| different immune response of pigs to mycobacterium avium subsp. avium and mycobacterium avium subsp. hominissuis infection. | mycobacterium avium subsp. avium (maa) and mycobacterium avium subsp. hominissuis (mah) are the most common mycobacterial species isolated from granulomatous lesions in swine in countries with controlled bovine tuberculosis. this study is focused on the immunological aspect of maa and mah infection in pigs. we detected induction of humoral and cell-mediated immunity in experimentally infected pigs. specific antibodies were analyzed in serum by elisa and the ifn-γ release assay was used for evalu ... | 2012 | 22520833 |
| mycobacterium avium subsp. avium and subsp. hominissuis give different cytokine responses after in vitro stimulation of human blood mononuclear cells. | mycobacterium avium is the principal etiologic agent of non-tuberculous lymphadenitis in children. it is also a known pathogen for birds and other animals. genetic typing of m. avium isolates has led to a proposal to expand the set of subspecies to include m. avium subsp. hominissuis. isolates associated with disease in humans belong to this subspecies. | 2012 | 22506018 |
| usability of a gamma interferon release assay in the diagnosis of naturally infected pigs with mycobacterium avium subspecies hominissuis. | in the current study, the results of an intradermal tuberculin test and a gamma interferon (ifn-γ) release assay were compared. ifn-γ release assay is based on the detection of ifn-γ production after in vitro stimulation with mycobacterium avium subsp. avium-specific antigen for the discrimination of pigs naturally infected with m. avium subsp. hominissuis. fifty-five clinically healthy pigs were used in the study. three of these were proven by culture and real-time quantitative polymerase chain ... | 2012 | 22362535 |
| effect of lipoarabinomannan from mycobacterium avium subsp avium in freund's incomplete adjuvant on the immune response of cattle. | the aim of the present study was to determine whether lipoarabinomannan (lam), in combination with freund's incomplete adjuvant (fia), was able to improve cell-mediated and antibody-mediated immune responses against ovalbumin (ova) in cattle. twenty-three calves were assigned to four treatment groups, which were subcutaneously immunized with either ova plus fia, ova plus fia and lam from mycobacterium avium subsp avium, fia plus lam, or fia alone. lymphoproliferation, ifn-γ production and cell s ... | 2012 | 22286534 |
| presence of mycobacterium avium subsp. paratuberculosis in alpacas (lama pacos) inhabiting the chilean altiplano. | mycobacterium avium subsp. paratuberculosis (map) is the etiologic agent of paratuberculosis. the organism causes disease in both domestically managed and wild ruminant species. south american camelids have a long, shared history with indigenous people in the andes. over the last few decades, increasing numbers of alpacas were exported to numerous countries outside south america. no paratuberculosis surveillance has been reported for these source herds. in this study, individual fecal samples fr ... | 2016 | 27010259 |
| molecular analysis and miru-vntr typing of mycobacterium avium subsp. avium, 'hominissuis' and silvaticum strains of veterinary origin. | besides mycobacterium avium subsp. paratuberculosis (map), m. avium subsp. avium (maa), m. avium subsp. silvaticum (mas), and 'm. avium subsp. hominissuis' (mah) are equally important members of m. avium complex, with worldwide distribution and zoonotic potential. genotypic discrimination is a prerequisite to epidemiological studies which can facilitate disease prevention through revealing infection sources and transmission routes. the primary aim of this study was to identify the genetic divers ... | 2016 | 26964909 |
| mycobacterial infections in rabbits: from the wild to the laboratory. | tuberculous mycobacterial diseases such as leprosy and tuberculosis are ancient diseases that currently continue threatening human health in some countries. non-tuberculous mycobacterial (ntm) infections cause a series of well-defined pathological entities, as well as some opportunistic diseases that have also increased worldwide, being more common among immunocompromised patients but rising also in immunocompetent individuals. reports on natural infections by mycobacteria in rabbits are scarce ... | 2016 | 26799551 |
| detection of mycobacterium avium subspecies in the gut associated lymphoid tissue of slaughtered rabbits. | rabbits are susceptible to infection by different species of the genus mycobacterium. particularly, development of specific lesions and isolation of mycobacterium avium subsp. avium and mycobacterium avium subsp. paratuberculosis, both subspecies of the m. avium complex, has been reported in wildlife conditions. although, rabbit meat production worldwide is 200 million tons per year, microbiological data on this source of meat is lacking and more specifically reports of mycobacterial presence in ... | 2015 | 26063469 |
