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genotyping of mycobacterium avium subsp. avium isolates from naturally infected lofts of domestic pigeons in ahvaz by is901 rflp.avian tuberculosis is one of the most important infections affecting most species of birds. mycobacterium avium can not only infect all species of birds, but also infect some domesticated mammals. the most crucial aspect of control and eradication scheme is identification of infection sources and transmission routs. molecular techniques such as restriction fragment length polymorphism and pulse field gel electrophoresis have been shown to be much more discriminatory and suitable for use in the e ...201526719782
multidrug therapy of mycobacterium avium subsp. avium infection in experimentally inoculated budgerigars (melopsittacus undulatus).the aim of the study was to determine whether the four-month experimental therapy of mycobacteriosis in budgerigars may cause a complete recovery. a group of nine budgerigars was infected with a mycobacterium avium subsp. avium isolate with proven pathogenicity for budgerigars. five weeks post-inoculation, multidrug therapy was started. another group comprising six birds received the same treatment but no infection, and the third group also comprising six birds was kept without infection or trea ...201526364975
disseminated mycobacteriosis manifesting as paraplegia in two parma wallabies (macropus parma) naturally exposed to mycobacterium avium.two captive female parma wallabies (macropus parma) died after a history of flaccid paraplegia. on postmortem examination, granulomatous and suppurative osteomyelitis involving the left ischium and the lumbosacral region, with meningeal extension at the cauda equina, and caseonecrotic mastitis were the most significant changes. multiple small nodules in the liver and spleen, and an enlargement of some lymph nodes with central caseous necrosis were also observed. microscopically, a disseminated g ...201526450834
mucocutaneous inflammatory pseudotumours in simultaneous mycobacterium avium subsp. avium and mycobacterium avium subsp. hominissuis infection in a cat.mycobacterial spindle cell 'pseudotumour' has been described only once in cats. this unique proliferation of spindle-shaped histiocytes containing mycobacterium avium is associated with extensive subcutaneous lesions. we report mycobacterial pseudotumour with invasion of muscular and subcutaneous tissues in a 1-year-old female domestic longhair cat. lesions involved the facial muscles and nasal cavity, making surgical excision impossible. necropsy examination revealed additional nodules in the s ...201526292770
molecular identification of mycobacterium avium subsp. silvaticum by duplex high-resolution melt analysis and subspecies-specific real-time pcr.accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. the subspecies of mycobacterium avium have different attributes but coincide in their zoonotic potential. our knowledge about m. avium subsp. silvaticum is limited, since its identification is uncertain. mycobacterium avium subsp. avium and m. avium subsp. silvaticum can be discriminated from each other based only on phenotypic characteristics, as th ...201525740770
multifocal respiratory and vertebral mycobacteriosis in a red-tailed hawk (buteo jamaicensis).an adult, female, free-ranging red-tailed hawk (buteo jamaicensis) was presented to a rehabilitation facility for an inability to stand. on examination, it displayed bilateral exaggeration of the pelvic limb reflexes with extensor muscle rigidity, intact superficial pain response, and positive withdrawal reflexes. a complete blood count identified moderate leukocytosis characterized by moderate heterophilia. no abnormalities were appreciable on radiographic evaluation. after initial improvement, ...201525831590
detection of mycobacterium avium subspecies in the gut associated lymphoid tissue of slaughtered rabbits.rabbits are susceptible to infection by different species of the genus mycobacterium. particularly, development of specific lesions and isolation of mycobacterium avium subsp. avium and mycobacterium avium subsp. paratuberculosis, both subspecies of the m. avium complex, has been reported in wildlife conditions. although, rabbit meat production worldwide is 200 million tons per year, microbiological data on this source of meat is lacking and more specifically reports of mycobacterial presence in ...201526063469
[production and evaluation of a purified protein derivative from an argentine strain of mycobacterium avium subsp. paratuberculosis].purified protein derivatives (ppds) are non-defined antigens prepared from mycobacteria cultures. they are usually employed to evaluate the specific cellular immune response both in animals and humans. bovine and avian ppds are usually employed as antigens in mycobacterial infections such as tuberculosis and paratuberculosis. nevertheless, ppd from mycobacterium avium subsp. paratuberculosis, (ppdj) is neither commonly used nor frequently available. however, ppd from mycobacterium avium subsp. a ...201523102462
