from candida parapsilosis r20, hortaea werneckii r23, candida spencermartinsiae r28, candida haemulonii r63, candida oceani r89, debaryomyces fabryi r100, debaryomyces nepalensis r305 and meyerozyma guilliermondii r340 were tested against wssv challenge in penaeus monodon post larvae (pl). structural characterization of these marine yeast glucans by proton nuclear magnetic resonance (nmr) indicated structures containing (1-6)-branched ...201525555812
candida haemulonii complex: species identification and antifungal susceptibility profiles of clinical isolates from brazil.the emerging fungal pathogens comprising the candida haemulonii complex (candida haemulonii, candida haemulonii var. vulnera and candida duobushaemulonii) are notable for their antifungal resistance. twelve isolates with phenotypic similarity to c. haemulonii were recovered from patients in brazilian hospitals. here we aimed to identify these isolates by a molecular approach, using the current classification of this fungal complex, and to evaluate their antifungal susceptibility profiles.201525134720
reclassification of the candida haemulonii complex as candida haemulonii (c. haemulonii group i), c. duobushaemulonii sp. nov. (c. haemulonii group ii), and c. haemulonii var. vulnera var. nov.: three multiresistant human pathogenic yeasts.the candida haemulonii species complex is currently known as c. haemulonii groups i and ii. here we describe c. haemulonii group ii as a new species, candida duobushaemulonii sp. nov., and c. haemulonii var. vulnera as new a variety of c. haemulonii group i using phenotypic and molecular methods. these taxa and other relatives of c. haemulonii (i.e., candida auris and candida pseudohaemulonii) cannot be differentiated by the commercial methods now used for yeast identification. four isolates (c. ...201222952266
echinocandin resistance in two candida haemulonii isolates from pediatric patients.we report 3 cases of patients with candida haemulonii isolates that were obtained from hemocultures. in 2 of the 3 cases, isolates exhibited resistance to echinocandins and fluconazole. this is the first report of an echinocandin-resistant species of this fungus in pediatric patients.201222895037
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