| mycobacterium avium subsp. avium infection in four veal calves: differentiation from intestinal tuberculosis. | mycobacterium avium subsp. avium (maa) is an intracellular pathogen belonging to the mycobacterium avium-intracellulare complex (mac). reservoirs of mac are the natural environment, wildlife and domestic animals. in adult bovine, mac infections are typically caused by mycobacterium avium subsp. paratuberculosis (map). maa infections in bovine are rarely reported but may cause clinical disease and pathological lesions similar to those observed in paratuberculosis or those induced by members of th ... | 2014 | 24689051 |
| analysis of sediments and plants from the system of five fishponds in the czech republic using culture and pcr methods. | nontuberculous mycobacteria (ntm) are ubiquitous organisms that have been isolated from a variety of environmental sources. several ntm species are responsible for diseases in humans and/or animals known as mycobacterioses. the aim of this study was to determine the levels of ntm in the sediments and plants of five fish ponds in the czech republic using culture and quantitative real time pcr (qpcr). additionally, we investigated if there was any link between environmental samples from the fish p ... | 2014 | 24342091 |
| mannosylated lipoarabinomannans from mycobacterium avium subsp. paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of mycobacterium avium subsp. avium organisms. | analysis of the mechanisms through which pathogenic mycobacteria interfere with macrophage activation and phagosome maturation have shown that engagement of specific membrane receptors with bacterial ligands is the initiating event. mannosylated lipoarabinomannan (man-lam) has been identified as one of the ligands that modulates macrophage function. we evaluated the effects of man-lam derived from mycobacterium avium subsp. paratuberculosis (map) on bovine macrophages. man-lam induced a rapid an ... | 2013 | 24098744 |
| novel feature of mycobacterium avium subsp. paratuberculosis, highlighted by characterization of the heparin-binding hemagglutinin adhesin. | mycobacterium avium subsp. paratuberculosis comprises two genotypically defined groups, known as the cattle (c) and sheep (s) groups. recent studies have reported phenotypic differences between m. avium subsp. paratuberculosis groups c and s, including growth rates, infectivity for macrophages, and iron metabolism. in this study, we investigated the genotypes and biological properties of the virulence factor heparin-binding hemagglutinin adhesin (hbha) for both groups. in mycobacterium tuberculo ... | 2013 | 23974028 |
| [production and evaluation of a purified protein derivative from an argentine strain of mycobacterium avium subsp. paratuberculosis]. | purified protein derivatives (ppds) are non-defined antigens prepared from mycobacteria cultures. they are usually employed to evaluate the specific cellular immune response both in animals and humans. bovine and avian ppds are usually employed as antigens in mycobacterial infections such as tuberculosis and paratuberculosis. nevertheless, ppd from mycobacterium avium subsp. paratuberculosis, (ppdj) is neither commonly used nor frequently available. however, ppd from mycobacterium avium subsp. a ... | 2015 | 23102462 |
| production and proteomic characterisation of purified protein derivative from mycobacterium avium subsp. paratuberculosis. | effective diagnosis of johne's disease (jd), particularly at the stage of early subclinical infection, remains one of the greatest challenges for the control of jd worldwide. the ifn-γ test of cell mediated immunity is currently one of the most suitable diagnostics for subclinical infections, however a major limitation of this test is the lack of a standardised purified protein derivative (ppd) antigen (also referred to as johnin ppd or ppdj). while attempting to replace ppdj with more specific ... | 2012 | 22443541 |
| characterization and differentiation of mycobacterium avium subsp. paratuberculosis from other mycobacteria using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. | mycobacterium avium subsp. paratuberculosis (map), the causative agent of johne's disease in cattle, is responsible for significant economic losses to the us dairy industry. the pathogen has also been associated with chronic human diseases like crohn's disease, type 1 diabetes and multiple sclerosis. determining causation requires rapid characterization and source tracking the pathogen. here, we used matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry to char ... | 2017 | 28713782 |
| molecular detection of mycobacterium avium avium and mycobacterium genavense in feces of free-living scarlet macaw ( ara macao) in costa rica. | we conducted a study of the two main populations of free-living scarlet macaws ( ara macao) in costa rica to detect the causal agents of avian tuberculosis using noninvasive techniques. we analyzed 83 fecal samples collected between february and may 2016 from the central and southern pacific areas in the country. using pcr, we first amplified the 16s region of the rrna, common to all mycobacterium species. products from the insertion sequence is901 and from a 155-base pair (bp) dna fragment evid ... | 2017 | 29286261 |
| transcriptional response of respiratory epithelium to nontuberculous mycobacteria. | the incidence of pulmonary nontuberculous mycobacterial (pntm) disease is increasing, but host responses in respiratory epithelium infected with ntm are not fully understood. | 2017 | 28915071 |