molecular analysis and miru-vntr typing of mycobacterium avium subsp. avium, 'hominissuis' and silvaticum strains of veterinary origin.besides mycobacterium avium subsp. paratuberculosis (map), m. avium subsp. avium (maa), m. avium subsp. silvaticum (mas), and 'm. avium subsp. hominissuis' (mah) are equally important members of m. avium complex, with worldwide distribution and zoonotic potential. genotypic discrimination is a prerequisite to epidemiological studies which can facilitate disease prevention through revealing infection sources and transmission routes. the primary aim of this study was to identify the genetic divers ...201626964909
mycobacterial infections in rabbits: from the wild to the laboratory.tuberculous mycobacterial diseases such as leprosy and tuberculosis are ancient diseases that currently continue threatening human health in some countries. non-tuberculous mycobacterial (ntm) infections cause a series of well-defined pathological entities, as well as some opportunistic diseases that have also increased worldwide, being more common among immunocompromised patients but rising also in immunocompetent individuals. reports on natural infections by mycobacteria in rabbits are scarce ...201626799551
presence of mycobacterium avium subsp. paratuberculosis in alpacas (lama pacos) inhabiting the chilean altiplano.mycobacterium avium subsp. paratuberculosis (map) is the etiologic agent of paratuberculosis. the organism causes disease in both domestically managed and wild ruminant species. south american camelids have a long, shared history with indigenous people in the andes. over the last few decades, increasing numbers of alpacas were exported to numerous countries outside south america. no paratuberculosis surveillance has been reported for these source herds. in this study, individual fecal samples fr ...201627010259
drug susceptibility testing of mycobacterium avium subsp. avium isolates from naturally infected domestic pigeons to avian tuberculosis.avian tuberculosis is one of the most important infections affecting most species of birds. several mycobacterial species have been identified causing avian tuberculosis, and the organisms confirmed most frequently are mycobacterium avium and mycobacterium genavense. any species of birds can be infected with m. avium. generally, domesticated fowl or captive wild birds are affected more frequently than those living in the wild. m. avium can not only infect all species of birds, but can also infec ...201628043565
avian mycobacteriosis caused by mycobacterium avium subspecies avium in four ornamental birds and in vitro drug sensitivity testing of isolates.avian tuberculosis, one of the most important diseases affecting various species of birds, is most often caused by mycobacterium (m.) avium. this report describes cases of m. avium subsp. avium (maa) infection in a white-crested holland dwarf rooster, a male and a female golden pheasant and a male peacock. we also investigated the prevalence of mycobacteria in 60 other birds and 40 alpacas. tissue samples of necropsied birds were cultured for mycobacteria. from non-necropsied 60 other birds and ...201626904899
characterization and differentiation of mycobacterium avium subsp. paratuberculosis from other mycobacteria using matrix assisted laser desorption/ionization time-of-flight mass spectrometry.mycobacterium avium subsp. paratuberculosis (map), the causative agent of johne's disease in cattle, is responsible for significant economic losses to the us dairy industry. the pathogen has also been associated with chronic human diseases like crohn's disease, type 1 diabetes and multiple sclerosis. determining causation requires rapid characterization and source tracking the pathogen. here, we used matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry to char ...201728713782
molecular detection of mycobacterium avium avium and mycobacterium genavense in feces of free-living scarlet macaw ( ara macao) in costa rica.we conducted a study of the two main populations of free-living scarlet macaws ( ara macao) in costa rica to detect the causal agents of avian tuberculosis using noninvasive techniques. we analyzed 83 fecal samples collected between february and may 2016 from the central and southern pacific areas in the country. using pcr, we first amplified the 16s region of the rrna, common to all mycobacterium species. products from the insertion sequence is901 and from a 155-base pair (bp) dna fragment evid ...201729286261
transcriptional response of respiratory epithelium to nontuberculous mycobacteria.the incidence of pulmonary nontuberculous mycobacterial (pntm) disease is increasing, but host responses in respiratory epithelium infected with ntm are not fully understood.201728915071
non-tuberculous mycobacteria in south african wildlife: neglected pathogens and potential impediments for bovine tuberculosis diagnosis.non-tuberculous mycobacteria (ntm) are not only emerging and opportunistic pathogens of both humans and animals, but from a veterinary point of view some species induce cross-reactive immune responses that hamper the diagnosis of bovine tuberculosis (btb) in both livestock and wildlife. little information is available about ntm species circulating in wildlife species of south africa. in this study, we determined the diversity of ntm isolated from wildlife species from south africa as well as bot ...201728194371
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